CN112640972A - Preparation method of high-concentration theanine tea - Google Patents
Preparation method of high-concentration theanine tea Download PDFInfo
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- CN112640972A CN112640972A CN201910965990.5A CN201910965990A CN112640972A CN 112640972 A CN112640972 A CN 112640972A CN 201910965990 A CN201910965990 A CN 201910965990A CN 112640972 A CN112640972 A CN 112640972A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
- A23F3/14—Tea preparations, e.g. using additives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/18—Extraction of water soluble tea constituents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C231/00—Preparation of carboxylic acid amides
- C07C231/22—Separation; Purification; Stabilisation; Use of additives
- C07C231/24—Separation; Purification
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- Tea And Coffee (AREA)
Abstract
The invention discloses a preparation method of tea with high-concentration theanine, which mainly comprises the steps of extracting the theanine in the tea and reversely adding the theanine. Regarding the reverse adding step, the invention reversely adds the theanine powder which is extracted from the tea and has high concentration in the tea making process, so that the content of the theanine in the added tea is improved, and the content is accurate and controllable; the tea can keep taste when drinking, and the final product can supplement theanine required by human body.
Description
Technical Field
The invention relates to the technical field of tea, in particular to a preparation method of high-concentration theanine tea.
Background
Theanine is a free amino acid peculiar to tea leaves, and is glutamic acid gamma-ethylamide, and has sweet taste. Theanine content varies depending on the variety and the site of tea. Theanine accounts for 1-2% of the weight of the dry tea.
Theanine can affect metabolism and release of nerve transmission substances such as dopamine in brain, and has good adjuvant therapy effect on depression state of human body; the learning ability and the memory can be improved, the body and mind effects can be relaxed and happy, and the overexcitation caused by the caffeine can be reduced; can improve menstrual period syndrome, has protective effect on nerve cells, and can be used for prevention and adjuvant treatment of senile dementia; theanine reduces blood pressure by regulating the concentration of nerve transmission substances in brain, and has no blood pressure reducing effect on normal blood pressure; the theanine can be used together with anticancer drugs to improve the anticancer effect of the drugs.
At present, high-concentration theanine tea taking tea leaves as a carrier is still a blank in the research aspect of tea leaves, and compared with other foods added with theanine, the tea leaves with high-concentration theanine have the advantages that the active ingredients are easier to absorb and utilize by human bodies.
Therefore, it is an urgent problem for those skilled in the art to provide a method for preparing tea leaves with high concentration of theanine by utilizing the properties of theanine.
Disclosure of Invention
In view of this, the present invention provides a method for preparing tea leaves with high theanine concentration.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing tea with high concentration of theanine comprises extracting theanine from tea and adding theanine.
By adopting the technical scheme, the invention has the following beneficial effects:
the domestic tea research institution purifies solid granules or powder of theanine from tea. However, the batch and accurate dose production of finished tea products is not available at home and abroad from the initial research on theanine in tea to the present, and the key points are three:
firstly, the taste of the tea leaves is changed due to technical problems, so that the tea leaves are difficult to drink normally;
secondly, the theanine tea is prepared each time, and the amount of the theanine generated each time is unstable due to high variability of temperature, environment, metering of used auxiliary materials and concentration;
and thirdly, because the processing and production processes are multiple and the requirements are strict, the mass production is almost impossible.
The invention utilizes the reverse addition technology to extract the theanine from the tea firstly, and then adds the theanine into the tea by corresponding technical means, thereby ensuring the original theanine amount of the tea and adding the theanine again at the same time, and being capable of obviously improving the concentration of the theanine in the tea.
Further, the extraction steps of the theanine in the tea leaves are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 90-100 deg.C with a ratio of materials to liquid of 20g/ml, filtering residue, extracting repeatedly for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20 k-pore ultrafiltration membrane at 30-35 deg.C and operating pressure of 0.25-0.3MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
Further, the leaching time in the leaching process in the step (1) is 40-50 min.
Further, the reverse addition step of theanine is specifically as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering theanine crystalline solid particles or powder prepared in the step (3) on the opened tea leaves in proportion;
and S2, carrying out subsequent white tea processing.
Further, in the step S1, the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 250-300: 1.
Further, in the step S2, the processing temperature of the white tea is less than or equal to 200 ℃.
By adopting the technical scheme, the invention has the following beneficial effects:
the tea has strong adsorption function, and the theanine is a self substance and is very easy to dissolve in water, so that the operation of uniformly adding the theanine in the tea is simple, and the process can be completed in a plurality of steps for making the tea. The theanine is added in the sun-drying process, and aims to increase the content of theanine in the finished tea; and the added theanine is more fully fused with the tea, so that the good taste of the finished tea can be ensured.
