CN112647317A - 一种提高羊毛纤维强力的生物酶促染色方法 - Google Patents

一种提高羊毛纤维强力的生物酶促染色方法 Download PDF

Info

Publication number
CN112647317A
CN112647317A CN202011510926.7A CN202011510926A CN112647317A CN 112647317 A CN112647317 A CN 112647317A CN 202011510926 A CN202011510926 A CN 202011510926A CN 112647317 A CN112647317 A CN 112647317A
Authority
CN
China
Prior art keywords
wool
dyeing
wool fibers
yarn
laccase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202011510926.7A
Other languages
English (en)
Other versions
CN112647317B (zh
Inventor
苏静
王鸿博
李洁
李宇
李楠楠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangnan University
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN202011510926.7A priority Critical patent/CN112647317B/zh
Publication of CN112647317A publication Critical patent/CN112647317A/zh
Application granted granted Critical
Publication of CN112647317B publication Critical patent/CN112647317B/zh
Priority to PCT/CN2022/073958 priority patent/WO2022127940A1/zh
Priority to US17/873,463 priority patent/US20220356643A1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P3/00—Special processes of dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form, classified according to the material treated
    • D06P3/02—Material containing basic nitrogen
    • D06P3/04—Material containing basic nitrogen containing amide groups
    • D06P3/14—Wool
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P3/00—Special processes of dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form, classified according to the material treated
    • D06P3/02—Material containing basic nitrogen
    • D06P3/04—Material containing basic nitrogen containing amide groups
    • D06P3/14—Wool
    • D06P3/148—Wool using reactive dyes
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
    • D06M11/73—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof
    • D06M11/76—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising with carbon or compounds thereof with carbon oxides or carbonates
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M10/00—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements
    • D06M10/02—Physical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. ultrasonic, corona discharge, irradiation, electric currents, or magnetic fields; Physical treatment combined with treatment with chemical compounds or elements ultrasonic or sonic; Corona discharge
    • D06M10/025—Corona discharge or low temperature plasma
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
    • D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
    • D06M13/152—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen having a hydroxy group bound to a carbon atom of a six-membered aromatic ring
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
    • D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
    • D06M13/224—Esters of carboxylic acids; Esters of carbonic acid
    • D06M13/238—Tannins, e.g. gallotannic acids
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/0004—General aspects of dyeing
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/34—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using natural dyestuffs
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/38—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using reactive dyes
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/44—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/44—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders
    • D06P1/64—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders using compositions containing low-molecular-weight organic compounds without sulfate or sulfonate groups
    • D06P1/651—Compounds without nitrogen
    • D06P1/65106—Oxygen-containing compounds
    • D06P1/65112—Compounds containing aldehyde or ketone groups
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/44—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders
    • D06P1/64—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders using compositions containing low-molecular-weight organic compounds without sulfate or sulfonate groups
    • D06P1/651—Compounds without nitrogen
    • D06P1/65106—Oxygen-containing compounds
    • D06P1/65118—Compounds containing hydroxyl groups
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/44—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using insoluble pigments or auxiliary substances, e.g. binders
    • D06P1/673—Inorganic compounds
    • D06P1/67333—Salts or hydroxides
    • D06P1/6735—Salts or hydroxides of alkaline or alkaline-earth metals with anions different from those provided for in D06P1/67341
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P3/00—Special processes of dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form, classified according to the material treated
    • D06P3/02—Material containing basic nitrogen
    • D06P3/04—Material containing basic nitrogen containing amide groups
    • D06P3/14—Wool
    • D06P3/16—Wool using acid dyes
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
    • D06P5/20—Physical treatments affecting dyeing, e.g. ultrasonic or electric
    • D06P5/2016—Application of electric energy
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P5/00—Other features in dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form
    • D06P5/22—Effecting variation of dye affinity on textile material by chemical means that react with the fibre

Landscapes

  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plasma & Fusion (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Coloring (AREA)

