CN112616850A - Bactericidal composition containing prothioconazole and allyl isothiocyanate - Google Patents

Abstract

The invention relates to a bactericidal composition containing prothioconazole and allyl isothiocyanate, which comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from prothioconazole, and the active ingredient B is selected from allyl isothiocyanate. The weight ratio of the active ingredient A to the active ingredient B is 1: 80-80: 1, the composition can be prepared into a preparation formulation allowed by pesticides, the preferable formulation is wettable powder, water dispersible granules, a suspending agent and a microemulsion, and the composition can be used for preventing and treating diseases such as take-all, powdery mildew, gibberellic disease, gray mold, banded sclerotial blight, leaf spot, rust disease, sclerotinia, brown rot, anthracnose, ring spot, early blight, scab and the like, and has the characteristics of small dosage, rain wash resistance and obvious synergy.

Description

Bactericidal composition containing prothioconazole and allyl isothiocyanate

Technical Field

The invention belongs to the technical field of pesticides, and relates to application of a bactericidal composition containing prothioconazole and allyl isothiocyanate to crop diseases.

Technical Field

The english name of prothioconazole: prothioconazole, molecular formula: c₁₄H₁₅C_l2N₃OS chemical name: (RS) -2- [2- (1-Chlorocyclopropyl) -3- (2-chlorophenyl) -2-hydroxypropyl]-2, 4-dihydro-1, 2, 4-triazole-3-thione。

Prothioconazole is a novel triazolone systemic fungicide, is used as a demethylation inhibitor (DMI) and has the function of inhibiting the demethylation of lanosterol, a precursor of sterol in fungi, on 14-or-24-methylene dihydrolanosterol. Through a large number of field efficacy tests, the results show that the prothioconazole has good safety to crops, good effects of preventing and treating diseases and obvious yield increase, and compared with triazole bactericides, the prothioconazole has broad-spectrum sterilization and survival and long lasting period.

Allyl isothiocyanate has bactericidal, mildew-proof or antiseptic effects, and has inhibitory effect on target enzyme, i.e. root-knot nematode and soil-borne diseases such as sclerotinia, blight, etc., has fumigating and contact killing effects, and can be used as soil disinfectant.

In the actual process of agricultural production, the most easily generated problem of preventing and treating diseases is the generation of drug resistance of pathogenic bacteria, and the blending of different varieties of components is a common method for preventing and treating the generation of the resistance of the pathogenic bacteria. Active ingredients with different action mechanisms are mixed, and whether the synergistic, addition or antagonistic action is realized is judged according to the actual application effect. In most cases, the pesticide mixing effect is additive effect, the real synergistic mixing is less, and especially the obvious synergistic mixing is less. The research of the inventor finds that the mixing of prothioconazole and allyl isothiocyanate has good synergistic effect in a certain range, and relevant mixing of prothioconazole and allyl isothiocyanate is not reported at present.

Disclosure of Invention

The invention aims to provide a bactericidal composition containing prothioconazole and allyl isothiocyanate, which has a synergistic effect, low use cost and good control effect.

The technical scheme of the invention is as follows:

a bactericidal composition containing prothioconazole and allyl isothiocyanate comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is selected from prothioconazole, and the active ingredient B is selected from allyl isothiocyanate;

further, the weight ratio of the active ingredient A to the active ingredient B is 1: 50-50: 1;

further, the weight ratio of the active ingredient A to the active ingredient B is 1: 5-5: 1;

further, the weight ratio of the active ingredient A to the active ingredient B is 1: 2-2: 1;

further, the weight ratio of the active ingredient A to the active ingredient B is 1: 1.

