CN112587457B

CN112587457B - Method for preparing anti-aging mask

Info

Publication number: CN112587457B
Application number: CN202011573369.3A
Authority: CN
Inventors: 陶虹
Original assignee: Guangzhou Sincerity Cosmetic Co ltd
Current assignee: Guangzhou Sincerity Cosmetic Co ltd
Priority date: 2020-12-25
Filing date: 2020-12-25
Publication date: 2022-04-29
Anticipated expiration: 2040-12-25
Also published as: CN112587457A

Abstract

The invention discloses a preparation method of an anti-aging mask, which comprises the following steps: adding deionized water into a container, adding a thickening agent and liquid grease under the stirring action, homogenizing and stirring until the thickening agent and the liquid grease are completely dispersed, heating, and adding a humectant; cooling the above components, adding antiseptic and deionized water, stirring and mixing; adjusting the pH value according to the formula proportion; performing rotary evaporation on the components, and controlling the viscosity to obtain the emulsion for cosmetics; and adding the base cloth into the emulsion for cosmetics, soaking, and taking out to obtain the facial mask. Wherein the cosmetic emulsion comprises humectant containing beta-glucosidase modified sodium alginate, propylene glycol, semen Cucurbitae extract, polyvinylpyrrolidone, arachidyl alcohol propionate, and water soluble ascorbic acid derivative; the anti-aging mask has a good net structure, a high free radical removal rate, a high tyrosinase inhibition rate, and good anti-aging, water retention and anti-irritation effects.

Description

Method for preparing anti-aging mask

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a preparation method of an anti-aging mask.

Background

The skin is the largest organ of the human body and ages with age. In general, wrinkles are long, with progressive pigmentation, and the skin then becomes dry, loose, rough, dull, while the tactile, pain and temperature sensations of the skin are diminished, the reactivity of the surface is attenuated, disordered, regenerated and healed and can be easily stimulated. It is seen that skin aging is accompanied by the growth of a half of a person, which negatively affects the work and life of people who love beauty, and there is a great demand for cosmetics against aging in the world, particularly in affluent countries. Delaying skin aging is one of research focuses of cosmetics, but at present, research on anti-aging cosmetic raw materials is less, anti-aging cosmetic raw materials provided in the market are not rich enough, and research and development of anti-aging cosmetics are limited.

The prior art, for example, application No. 2017113375697 discloses a cellulose nanowhisker antibacterial moisturizing cream-type mask, a preparation method and an application thereof, wherein the preparation method comprises the following steps: the weight percentage of the material is as follows: 1-20% of cellulose nanocrystal, 0.5-3% of chitosan, 1-5% of polyvinyl alcohol, 0.1-2% of sodium alginate, 0.1-2% of propylene glycol, 1-3% of glycerol, 0.1-2% of silicon emulsion, 0.1-1% of aloe extract, 0.5-2% of ethanol and the balance of water. The cellulose nanowhisker antibacterial moisturizing cream type facial mask can solve the problems of inconvenient treatment after the facial mask is used, poor moisturizing effect and unobvious whitening in the prior art; the water-retaining facial mask has good moisture retention, realizes deep and rapid water replenishing, has excellent anti-aging and antibacterial properties, and can be applied to a plurality of application fields such as facial mask materials, daily chemical products and the like. Application No. 2014103701183 discloses a traditional Chinese medicine facial mask, which comprises radix Angelicae sinensis, rhizoma Atractylodis Macrocephalae, rhizoma Bletillae, radix astragali, rhizoma Ligustici Chuanxiong, Spirulina, semen Vaccariae and barley green. The components in the traditional Chinese medicine mask can generate mutual incompatibility, have no side effect of mutual drug property conflict, can radically improve the functions of expelling toxin and nourishing skin white, restore the due vitality of the skin, have good nourishing effect, can improve the skin quality, and cannot generate dependence. And no chemical preservative is needed to be added, the self characteristic of the formula can play a role in corrosion prevention, and the use is safe.

Disclosure of Invention

The invention aims to provide an anti-aging mask which has a good net structure inside, a high free radical removal rate, a high tyrosinase inhibition rate, good anti-aging property, water retention property and an anti-irritation effect.

The technical scheme adopted by the invention for realizing the purpose is as follows:

a cosmetic lotion comprises humectant containing beta-glucosidase modified sodium alginate, propylene glycol, semen Cucurbitae extract, polyvinylpyrrolidone, arachidonol propionate, and water soluble ascorbic acid derivative;

the beta-glucosidase modified sodium alginate is prepared by solidifying beta-glucosidase on sodium alginate.

The beta-glucosidase modified sodium alginate and the pumpkin seed extract are used as components of the emulsion for cosmetics, and can play a role in scavenging free radicals after being absorbed by the epidermis of a human body, so that bioactive macromolecules such as protein, lipid and the like are prevented from being inactivated after being attacked by the free radicals, damage of the free radicals to the structure and the function of the skin is reduced, and the aging is delayed; in addition, the emulsion for cosmetics has a good net structure inside, and all components are loaded in the net structure and are contacted with skin, so that on one hand, the water retention of the skin can be increased, and the skin is enabled to be more bright and white; on the other hand, the effects of remarkably improving the water content of the skin, reducing the water loss of the skin and improving the elasticity of the skin can be achieved so as to achieve the aim of anti-aging effect; meanwhile, the beta-glucosidase modified sodium alginate and the pumpkin seed extract in the emulsion for the cosmetics interact with other components, so that the antioxidant stability of the skin can be improved, the activity of tyrosinase can be inhibited, the formation of melanin is prevented, the darkness of the skin is improved, and the purpose of whitening is achieved; and has good anti-irritation effect and no toxic or side effect on human body.