Abstract

本发明公开了一种提高羊毛纤维强力的生物酶促染色方法，属于纺织材料改性技术领域。本发明利用蛋白质纤维大分子中含有大量的羟基、氨基和羧基等活性基团，易与酶催化酚类化合物形成的聚酚类色素反应形成共价结合的特点，实现羊毛纤维低温上染的同时提升纤维强力。本发明操作条件温和，易控制，在日益强调环保的今天，利用生物酶对羊毛纤维染色安全、环保、高效、有着长远的发展前景。

Description

一种提高羊毛纤维强力的生物酶促染色方法
技术领域
本发明涉及一种提高羊毛纤维强力的生物酶促染色方法，属于纺织材料改性技术。
背景技术
蛋白质纤维如羊毛纤维具有优良的吸湿性、保暖性，穿着柔软，在纺织行业应用广泛。在加工过程中，如洗毛、纺纱、织造、染色等，纤维会受到损伤，机械性能变差，值得注意的是，因羊毛表面鳞片层具有疏水性，常使用高温工艺进行染色，造成强力损伤,严重影响纤维的后续加工和织物的服用性能，且能耗大，对环境不利。因此寻求一种温和有效的染色方法预防和修复羊毛纤维损伤具有重要意义。
现有技术中，对羊毛纤维强力损伤修复的报道较少，有报道使用化学交联剂，如乙二醛、戊二醛、二辛酯和碳二亚胺等，这些交联剂便宜，且容易提高纤维的拉伸性能，但对环境、对使用者的健康构成极大的威胁。目前，针对转谷氨酰胺酶(TG酶)修复羊毛强力损伤的研究较广泛，TG酶具有吸收伯胺和将肽链(含谷氨酰胺或赖氨酸残基)嫁接到蛋白质中的能力，可修复羊毛的化学损伤、生物损伤等多重损伤，但用途单一。
蛋白质纤维染色主要以酸性染料与活性染料为主，通过与纤维发生物理或化学结合上染。但随着世界能源枯竭及环境问题的日益恶化，合成染料和印染工业的发展面临着环境污染、高人工成本等众多问题，寻找新型生态友好型染料成为新的发展方向。
发明内容
解决的技术问题：
本发明的生物酶促染色及提高羊毛纤维强力的方法，首先用物理或化学方法对羊毛纱线进行预处理，然后使经预处理的羊毛纱线在漆酶-酚类化合物的反应体系中进行染色，蛋白质纤维大分子中含有大量的羟基、氨基和羧基等活性基团，易与有色酶促酚类聚合产物反应形成共价结合，从而实现对羊毛纤维低温染色并赋予纤维良好的染色性能与力学性能，反应条件温和，易控制，绿色环保，有广阔的发展前景。
本发明使用天然的动植物染料代替化学染料。漆酶是一种含铜的氧化还原酶，存在于许多植物、真菌和微生物中。在纺织加工中，漆酶被用于提高漂白过程中织物的白度、有色废水的脱色以及纤维的精练、羊毛防毡缩等，但未有用于改善增强羊毛纤维强力的报道，本发明利用漆酶可催化底物反应生成有色聚合产物，与蛋白质纤维大分子中大量的羟基、氨基和羧基等活性基团反应形成共价结合，在完成原位上染的同时提升羊毛纤维强力。
技术方案：
本发明的第一个目的是提供一种生物酶促提高羊毛纤维强力并染色的方法，所述生物酶促染色及提高羊毛纤维强力的方法是：将漆酶-酚类化合物加入到缓冲溶液中，羊毛纤维在含有漆酶-酚类化合物的反应体系中进行染色。
在本发明的一种实施方式中，所述酚类化合物为邻苯二酚、对苯二酚、没食子酸、香草醛、愈创木酚等。
在本发明的一种实施方式中，酶催化酚类化合物与羊毛反应前，羊毛纤维需经过预处理：所述预处理包括化学法、酶法或物理法。
在本发明的一种实施方式中，所述预处理可采用碳酸钠进行预处理，所述处理工艺处方及条件：称取羊毛纤维和碳酸钠，将碳酸钠溶解在去离子水中配制成0.5-1g/L的碳酸钠溶液，浴比为1：20～1：50。将羊毛纤维浸泡在30-80℃的碳酸钠溶液中处理15-30min，然后用40℃的无水乙醇清洗5-15min,去离子水漂洗多次，将洗净的羊毛纤维在40-60℃下烘干。
