CN112625906B - Special culture dish of cardiac muscle cell and nerve cell - Google Patents
Special culture dish of cardiac muscle cell and nerve cell Download PDFInfo
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- CN112625906B CN112625906B CN202011601280.3A CN202011601280A CN112625906B CN 112625906 B CN112625906 B CN 112625906B CN 202011601280 A CN202011601280 A CN 202011601280A CN 112625906 B CN112625906 B CN 112625906B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to the technical field of culture dishes, and discloses a special culture dish for cardiac muscle cells and nerve cells, which can automatically and quantitatively add culture solution during cell culture by matching a fixed plate, a brake plate, a first disc and a liquid inlet pipe, avoids the need of opening a cover for adding liquid during cell culture, greatly reduces the pollution of external impurities to the cell culture, and is convenient to observe different positions by matching a second special-shaped disc, a third disc, a rotating rod and a control button.
Description
Technical Field
The invention relates to the technical field of culture dishes, in particular to a special culture dish for cardiac muscle cells and nerve cells.
Background
The culture dish is a laboratory vessel for culturing microorganisms or cells, and can be used for conveniently explaining the evolution of organisms, and the like, for example, the culture of myocardial cells and nerve cells has very important significance in the research of heart diseases and the treatment of nervous tissues at present. On the one hand, when carrying out cell culture, because high temperature heating leads to the loss of culture solution easily, so in order not to cause the influence to the cultivation of cell, need carry out the interpolation of culture solution many times, at present when adding the culture solution, need open the culture dish lid, then pour into the culture solution, but at the in-process of opening, make a large amount of impurity get into the culture tank in the outside air a little carelessly easily, cause the influence to the cultivation of cell, it influences to follow-up accuracy to experimental, on the other hand, carrying out the cell culture in-process, need use the microscope to observe, in order to make the observation abundant, need rotatory culture dish to observe cell each part, it is comparatively hard, and both hands direct contact culture dish when manual rotation, the bacterium on finger surface causes the pollution to the culture dish equally easily.
Aiming at the defects of the prior art, the special culture dish for the myocardial cells and the nerve cells has the advantages of automatically adding culture solution and automatically rotating during observation, solves the problem that the conventional culture dish is simple in arrangement, ensures that the nutrient solution can be automatically added in the cell culture process by using the device, does not need to open a culture dish cover, reduces bacterial pollution, improves the accuracy of an experimental result, and automatically rotates during culture dish observation, so that different positions can be conveniently observed, and time and labor are saved.
Disclosure of Invention
Technical scheme (I)
In order to realize the purposes of automatically adding culture solution and automatically rotating during observation, the invention provides the following technical scheme: the utility model provides a special culture dish of cardiac muscle cell and nerve cell, which comprises an outer shell, utilize the shell to support and stabilize whole device, the inside swing joint of shell has the culture dish body, utilize the culture dish body to carry out the cultivation of cell, the back swing joint of culture dish body has the dysmorphism dish, the rotation that utilizes the dysmorphism dish drives the culture dish body and removes in step, the surperficial swing joint of dysmorphism dish has the fixed plate, utilize the fixed plate to support the braking plate, the surperficial swing joint of fixed plate has braking plate and first disc, utilize opening and closing of braking plate control feed liquor pipe, thereby reach the purpose of automatic feed liquor, the braking plate is provided with two simultaneously, the specification of two braking plates is unanimous, and be the symmetric distribution for referring to the central line of fixed plate, the outer fixed surface of braking plate is connected with the feed liquor pipe, utilize the feed liquor pipe to carry out the transfer of culture solution, the below swing joint of first disc has the stop collar, the inside of stop collar is provided with the pivot, the top of this pivot is provided with conical gear, and conical gear's surface is provided with the runner, the rotation of utilizing the rotation of stop collar to drive runner synchronous rotation of runner, the right side swing joint of first gear has first gear, the left side of second swing joint, the second swing joint is connected with the third movable disc, the left side of second movable gear, the second movable disc is connected with the third movable gear, the third movable disc, the third movable gear is connected with the inside of movable knob, the third movable disc.
Preferably, the second disc, the second gear and the third disc are movably connected through a belt, and the belt is in a loose state in an original state.
Preferably, the second gear is provided as a missing gear, and the size of the second gear is matched with the size of the first gear.
Preferably, the surface of the first gear is provided with two fixing rods, and the two first gears are in the same specification and are movably connected through a belt.