Preferably, the recovery rate of the enzyme activity in the beta-glucosidase modified sodium alginate is higher than 80.2%.

Preferably, the humectant comprises, by weight, 0.4-5 parts of beta-glucosidase modified sodium alginate, 2-8 parts of propylene glycol, 0.5-3 parts of pumpkin seed extract, 0.02-2.5 parts of polyvinylpyrrolidone, 0.6-4.5 parts of arachidonol propionate and 0.01-5 parts of water-soluble ascorbic acid derivative.

Preferably, the preparation method of the pumpkin seed extract comprises the following steps:

weighing 1.5-3.5 parts by weight of pumpkin seed powder, placing the pumpkin seed powder into a filter paper hopper, placing the filter paper hopper into a Soxhlet extractor, adding petroleum ether, extracting for 8-12 hours at 65-75 ℃, concentrating and recovering by using a rotary evaporator, and drying the grease, wherein the extraction rate is 51.2-53.6%.

Preferably, the water-soluble ascorbic acid derivative is one or a mixture of L-sodium ascorbate, L-magnesium ascorbyl phosphate, L-calcium ascorbyl phosphate or L-sodium ascorbyl sulfate.

Preferably, the beta-glucosidase modified sodium alginate and propylene glycol, pumpkin seed extract are used for removing free radicals.

Preferably, the beta-glucosidase modified sodium alginate, propylene glycol and pumpkin seed extract are used for increasing the water retention of skin.

Preferably, the cosmetic emulsion is used in a facial mask.

Preferably, the cosmetic emulsion is used for the antioxidant stability of skin and/or inhibiting the activity of tyrosinase.

Preferably, the cosmetic emulsion also comprises a thickening agent, liquid grease, a preservative and the balance of deionized water.

More preferably, the thickening agent is one or a mixture of lauric acid, linoleum diethanolamide or myristyl alcohol.

More preferably, the liquid oil is one or a mixture of lecithin, candelilla wax or vitamin A acetate.

More preferably, the preservative is one or a mixture of benzyl alcohol, sorbic acid, salicylic acid or p-hydroxyacetophenone.

The invention also aims to prepare the anti-aging mask with higher free radical removal rate, tyrosinase inhibition rate, better anti-aging property, water retention property and anti-irritation effect.

a preparation method of the anti-aging mask comprises the following steps:

s1: adding deionized water into a container, adding a thickening agent and liquid grease under the stirring action, homogenizing and stirring until the thickening agent and the liquid grease are completely dispersed, heating to 75-85 ℃, and adding a humectant;

s2: cooling the components in the step S1 to 30-40 ℃, adding a preservative and deionized water, and stirring and mixing uniformly;

s3: weighing a pH regulator according to a formula ratio, adding the pH regulator into a container, and regulating the pH to 6.1-6.4;

s4: rotationally steaming the components in the step S3, and controlling the viscosity to obtain the emulsion for cosmetics;

s5: and adding the base cloth into the emulsion for cosmetics to be soaked, taking out and carrying out vacuum packaging to obtain the facial mask.

The cosmetic emulsion is combined with other components to obtain a facial mask, the facial mask is contacted with human skin, and after being absorbed by human epidermis, the facial mask can remove free radicals, improve the oxidation resistance of the skin and further delay aging; in addition, the mask can enable the skin to have better water retention, promote the skin to be more white and bright, and simultaneously obviously improve the effects of increasing the water content of the skin, reducing the water loss of the skin and improving the elasticity of the skin so as to achieve the aim of resisting aging; the components in the anti-aging mask interact with each other, so that on one hand, the anti-oxidation stability is improved, and on the other hand, the activity of tyrosinase is inhibited, and the purpose of whitening is achieved; meanwhile, the facial mask has good anti-irritation effect and no toxic or side effect on a human body.

Preferably, in the anti-aging mask, by weight, the humectant is 3.85-25 parts, the thickener is 0.2-1.5 parts, the liquid oil is 10-18 parts, the preservative is 0.15-0.95 part, the pH regulator is 0.02-2.5 parts, and the balance is deionized water.

Preferably, the speed of homogeneous stirring is 45-75 r/min.

Preferably, the pH adjusting agent is triethanolamine.

Preferably, the cosmetic emulsion has a viscosity of 2500 to 6000mpa · s.

Preferably, the base cloth is one of cotton cloth, tencel or silk.

Preferably, the base cloth is soaked in 20-50 parts by weight of the emulsion for cosmetics for 5-15 min.