The bactericide composition preparation can be prepared into a dosage form allowed by the pesticide field;

further, the composition comprises an active ingredient and an auxiliary ingredient of a pesticide preparation;

further, the auxiliary components of the pesticide preparation comprise a carrier and an auxiliary agent;

further, the carrier is any one, two or three of water, a solvent or a filler, and the water is preferably deionized water;

further, the solvent is selected from one or a mixture of more of N, N-dimethylformamide, cyclohexanone, toluene, xylene, dimethyl sulfoxide, methanol, ethanol, trimethylcyclohexanone, N-octylpyrrolidone, ethanolamine, triethanolamine, isopropylamine, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, ethanolamine, isopropylamine, ethyl acetate or acetonitrile;

further, the filler is selected from one or a mixture of more of kaolin, diatomite, bentonite, attapulgite, white carbon black, starch or light calcium carbonate;

furthermore, the auxiliary agent at least comprises a surfactant, and other functional auxiliary agents such as an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent, a defoaming agent and the like can be added according to different use occasions and requirements;

further, the surfactant is selected from one or more of an emulsifier, a dispersant, a wetting agent or a penetrant;

further, the other functional auxiliary agents are selected from one or more of an antifreezing agent, a thickening agent, a stabilizing agent, a disintegrating agent or a defoaming agent;

further, the emulsifier is selected from one or more of agricultural milk 500# (calcium alkyl benzene sulfonate), OP series phosphate ester (nonylphenol polyoxyethylene ether phosphate ester), 600# phosphate ester (phenylphenol polyoxyethylene ether phosphate ester), styrene polyoxyethylene ether ammonium sulfate salt, magnesium salt of alkyl diphenyl ether disulfonate, triethanolamine salt, agricultural milk 400# (benzyldimethylphenol polyoxyethylene ether), agricultural milk 700# (alkylphenol formaldehyde resin polyoxyethylene ether), Ningru 36# (phenethylphenol formaldehyde resin polyoxyethylene ether), agricultural milk 1600# (phenethylphenol polyoxyethylene polyoxypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), agricultural dehydrated milk 33# (alkylaryl polyoxyethylene polyoxypropylene ether), span series (sorbitan monostearate), Tween series (sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether) A mixture of seed constituents;

further, the dispersing agent is selected from one or a mixture of a plurality of polycarboxylate, lignosulfonate, alkylphenol polyoxyethylene formaldehyde condensate sulfate, calcium alkylbenzene sulfonate, naphthalene sulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene, fatty amine polyoxyethylene, fatty acid polyoxyethylene or glycerol fatty acid ester polyoxyethylene;

further, the wetting agent is selected from one or a mixture of more of sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, nekal BX, wetting penetrant F, Chinese honeylocust fruit powder, silkworm excrement or soapberry powder;

further, the penetrating agent is selected from a mixture consisting of one or more of penetrating agent JFC (fatty alcohol-polyoxyethylene ether), penetrating agent T (diisooctyl maleate sulfonate), azone or organic silicon;

further, the antifreezing agent is a mixture consisting of one or more of ethylene glycol, propylene glycol, glycerol or urea;

further, the thickening agent is selected from one or more of xanthan gum, polyvinyl alcohol, bentonite, carboxymethyl cellulose or magnesium aluminum silicate;

further, the stabilizer is selected from one or more of epoxidized soybean oil, epichlorohydrin, BHT, ethyl acetate and triphenyl phosphate;

further, the disintegrating agent is selected from one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate;

further, the defoaming agent is selected from one or a mixture of more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds;

all of the above are commercially available.

The sum of the contents of active ingredients, namely allyl isothiocyanate and prothioconazole, in the bactericidal composition is 1-90% of the total weight of the composition;

furthermore, the sum of the contents of active ingredients, namely allyl isothiocyanate and prothioconazole, in the bactericidal composition is 10-50% of the total weight of the bactericidal composition.

The bactericidal composition can be prepared into preparations acceptable in pesticides according to a method known by a person skilled in the art, and the preparations comprise solid preparations, liquid preparations, seed treatment preparations and other preparations;

further, the solid preparation is powder, dispersible tablets, granules, soluble powder, soluble granules, soluble tablets, milk powder, milk granules, water dispersible granules, wettable powder, micro-capsule granules, powder, macro-granules and water dispersible tablets;

further, the liquid preparation is microcapsule suspending agent, dispersible agent, missible oil, emulsion granules, oil emulsion, emulsion powder, aqueous emulsion, microemulsion, dispersible oil suspending agent, oil dispersion powder, suspending agent, suspoemulsion, soluble agent and ultra-low volume liquid;

further, the seed treatment preparation is a suspended seed coating agent, a seed treatment dry powder agent, a seed treatment dispersible powder agent, a seed treatment suspending agent and a seed treatment liquid agent;

further, the other preparation is bait, concentrated bait, gas preparation, aerosol, smoke agent;

furthermore, the preparation formulation is preferably wettable powder, water dispersible granules, suspending agents and microemulsions.

