CN112674901A - Tool and method for batch radioactive skin injury experiment of animals - Google Patents
Tool and method for batch radioactive skin injury experiment of animals Download PDFInfo
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- CN112674901A CN112674901A CN202011503540.3A CN202011503540A CN112674901A CN 112674901 A CN112674901 A CN 112674901A CN 202011503540 A CN202011503540 A CN 202011503540A CN 112674901 A CN112674901 A CN 112674901A
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Abstract
The invention relates to a tool and a method for batch radioactive skin injury experiments of animals. The instrument can once carry out the construction of many rats skin injury models, through the aluminium shielding apron, can effectively reduce the influence of the bremsstrahlung radiation that produces to the rat among the electron beam irradiation process, through setting up of irradiation hole size on the apron, can construct the radioactive skin injury rat model of self-healing.
Description
Technical Field
The invention relates to the field of basic research and application of radioactive skin injury treatment, in particular to a tool and a method for batch animal radioactive skin injury experiments.
Background
With the widespread application of nuclear technology in various social fields, radioactive skin injuries are increased correspondingly and become a common clinical disease. The radioactive skin injury is mainly the injury of epidermis and dermis, the basic lesion is the damage of epithelial layer cells and the injury of vascular endothelial cells, the wound healing of the damage is delayed, and the damage is a classic wound model which is difficult to heal.
At present, although the radioactive skin injury treatment means are various, the effect after healing is not ideal. Many drugs can protect the wound surface to different degrees, prevent infection and promote cell proliferation. But the problems of thin, dry, severe pruritus and easy repeated ulceration of the skin at the later stage of long-term healing are not fundamentally solved. At present, scientific research and clinical trials are actively carried out aiming at the difficulty of repeated ulceration of the wound surface of radioactive skin injury in clinical and related scientific research. In the research process, the preparation of the radioactive skin injury model is the key. The radioactive skin injury model is only limited to skin injury, other tissues and internal organs of animals cannot be injured, and meanwhile, the constructed injury model can realize self-healing. At present, a tool structure specially aiming at the animal radioactive skin injury experiment does not exist, so that a tool for the animal batch radioactive skin injury experiment is in urgent need to appear.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a tool and a method for batch radioactive skin injury experiments of animals.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the utility model provides a instrument that is used for animal batch radioactivity skin damage experiment, is shining the apron at cage utensil frame top including shining cage utensil frame and closing cap, shine cage utensil frame and include bottom plate and curb plate, shine the open-top of cage utensil frame, shine and be equipped with horizontal baffle and longitudinal baffle in the cage utensil frame, horizontal baffle and longitudinal baffle will shine the inner chamber of cage utensil frame and separate and place the chamber for a plurality of animals, be equipped with a plurality of irradiation holes of placing the chamber and corresponding with the animal on the apron.
Furthermore, a plurality of air holes communicated with the animal placing cavity are formed in a side plate or a bottom plate of the irradiation cage frame.
Furthermore, the number of the animal placing cavities is eight, the irradiation holes correspond to the animal placing cavities one to one, and the irradiation holes are located at one end of the top of the animal placing cavities.
Further, the cover plate is made of an aluminum plate.
Furthermore, the irradiation cage frame is made of stainless steel.
A method for batch radioactive skin injury testing of animals, comprising the steps of:
s1, selecting 8 male inbred line clean-grade SD rats with the age of 5-6 weeks, and weighing, marking and numbering the rats one day before irradiation after 7-day quarantine;
s2, before the experiment, 2.5 percent sodium pentobarbital is adopted for the rats to be anesthetized by intraperitoneal injection (0.2 ml of 2.5 percent sodium pentobarbital is injected per 100 g);
s3, after the anesthesia effect is obvious, shaving the skin on the back of the rat hip, and putting the rat into an animal placing cavity of the irradiation cage frame to enable the skin on the back of the rat hip to be positioned at the irradiation hole on the cover plate;
s4, covering a cover plate, placing the whole irradiation cage frame on a medical accelerator treatment bed, ensuring that an irradiation hole area is positioned in an accelerator electron beam irradiation area, and irradiating 45Gy at one time by using a 4MeV electron beam generated by an accelerator;
s5, after irradiation, taking out the rat, placing the rat in a rearing cage, and allowing the rat to naturally revive.