In order to further improve the free radical removal rate, the water retention property, the anti-aging property and the tyrosinase inhibition rate of the anti-aging mask, the preferable measures further comprise:

0.05-0.2 part by weight of polyacrylamide/attapulgite particles are added into the emulsion for cosmetics, the polyacrylamide/attapulgite particles can be crosslinked with each component in the emulsion for cosmetics to a certain degree, the water retention of the mask is improved, meanwhile, the polyacrylamide/attapulgite particles and beta-glucosidase modified sodium alginate can further form a net structure, other components are more easily attached to the surface of the net structure and are in full contact with the skin, the effects of better removing free radicals, resisting aging and inhibiting the activity of tyrosinase are achieved, and the effects of resisting oxidation and aging, supplementing water and whitening of the mask are further improved.

Preferably, the preparation method of the polyacrylamide/attapulgite particles comprises the following steps:

dissolving monomer acrylamide, cross-linking agent N, N ' -methylene bisacrylamide, initiator potassium persulfate and accelerator N, N, N ', N ' -tetramethyl ethylenediamine in distilled water, adding attapulgite in batches, wherein the mass ratio of the monomer acrylamide to the attapulgite is 1: 1-3, the content of the cross-linking agent is 0.01-0.015% of the total amount of reactants, the content of the initiator is 0.5-1.5% of the total amount of the reactants, fully mixing uniformly, standing at room temperature until the cross-linking polymerization is completed, drying at 70-80 ℃, crushing and screening for later use after 0.1-0.2 mm.

The invention adopts beta-glucosidase modified sodium alginate and pumpkin seed extract as the components of the emulsion for cosmetics, and combines with other components to obtain the anti-aging mask, thereby having the following beneficial effects: the anti-aging mask can play a role in scavenging free radicals after contacting with skin so as to reduce the damage of the free radicals to the structure and the function of the skin, thereby delaying aging; in addition, the mask can enable the skin to have better water retention, promote the skin to be more white and bright, and simultaneously obviously improve the effects of increasing the water content of the skin, reducing the water loss of the skin and improving the elasticity of the skin so as to achieve the aim of resisting aging; the cosmetic emulsion in the anti-aging mask interacts with other components, so that on one hand, the anti-oxidation stability is improved, on the other hand, the activity of tyrosinase is inhibited, and the purpose of whitening is achieved; meanwhile, the facial mask has good anti-irritation effect and no toxic or side effect on a human body. Therefore, the anti-aging mask has high free radical removal rate and tyrosinase inhibition rate, and good anti-aging property, water retention property and anti-irritation effect.

Drawings

FIG. 1 is an SEM image of a cosmetic emulsion;

FIG. 2 is a graph showing the inhibition of RBC hemolysis rate caused by SDS of the anti-aging mask;

fig. 3 is a free radical removal rate of the anti-aging mask;

fig. 4 is a water retention rate of the anti-aging mask;

FIG. 5 is the tyrosinase inhibition rate of the anti-aging mask;

fig. 6 is the antioxidant stability of the anti-aging mask.

Detailed Description

The experimental methods described in the following examples of the present invention are all conventional methods unless otherwise specified; the reagents and materials are commercially available, unless otherwise specified.

Further, in some embodiments, the pumpkin seed extract is prepared by the following method:

weighing 2 parts by weight of pumpkin seed powder, placing the pumpkin seed powder into a filter paper hopper, placing the filter paper hopper into a Soxhlet extractor, adding petroleum ether, extracting for 10 hours at 75 ℃, concentrating and recovering by using a rotary evaporator, and drying oil with the extraction rate of 51.6%.

Further, in some embodiments, the β -glucosidase modified sodium alginate is prepared as follows:

preparing 20-25U/mL enzyme solution from beta-glucosidase by using acetic acid buffer solution with pH value of 4.8-5.2, and storing at 2-4 ℃ for later use; adding the enzyme solution into an isovolumetric glutaraldehyde solution with the concentration of 0.15-0.25% for crosslinking for 1-3 min, marking as a solution a, then adding the solution a into a sodium alginate solution with the mass concentration of 10-20 g/L, wherein the volume ratio of the solution a to the sodium alginate is 1: 4-6, and uniformly stirring; sucking the mixed solution by an injector and dropwise adding CaCl with the mass concentration of 2-10 g/L₂And (3) solidifying the solution for 1-2 h, taking out, and washing for 2-4 times by using an acetic acid buffer solution with the pH value of 4.8-5.2 to obtain the beta-glucosidase modified sodium alginate material, wherein the recovery rate of the enzyme activity is higher than 80.2%.

Further, in some embodiments, a method of making an anti-aging mask, comprising the steps of:

s1: adding deionized water into a container, adding 0.2-1.5 parts by weight of a thickening agent and 10-18 parts by weight of liquid oil under the stirring action, homogenizing and stirring for 10-20 min at the speed of 45-75 r/min until the mixture is completely dispersed, heating to 75-85 ℃, and then adding 3.85-25 parts by weight of a humectant, wherein in the humectant, 0.4-5 parts by weight of beta-glucosidase modified sodium alginate, 2-8 parts by weight of propylene glycol, 0.5-3 parts by weight of pumpkin seed extract, 0.02-2.5 parts by weight of polyvinylpyrrolidone, 0.6-4.5 parts by weight of arachidonol propionate and 0.01-5 parts by weight of water-soluble ascorbic acid derivative are added;

s2: cooling the components in the step S1 to 30-40 ℃, adding deionized water and 0.15-0.95 weight part of preservative, and stirring and mixing uniformly;

s3: weighing 0.02-2.5 parts by weight of pH regulator according to the formula proportion, adding into a container, and regulating the pH to 6.1-6.4;

s4: performing rotary evaporation on the components in the step S3, and controlling the viscosity to be 2500-6000 mpa & S to obtain the emulsion for cosmetics;

s5: and adding the base cloth into 20-50 parts by weight of the cosmetic emulsion, soaking for 5-15 min, taking out, and carrying out vacuum packaging to obtain the mask.