在本发明的一种实施方式中，所述预处理可采用低温等离子体进行预处理，所述处理工艺处方及条件：将羊毛纤维置于低温等离子体处理机内架子上，开启真空泵，通入一路氧气，点击自动模式对羊毛纤维进行低温等离子体处理，处理功率100-150W，处理时间5-10min。
在本发明的一种实施方式中，所述生物酶促染色及提高羊毛纤维强力的方法为：将生物催化剂漆酶、酚类化合物依次加入到缓冲溶液中，配制成pH为4.0-6.0的反应体系，将经过预处理的羊毛纤维加入到反应体系中，于30-80℃下反应2-10小时。
在本发明的一种实施方式中，所述缓冲溶液为乙酸-乙酸钠缓冲液。
在本发明的一种实施方式中，所述乙酸-乙酸钠缓冲溶液浓度为0.1-0.3mol/L。
在本发明的一种实施方式中，所述生物酶促染色及提高羊毛纤维强力的工艺处方及条件：配制pH为4.0-6.0的乙酸-乙酸钠缓冲溶液，向缓冲溶液中依次加入催化剂漆酶与底物，使漆酶浓度为25～125U/mL，酚类化合物浓度为0.04-0.20mol/L，将经过预处理的羊毛纤维加入到反应体系中，浴比为1：20-1：50，升温至30-80℃保温反应2～10小时。
在本发明的一种实施方式中，所述漆酶的用量为(25～125U)U/(0.04-0.20)mmol酚类化合物。
本发明的第二个目的是提供一种采用上述方法制备得到的蛋白质纤维。
在本发明的一种实施方式中，所述蛋白质纤维为羊毛。
本发明的第三个目的是提供一种含有上述羊毛的纱、线和织物。
有益效果：
本发明利用生物催化剂--漆酶催化氧化酚类化合物生成有色聚合产物，蛋白质纤维大分子中含有大量的羟基、氨基和羧基等活性基团，易与酶促酚类有色聚合产物反应形成共价结合，从而实现羊毛纤维染色，并赋予羊毛良好的染色性能，染色后的羊毛强力显著提高，耐碱性能改善，且具有抗氧化性能。该反应条件温和，易控制，对环境友好，有良好的发展前景。
附图说明
为了更清楚地说明本发明具体实施方式或现有技术中的技术方案，下面将对具体的实施方式或现有技术描述中所使用的附图做简单地介绍，显而易见地，下面描述中的附图是本发明的一些实施方式，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他附图。
图1为未改性羊毛纱线和改性羊毛纱线的SEM图，标尺均为10μm,其中，a为空白对照组，b为实施例1,c为实施例2。
图2为未改性羊毛纱线和改性羊毛纱线横截面(放大1000倍)图，其中，a为空白对照组，b为实施例1,c为实施例2。
图3为未改性羊毛纱线和改性羊毛纱线染色图，其中，a为空白对照组，b为实施例1,c为实施例2。
图4为生物酶促染色过程示意图。
具体实施方式
以下对本发明的优选实施例进行说明，应当理解实施例是为了更好地解释本发明，不用于限制本发明。
断裂强力测试：在拉伸试验机上进行，选择加持长度180mm，拉伸拉伸速度为100mm/min,，测试多次，取平均值。碱溶性测定：用碱处理后纱线的重量和溶解度公式计算纱线的溶解度，测试纱线抗碱损伤性能。抗氧化性测定：采用ABTS法测定纱线的抗氧化性能。羊毛纱线的K/S值用测色仪测试。
实施例1
(1)预处理：
所述处理工艺处方及条件：称取1g羊毛纱线和碳酸钠，将碳酸钠溶解在去离子水中配制成1g/L的碳酸钠溶液，浴比为1：30。将羊毛纤维浸泡在40℃的碳酸钠溶液中处理15min，然后用40℃的无水乙醇清洗10min,去离子水漂洗，将洗净的羊毛纤维在40℃下烘干。
(2)酶催化酚类化合物与羊毛反应：