The invention has the beneficial effects that: this instrument is fit for fixing big experimental animals in batches, and the experiment is shone to the local skin of animal to the 4MeV electron line that the hole of shining that sets up on the apron produced the convenient accelerator, provides the experimental data support to radioactive skin damage treatment, has established radioactive skin damage model, simple structure, and is practical. In addition, the tool can be used for constructing a plurality of rat skin injury models at one time, the influence of bremsstrahlung generated in the electron beam irradiation process on rats can be effectively reduced through the aluminum shielding cover plate, and the self-healing radioactive skin injury rat model can be constructed through the arrangement of the sizes of the irradiation holes in the cover plate.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of an irradiation cage frame according to the present invention.
Detailed Description
As shown in fig. 1 and fig. 2, a tool for batch radioactive skin injury experiments of animals comprises an irradiation cage frame 1 and a cover plate 2 which is covered on the top of the irradiation cage frame 1, wherein the irradiation cage frame 1 comprises a bottom plate 11 and a side plate 12, the top of the irradiation cage frame 1 is open, a transverse partition plate 13 and a longitudinal partition plate 14 are arranged in the irradiation cage frame 1, the transverse partition plate 13 and the longitudinal partition plate 14 divide an inner cavity of the irradiation cage frame 1 into a plurality of animal placing cavities 15, and a plurality of irradiation holes 21 corresponding to the animal placing cavities 15 are arranged on the cover plate 2.
Furthermore, a plurality of air holes 16 communicated with the animal placing cavity 15 are formed in the side plate 12 or the bottom plate 11 of the irradiation cage frame, so that the normal breathing of the animals in the animal placing cavity 15 is ensured. The number of the animal placing cavities 15 is eight, the irradiation holes 21 correspond to the animal placing cavities 15 one by one, and the irradiation holes 21 are located at one end of the top of the animal placing cavities 15.
In this embodiment, the parameters of the tool are as follows:
1. the irradiation cage frame 1 is constructed from stainless steel and the cover plate 2 is made from 1.5cm thick aluminium plate.
2. The irradiation cage has an overall size of 50cm × 25cm × 10cm (length × width × height), and two cover plates 2 each having a size of 25cm × 25cm × 1.5cm (length × width × thickness).
3. 4 irradiation holes 21 (with the diameter of 3.2cm) are reserved on each cover plate 2, the two cover plates have 8 irradiation holes 21 in total, and the irradiation holes 21 are positioned in an area within the range of 20cm multiplied by 20cm (considering the size of the irradiation field of the accelerator).
4. The cage has the characteristics that: 8 rats can be irradiated at a time; the aluminum cover plate shields the unirradiated area, so that the damage of bremsstrahlung can be effectively reduced; the rat irradiation field is circular with the diameter of 3.2cm, and the wound surface can be self-healed after irradiation.
Further, the method for the animal batch radioactive skin injury experiment comprises the following steps:
s1, selecting 8 male inbred line clean-grade SD rats with the age of 5-6 weeks, and weighing, marking and numbering the rats one day before irradiation after 7-day quarantine;
s2, before the experiment, 2.5 percent sodium pentobarbital is adopted for the rats to be anesthetized by intraperitoneal injection (0.2 ml of 2.5 percent sodium pentobarbital is injected per 100 g);
s3, after the anesthesia effect is achieved, shaving the skin of the back of the hip of the rat, and putting the rat into the animal placing cavity 15 of the irradiation cage frame to enable the skin of the back of the hip of the rat to be located at the irradiation hole 21 on the cover plate 2;
s4, covering the cover plate 2, placing the whole irradiation cage frame on a medical accelerator treatment bed, ensuring that the irradiation hole area is positioned in an accelerator electron beam irradiation area, and irradiating 45Gy at one time by using a 4MeV electron beam generated by an accelerator;
s5, after irradiation, taking out the rat, placing the rat in a rearing cage, and allowing the rat to naturally revive.
This instrument is fit for fixing big experimental animals in batches, and the experiment is shone to the local skin of animal to the 4MeV electron line that the hole of shining that sets up on the apron produced the convenient accelerator, provides the experimental data support to radioactive skin damage treatment, has established radioactive skin damage model, simple structure, and is practical. In addition, the tool can be used for constructing a plurality of rat skin injury models at one time, the influence of bremsstrahlung generated in the electron beam irradiation process on rats can be effectively reduced through the aluminum shielding cover plate, and the self-healing radioactive skin injury rat model can be constructed through the arrangement of the sizes of the irradiation holes in the cover plate.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. The utility model provides a instrument that is used for animal batch radioactivity skin damage experiment, is shining the apron at cage utensil frame top including shining cage utensil frame and closing cap, its characterized in that, it includes bottom plate and curb plate to shine the cage utensil frame, shines the open-top of cage utensil frame, shines and is equipped with horizontal partition and longitudinal baffle in the cage utensil frame, and horizontal partition and longitudinal baffle will shine the inner chamber partition of cage utensil frame and place the chamber for a plurality of animals, be equipped with a plurality of irradiation holes of placing the chamber with the animal on the apron and correspond.