The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:

example 1

A preparation method of the anti-aging mask comprises the following steps:

(1) preparation of beta-glucosidase modified sodium alginate

Preparing 24U/mL enzyme solution from beta-glucosidase by using acetic acid buffer solution with pH value of 5.1, and storing at 3 ℃ for later use; adding the enzyme solution into an isovolumetric glutaraldehyde solution with the concentration of 0.15% for crosslinking for 2min, marking as a solution a, then adding the solution a into a sodium alginate solution with the mass concentration of 15g/L, wherein the volume ratio of the solution a to the sodium alginate is 1:5, and uniformly stirring; sucking the mixed solution by a syringe and dropwise adding CaCl with the mass concentration of 6g/L₂In the solution, the solution is added with a solvent,and taking out after solidification for 1.5h, and washing for 4 times by using acetic acid buffer solution with the pH value of 5.1 to obtain the beta-glucosidase modified sodium alginate material, wherein the recovery rate of the enzyme activity is 83.5%.

(2) Preparation of anti-aging mask

S1: adding deionized water into a container, adding 0.8 weight part of linoleum diethanolamide and 15 weight parts of candelilla wax under the stirring action, homogenizing and stirring for 15min at the speed of 55r/min until the mixture is completely dispersed, heating to 80 ℃, and then adding 17 weight parts of humectant, wherein in the humectant, 3.5 weight parts of beta-glucosidase modified sodium alginate, 4.5 weight parts of propylene glycol, 1.5 weight parts of pumpkin seed extract, 0.09 weight parts of polyvinylpyrrolidone, 2.8 weight parts of arachidonol propionate and 0.15 weight part of L-ascorbic acid magnesium phosphate;

s2: cooling the components in the step S1 to 35 ℃, adding deionized water and 0.52 weight part of benzyl alcohol, and stirring and mixing uniformly;

s3: weighing 1.5 parts by weight of triethanolamine according to the formula proportion, adding into a container, and adjusting the pH value to 6.3;

s4: rotationally steaming the components in the step S3, and controlling the viscosity to be 4000mpa & S to obtain the emulsion for cosmetics;

s5: and adding cotton cloth into 32 parts by weight of the cosmetic emulsion, soaking for 8min, taking out, and carrying out vacuum packaging to obtain the facial mask.

Example 2

A preparation method of the anti-aging mask comprises the following steps:

(1) preparation of beta-glucosidase modified sodium alginate

Preparing the beta-glucosidase into 22U/mL enzyme solution by using acetic acid buffer solution with the pH value of 5.0, and storing the enzyme solution at 4 ℃ for later use; adding the enzyme solution into an isovolumetric glutaraldehyde solution with the concentration of 0.45% for crosslinking for 1min, marking as a solution a, then adding the solution a into a sodium alginate solution with the mass concentration of 20g/L, wherein the volume ratio of the solution a to the sodium alginate is 1:4, and uniformly stirring; sucking the mixed solution by a syringe and dropwise adding CaCl with the mass concentration of 3g/L₂Solidifying for 1 hr, taking out, and buffering with acetic acid buffer solution with pH of 5.0Washing for 3 times to obtain the beta-glucosidase modified sodium alginate material, wherein the enzyme activity recovery rate is 85.1%.

(2) Preparation of anti-aging mask

S1: adding deionized water into a container, adding 1.2 parts by weight of myristyl alcohol and 13 parts by weight of vitamin A acetate under the stirring action, homogenizing and stirring for 20min at the speed of 75r/min until the mixture is completely dispersed, heating to 85 ℃, and then adding 13.5 parts by weight of humectant, wherein in the humectant, 1.8 parts by weight of beta-glucosidase modified sodium alginate, 6.5 parts by weight of propylene glycol, 2.5 parts by weight of pumpkin seed extract, 1.4 parts by weight of polyvinylpyrrolidone, 1.5 parts by weight of arachidonol propionate and 3.5 parts by weight of L-sodium ascorbate;

s2: cooling the components in the step S1 to 38 ℃, adding 0.48 part by weight of salicylic acid and deionized water, and stirring and mixing uniformly;

s3: weighing 0.5 part by weight of triethanolamine according to the formula proportion, adding into a container, and adjusting the pH value to 6.1;

s4: rotationally steaming the components in the step S3, and controlling the viscosity to be 5000mpa & S to obtain the emulsion for cosmetics;

s5: adding silk into 40 parts by weight of cosmetic emulsion, soaking for 5min, taking out, and vacuum packaging to obtain the facial mask.