The daily intake of theanine is recommended by countries not to exceed 400mg, if the added theanine is taken by human body in the form of tablets, puffed food and the like, the problem of overdose is easy to occur, and the excessive dose of the theanine can cause the risk of addiction and the like. According to the technical scheme, the tea is reversely added, so that the content of theanine in the tea is obviously improved, a proper amount of theanine can be supplemented for a human body through stable and batch production, the hazards of addiction and the like are avoided, the preparation process is simple, and the method is suitable for further popularization and production.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
example 1 discloses a method for preparing tea leaves with high theanine concentration, comprising a step of extracting theanine from tea leaves and a step of adding theanine reversely.
Wherein, the extraction steps of the theanine in the tea are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 100 deg.C for 50min at a material-to-liquid ratio of 20g/ml, filtering residue, extracting for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20k ultrafiltration membrane at 35 deg.C and 0.3MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
The steps of the reverse addition of theanine are as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering prepared theanine crystal type solid particles or powder on the opened tea leaves according to a ratio, wherein the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 300: 1, and the white tea raw material is 1000 g.
S2, carrying out subsequent white tea processing, wherein the process temperature of the white tea processing is less than or equal to 200 ℃.
Example 2:
example 2 discloses a method for preparing tea leaves with high theanine concentration, which comprises a step of extracting theanine from tea leaves and a step of adding theanine reversely.
Wherein, the extraction steps of the theanine in the tea are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 90 deg.C for 40min at a material-to-liquid ratio of 20g/ml, filtering residue, extracting for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20k ultrafiltration membrane at 30 deg.C and 0.25MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
The steps of the reverse addition of theanine are as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering prepared theanine crystal type solid particles or powder on the opened tea leaves according to a ratio, wherein the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 250: 1, and the white tea raw material is 1000 g.
S2, carrying out subsequent white tea processing, wherein the process temperature of the white tea processing is less than or equal to 200 ℃.
Example 3:
example 3 discloses a method for preparing tea leaves with high theanine concentration, comprising a step of extracting theanine from tea leaves and a step of adding theanine reversely.
Wherein, the extraction steps of the theanine in the tea are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 95 deg.C for 48min at a material-to-liquid ratio of 20g/ml, filtering residue, extracting for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20 k-pore ultrafiltration membrane at 33 deg.C and 0.27MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
The steps of the reverse addition of theanine are as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering prepared theanine crystal type solid particles or powder on the opened tea leaves according to a ratio, wherein the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 280: 1, and the white tea raw material is 1000 g.
S2, carrying out subsequent white tea processing, wherein the process temperature of the white tea processing is less than or equal to 200 ℃.
Example 4:
example 4 discloses a method for preparing tea leaves with high theanine concentration, comprising a step of extracting theanine from tea leaves and a step of adding theanine reversely.
Wherein, the extraction steps of the theanine in the tea are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 98 deg.C for 42min at a material-to-liquid ratio of 20g/ml, filtering residue, extracting for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20 k-pore ultrafiltration membrane at 34 deg.C and 0.29MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
The steps of the reverse addition of theanine are as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering prepared theanine crystal type solid particles or powder on the opened tea leaves according to a ratio, wherein the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 270: 1, and the white tea raw material is 1000 g.
S2, carrying out subsequent white tea processing, wherein the process temperature of the white tea processing is less than or equal to 200 ℃.
Example 5:
example 5 discloses a method for preparing tea leaves with high theanine concentration, which comprises a step of extracting theanine from tea leaves and a step of adding theanine reversely.
Wherein, the extraction steps of the theanine in the tea are as follows:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 93 deg.C for 45min at a material-to-liquid ratio of 20g/ml, filtering residue, extracting for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20k ultrafiltration membrane at 32 deg.C and 0.28MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
The steps of the reverse addition of theanine are as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering prepared theanine crystal type solid particles or powder on the opened tea leaves according to a ratio, wherein the ratio of the white tea raw material to the theanine crystal type solid particles or powder is 260: 1, and the white tea raw material is 1000 g.
S2, carrying out subsequent white tea processing, wherein the process temperature of the white tea processing is less than or equal to 200 ℃.
In order to further demonstrate the technical effects of the present invention, the applicant conducted tests on the high-concentration theanine white tea prepared in examples 1 and 2 and the raw white tea without theanine (comparative example 1). The detection report of this detection is issued by the national tea quality supervision and inspection center, and the serial number is as follows: no. 2019W-2071-2082.
The detection mode is as follows:
the white tea raw tea obtained in example 1 and example 2 was brewed separately from the white tea raw tea to which no theanine was added.
The brewing method comprises the following steps:
reference is made to GB/T23776-.
Respectively taking 3g of tea samples, soaking for 5min by using 150mL of boiling water, and filtering to obtain a first soaking test sample:
then, the tea leaves are brewed for 5min by 150mL of boiling water, and the operations are repeated to respectively obtain a second soaking sample and a third soaking sample.
