CN112640896A - Surface-modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof - Google Patents

Surface-modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof Download PDF

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CN112640896A
CN112640896A CN202011557926.2A CN202011557926A CN112640896A CN 112640896 A CN112640896 A CN 112640896A CN 202011557926 A CN202011557926 A CN 202011557926A CN 112640896 A CN112640896 A CN 112640896A
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modified silver
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吴学民
商洪溢
徐勇
孙喆
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China Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Engineering & Computer Science (AREA)
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  • Inorganic Chemistry (AREA)
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Abstract

The invention discloses a surface modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof. The surface modified silver nano suspending agent comprises the following components in percentage by mass: 0.02-1% of silver nitrate; 0.2-5% of surface functional modifier; 0.1-5% of a dispersant; 0.1-5% of an antifreezing agent; 0.1-2% of a thickening agent; the balance of water. The invention takes water as a medium, and is green and environment-friendly. The ultraviolet rays in natural sunlight are used as reducing conditions, and the preparation method is green and simple. The prepared silver nanometer suspending agent has the particle size of 10-100 nm, can efficiently penetrate through the highly lignified epidermis of pine trees through the nanometer particle size and the surface modification of the lipophilic penetration conductive agent, is conducted in the pine bodies, has good killing effect on the pine wood nematodes, and realizes the effective treatment on the pine wood nematode diseases.

Description

Surface-modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof
Technical Field
The invention relates to a surface modified silver nano suspending agent for preventing and treating pine wood nematode disease and application thereof, belonging to the technical field of pesticides.
Background
In 2018, in 2 months, the national forest area reaches 31.2 hundred million mu, the forest coverage rate reaches 21.66%, and the forest accumulation amount reaches 151.37 billion cubic meters, including forest resources such as northeast Xingan mountains, lesser Xingan mountains, northwest forest zones, southern forest zones and the like. But the defects in the forestry production are increasingly highlighted, according to the latest statistics, the species of Chinese forestry pests reach 6201, the area of the pests breaks through 1.8 hundred million mu in two years in the last year, 4000 tens of thousands of plants kill the trees, and the indirect economic loss is 856 million yuan. The bursaphelenchus xylophilus is called pine cancer, belongs to a national serious ecological disaster, is the most dangerous and destructive disease in a global forest ecological system, has extremely strong diffusivity and destructiveness, poses a serious threat to the safety of pine forest of nearly 9 hundred million acres, and is one of very serious forest diseases.
At present, the prevention and control of the pine wilt disease only focuses on the unmanned aerial vehicle pesticide application prevention and control of a transmission medium pine beetle (spruce flower black beetle in Liaoning area), and the prevention and control means only has about 70 percent of prevention and control effect on the transmission medium at present, so that the transmission of the pine wilt disease can be only reduced locally, and the origin of the pine wilt disease cannot be really cut off. The varieties of active ingredients registered in China for preventing and controlling the pine wood nematodes are only 3, the preparation products are only 10, the action modes are punching and medicine injection, only pine wood nematode disease can be prevented, the pine wood nematode disease cannot be effectively treated, and at present, no active ingredient can be directly sprayed on disease-sensitive pine trees to kill the nematodes in the trees.
The pesticide has slow development in forestry, and is mainly limited in the following aspects: firstly, pine has thick surface and serious lignification, and the liquid medicine is difficult to permeate. In addition, the secretion of the grease of the Pinaceae plants is vigorous, and the conduction of the effective components in the tree body is limited; the liquid medicine is difficult to retain on the pine needles, etc.
The development of the nanotechnology brings new opportunities to the industries of pesticide, feed, food and the like. According to statistics, the research and application fields of the nano-silver mainly focus on medical bactericides, food contact materials, feed additives, food additives and the like, and the application in the pesticide field is rarely reported. The nano-drug has the advantages of high surface activity, high permeability, high adhesiveness and the like, and has good application prospect. It is estimated that 320 ten thousand tons of nano silver particles are manufactured every year for medical, biosensing and food applications. Therefore, it is necessary to provide a nano-silver medicament for preventing and treating the pine wilt disease.
