CN112602706A - Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof - Google Patents
Bactrocera dorsalis female insect trapping and killing composition and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N51/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds having the sequences of atoms O—N—S, X—O—S, N—N—S, O—N—N or O-halogen, regardless of the number of bonds each atom has and with no atom of these sequences forming part of a heterocyclic ring
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Abstract
The invention belongs to the technical field of prevention and control of agricultural and forestry pests, and particularly relates to a female citrus fruit fly trapping and killing composition and a preparation method thereof. The trapping and killing composition consists of transparent soap base, Pichia kluyveri, sugar, colorant, pesticide and attractant. The trapping and killing composition has excellent prevention and control effects on fruit flies, particularly female flies, has long lasting period, greatly reduces the using amount of pesticides, has cheap raw materials, simple preparation method, is environment-friendly and has good application prospect.
Description
Technical Field
The invention belongs to the technical field of prevention and control of agricultural and forestry pests, and particularly relates to a female citrus fruit fly trapping and killing composition and a preparation method thereof.
Background
Fruit fly of orangeBactrocera dorsalisAlso called Oriental fruit fly, fruit maggot, etc., belonging to the Diptera family of Bactroceraceae family of TephritidaeBactroceraIs a destructive pest. The insect has strong invasion and diffusion capability and environmental adaptability, can be widely distributed in southeast Asia and Pacific region, and can harm more than 250 kinds of vegetables and fruits including mango, orange, guava, banana, orange, pepper, tomato, etc. In the control work of Bactrocera dorsalis Hendel, how to reduce the number of female insects is the key point for controlling the population. Therefore, foreign prevention and control of Bactrocera dorsalis are mainly realized by hanging food-source bait with strong attraction to female Bactrocera dorsalis in a large scale or spraying the food-source bait and pesticide after mixing. However, conventional food source baits such as GF-120, Ceretrap, Nulure, torula baits, etc. are not sold in China. Therefore, the female citrus fruit fly is single in prevention and control selection in China, and the species group and harm of the female citrus fruit fly are mainly controlled and reduced by spraying a large amount of chemical agents frequently. However, excessive use of chemical pesticides for controlling bactrocera dorsalis can cause serious damage and threats to the natural ecological environment, food safety and the like. Therefore, by combining the chemical ecology of insects and the behavior of insects, the degradable trapping and killing composition which has olfactory stimulation, gustatory stimulation, visual stimulation and oviposition stimulation to female bactrocera dorsalis is developed, and the degradable trapping and killing composition is very significant for promoting the development of green and sustainable prevention and control technology of the bactrocera dorsalis.
Through a large number of experiments, the soap base is unexpectedly found to be the best carrier of the Bactrocera dorsalis attractant and the insecticidal active substance, the surface layer can be continuously updated due to the characteristic of water solubility, the internal attracting activity and the insecticidal active ingredient are exposed, and the soap base is the best long-acting carrier under the field condition. In addition, volatile components of bacterial microorganisms having a strong attraction effect on female bactrocera dorsalis are analyzed and screened through GC-MS, 3-methyl-1-butanol, 2-methylpyrazine and trimethylamine are found to be attracting active substances, the three substances are combined with 1-methylpyrrolidine to serve as olfactory stimulation, and then taste stimulation, visual stimulation and spawning stimulation are combined to form the degradable trapping and killing composition having an attraction effect on female bactrocera dorsalis.
Disclosure of Invention
The invention aims to provide a female bactrocera dorsalis trapping and killing composition and a preparation method thereof, which have excellent prevention and control effects on bactrocera dorsalis, particularly female bactrocera dorsalis, have long lasting period, greatly reduce the use amount of pesticides, are environment-friendly and can be naturally degraded.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
the female bactrocera dorsalis trapping and killing composition comprises the following raw materials: transparent soap base, pichia kluyveri, sugar, colorant, pesticide and attractant.
Particularly, the mass ratio of the transparent soap base, the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant is 30-70:1-10:30-50:1-3:1-3: 2-5.