Preferably, the surface of the limiting sleeve is provided with a chute, and the size of the chute is matched with that of the fixing rod arranged on the surface of the first gear.
Preferably, the inside of dysmorphism dish is seted up the spout, and this spout is provided with four, and the specification of four spouts is unanimous, and uses the central line of dysmorphism dish as the even distribution of reference.
Preferably, a pinion is arranged on the surface of the rotating rod, a fixed block is arranged on the surface of the pinion, teeth are arranged inside the shell, the size of the teeth is matched with that of the pinion, and the size of the fixed block is matched with that of a sliding groove formed inside the special-shaped disc.
(II) advantageous effects
Compared with the prior art, the invention provides a special culture dish for cardiac muscle cells and nerve cells, which has the following beneficial effects:
1. this special culture dish of cardiac muscle cell and nerve cell uses through the cooperation of fixed plate, braking plate, first disc and feed liquor pipe for when carrying out cell culture, can automatic ration add the culture solution, avoid carrying out cell culture when need opening the lid and carry out the liquid feeding, the pollution that external impurity caused to cell culture that has significantly reduced improves the accuracy of data.
2. This special culture dish of cardiac muscle cell and nerve cell uses through the cooperation of second sketch dish, three discs, bull stick and accuse button for when carrying out the culture dish observation, rotate automatically, thereby the convenience is observed the position of difference, does not need the manual position adjustment that carries on, labour saving and time saving more also avoids the pollution that the rotatory culture dish of both hands caused the culture dish simultaneously.
3. This special culture dish of cardiac muscle cell and nerve cell uses through the cooperation of stop collar, first gear, second gear, accuse button and third disc for link two steps of the rotation of the culture dish body and automatic addition culture solution, thereby increase the linkage nature of whole device, make the operation simpler.
Drawings
FIG. 1 is a schematic view of the structure of the present invention;
FIG. 2 is a cross-sectional view of FIG. 1 in accordance with the present invention;
FIG. 3 is an enlarged view of the structure of FIG. 2A according to the present invention.
In the figure: 1. a housing; 2. a culture dish body; 3. a shaped disc; 4. a fixing plate; 5. a brake plate; 6. a first disc; 7. a liquid inlet pipe; 8. a position limiting sleeve; 9. a first gear; 10. a second gear; 11. a second disc; 12. a third disc; 13. a rotating rod; 14. and a control button.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Please refer to fig. 1-3, a special culture dish for cardiac muscle cells and nerve cells, which comprises a housing 1, utilize housing 1 to support and stabilize the whole device, the inside swing joint of housing 1 has culture dish body 2, utilize culture dish body 2 to carry out the cultivation of cell, the back swing joint of culture dish body 2 has sketch disc 3, the rotation that utilizes sketch disc 3 drives culture dish body 2 and removes in step, the spout has been seted up to the inside of sketch disc 3, this spout is provided with four, the specification of four spouts is unanimous, and use the central line of sketch disc 3 as referring to evenly distributed, the rotation that utilizes sketch disc 3 drives culture dish body 2 and rotates synchronously, thereby can carry out intermittent type nature rotation observation automatically when making culture dish body 2 observe, do not need artifical the rotation, make during the observation more convenient, pollute the dish body when avoiding manual rotation simultaneously.
The surface swing joint of dysmorphism dish 3 has fixed plate 4, utilize fixed plate 4 to support brake plate 5, the surface swing joint of fixed plate 4 has brake plate 5 and first disc 6, and first disc 6 is provided with two, the specification of two first discs 6 is unanimous, and be symmetric distribution for referring to with the central line of fixed plate 4, the surface of first disc 6 is provided with the belt simultaneously, the both ends and two brake plate 5 swing joint of belt, thereby utilize first disc 6 to drive two brake plate 5 synchronous motion when rotating, utilize opening and closing of brake plate 5 control feed liquor pipe 7, thereby reach the purpose of automatic feed liquor, brake plate 5 is provided with two simultaneously, the specification of two brake plates 5 is unanimous, and be symmetric distribution for referring to with the central line of fixed plate 4, the outer fixed surface fixedly connected with feed liquor pipe 7 of brake plate 5, utilize pipe 7 to carry out the transfer of culture solution, thereby make can add the culture solution automatically when carrying out cell culture, need not open the lid of culture dish body 2, the feed liquor pollution that external impurity caused to cell culture has been reduced greatly.