Disclosure of Invention
The invention aims to provide a surface modified silver nano suspending agent and application thereof in preventing and treating pine wood nematode; the surface-modified silver nano suspending agent is prepared by adopting a green sunlight reduction method, has concentrated particle size, a unique pine wood nematode killing mechanism and higher pine wood nematode killing capability, and has higher prevention and control effect on pine wood nematode diseases; the invention makes the silver nanometer suspending agent more compatible with a rosin system through the surface modification of the lipophilic modified penetration conduction auxiliary agent, has strong penetration capacity to the surface of pine trees and has good conduction capacity in the pine trees.
The surface modified silver nano suspending agent for preventing and treating the pine wood nematode disease provided by the invention comprises the following components in percentage by mass:
Figure BDA0002859329410000021
the balance of water.
Specifically, the mass percentage of the silver nitrate is preferably 0.05-1%;
the particle size of the silver nanoparticles in the surface modified silver nano suspending agent is 10-100 nm.
Specifically, the surface functional modifier can be fatty alcohol-polyoxyethylene ether (penetrating agent JFC) or siloxane-polyoxyethylene ether (Silwet-625);
the mass percentage of the surface functional modifier is preferably 0.5-3%, and more preferably 0.5-2%;
in the surface-modified silver nano suspending agent, the surface functional modifier plays two roles as follows: the silver nanoparticle is provided with a soft template generated by the silver nanoparticles through a space effect and a charge effect, and has higher pine affinity capacity through surface modification of the silver nanoparticles, so that the permeation and conduction effects are enhanced.
Specifically, the dispersant may be sodium lignosulfonate, an alkylnaphthalene sulfonate formaldehyde polycondensate, a carboxylate high molecular polymer, an EO-PO block copolymer, Morwet D-425 or Terspense-2500;
the mass percentage content of the dispersant is preferably 1-2%, and more preferably 0.5-1%.
Specifically, the antifreezing agent can be short-chain alcohol with 1-3 carbon atoms;
the short-chain alcohol can be ethylene glycol, glycerol or polyethylene glycol;
the mass percentage of the anti-freezing agent is preferably 0.2-2%, and more preferably 2%.
Specifically, the thickener may be xanthan gum or magnesium aluminum silicate;
the mass percentage of the thickener is preferably 0.1-1%, and more preferably 0.1-0.2%.
Specifically, the water is preferably deionized water or purified water.
The surface-modified silver nano suspending agent preferably comprises the following components:
1) 0.05-1% of silver nitrate; 0.2-2% of surface functional modifier; 0.5-1% of a dispersant; 2% of an antifreezing agent; 0.1-0.2% of a thickening agent; the balance of water;
2) 0.05% of silver nitrate; 2% of surface functional modifier; 0.5 percent of dispersant; 2% of an antifreezing agent; 0.1% of thickening agent; the balance of water;
3) 0.2% of silver nitrate; 2% of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.1% of thickening agent; the balance of water;
4) 0.5 percent of silver nitrate; 2% of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.15% of thickening agent; the balance of water;
5) 1% of silver nitrate; 2% of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.15% of thickening agent; the balance of water;
6) 0.05% of silver nitrate; 0.5 percent of surface functional modifier; 0.5 percent of dispersant; 2% of an antifreezing agent; 0.1% of thickening agent; the balance of water;
7) 0.2% of silver nitrate; 1% of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.1% of thickening agent; the balance of water;
8) 0.5 percent of silver nitrate; 1.5 percent of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.1% of thickening agent; the balance of water;
9) 1% of silver nitrate; 2% of surface functional modifier; 1% of a dispersant; 2% of an antifreezing agent; 0.2% of thickening agent; the balance of water.
The invention also provides a preparation method of the surface modified silver nano suspending agent, which comprises the following steps:
and preparing the aqueous solution of silver nitrate, then dropwise adding the aqueous solution of the surface functional modifier, the dispersant, the antifreezing agent and the thickener, stirring the mixture at room temperature for 8-10 hours under the irradiation of sunlight, changing the color of the solution from light to dark, and continuously stirring the mixture for 2-6 hours to obtain the silver nitrate nano-particles.