Preferably, the mass ratio of the transparent soap base, the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant is 45-55:5-8:30-40:1-1.5:1-1.5: 2-3.
Preferably, the mass ratio of the transparent soap base, the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant is 51:8:36:1:1: 3.
In particular, the shape of the female citrus fruit fly trapping and killing composition can be controlled by a mould, and the female citrus fruit fly trapping and killing composition comprises cubes, spheres and the like, preferably spheres, and is white, yellow, green, orange, red, preferably yellow.
In particular, the spheres have a diameter of 4 to 8cm, preferably 6 cm.
Further, the attractant consists of 3-methyl-1-butanol, 2-methylpyrazine, trimethylamine and 1-methylpyrrolidine, and the mass ratio of the attractant to the 1-methylpyrazine is 0.8:0.1:0.05: 0.05.
The pesticide is one or more of abamectin, spinetoram, spinosad, thiamethoxam, imidacloprid or acetamiprid.
The field lasting period of the female bactrocera dorsalis trapping and killing composition is at least maintained for two months.
The application of the female citrus fruit fly trapping and killing composition in controlling the female citrus fruit flies is to hang 50 female citrus fruit fly trapping and killing compositions on fruit trees 1.5 meters away from the ground when in use.
The preparation method of the composition for trapping and killing the female bactrocera dorsalis comprises the following steps of:
(1) mixing beef tallow, coconut oil, sodium hydroxide, water and polyethylene glycol according to the mass ratio of 30:30:9:21:10, fully reacting for 30min at 70 ℃ and 2000rpm, adding saturated sodium chloride solution, and standing and layering for 30min at 70 ℃. The clear soap base was collected through a 100 mesh sieve.
(2) The transparent soap base was placed in a forced air drying oven and dried at 30 ℃ for 24 hours.
(3) Preparing materials according to the proportion of the raw materials contained in each 100g of the trapping and killing composition, and weighing the transparent soap base, the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant according to the proportion.
(4) And (3) stirring the transparent soap base obtained in the step (2) at 70 ℃ until the transparent soap base is melted, and then sequentially adding the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant, and uniformly stirring until no granular thick slurry exists, thus obtaining the trapping and killing composition.
(5) Placing the trapping and killing composition in a latex mold, wherein a spherical latex mold is provided with small holes, penetrating an iron wire into the mold filled with the poison bait concentrated slurry through the small holes, and then rapidly freezing the mold at the temperature of minus 80 ℃ for 20 minutes to obtain the formed bactrocera dorsalis trapping and killing agent.
The invention has the following advantages:
1. the female citrus fruit fly trapping and killing composition provided by the invention is easy to obtain raw materials, low in price, environment-friendly, pollution-free and capable of being naturally degraded under field conditions, and the duration of the female citrus fruit fly trapping and killing composition is at least maintained for two months under field conditions.
2. The female citrus fruit fly trapping and killing composition can be used for preventing and controlling the female citrus fruit flies, particularly female flies, has an obvious effect, and can effectively control the harm of the field female citrus fruit flies.
Drawings
FIG. 1: the weight degradation tendency of the trapping and killing balls with different matrixes under field conditions;
FIG. 2: a dynamic diagram of female citrus fruit fly population;
FIG. 3: average number of female Bactrocera dorsalis per week;
FIG. 4: fruit damage rate is plotted.
Detailed Description
The advantages and features of the present invention will become more apparent from the following detailed description of specific embodiments thereof. These examples are illustrative only and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications may be made without departing from the spirit and scope of the invention.
The female bactrocera dorsalis trapping and killing composition comprises the following raw materials: transparent soap base, pichia kluyveri, sugar, colorant, pesticide and attractant.
Further, the attractant consists of 3-methyl-1-butanol, 2-methylpyrazine, trimethylamine and 1-methylpyrrolidine, and the mass ratio of the attractant to the 1-methylpyrazine is 0.8:0.1:0.05: 0.05.
