CN112592399B - Preparation method and device for separating bovine serum albumin from bovine serum - Google Patents
Preparation method and device for separating bovine serum albumin from bovine serum Download PDFInfo
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- CN112592399B CN112592399B CN202011479957.0A CN202011479957A CN112592399B CN 112592399 B CN112592399 B CN 112592399B CN 202011479957 A CN202011479957 A CN 202011479957A CN 112592399 B CN112592399 B CN 112592399B
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- 108091003079 Bovine Serum Albumin Proteins 0.000 title claims abstract description 58
- 229940098773 bovine serum albumin Drugs 0.000 title claims abstract description 58
- 239000012888 bovine serum Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000000502 dialysis Methods 0.000 claims abstract description 54
- 238000000926 separation method Methods 0.000 claims abstract description 51
- 238000006243 chemical reaction Methods 0.000 claims abstract description 36
- 238000004519 manufacturing process Methods 0.000 claims abstract description 16
- 238000004108 freeze drying Methods 0.000 claims abstract description 15
- 239000006228 supernatant Substances 0.000 claims abstract description 14
- 241000283690 Bos taurus Species 0.000 claims abstract description 12
- 210000004369 blood Anatomy 0.000 claims abstract description 12
- 239000008280 blood Substances 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 10
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 238000005349 anion exchange Methods 0.000 claims abstract description 5
- 238000003756 stirring Methods 0.000 claims description 18
- 239000003814 drug Substances 0.000 claims description 17
- 238000007789 sealing Methods 0.000 claims description 10
- 238000003825 pressing Methods 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims 1
- 239000012716 precipitator Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 description 11
- 238000010586 diagram Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000007781 pre-processing Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- External Artificial Organs (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention belongs to the technical field of bovine serum albumin manufacturing equipment, and particularly discloses a preparation method for separating bovine serum albumin from bovine serum, which comprises the following steps: taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in a bovine serum albumin preparation integrated machine, pressurizing to crush red blood cells in the bovine blood, and performing centrifugal separation to obtain supernatant; introducing the obtained supernatant into a reaction chamber in a bovine serum albumin preparation integrated machine, adding a precipitant, and separating to obtain bovine serum; introducing bovine serum into a vibration dialysis separation chamber of a bovine serum albumin preparation integrated machine, adding PBS solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution; introducing the solution into an exchange chamber in a bovine serum albumin preparation integrated machine to obtain refined bovine serum albumin by an anion exchange method; the refined bovine serum albumin is introduced into a freeze-drying chamber in a bovine serum albumin manufacturing integrated machine, so as to obtain bovine serum albumin powder.
Description
Technical Field
The invention relates to the related field of bovine serum albumin manufacturing equipment, in particular to a preparation method and a device for separating bovine serum albumin from bovine serum.
Background
The existing bovine serum albumin is mostly prepared by separating red blood cells from blood plasma and then extracting the red blood cells, but the red blood cells also contain protein, so that materials are wasted, meanwhile, the existing bovine serum albumin is prepared by more steps and is usually distributed into different equipment for preparation and production, so that workers are required to continuously transfer the materials, the processing progress and the preparation quality of the bovine serum albumin are delayed, and a preparation method and a device for separating the bovine serum albumin from the bovine serum are designed to solve the problems.
Disclosure of Invention
The invention aims to provide a preparation method and a device for separating bovine serum albumin from bovine serum, which are used for solving the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for separating bovine serum albumin from bovine serum, comprising the steps of:
s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in a bovine serum albumin preparation integrated machine, pressurizing to crush red blood cells in the bovine blood, and performing centrifugal separation to obtain supernatant;
s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin manufacturing integrated machine, adding a precipitant, and separating to obtain bovine serum;
s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber of a bovine serum albumin manufacturing integrated machine, adding PBS solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution;
s4, introducing the solution in the step S3 into an exchange chamber in a bovine serum albumin preparation integrated machine, and obtaining the refined and pure bovine serum albumin by an anion exchange method;
and S5, introducing the refined bovine serum albumin obtained in the step S4 into a freeze-drying chamber in a bovine serum albumin manufacturing integrated machine, and freeze-drying to obtain bovine serum albumin powder.
Preferably, the pressure value in the step S1 is 10 atm.
Preferably, the precipitant in the step S2 is calcium chloride powder, and the calcium chloride powder is mixed with supernatant and centrifugally separated to obtain the bovine serum.
Preferably, the device bovine serum albumin preparation all-in-one, including pretreatment chamber, reaction chamber, vibration dialysis separation chamber, exchange chamber and freeze-drying room, be provided with preprocessing device in the pretreatment chamber, be provided with reaction unit in the reaction chamber, be provided with vibration dialysis separation device in the vibration dialysis separation chamber, exchange chamber is connected with the freeze-drying room.
Preferably, the pretreatment device comprises a centrifugal separator, the centrifugal separator is arranged in a pretreatment chamber, a discharge hole is formed in the bottom end of the centrifugal separator, a sealing cover plate is arranged at the top end of the pretreatment chamber, a pressure sensor and a lifting motor are arranged on the sealing cover plate, a suction pipe is arranged at the bottom end of a lifting rod of the lifting motor, the suction pipe is connected with a reaction chamber through a suction pump, a pressurizing device is arranged on one side of the pretreatment chamber, and an infrared sensor is arranged on the suction pipe.
Preferably, the reaction device comprises a stirring motor, the stirring motor is arranged at the top end of the reaction chamber, a stirring rod of the stirring motor is arranged in the reaction chamber, a feeding box is arranged at one side of the top end of the reaction chamber, an electromagnetic valve is arranged on a feeding port at the bottom end of the feeding box, the bottom end of the reaction chamber is connected with a vibration dialysis separation chamber through a connecting pipe, a filter layer is arranged on the connecting pipe, and an electromagnetic valve is also arranged on the connecting pipe.
Preferably, the vibration dialysis separation device comprises a vibration motor, the vibration motor is arranged at the bottom end of the vibration dialysis separation chamber, a hydraulic cylinder is arranged at the top end of the vibration dialysis separation chamber, a pressing plate is arranged at the bottom end of a hydraulic rod of the hydraulic cylinder, and the pressing plate is in sealing sliding connection with the vibration dialysis separation chamber.
Preferably, a medicine box is further arranged on one side of the vibration dialysis separation chamber, a medicine outlet of the medicine box is connected with the vibration dialysis separation chamber, an electromagnetic valve is also arranged on the medicine outlet, and a dialysis layer is arranged at the bottom end of the vibration dialysis separation chamber.
Compared with the prior art, the invention has the beneficial effects that:
the invention belongs to the technical field of bovine serum albumin manufacturing equipment, and particularly discloses a preparation method and a device for separating bovine serum albumin from bovine serum.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of a pretreatment device according to the present invention;
FIG. 3 is a schematic view of a reaction apparatus of the present invention;
FIG. 4 is a schematic diagram of a vibratory dialysis separator of the present invention;
in the figure: 1. a pretreatment chamber; 2. a reaction chamber; 3. vibrating the dialysis separation chamber; 4. an exchange chamber; 5. a lyophilization chamber; 6. a pretreatment device; 7. a reaction device; 8. a vibration dialysis separation device; 9. a centrifuge; 10. a discharge port; 11. sealing the cover plate; 12. a pressure sensor; 13. a lifting motor; 14. a lifting rod; 15. a suction tube; 16. a suction pump; 17. a pressurizing device; 18. an infrared sensor; 19. a stirring motor; 20. a stirring rod; 21. a charging box; 22. a feed inlet; 23. an electromagnetic valve; 24. a connecting pipe; 25. a filter layer; 26. a vibration motor; 27. a hydraulic cylinder; 28. a hydraulic rod; 29. a pressing plate; 30. a medicine box; 31. a medicine outlet; 32. dialysis layer.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the azimuth or positional relationship indicated by the terms "vertical", "upper", "lower", "horizontal", etc. are based on the azimuth or positional relationship shown in the drawings, and are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the apparatus or element referred to must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present invention.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.
Referring to fig. 1-4, the present invention provides a technical solution: a method for separating bovine serum albumin from bovine serum, comprising the steps of:
s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in a bovine serum albumin preparation integrated machine, pressurizing to crush red blood cells in the bovine blood, and performing centrifugal separation to obtain supernatant;
s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin manufacturing integrated machine, adding a precipitant, and separating to obtain bovine serum;
s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber of a bovine serum albumin manufacturing integrated machine, adding PBS solution into the bovine serum, uniformly mixing, and performing vibration dialysis separation to obtain a solution;
s4, introducing the solution in the step S3 into an exchange chamber in a bovine serum albumin manufacturing all-in-one machine, and obtaining the refined bovine serum albumin through an anion exchange method;
and S5, introducing the refined bovine serum albumin obtained in the step S4 into a freeze-drying chamber in a bovine serum albumin manufacturing integrated machine, and freeze-drying to obtain bovine serum albumin powder.
Further, the pressure value in the step S1 is 10 atm.
Furthermore, the precipitant in the step S2 is calcium chloride powder, and the calcium chloride powder is mixed with supernatant fluid for centrifugal separation to obtain the bovine serum.
Further, the device bovine serum albumin preparation all-in-one, including pretreatment chamber 1, reaction chamber 2, vibration dialysis separation chamber 3, exchange chamber 4 and freeze-drying room 5, be provided with preprocessing device 6 in the pretreatment chamber 1, be provided with reaction unit 7 in the reaction chamber 2, be provided with vibration dialysis separation device 8 in the vibration dialysis separation chamber 3, exchange chamber 4 is connected with freeze-drying room 5.
Further, the pretreatment device 6 comprises a centrifugal separator 9, the centrifugal separator 9 is arranged in the pretreatment chamber 1, a discharge hole 10 is formed in the bottom end of the centrifugal separator 9, a sealing cover plate 11 is arranged at the top end of the pretreatment chamber 1, a pressure sensor 12 and a lifting motor 13 are arranged on the sealing cover plate 11, a suction pipe 15 is arranged at the bottom end of a lifting rod 14 of the lifting motor 13, the suction pipe 15 is connected with the reaction chamber 2 through a suction pump 16, a pressurizing device 17 is arranged on one side of the pretreatment chamber 1, and an infrared sensor 18 is arranged on the suction pipe 15.
Further, the reaction device 7 comprises a stirring motor 19, the stirring motor 19 is arranged at the top end of the reaction chamber 2, a stirring rod 20 of the stirring motor 19 is arranged in the reaction chamber 2, a feeding box 21 is arranged at one side of the top end of the reaction chamber 2, an electromagnetic valve 23 is arranged on a feeding port 22 at the bottom end of the feeding box 21, the bottom end of the reaction chamber 2 is connected with the vibration dialysis separation chamber 3 through a connecting pipe 24, a filter layer 25 is arranged on the connecting pipe 24, and an electromagnetic valve 23 is also arranged on the connecting pipe 24.
Further, the vibration dialysis separation device 8 comprises a vibration motor 26, the vibration motor 26 is installed at the bottom end of the vibration dialysis separation chamber 3, a hydraulic cylinder 27 is arranged at the top end of the vibration dialysis separation chamber 3, a pressing plate 29 is arranged at the bottom end of a hydraulic rod 28 of the hydraulic cylinder 27, and the pressing plate 29 is in sealing sliding connection with the vibration dialysis separation chamber 3.
Further, a medicine box 30 is further arranged on one side of the vibration dialysis separation chamber 3, a medicine outlet 31 of the medicine box 30 is connected with the vibration dialysis separation chamber 3, an electromagnetic valve 23 is also arranged on the medicine outlet 31, and a dialysis layer 32 is arranged at the bottom end of the vibration dialysis separation chamber 3.
Working principle: the invention relates to a preparation method and apparatus for separating bovine serum albumin from bovine serum, in use, add bovine serum albumin into centrifugal separator 9 in the preconditioning room 1, then pressurize preconditioning room 1 through the pressurizing device 17, make the pressure value reach 10 standard atmospheric pressures, guarantee the red blood cell can break completely, make the protein in the red blood cell flow out, then carry on centrifugal separation to bovine serum through the centrifugal separator 9 work, laminate after standing, drive the lifting rod 14 to descend through the work of the lifting motor 13, make the suction tube 15 insert in the supernatant, then the suction pump 16 work draws the supernatant into the reaction chamber 2, the infrared sensor 18 set up on the suction tube 15 can distinguish the stratification of the supernatant, prevent the suction tube 15 from excessively drawing;
introducing into the reaction chamber 3, opening an electromagnetic valve 23 arranged on a charging port 22 on a charging box 21, adding a precipitant into the supernatant, driving a stirring rod 20 to rotate by the operation of a stirring motor 19 to stir and mix the precipitant, obtaining bovine serum after mixing by a filter layer 25, and introducing the bovine serum into the vibration dialysis separation chamber 3;
PBS solution is added into a medicine box 30 in the vibration dialysis separation chamber 3, the vibration motor 26 works to drive the vibration dialysis separation chamber 3 to vibrate, so that bovine serum and the PBS solution are mixed, then the mixed solution is dialyzed through a dialysis layer 32, a hydraulic rod 28 and a pressing plate 29 are pushed to descend by the working of a hydraulic cylinder 27 matched with the dialysis layer to filter-press the mixed solution, the dialysis efficiency is improved, the dialyzed solution enters an exchange chamber 4 to obtain refined bovine serum albumin through an anion exchange method, and then bovine serum albumin powder is obtained through a freeze-drying chamber 5.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. An apparatus for separating bovine serum albumin from bovine serum, comprising: the device is a bovine serum albumin manufacturing integrated machine and comprises a pretreatment chamber (1), a reaction chamber (2), a vibration dialysis separation chamber (3), an exchange chamber (4) and a freeze-drying chamber (5), wherein a pretreatment device (6) is arranged in the pretreatment chamber (1), a reaction device (7) is arranged in the reaction chamber (2), a vibration dialysis separation device (8) is arranged in the vibration dialysis separation chamber (3), and the exchange chamber (4) is connected with the freeze-drying chamber (5);
the pretreatment device (6) comprises a centrifugal separator (9), the centrifugal separator (9) is arranged in a pretreatment chamber (1), a discharge hole (10) is formed in the bottom end of the centrifugal separator (9), a sealing cover plate (11) is arranged at the top end of the pretreatment chamber (1), a pressure sensor (12) and a lifting motor (13) are arranged on the sealing cover plate (11), a suction pipe (15) is arranged at the bottom end of a lifting rod (14) of the lifting motor (13), the suction pipe (15) is connected with a reaction chamber (2) through a suction pump (16), a pressurizing device (17) is arranged on one side of the pretreatment chamber (1), and an infrared sensor (18) is arranged on the suction pipe (15);
the reaction device (7) comprises a stirring motor (19), the stirring motor (19) is arranged at the top end of the reaction chamber (2), a stirring rod (20) of the stirring motor (19) is arranged in the reaction chamber (2), a feeding box (21) is arranged at one side of the top end of the reaction chamber (2), an electromagnetic valve (23) is arranged on a feeding hole (22) at the bottom end of the feeding box (21), the bottom end of the reaction chamber (2) is connected with the vibration dialysis separation chamber (3) through a connecting pipe (24), a filter layer (25) is arranged on the connecting pipe (24), and an electromagnetic valve (23) is also arranged on the connecting pipe (24);
the vibration dialysis separation device (8) comprises a vibration motor (26), the vibration motor (26) is arranged at the bottom end of the vibration dialysis separation chamber (3), a hydraulic cylinder (27) is arranged at the top end of the vibration dialysis separation chamber (3), a pressing plate (29) is arranged at the bottom end of a hydraulic rod (28) of the hydraulic cylinder (27), and the pressing plate (29) is in sealing sliding connection with the vibration dialysis separation chamber (3);
the medicine box is characterized in that a medicine box (30) is further arranged on one side of the vibration dialysis separation chamber (3), a medicine outlet (31) of the medicine box (30) is connected with the vibration dialysis separation chamber (3), an electromagnetic valve (23) is also arranged on the medicine outlet (31), and a dialysis layer (32) is arranged at the bottom end of the vibration dialysis separation chamber (3).
2. A method for separating bovine serum albumin from bovine serum using the apparatus of claim 1, comprising the steps of:
s1, taking a proper amount of fresh bovine blood, putting the fresh bovine blood into a pretreatment chamber in a bovine serum albumin preparation integrated machine, pressurizing to crush red blood cells in the bovine blood, and performing centrifugal separation to obtain supernatant;
s2, introducing the supernatant obtained in the step S1 into a reaction chamber in a bovine serum albumin manufacturing integrated machine, adding a precipitator, stirring and mixing, and filtering by a filter layer to obtain bovine serum;
s3, introducing the bovine serum in the step S2 into a vibration dialysis separation chamber of a bovine serum albumin preparation integrated machine, adding PBS solution into the medicine box, uniformly mixing the bovine serum and the PBS solution, and dialyzing through a dialysis layer to obtain a solution;
s4, introducing the solution in the step S3 into an exchange chamber in a bovine serum albumin manufacturing all-in-one machine, and obtaining the refined bovine serum albumin through an anion exchange method;
and S5, introducing the refined bovine serum albumin obtained in the step S4 into a freeze-drying chamber in a bovine serum albumin manufacturing integrated machine, and freeze-drying to obtain bovine serum albumin powder.
3. The method for separating bovine serum albumin from bovine serum according to claim 2, wherein: the pressure value in the step S1 is 10 standard atmospheres.
4. The method for separating bovine serum albumin from bovine serum according to claim 2, wherein: the precipitant in the step S2 is calcium chloride powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011479957.0A CN112592399B (en) | 2020-12-15 | 2020-12-15 | Preparation method and device for separating bovine serum albumin from bovine serum |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011479957.0A CN112592399B (en) | 2020-12-15 | 2020-12-15 | Preparation method and device for separating bovine serum albumin from bovine serum |
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| CN112592399A CN112592399A (en) | 2021-04-02 |
| CN112592399B true CN112592399B (en) | 2024-04-12 |
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| CN114100242A (en) * | 2021-10-29 | 2022-03-01 | 南京国青血液净化科技有限公司 | Double filtering device for centrifugal hemofiltration treatment |
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|---|---|---|---|---|
| CN1191545A (en) * | 1995-05-25 | 1998-08-26 | 达尔塔生物工艺有限公司 | Process of high purity albumin production |
| EP1785431A1 (en) * | 2004-08-30 | 2007-05-16 | Toray Industries, Inc. | Fractionation apparatus |
| CN108864278A (en) * | 2018-07-27 | 2018-11-23 | 珠海宝锐生物科技有限公司 | A method of preparing molecular biosciences grade bovine serum albumin(BSA) |
| CN210831052U (en) * | 2019-09-11 | 2020-06-23 | 上海欣泽牧生物科技有限公司 | Plastic pad foot dismouting structure and cerini dialyser cerini is used in bovine serum albumin production thereof |
-
2020
- 2020-12-15 CN CN202011479957.0A patent/CN112592399B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1191545A (en) * | 1995-05-25 | 1998-08-26 | 达尔塔生物工艺有限公司 | Process of high purity albumin production |
| EP1785431A1 (en) * | 2004-08-30 | 2007-05-16 | Toray Industries, Inc. | Fractionation apparatus |
| CN108864278A (en) * | 2018-07-27 | 2018-11-23 | 珠海宝锐生物科技有限公司 | A method of preparing molecular biosciences grade bovine serum albumin(BSA) |
| CN210831052U (en) * | 2019-09-11 | 2020-06-23 | 上海欣泽牧生物科技有限公司 | Plastic pad foot dismouting structure and cerini dialyser cerini is used in bovine serum albumin production thereof |
Non-Patent Citations (3)
| Title |
|---|
| Purification and Characterization of Bovine Serum Albumin Using Chromatographic Method;Sanaz Balkani;《Advanced Pharmaceutical Bulletin》;第6卷(第4期);第651-654页 * |
| 牛血清白蛋白纯化工艺的改进;徐云远;《甘肃科学学报》;第3卷(第1期);第51-54页 * |
| 高纯牛血清白蛋白的纯化制备及两种血清蛋白的定值研究;何程铖;《中国优秀硕士学位论文全文数据库基础科学辑》;A006-141 * |
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