配制溶液：配制0.2mol/L的乙酸-乙酸钠缓冲液，调节pH至5.0，依次加入0.04mol/L邻苯二酚和75U/mL漆酶，浴比1：30，置于反应器中震荡均匀，将经预处理过的羊毛纱线放入配制好的缓冲液中，于40℃下恒温振荡反应5h。
(3)后处理：
将步骤(3)得到的羊毛纱线取出后，在-50℃条件下冷冻12h灭酶，用去离子水清洗，自然晾干。
实施例2
(1)预处理：
所述处理工艺处方及条件：将1g羊毛纱线置于低温等离子体处理机内，开启真空泵，通入一路氧气，点击自动模式，对蛋白质纤维进行低温等离子体处理，处理功率100W，处理时间5min。
(2)酶催化酚类化合物与羊毛反应：
配制溶液：配制0.2mol/L的乙酸-乙酸钠缓冲液，调节pH至5.5，依次加入0.04mol/L邻苯二酚和75U/mL漆酶，浴比1：50，置于反应器中震荡均匀，将经预处理过的羊毛纱线放入配制好的缓冲液中，于40℃下恒温振荡反应5h。
(3)后处理：
将步骤(3)得到的羊毛纱线取出后，在-40℃条件下冷冻12h灭酶，然后用去离子水清洗，自然晾干。
对实施例1-2所得的羊毛的性能进行检测，结果见表1和表2。
表1实施例1-2所得的羊毛纤维的性能检测结果
Figure BDA0002846377280000041
表2实施例1-2中不同处理方法所得的羊毛纤维的染色参数
Figure BDA0002846377280000042
Figure BDA0002846377280000051
根据表中数据可见,经不同预处理的羊毛纱线进行酶促聚合染色都可得到较好的染色深度，且羊毛纱线强力均得到不同程度的提高，碱溶性降低，抗氧化性提高。较为明显的是，经等离子体预处理后的酶法染色羊毛纱线强力提升了26％，较化学预处理的酶促聚合染色羊毛纱线力学性能好。
实施例3探究漆酶用量对羊毛纤维染色的影响
参照实施例1，将步骤(2)中的漆酶的用量分别替换为25U/mL、50U/mL、100U/mL、125U/mL，其他条件不变：
(1)预处理：
所述处理工艺处方及条件：称取1g羊毛纱线和碳酸钠，将碳酸钠溶解在去离子水中配制成1g/L的碳酸钠溶液，浴比为1：30。将羊毛纤维浸泡在40℃的碳酸钠溶液中处理15min，然后用40℃的无水乙醇清洗10min,去离子水漂洗，将洗净的羊毛纤维在40℃下烘干。
(2)酶催化酚类化合物与羊毛反应：
配制溶液：配制0.2mol/L的乙酸-乙酸钠缓冲液，调节pH至5.0，依次加入0.04mol/L邻苯二酚和25U/mL、50U/mL、75U/mL、100U/mL、125U/mL漆酶，浴比1：30，置于反应器中震荡均匀，将经预处理过的羊毛纱线放入配制好的缓冲液中，于40℃下恒温振荡反应5h。
(3)后处理：
将步骤(3)得到的羊毛纱线取出后，在-50℃条件下冷冻12h灭酶，用去离子水清洗，自然晾干。
所得的羊毛的性能进行检测，结果见表3、4。
表3不同酶浓度所得染色羊毛纤维的结果
Figure BDA0002846377280000052
Figure BDA0002846377280000061
表4不同酶浓度所得的羊毛纤维的染色参数
Figure BDA0002846377280000062
根据表中数据可见，当酶浓度较低时，纱线即可得到较高的染色深度和断裂强度，L*值逐渐降低(明度降低)，a*、b*均为正值，纱线呈现棕褐色。随着酶浓度增加，纱线碱溶性减少，抗氧化性虽略有增加，但效果也不佳。当酶浓度超过75U/mL，断裂强力和染色深度均趋于稳定。漆酶浓度太高时(150U/mL)，染色深度稍有变深、但强力改善效果下降，且浪费试剂，不经济。
