CN112626043A - Rumen-bypass glucose oxidase and preparation method and use method thereof - Google Patents
Rumen-bypass glucose oxidase and preparation method and use method thereof Download PDFInfo
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- CN112626043A CN112626043A CN202011612475.8A CN202011612475A CN112626043A CN 112626043 A CN112626043 A CN 112626043A CN 202011612475 A CN202011612475 A CN 202011612475A CN 112626043 A CN112626043 A CN 112626043A
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
- C12Y101/03—Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
- C12Y101/03004—Glucose oxidase (1.1.3.4)
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Abstract
The invention provides rumen-bypass glucose oxidase which comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase. The invention has the beneficial effects that: the feed additive contains green and safe components, has no drug residue, and can effectively improve the intestinal health of ruminant, and improve the milk yield and milk production performance of milk cow.
Description
Technical Field
The invention relates to the field of preparation of rumen bypass glucose oxidase, and particularly relates to rumen bypass glucose oxidase as well as a preparation method and a use method thereof.
Background
The glucose oxidase is aerobic dehydrogenase, and the beta-D-glucose is specifically oxidized into gluconic acid and H202While consuming oxygen. In the presence of catalase, hydrogen peroxide decomposes to produce water.
Glucose oxidase plays an important role in animal production. It can improve intestinal health of livestock and poultry, and improve immunity of organism; the feed additive can inhibit the growth of intestinal pathogenic bacteria, inhibit coccidian invasion, reduce intestinal diseases, improve the condition of livestock and poultry manure, obviously reduce the concentration of ammonia in livestock and poultry houses, and improve the environment of the livestock and poultry houses; can relieve the harm of mould and mycotoxin, maintain the health of livestock and poultry, and improve the production performance and comprehensive breeding benefit of livestock and poultry.
In ruminant application, on one hand, the glucose oxidase can improve the production performance of dairy cows, effectively relieve the common phenomenon of inappetence caused by perinatal period of the dairy cows, grass replacement of calves and diseases, and also has the effects of preventing retained afterbirth, reducing metabolic diseases, improving long-term dyspepsia of the dairy cows and the like. On the other hand, the glucose oxidase improves the quality of the silage, because the glucose oxidase can consume oxygen in the silage process, anaerobic lactobacillus proliferation is facilitated, the lactobacillus fermentation process is accelerated, the pH value of the silage is rapidly reduced, harmful bacterium reproduction is inhibited, abnormal fermentation is avoided, and the silage quality is finally ensured. Glucose oxidase is a protein in chemical nature, is easily degraded by rumen microorganisms when entering the rumen, and mostly loses the due functions.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides rumen-bypass glucose oxidase, a preparation method and a use method thereof.
The purpose of the invention is realized by the following technical scheme.
Rumen-bypass glucose oxidase comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase.
Preferably, the weight percentage of each component is as follows: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
A method for using rumen-bypass glucose oxidase, wherein the rumen-bypass glucose oxidase is uniformly mixed with a concentrate supplement according to the proportion of 100-200mg per kilogram of body weight per day for feeding.
A preparation method of rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting palm fat powder, and keeping the temperature to 55 ℃ for later use;
step two, taking glucose oxidase, lipase and silicon dioxide, uniformly mixing the glucose oxidase, the lipase and the silicon dioxide with the palm fat powder prepared in the step one, sieving the mixture by a sieve of 80 meshes, transferring the mixture into a centrifugal granulator, stirring the mixture, taking out the mixture after a certain time, and sieving the mixture by a sieve of 60 meshes;
and thirdly, taking the core particles with the particle size less than or equal to 60 meshes in the second step, putting the core particles into a centrifugal granulator again, and slowly adding calcium stearate and the palm fat powder prepared in the first step to prepare the rumen-bypass glucose oxidase.
Preferably, the weight percentage of the glucose oxidase added in the second step is 35-45%.
Preferably, according to any of the above schemes, the weight percentage of the glucose oxidase added in the second step is 40%.
Preferably, according to any of the above schemes, the weight percentage of the lipase added in the second step is 1% -3%.
Preferably, in any of the above embodiments, the weight percentage of the lipase added in the second step is 2%.
Preferably, according to any of the above schemes, the weight percentage of the silica added in the second step is 7% -9%.
