CN112608870A - Probiotics starter based on material source protein - Google Patents
Probiotics starter based on material source protein Download PDFInfo
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Abstract
The invention relates to a probiotic starter based on a biogenic protein, belonging to the technical field of probiotic preparation, and the probiotic starter comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; manganese sulfate 0.29g/L and agar 18 g/L.
Description
Technical Field
The invention relates to the technical field of probiotic preparation, and in particular relates to a probiotic starter based on a biogenic protein.
Background
Probiotics is a kind of active microorganisms beneficial to a host, and is a general term for active beneficial microorganisms which are planted in the intestinal tract and the reproductive system of a human body and can generate definite health efficacy so as to improve the micro-ecological balance of the host and play beneficial roles. The beneficial bacteria or fungi in human bodies and animal bodies are mainly as follows: clostridium butyricum, lactobacillus, bifidobacterium, actinomycete, lactobacillus plantarum, etc. The metabolites of probiotics range from simple short chain fatty acids to organic acids with complex structures such as folic acid, orotic acid and the like which are necessary for human bodies, and the compounds can lower the pH value of the digestive tract, form an acidic environment and regulate a series of physiological processes of a host. Various enzymes released by probiotics in the intestinal cavity can play a synergistic role in the digestion process and relieve the symptom of partial intestinal malabsorption. In addition, antibacterial substances such as bacteriocin and the like generated by the probiotics can improve the antibacterial effect of the probiotics in vivo and increase the application potential of the probiotics as a biological treatment method.
The probiotics play a role in the beneficial effect of the probiotics starter culture, the existing preparation method of the probiotics starter culture has the disadvantages of complex production process, strict requirement on operating conditions, higher production cost, and inconvenience for operation and use of preparation personnel because multiple processes are independent in different devices. Based on the probiotic fermentation agent, the invention designs the probiotic fermentation agent based on the source protein.
Disclosure of Invention
Solves the technical problem
In view of the above-mentioned disadvantages of the prior art, the present invention provides a probiotic starter based on a protein of origin.
Technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme:
a probiotic starter based on a biogenic protein, which comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; manganese sulfate 0.29g/L and agar 18 g/L.
A preparation process of a probiotic starter based on a source protein further comprises the following preparation process:
s1: taking lactobacillus plantarum as a fermentation strain, putting the lactobacillus plantarum into a culture medium base solution used by an activation culture assembly, and introducing oxygen for culturing for 16-28h at the temperature of 26-30 ℃;
s2: carrying out centrifugal separation on the activated and cultured thalli by a centrifugal separation component at the rotating speed of 5000-;
s3: dissolving the separated thallus by using a freeze-drying agent;
s4: freeze-drying at-55 deg.C to-35 deg.C to obtain Lactobacillus plantarum freeze-dried strain.
A probiotic starter based on a source protein comprises an incubator, wherein a culture area and a freeze-drying area are arranged inside the incubator, a separation groove is arranged between the culture area and the freeze-drying area, a partition plate is arranged inside the separation groove, an activation culture assembly is arranged above the partition plate, and the activation culture assembly comprises a culture tube, an outlet, a solenoid valve, a heater, a vertical rod, a transverse plate, a through hole, a telescopic outer cylinder, a sliding groove, a screw rod, a sliding block, a connecting bearing, a telescopic inner cylinder, an air hole, a stirring rod, an air tube, a driven belt pulley, a servo motor, an output shaft, a main belt pulley and a belt;
the culture tube is arranged on the partition plate, the bottom end of the culture tube penetrates through the bottom end of the partition plate, a culture substrate is arranged in the culture tube, the outlet is arranged at the bottom end of the culture tube, the electromagnetic valve is arranged in the outlet, the heater is symmetrically arranged at the center of the side wall of the culture area, the vertical rods are symmetrically fixed on two sides of the center of the top end of the culture box, the transverse plate is fixed at the top end of the vertical rods, the through holes are arranged at the center of the transverse plate, the telescopic outer cylinder is fixed at the center of the top end of the transverse plate, the sliding grooves are symmetrically arranged on the inner wall of the telescopic outer cylinder, the screw rod rotates at the top end and the bottom end of the sliding grooves and penetrates through the telescopic outer cylinder, the sliding block slides in the sliding grooves, the screw rod threads penetrate through the sliding block, the air pipe is communicated with the top end of the telescopic inner cylinder and movably penetrates through the top end of the telescopic outer cylinder, the air pipe is externally connected with an air supply device, the driven belt pulley is arranged at the top end of the screw rod, the servo motor is arranged at the top end of the telescopic outer cylinder, the main belt pulley is connected with the output shaft, and the belt transmission is arranged between the two groups of driven belt pulleys and the main belt pulley;
