CN112569305A - Mushroom compound polysaccharide composition for improving immunity and preparation method thereof - Google Patents
Mushroom compound polysaccharide composition for improving immunity and preparation method thereof Download PDFInfo
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Abstract
The invention provides a lentinan composite polysaccharide composition for improving immunity and a preparation method thereof, wherein the lentinan composite polysaccharide composition comprises the following raw materials in parts by weight: 25-40 parts of mushroom extract, 8-16 parts of lucid ganoderma extract, 15-30 parts of cordyceps militaris extract, 25-40 parts of astragalus extract, 4-12 parts of codonopsis pilosula extract, 4-12 parts of garlic extract, 1-5 parts of bifidobacterium powder, 0.005-0.01 part of vitamin A, 0.0001-0.0005 part of vitamin E, 0.01-0.03 part of vitamin C, 0.01-0.02 part of ferric gluconate and 0.001-0.002 part of zinc gluconate. The lentinus edodes compound polysaccharide composition provided by the invention has a good effect of improving the immunity of an organism, and has the effects of protecting the liver, detoxifying, relieving fatigue, increasing beneficial microbial flora in intestinal tracts and improving the intestinal tract function.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine compositions, in particular to a lentinus edodes compound polysaccharide composition for improving immunity and a preparation method thereof.
Background
Immunity refers to the body's own ability to defend against diseases. It is the physiological response of identifying and eliminating invading foreign viruses, bacteria, fungi, etc., processing abnormal own cells, and identifying and eliminating "foreigners" by the human body. Low immunity means that the body's immune function is imbalanced or the immune system is not sound, and the body's defense capabilities are reduced. The "Emperor's Internal Path" records that "righteous qi is stored in the body, evil cannot be done, and where the evil is, the qi must be deficient." Righteous qi refers to the body's own immunity. Traditional Chinese medicine believes that "the insufficiency of righteous qi and the invasion of evil qi, and the imbalance of yin and yang of qi and blood" is the root cause of the weakened immune system. Insufficient righteousness leads to imbalance of yin and yang in the human body, dysfunction of the body, and susceptibility to external pathogen infections, lung deficiency causes constant colds, liver and kidney deficiency causes energy loss, spleen and stomach deficiency leads to decreased resistance, physical weakness, disease, and multiple symptoms recurring. Modern medical research has confirmed that active ingredients such as plant polysaccharides, mineral elements, vitamins, proteins, allicin, and Codonopsis saponins participate in human metabolism and play an important role in improving human immunity.
The Chinese patent publication No. CN107510059A discloses a preparation method and application of a polysaccharide composition, wherein the polysaccharide composition consists of black bean polysaccharide and oat polysaccharide, and the composition prepared by the invention has nutritive value, and has the functions of improving blood sugar regulation function and enhancing immunity.
However, the polysaccharide composition disclosed in the technical scheme has the defects of few polysaccharide source components and poor interaction between compound polysaccharides; the preparation process can not obtain high-purity polysaccharide, has the defects of single function and poor immunity enhancing function, and can not achieve the purpose of promoting immunity improvement by multiple purposes of one medicine.
Disclosure of Invention
The lentinus edodes compound polysaccharide composition has multiple polysaccharide source components, high polysaccharide purity and multi-path interaction, particularly strengthens the immune excitation effect of garlicin and improves the intestinal function, has good functions of enhancing immunity and multi-path metabolic regulation, and achieves the aim of multiple purposes of one medicine.
In order to achieve the purpose, the invention is realized by the following technical scheme:
the lentinan composite polysaccharide composition for improving the immunity comprises the following raw materials in parts by weight: 25-40 parts of mushroom extract, 8-16 parts of lucid ganoderma extract, 15-30 parts of cordyceps militaris extract, 25-40 parts of astragalus extract, 4-12 parts of codonopsis pilosula extract, 4-12 parts of garlic extract, 1-5 parts of bifidobacterium powder, 0.005-0.01 part of vitamin A, 0.0001-0.0005 part of vitamin E, 0.01-0.03 part of vitamin C, 0.01-0.02 part of ferric gluconate and 0.001-0.002 part of zinc gluconate.
Preferably, the lentinan composition comprises the following raw materials in parts by weight: 30-35 parts of mushroom extract, 10-15 parts of lucid ganoderma extract, 20-25 parts of cordyceps militaris extract, 30-35 parts of astragalus extract, 5-10 parts of codonopsis pilosula extract, 5-10 parts of garlic extract, 2-3 parts of bifidobacterium powder, 0.007-0.008 part of vitamin A, 0.0002-0.0003 part of vitamin E, 0.02-0.03 part of vitamin C, 0.015-0.02 part of ferric gluconate and 0.0015-0.002 part of zinc gluconate.
Preferably, the garlic extract is allicin.
The preparation method of the lentinus edodes compound polysaccharide composition for improving the immunity comprises the following steps:
(1) weighing 30-35 parts of shiitake mushroom, 10-15 parts of lucid ganoderma, 20-25 parts of cordyceps militaris and 30-35 parts of astragalus mongholicus according to parts by weight, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 8: 1, extracting for 1.5h under reflux, taking secondary filtrate, and combining the two filtrates;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; and adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing the ethanol to obtain the lentinan composite polysaccharide with the polysaccharide content of more than 30%.