2. The tool for batch radioactive skin injury experiments on animals according to claim 1, wherein a plurality of air holes communicated with the animal placing cavity are formed on a side plate or a bottom plate of the irradiation cage frame.
3. The tool for batch radioactive skin injury test of animals according to claim 1 or 2, wherein the number of the animal placing cavities is eight, the irradiation holes correspond to the animal placing cavities one by one, and the irradiation holes are located at one end of the top of the animal placing cavities.
4. The tool for batch animal radioactive skin injury experiments according to claim 3, wherein the cover plate is made of aluminum plate.
5. The tool for batch radioactive skin injury experiments on animals according to claim 3, wherein the frame of the irradiation cage is made of stainless steel.
6. A method for batch radioactive skin injury testing of animals, comprising the steps of:
s1, selecting 8 male inbred line clean-grade SD rats with the age of 5-6 weeks, and weighing, marking and numbering the rats one day before irradiation after 7-day quarantine;
s2, before the experiment, 2.5 percent sodium pentobarbital is adopted for the rat to be anesthetized by intraperitoneal injection;
s3, after the anesthesia effect is obvious, shaving the skin on the back of the rat hip, and putting the rat into an animal placing cavity of the irradiation cage frame to enable the skin on the back of the rat hip to be positioned at the irradiation hole on the cover plate;
s4, covering a cover plate, placing the whole irradiation cage frame on a medical accelerator treatment bed, ensuring that an irradiation hole area is positioned in an accelerator electron beam irradiation area, and irradiating 45Gy at one time by using a 4MeV electron beam generated by an accelerator;
s5, after irradiation, taking out the rat, placing the rat in a rearing cage, and allowing the rat to naturally revive.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011503540.3A CN112674901A (en) | 2020-12-18 | 2020-12-18 | Tool and method for batch radioactive skin injury experiment of animals |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011503540.3A CN112674901A (en) | 2020-12-18 | 2020-12-18 | Tool and method for batch radioactive skin injury experiment of animals |
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| CN112674901A true CN112674901A (en) | 2021-04-20 |
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| CN202011503540.3A Pending CN112674901A (en) | 2020-12-18 | 2020-12-18 | Tool and method for batch radioactive skin injury experiment of animals |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0531131A (en) * | 1991-07-30 | 1993-02-09 | Hitachi Medical Corp | Housing cage for experimental small animal |
| CN202892670U (en) * | 2012-10-22 | 2013-04-24 | 上海交通大学医学院附属第九人民医院 | Device of constructing rat lower limb osteoradionecrosis model |
| JP2013118982A (en) * | 2011-12-08 | 2013-06-17 | Natl Inst Of Radiological Sciences | Isolation container, connection device for carrying-in/out, management system for animal isolation, and method for irradiating radiation to isolation-reared animal |
| CN203341845U (en) * | 2013-07-02 | 2013-12-18 | 王琛 | Small animal sobering and fixing device used for experiment |
| CN209203604U (en) * | 2018-04-25 | 2019-08-06 | 中国人民解放军第四军医大学 | Fixation cage tool for batch whole body irradiation mouse |
-
2020
- 2020-12-18 CN CN202011503540.3A patent/CN112674901A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0531131A (en) * | 1991-07-30 | 1993-02-09 | Hitachi Medical Corp | Housing cage for experimental small animal |
| JP2013118982A (en) * | 2011-12-08 | 2013-06-17 | Natl Inst Of Radiological Sciences | Isolation container, connection device for carrying-in/out, management system for animal isolation, and method for irradiating radiation to isolation-reared animal |
| CN202892670U (en) * | 2012-10-22 | 2013-04-24 | 上海交通大学医学院附属第九人民医院 | Device of constructing rat lower limb osteoradionecrosis model |
| CN203341845U (en) * | 2013-07-02 | 2013-12-18 | 王琛 | Small animal sobering and fixing device used for experiment |
| CN209203604U (en) * | 2018-04-25 | 2019-08-06 | 中国人民解放军第四军医大学 | Fixation cage tool for batch whole body irradiation mouse |
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