a centrifugal separation assembly is arranged in the incubator and comprises a rotating bearing, a mounting groove, a centrifugal motor, a centrifugal rotating shaft, a centrifugal belt wheel, a driving wheel, a centrifugal belt, a through groove, an annular groove, a cover plate and a handle;
the rotating bearing is embedded in the center of the partition plate, the bottom end of the culture tube penetrates through the bottom end of the partition plate through the rotating bearing, the mounting groove is formed in one side of the top end of the incubator in a culture area, the centrifugal motor is mounted in the mounting groove, the centrifugal belt wheel is connected to the end portion of the centrifugal rotating shaft, the driving wheel is mounted at the top end of the side wall of the culture tube, the through groove is formed in the side wall of the mounting groove, the centrifugal belt penetrates through the through groove to be driven between the driving wheel and the centrifugal belt wheel, the annular groove is formed in the edge of the top end of the mounting groove, the cover plate is;
the side wall of the incubator is provided with an observation area and a control panel, a refrigerator is arranged on the inner wall of the freeze-drying area, an opening and closing opening is formed in the outer wall of the freeze-drying area, opening and closing chutes are symmetrically formed in two sides of the opening and closing opening, an opening and closing slider slides in the opening and closing chutes, an opening and closing plate is fixed on the side face of the opening and closing slider, a magnetic plate is fixed on the side wall of the incubator, an installation chute is formed in the bottom end of the freeze-drying area, a stop block is matched with the center of the bottom end of the opening and closing plate, an installation slider slides in the installation chute, a freeze-drying box is installed;
the culture area is characterized in that a through groove is formed in the side wall of the culture area, a sealing plate is arranged in the through groove, an auxiliary handle is arranged at the center of the side face of the sealing plate, and a semicircular hole is formed in the other side face of the sealing plate
Furthermore, the culture area and the freeze-drying area are the same in size, the length of the separation groove is larger than the length of the culture area in the direction, and the width of the separation groove is equal to the length of the culture area in the direction.
Further, the culture tube bottom is less than cultivates the district top, the diameter of export is less than the diameter of culture tube bottom, the diameter of through-hole is greater than the diameter of culture tube, the internal diameter of flexible urceolus equals the diameter of through-hole, the vertical face that the slider side face was located is close to the vertical face setting that the culture tube center was located, the diameter of flexible inner tube and the length of puddler and the diameter that is less than the culture tube, puddler and the crisscross even setting of gas vent, the trachea is the hose.
Furthermore, the top end face of the cover plate is flush with the top end face of the incubator, and the centrifugal belt wheel, the centrifugal rotating shaft, the driven belt wheel, the screw rod, the main belt wheel and the output shaft are connected through couplers.
Furthermore, the observation area is just to the region setting that the culture tube lateral wall is located the baffle lower part, control panel is including display screen and control button, control panel control solenoid valve, centrifugal motor and servo motor electrical property work.
Furthermore, the top end and the bottom end of the opening and closing chute are respectively flush with the top end and the bottom end of the opening and closing opening, the height of the opening and closing plate is equal to that of the opening and closing opening, the vertical distance from the bottom end of the magnetic plate to the top end of the opening and closing opening is equal to that of the opening and closing opening, and the top end of the opening and closing plate is in magnetic fit with the magnetic plate.
Furthermore, the horizontal plane of the bottom end of the through groove is higher than the horizontal plane of the top end of the through groove, and the diameter of the semicircular hole is equal to that of the through hole.
Furthermore, the centrifugal motor side is fixed with the connecting block, connecting block and mounting groove bottom threaded connection have a connecting screw, apron and annular threaded connection have a set screw, pole setting lateral wall bottom mounting has the connection pad, connection pad and apron and incubator top threaded connection have a cooperation screw, according to claim a probiotic starter based on biogenic protein, characterized in that: the telescopic outer barrel is characterized in that a connecting arc plate is fixed to the bottom end of the side wall of the telescopic outer barrel, the connecting arc plate and the transverse plate are in threaded connection with positioning screws, a mounting block is fixed to the side wall of the servo motor, the mounting block and the telescopic outer barrel are in threaded connection with mounting screws at the top end of the outer barrel, and the sealing plate and the through groove are in threaded connection with screws.
