CN112574864B - Stem cell culture dish mount - Google Patents
Stem cell culture dish mount Download PDFInfo
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- CN112574864B CN112574864B CN202011476303.2A CN202011476303A CN112574864B CN 112574864 B CN112574864 B CN 112574864B CN 202011476303 A CN202011476303 A CN 202011476303A CN 112574864 B CN112574864 B CN 112574864B
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- plate
- culture dish
- rod
- fixedly connected
- locating
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of culture dish fixing frame equipment, in particular to a stem cell culture dish fixing frame which comprises a fixing frame main body, wherein a positioning rod is fixedly connected to the front surface of the fixing frame main body, two ends of the positioning rod are movably connected with a limiting rod, the lower surface of the limiting rod is movably connected with a clamping plate through a shaft, the front surface of the limiting rod is movably connected with a crank connecting rod through a shaft, the upper ends of the crank connecting rods are movably connected with sliding blocks through shafts, and the force acting on the sliding blocks is mutually acting, so that the deformation force of a limiting spring reversely enables the clamping plate to squeeze a culture dish, thereby fixing the culture dish, avoiding workers touching the fixing frame to shake the culture dish, enabling a bactericide in an air cushion to be squeezed out, and performing sterilization treatment on the culture dish fixing frame, thereby avoiding bacterial infection caused by stem cells in the culture dish, and improving the culture efficiency of the stem cells.
Description
Technical Field
The invention relates to the technical field of culture dish fixing frame equipment, in particular to a stem cell culture dish fixing frame.
Background
Stem cells can be classified into embryonic stem cells and adult stem cells, and can be classified into totipotent stem cells, pluripotent stem cells, and multipotent stem cells according to different differentiation potential, which have characteristics of multidirectional differentiation potential, infinite division, proliferation, etc., and in the process of culturing stem cells, a culture vessel needs to be fixed, and a shelf needs to be used.
However, the existing shelf can not well stabilize the culture surface in the process of placing the culture surface, and staff can touch the shelf to shake the culture dish, so that the culture dish can be possibly influenced to cultivate stem cells, and sterilization treatment can not be well carried out on the periphery of the culture surface, so that bacteria infection is avoided, and the cultivation is influenced; accordingly, a stem cell culture dish holder is provided by those skilled in the art to solve the above-mentioned problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides a stem cell culture dish fixing frame, which is achieved by the following specific technical means:
the utility model provides a stem cell culture dish mount, includes the mount main part, the front surface fixedly connected with locating lever of mount main part, the equal swing joint in both ends of locating lever has the gag lever post, the lower surface of gag lever post is through axle swing joint has the grip block, the front surface of gag lever post is through axle swing joint has the crank connecting rod, the upper end of crank connecting rod is through axle swing joint has the slider, the right flank fixedly connected with stripper plate of slider, the right flank fixedly connected with limiting spring of stripper plate, the upper surface fixedly connected with movable plate of grip block, the lower surface of locating lever is located the outside fixedly connected with locating rack of movable plate, the inside fixedly connected with limiting plate of locating rack, the lower surface of limiting plate is located the top fixedly connected with gasbag of movable plate, the top swing joint of locating rack is located the limiting plate has the push rod, the upper end of push rod is through axle swing joint has the fly leaf, the right flank fixedly connected with reset spring of fly leaf, the left flank fixedly connected with reset spring of fly leaf, the inside fixedly connected with air cushion of guide plate.
As optimization, the inside of locating lever is offered and is mutually matched with the spout of slider.
As optimization, the limiting rods are distributed in bilateral symmetry with the vertical center line of the positioning rod.
As optimization, the limiting springs are distributed symmetrically left and right by the vertical center line of the positioning rod, and the right ends of the limiting springs are fixedly connected with the inner wall of the positioning rod.
As optimization, the inside of locating rack is offered and is mutually matched with the hole of movable plate.
As optimization, the limit plate is internally provided with a through hole.
As optimization, the sliding plates are distributed symmetrically left and right by the vertical center line of the return spring.
The invention has the following beneficial effects:
1. this stem cell culture dish mount through the stripper plate extrusion limit spring that makes on the slider right-hand member to make limit spring take place elastic deformation, because the effect is mutual, thereby make limit spring's deformation force reverse messenger clamp plate extrusion culture dish, thereby fix the culture dish, thereby avoid the staff to touch the mount and make the culture dish take place to rock, thereby avoid causing the influence to the stem cell in the culture dish, thereby improve the stability of culture dish.