Below swing joint of first disc 6 has stop collar 8, the inside of stop collar 8 is provided with the pivot, the top of this pivot is provided with conical gear, and conical gear's surface is provided with the runner, utilize the rotation of stop collar 8 to drive runner synchronous revolution, through belt swing joint between runner and the first disc 6, the chute has been seted up on the surface of stop collar 8, the size of this chute and the mutual adaptation of dead lever size that first gear 9 surface set up, two dead levers contact with the chute in proper order, thereby link 8 intermittent type nature clockwise and anticlockwise rotations of stop collar, the right side swing joint of stop collar 8 has first gear 9, utilize the rotation of first gear 9 to drive stop collar 8 synchronous revolution, the surface of first gear 9 is provided with the dead lever, and first gear 9 is provided with two, two specifications of first gear 9 are unanimous, and through belt swing joint, two first gear 9 synchronous revolutions, thereby drive two dead levers synchronous revolution, the left side swing joint of first gear 9 has second gear 10, second gear 10 lacks the gear 10 and sets up to the gear, and the size of first gear 10 and the size of first gear 9 is unanimous with each other, when second gear 10 opens the mutual adaptation, thereby it can stop for the interval to drive the intermittent type of second gear, thereby the interval to carry out the linkage, thereby enough time of every time of the intermittent type of the interval, the interval of the back of the rotation of the enough rotation that can carry out.
The left side swing joint of second gear 10 has second disc 11, pass through belt swing joint between second gear 10 and the third disc 12 three, the belt is in lax state during original state, when second disc 11 moves down, make the belt tighten, drive third disc 12 and rotate, final linkage culture dish body 2 synchronous revolution, conveniently observe, the top swing joint of second disc 11 has third disc 12, the back swing joint of third disc 12 has bull stick 13, the surface of bull stick 13 is provided with the pinion, the surface of this pinion is provided with the fixed block, tooth has been seted up to the inside of shell 1 simultaneously, the size of this tooth and the size of pinion are mutual adaptation, the size of fixed block and the inside spout size of seting up of special-shaped dish 3 are mutual adaptation simultaneously, the rotation that utilizes bull stick 13 drives the dead lever synchronous revolution of pinion surface, when the dead lever rotatory to contacting with the spout, the intermittent type of linkage special-shaped dish 3 is rotatory, the inside swing joint of shell 1 has accuse button 14, utilize 14 to control the rotation of culture dish body 2.
The working process and principle of the device are as follows: through the output shaft with the motor with second gear 10 swing joint, so can drive second gear 10 and rotate, the right side of second gear 10 is provided with first gear 9, and second gear 10 sets up to lacking the gear, can drive first gear 9 synchronous intermittent type nature when second gear 10 rotates and rotate, and can stop sufficient time when rotatory, first gear 9 is provided with two, through belt swing joint between two first gears 9, so drive two first gears 9 synchronous rotations, the surface of two first gears 9 all is provided with the dead lever, so can drive the dead lever when first gear 9 rotates and rotate in step, two dead levers contact with the chute that stop collar 8 surface was seted up in proper order, so drive stop collar 8 intermittent type nature and anticlockwise rotate, and can stop sufficient time after every rotation, the upper end of stop collar 8 is provided with the conical gear, the surface of this conical gear is provided with the runner, so drive the synchronous rotation of runner, pass through belt swing joint between runner and the first disc 6, simultaneously the outer end of first disc 6 is provided with, the both ends and two swing joint of brake belt 5, so open two swing joint brake plates 5, the automatic culture dish body that the pollution that the intermittent type culture dish body that the intermittent type does not need not open and the external culture medium body, thereby the automatic culture dish body that the pollution that the intermittent type culture liquid that has not caused.