The surface-modified silver nano suspending agent provided by the invention can be used for preventing and treating the pine wilt disease, the silver nano suspending agent diluent (the dilution multiple is 50-500 times) is sprayed on the gummy pine (in the disease-sensitive initial stage) and the discolored wood (the discoloration proportion is less than 30%) in the outbreak peak period of the pine wilt disease, and unmanned aerial vehicle pesticide application or spray rod sprayers are selected for spraying according to the height of the pine and the terrain factor.
The invention has the following beneficial effects:
1. silver is used as an effective component, has a unique killing mechanism and high-efficiency killing capability on the pine wood nematodes, and is not easy to generate drug resistance.
2. The surface modification is carried out on the silver particles by the lipophilic modified permeable conductive agent, so that the silver particles have high permeability and conductivity.
3. The first report is that the agent can be directly sprayed on the susceptible pine tree to pull out the origin of the disease.
4. The preparation method is green, simple, convenient and environment-friendly, and is easy for mass production.
5. Good physical stability and difficult agglomeration.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 preparation of novel silver nanosuspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000041
the preparation method comprises the following steps:
(1) silver nitrate (1 g in hundred) was weighed and dissolved in 40g of water.
(2) 2g (folding hundred) fatty alcohol-polyoxyethylene ether, 1g (folding hundred) D-425, 2g (folding hundred) glycol and 0.2g xanthan gum are weighed and dissolved in 50g of aqueous solution, and the mixture is continuously stirred.
(3) And (3) slowly dropwise adding the solution in the step (1) into the solution in the step (2), and stirring for 8 hours at room temperature in sunlight.
(4) The solution changed from light to dark at this time and was stirred for 6 h.
Example 2 preparation of a novel silver nanosuspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000042
the preparation was carried out as in example 1.
Example 3 preparation of a novel silver nanosuspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000043
the preparation was carried out as in example 1.
Example 4 preparation of a novel silver nanosuspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000051
the preparation was carried out as in example 1.
Example 5 preparation of novel nanosilver suspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000052
the preparation was carried out as in example 1.
Example 6 preparation of novel nanosilver suspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000053
the preparation was carried out as in example 1.
Example 7 preparation of novel nanosilver suspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000054
Figure BDA0002859329410000061
the preparation was carried out as in example 1.
Example 8 preparation of novel nanosilver suspension
The weight percentage of each component is as follows:
Figure BDA0002859329410000062
the preparation was carried out as in example 1.
Comparative example 1 preparation method of novel silver nano-aqueous suspension
The chemical synthesis method in the literature is selected to compare the physicochemical properties of the silver nano suspending agent prepared by the method, and Dynamic Light Scattering (DLS) is adopted to evaluate the particle size and the distribution of the prepared silver nano particles.
The suspending agent prepared in example 8 was compared with a sample (hereinafter referred to as "Fig 3 f") of Fig3f in the references of Size-controlled silver synthesized over the range 5 to 100nm using the same product and the anti-microbial effect, and it was found that the particle sizes were similar as shown in Table 1. The particle size distribution is measured by the particle size dispersity index (PDI).
TABLE 1 comparison of particle size and Dispersion of silver nanosuspensions
Test sample Particle size (nm) PDI
Example 8 28 0.326
Fig3f 30 0.559
The data in Table 1 show that the chemically prepared silver nanoparticles in the literatureThe particle PDI was 0.559, which is greater than the particles prepared by the process of the present invention (PDI 0.326), with larger values indicating more discrete surface distributions and smaller values indicating more concentrated distributions. The invention adopts a photoreduction method, and can slowly reduce Ag+The Ag is simple substance, and compared with the chemical method, the whole system has mild reduction process and inhibits the austenite curing phenomenon.
Example 9 evaluation of stability of surface-modified silver nanosuspension
The nano-silver suspensions prepared in examples 1-8 were subjected to a stability test at 54 ± 2 ℃ for 14 days.
The change in silver content was measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES), and the results are shown in Table 2.
The particle size change of the nano silver particles before and after the stability test was measured by Dynamic Light Scattering (DLS) analysis, and the detection results are shown in table 3.