Advantageous effects
Compared with the known public technology, the technical scheme provided by the invention has the following beneficial effects:
1. the activation culture assembly is arranged, and the culture substrate in the culture tube is used for activating lactobacillus plantarum and adopts an MRS culture medium, and the activation culture assembly comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; 0.29g/L manganese sulfate, 18g/L agar and a stable starter structure, can be ensured for a long time in a proper storage environment, and is simple in preparation method;
2. in the culture process, the servo motor is started to rotate, and the screw rod is driven to rotate under the transmission action of the main belt pulley, the auxiliary belt pulley and the belt, so that the sliding block moves downwards to drive the telescopic inner cylinder to move downwards. The lactobacillus plantarum is led to enter a culture tube, oxygen is conveyed through an air hole by using an air supply device externally connected with an air pipe, and meanwhile, the temperature is controlled by using a heater, so that the activated culture of lactobacillus plantarum is realized;
3. according to the invention, the centrifugal separation component is arranged, after bacterial colony activation culture is completed, the centrifugal motor is started, the culture tube is driven to centrifugally rotate at the speed of 5800rpm of 5000-.
4. The centrifugal motor is convenient to assemble and disassemble by arranging the connecting block, the connecting screw, the fixing screw, the connecting disc, the matching screw, the connecting arc plate, the positioning screw, the mounting block and the mounting screw through the threaded matching of the connecting screw and the connecting block, the cover plate is convenient to assemble and disassemble through the threaded matching of the fixing screw and the ring groove, and the vertical rod is convenient to assemble and disassemble through the matching of the connecting disc and the matching screw; the assembly and disassembly of the telescopic outer barrel are facilitated by the cooperation of the connecting arc plate and the positioning screws, the assembly and disassembly of the servo motor are facilitated by the cooperation of the mounting block and the mounting screws, and the assembly and disassembly of the sealing plate are facilitated by the cooperation of the screws.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.
FIG. 1 is a schematic view of the preparation process of the present invention
FIG. 2 is a schematic view of the main structure of the present invention;
FIG. 3 is a schematic view of a first cross-sectional structure of the present invention;
FIG. 4 is an enlarged view of part A of the present invention;
FIG. 5 is an enlarged view of the portion B of the present invention;
FIG. 6 is an enlarged view of the part C of the present invention;
FIG. 7 is an enlarged view of the D-section of the present invention;
FIG. 8 is a schematic view of the structure of the incubator of the present invention;
FIG. 9 is a schematic diagram of a second cross-sectional structure of the present invention;
FIG. 10 is an enlarged view of section E of the present invention;
FIG. 11 is an enlarged view of the portion F of the present invention;
in the figure: 1. incubator 2, culture area 3, freeze-drying area 300, refrigerator 4, separating groove 5, separating plate 6, rotating bearing 7, culture tube 8, outlet 9, electromagnetic valve 10, heater 11, mounting groove 12, centrifugal motor 13, centrifugal rotating shaft 14, centrifugal belt wheel 15, driving wheel 16, centrifugal belt 17, through groove 18, ring groove 19, cover plate 20, handle 21, vertical rod 22, transverse plate 23, through hole 24, telescopic outer cylinder 25, sliding groove 26, screw 27, sliding block 28, connecting bearing 29, telescopic inner cylinder 30, air hole 31, stirring 32, air tube 33, driven belt wheel 34, servo motor 35, output shaft 36, main belt wheel 37, belt 38, observation area 39, control panel 40, opening and closing port 41, opening and closing sliding groove 42, opening and closing slide block 43, opening and closing plate 44, magnetic plate 45, mounting sliding groove 46, stopper 47, mounting slide block 48, freezing slide block 48, and freeze-drying belt wheel The dry box 49, the through groove 50, the closing plate 51, the auxiliary handle 52, the semicircular hole 53, the connecting block 54, the connecting screw 55, the fixing screw 56, the connecting disc 57, the matching screw 58, the connecting arc plate 59, the positioning screw 60, the mounting block 61 and the mounting screw 62 are arranged in the box.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention. It is to be understood that the embodiments described are only a few embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be further described with reference to the following examples.