The pesticide is one or more of abamectin, spinetoram, spinosad, thiamethoxam, imidacloprid or acetamiprid.
The preparation method of the composition for trapping and killing the female bactrocera dorsalis comprises the following steps of:
(1) mixing beef tallow, coconut oil, sodium hydroxide, water and polyethylene glycol according to the mass ratio of 30:30:9:21:10, fully reacting for 30min at 70 ℃ and 2000rpm, adding saturated sodium chloride solution, and standing and layering for 30min at 70 ℃. The clear soap base was collected through a 100 mesh sieve.
(2) The transparent soap base is placed in a forced air drying oven and dried for 24 hours at the temperature of 30 ℃.
(3) Preparing materials according to the weight ratio of the raw materials per 100g of the trapping and killing composition, namely transparent soap base, pichia kluyveri, sugar, a colorant, an insecticide and an attractant, wherein the weight ratio of the attractant is 51:5:3:36:1:1: 3.
(4) The transparent soap base is stirred to be molten at 70 ℃, and then the pichia kluyveri, the colorant, the insecticide and the attractant are sequentially added and stirred uniformly until no granular thick slurry exists.
(5) 160g of the trapping and killing composition is placed in a spherical latex mold with the diameter of 6cm, small holes are arranged on the spherical latex mold, an iron wire with the length of 30cm penetrates into the spherical latex mold filled with the trapping and killing thick slurry through the small holes, and then the spherical latex mold is placed at the temperature of minus 80 ℃ for quick freezing for 20 minutes to obtain the trapping and killing ball.
Example 1
Respectively suspending the following treatments in field conditions, 1. poison bait using transparent soap base as matrix; 2. poison bait using paraffin as matrix. The differently treated baits were taken back to the laboratory each week, their weights were weighed, the experiment time ranged from 7 months 4 days to 9 months 12 days, and the experiment was continued for ten weeks.
The two treated bait balls were suspended in a bioassay small cage containing 50 female citrus fruit flies, and the number of visits the female citrus fruit flies had to different treatments was recorded every hour for 10 consecutive hours. After 24 hours, the mortality rate was counted. Fresh trap balls were used as controls. Each treatment was 3 replicates.
As shown in figure 1, after 2 weeks of field use, the weight of the killing ball based on the transparent soap base prepared by the invention is significantly lower than that of the killing ball based on paraffin (P > 0.05). After ten weeks of field use, the weight of the trapping ball taking the transparent soap base as the matrix is reduced from 160.43 +/-0.81 g to 91.11 +/-3.61 g, the reduction amplitude reaches 69.32 +/-2.88 g, while the weight of the trapping ball taking the paraffin as the matrix is reduced from 160.33 +/-0.44 g to 158.97 +/-0.54 g, and the reduction amplitude is only 1.36g +/-0.12 g, thus proving that the trapping ball taking the transparent soap base as the matrix has the self-degradation capability.
TABLE 1 indoor cage test for trap-kill ball duration (mean. + -.% standard error)
The results of the indoor small cages show that (table 1), the killing rate of the trapping balls taking the transparent soap base as the matrix to the female citrus fruit flies in ten weeks of field suspension is not significantly different from that of the fresh trapping balls (P < 0.01), and the killing effect of the trapping balls to the female citrus fruit flies in the sixth week, the seventh week, the eighth week, the ninth week and the tenth week is significantly higher than that of the trapping balls in the treatment 2 (P < 0.01). Furthermore, the number of visits by bactrocera dorsalis females was significantly higher for treatment 1 than for treatment 2 after eight weeks of field suspension (P < 0.05).
Example 2
The experimental method comprises the following steps: two navel orange trees were placed in a raw measuring cage (3 m.times.3 m.times.1.8 m) with no fruit on the navel orange trees. The following treatments, treatment 1, were hung on each of the two trees: treating with luring ball without adding active pesticide; 2: and (5) ripening the navel orange. 200 sexual-mature but non-oviposition female bactrocera dorsalis are grabbed and put into a large cage. The number of bactrocera dorsalis females that lay eggs on the respective treatments was counted every hour. The experiment was carried out for a time period of 9:00 to 18: 00. Navel orange tree positions were transposed every 2 hours. The experiment was repeated 6 times.