实施例4探究邻苯二酚浓度对羊毛纤维染色的影响
参照实施例1，将步骤(2)中的酚类底物的反应浓度分别替换为0.08mol/L、0.12mol/L、0.16mol/L、0.20mol/L，其他条件不变：
(1)预处理：
所述处理工艺处方及条件：称取1g羊毛纱线和碳酸钠，将碳酸钠溶解在去离子水中配制成1g/L的碳酸钠溶液，浴比为1：30。将羊毛纤维浸泡在40℃的碳酸钠溶液中处理15min，然后用40℃的无水乙醇清洗10min,去离子水漂洗，将洗净的羊毛纤维在40℃下烘干。
(2)酶催化酚类化合物与羊毛反应：
配制溶液：配制0.2mol/L的乙酸-乙酸钠缓冲液，调节pH至5.0，依次加入0.08mol/L、0.12mol/L、0.16mol/L、0.20mol/L邻苯二酚和75U/mL漆酶，浴比1：30，置于反应器中震荡均匀，将经预处理过的羊毛纱线放入配制好的缓冲液中，于40℃下恒温振荡反应5h。
(3)后处理：
将步骤(3)得到的羊毛纱线取出后，在-50℃条件下冷冻12h灭酶，用去离子水清洗，自然晾干。
所得的羊毛的性能进行检测，结果见表5、6。
表5不同邻苯二酚浓度所得染色羊毛纤维的结果
Figure BDA0002846377280000071
表6不同邻苯二酚浓度所得的羊毛纤维的染色参数
Figure BDA0002846377280000072
根据表中数据可见，在漆酶的催化作用下，随着邻苯二酚单体浓度的增加，羊毛纱线的染色深度逐渐增加，纱线呈现棕褐色，且耐碱性与抗氧化性改善。但随着酚用量的增加，纱线的断裂强力先增加后减小，断裂伸长率随着酚用量增加而减小。当底物浓度低(比如0.02mol/L)时，染色及强力没有显著改善效果；底物浓度过高(比如0.4mol/L)时，染色深度变大，但染色不匀，纱线强力下降、断裂伸长下降，手感***弹性变差。
实施例5探究反应温度对羊毛纤维染色的影响
参照实施例1，将步骤(2)中的反应温度分别替换为30℃、50℃、60℃、70℃、80℃，其他条件不变：
(1)预处理：
所述处理工艺处方及条件：称取1g羊毛纱线和碳酸钠，将碳酸钠溶解在去离子水中配制成1g/L的碳酸钠溶液，浴比为1：30。将羊毛纤维浸泡在40℃的碳酸钠溶液中处理15min，然后用40℃的无水乙醇清洗10min，去离子水漂洗，将洗净的羊毛纤维在40℃下烘干。
(2)酶催化酚类化合物与羊毛反应：
配制溶液：配制0.2mol/L的乙酸-乙酸钠缓冲液，调节pH至5.0，依次加入0.04mol/L邻苯二酚和75U/mL漆酶，浴比1：30，置于反应器中震荡均匀，将经预处理过的羊毛纱线放入配制好的缓冲液中，于不同温度下恒温振荡反应5h。
(3)后处理：
将步骤(3)得到的羊毛纱线取出后，在-50℃条件下冷冻12h灭酶，用去离子水清洗，自然晾干。
所得的羊毛的性能进行检测，结果见表7、8。
表7不同温度所得染色羊毛纤维的结果
Figure BDA0002846377280000081
表8不同温度所得的羊毛纤维的染色参数
Figure BDA0002846377280000082
根据表中数据可见，当温度低于50℃时，染色纱线染色深度和断裂强度随着温度的升高而增加，温度高于50℃后，羊毛纱线的断裂强度、染色深度随温度升高缓慢降低。
虽然本发明已以较佳实例公开如上，但其并非用以限定本发明，任何熟悉此技术的人，在不脱离本发明的精神和范围内，都可做各种的改动与修饰，因此本发明的保护范围应该以权力书所界定的为准。