Example 1
Referring to fig. 1, a probiotic starter based on a biogenic protein, which comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; manganese sulfate 0.29g/L and agar 18 g/L.
A preparation process of a probiotic starter based on a source protein further comprises the following preparation process:
s1: taking lactobacillus plantarum as a fermentation strain, putting the lactobacillus plantarum into a culture medium base solution used by an activation culture assembly, and introducing oxygen for culturing for 16-28h at the temperature of 26-30 ℃;
s2: carrying out centrifugal separation on the activated and cultured thalli by a centrifugal separation component at the rotating speed of 5000-;
s3: dissolving the separated thallus by using a freeze-drying agent;
s4: freeze-drying at-55 deg.C to-35 deg.C to obtain Lactobacillus plantarum freeze-dried strain.
The invention carries out activated culture on lactobacillus plantarum by arranging an activated culture assembly and utilizing a culture substrate in a culture tube, wherein the substrate adopts an MRS culture medium and comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; 0.29g/L manganese sulfate, 18g/L agar and a stable starter structure, can be ensured for a long time in a proper storage environment, and is simple in preparation method;
example 2
Example 2 is a further modification to example 1:
as shown in figures 2, 3, 4, 7 and 8,
a probiotic starter based on a biogenic protein comprises an incubator 1, wherein a culture area 2 and a freeze-drying area 3 are arranged in the incubator 1, the size of the culture area 2 is the same as that of the freeze-drying area 3, a separation groove 4 is arranged between the culture area 2 and the freeze-drying area 3, the length of the separation groove 4 is larger than that of the culture area 2 in the direction, the width of the separation groove 4 is equal to that of the culture area 2 in the direction, a partition plate 5 is arranged in the separation groove 4, an activation culture assembly is arranged above the partition plate 5, and comprises a culture tube 7, an outlet 8, an electromagnetic valve 9, a heater 10, an upright rod 21, a transverse plate 22, a through hole 23, a telescopic outer tube 24, a sliding groove 25, a screw rod 26, a sliding block 27, a connecting bearing 28, a telescopic inner tube 29, an air hole 30, a stirring rod 31, an air tube 32, a secondary belt pulley 33, a servo motor 34, an output;
cultivate 7 settings of pipe on baffle 5 and the bottom runs through 5 bottoms settings of baffle, cultivate 7 bottoms of pipe and be less than 2 tops in culture area, cultivate 7 inside culture substrate that are provided with of pipe, the culture substrate that uses in the culture pipe 7 is MRS culture medium, and its constitution is: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; 0.29g/L of manganese sulfate, 18g/L of agar, 6.2-6.5 of pH, sterilization is carried out for 30 minutes at 121 ℃ and 0.1Mpa, the thalli cultured in the culture tube 7 is lactobacillus plantarum, the outlet 8 is arranged at the bottom end of the culture tube 7, the diameter of the outlet 8 is smaller than that of the bottom end of the culture tube 7, the electromagnetic valve 9 is arranged in the outlet 8, the heater 10 is symmetrically arranged at the center of the side wall of the culture area 2, the upright posts 21 are symmetrically fixed at two sides of the center of the top end of the incubator 1, the transverse plates 22 are fixed at the top ends of the upright posts 21, the through holes 23 are arranged at the center of the transverse plates 22, the diameter of the through holes 23 is larger than that of the culture tube 7, the telescopic outer cylinders 24 are fixed at the center of the top ends of the transverse plates 22, the inner diameters of the telescopic outer cylinders 24 are equal to the diameter of the through holes 23, the screw 26 penetrates through the sliding block 27 in a threaded manner, the vertical surface of the side surface of the sliding block 27 is close to the vertical surface of the center of the culture tube 7, the connecting bearing 28 is arranged between the two groups of sliding blocks 27, the telescopic inner cylinder 29 is arranged in the connecting bearing 28, the diameter of the telescopic inner cylinder 29 and the length of the stirring rod 31 are smaller than the diameter of the culture tube 7, the telescopic inner cylinder 29 is hollow, air holes 30 and stirring rods 31 are uniformly arranged on the side wall of the telescopic inner cylinder 29, the stirring rods 31 and the air holes 30 are uniformly arranged in a staggered manner, the air pipe 32 is a hose, the air pipe 32 is communicated with the top end of the telescopic inner cylinder 29 and movably penetrates through the top end of the telescopic outer cylinder 24, the air pipe 32 is externally connected with air supply equipment, the driven belt pulley 33 is arranged at the top end of the screw 26, the servo motor 34 is arranged; the lactobacillus plantarum is activated and cultured by utilizing a culture substrate in the culture tube 7, the substrate adopts an MRS culture medium, in the culture process, the servo motor 34 is started, and the screw rod 26 is driven to rotate under the transmission action of the main belt pulley 36, the auxiliary belt pulley 33 and the belt 37, so that the slide block 27 moves downwards to drive the telescopic inner cylinder 29 to move downwards. The lactobacillus plantarum is introduced into the culture tube 7, oxygen is supplied through the air hole 30 by an external air supply device connected with the air tube 32, and the temperature is controlled by the heater 10, so that the activated culture of lactobacillus plantarum is realized.