Table 2 evaluation results of silver content stability of surface-modified silver nanosuspension (14 days)
Serial number Content (%) before nano-silver storage Content (%) e of nano silver after storage
Example 1 1.05 1.05
Example 2 0.50 0.50
Example 3 0.21 0.20
Example 4 0.05 0.05
Example 5 1.04 1.04
Example 6 0.51 0.51
Example 7 0.22 0.22
Example 8 0.05 0.05
Table 3 evaluation results of particle size stability of surface-modified silver nanosuspension (14 days)
Serial number Nano silver particle size D before storage50(nm) Grain diameter D of nano silver after storage50(nm)
Example 1 70.55 74.29
Example 2 50.58 66.08
Example 3 56.10 59.25
Example 4 18.26 18.65
Example 5 75.39 80.20
Example 6 60.02 68.13
Example 7 48.77 50.67
Example 8 28.35 29.99
From the storage stability results, the content and the particle size of the silver nano suspending agent prepared by the invention have no obvious change, which shows that the stability is good.
EXAMPLE 10 controlling Effect of surface-modified silver Nanosuspenants
1. Indoor bioassay evaluation of silver nanosuspensions
Test agents: 1% silver nanosuspension prepared in example 1
Control agents: 3% emamectin benzoate microemulsion
Test subjects: pine wood nematode (Queen Wahai city Kunjia infected pine wood, Shandong province)
The test method comprises the following steps: the indoor efficacy of the novel surface modified silver nano suspending agent is evaluated by using a common method for nematode efficacy experiments.
Firstly, culturing test nematodes in agar culture medium full of botrytis cinerea, washing and collecting the nematodes with deionized water after the nematodes grow until the botrytis cinerea is eaten, and diluting the nematodes to 1000 heads/ml by using a microscope counting method. Performing 1: 1 mixing and setting blank processing. Standing at room temperature for 12h, performing microscopic examination with microscope, counting dead nematodes, and performing drug effect by LC50And (4) showing.
And (3) test results: as shown in Table 4, the silver nano suspending agent and the emamectin benzoate microemulsion have killing effects on the pine wood nematodes, and the silver nano suspending agent is used as a novel nematicidal effective component, and has indoor toxicity equivalent to that of the current common emamectin benzoate.
TABLE 4 indoor toxicity test results of the surface-modified silver nano-suspending agent and emamectin benzoate microemulsion on pine wood nematodes
Figure BDA0002859329410000081
2. Evaluation of efficacy of pine wood nematode inoculated pine tree with silver nano suspending agent
Test agents: 1% silver nanosuspension prepared in example 1
Control agents: 3% emamectin benzoate microemulsion and 5% abamectin oil emulsion
And (3) testing pine trees: black pine, about 5 years old
The control object is: pine wood nematode (Queen Wahai city Kunjia infected pine wood, Shandong province)
Test site: china forest farm of double islands in the ring green region of Weihai city, Shandong province
The test method comprises the following steps: the pine tree inoculation method is used for evaluating the treatment effect of the silver nano suspending agent and the emamectin benzoate microemulsion on pine trees suffering from bursaphelenchus xylophilus infection.
Washing the nematodes cultured in the culture medium with clear water, taking out, adjusting the concentration of the nematodes to 2000 heads/ml by microscopic examination, cutting the bark of the pine tree, adsorbing 2ml of the diluent of the nematodes with absorbent cotton, inserting the diluent into a cut, and fixing the absorbent cotton with a sealing film. And spraying the pine trees with different concentrations of silver nano suspending agent, emamectin benzoate microemulsion and abamectin emulsifiable concentrate 7 days after inoculation, wherein 10 trees in each group are sprayed, and a control is set.
Test investigation: the disease incidence of each group is counted 7 days after the control group is attacked, and the disease incidence is expressed by the number of diseased plants.
And (3) test results: as shown in Table 5, the silver nano suspending agent has a remarkable treatment effect on pine tree inoculated by the pine wood nematodes, and the nano-scale particles and the surface modification coating of the penetrant have a stronger permeation and conduction effect on the pine trees. The common emamectin benzoate microemulsion and the avermectin emulsifiable concentrate cannot penetrate through the epidermis of the pine tree, and although the stomata of the pine needle can absorb part of effective components, the effective components cannot be conducted in branches, so that the common emamectin benzoate microemulsion and the avermectin emulsifiable concentrate cannot effectively kill the pine wood nematodes in the tree body.