Examples 3 to 4

A method for preparing an anti-aging mask, wherein the step (1) in the embodiment 3-4 is the same as the embodiment 2, and the difference from the embodiment 2 is that in the step (2), the anti-aging mask comprises the following components in parts by weight, namely a moisturizing agent, myristyl alcohol, vitamin A acetate and salicylic acid, and the specific difference is shown in the table 1.

TABLE 1 difference in parts by weight of each component in examples 2 to 4

Example 5

A method for producing an anti-aging mask, in which in example 5, step (1) is the same as example 2, but in step (2), S1 includes, as humectant components, 3.2 parts by weight of β -glucosidase-modified sodium alginate, 4.5 parts by weight of propylene glycol, 2.6 parts by weight of pumpkin seed extract, 2.4 parts by weight of polyvinylpyrrolidone, 1.9 parts by weight of arachidonoyl propionate, and 0.06 parts by weight of sodium L-ascorbate.

Example 6

In order to further improve the free radical removal rate, the water retention property, the anti-aging property and the tyrosinase inhibition rate of the anti-aging mask, the method for preparing the anti-aging mask further comprises the following steps:

the procedure of step (1) was the same as in example 2;

(2) preparation of anti-aging mask

S1: adding deionized water into a container, adding 1.2 parts by weight of myristyl alcohol, 13 parts by weight of vitamin A acetate and 0.07 part by weight of polyacrylamide/attapulgite particles under the stirring action, homogenizing and stirring for 20min at the speed of 75r/min until the mixture is completely dispersed, heating to 85 ℃, and then adding 13.5 parts by weight of humectant, wherein in the humectant, 1.8 parts by weight of beta-glucosidase modified sodium alginate, 6.5 parts by weight of propylene glycol, 2.5 parts by weight of pumpkin seed extract, 1.4 parts by weight of polyvinylpyrrolidone, 1.5 parts by weight of arachidonol propionate and 3.5 parts by weight of L-sodium ascorbate;

s2: for step S1

The preparation of the polyacrylamide/attapulgite particles comprises the following steps:

dissolving monomer acrylamide, cross-linking agent N, N ' -methylene bisacrylamide, initiator potassium persulfate and accelerator N, N, N ', N ' -tetramethyl ethylenediamine in distilled water, adding attapulgite in batches, wherein the mass ratio of the monomer acrylamide to the attapulgite is 1:2, the content of the cross-linking agent is 0.015 percent of the total amount of reactants, the content of the initiator is 1.2 percent of the total amount of the reactants, fully and uniformly mixing, placing at room temperature until the cross-linking polymerization is completed, drying at 75 ℃, crushing and sieving by 0.15 mm.

Example 7

The other steps of the method for preparing the anti-aging mask are the same as those of the embodiment 6, and the method is different from the embodiment 6 in that: in step (2), at S1, 0.15 parts by weight of polyacrylamide/attapulgite particles were added.

Comparative example 1

The other steps of the method for preparing the anti-aging mask are the same as those of the embodiment 2, and the method is different from the embodiment 2 in that: in step (2) S1, the humectant component is not added with β -glucosidase-modified sodium alginate.

Comparative example 2

The other steps of the method for preparing the anti-aging mask are the same as those of the embodiment 2, and the method is different from the embodiment 2 in that: in S1 of the step (2), the pumpkin seed extract is not added to the humectant component.

Comparative example 3

The other steps of the method for preparing the anti-aging mask are the same as those of the embodiment 2, and the method is different from the embodiment 2 in that: in S1 of step (2), the β -glucosidase modified sodium alginate in the humectant component is replaced with sodium alginate.

Comparative example 4

The other steps of the method for preparing the anti-aging mask are the same as those of the embodiment 2, and the method is different from the embodiment 2 in that: in step (2), in S1, the humectant component does not include the β -glucosidase-modified sodium alginate and the pumpkin seed extract.

Test example 1:

1. determination of surface morphology of emulsion for cosmetics

In the test, XL-30 type electronic Scanning Electron Microscope (SEM) is adopted to observe the surface appearance of the cosmetics.

FIG. 1 is an SEM image of a cosmetic emulsion. As can be seen from figure 1, each component in the cosmetic emulsion has a network cross-linked structure, has a better network structure, and other components can be attached to the surface of the network structure.

2. Determination of anti-irritation of anti-aging mask

This test uses the RBC hemolysis test to determine the irritation of an anti-aging mask. Preparation and storage of Red Blood Cells (RBC) 450mL of fresh bovine blood was taken from a slaughterhouse, filled in a polyethylene plastic container, and mixed with 50mL of citric acid buffer. Immediately keeping the mixed blood sample in a thermal insulation box at the temperature of 21-22 ℃, transporting the mixed blood sample to a laboratory within 30min, and prolonging the time to 1h if the blood sample is not polluted. A1.0 mg/mL SDS solution was prepared in PBS. To a 1.5mL EP tube, 20. mu.L of SDS solution was added, and then 975. mu.L of PBS was added. Add 25. mu.L of RBC suspension quickly and incubate at 37 ℃ for 10min with shaking at 150 r/min. The reaction was stopped by centrifugation at 11180 Xg (about 10000r/min) for 1min, and the supernatant was measured for absorbance at 530nm in a 1cm cuvette. Simultaneously observing the morphology of the red blood cells and the integrity of the cell membrane under a microscope; comparison of the positive control group and the sample + SDS group can characterize the anti-irritation properties of the sample. The hemolysis rate is calculated as:

hemolysis rate (%) - (A)_{Test tube}-A_{Self-dissolving tube})/(A_{Full-dissolving tube}-A_{Self-dissolving tube})×100％

In the formula:

A_{test tube}-absorbance values at 530nm for the SDS set;

A_{self-dissolving tube}-absorbance values at 530nm for RBC autolytic control;

A_{full-dissolving tube}Absorbance values at 530nm for the positive control group.