Example 3
Example 3 is a further modification to example 2:
as shown in fig. 3, fig. 5, fig. 8, fig. 9, fig. 10 and fig. 11, a centrifugal separation assembly is arranged in the incubator 1, and the centrifugal separation assembly comprises a rotary bearing 6, a mounting groove 11, a centrifugal motor 12, a centrifugal rotating shaft 13, a centrifugal belt wheel 14, a driving wheel 15, a centrifugal belt 16, a through groove 17, a ring groove 18, a cover plate 19 and a handle 20; the top end face of the cover plate 19 is flush with the top end face of the incubator 1, the centrifugal belt wheel 14, the centrifugal rotating shaft 13, the secondary belt wheel 33, the screw rod 26, the primary belt wheel 36 and the output shaft 35 are connected through a coupler, the rotating bearing 6 is embedded in the center of the partition plate 5, the bottom end of the incubator 7 penetrates through the bottom end of the partition plate 5 through the rotating bearing 6, the installation groove 11 is formed in the top end of the incubator 1 and on one side of the culture area 2, the centrifugal motor 12 is installed in the installation groove 11, the centrifugal belt wheel 14 is connected to the end portion of the centrifugal rotating shaft 13, the driving wheel 15 is installed at the top end of the side wall of the incubator 7, the through groove 17 is formed in the side wall of the installation groove 11, the centrifugal belt 16 penetrates through the through groove 17 to be driven between the driving wheel 15 and the centrifugal;
an observation area 38 and a control panel 39 are arranged on the side wall of the incubator 1, the observation area 38 is arranged right opposite to the area, located at the lower part of the partition plate 5, of the side wall of the culture tube 7, the control panel 39 comprises a display screen and a control button, the control panel 39 controls the electromagnetic valve 9, the centrifugal motor 12 and the servo motor 34 to electrically work, a refrigerator 300 is arranged on the inner wall of the freeze-drying area 3, an opening and closing opening 40 is arranged on the outer wall of the freeze-drying area 3, opening and closing chutes 41 are symmetrically arranged on two sides of the opening and closing opening 40, an opening and closing slider 42 slides in the opening and closing chute 41, an opening and closing plate 43 is fixed on the side surface of the opening and closing slider 42, a magnetic plate 44 is fixed on the side wall of the incubator 1, an installation chute 45 is arranged at the bottom end of the freeze-drying area 3, a stop 46; a through groove 49 is formed in the side wall of the culture area 2, a closing plate 50 is arranged in the through groove 49, an auxiliary handle 51 is arranged at the center of the side surface of the closing plate 50, and a semicircular hole 52 is formed in the other side surface of the closing plate 50; after the activation culture of the bacterial colony is completed, the centrifugal motor 11 is started, under the action of the centrifugal belt wheel 14, the driving wheel 15 and the centrifugal belt 16, the culture tube 7 is driven to centrifugally rotate at the speed of 5800rpm of 5000-.