The theanine content of the sample is detected, and the specific results are as follows:
see table 1 for results of comparative example 1:
TABLE 1
Sample name | Name of item | The result of the detection |
White tea raw material | Theanine | 0.68% |
White tea raw material (first brewing) | Theanine | 8.16mg |
White tea raw material (second brewing) | Theanine | 6.33mg |
White tea raw material (third time brewing) | Theanine | 4.77mg |
See table 2 for the results of example 1:
TABLE 2
Sample name | Name of item | The result of the detection |
White tea test sample | Theanine | 2.22% |
White tea test sample (first brewing) | Theanine | 40.0mg |
White tea test sample (second brewing) | Theanine | 11.2mg |
White tea test sample (third time brewing) | Theanine | 5.85mg |
See table 3 for the results of example 2:
TABLE 3
Sample name | Name of item | The result of the detection |
White tea test sample | Theanine | 1.32% |
White tea test sample (first brewing) | Theanine | 24.3mg |
White tea test sample (second brewing) | Theanine | 8.31mg |
White tea test sample (third time brewing) | Theanine | 5.37mg |
As is apparent from the comparison, the content of theanine is 3.26 times that of the comparative example, the concentration is remarkably improved, and the concentration is in a range suitable for human bodies.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A preparation method of tea with high-concentration theanine is characterized by comprising the steps of extracting the theanine in the tea and reversely adding the theanine.
2. The method for preparing tea leaves with high concentration of theanine according to claim 1, wherein the extraction of theanine from tea leaves comprises the steps of:
(1) pulverizing and extracting
Pulverizing folium Camelliae sinensis, extracting with deionized water at 90-100 deg.C with a ratio of materials to liquid of 20g/ml, filtering residue, extracting repeatedly for 2 times, mixing filtrates, concentrating, precipitating with 95% ethanol solution, filtering, concentrating to remove ethanol to obtain extractive solution;
(2) ultra-filtration treatment
Passing the extractive solution through 20 k-pore ultrafiltration membrane at 30-35 deg.C and operating pressure of 0.25-0.3MPa, and collecting the filtrate;
(3) purification of
Concentrating the permeate, then carrying out elution on the concentrated permeate through a pretreated PA resin adsorption column by adopting 70% ethanol aqueous solution at the flow rate of 1 BV/h; separating with NKA-II resin-filled chromatographic column, eluting with distilled water at flow rate of 1BV/h, concentrating the eluate of 1.5-2.5BV, sampling again, and drying to obtain theanine powder.
3. The method for preparing tea leaves with high concentration of theanine as claimed in claim 2, wherein the leaching time in the leaching process in the step (1) is 40-50 min.
4. The method for preparing tea leaves with high theanine concentration according to claim 3, wherein the reverse addition step of theanine is as follows:
s1, opening a sun-dried white tea raw material, and uniformly scattering theanine crystalline solid particles or powder prepared in the step (3) on the opened tea leaves in proportion;
and S2, carrying out subsequent white tea processing.
5. The method as claimed in claim 4, wherein the ratio of the white tea material to the crystalline solid granules or powders of theanine in the step S1 is 300: 1 to 250-.
6. The method for preparing tea leaves with high theanine concentration as claimed in claim 4, wherein the processing temperature of the white tea is 200 ℃ or less in the step S2.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113768004A (en) * | 2021-08-18 | 2021-12-10 | 福鼎市三余茶业有限公司 | White tea seasoning tea containing dried orange peel and preparation method thereof |
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CN102127048A (en) * | 2010-12-28 | 2011-07-20 | 南京农业大学 | Method for preparing tea polyphenol and theanine from summer and autumn green tea |
CN104083532A (en) * | 2014-07-18 | 2014-10-08 | 黄山华绿园生物科技有限公司 | Method for extracting tea pigment and L-theanine from tea leaves |
CN107473979A (en) * | 2017-08-20 | 2017-12-15 | 合肥信达膜科技有限公司 | A kind of processing method of film extraction theanine |
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US20070248737A1 (en) * | 2006-03-03 | 2007-10-25 | Conopco Inc, D/B/A Unilever | Process for preparing a tea product |
CN101801205A (en) * | 2007-09-19 | 2010-08-11 | 荷兰联合利华有限公司 | Beverage precursor and process for the manufacture thereof |
CN102127048A (en) * | 2010-12-28 | 2011-07-20 | 南京农业大学 | Method for preparing tea polyphenol and theanine from summer and autumn green tea |
CN104083532A (en) * | 2014-07-18 | 2014-10-08 | 黄山华绿园生物科技有限公司 | Method for extracting tea pigment and L-theanine from tea leaves |
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