The stimulation of the sample was reflected by measuring the extent to which the sample inhibited damage to blood cells caused by SDS using SDS as a stimulus.

Fig. 2 is a graph showing the inhibition of RBC hemolysis rate caused by SDS by the anti-aging mask. As can be seen from fig. 2, the inhibition rate of RBC hemolysis caused by SDS is higher than 83% in examples 1-5, the inhibition rate of RBC hemolysis caused by SDS is higher in comparative example 2 and comparative examples 1-4, and the inhibition rate of RBC hemolysis caused by SDS is higher in example 2 than in comparative examples 1-4, which shows that the addition of the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract to the anti-aging mask can improve the inhibition rate of RBC hemolysis caused by SDS of the mask, i.e., improve the anti-irritation property of the mask; the RBC hemolysis inhibition rate by SDS of examples 6-7 is higher than 86%, comparing example 2 with example 6, the RBC hemolysis inhibition rate by SDS of example 6 is higher than example 2, which shows that the RBC hemolysis inhibition rate by SDS of the facial mask can be further improved by adding polyacrylamide/attapulgite particles into the cosmetic emulsion and using the cosmetic emulsion in the anti-aging facial mask.

3. Determination of anti-aging mask free radical removal rate

Accurately weighing 112mg DPPH, dissolving in anhydrous ethanol, diluting to 100mL, taking 2.0mL of the solution, and measuring A at 519nm_oThe value is obtained. The absorbance A of the mixture of 1mL sample and 3mL DPPH solution was measured by the same method_tAnd absorbance A of 1mL sample mixed with 3mL absolute ethyl alcohol_bThe DPPH · removal rate (S) calculation formula is as follows:

removal rate (S) ═ 1- ((A)_t-A_b)/A_o)×100％

Fig. 3 shows the radical removal rate of the anti-aging mask. As can be seen from fig. 3, the radical removal rate of examples 1 to 5 is not less than 94%, the radical removal rate of comparative example 2 and comparative examples 1 to 4 is higher than that of comparative examples 1 to 4, which shows that the addition of the cosmetic emulsion containing both the beta-glucosidase modified sodium alginate and the pumpkin seed extract to the anti-aging facial mask improves the radical removal rate of the facial mask, because the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract in the facial mask can exert the radical scavenging effect after being absorbed by the epidermis of the human body, thereby delaying aging; the radical removal rate of the facial mask is higher than 97% in the examples 6 and 7, and the radical removal rate of the facial mask is higher than that of the facial mask in the comparative examples 2 and 6 and the radical removal rate of the facial mask in the example 6 is higher than that of the facial mask in the example 2, which shows that the polyacrylamide/attapulgite particles added to the facial mask can be crosslinked with each component in the cosmetic emulsion to a certain extent, other components are more easily attached to the surface of the net structure and are fully contacted with the skin, and the radical removal rate is better, so that the radical removal rate of the facial mask is further improved.

4. Determination of anti-aging mask water retention rate

The mass of the mask base cloth is recorded as m₁Adding 40g of cosmetic lotion, soaking for 5min, taking out the mask base cloth, weighing unabsorbed lotion m₂The mass of the liquid of the mask base cloth is m₃(m₃＝40-m₂) Applying the facial mask on the face of the subject for 15min, taking off, and weighing₄And z is the water retention rate B of the mask, and the calculation formula is as follows:

water retention (B) 1- (m)₁+40-m₄)/m₃×100％

Fig. 4 is a water retention rate of the anti-aging mask. As can be seen from fig. 4, the water retentions of examples 1 to 5 were higher than 92.5%, the water retentions of comparative examples 2 and comparative examples 1 to 4, and the water retentions of example 2 were higher than those of comparative examples 1 to 4, which indicates that the water retentions of the facial mask were improved by adding the cosmetic emulsion containing the β -glucosidase-modified sodium alginate and the pumpkin seed extract to the facial mask; the cosmetic emulsion has a good net-shaped internal structure, and all components are loaded in the net-shaped structure and are in contact with the skin, so that the skin can achieve the water retention effect, the water retention rate of the skin can be increased, and the skin is enabled to be more white and bright; the water retentions of examples 6 and 7 were higher than 95%, and the water retentions of comparative examples 2 and 6 were higher than those of example 2, which shows that the addition of polyacrylamide/attapulgite fine particles to the facial mask can cause physicochemical reactions with the respective components of the cosmetic emulsion, and improve the water retentivity of the facial mask.