Table 2: the number of female citrus fruit flies laying eggs on the attracting ball and mature navel orange (mean value. + -. standard error).
As shown in the results in Table 2, there was no significant difference in the number of Bactrocera dorsalis females that lay eggs on the attractant ball and mature navel orange.
Example 3
Since there were no control of the same category, the control was a standard control bait for fruit flies (torula bait) in the field attraction experiment. In the present invention, round yeast bait was purchased from ISCA Technologies, USA.
The inducing method comprises the following steps: treatment 1: 6 pieces of round yeast were dissolved in 1200ml of water, and each trap was filled with 200ml of round yeast solution, which served as a control. And (3) treatment 2: the trapping and killing ball is placed in a self-made transparent trapper. The experiment is carried out in an ecological garden lemon garden in Longtai mountain of Fuhou county of Fuzhou city in Fujian province, 3 areas are selected in the garden, and each area is separated by 20 meters. 2 lemon trees selected in each area are respectively suspended in the treatment group and the comparison group, the suspension height is 1.5m, and the distance between the two points is 10 m. The test time was from 27 days 9/10/25 days 2020, the number of fruit flies trapped in the trap was investigated every week, and the positions of the traps in the same block were changed clockwise every week to eliminate positional deviation.
Table 3: the ball and round yeast bait is used for killing female citrus fruit fly attracted every week averagely (mean value plus or minus standard error).
As shown in the results in Table 3, the total number of Bactrocera dorsalis Hendel, the number of female insects and the proportion of female insects trapped by the trapping ball in the lemon garden have no significant difference from the standard control bait (Torulopsis aurantiaca bait) for Bactrocera dorsalis Hendel.
Example 4
Since there is no control of the same category, yellow plate was used as control in the field fruit fly visit frequency experiment
The test method comprises the following steps: the experiment is carried out in an ecological garden of a mountain of Longtaishan mountain in Fuzhou city of Fujian province, a single trapping and killing ball is hung at 1.5m of a navel orange tree, and a yellow plate is hung on the navel orange tree at an interval of 5 m. The number of fly visits to the trapping composition within one hour was observed and the number of traps within one hour of yellow plates was used as a control. After one hour observation, the trapping and killing composition was removed and hung on navel orange trees spaced 10m apart, while a yellow plate was hung on navel orange trees spaced 5m apart, and a new round of testing was started. The test was conducted at 10:00-15:00 on 25, 1, and 8 months of 11, 2020, and was observed for a total of three persons, and was repeated 45 times cumulatively.
Table 4: average visit times of female bactrocera dorsalis to trap ball in navel orange garden (average value +/-standard error)
As shown in the results in table 4, the number of visits of female citrus fruit flies to the trapping and killing ball in the navel orange garden was significantly higher than that of the yellow plate in different observation dates.
Example 5
The test method comprises the following steps: the test was conducted in the Jiang Yang farm sweet persimmon orchard in Fuzhou city, Fujian county, Fujian province, and 6 areas were selected, each area having a height of 0.5 hectare. Wherein three areas are treated by suspending trapping and killing balls, the suspension height of the trapping and killing balls is 1.5m, and the density is 50 balls per hectare. The remaining three areas were subjected to spinosyn treatment. A trap is respectively arranged at the center of each of the 6 areas, and round yeast bait is filled in the trap and is used for monitoring the number of female bactrocera dorsalis. The fruit damage rates in the different treatment areas were investigated at 11/15/11/22/2020. And respectively selecting 10 sweet persimmon trees in each area, respectively observing 5 sweet persimmon trees on branches of east, west, south and north of each sweet persimmon tree, and marking as harm if egg laying holes exist. 200 sweet persimmons were investigated per area. The experimental time ranged from 9 months 20 days to 11 months 22 days in 2020.