TABLE 5 prevention and treatment effect of silver nano suspending agent on rice bacterial brown streak
Figure BDA0002859329410000091
EXAMPLE 12 comparison of Permeability of novel silver nanosuspensions
The penetration performance of pine branches is compared by selecting silver nano particles with other surface modifications in the literature and the silver nano water suspending agent, and the penetration depth is analyzed by adopting a confocal surface enhanced Raman microscope.
The suspending agent prepared in example 4 was selected to compare with the sodium Alginate-Coated Silver nanoparticles in FIG. 1h (hereinafter referred to as FIG. 1h) prepared in Green Synthesis of an Alginate-Coated Silver nanoparticles High-Activity by Enhancing Its Ability to Membrane nanoparticles adsorption, and the particle sizes of the two were close and the surface-modified compounds were different. 4-mercaptobenzoic acid was used as a contrast agent for Raman confocal microscopy to evaluate the penetration depth of both on pine branches after 12 h.
TABLE 6 comparison of penetration depth of silver nanosuspensions
Test sample Depth of penetration (micron)
Example 4 86.5
Fig1h 43.1
The data in Table 6 show that the silver nano aqueous suspension has strong permeability to pine branches, and the surface modification component has strong affinity to pine trees, so that the surface tension of the interface between the liquid medicine and the pine branches is reduced, and the silver nano aqueous suspension has good permeability. While the silver nanoparticles coated with sodium alginate in the literature have weak penetration capability to pine branches, the surface modification compound is a biological macromolecule, and the penetration capability to pine branches is weak.

Claims (10)

1. A surface modified silver nanometer suspending agent for preventing and treating pine wood nematode disease comprises the following components in percentage by mass:
Figure FDA0002859329400000011
the balance of water.
2. The surface-modified silver nanosuspension according to claim 1, wherein: in the surface modified silver nano suspending agent, the mass percentage of silver nitrate is 0.05-1%;
the particle size of the silver nanoparticles in the surface modified silver nano suspending agent is 10-100 nm.
3. The surface-modified silver nanosuspension according to claim 1 or 2, wherein: the surface functional modifier is fatty alcohol-polyoxyethylene ether or siloxane-polyoxyethylene ether;
the mass percentage of the surface functional modifier is 0.5-3%.
4. The surface-modified silver nanosuspension according to any one of claims 1 to 3, wherein: the dispersant is sodium lignosulfonate, alkyl naphthalene sulfonate formaldehyde polycondensate, carboxylate high-molecular polymer, EO-PO block copolymer, Morwet D-425 or Terspese-2500;
the mass percentage of the dispersant is 1-2%.
5. The surface-modified silver nanosuspension according to any one of claims 1 to 4, wherein: the anti-freezing agent is short-chain alcohol with 1-3 carbon atoms;
the short-chain alcohol is ethylene glycol, glycerol or polyethylene glycol;
the mass percentage of the anti-freezing agent is 0.2-2%.
6. The surface-modified silver nanosuspension according to any one of claims 1 to 5, wherein: the thickening agent is xanthan gum or magnesium aluminum silicate;
the mass percentage of the thickening agent is 0.1-1%.
7. The surface-modified silver nanosuspension according to any one of claims 1 to 6, wherein: the water is deionized water or purified water.
8. A process for preparing the surface-modified silver nanosuspension of any one of claims 1 to 7, comprising the steps of:
and preparing the aqueous solution of silver nitrate, then dropwise adding the aqueous solution of the surface functional modifier, the dispersant, the antifreezing agent and the thickener, stirring the mixture at room temperature for 8-10 hours under the irradiation of sunlight, changing the color of the solution from light to dark, and continuously stirring the mixture for 2-6 hours to obtain the silver nitrate nano-particles.
9. Use of the surface-modified silver nanosuspension according to any one of claims 1 to 7 for the control of pine wilt disease.
10. Use according to claim 9, characterized in that: spraying the diluent of the surface modified silver nano suspending agent to the gummy pine trees and the discolored wood in the peak period of the pine wood nematode disease;
the dilution multiple of the diluent is 50-500.
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CN111109295A (en) * 2020-01-16 2020-05-08 陕西麦可罗生物科技有限公司 Pesticide composition with kasugamycin and nano-silver as active components and application thereof

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