Meanwhile, when the culture dish body 2 needs to be observed by a microscope, the control button 14 is pulled, so that the second disk 11, a belt between the third disk 12 and the second gear 10 is tightened, thereby the third disk 12 is driven to rotate, thereby the rotating rod 13 at the back of the third disk 12 is driven to rotate synchronously, the surface of the rotating rod 13 is provided with a pinion, meanwhile, the surface of the pinion is provided with a fixed block, so that the fixed block is driven to rotate synchronously, when the fixed rod rotates to the chute formed inside the special-shaped disk 3, the fixed rod is clamped between the fixed rod and the special-shaped disk 3, so that the special-shaped disk 3 is driven to rotate synchronously, the fixed rod rotates to rotate synchronously, the special-shaped disk 3 is driven to rotate synchronously, the fixed block is kept away from the chute, the clamping is lost, the special-shaped disk 3 stops rotating, so that the special-shaped disk 3 is finally driven to rotate synchronously and intermittently, the special-shaped disk 3 is arranged at the back of the culture dish body 2, so that the culture dish body 2 can rotate automatically when being observed, the position adjustment is not needed, thereby, different positions can be conveniently observed by two hands, and pollution of the culture dish caused by the contact of the culture dish can be avoided.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. A special culture dish of cardiac muscle cell and nerve cell, includes shell (1), its characterized in that: the culture dish is characterized in that a culture dish body (2) is movably connected inside the shell (1), a special-shaped disk (3) is fixedly connected to the surface of the culture dish body (2), a fixing plate (4) is movably connected to the surface of the special-shaped disk (3), a brake plate (5) and a first disk (6) are movably connected to the surface of the fixing plate (4), a liquid inlet pipe (7) is fixedly connected to the outer surface of the brake plate (5), a limiting sleeve (8) is movably connected to the lower portion of the first disk (6), a first gear (9) is movably connected to the right side of the limiting sleeve (8), a second gear (10) is movably connected to the left side of the first gear (9), a second disk (11) is movably connected to the left side of the second gear (10), a third disk (12) is movably connected to the upper portion of the second disk (11), a rotating rod (13) is movably connected to the back of the third disk (12), and a control button (14) is movably connected inside the shell (1);
the second disc (11), the second gear (10) and the third disc (12) are movably connected through a belt, and the belt is in a loose state in an original state;
a small gear is arranged on the surface of the rotating rod (13), a fixed block is arranged on the surface of the small gear, teeth are arranged inside the shell 1, the size of the teeth is matched with that of the small gear, and the size of the fixed block is matched with that of a sliding groove arranged inside the special-shaped disc (3);
when the second disc (11) moves downwards, the belt is tightened to drive the third disc (12) to rotate, and finally the culture dish body (2) is linked to rotate synchronously;
the second gear (10) is set to be a missing gear, and the size of the second gear (10) is matched with that of the first gear (9);
the surface of the first gear (9) is provided with two fixed rods, and the two first gears (9) are in the same specification and are movably connected through a belt;
the surface of the limiting sleeve (8) is provided with a chute, and the size of the chute is matched with that of a fixed rod arranged on the surface of the first gear (9);
the inside of dysmorphism dish (3) has been seted up the spout, and this spout is provided with four, and the specification of four spouts is unanimous, and uses the central line of dysmorphism dish (3) as reference evenly distributed.
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| CN202011601280.3A CN112625906B (en) | 2020-12-30 | 2020-12-30 | Special culture dish of cardiac muscle cell and nerve cell |
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| CN202011601280.3A CN112625906B (en) | 2020-12-30 | 2020-12-30 | Special culture dish of cardiac muscle cell and nerve cell |
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| CN112625906B true CN112625906B (en) | 2022-11-18 |
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| CN201366086Y (en) * | 2008-10-21 | 2009-12-23 | 佛山冯了性药业有限公司 | Packaging machine capable of automatically producing shells for wax pills |
| WO2012144983A1 (en) * | 2011-04-18 | 2012-10-26 | Empire Technology Development Llc | Revolving cell culture cartridge and methods of use |
| KR101554777B1 (en) * | 2013-07-29 | 2015-09-22 | 국립대학법인 울산과학기술대학교 산학협력단 | Cell culture device |
| WO2016208777A1 (en) * | 2015-06-26 | 2016-12-29 | 国立研究開発法人国立循環器病研究センター | Cell culture container |
| CN207862331U (en) * | 2017-11-03 | 2018-09-14 | 吉林农业大学 | A kind of culture solution quantifies continuous injecting |
| CN109593653B (en) * | 2018-12-13 | 2021-10-01 | 长春中医药大学 | Biological cell culture device |
| CN112080424B (en) * | 2020-09-05 | 2022-05-17 | 湖南师范大学 | Monitoring device for cell culture |
| CN112111401B (en) * | 2020-09-27 | 2023-05-26 | 广州正源生物技术有限公司 | Stem cell culture fluid preparation ware |
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Effective date of registration: 20221101 Address after: 518000 Financial Office of Land Watson Life Technology Center, Baguang Community, Kuichong Street, Dapeng New District, Shenzhen, Guangdong Applicant after: Shenzhen Letu Watson Precision Medical Co.,Ltd. Address before: 054000 Shahe standard parts Industrial Park, No.1, Nanhuan Road, Shahe City, Xingtai City, Hebei Province Applicant before: Cheng Shuge |
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