Example 4
Example 4 is a further modification to example 3:
as shown in fig. 5, 6, 7, 8 and 11, the top end and the bottom end of the opening and closing chute 41 are respectively flush with the top end and the bottom end of the opening and closing opening 40, the height of the opening and closing plate 43 is equal to the height of the opening and closing opening 40, the vertical distance from the bottom end of the magnetic plate 44 to the top end of the opening and closing opening 40 is equal to the height of the opening and closing opening 40, and the top end of the opening and closing plate 43 is in magnetic fit with the magnetic plate 44; the horizontal plane of the bottom end of the through groove 49 is higher than the horizontal plane of the top end of the through groove 17, and the diameter of the semicircular hole 52 is equal to that of the through hole 23; a connecting block 53 is fixed on the side surface of the centrifugal motor 12, the connecting block 53 and the bottom end of the mounting groove 11 are in threaded connection with a connecting screw 54, and the cover plate 19 and the ring groove 18 are in threaded connection with a fixing screw 55; a connecting disc 56 is fixed at the bottom end of the side wall of the upright rod 21, the connecting disc 56, the cover plate 19 and the top end of the incubator 1 are in threaded connection with a matching screw 57, a connecting arc plate 58 is fixed at the bottom end of the side wall of the telescopic outer cylinder 24, the connecting arc plate 58 and the transverse plate 5 are in threaded connection with a positioning screw 59, a mounting block 60 is fixed at the side wall of the servo motor 34, a mounting screw 61 is in threaded connection with the top end of the telescopic outer cylinder 24, and a screw 62 is in threaded connection with the closing plate 50 and; the assembly and disassembly of the centrifugal motor 11 are facilitated by the threaded fit of the connecting screw 54 and the connecting block 53, the assembly and disassembly of the cover plate 19 are facilitated by the threaded fit of the fixing screw 55 and the annular groove 18, and the assembly and disassembly of the upright rod 21 are facilitated by the fit of the connecting disc 56 and the matching screw 57; the assembly and disassembly of the telescopic outer cylinder 24 are facilitated by the cooperation of the connecting arc plate 58 and the positioning screw 59, the assembly and disassembly of the servo motor 34 are facilitated by the cooperation of the mounting block 60 and the mounting screw 61, and the assembly and disassembly of the closing plate 50 are facilitated by the cooperation of the screw 62
When in use:
s1: taking lactobacillus plantarum as a fermentation strain, putting the lactobacillus plantarum into a culture medium base solution used by an activation culture assembly, and introducing oxygen for culturing for 16-28h at the temperature of 26-30 ℃; the culture substrate used in the culture tube 7 is an MRS culture medium, and comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; 0.29g/L manganese sulfate and 18g/L agar, starting a servo motor 34, and driving the screw rod 26 to rotate under the transmission action of the main belt pulley 36, the auxiliary belt pulley 33 and the belt 37, so that the slide block 27 moves downwards and drives the telescopic inner cylinder 29 to move downwards. The lactobacillus plantarum is fed into the culture tube 7, oxygen is conveyed through the air hole 30 by using an air supply device externally connected with an air tube 32, and the temperature is controlled by using the heater 10, so that the activated culture of lactobacillus plantarum is realized;
s2: carrying out centrifugal separation on the activated and cultured thalli by a centrifugal separation component at the rotating speed of 5000-; after the activation culture of the bacterial colony is completed, starting a centrifugal motor 11, and driving the culture tube 7 to centrifugally rotate at 5000-;
s3: dissolving the separated thallus by using a freeze-drying agent; after the separation is finished, the electromagnetic valve 9 is opened, the observation area 38 is used for observation, the opening and closing of the electromagnetic valve 9 are controlled, the bacterial colony is released, the bacterial colony falls into the freeze-drying box 48 in the freeze-drying area 3 for subsequent treatment, and the device integrates an activation culture assembly and a centrifugal separation assembly of the bacterial colony together, so that the uniform operation and control of preparation personnel are facilitated;
s4: freeze-drying at-55 deg.C to-35 deg.C to obtain Lactobacillus plantarum lyophilized bacteria; utilize refrigerator 300 to carry out temperature control, freeze-dry into fungus powder to the bacterial colony in the freeze-drying box 48, realize the preparation of probiotic starter, after the preparation is accomplished, utilize the cooperation of opening and close spout 41 and opening and close slider 42, will open and close board 43 and open, under the cooperation of installation spout 45 and installation slider 47, realize taking out of freeze-drying box 48.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. A probiotic starter based on a protein of origin, characterized in that: the probiotic leavening agent comprises the following components: peptone 10 g/L; 5g/L of yeast extract; 2g/L of diammonium citrate; 20g/L of glucose; 10g/L of beef extract; tween-801 g/L; 2g/L of dipotassium phosphate; magnesium sulfate 0.58 g/L; manganese sulfate 0.29g/L and agar 18 g/L.