5. Determination of anti-aging property of anti-aging mask

The parameters of skin condition (including skin moisture content, transdermal moisture loss, skin elasticity) before (0 h) and after (4 h, 8h) use of volunteers were determined using a multifunctional skin tester.

Skin moisture content: skin moisture content testing was performed using the Corneometer CM825 probe of a multifunctional skin tester. The probe reflects the change of skin moisture by measuring the capacitance value of the skin, and the higher the CM value is, the higher the moisture content of the stratum corneum is; specific test group and control group CM_PThe test data are shown in table 1;

percutaneous water loss: miningThe moisture loss test probe of the multifunctional skin tester is used for carrying out the percutaneous moisture loss test. The higher the transdermal water loss value (TEWL value), indicating that the more water is lost through the skin, the poorer the barrier function of the stratum corneum; specific test and control groups TEWL_PThe test data are shown in Table 2;

skin elasticity: skin elasticity test is carried out by adopting a skin elasticity probe of the multifunctional skin tester, and the skin elasticity test returns to R₂Value data. R₂The values are the overall elasticity parameter, with larger values indicating better skin elasticity; the test data of the test group and the control group are shown in Table 3.

Table 1 moisturizing efficacy data

Table 1 shows moisturizing efficacy data of the anti-aging mask. As can be seen from Table 1, after the 4h and the 8h of the mask, the moisture content of the skin is greatly improved compared with the control group in examples 1-5, but the moisture content of the skin is not obviously improved compared with the control group in comparative examples 1-4, the CM average value of example 2 is higher than that of comparative examples 1-4 after the 4h and the 8h of the mask, which shows that the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract is added into the mask, so that the skin moisture content is improved; after the mask was used for 4h and 8h, the CM average values of example 6 and example 7 were greatly increased compared to the control, comparing example 2 with example 6, and the CM average value of example 6 was higher than that of example 2, indicating that the addition of polyacrylamide/attapulgite particles to the mask increased the moisture content of the skin.

Table 2 skin barrier efficacy data

Test group	TEWL mean value (0-4h)	TEWL mean value (0-8h)
			Example 1	-4.02	-2.85
Example 2	-5.13	-3.29
			Example 3	-4.54	-2.94
Example 4	-4.75	-3.07
			Example 5	-5.05	-3.16
Example 6	-5.63	-4.47
			Example 7	-5.27	-4.25
Comparative example 1	-2.58	-1.94
			Comparative example 2	-3.47	-2.23
Comparative example 3	-3.05	-2.16
			Comparative example 4	-1.68	-1.54
Control group TEWL_P	-1.55	-1.28

Table 2 is skin barrier efficacy data. As can be seen from table 2, after the mask was used for 4h and 8h, the skin moisture loss values through skin were decreased in examples 1 to 5 compared to the control group after use, and compared to examples 2 and comparative examples 1 to 4, the average TEWL value of example 2 was lower than that of comparative examples 1 to 4 after the mask was used for 4h and 8h, which indicates that the cosmetic emulsion containing β -glucosidase-modified sodium alginate and pumpkin seed extract significantly improved the skin barrier function by adding thereto; after the mask was used for 4h and 8h, the TEWL average values of example 6 and example 7 were greatly improved compared to the control group, comparing example 2 with example 6, and the TEWL average value of example 6 was lower than that of example 2, which indicates that the addition of polyacrylamide/attapulgite particles to the mask further improves the skin barrier function.

TABLE 3 elasticity improvement efficacy data

Test group	R₂Mean value (0-4h)	R₂Mean value (0-8h)
			Example 1	7.56％	4.83％
Example 2	7.87％	5.22％
			Example 3	7.61％	4.93％
Example 4	7.68％	5.08％
			Example 5	7.73％	5.11％
Example 6	7.94％	5.31％
			Example 7	7.89％	5.24％
Comparative example 1	6.85％	2.37％
			Comparative example 2	7.03％	2.74％
Comparative example 3	6.97％	2.59％
			Comparative example 4	6.67％	2.16％
Control group R_2P	6.65％	1.81％

Table 3 shows elasticity improvement efficacy data of the anti-aging mask. As can be seen from Table 3, after 4h and after 8h of using the mask, R after use of examples 1 to 7 was compared with that of the control group₂The average values are increased to a certain extent, and compared with example 2 and example 6, example 6 is slightly increased compared with example 2, which shows that the addition of the polyacrylamide/attapulgite particles in the facial mask hardly influences the improvement of the skin elasticity of the facial mask and slightly improves the skin elasticity of the facial mask; comparing example 2 with comparative examples 1-4, R for example 2 after 4h and after 8h using the mask₂The average value is higher than that of comparative examples 1 to 4, which shows that the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract is added into the facial mask, so that the skin elasticity can be improved.

As can be seen from the data in tables 1, 2 and 3, the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract is added to the anti-aging mask, so that the effects of remarkably increasing the water content of the skin, reducing the water loss through the skin and improving the elasticity of the skin, namely the anti-aging effect of the mask, can be improved.

