CN112616773A - Production method for improving antioxidant component in embryo eggs - Google Patents
Production method for improving antioxidant component in embryo eggs Download PDFInfo
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- CN112616773A CN112616773A CN202011445950.7A CN202011445950A CN112616773A CN 112616773 A CN112616773 A CN 112616773A CN 202011445950 A CN202011445950 A CN 202011445950A CN 112616773 A CN112616773 A CN 112616773A
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- 235000013601 eggs Nutrition 0.000 title claims abstract description 132
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 103
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 65
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 63
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 21
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 49
- 239000001301 oxygen Substances 0.000 claims abstract description 49
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 49
- 230000003115 biocidal effect Effects 0.000 claims abstract description 33
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 20
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 20
- 210000003278 egg shell Anatomy 0.000 claims abstract description 20
- 239000003242 anti bacterial agent Substances 0.000 claims abstract description 15
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- 230000028327 secretion Effects 0.000 claims abstract description 12
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- 239000004365 Protease Substances 0.000 claims abstract description 9
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 9
- 108090000145 Bacillolysin Proteins 0.000 claims abstract description 8
- 229940088710 antibiotic agent Drugs 0.000 claims abstract description 8
- 102000035195 Peptidases Human genes 0.000 claims abstract description 7
- 230000003647 oxidation Effects 0.000 claims abstract description 7
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 7
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- 108091005658 Basic proteases Proteins 0.000 claims abstract description 6
- 230000001737 promoting effect Effects 0.000 claims abstract description 5
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- 238000000034 method Methods 0.000 claims description 9
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- 238000011049 filling Methods 0.000 claims description 6
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- 239000007924 injection Substances 0.000 claims description 6
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 2
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
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- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001122767 Theaceae Species 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
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- 230000004792 oxidative damage Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K41/00—Incubators for poultry
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a production method for improving antioxidant components in embryo eggs, which utilizes the inertia activity rule of life activities, firstly the radian formed by a plurality of rows of cannula is the same as the radian formed by the outer surface of the embryo eggs, thus, the punching density of the upper surface of the embryo egg is the same, the inner part of the embryo egg is uniformly contacted with the outside, the force applied to the egg shell of the embryo is uniformly distributed, the breakage of the egg shell of the embryo is prevented, simultaneously, the injected antibiotics are uniformly contacted with the inner part of the embryonic egg, so that the fertilized egg begins to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products under the influence of the external environment, and then the secretion of acid protease and neutral protease is inhibited through the created oxygen-rich and antibiotic inhibition environment, thereby promoting the growth of the alkaline protease, greatly improving the oxidation resistance components in the embryo eggs and having better creativity and practicability.
Description
Technical Field
The invention relates to the field of biology, in particular to the field of a production method for improving an antioxidant component in an embryo egg.
Background
As is well known, antioxidants are widely used in the fields of foods, health products, daily chemical products, and the like. On one hand, the antioxidant can prevent the above products from discoloring or deteriorating due to oxidation; on the other hand, some oxidants even play active functional roles in related products, such as playing roles of resisting oxidation and scavenging free radicals in vivo, in recent years, people actively search for natural and safe antioxidants, and find the existence of various antioxidant compounds, such as vitamin E, tea polyphenol and the like, in many natural animal and plant materials, but most of the existing raw materials for preparing the antioxidant peptide are substances with low reserves in the environment, and at this time, an object with high reserves in the environment is required to be used for preparing the antioxidant compound so as to reduce the production cost of the antioxidant peptide.
The biological phenomenon that the fertilized eggs can perform embryo development at a specific temperature, and the embryos are in a high-concentration oxygen environment without oxidative damage shows that some antioxidant substances in the embryo eggs can protect the normal development of the embryos, and during the hatching period, the proteins in the embryo eggs are converted into nutrient substances which can be absorbed by the embryos and must be firstly degraded into low-molecular-weight peptides or amino acids, which provide advantages for preparing antioxidant active peptides from the embryo eggs, but the content of the antioxidant ingredients in the embryo eggs is low, so that the extraction has higher production cost, and how to increase the content of the antioxidant active ingredients in the embryo eggs does not have a proper solution in the prior art.