2. The process for preparing a prebiotic protein based probiotic starter according to claim 1, wherein: also comprises the following preparation process:
s1: taking lactobacillus plantarum as a fermentation strain, putting the lactobacillus plantarum into a culture medium base solution used by an activation culture assembly, and introducing oxygen for culturing for 16-28h at the temperature of 26-30 ℃;
s2: carrying out centrifugal separation on the activated and cultured thalli by a centrifugal separation component at the rotating speed of 5000-;
s3: dissolving the separated thallus by using a freeze-drying agent;
s4: freeze-drying at-55 deg.C to-35 deg.C to obtain Lactobacillus plantarum freeze-dried strain.
3. Preparation device for preparing a probiotic starter based on a protein of origin according to claim 1, comprising an incubator (1), characterized in that: the device is characterized in that a culture area (2) and a freeze-drying area (3) are arranged in the incubator (1), a separation groove (4) is arranged between the culture area (2) and the freeze-drying area (3), a partition plate (5) is arranged in the separation groove (4), an activation culture assembly is arranged above the partition plate (5), and comprises a culture tube (7), an outlet (8), an electromagnetic valve (9), a heater (10), a vertical rod (21), a transverse plate (22), a through hole (23), a telescopic outer cylinder (24), a sliding groove (25), a screw rod (26), a sliding block (27), a connecting bearing (28), a telescopic inner cylinder (29), an air hole (30), a stirring rod (31), an air pipe (32), a secondary belt pulley (33), a servo motor (34), an output shaft (35), a main belt pulley (36) and a belt (37);
the culture tube (7) is arranged on the partition plate (5) and the bottom end of the culture tube penetrates through the bottom end of the partition plate (5), a culture substrate is arranged in the culture tube (7), the bottom end of the culture tube (7) is arranged at the outlet (8), the electromagnetic valve (9) is arranged in the outlet (8), the heater (10) is symmetrically arranged at the center of the side wall of the culture area (2), the upright rods (21) are symmetrically fixed at the two sides of the center of the top end of the culture box (1), the transverse plate (22) is fixed at the top end of the upright rods (21), the through hole (23) is arranged at the center of the transverse plate (22), the telescopic outer barrel (24) is fixed at the center of the top end of the transverse plate (22), the sliding grooves (25) are symmetrically arranged on the inner wall of the telescopic outer barrel (24), the screw rod (26) is rotated to penetrate through the telescopic outer barrel (24) at the top end and the bottom end, the screw rod (26) penetrates through the sliding blocks (27) in a threaded mode, the connecting bearings (28) are arranged between the side faces of the two groups of sliding blocks (27), the telescopic inner cylinder (29) is installed in the connecting bearings (28), the telescopic inner cylinder (29) is hollow, air holes (30) and a stirring rod (31) are uniformly formed in the side wall of the telescopic inner cylinder (29), the air pipe (32) is communicated with the top end of the telescopic inner cylinder (29) and movably penetrates through the top end of the telescopic outer cylinder (24), the air pipe (32) is externally connected with air supply equipment, the driven belt pulley (33) is arranged at the top end of the screw rod (26), the servo motor (34) is installed at the top end of the telescopic outer cylinder (24), the main belt pulley (36) is connected with the output shaft (35), and the belt (37) is arranged between the two groups of driven belt;
a centrifugal separation component is arranged in the incubator (1), and comprises a rotating bearing (6), a mounting groove (11), a centrifugal motor (12), a centrifugal rotating shaft (13), a centrifugal belt wheel (14), a driving wheel (15), a centrifugal belt (16), a through groove (17), an annular groove (18), a cover plate (19) and a handle (20);
the rotating bearing (6) is embedded in the center of the clapboard (5), the bottom end of the culture tube (7) penetrates through the bottom end of the clapboard (5) through the rotating bearing (6), the mounting groove (11) is arranged at one side of the top end of the incubator (1) in the culture area (2), the centrifugal motor (12) is arranged in the mounting groove (11), the centrifugal belt wheel (14) is connected with the end part of the centrifugal rotating shaft (13), the driving wheel (15) is arranged at the top end of the side wall of the culture tube (7), the through groove (17) is arranged on the side wall of the mounting groove (11), the centrifugal belt (16) passes through the through groove (17) and is transmitted between the driving wheel (15) and the centrifugal belt wheel (14), the ring groove (18) is arranged at the top end edge of the mounting groove (11), the cover plate (19) is mounted in the ring groove (18), the handles (20) are symmetrically arranged at two sides of the center of the top end of the cover plate (19);