Claims (10)

1.一种生物酶促提高羊毛纤维强力的方法，其特征在于，所述方法是将漆酶、酚类化合物加入到缓冲溶液中，形成混合体系；然后将羊毛纤维放置在所得混合体系中，30-80℃下保温进行反应。
2.根据权利要求1所述的方法，其特征在于，所述酚类化合物为邻苯二酚、对苯二酚、没食子酸、香草醛、愈创木酚。
3.根据权利要求1所述的方法，其特征在于，所述缓冲溶液为乙酸-乙酸钠缓冲液。
4.根据权利要求3所述的方法，其特征在于，所述乙酸-乙酸钠缓冲溶液浓度为0.1-0.3mol/L；pH为4.0-6.0。
5.根据权利要求1所述的方法，其特征在于，所述混合体系中漆酶浓度为25～125U/mL。
6.根据权利要求1所述的方法，其特征在于，所述混合体系中酚类化合物浓度为0.04-0.20mol/L。
7.根据权利要求1所述的方法，其特征在于，按照浴比为1：20-1：50，将羊毛纤维加入到混合体系中。
8.根据权利要求1-7任一项所述的方法，其特征在于，所述反应是在30-80℃保温反应2～10小时。
9.权利要求1-8任一项所述方法制备得到的羊毛纤维。
10.含有权利要求9所述的羊毛纤维的纱、线或者织物。
CN202011510926.7A 2020-12-18 2020-12-18 一种提高羊毛纤维强力的生物酶促染色方法 Active CN112647317B (zh)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN202011510926.7A CN112647317B (zh) 2020-12-18 2020-12-18 一种提高羊毛纤维强力的生物酶促染色方法
PCT/CN2022/073958 WO2022127940A1 (zh) 2020-12-18 2022-01-26 一种提高羊毛纤维强力的生物酶促染色方法
US17/873,463 US20220356643A1 (en) 2020-12-18 2022-07-26 Method for Improving Strength and Dyeing of Wool Fibers

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202011510926.7A CN112647317B (zh) 2020-12-18 2020-12-18 一种提高羊毛纤维强力的生物酶促染色方法

Publications (2)

Publication Number Publication Date
CN112647317A true CN112647317A (zh) 2021-04-13
CN112647317B CN112647317B (zh) 2021-12-28

Family

ID=75358360

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202011510926.7A Active CN112647317B (zh) 2020-12-18 2020-12-18 一种提高羊毛纤维强力的生物酶促染色方法

Country Status (3)