2. This stem cell culture dish mount through the inner wall through-hole that makes the gas follow limiting plate enters into the below of piston board, the atmospheric pressure that makes the piston board below is greater than the sum of the restoring force of atmospheric pressure and spring of top, thereby make the piston board take place to remove at the inside of locating rack, thereby make the piston board drive the push rod and remove, thereby make the push rod drive the sliding plate of upper end remove, make the air cushion of the left side of deflector extrusion left side of sliding plate, thereby make the inside atmospheric pressure of air cushion change, thereby produce pressure differential with the external world, thereby make the inside germicide of air cushion squeeze out, thereby disinfect to the culture dish mount, thereby avoid the stem cell in the culture dish to cause the infection of bacterium, thereby can be fine provide the aseptic environment growth of stem cell, thereby the cultivation efficiency of stem cell that improves that can be fine.
Drawings
Fig. 1 is a schematic top view of a cross-sectional structure of a main body of a fixing frame according to the present invention.
FIG. 2 is a schematic view of a slider structure according to the present invention.
Fig. 3 is a schematic view of the structure of the stop lever of the present invention.
Fig. 4 is an enlarged view of area a in fig. 1.
Fig. 5 is an enlarged view of region B in fig. 3.
In the figure: 1. a fixing frame main body; 2. a positioning rod; 3. a limit rod; 4. a clamping plate; 5. a crank connecting rod; 6. a slide block; 7. an extrusion plate; 8. a limit spring; 9. a moving plate; 10. a positioning frame; 11. a limiting plate; 12. an air bag; 13. a piston plate; 14. a push rod; 15. a sliding plate; 16. a return spring; 17. a guide plate; 18. and (5) an air cushion.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-5, a stem cell culture dish fixing frame comprises a fixing frame main body 1, the front surface of the fixing frame main body 1 is fixedly connected with a locating rod 2, a chute matched with a sliding block 6 is formed in the inside of the locating rod 2, two ends of the locating rod 2 are movably connected with a limiting rod 3, the limiting rod 3 is symmetrically distributed in a left-right direction by a vertical central line of the locating rod 2, the lower surface of the limiting rod 3 is movably connected with a clamping plate 4 through a shaft, the front surface of the limiting rod 3 is movably connected with a crank connecting rod 5 through a shaft, the upper end of the crank connecting rod 5 is movably connected with a sliding block 6 through a shaft, the right side surface of the sliding block 6 is fixedly connected with a squeezing plate 7, the right side surface of the squeezing plate 7 is fixedly connected with a limiting spring 8, the limiting spring 8 is symmetrically distributed in a left-right direction by a vertical central line of the locating rod 2, the right end of the limiting spring 8 is fixedly connected with the inner wall of the locating rod 2, the upper surface of the clamping plate 4 is fixedly connected with a moving plate 9, a hole matched with the moving plate 9 is formed in the inside of the locating frame 10, the lower surface of the locating rod 2 is fixedly connected with a locating frame 10 through a shaft, the crank connecting rod 5 is movably connected with a sliding plate 6 through a shaft, the right side 11 is fixedly connected with an air bag 11, and an air bag 11 is fixedly connected with an air bag 11.
The inside of locating rack 10 is located the top swing joint of limiting plate 11 has piston plate 13, piston plate 13's upper surface has push rod 14 through axle swing joint, push rod 14's upper end has slide plate 15 through axle swing joint, slide plate 15 is bilateral symmetry distribution with the vertical central line of reset spring 16, slide plate 15's right flank fixedly connected with reset spring 16, slide plate 15's left surface fixedly connected with deflector 17, through the extrusion plate 7 extrusion limiting spring 8 that makes on the slider 6 right-hand member, thereby make limiting spring 8 take place elastic deformation, because the effect is mutual, thereby make the deformation force of limiting spring 8 reverse messenger clamp plate 4 extrusion culture dish, thereby fix the culture dish, thereby avoid the staff to touch the mount and make the culture dish take place to rock, thereby avoid causing the influence to the stem cell in the culture dish, thereby improve the stability of culture dish.
The inside of locating rack 10 is located the left side fixedly connected with air cushion 18 of deflector 17, through making the gas enter into the below of piston board 13 from the inner wall through-hole of limiting plate 11, make the atmospheric pressure below piston board 13 be greater than the sum of the restoring force of pneumatic and spring above, thereby make piston board 13 take place to remove in the inside of locating rack 10, thereby make piston board 13 drive push rod 14 and remove, thereby make push rod 14 drive slide plate 15 of upper end remove, make the deflector 17 extrusion left side of slide plate 15 air cushion 18, thereby make the atmospheric pressure in air cushion 18 change, thereby produce pressure differential with the external world, thereby make the inside germicide of air cushion 18 squeeze out, thereby disinfect the culture dish mount, thereby avoid the stem cell in the culture dish to cause the infection of bacterium, thereby can be fine provide the aseptic environment growth of stem cell, thereby the cultivation efficiency of stem cell can be fine improvement.