the side wall of the incubator (1) is provided with an observation area (38) and a control panel (39), a refrigerator (300) is arranged on the inner wall of the freeze-drying area (3), an opening and closing opening (40) is arranged on the outer wall of the freeze-drying area (3), opening and closing chutes (41) are symmetrically arranged at two sides of the opening and closing port (40), an opening and closing slide block (42) slides in the opening and closing chutes (41), an opening and closing plate (43) is fixed on the side surface of the opening and closing slide block (42), a magnetic plate (44) is fixed on the side wall of the incubator (1), the bottom end of the freeze-drying area (3) is provided with a mounting sliding chute (45), the center of the bottom end of the opening and closing plate (43) is matched with a stop block (46), a mounting sliding block (47) is arranged in the mounting sliding groove (45) in a sliding manner, a freeze-drying box (48) is mounted at the top end of the mounting sliding block (47), and freeze-drying agent is arranged in the freeze-drying box (48);
the culture area (2) side wall is provided with a through groove (49), a closing plate (50) is arranged inside the through groove (49), an auxiliary handle (51) is arranged at the center of the side face of the closing plate (50), and a semicircular hole (52) is formed in the other side face of the closing plate (50).
4. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: the culture area (2) and the freeze-drying area (3) are the same in size, the length of the separation groove (4) is larger than the length of the culture area (2) in the direction, and the width of the separation groove (4) is equal to the length of the culture area (2) in the direction.
5. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: cultivate pipe (7) bottom and be less than cultivation district (2) top, the diameter of export (8) is less than the diameter of cultivation pipe (7) bottom, the diameter of through-hole (23) is greater than the diameter of cultivation pipe (7), the inner diameter of flexible urceolus (24) equals the diameter of through-hole (23), the vertical face that slider (27) side located is close to the vertical face setting that cultivation pipe (7) center located, the length of the diameter of flexible inner tube (29) and puddler (31) and the diameter that is less than cultivation pipe (7), puddler (31) and gas pocket (30) crisscross even setting, trachea (32) are the hose.
6. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: the top end face of the cover plate (19) is flush with the top end face of the incubator (1), and the centrifugal belt wheel (14), the centrifugal rotating shaft (13), the driven belt wheel (33), the screw rod (26), the main belt wheel (36) and the output shaft (35) are connected through couplers.
7. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: the observation area (38) is just to the regional setting that the lateral wall of culture tube (7) is located baffle (5) lower part, control panel (39) are including display screen and control button, control panel (39) control solenoid valve (9), centrifugal motor (12) and servo motor (34) electrical property work.
8. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: the top end and the bottom end of the opening and closing chute (41) are respectively flush with the top end and the bottom end of the opening and closing opening (40), the height of the opening and closing plate (43) is equal to the height of the opening and closing opening (40), the vertical distance from the bottom end of the magnetic plate (44) to the top end of the opening and closing opening (40) is equal to the height of the opening and closing opening (40), and the top end of the opening and closing plate (43) is in magnetic fit with the magnetic plate (44).
9. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: the horizontal plane of the bottom end of the through groove (49) is higher than the horizontal plane of the top end of the through groove (17), and the diameter of the semicircular hole (52) is equal to that of the through hole (23).
10. The apparatus for preparing a prebiotic protein based probiotic starter culture according to claim 3, wherein: centrifugal motor (12) side is fixed with connecting block (53), connecting block (53) and mounting groove (11) bottom threaded connection have connecting screw (54), apron (19) and annular (18) threaded connection have set screw (55), pole setting (21) lateral wall bottom mounting has connection pad (56), connection pad (56) and apron (19) and incubator (1) top threaded connection have cooperation screw (57), according to claim 1 a probiotic starter based on thing source protein, its characterized in that: flexible urceolus (24) lateral wall bottom mounting has connection arc board (58), connect arc board (58) and diaphragm (5) threaded connection has set screw (59), servo motor (34) lateral wall is fixed with installation piece (60), installation piece (60) and flexible be urceolus (24) top threaded connection have mounting screw (61), closing plate (50) and wear groove (49) threaded connection have screw (62).
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