Disclosure of Invention
The invention mainly aims to provide a production method for improving antioxidant ingredients in embryo eggs, which can effectively solve the problems in the background technology: the content of antioxidant ingredients in the embryo eggs is still less, the production cost is higher even if the antioxidant ingredients are extracted, and no suitable solution is provided in the prior art for how to improve the content of the antioxidant active ingredients in the embryo eggs.
In order to achieve the purpose, the invention adopts the technical scheme that: a method for producing an antioxidant in an embryo egg, the method comprising:
the method comprises the following steps: cleaning the fertilized eggs which are just born, vertically placing the fertilized eggs in the fixed grooves on the upper surface of the storage tray, placing one fertilized egg in each fixed groove, movably inserting the slots on the side surface of the storage tray into the sliding plates on the inner surface of the culture cabin, placing the storage tray in the culture cabin, and culturing the embryo eggs in the incubator for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration;
step two: after the step one is finished, the control screen I controls the cylinder to be pressed down integrally, the lantern ring slides on the outer surface of the guide rail in the sliding groove, the spray head drives the insertion tubes to chisel the upper surface of the fertilized egg, the multiple rows of insertion tubes are arranged in a certain radian, the radian formed by the multiple rows of insertion tubes is the same as the radian formed by the outer surface of the embryo egg, thus the hole-chiseling density on the upper surface of the embryo egg is the same, the inside of the embryo egg is in uniform contact with the outside, the force applied to the embryo egg shell is uniformly distributed, and the embryo egg shell is prevented from being broken;
step three: the fertilized eggs are influenced by the external environment to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products so as to resist the influence of the external environment;
step four: the first control screen controls the cylinder to be pressed down integrally again, the lantern ring slides on the outer surface of the guide rail in the sliding groove, and antibiotics in the antibiotic storage box are injected into the fertilized eggs through the antibiotic output pipe, the feed pipe and the injection port and the intubation tube to generate antioxidant ingredients;
step five: after the fourth step is finished, the second display screen controls the reaction in the reaction kettle to be rapidly carried out, a large amount of oxygen enters the culture cabin through the gas filter, the flow valve and the oxygen inlet, so that the oxygen concentration in the culture cabin reaches ninety percent, the embryo eggs generate a large amount of antioxidant components to resist oxidation, and after oxygen enrichment supply is carried out for one day, the oxygen concentration in the culture cabin reaches the normal level of the outside air;
step six: and after the fifth step is finished, continuously reducing the oxygen concentration in the culture cabin, and generating a large amount of antioxidant substances due to the biological inertia effect, wherein the content of the antioxidant ingredients in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin for extracting the antioxidant peptides.
The invention further improves the device and the method, and the device also comprises a production device for improving the antioxidant components in the embryo eggs, the device comprises an incubator and a reaction kettle, the reaction kettle is fixedly arranged on the upper surface of the incubator, an antibiotic storage box is fixedly arranged on the back side of the incubator, the antibiotics in the antibiotic storage box have the chemical characteristics of inhibiting the growth of acidic and neutral proteases, the incubator is internally provided with an incubator, the middle part of the incubator is provided with a storage disc, the incubator is internally provided with a hole drilling mechanism which is positioned right above the storage disc, and the output end of the reaction kettle is connected with a flow controller through a gas pipeline.
The invention is further improved in that the flow controller is suitable for controlling the flow of oxygen to control the reaction degree in the reaction kettle, the output end of the reaction kettle is connected with a gas filter through a pipeline, the gas filter is suitable for filtering gas generated by reaction in the reaction kettle to prevent other gas generated in the reaction kettle from generating side effects on the growth of embryo eggs, the output end of the gas filter is connected with a culture cabin through a pipeline, a first control screen is fixedly arranged on the right side of the culture cabin, the first control screen controls the environment in the culture cabin through a plc system, a feeding pipe is arranged on the right side of the culture cabin and below the first control screen, a chute is arranged on the inner surface of the culture cabin, a sliding plate is fixedly arranged on the side surface of the culture cabin, and a guide rail is fixedly arranged in the chute, cultivate the inboard of cabin and be located the top of slide and seted up the oxygen entry, the oxygen entry is evenly arranged at the internal surface of cultivateing the cabin, the last fixed surface who cultivates the cabin installs the cylinder, the output of cylinder and the last fixed surface who cuts a hole the mechanism are connected, the electrical property input and the control panel electric connection of a cylinder.
The invention has the further improvement that a second display screen is fixedly arranged on the outer surface of the reaction kettle, the second display screen is suitable for controlling and accurately controlling the reaction degree in the reaction kettle, an upper cover is covered on the upper surface of the reaction kettle, a first raw material pipe and a second raw material pipe are arranged on the upper surface of the upper cover, and the first raw material pipe and the second raw material pipe are suitable for adding raw materials for producing oxygen.
The invention is further improved in that an antibiotic output tube is fixedly installed at the output end of the antibiotic storage box, slots are formed in two sides of the storage tray, the inner surfaces of the slots are inserted into sliding plates, a fixing groove is formed in the upper surface of the storage tray, a protection pad is fixedly installed on the inner surface of the fixing groove, the diameter of the fixing groove is matched with the transverse diameter of the embryo egg, so that the embryo egg can be conveniently taken out and vertically fixed, the hole drilling mechanism comprises a connecting plate, a spray head is fixedly installed on the lower surface of the connecting plate, a plurality of rows of insertion tubes are fixedly installed on the lower surface of the spray head and are arranged in a certain radian, the radian formed by the plurality of rows of insertion tubes is the same as the radian formed by the outer surface of the embryo egg, so that the hole drilling density of the upper surface of the embryo egg is the same, the inside of the embryo egg is uniformly contacted with the outside, prevent that embryo's eggshell from breaking, the dorsal part fixed mounting of connecting plate has the lantern ring, the internal surface and the guide rail activity of the lantern ring cup joint, the upper surface and the cylinder fixed connection of connecting plate, the filling opening has been seted up on the right side of connecting plate, the output and the intubate of filling opening switch on, the input and the pan feeding pipe of filling opening switch on, and under the promotion of cylinder, the vertical lift of chisel hole mechanism can be stabilized like this and the operation is chisel hole and evenly injected to embryo's egg.
The invention further improves that the optimal hatching condition of the fertilized egg is that the fertilized egg is placed in a hatching box with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for hatching for 6-7d, thus facilitating the synthesis and secretion of antioxidant substances in the embryo egg.
The invention is further improved in that the antibiotic contains a large amount of components for inhibiting the secretion of the acid protease and the neutral protease to inhibit the secretion of the acid protease and the neutral protease, thereby promoting the growth of the alkaline protease.
Compared with the prior art, the invention has the following beneficial effects:
compared with the prior art, the invention utilizes the inertia activity rule of life activity, the radian formed by a plurality of rows of cannulas is the same as the radian formed by the outer surface of an embryo egg, thus the punching density of the upper surface of the embryo egg is the same, the inner part of the embryo egg is uniformly contacted with the outside, the force applied to the egg shell of the embryo is uniformly distributed, the egg shell of the embryo is prevented from being broken, and the injected antibiotic is uniformly contacted with the inner part of the embryo egg, so that the fertilized egg starts to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the external environment, and then the secretion of acid protease and neutral protease is inhibited by the created antibiotic inhibition environment, thereby promoting the growth of alkaline protease, and the created oxygen-enriched environment is beneficial to the generation of antioxidant active ingredient substances, thus the antioxidant ingredient in the embryo egg can be greatly improved, has better creativity and practicability.
Drawings
FIG. 1 is a schematic view of the overall structure of a production apparatus for increasing antioxidant content in an embryo egg according to the present invention.
FIG. 2 is a schematic view of the internal structure of the incubator of the apparatus for producing an egg with an increased antioxidant content according to the present invention.
FIG. 3 is a schematic view of a punching mechanism of a production apparatus for increasing antioxidant components in an embryo egg according to the present invention.
FIG. 4 is a schematic view of a nozzle of an apparatus for producing an egg with an increased antioxidant content according to the present invention.
FIG. 5 is a schematic flow chart of a method for producing an antioxidant supplement in an egg according to the present invention.
In the figure: 1. an incubator; 2. a reaction kettle; 3. an antibiotic storage tank; 4. a culture compartment; 5. storing a disc; 7. a hole drilling mechanism; 8. a flow controller; 9. a gas filter; 10. a flow valve; 101. a first control screen; 102. a feeding pipe; 103. a chute; 104. a slide plate; 105. a guide rail; 106. an oxygen inlet; 107. a cylinder; 201. a second display screen; 202. an upper cover; 203. a first feedstock pipe; 204. a second raw material pipe; 301. an antibiotic output tube; 501. a slot; 502. fixing grooves; 503. a pad; 701. a spray head; 702. inserting a tube; 703. a collar; 704. and (4) injecting the liquid into the opening.
Detailed Description
In order to make the technical means, the original characteristics, the achieved objects and the functions of the present invention easy to understand, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate the orientation or the positional relationship based on the orientation or the positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, but not for indicating or implying that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and thus not be construed as limiting the present invention. Furthermore, the terms "a," "an," "two," and "three" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. The invention will be further illustrated with reference to specific embodiments.
Example 1
As shown in figures 1-4, a production method for improving antioxidant components in embryo eggs comprises a production device for improving antioxidant components in embryo eggs, the device comprises an incubator (1) and a reaction kettle (2), the reaction kettle (2) is fixedly installed on the upper surface of the incubator (1), an antibiotic storage tank (3) is fixedly installed on the back side of the incubator (1), antibiotics in the antibiotic storage tank (3) have chemical characteristics of inhibiting the growth of acidic and neutral proteases, the incubator (4) is arranged in the incubator (1), a storage tray (5) is arranged in the middle of the incubator (1), a hole drilling mechanism (7) is arranged in the incubator (4) and right above the storage tray (5), and the output end of the reaction kettle (2) is connected with a flow controller (8) through an air conveying pipeline.
The flow controller (8) is suitable for controlling the flow of oxygen flowing out so as to control the reaction degree in the reaction kettle (2), the output end of the reaction kettle (2) is connected with a gas filter (9) through a pipeline, the gas filter (9) is suitable for filtering gas generated by reaction in the reaction kettle (2), so as to prevent other gas generated in the reaction kettle (2) from generating side effects on the growth of embryo eggs, the output end of the gas filter (9) is connected with the culture cabin (4) through a pipeline, a first control screen (101) is fixedly installed on the right side of the culture box (1), the first control screen (101) controls the environment in the culture box (1) through a plc system, a feeding pipe (102) is installed on the right side of the culture box (1) and below the first control screen (101), a sliding groove (103) is formed in the inner surface of the culture box (1), a sliding plate (104) is fixedly installed on the side surface of the culture cabin (4), the inside fixed mounting of spout (103) has guide rail (105), cultivate the inboard of cabin (4) and be located the top of slide (104) and seted up oxygen entry (106), oxygen entry (106) are evenly arranged at the internal surface of cultivating cabin (4), the last fixed surface who cultivates cabin (4) installs cylinder (107), the output of cylinder (107) and the last fixed surface who cuts a hole mechanism (7) are connected, the electrical input of cylinder (107) and control panel (101) electric connection.
Fixed surface of reation kettle (2) installs No. two display screens (201), No. two display screens (201) are suitable for the inside reaction degree of control accurate control reation kettle (2), and reation kettle's (2) upper surface covers has upper cover (202), and the upper surface mounting of upper cover (202) has raw materials pipe (203) and No. two raw materials pipe (204), and raw materials pipe (203) and No. two raw materials pipe (204) are suitable for the raw materials of interpolation manufacturing oxygen.
An antibiotic output tube (301) is fixedly installed at the output end of the antibiotic storage box (3), slots (501) are formed in two sides of the storage tray (5), the inner surfaces of the slots (501) are connected with a sliding plate (104) in an inserting mode, a fixing groove (502) is formed in the upper surface of the storage tray (5), a protection pad (503) is fixedly installed on the inner surface of the fixing groove (502), the diameter of the fixing groove (502) is matched with the transverse diameter of the embryonated egg, so that the embryonated egg can be conveniently taken out and vertically fixed, the hole drilling mechanism (7) comprises a connecting plate, a spray head (701) is fixedly installed on the lower surface of the connecting plate, a plurality of rows of insertion tubes (702) are fixedly installed on the lower surface of the spray head (701), the plurality of rows of insertion tubes (702) are arranged in a certain radian mode, the radian formed by the plurality of rows of insertion tubes (702, make inside even with external contact of embryo egg, and to the force evenly distributed of embryo egg shell application, prevent that embryo egg shell from breaking, the dorsal part fixed mounting of connecting plate has lantern ring (703), the internal surface and the guide rail (105) activity of lantern ring (703) are cup jointed, the upper surface and cylinder (107) fixed connection of connecting plate, injection opening (704) have been seted up on the right side of connecting plate, the output and intubate (702) of injection opening (704) switch on, the input and pan feeding pipe (102) of injection opening (704) switch on, under the promotion of cylinder (107) like this, vertical lift can be stabilized in chisel hole mechanism (7) to embryo egg and evenly inject the operation.
By this embodiment it is achieved that: the radian formed by the multiple rows of insertion tubes (702) is the same as the radian formed by the outer surface of the embryo egg, so that the punching density of the upper surface of the embryo egg is the same, the contact between the inside of the embryo egg and the outside is uniform, the force applied to the embryo egg shell is uniformly distributed, the embryo egg shell is prevented from being broken, and meanwhile, the injected antibiotics are uniformly contacted with the inside of the embryo egg.
Example 2
As shown in FIGS. 1-5, a method for producing an antioxidant in an embryo egg comprises the following steps:
the method comprises the following steps: cleaning fertilized eggs which are just born, vertically placing the fertilized eggs in fixing grooves (502) on the upper surface of a storage tray (5), placing one fertilized egg in each fixing groove (502), movably inserting and connecting a slot (501) on the side surface of the storage tray (5) with a sliding plate (104) on the inner surface of a culture cabin (4), placing the storage tray (5) in the culture cabin (4), and culturing the embryo eggs in a culture box (1) under the conditions of constant temperature and humidity and constant oxygen concentration for 7-8 days, and then performing a second step;
step two: after the first step is completed, the control screen (101) controls the cylinder (107) to be pressed down integrally, the lantern ring (703) slides on the outer surface of the guide rail (105) in the sliding groove (103), the spray head (701) drives the insertion pipes (702) to chisel holes on the upper surface of the fertilized egg, the insertion pipes (702) are arranged in a certain radian, and the radian formed by the insertion pipes (702) is the same as that formed by the outer surface of the embryo egg, so that the hole chiseling density on the upper surface of the embryo egg is the same, the inside of the embryo egg is uniformly contacted with the outside, the force applied to the embryo egg shell is uniformly distributed, and the embryo egg shell is prevented from being broken;
step three: the fertilized eggs are influenced by the external environment to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products so as to resist the influence of the external environment;
step four: the first control screen (101) controls the cylinder (107) to be pressed down integrally again, the lantern ring (703) slides on the outer surface of the guide rail (105) inside the sliding chute (103), and antibiotics inside the antibiotic storage tank (3) are injected into the fertilized eggs through the antibiotic output pipe (301), the antibiotic inlet pipe (102) and the antibiotic inlet opening (704) and through the intubation tube (702) to generate antioxidant ingredients;
step five: after the fourth step is finished, the second display screen (201) controls the reaction in the reaction kettle (2) to be rapidly carried out, a large amount of oxygen enters the culture cabin (4) through the gas filter (9), the flow valve (10) and the oxygen inlet (106) to enable the oxygen concentration in the culture cabin (4) to reach ninety percent, so that the embryo eggs generate a large amount of antioxidant components to resist oxidation, and after oxygen enrichment supply is carried out for one day, the oxygen concentration in the culture cabin (4) reaches the normal level of the outside air;
step six: after the fifth step is finished, the oxygen concentration in the culture cabin (4) is continuously reduced, a large amount of antioxidant substances are also generated due to the biological inertia effect, the content of the antioxidant ingredients in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin (4) to extract the antioxidant peptides.
The optimal hatching condition of the fertilized eggs is that the fertilized eggs are placed in a hatching box with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for hatching for 6-7d, thus facilitating the synthesis and secretion of antioxidant substances in the embryo eggs.
The antibiotic contains a large amount of components that inhibit the secretion of acidic and neutral proteases to inhibit the secretion of acidic and neutral proteases, thereby promoting the growth of alkaline proteases.
By this embodiment it is achieved that: according to the invention, by utilizing the inertia movement law of life movement, the radian formed by a plurality of rows of insertion tubes (702) is the same as the radian formed by the outer surface of an embryo egg, so that the hole punching density on the upper surface of the embryo egg is the same, the inner part of the embryo egg is uniformly contacted with the outside, the force applied to the egg shell of the embryo is uniformly distributed, the egg shell of the embryo is prevented from being broken, and the injected antibiotic is uniformly contacted with the inner part of the embryo egg, so that the fertilized egg starts to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products due to the influence of the external environment, and then the secretion of acid protease and neutral protease is inhibited by the created antibiotic inhibition environment, so that the growth of alkaline protease is promoted, the created oxygen-enriched environment is favorable for the generation of antioxidant active ingredient substances, and the antioxidant ingredients in the embryo egg can.
The invention is a production method for improving antioxidant components in embryo eggs, when in preparation, firstly, just born fertilized eggs are cleaned and vertically placed in the fixed grooves (502) on the upper surface of a storage tray (5), a fertilized egg is placed in each fixed groove (502), the slot (501) on the side surface of the storage tray (5) is movably inserted into the sliding plate (104) on the inner surface of a culture cabin (4), the storage tray (5) is placed in the culture cabin (4), the embryo eggs are cultured for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration in a culture box (1), a first control screen (101) controls the whole cylinder (107) to be pressed downwards, a lantern ring (703) slides on the outer surface of a guide rail (105) in the sliding groove (103), and a nozzle (701) drives an insertion pipe (702) to chisel the upper surface of the fertilized eggs, the multiple rows of insertion tubes (702) are arranged in a certain radian, the radian formed by the multiple rows of insertion tubes (702) is the same as the radian formed by the outer surface of an embryo egg, so that the hole drilling density on the upper surface of the embryo egg is the same, the inside of the embryo egg is uniformly contacted with the outside, the force applied to the egg shell of the embryo is uniformly distributed, the egg shell of the embryo is prevented from being broken, fertilized eggs start to secrete and manufacture a large number of proteases, antioxidant polypeptides and enzymolysis products under the influence of the external environment so as to resist the influence of the external environment, the control screen (101) controls the cylinder (107) to be pressed down integrally again, the lantern ring (703) slides on the outer surface of the guide rail (105) inside the sliding chute (103), antibiotics inside the antibiotic storage tank (3) are injected into the fertilized eggs through the antibiotic output tube (301), the feed tube (102) and the injection port (704) so as to generate antioxidant ingredients, the second display screen (201) controls the reaction inside the reaction kettle (2) to be rapidly carried out, a large amount of oxygen enters the inside of the culture cabin (4) through the gas filter (9), the flow valve (10) and the oxygen inlet (106), so that the oxygen concentration inside the culture cabin (4) reaches ninety percent, the embryo eggs generate a large amount of antioxidant ingredients to resist oxidation, after the oxygen enrichment is supplied for one day, the oxygen concentration inside the culture cabin (4) reaches the normal level of the outside air, the oxygen concentration inside the culture cabin (4) is continuously reduced, due to the biological inertia effect, a large amount of antioxidant substances are required to be generated, at the moment, the content of the antioxidant ingredients in the embryo eggs is highest, and the embryo eggs can be taken out from the culture cabin (4) to extract antioxidant peptides.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (7)
1. A production method for improving the antioxidant ingredient in an embryo egg is characterized in that: the method comprises the following steps:
the method comprises the following steps: cleaning the fertilized eggs which are just born, vertically placing the fertilized eggs in the fixed grooves on the upper surface of the storage tray, placing one fertilized egg in each fixed groove, movably inserting the slots on the side surface of the storage tray into the sliding plates on the inner surface of the culture cabin, placing the storage tray in the culture cabin, and culturing the embryo eggs in the incubator for 7-8 days under the conditions of constant temperature and humidity and constant oxygen concentration;
step two: after the step one is finished, the control screen I controls the cylinder to be pressed down integrally, the lantern ring slides on the outer surface of the guide rail in the sliding groove, the spray head drives the insertion tubes to chisel the upper surface of the fertilized egg, the multiple rows of insertion tubes are arranged in a certain radian, the radian formed by the multiple rows of insertion tubes is the same as the radian formed by the outer surface of the embryo egg, thus the hole-chiseling density on the upper surface of the embryo egg is the same, the inside of the embryo egg is in uniform contact with the outside, the force applied to the embryo egg shell is uniformly distributed, and the embryo egg shell is prevented from being broken;
step three: the fertilized eggs are influenced by the external environment to secrete and produce a large amount of various proteases, antioxidant polypeptides and enzymolysis products so as to resist the influence of the external environment;
step four: the first control screen controls the cylinder to be pressed down integrally again, the lantern ring slides on the outer surface of the guide rail in the sliding groove, and antibiotics in the antibiotic storage box are injected into the fertilized eggs through the antibiotic output pipe, the feed pipe and the injection port and the intubation tube to generate antioxidant ingredients;
step five: after the fourth step is finished, the second display screen controls the reaction in the reaction kettle to be rapidly carried out, a large amount of oxygen enters the culture cabin through the gas filter, the flow valve and the oxygen inlet, so that the oxygen concentration in the culture cabin reaches ninety percent, the embryo eggs generate a large amount of antioxidant components to resist oxidation, and after oxygen enrichment supply is carried out for one day, the oxygen concentration in the culture cabin reaches the normal level of the outside air;
step six: and after the fifth step is finished, continuously reducing the oxygen concentration in the culture cabin, and generating a large amount of antioxidant substances due to the biological inertia effect, wherein the content of the antioxidant ingredients in the embryo eggs is the highest, and the embryo eggs can be taken out from the culture cabin for extracting the antioxidant peptides.
2. The method of claim 1, wherein the step of increasing the antioxidant content of the egg comprises the steps of: still include the apparatus for producing that improves anti oxidation composition in embryo's egg, the device includes incubator and reation kettle, reation kettle fixed mounting is at the upper surface of incubator, the dorsal part fixed mounting of incubator has the antibiotic bin, the antibiotic in the antibiotic bin has the chemical characteristic who restraines acidity and neutral protease growth, the cultivation cabin has been seted up to the inside of incubator, the middle part of incubator is provided with deposits the dish, the inside of cultivation cabin just is located and is provided with chisel hole mechanism directly over depositing the dish, reation kettle's output has flow controller through gas transmission pipe connection.
3. The apparatus for increasing antioxidant content of an egg as claimed in claim 2, wherein: the flow controller is suitable for controlling the flow of oxygen flowing out to control the reaction degree in the reaction kettle, the output end of the reaction kettle is connected with a gas filter through a pipeline, the gas filter is suitable for filtering gas generated by reaction in the reaction kettle to prevent other gas generated in the reaction kettle from generating side effects on the growth of embryonated eggs, the output end of the gas filter is connected with the culture cabin through a pipeline, a control screen is fixedly arranged on the right side of the culture cabin, the control screen controls the environment in the culture cabin through a plc system, a feeding pipe is arranged on the right side of the culture cabin and below the control screen, a sliding groove is formed in the inner surface of the culture cabin, a sliding plate is fixedly arranged on the side surface of the culture cabin, a guide rail is fixedly arranged in the sliding groove, an oxygen inlet is formed in the inner side of the culture cabin and above the sliding plate, the oxygen entry is evenly arranged at the internal surface of cultivateing the cabin, the last fixed surface of cultivateing the cabin installs the cylinder, the output of cylinder and the last fixed surface of drilling mechanism are connected, the electrical property input and the control panel electric connection of cylinder.
4. The apparatus for increasing antioxidant content of in-embryo eggs as claimed in claim 3, wherein: the utility model discloses a control system for the oxygen production of reaction kettle, including reation kettle, outer fixed surface installs No. two display screens of reation kettle, No. two display screens are suitable for the inside reaction degree of control accurate control reation kettle, reation kettle's upper surface lid has closed the upper cover, the upper surface mounting of upper cover has raw materials pipe and No. two raw materials pipes, raw materials pipe and No. two raw materials pipes are suitable for adding the raw materials of making oxygen.
5. The apparatus for increasing antioxidant content of egg embryo according to claim 4, wherein: the output end of the antibiotic storage box is fixedly provided with an antibiotic output tube, the two sides of the storage tray are provided with slots, the inner surfaces of the slots are spliced with the sliding plate, the upper surface of the storage tray is provided with a fixed groove, the inner surface of the fixed groove is fixedly provided with a protection pad, the diameter of the fixed groove is matched with the transverse diameter of the embryonated egg, so that the embryonated egg can be conveniently taken out and vertically fixed, the hole drilling mechanism comprises a connecting plate, the lower surface of the connecting plate is fixedly provided with a spray head, the lower surface of the spray head is fixedly provided with a plurality of rows of insertion tubes, the plurality of rows of insertion tubes are arranged in a certain radian, the radian formed by the plurality of rows of insertion tubes is the same as the radian formed by the outer surface of the embryonated egg, thus the hole drilling density on the upper surface of the embryonated egg is the same, prevent that embryo's eggshell from breaking, the dorsal part fixed mounting of connecting plate has the lantern ring, the internal surface and the guide rail activity of the lantern ring cup joint, the upper surface and the cylinder fixed connection of connecting plate, the filling opening has been seted up on the right side of connecting plate, the output and the intubate of filling opening switch on, the input and the pan feeding pipe of filling opening switch on, and under the promotion of cylinder, the vertical lift of chisel hole mechanism can be stabilized like this and the operation is chisel hole and evenly injected to embryo's egg.
6. The method of claim 1, wherein the step of increasing the antioxidant content of the egg comprises the steps of: the optimal hatching condition of the fertilized eggs is that the fertilized eggs are placed in a hatching box with the hatching temperature of 38-39 ℃, the oxygen concentration of 40-50% and the relative humidity of 50-55% for hatching for 6-7d, thus facilitating the synthesis and secretion of antioxidant substances in the embryo eggs.
7. The method of claim 1, wherein the step of increasing the antioxidant content of the egg comprises the steps of: the antibiotic contains a large amount of components for inhibiting the secretion of acidic protease and neutral protease to inhibit the secretion of acidic protease and neutral protease, thereby promoting the growth of alkaline protease.
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