6. Determination of tyrosinase inhibition rate of anti-aging mask

PBS (phosphate buffered saline) with pH 6.9, L-tyrosinase solution at 1.0mg/mL and tyrosinase solution at 0.1mg/mL were prepared, respectively. Adding samples according to the following mixture ratio, wherein the sample to be tested is 1:2.0mL of PBS, 0.5mL of tyrosinase solution and 0.5mL of L-tyrosine solution; sample 2 is measured by adding 2.5mL of PBS and 0.5mL of tyrosinase solution; test sample 3, 0.5m sample solution +1.5mL PBS +0.5mL tyrosinase solution +0.5mL L-tyrosine solution; test sample 4:0.5mL sample solution +2.0mL PBS +0.5mL tyrosinase solution. Mixing the test samples, mixing 1-4 kinds of test samples, placing in 37 deg.C water bath for 30min, immediately taking out, and measuring absorbance at 475nm (A)₁-A₄). The tyrosinase inhibition rate (I) of the mask was calculated according to the following formula.

I＝[1-(A₃-A₄)/(A₁-A₂)]×100％

In the formula: a. the₁-A₄To determine the absorbance values at 475nm for samples 1-4.

Fig. 5 is the tyrosinase inhibition rate of the anti-aging mask. As can be seen from fig. 4, the tyrosinase inhibition rates of examples 1 to 5 are higher than 90%, the tyrosinase inhibition rates of comparative example 2 and comparative examples 1 to 4 are higher than those of comparative examples 1 to 4, and this shows that the cosmetic emulsion containing the beta-glucosidase-modified sodium alginate and the pumpkin seed extract is added to the facial mask, so that the tyrosinase inhibition rate of the facial mask is improved; the reason is that the beta-glucosidase modified sodium alginate in the emulsion for the cosmetics and pumpkin seeds interact with other components to inhibit the activity of tyrosinase, so that the formation of melanin is prevented, the skin darkness is improved, and the purpose of whitening is achieved; the tyrosinase inhibition rates of examples 6 and 7 are higher than 94%, and the tyrosinase inhibition rates of comparative examples 2 and 6 and example 6 are higher than that of example 2, which shows that the addition of polyacrylamide/attapulgite particles to the facial mask can cause physicochemical reactions with the components in the cosmetic emulsion, thereby improving the tyrosinase inhibition rate of the facial mask.

7. Determination of anti-oxidation stability of anti-aging mask

And measuring the removal rate of the mask to DPPH free radicals at room temperature every 5d (30 d in total) by adopting a method for measuring the removal rate of the free radicals, and evaluating the stability of the oxidation resistance of the mask according to the change trend of the removal rate.

Fig. 6 is the antioxidant stability of the anti-aging mask. As can be seen from fig. 5, the decrease of the radical removal rate is smaller in examples 2 and 6 with the increase of time, and the decrease of example 6 is almost not significantly different from example 2 in comparison examples 2 and 6, and the radical removal rate is maintained above 90%, which indicates that the antioxidant stability of the mask is hardly affected by adding the polyacrylamide/attapulgite particles to the mask; comparing example 2 with comparative examples 1-4, the radical removal rate of comparative examples 1-4 is more obvious in the reduction trend, which shows that the antioxidant stability of the facial mask is improved by adding the cosmetic emulsion containing the beta-glucosidase modified sodium alginate and the pumpkin seed extract into the facial mask.

Conventional operations in the operation steps of the present invention are well known to those skilled in the art and will not be described herein.

The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.

Claims

1. An anti-aging facial mask comprises humectant containing beta-glucosidase modified sodium alginate, propylene glycol, semen Cucurbitae extract, polyvinylpyrrolidone, arachidyl alcohol propionate, and water soluble ascorbic acid derivative;

the beta-glucosidase modified sodium alginate is prepared by solidifying beta-glucosidase on sodium alginate;

the humectant comprises, by weight, 0.4-5 parts of beta-glucosidase modified sodium alginate, 2-8 parts of propylene glycol, 0.5-3 parts of pumpkin seed extract, 0.02-2.5 parts of polyvinylpyrrolidone, 0.6-4.5 parts of arachidonol propionate and 0.01-5 parts of water-soluble ascorbic acid derivative;

the water-soluble ascorbic acid derivative is one or a mixture of L-sodium ascorbate, L-magnesium ascorbyl phosphate, L-calcium ascorbyl phosphate or L-sodium ascorbyl sulfate.

2. The anti-aging mask as set forth in claim 1, which is characterized in that: the anti-aging mask also comprises a thickening agent, liquid grease, a preservative and the balance of deionized water.

3. The method for making the anti-aging mask as claimed in claim 2, comprising the steps of:

s1: adding deionized water into a container, then adding the thickening agent and the liquid grease under the stirring action, homogenizing and stirring until the thickening agent and the liquid grease are completely dispersed, heating to 75-85 ℃, and then adding the humectant;

s2: cooling the components obtained in the step S1 to 30-40 ℃, adding a preservative and deionized water, and stirring and mixing uniformly;

s4: performing rotary evaporation on the component obtained in the step S3, and controlling the viscosity to obtain an emulsion for cosmetics;

s5: and adding the base cloth into the emulsion for cosmetics, soaking, taking out and carrying out vacuum packaging to obtain the facial mask.