Country Link
US (1) US20220356643A1 (zh)
CN (1) CN112647317B (zh)
WO (1) WO2022127940A1 (zh)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022127940A1 (zh) * 2020-12-18 2022-06-23 江南大学 一种提高羊毛纤维强力的生物酶促染色方法
CN116411465A (zh) * 2021-12-30 2023-07-11 江苏欣鑫纺织科技有限公司 一种生物技术的蛋白纤维原位同浴加工方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105951436A (zh) * 2016-05-18 2016-09-21 江南大学 一种漆酶催化羊毛织物接枝β-环糊精的抗菌整理方法
CN107059395A (zh) * 2017-05-16 2017-08-18 无锡协新毛纺织股份有限公司 一种漆酶催化羊毛织物接枝含酚羟基壳聚糖的整理方法
CN107904935A (zh) * 2017-11-28 2018-04-13 苏州大学 疏水蚕丝织物及其制备方法和应用
CN111945425A (zh) * 2020-07-16 2020-11-17 江苏华佳丝绸股份有限公司 一种强疏水真丝织物及其制备方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6805718B2 (en) * 1995-12-22 2004-10-19 Novozymes A/S Enzymatic method for textile dyeing
CN102561053A (zh) * 2012-02-21 2012-07-11 苏州大学 一种漆酶催化茶多酚用于蚕丝染色的方法
CN104032604A (zh) * 2014-06-09 2014-09-10 江南大学 一种漆酶对羊毛织物的生物染色工艺
CN105780533A (zh) * 2016-03-11 2016-07-20 南通大学 一种含酶促茶叶色素的纺织品的染色方法
CN112647317B (zh) * 2020-12-18 2021-12-28 江南大学 一种提高羊毛纤维强力的生物酶促染色方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105951436A (zh) * 2016-05-18 2016-09-21 江南大学 一种漆酶催化羊毛织物接枝β-环糊精的抗菌整理方法
CN107059395A (zh) * 2017-05-16 2017-08-18 无锡协新毛纺织股份有限公司 一种漆酶催化羊毛织物接枝含酚羟基壳聚糖的整理方法
CN107904935A (zh) * 2017-11-28 2018-04-13 苏州大学 疏水蚕丝织物及其制备方法和应用
CN111945425A (zh) * 2020-07-16 2020-11-17 江苏华佳丝绸股份有限公司 一种强疏水真丝织物及其制备方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
白茹冰: "漆酶催化芳香化合物聚合及其对羊毛和棉织物染色研究", 《中国博士学位论文全文数据库 工程科技I辑》 *
袁萌莉等: "羊毛织物的漆酶催化没食子酸原位染色与改性", 《印染》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022127940A1 (zh) * 2020-12-18 2022-06-23 江南大学 一种提高羊毛纤维强力的生物酶促染色方法
CN116411465A (zh) * 2021-12-30 2023-07-11 江苏欣鑫纺织科技有限公司 一种生物技术的蛋白纤维原位同浴加工方法

Also Published As

Publication number Publication date
CN112647317B (zh) 2021-12-28
WO2022127940A1 (zh) 2022-06-23
US20220356643A1 (en) 2022-11-10

Similar Documents

Publication Publication Date Title
CN112647317B (zh) 一种提高羊毛纤维强力的生物酶促染色方法
Csiszár et al. Enzymes and chelating agent in cotton pretreatment
CN110344262B (zh) 一种涤纶织物染色与防水整理同浴一步加工方法
US10711392B2 (en) Pad-steam bleaching method for fabric based on TBLC-activated hydrogen peroxide system
CN104264490A (zh) 一种酶法羊毛染色的方法
Ibrahim et al. Novel pre-treatment processes to promote linen-containing fabrics properties
CN110952346A (zh) 一种适用于儿童服饰面料的涤纶染色添加剂
CN111287006B (zh) 一种羊毛低温弱损伤染色工艺
CN111910446A (zh) 一种涤棉混纺纱线染色工艺
Hebeish et al. Improving easy care nonformaldehyde finishing performance using polycarboxylic acids via precationization of cotton fabric
US10202723B2 (en) Method of treating polyester textile
Zolriasatein et al. Changes in composition, appearance, physical, and dyeing properties of jute yarn after bio-pretreatment with laccase, xylanase, cellulase, and pectinase enzymes
Ali et al. Cellulase enzyme in bio-finishing of cotton-based fabrics: effects of process parameters
CN110904699A (zh) 一种适用于儿童服饰面料的涤纶纱线染色方法
CN110004722A (zh) 一种多巴胺和疏水化合物共聚的疏水棉织物的制备方法
CN115354509A (zh) 一种棉织物漂白染色工艺
CN112813707B (zh) 一种基于漆酶/多酸协效反应的生态棉织物染色方法
Gunasekar et al. Eco-friendly textile dyeing processes
Rousselle et al. Effect of purified Trichoderma reesei cellulases on the supramolecular structure of cotton cellulose
Kan et al. The effect of cellulase treatment on hydrolysis of linen
CN117026652A (zh) 一种织物染色方法
Bendak et al. Pretreatment of Proteinic and Synthetic Fibres Prior to Dyeing
CN1732300A (zh) 纤维素本色织物的处理方法、由此方法获得的产品及其用途
Arumugam et al. Pretreatment of bleached cotton fibers with whole and monocomponent cellulases for nonwoven applications
Kenji et al. Saccharification and Ethanol Fermentation for Bioethanol Production from Alkali Pretreated Cotton Available for Practical Equipment

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant