CN112608955A - Process for extracting glucose from starch sugar solution - Google Patents
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- CN112608955A CN112608955A CN202011587951.5A CN202011587951A CN112608955A CN 112608955 A CN112608955 A CN 112608955A CN 202011587951 A CN202011587951 A CN 202011587951A CN 112608955 A CN112608955 A CN 112608955A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
- C07H3/02—Monosaccharides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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Abstract
The invention provides a process for extracting glucose from a starch sugar solution, and relates to the field of glucose preparation. The process for extracting the glucose from the starch-based sugar solution comprises the following processes of micro-dilution, crystallization, hydrolysis, decolorization and drying, wherein the micro-dilution comprises the following steps: s 1: diluting with distilled water to obtain starch sugar solution; s 2: stirring, namely fully stirring the starch sugar solution and distilled water to form a solution a; the crystallization process comprises the following steps: s 3: crystallizing, adding a proper amount of sucrase and a 40% sodium bisulfite solution into the solution a, fully stirring to form a solution b, and crystallizing the solution b; s 4: and (3) filtering: and filtering the solution b precipitate to obtain a precipitate c. The processing technology comprises the following processes of micro-dilution, crystallization, hydrolysis, decolorization and drying, and has the advantages of short processing time, high product yield, sufficient raw material extraction and low manufacturing cost.
Description
Technical Field
The invention relates to the technical field of glucose preparation, in particular to a process for extracting glucose from starch sugar solution.
Background
The starch sugar is prepared from grain containing starch, potatoes and the like serving as raw materials by an acid method, an acid enzyme method or an enzyme method, and comprises maltose, glucose, high fructose syrup and the like, which are collectively called starch sugar. Starch sugar has a long history in our country, and sugar is mentioned in "the Chinese treatise" of more than 500 A.C. and the method of making sugar with rice is described in detail. For a long time, the production and consumption of sugar in China mainly adopt cane sugar and the auxiliary adopt starch sugar, and the nation vigorously fosters and popularizes the starch sugar in recent years. In the later stage of 'nine five', due to the technical progress of the starch sugar industry, the yield and the quality are improved, and the material consumption is reduced; the enterprise is intensively operated in scale, the cost of the starch sugar product is greatly reduced, and the starch sugar market is gradually expanded to become an important supplement of the sugar market. The starch sugar has wide consumption field and large consumption quantity, is a support product for deep processing of starch, is widely applied to various industries such as food, medicine, paper making and the like for a long time, and in recent years, along with the development of deep processing of corn, food industry, the progress of biotechnology such as enzyme preparation and the like and the change of consumption structure of people, the starch sugar industry in China has remarkable development, and is developed towards multiple varieties, individuation, specialization and large-scale development, the yield is greatly increased, and the variety structure is increasingly perfect. Glucose, also known as corn sugar, or even simply glucose, is the most widely distributed and important monosaccharide in nature, a polyhydroxyl aldehyde. Pure glucose is colorless crystals, has sweet taste but is not as sweet as sucrose, and is preferably soluble in water, slightly soluble in ethanol, and insoluble in diethyl ether. Aqueous solutions rotate optically to the right, so they are also called "dextrose". Glucose plays an important role in the field of biology, being an energy source and a metabolic intermediate of living cells. Meanwhile, glucose is widely applied in the fields of candy manufacturing industry and medicine.
The applicant finds that a Chinese patent discloses an automatic pot washing device through retrieval when applying the invention, the application number of the automatic pot washing device is 201510267112.8, the patent belongs to the field of glucose processing technology, and particularly relates to a technology for extracting glucose from starch saccharification liquid. The process comprises a concentration process, a decolorization process, a crystallization separation process and a drying process which are sequentially carried out, and compared with the prior art, the process has the advantages and positive effects that the process for extracting the glucose from the starch saccharification liquid is simple and low in energy consumption by changing the traditional production process and utilizing novel equipment, and the produced glucose is high in quality and free of impurities. The processing of technology is carried out by adopting more advanced equipment, the manufacturing cost of products is higher, primary waste cannot be fully utilized, and a large amount of products are wasted.
When the applicant applies for the invention, through search, the inventor finds that a Chinese patent discloses an automatic pot washer, the application number of which is '201410583586.9', and the patent comprises the following steps: (1) adding water into starch for size mixing to obtain starch slurry; (2) adding high-temperature resistant amylase into the starch slurry, and performing primary injection liquefaction and secondary injection liquefaction to obtain liquefied liquid; (3) adding saccharifying enzyme into the liquefied liquid for saccharification to prepare saccharified liquid; (4) decolorizing the saccharified solution, filtering, ion-exchange, and vacuum concentrating to obtain glucose concentrated solution; (5) carrying out high-temperature vacuum melt polycondensation reaction on the glucose concentrated solution to obtain a polydextrose crude product; (6) decoloring, ion-crossing and carrying out chromatographic separation on the polydextrose crude product to obtain polydextrose liquid; (7) and concentrating and drying the polydextrose solution to obtain polydextrose. The invention prepares the high conversion rate glucose concentrated solution as the raw material to produce the polydextrose by two times of jetting liquefaction and saccharification processes, compared with the traditional process, the production cost is greatly saved, the energy consumption is reduced, and the produced polydextrose has better quality. The processing time is long, and the product yield efficiency is low.
Therefore, a process for extracting glucose from starch sugar solution is provided.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides the process for extracting the glucose from the starch sugar solution, and solves the problems of higher manufacturing cost, insufficient raw material utilization, long processing time consumption and low product yield in the prior art.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a process for extracting glucose from starch sugar solution comprises the following steps of micro-dilution, crystallization, hydrolysis, decolorization and drying, wherein the micro-dilution comprises the following steps:
s 1: diluting with distilled water to obtain starch sugar solution;
s 2: stirring, namely fully stirring the starch sugar solution and distilled water to form a solution a;
the crystallization process comprises the following steps:
s 3: crystallizing, adding a proper amount of sucrase and a 40% sodium bisulfite solution into the solution a, fully stirring to form a solution b, and crystallizing the solution b;
s 4: and (3) filtering: filtering the solution b precipitate to obtain a precipitate c;
the hydrolysis process comprises the following steps:
s 5: hydrolyzing, namely placing the precipitate c in a generating vessel, and adding distilled water to fully stir to form a solution d;
s 6: adding enzyme, namely adding a proper amount of maltase into the solution d, and fully stirring to form a solution e;
s 7: filtering the solution e with a semipermeable membrane to obtain a solution f;
the decoloring process comprises the following steps:
s 8: decoloring, namely decoloring the solution f by using activated carbon to obtain a solution g;
s9, exchanging anions and cations, and exchanging anions and cations of the solution g to obtain a solution h;
the drying process comprises the following steps:
s 10: the solution h is dried by means of a dryer.
Preferably, the mass ratio of the starch sugar solution to the distilled water in the step s1 is 1/3.
Preferably, the solution b is heated and the steps s3 and s4 are repeated to obtain the precipitate c.
Preferably, the mass ratio of the precipitate c to the distilled water in the step s5 is 1/5.
Preferably, the semi-permeable membrane filtration of the solution e in step s7 is repeated several times, and the work is performed by appropriately replacing the new semi-permeable membrane.
Preferably, the solution f in the step s8 is decolorized by multiple groups of activated carbon.
The working principle is as follows: a process for extracting glucose from starch sugar solution comprises the following steps of micro-dilution, crystallization, hydrolysis, decolorization and drying, wherein the micro-dilution comprises the following steps:
s 1: diluting with distilled water to obtain starch sugar solution;
s 2: stirring, namely fully stirring the starch sugar solution and distilled water to form a solution a;
the crystallization process comprises the following steps:
s 3: crystallizing, adding a proper amount of sucrase and a 40% sodium bisulfite solution into the solution a, fully stirring to form a solution b, and crystallizing the solution b;
s 4: and (3) filtering: filtering the solution b precipitate to obtain a precipitate c;
the hydrolysis process comprises the following steps:
s 5: hydrolyzing, namely placing the precipitate c in a generating vessel, and adding distilled water to fully stir to form a solution d;
s 6: adding enzyme, namely adding a proper amount of maltase into the solution d, and fully stirring to form a solution e;
s 7: filtering the solution e with a semipermeable membrane to obtain a solution f;
the decoloring process comprises the following steps:
s 8: decoloring, namely decoloring the solution f by using activated carbon to obtain a solution g;
s9, exchanging anions and cations, and exchanging anions and cations of the solution g to obtain a solution h;
the drying process comprises the following steps:
s 10: the solution h is dried by means of a dryer.
In step s1, the mass ratio of starch sugar solution to distilled water is 1/3.
Solution b was heated and steps s3 and s4 were repeated to obtain precipitate c.
The mass ratio of precipitate c to distilled water in step s5 was 1/5.
The filtration of the semipermeable membrane of the solution e in step s7 is repeated several times and works by appropriately replacing the semipermeable membrane with a new one.
And (5) decoloring the solution f by using multiple groups of activated carbon in the step s 8.
(III) advantageous effects
The invention provides a process for extracting glucose from starch sugar solution. The method has the following beneficial effects:
1. the invention provides a process for extracting glucose from a starch sugar solution, which comprises the steps of adding a proper amount of sucrase and a 40% sodium bisulfite solution into a solution a in the crystallization process, fully stirring to form a solution b, wherein the solution b is a mixed solution of fructose, glucose, sucrose, maltose and the sodium bisulfite solution, crystallizing the solution b, heating the solution b, repeating the steps s3 and s4 to obtain a precipitate c, and the precipitate c is a mixed crystal of glucose and maltose, so that the extraction rate of the glucose and the maltose is improved; meanwhile, in the step 7, the solution e is filtered through the semipermeable membrane to obtain a solution f, the solution e is a mixed solution of glucose and maltose, the solution f is a glucose solution, the filtration of the semipermeable membrane of the solution e in the step s7 is repeated for multiple times, the appropriate replacement of the semipermeable membrane is adopted to work, the extraction rate of the glucose is improved, the raw materials are fully extracted, and the manufacturing cost is low.
2. The invention provides a process for extracting glucose from starch sugar liquid, which comprises the following steps of micro-dilution, crystallization, hydrolysis, decolorization and drying, and has the advantages of short processing time and high product yield.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example (b):
the embodiment of the invention provides a process for extracting glucose from starch sugar liquid, which comprises the following processes of micro-dilution, crystallization, hydrolysis, decolorization and drying, wherein the micro-dilution comprises the following steps:
s 1: diluting with distilled water to obtain starch sugar solution;
s 2: stirring, namely fully stirring the starch sugar solution and distilled water to form a solution a;
the crystallization process comprises the following steps:
s 3: crystallizing, adding a proper amount of sucrase and a 40% sodium bisulfite solution into the solution a, fully stirring to form a solution b, and crystallizing the solution b;
s 4: and (3) filtering: filtering the solution b precipitate to obtain a precipitate c;
the hydrolysis process comprises the following steps:
s 5: hydrolyzing, namely placing the precipitate c in a generating vessel, and adding distilled water to fully stir to form a solution d;
s 6: adding enzyme, namely adding a proper amount of maltase into the solution d, and fully stirring to form a solution e;
s 7: filtering the solution e with a semipermeable membrane to obtain a solution f;
the decoloring process comprises the following steps:
s 8: decoloring, namely decoloring the solution f by using activated carbon to obtain a solution g;
s9, exchanging anions and cations, and exchanging anions and cations of the solution g to obtain a solution h;
the drying process comprises the following steps:
s 10: the solution h is dried by means of a dryer.
The starch sugar mainly comprises fructose, glucose, sucrose and maltose;
the solution a is a mixed solution of fructose, glucose, sucrose and maltose;
the solution b is a mixed solution of fructose, glucose, sucrose, maltose and sodium bisulfite;
the precipitate c is glucose and maltose crystals, and contains a small amount of metallic sodium;
the solution d is a mixed solution of glucose and maltose, and contains a small amount of metallic sodium;
the solution e is a mixed solution of glucose and maltose, and contains a small amount of metallic sodium;
the solution f is glucose solution and contains a small amount of metallic sodium;
the solution g is glucose solution and contains a small amount of metallic sodium;
the solution h is glucose solution;
in step s1, the mass ratio of starch sugar solution to distilled water is 1/3.
Solution b was heated and steps s3 and s4 were repeated to obtain precipitate c.
The mass ratio of precipitate c to distilled water in step s5 was 1/5.
The filtration of the semipermeable membrane of the solution e in step s7 is repeated several times and works by appropriately replacing the semipermeable membrane with a new one.
And (5) decoloring the solution f by using multiple groups of activated carbon in the step s 8.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The process for extracting the glucose from the starch sugar solution is characterized by comprising the following processes of micro-dilution, crystallization, hydrolysis, decolorization and drying, wherein the micro-dilution comprises the following steps:
s 1: diluting with distilled water to obtain starch sugar solution;
s 2: stirring, namely fully stirring the starch sugar solution and distilled water to form a solution a;
the crystallization process comprises the following steps:
s 3: crystallizing, adding a proper amount of sucrase and a 40% sodium bisulfite solution into the solution a, fully stirring to form a solution b, and crystallizing the solution b;
s 4: and (3) filtering: filtering the solution b precipitate to obtain a precipitate c;
the hydrolysis process comprises the following steps:
s 5: hydrolyzing, namely placing the precipitate c in a generating vessel, and adding distilled water to fully stir to form a solution d;
s 6: adding enzyme, namely adding a proper amount of maltase into the solution d, and fully stirring to form a solution e;
s 7: filtering the solution e with a semipermeable membrane to obtain a solution f;
the decoloring process comprises the following steps:
s 8: decoloring, namely decoloring the solution f by using activated carbon to obtain a solution g;
s9, exchanging anions and cations, and exchanging anions and cations of the solution g to obtain a solution h;
the drying process comprises the following steps:
s 10: the solution h is dried by means of a dryer.
2. The process for extracting glucose from a starch sugar solution according to claim 1, wherein: in the step s1, the mass ratio of the starch sugar solution to the distilled water is 1/3.
3. The process for extracting glucose from a starch sugar solution according to claim 1, wherein: the solution b is heated and steps s3 and s4 are repeated to obtain a precipitate c.
4. The process for extracting glucose from a starch sugar solution according to claim 1, wherein: the mass ratio of the precipitate c to the distilled water in the step s5 is 1/5.
5. The process for extracting glucose from a starch sugar solution according to claim 1, wherein: the semi-permeable membrane filtration of the solution e in step s7 is repeated several times and the work is done with the appropriate replacement of the semi-permeable membrane.
6. The process for extracting glucose from a starch sugar solution according to claim 1, wherein: and (5) decoloring the solution f in the step s8 by using multiple groups of activated carbon.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011587951.5A CN112608955A (en) | 2020-12-29 | 2020-12-29 | Process for extracting glucose from starch sugar solution |
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| CN202011587951.5A CN112608955A (en) | 2020-12-29 | 2020-12-29 | Process for extracting glucose from starch sugar solution |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES536435A0 (en) * | 1984-10-02 | 1985-07-01 | Uop Inc | A PROCEDURE FOR SELECTIVELY OBTAINING A SUGAR, WHICH IS GLUCOSE OR MALTOSE, FROM DILUTED STARCH |
| CN101724718A (en) * | 2002-06-27 | 2010-06-09 | 丹尼斯克有限公司 | crystallization of sugars |
| CN104356251A (en) * | 2014-10-27 | 2015-02-18 | 山东广博生物技术服务有限公司 | Method for producing polydextrose by taking starch as raw material |
| CN105483181A (en) * | 2015-12-28 | 2016-04-13 | 河南飞天农业开发股份有限公司 | Preparation method of high-purity glucose |
| CN105821095A (en) * | 2015-09-14 | 2016-08-03 | 呼伦贝尔东北阜丰生物科技有限公司 | Optimization method for crystallization of glucose |
-
2020
- 2020-12-29 CN CN202011587951.5A patent/CN112608955A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES536435A0 (en) * | 1984-10-02 | 1985-07-01 | Uop Inc | A PROCEDURE FOR SELECTIVELY OBTAINING A SUGAR, WHICH IS GLUCOSE OR MALTOSE, FROM DILUTED STARCH |
| CN101724718A (en) * | 2002-06-27 | 2010-06-09 | 丹尼斯克有限公司 | crystallization of sugars |
| CN104356251A (en) * | 2014-10-27 | 2015-02-18 | 山东广博生物技术服务有限公司 | Method for producing polydextrose by taking starch as raw material |
| CN105821095A (en) * | 2015-09-14 | 2016-08-03 | 呼伦贝尔东北阜丰生物科技有限公司 | Optimization method for crystallization of glucose |
| CN105483181A (en) * | 2015-12-28 | 2016-04-13 | 河南飞天农业开发股份有限公司 | Preparation method of high-purity glucose |
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