When in use, the culture dish is placed on the surface of the fixed frame main body 1, so that the culture dish is extruded to the clamping plate 4 in the placing process, the clamping plate 4 drives the moving plate 9 to move, the moving plate 9 moves in the positioning frame 10, meanwhile, the limiting rod 3 on the clamping plate 4 moves, when the moving plate 9 moves in the positioning frame 10, the moving plate 9 is extruded to the air bag 12 positioned above, the air pressure in the air bag 12 is changed, the air in the air bag 12 is extruded, the air enters the lower part of the piston plate 13 from the inner wall through hole of the limiting plate 11, the air pressure below the piston plate 13 is larger than the sum of the air pressure above and the restoring force of the spring, the piston plate 13 moves in the positioning frame 10, the piston plate 13 drives the push rod 14 to move, so that the push rod 14 drives the sliding plate 15 at the upper end to move, so that the sliding plate 15 pulls the return spring 16, so that the return spring 16 is elastically deformed, meanwhile, the guide plate 17 at the left side of the sliding plate 15 presses the air cushion 18 at the left side, so that the air pressure in the air cushion 18 is changed, and pressure difference is generated with the outside, so that the bactericide in the air cushion 18 is extruded, and the culture dish fixing frame is sterilized, so that the infection of bacteria caused by stem cells in the culture dish is avoided, so that the aseptic environment growth of the stem cells can be well provided, the culture efficiency of the stem cells can be well improved, meanwhile, in the process of the movement of the limit rod 3, the crank connecting rod 5 on the limit rod 3 moves along, so that the sliding block 6 at the upper end of the crank connecting rod 5 slides in the sliding groove in the positioning rod 2, thereby make the stripper plate 7 extrusion limit spring 8 on the slider 6 right-hand member to make limit spring 8 take place elastic deformation, because the effect is mutual, thereby make limit spring 8's deformation force reverse messenger clamp plate 4 extrusion culture dish, thereby fix the culture dish, thereby avoid the staff to touch the mount and make the culture dish take place to rock, thereby avoid causing the influence to the stem cell in the culture dish, thereby improve the stability of culture dish.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. The stem cell culture dish fixing frame comprises a fixing frame main body (1), and is characterized in that: the utility model discloses a piston rod, which is characterized in that a locating rod (2) is fixedly connected with the front surface of a fixing frame main body (1), a locating rod (3) is movably connected with the two ends of the locating rod (2), a clamping plate (4) is movably connected with the lower surface of the locating rod (3) through a shaft, a crank connecting rod (5) is movably connected with the front surface of the locating rod (3) through a shaft, a sliding block (6) is movably connected with the upper end of the crank connecting rod (5) through a shaft, a squeezing plate (7) is fixedly connected with the right side surface of the sliding block (6), a limit spring (8) is fixedly connected with the right side surface of the squeezing plate (7), a movable plate (9) is fixedly connected with the upper surface of the clamping plate (4), a locating frame (10) is fixedly connected with the lower surface of the locating rod (2) and is fixedly connected with a locating frame (10) through the outer part of the movable plate (9), a piston rod (12) is fixedly connected with the inner part of the locating frame (10), a piston plate (11) is fixedly connected with the upper part of the movable plate (9) through a shaft, a piston rod (14) is fixedly connected with the piston plate (12) inside the locating frame (10) and the upper surface of the locating frame (11) is positioned above the movable plate (9) and is movably connected with a push rod (14) through a sliding rod (13), the right side surface of the sliding plate (15) is fixedly connected with a reset spring (16), the left side surface of the sliding plate (15) is fixedly connected with a guide plate (17), and an air cushion (18) is fixedly connected to the left side of the guide plate (17) in the positioning frame (10);
a sliding groove matched with the sliding block (6) is formed in the positioning rod (2);
the limiting rods (3) are symmetrically distributed on the left and right with the vertical center line of the positioning rod (2);
the limiting springs (8) are symmetrically distributed on the left and right sides of the vertical center line of the positioning rod (2), and the right ends of the limiting springs (8) are fixedly connected with the inner wall of the positioning rod (2);
holes matched with the moving plate (9) are formed in the positioning frame (10);
the limiting plate (11) is internally provided with a through hole;
the sliding plates (15) are symmetrically distributed on the left and right by the vertical center line of the return spring (16);
when in use, the culture dish is placed on the surface of the fixed frame main body (1), thereby in the placing process, the culture dish is extruded to the clamping plate (4), thereby the clamping plate (4) drives the moving plate (9) to move, thereby the moving plate (9) moves in the positioning frame (10), meanwhile, the limiting rod (3) on the clamping plate (4) moves, when the moving plate (9) moves in the positioning frame (10), the moving plate (9) is extruded to the air bag (12) positioned above, thereby the air pressure in the air bag (12) is changed, thereby the air in the air bag (12) is extruded, thereby the air enters the lower part of the piston plate (13) from the inner wall through hole of the limiting plate (11), the air pressure below the piston plate (13) is larger than the sum of the restoring force of the air pressure above and the spring, thereby the piston plate (13) moves in the positioning frame (10), the piston plate (13) drives the push rod (14) to move, the push rod (14) moves, the push rod (14) drives the upper end (15) to move, and meanwhile, the air cushion (16) is extruded to the left side (16) is deformed, and the air cushion (18) is deformed, thereby the left side (16) is pushed to move, thereby produce pressure differential with the external world to make inside sterilant of air cushion (18) squeeze out, thereby disinfect to culture dish mount, thereby avoid stem cell in the culture dish to cause the infection of bacterium, thereby the aseptic environmental growth of providing stem cell that can be fine, thereby the cultivation efficiency of improvement stem cell that can be fine, simultaneously at the in-process of gag lever post (3) motion, thereby crank connecting rod (5) on messenger's gag lever post (3) follow the motion, thereby slider (6) at crank connecting rod (5) upper end slide in the spout of locating lever (2), thereby squeeze board (7) extrusion limiting spring (8) on messenger's slider (6) right-hand member, thereby make limiting spring (8) take place elastic deformation, because the effect is mutual, thereby make the deformation force reverse messenger clamping plate (4) extrusion culture dish of limiting spring (8), thereby fix the culture dish, thereby avoid the staff to touch the mount and make the culture dish take place to rock, thereby avoid causing the influence to the stem cell in the culture dish, thereby improve the stability of culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011476303.2A CN112574864B (en) | 2020-12-15 | 2020-12-15 | Stem cell culture dish mount |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011476303.2A CN112574864B (en) | 2020-12-15 | 2020-12-15 | Stem cell culture dish mount |
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| Publication Number | Publication Date |
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| CN112574864A CN112574864A (en) | 2021-03-30 |
| CN112574864B true CN112574864B (en) | 2023-08-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011476303.2A Active CN112574864B (en) | 2020-12-15 | 2020-12-15 | Stem cell culture dish mount |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113025494A (en) * | 2021-04-25 | 2021-06-25 | 河南亚都实业有限公司 | Artificial skin independent culture dish and protection structure thereof |
| CN113373046A (en) * | 2021-07-06 | 2021-09-10 | 济南惠凯诗生物科技有限公司 | Avoid glass to cultivate environment-friendly support auxiliary device that household utensils take place to burst |
| CN113579680A (en) * | 2021-08-18 | 2021-11-02 | 赵宴超 | High accuracy RV reduction gear automatic assembly device |
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| CN111986427A (en) * | 2020-08-28 | 2020-11-24 | 广州翼博慧互联网信息服务有限公司 | Forestry conflagration early warning device based on expend with heat and contract with cold principle |
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2020
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| CN103282782A (en) * | 2010-10-27 | 2013-09-04 | 株式会社百奥尼 | Automatic real-ime pcr system for the various analysis of biological smaple |
| CN106222085A (en) * | 2016-07-28 | 2016-12-14 | 西安交通大学 | A kind of high-precision biology is combined 3D printing equipment and Method of printing |
| CN207793249U (en) * | 2018-01-22 | 2018-08-31 | 刘玮清 | A kind of biological cell observation platform |
| CN208853403U (en) * | 2018-08-20 | 2019-05-14 | 云南爱尔发生物技术股份有限公司 | A kind of bioreactor cleaning device of systematization culture microalgae |
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Effective date of registration: 20230720 Address after: 056046 Building A9, Fukang Zhizao Future City, No. 7, Hexie Road, Jinan New District, Handan City, Hebei Province Applicant after: Handan Yilintang Pharmaceutical Chain Co.,Ltd. Address before: 201800 No. 2001, xupan Road, Jiading District, Shanghai Applicant before: Li Lou |
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