Further, the composition is prepared into wettable powder, and the components and the content of the wettable powder are preferably as follows: 0.1-80% of prothioconazole, 0.1-80% of allyl isothiocyanate, 2-10% of dispersant, 2-10% of wetting agent and the balance of filler;

further, the composition is prepared into water dispersible granules, and the components and the content of the water dispersible granules are preferably as follows: 0.1-80% of prothioconazole, 0.1-80% of allyl isothiocyanate, 3-12% of dispersant, 1-8% of wetting agent, 1-10% of disintegrating agent and the balance of filler.

Further, the composition is prepared into a suspending agent, and the components and the content of the suspending agent are preferably as follows: 0.1-80% of prothioconazole, 0.1-80% of allyl isothiocyanate, 2-10% of dispersant, 2-10% of wetting agent, 0.01-2% of defoaming agent, 0-2% of thickening agent, 0-8% of antifreeze agent and the balance of deionized water;

further, the composition is prepared into a microemulsion, and the components and the content of the microemulsion are preferably as follows: 0.1 to 80 percent of prothioconazole, 0.1 to 80 percent of allyl isothiocyanate, 5 to 15 percent of emulsifier, 2 to 10 percent of solvent, 5 to 10 percent of antifreeze agent, 0.5 to 3 percent of stabilizer and 100 percent of deionized water.

The invention has the following advantages: 1) the synergistic effect is obvious, the components are not simply superposed in activity, and the drug effect is greatly improved; 2) the prevention and treatment efficacy is outstanding, the duration is long, and the resistance risk is small; 3) the field dosage of the active ingredients is reduced, the production and use cost is reduced, and the pesticide residue and the environmental pollution are reduced; 4) the pesticide consists of active ingredients with different action mechanisms, and the action sites are increased, thereby being beneficial to overcoming and delaying the generation of the drug resistance of diseases.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more concise and clear, the present invention is described with the following specific embodiments, but the present invention is by no means limited to these embodiments. The following described examples are only preferred embodiments of the present invention, which can be used to describe the present invention and should not be construed as limiting the scope of the present invention. It should be understood that any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Preparation of the preparation

EXAMPLE 1 wettable powder of 18% prothioconazole-allyl isothiocyanate (5:1)

The formula is as follows: 15% of allyl isothiocyanate, 3% of prothioconazole, 3% of sodium naphthalene sulfonate formaldehyde condensate, 3% of wetting penetrant F and white carbon black;

the preparation method comprises the following steps: the active ingredients, the dispersing agent, the wetting agent and the filler are uniformly mixed, crushed by a jet mill and then uniformly mixed for the second time, and the wettable powder can be prepared.

Example 2: 15% wettable powder of prothioconazole-allyl isothiocyanate (2:1)

The formula is as follows: 10% of allyl isothiocyanate, 5% of prothioconazole, 6% of sodium lignin sulfonate, 4% of sodium dodecyl benzene sulfonate and kaolin are added for the rest;

the preparation method comprises the following steps: the same as in example 1.

Example 3: 30% prothioconazole-allyl isothiocyanate water dispersible granule (1:2)

The formula is as follows: 10% of allyl isothiocyanate, 20% of prothioconazole, 6% of fatty alcohol polyoxyethylene ether, 2% of sodium dodecyl benzene sulfonate, 2% of magnesium aluminum silicate, 0.5% of sodium bicarbonate and the balance of white carbon black;

the preparation method comprises the following steps: the active ingredients, the dispersing agent, the wetting agent, the binder, the disintegrating agent and the filler are subjected to airflow crushing to obtain the required particle size, and then the particles are sent into a fluidized bed granulation dryer to be granulated and dried, so that the water dispersible granule can be prepared.