The results in FIGS. 2, 3 and 4 show that there was no significant difference in the average number of female Bactrocera dorsalis traps and the fruit damage rate in the area receiving the ball trap treatment and the spinosad treatment.
Claims (10)
1. The female bactrocera dorsalis trapping and killing composition is characterized by comprising the following components in parts by weight: the method comprises the following raw materials: transparent soap base, pichia kluyveri, sugar, colorant, pesticide and attractant.
2. The composition for trapping and killing female bactrocera dorsalis according to claim 1, wherein the composition comprises the following components in percentage by weight: transparent soap base, pichia kluyveri, sugar, colorant, pesticide and attractant in a mass ratio of 30-70:1-10:30-50:1-3:1-3: 2-5.
3. The composition for trapping and killing female bactrocera dorsalis according to claim 1, wherein the composition comprises the following components in percentage by weight: the attractant consists of 3-methyl-1-butanol, 2-methylpyrazine, trimethylamine and 1-methylpyrrolidine in a mass ratio of 0.8:0.1:0.05: 0.05.
4. The composition for trapping and killing female bactrocera dorsalis according to claim 1, wherein the composition comprises the following components in percentage by weight: the pesticide is one or more of abamectin, spinetoram, spinosad, thiamethoxam, imidacloprid or acetamiprid.
5. The method for preparing the composition for trapping and killing Bactrocera dorsalis according to any one of claims 1-4, wherein: comprises the following steps in sequence:
(1) mixing butter, coconut oil, sodium hydroxide, water and polyethylene glycol in proportion, heating, stirring for reaction, adding saturated sodium chloride solution, standing at 70 deg.C, and layering for 30 min; collecting transparent soap base through 100 mesh filter screen;
(2) placing the transparent soap base in a forced air drying oven, and drying;
(3) preparing materials according to the proportion of raw materials contained in each 100g of the trapping and killing composition, and weighing the transparent soap base, the pichia kluyveri, the sugar, the colorant, the pesticide and the attractant according to the proportion;
(4) stirring the transparent soap base obtained in the step (2) at 70 ℃ until the transparent soap base is melted, then sequentially adding the pichia kluyveri, sugar, a colorant, an insecticide and an attractant, and uniformly stirring until no granular thick slurry exists, thus obtaining the trapping and killing composition;
(5) and (3) placing the trapping and killing composition in a latex mold, wherein a small hole is formed in the spherical latex mold, and after an iron wire penetrates into the mold filled with the poison bait concentrated slurry through the small hole, placing the mold into a quick freezing mode to obtain the formed bactrocera dorsalis trapping and killing agent.
6. The method of claim 5, wherein: in the step (1), the mass ratio of the beef tallow to the coconut oil to the sodium hydroxide to the water to the polyethylene glycol is 30:30:9:21: 10.
7. The method of claim 5, wherein: the heating and stirring reaction in the step (1) is specifically a full reaction for 30min at 70 ℃ and 2000 rpm.
8. The method of claim 5, wherein: the drying in the step (2) is specifically as follows: drying at 30 ℃ for 24 hours.
9. The method of claim 5, wherein: the quick freezing in the step (5) is specifically quick freezing for 20 minutes at the temperature of minus 80 ℃.
10. Use of the bactrocera dorsalis trapping and killing composition as claimed in any one of claims 1-4 to control bactrocera dorsalis.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116218917A (en) * | 2022-12-26 | 2023-06-06 | 中国农业科学院深圳农业基因组研究所 | Gene regulation and control method for breeding bactrocera dorsalis |
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| CN116218917A (en) * | 2022-12-26 | 2023-06-06 | 中国农业科学院深圳农业基因组研究所 | Gene regulation and control method for breeding bactrocera dorsalis |
| CN116218917B (en) * | 2022-12-26 | 2024-04-05 | 中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心) | Gene regulation and control method for breeding bactrocera dorsalis |
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