Preferably, in any of the above embodiments, the weight percentage of the silica is 8%.
Preferably, in any of the above embodiments, the weight percentage of the palm fat powder added in the second step is 34%.
Preferably, according to any of the above schemes, the weight percentage of the calcium stearate added in the third step is 5% -7%.
Preferably, in any of the above embodiments, the weight percentage of the calcium stearate is 6%.
Preferably, in any of the above schemes, the weight percentage of the palm fat powder added in the third step is 10%.
The invention has the beneficial effects that:
the rumen bypass glucose oxidase product prepared by the method is yellow or light yellow granular material, mainly comprises glucose oxidase and protective material, and the content of the effective component of the product glucose oxidase is more than 40%. The feed is prepared by adopting the protection of fatty acid from plant sources through a coating technology, so that the product has high safety and no side effect, can be directly added into feed, and can also be used as a feed additive to be added into various ruminant feeds.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples.
Formula of rumen-bypass glucose oxidase
Rumen-bypass glucose oxidase comprises the following components in percentage by weight: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase.
Preferably, the weight percentage of each component is as follows: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
The glucose oxidase and the lipase meet the feed-grade additive standard, the palm fat powder meets the feed-grade standard, and the silicon dioxide and the calcium stearate meet the food-grade additive standard.
Preparation method of rumen-bypass glucose oxidase
A preparation method of rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting palm fat powder, and keeping the temperature to 55 ℃ for later use;
step two, taking 40% of glucose oxidase, 2% of lipase and 8% of silicon dioxide, uniformly mixing with the 34% of palm fat powder prepared in the step one, sieving with a 80-mesh sieve, transferring into a centrifugal granulator, stirring, taking out after a certain time, and sieving with a 60-mesh sieve;
and thirdly, taking the core particles with the particle size less than or equal to 60 meshes in the second step, putting the core particles into a centrifugal granulator again, and slowly adding 6% of calcium stearate and 10% of palm fat powder prepared in the first step to finally prepare the rumen bypass glucose oxidase.
As can be seen from Table 1, the method uses glucose oxidase with enzyme activity of 12000U/g as proenzyme, the ratio of the glucose oxidase in the prepared rumen-bypass glucose oxidase is 40%, the enzyme activity should be kept at 4800U/g, the enzyme activity is actually measured to be 4251.5U/g, the enzyme activity retention rate is 88.6%, only 11.4% is lost in the coating process, and the enzyme activity retention rate is higher.
TABLE 1 rumen bypass glucose oxidase Retention assay
Third, test of using effect of rumen-bypass glucose oxidase additive
Test method 1
1.1 design of the experiment
Three treatments are set in the test, namely a control group, a test 1 group and a test 2 group, the control group and the control group are respectively fed with basic ration (the ratio of the refined to the coarse of the ration is 50:50), and the test 1 group and the test 2 group are respectively and uniformly mixed with the basic ration for feeding according to the amount of 100mg/(kg x d) and 200mg/(kg x d). The rumen bypass glucose oxidase additive consists of the following components in percentage by weight: 40% of raw material enzyme, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase, and the palm fat powder is prepared according to the preparation method disclosed by the invention.
The pre-feeding period of the feeding test is 10 days, and the positive test period is 60 days.
1.2 test animals
60 Holstein cows with similar weight, fetal frequency and lactation amount are selected and randomly divided into 3 groups, each group has 20 repetitions, and each repetition has 1.
1.3 Experimental diets and feeding management
The experimental diet was prepared according to the nutritional requirements and feeding standards of cows, and consisted of mixed concentrate and corn silage, and the composition and nutritional ingredients are shown in table 2. The ratio of the refined grain to the coarse grain of the feed is 50:50, the test dairy cows are fed in a single groove, fed at 07:00 and 19:00 every day, and fed with free water and water.
Table 2 test basal diet composition and nutritional levels
Each kilogram of premix contains: 100mg cobalt, 8500mg copper, 50,000mg iron, 30,000mg manganese, 300mg zinc, 300mg iodine, 300mg selenium, 7500,000IU vitamin A, 1200,000IU vitamin D and 40,000IU vitamin E.