Example 4: 12% prothioconazole-allyl isothiocyanate water dispersible granule (1:5)

The formula is as follows: 2% of allyl isothiocyanate, 10% of prothioconazole, 4% of fatty alcohol polyoxyethylene ether, 5% of sodium dodecyl sulfate, 2% of magnesium aluminum silicate, 2% of aluminum chloride and argil, and the balance is made up; the preparation method comprises the following steps: the same as in example 3.

Example 5: 15% prothioconazole allyl isothiocyanate suspending agent (2:1)

The formula is as follows: 10% of allyl isothiocyanate, 5% of prothioconazole, 3% of polyoxyethylene ether, 3% of sodium dodecyl sulfate, 2% of triethylene glycol, 2% of organic silicon, 3% of magnesium aluminum silicate and deionized water for supplementing the balance;

the preparation method comprises the following steps: the suspending agent is prepared by uniformly mixing the dispersing agent, the wetting agent, the defoaming agent, the thickening agent, the anti-freezing agent and other auxiliary agents through high-speed shearing, adding the active ingredients, complementing the balance with deionized water, and grinding in a ball mill for 2-3 hours until the particle size of particles is below 5 um.

Example 6: 40% prothioconazole allyl isothiocyanate suspending agent (1:1)

The formula is as follows: 20% of allyl isothiocyanate, 20% of prothioconazole, 3% of polyoxyethylene ether, 3% of sodium dodecyl sulfate, 2% of triethylene glycol, 4% of organic silicon, 3% of gelatin and deionized water;

the preparation method comprises the following steps: the same as in example 5.

Example 7 microemulsion of 12% Prothioconazole-allyl isothiocyanate (1:5)

The formula is as follows: 2% of allyl isothiocyanate, 10% of prothioconazole, 6% of fatty alcohol-polyoxyethylene ether, 4% of alkylbenzene sulfonate, 33# 5% of agricultural emulsion, 5% of polyethylene glycol, 2% of gelatin, 0.8% of silicone oil and the balance of deionized water;

the preparation method comprises the following steps: adding the original drug, the solvent and the emulsifier into a mother liquor preparation kettle to prepare a uniform oil phase, uniformly mixing deionized water, an antifreeze agent and the like, injecting into a product preparation kettle, stirring at a high speed, and uniformly mixing to prepare the transparent or semitransparent microemulsion product of the composition.

Example 8: 11% prothioconazole allyl isothiocyanate microemulsion (10:1)

The formula is as follows: 10% of allyl isothiocyanate, 1% of prothioconazole, 4% of alkylbenzene sulfonate, 5% of alkylphenol polyoxyethylene, 7% of OP series, 4% of propylene glycol, 2% of polyvinyl alcohol, 0.8% of silicone oil and the balance of deionized water;

the preparation method comprises the following steps: the same as in example 7.

Active case

Example 9: the indoor combined test of the wheat take-all disease by mixing prothioconazole and allyl isothiocyanate.

The test basis is as follows: NY/T1156.2-2006 agricultural chemical indoor bioassay test guidelines part 2 of fungicides: the 6 th part of bactericide of experimental criteria of indoor biological assay of pesticide, the plate method of tests for inhibiting the growth of pathogenic fungi hypha, NY/T1156.6-2006: combined action assay of compounding.

Test targets: wheat take-all disease.

The test instrument: electronic balances, nebulizers, microscopes, sterilizers, clean bench, biochemical incubators, hemocytometer, pipettors, inoculators, punches, calipers, and the like.

Preparing a test material: culturing wheat take-all pathogen on medium for use.

Test agents: 95% of prothioconazole and 94% of allyl isothiocyanate, which are provided by the research and development center of the Heliel pharmaceutical industry group.

Preparing a medicament: dissolving the raw materials with methanol, diluting with 0.1% Tween 80 water solution, respectively preparing single mother liquor, and setting 5 series of mass concentrations for use.

Medicament treatment: under the aseptic condition, sequentially and quantitatively sucking the liquid medicine from low concentration to high concentration, respectively and quantitatively adding the pre-melted sterile culture medium into a sterile conical flask, fully shaking up, and then pouring the liquid medicine into more than 3 culture dishes with the diameter of 9cm in an equivalent manner to prepare the medicine-containing flat plates with corresponding concentrations.

Inoculation: and (3) cutting the cultured pathogenic bacteria from the edges of bacterial colonies by using a sterilization puncher with the diameter of 5mm under the aseptic condition, inoculating the bacterial cake to the center of a drug-containing flat plate by using an inoculator, covering a dish cover, and placing the flat plate in an incubator at a proper temperature for culturing.

And (3) investigation: and (5) investigating the growth condition of the hyphae of the pathogenic bacteria according to the growth condition of the bacteria in the blank control culture dish. The colony diameter was measured in mm with a caliper. The diameter of each colony was measured perpendicularly by the cross method once and averaged.

Data statistics and analysis:

colony growth value diameter-colony diameter-fungus cake diameter

Hypha growth inhibition rate (blank control colony growth diameter-treatment zone colony growth diameter) × 100 ÷ blank control colony growth diameter.

Sun Yupei method: the synergy of the mixed medicament is evaluated according to the co-toxicity coefficient (CTC), namely the CTC is less than or equal to 80 and is antagonistic, the CTC is between 80 and 120 and is additive, and the CTC is more than or equal to 120 and is synergistic. The co-toxicity coefficient calculation formula is as follows:

the measured virulence index of the mixture is equal to the EC of the standard medicament₅₀EC of/mixture₅₀×100

The theoretical virulence index of the mixture is the virulence index of the medicament A, the percentage of the medicament A in the mixture, the virulence index of the medicament B and the percentage of the medicament B in the mixture

Co-toxicity coefficient (CTC) ═ actually measured virulence index of the mixture/theoretical virulence index of the mixture × 100

And (3) test results: see table 1.

TABLE 1 Combined virulence results for Prothioconazole and allyl isothiocyanate compounding on wheat take-all disease

The test results are known (see table 1): when the mixing ratio of prothioconazole and allyl isothiocyanate is 1: 50-50: 1 for preventing and treating wheat take-all, the co-toxicity coefficients are all larger than 80, which indicates that the mixing ratio of prothioconazole and allyl isothiocyanate is in the range of 1: 50-50: 1, and the additive or synergistic effect is shown; when the mixing ratio of prothioconazole and allyl isothiocyanate is 10: 1-1: 5, the co-toxicity coefficients are all larger than 120, and the synergistic effect is shown; when the mixing ratio of prothioconazole to allyl isothiocyanate is 1: 5-5: 1, the co-toxicity coefficients are all larger than 140, which shows obvious synergy; when the mixing ratio of prothioconazole to allyl isothiocyanate is 1: 2-2: 1, the co-toxicity coefficients are all larger than 170, which shows that the synergy is very obvious; when the mixing ratio of prothioconazole to allyl isothiocyanate is 1:1, the synergy is most obvious, and the co-toxicity coefficient is 207.74.

Pesticide effect in field

Example 10: prothioconazole and allyl isothiocyanate are mixed to prevent and treat wheat take-all

The test basis is as follows: GB/T17980.109-2004 part 109 of pesticide field efficacy test guidelines (II): bactericide for preventing and treating wheat take-all disease

Test targets: wheat take-all; the wheat variety 'Xiaoyan-22'.

Environmental conditions: the test is carried out in northern Xingfu village of Weifang Changyi city, Shandong province, the test land is flat land, loam and has about 1 percent of organic matter content and no weed on the ground.

Test agents: 40% prothioconazole allyl isothiocyanate suspension (1: 1).

Control agents: 40% of allyl isothiocyanate suspending agent, 20% of thiophanate-propyl suspending agent and clear water as reference.

Cell area and repetition: the cell area was 20 square meters, with 4 repetitions per treatment.

The application method comprises the following steps: spraying treatment is started 5 months and 11 days at the initial stage of wheat take-all, and a second time of medicine application is carried out after 7 days (5 months and 18 days) for 2 times.

Investigation and recording: the disease condition base was investigated before the drug (5 months and 15 days), the intermediate-stage results were investigated 7 days (5 months and 22 days) after the first drug, and the final results were investigated 14 days (6 months and 5 days) after the 2 nd drug.

Sampling is carried out at 5 points on the diagonal of each cell, 20 root samples are investigated at each point, and the percentage of infection of each root is investigated and graded. The classification method comprises the following steps:

level 0: no disease;

level 1: the disease area of the root system accounts for 1 to 5 percent;

and 3, level: the disease area of the root system accounts for 6 to 20 percent;

and 5, stage: the disease area of the root system accounts for 21 to 40 percent;

and 7, stage: the disease area of the root system accounts for 41 to 60 percent;

and 9, stage: the disease area of the root system accounts for more than 61 percent.

The pharmacodynamic calculation mode is as follows:

disease index ∑ (root coefficient at each level × relative level value)/(total root number × 9) × 100

Control effect (%) [1- (control area pre-drug disease index x treatment area post-drug disease index)/(control area post-drug disease index x treatment area pre-drug disease index) ] × 100

And (3) test results:

table 2 Prothioconazole and allyl isothiocyanate mixed control effect on wheat take-all disease

The test results show (see table 2): 7 days after the first application, the treatment of prothioconazole and allyl isothiocyanate respectively shows good control effect on wheat take-all, namely 68.75%, 75.00% and 82.65%; 14 days after the second medicine, the control of the wheat take-all disease by the treatment of the prothioconazole and the allyl isothiocyanate is higher than 70 percent, and the control is 72.73 percent, 75.26 percent and 84.35 percent respectively.

Example 11: prothioconazole and allyl isothiocyanate are mixed and mixed to prevent and treat rice sheath blight disease field drug effect

Test targets: rice sheath blight disease (Rhizoctonia solani), rice, variety 41.

Test site: the test was carried out in dragon Baicun paddy fields in inkstone county, Yunnan province.

Test agents: test agents: 40% prothioconazole allyl isothiocyanate suspension (1: 1).

Control agents: 40% of allyl isothiocyanate suspending agent, 20% of thiophanate-propyl suspending agent and clear water as reference.

The application method comprises the following steps: the medicine is applied at the early stage of the onset of rice sheath blight disease in 2016, 7, 4 and 2 times at intervals of 7 days. Each treatment was repeated 4 times, 20 square meters per cell, with cells randomly arranged. The spraying agent is uniformly sprayed on the front and back surfaces of the blade by a conventional spraying method.

The investigation and statistical method comprises the following steps: the disease condition was examined before application, and 7 days and 14 days after application. Five points are sampled on the diagonal line of each cell, 5 connected clusters are taken at each point, 25 clusters of rice are investigated in total, and the healthy plant number and the diseased plant number at each level are recorded.

The diseases are classified according to the following method:

level 0: disease free

Level 1: the fourth leaf and the sheath and leaf of the following leaves (the sword leaf is the first leaf)

And 3, level: the third leaf and the sheath and leaf of each leaf below the third leaf

And 5, stage: the second leaf and the leaf sheath and leaf of the following leaf

And 7, stage: attack of scabies and the sheaths and leaves of the sword-like leaves

And 9, stage: the whole plant is attacked and withered in advance.

Disease rate (%) (100 × number of diseased plants/total number of investigated plants);

disease index [ Σ (number of diseased plants at each stage × number of relative stage)/(total number of investigated plants × 9) ] × 100

The preventing and treating effect (%) is [ (disease index after blank group application-disease index after treatment group application)/disease index after blank group application ] × 100

And (3) test results:

table 3 Prothioconazole and allyl isothiocyanate are mixed and mixed to control rice sheath blight disease

The test results show that (see table 3), the effect of the blending treatment of prothioconazole and allyl isothiocyanate on preventing and treating rice sheath blight is obviously higher than that of a single agent, and the prevention effects of the treatment 14 days after the agent treatment are 76.63%, 80.68% and 84.45% respectively.

Example 12: prothioconazole and allyl isothiocyanate are mixed and mixed to prevent and treat brown rot of peach in field

Test targets and varieties: brown rot of peach, medium oil No. 4.

Test site: the great-tile house shop Yuan Tai Zhen Dawancun.

Reagent to be tested: 40% allyl isothiocyanate propiconazole suspension (1:1)

Control agents: 40% of allyl isothiocyanate suspending agent, 20% of thiophanate-propyl suspending agent and clear water as reference.

The application method comprises the following steps: the first application is carried out in the early stage of flower metabolism, and the second application is carried out at an interval of 14 days after the first application. The disease and fruit rate is investigated once before the pesticide is applied, the investigation is carried out once again 14 days after the last pesticide application, two plants in each district are investigated, each plant is sampled at 4 points of east, west, south and north, 100 leaves are taken for investigation according to the situation at each point, the disease leaf rate and the disease index are counted, and the prevention effect is calculated.

Control effect (%) (1- (control area pre-drug disease index)/(control area post-drug disease index)/(control area pre-drug disease index))) 100

And (3) test results:

TABLE 4 prevention and treatment effects of a mixture of prothioconazole and allyl isothiocyanate on brown rot of peach

The test results show that (see table 3), the control effect of the prothioconazole and allyl isothiocyanate mixed treatment area is over 60 percent, and is superior to that of a single agent.

During the treatment period of each test agent, the mixed components do not cause phytotoxicity to the development of peach trees and peach fruits.

Claims (10)

1. A bactericidal composition containing prothioconazole and allyl isothiocyanate is characterized in that: the composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is allyl isothiocyanate, and the active ingredient B is prothioconazole.

2. The bactericidal composition of claim 1, wherein the weight ratio of active ingredient a to active ingredient B is 1:50 to 50: 1.

3. The bactericidal composition of claim 1, wherein the weight ratio of active ingredient a to active ingredient B is 1:5 to 5: 1.

4. The bactericidal composition of claim 1, wherein the weight ratio of active ingredient a to active ingredient B is 1:2 to 2: 1.

5. The bactericidal composition of claim 1, wherein the weight ratio of active ingredient a to active ingredient B is 1: 1.

6. The bactericidal composition of claim 1, wherein the sum of the active ingredients in the composition is from 1% to 90%, preferably from 10% to 50%, of the total weight of the composition.

7. The bactericidal composition of claim 1, wherein the composition is formulated in any form suitable for agricultural use; preferably, the dosage form comprises any one of powder, dispersible tablet, granule, soluble powder, soluble granule, soluble tablet, emulsion powder, emulsion granule, water dispersion granule, wettable powder, microcapsule granule, powder, large granule, water dispersion tablet, microcapsule suspension, dispersible liquid, missible oil, emulsion granule, oil emulsion, emulsion in water, microemulsion, dispersible oil suspension, oil dispersion powder, suspension, suspoemulsion, soluble liquid, ultra-low volume liquid, suspension seed coating agent, dry seed treatment powder, dispersible seed treatment powder, suspension for seed treatment, and liquid for seed treatment; the preferable preparation formulation is wettable powder, water dispersible granules, suspending agent and microemulsion.

8. Use of the fungicidal composition according to any one of claims 1 to 7 for controlling crop diseases; preferably, the diseases comprise full rot, powdery mildew, gibberellic disease, gray mold, banded sclerotial blight, fusarium wilt, leaf spot, rust disease, sclerotinia rot, brown rot, anthracnose, ring spot, early blight and scab.

9. The use of the bactericidal composition according to claim 8 for controlling crop diseases, wherein the diseases are wheat take-all, rice sheath blight or peach brown rot.

10. A method for controlling crop diseases, which is characterized in that the bactericidal composition as defined in any one of claims 1 to 8 is sprayed on the surfaces of crops or is uniformly stirred with crop seeds.