1.4 sample Collection and measurement
1.4.1 feed intake determination and feed sample Collection and analysis
During the test, the feed intake and the residual forage intake are recorded in detail day by day, the concentrated forage and the corn silage sample are collected according to the proportion every day, and the concentrated forage and the corn silage sample are stored for later use after the initial water is measured.
1.4.2 determination of milk Performance indicators
During the test period, the milk yield of each cow is recorded every day, 50mL of milk samples are collected according to the proportion of 4:3:3 in the morning, the noon and the evening, 0.6mg/mL of potassium dichromate is added, and the mixture is uniformly mixed and refrigerated at 4 ℃ for standby test. The collected milk samples are sent to a detection center, and a multifunctional milk component analyzer is used for detecting the milk protein rate, the lactose rate, the milk dry matter rate and the somatic cell number.
2 results of the test
2.1 Effect of rumen-bypass glucose oxidase on the Productivity of Holstein cows
As can be seen from table 3, the average daily milk yield of the test 1 and the test 2 groups was significantly higher than that of the control group, and the average daily food intake had no significant effect but tended to increase. The average daily milk yield of test 1 and test 2 groups increased by 12.1% and 20.7%, respectively.
TABLE 3 influence of rumen-bypass glucose oxidase on the Productivity of Holstein cows
Note: the same letters in the same row abc indicate no significant difference (P > 0.05), and the letters different indicate significant difference (P < 0.05).
2.2 Effect of rumen-bypass glucose oxidase on milk production Performance of Holstein cows
As can be seen from table 4, there was no significant difference in milk protein ratio and non-fat solids for each experimental group. The lactose rate and total milk solids of test 1 and test 2 groups were significantly higher than the control group, the milk fat rate was significantly higher than the control group, and the somatic cell count was significantly lower than the control group.
TABLE 4 influence of rumen-bypass glucose oxidase on milk production Performance of Holstein cows
Note: the same letters in the same row abc indicate no significant difference (P > 0.05), and the letters different indicate significant difference (P < 0.05).
3 conclusion of the test
The rumen bypass glucose oxidase additive can improve the milk yield of Holstein cows, improve the milk quality and improve the milk production performance.
Example 1
The rumen bypass glucose oxidase comprises 400g of glucose oxidase meeting the feed grade additive standard, 20g of lipase, 440g of palm fat powder meeting the feed grade standard, 80g of silicon dioxide meeting the food grade additive standard and 60g of calcium stearate.
The preparation method of the rumen bypass glucose oxidase comprises the following steps:
firstly, heating and melting 440g of palm fat powder, and keeping the temperature to 55 ℃ for later use;
step two, taking 400g of glucose oxidase, 20g of lipase and 80g of silicon dioxide, uniformly mixing with 340g of the palm fat powder obtained in the step one and heated and melted, sieving with a 80-mesh sieve, transferring into a centrifugal granulator, stirring, taking out after a certain time, and sieving with a 60-mesh sieve;
and thirdly, placing the core particles with the particle size not larger than 60 meshes in the second step into a centrifugal granulator again, and slowly adding 60g of calcium stearate and 100g of the heated and melted palm fat powder obtained in the first step to finally prepare the rumen bypass glucose oxidase.
Example 2
The rumen bypass glucose oxidase comprises 420g of glucose oxidase, 15g of lipase, 450g of palm fat powder, 90g of silicon dioxide and 70g of calcium stearate.
The raw material criteria and preparation method in this example are the same as in example 1.
Example 3
The rumen bypass glucose oxidase comprises 450g of glucose oxidase, 20g of lipase, 480g of palm fat powder, 90g of silicon dioxide and 70g of calcium stearate.
The raw material criteria and preparation method in this example are the same as in example 1.
Example 4
The rumen bypass glucose oxidase comprises 350g of glucose oxidase, 10g of lipase, 400g of palm fat powder, 70g of silicon dioxide and 60g of calcium stearate.
The raw material criteria and preparation method in this example are the same as in example 1.
The four embodiments of the present invention have been described in detail, but the description is only for the preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.
Claims (10)
1. A rumen bypass glucose oxidase, which is characterized in that: the weight percentage of each component is as follows: 35-45% of glucose oxidase, 40-50% of palm fat powder, 7-9% of silicon dioxide, 5-7% of calcium stearate and 1-3% of lipase.
2. The rumen bypass glucose oxidase according to claim 1, wherein: the weight percentage of each component is as follows: 40% of glucose oxidase, 44% of palm fat powder, 8% of silicon dioxide, 6% of calcium stearate and 2% of lipase.
3. A method of using rumen bypass glucose oxidase as claimed in any of claims 1 or 2, wherein: the rumen-bypass glucose oxidase is uniformly mixed with the concentrate supplement according to the proportion of 100-200mg per kilogram of body weight per day for feeding.
4. A preparation method of rumen-bypass glucose oxidase is characterized by comprising the following steps: the method comprises the following steps:
firstly, heating and melting palm fat powder, and keeping the temperature to 55 ℃ for later use;
step two, taking glucose oxidase, lipase and silicon dioxide, uniformly mixing the glucose oxidase, the lipase and the silicon dioxide with the palm fat powder prepared in the step one, sieving the mixture by a sieve of 80 meshes, transferring the mixture into a centrifugal granulator, stirring the mixture, taking out the mixture after a certain time, and sieving the mixture by a sieve of 60 meshes;
and thirdly, taking the core particles with the particle size less than or equal to 60 meshes in the second step, putting the core particles into a centrifugal granulator again, and slowly adding calcium stearate and the palm fat powder prepared in the first step to prepare the rumen-bypass glucose oxidase.
5. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the glucose oxidase added in the second step is 35-45%, and preferably, the weight percentage of the glucose oxidase added in the second step is 40%.
6. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the lipase added in the second step is 1-3%, and preferably, the weight percentage of the lipase added in the second step is 2%.
7. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the silica added in the second step is 7% -9%, preferably, the weight percentage of the silica is 8%.
8. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the palm fat powder added in the second step was 34%.
9. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the calcium stearate added in the third step is 5-7%, and preferably, the weight percentage of the calcium stearate is 6%.
10. The method for preparing rumen bypass glucose oxidase according to claim 4, wherein: the weight percentage of the palm fat powder added in the third step is 10%.
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| EP4356750A3 (en) * | 2021-07-30 | 2024-06-19 | Glasport Bio Limited | Compositions and methods |
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| US20050276900A1 (en) * | 2004-06-14 | 2005-12-15 | Ullanoormadam Sahasranamam R | Trans free non-hydrogenated hard structural fat and non-hydrogenated hard palm oil fraction component |
| CN103621979A (en) * | 2013-12-25 | 2014-03-12 | 张列飞 | Preparation and application of special compound premix for fermented feed |
| US20180338990A1 (en) * | 2017-05-24 | 2018-11-29 | Rupca Llc | Reduced Pressure Maillard Synthesis of Carbohydrate Energy Supplement for Ruminant Livestock |
| CN109619288A (en) * | 2018-12-11 | 2019-04-16 | 北京东方天合生物技术有限责任公司 | A kind of novel rumen glucose and the preparation method and application thereof |
| CN110637930A (en) * | 2019-06-11 | 2020-01-03 | 武瑞 | Rumen-protected amylase and preparation method thereof |
| CN111543550A (en) * | 2020-05-12 | 2020-08-18 | 肖鹏宏 | Whole-daily-grain compound feed for dairy cow feeding without grass and preparation method thereof |
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| US20050276900A1 (en) * | 2004-06-14 | 2005-12-15 | Ullanoormadam Sahasranamam R | Trans free non-hydrogenated hard structural fat and non-hydrogenated hard palm oil fraction component |
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| US20180338990A1 (en) * | 2017-05-24 | 2018-11-29 | Rupca Llc | Reduced Pressure Maillard Synthesis of Carbohydrate Energy Supplement for Ruminant Livestock |
| CN109619288A (en) * | 2018-12-11 | 2019-04-16 | 北京东方天合生物技术有限责任公司 | A kind of novel rumen glucose and the preparation method and application thereof |
| CN110637930A (en) * | 2019-06-11 | 2020-01-03 | 武瑞 | Rumen-protected amylase and preparation method thereof |
| CN111543550A (en) * | 2020-05-12 | 2020-08-18 | 肖鹏宏 | Whole-daily-grain compound feed for dairy cow feeding without grass and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4356750A3 (en) * | 2021-07-30 | 2024-06-19 | Glasport Bio Limited | Compositions and methods |
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