(4) Weighing 5-10 parts of fresh garlic according to parts by weight, cutting into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis, recrystallizing at 15-25 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 5-10 parts of codonopsis pilosula according to parts by weight, cutting up the codonopsis pilosula, and then weighing the codonopsis pilosula in a mass ratio of M codonopsis pilosula: adding 95% ethanol into the mixture with the ratio of M ethanol to 1:6, extracting for 12h at the temperature of 40-50 ℃, filtering, washing filter residues with 95% ethanol for three times, recovering ethanol, and freeze-drying to obtain codonopsis pilosula ethanol extract freeze-dried powder;
(6) and (4) volatilizing ethanol from the codonopsis pilosula residue in the step (4), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with water at ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain radix Codonopsis water extract lyophilized powder, and mixing the water extract lyophilized powder with ethanol extract lyophilized powder to obtain radix Codonopsis extract;
(7) and (2) uniformly mixing the lentinus edodes compound polysaccharide extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances according to a formula ratio, and granulating to obtain the lentinus edodes compound polysaccharide composition.
Preferably, the pore size of the reverse osmosis concentration in the step (4) is 0.1-0.5 nm.
The lentinus edodes is a medicine-food homologous plant, has the effects of tonifying qi, not hunger, treating wind, breaking blood, benefiting stomach and assisting food, and is proved by modern medical research to contain a large amount of polysaccharide, protein, amino acid, multiple vitamins and the like, wherein the lentinus edodes polysaccharide is a key substance for improving the immunity, and the humoral immunity function of a human body is enhanced by improving the activity of auxiliary T cells. The folk has a common language of 'want to have good health and fungus soup is a treasure' from ancient times.
Ganoderma lucidum has been used in China for over 2000 years, and is regarded as a miraculous treasure by medical doctors of all ages for nourishing and strengthening body, strengthening body resistance and consolidating constitution. Ganoderma lucidum is rich in active substances such as polysaccharides, amino acids, polypeptides, proteins, triterpenes, alkaloids, vitamin B2, etc. The book Shen nong Ben Cao Jing (Shen nong's herbal classic) lists ganoderma as the top grade, and is called ' mainly deaf, benefit joint, keep spirit, benefit vital essence and energy, strengthen tendons and bones, good color, take for a long time to lose weight and prolong life '. A large number of clinical studies prove that the ganoderma lucidum has the effects of tonifying qi and blood, improving immunity, protecting liver, detoxifying, promoting utilization of tissues to sugar, improving myocardial microcirculation, enhancing myocardial oxygen and energy supply, reducing cholesterol, softening blood vessels, resisting oxidation, protecting skin and the like.
Vitamin A is very important for enhancing human immunity, can improve the reactivity of human cellular immunity and promote organisms to generate specific antibodies to pathogenic microorganisms such as bacteria, viruses and the like.
Vitamin E is an effective immunomodulator, and can promote development and cell differentiation of immune organs of organisms, and improve cellular immunity and humoral immunity of the organisms.
Vitamin C is an important immunomodulator, participates in the synthesis process of antibodies, promotes the generation of interferon in organisms, and improves the activity of white blood cells with phagocytic function.
The bifidobacterium can adjust the intestinal flora; synthesizing vitamins, promoting digestion of food and absorption of nutrient substances; the immunity of the organism is stimulated, the immune system activity is enhanced, macrophages can be activated, various important cytotoxic effector molecules are secreted in the period, and the invasion of external pathogenic bacteria is resisted.
The cordyceps militaris is also called as cordyceps militaris, is an artificial culture variety, has the same efficacy as the cordyceps militaris, and is an excellent substitute of the cordyceps militaris. The cordyceps sinensis wine is rich in cordycepin, cordycepic acid, superoxide dismutase, amino acid and various trace elements, has sweet taste and warm nature, has the effects of tonifying lung and kidney, replenishing essence and benefiting marrow, strengthening body resistance and tonifying qi, tonifying spleen, soothing nerves, improving immunity and the like, and is recorded in Chinese pharmacopoeia.
The astragalus is a tonifying medicinal material, and the ancient books record that the astragalus has the efficacies of strengthening body resistance and consolidating constitution and tonifying middle-jiao energy, and the modern medical research shows that the astragalus contains a large amount of astragalus polysaccharide and has the functions of tonifying spleen and Qi, improving kidney function and enhancing immunity.
The codonopsis pilosula is mainly used for treating the symptoms of fatigue deficiency, cough, dyspnea, chronic deficiency and all deficiency of qi, blood and body fluid, and the modern medical research shows that the codonopsis pilosula contains a large amount of polysaccharide, saponin, organic acid, amino acid and the like, and can improve the spleen and kidney functions and enhance the immunity of organisms.
The invention has the beneficial effects that:
(1) the lentinus edodes contains a large amount of polysaccharide, protein, amino acid, multiple vitamins and the like, wherein the lentinus edodes polysaccharide is a key substance for improving immunity and enhances the humoral immunity function of a human body by improving the activity of helper T cells. Ganoderma has effects in nourishing qi and blood, enhancing immunity, protecting liver, removing toxic substances, promoting utilization of sugar by tissue, improving myocardial microcirculation, enhancing myocardial oxygen and energy supply, reducing cholesterol, softening blood vessel, resisting oxidation, and protecting skin.
(2) The allicin is a better immune-stimulating Chinese herbal medicine, has obvious influence on the immune function of mice, increases the weight of spleen and thymus, increases the activation of T lymphocytes, increases the number of cells formed by spleen antibodies, improves the secretion level of monocytes and increases the release of lysozyme, so the allicin can improve the cellular immunity, the humoral immunity and the non-specific immune function. Allicin is combined with fat, sugar and protein in vivo to generate lipid allicin, saccharine allicin and composite protein, and the combination exerts the effect same as vitamin E, and has effects of resisting oxidation, improving insulin effect, and ensuring synthesis and secretion of insulin.
(3) The iron element is used as a trace element necessary for human bodies, has influence on the development of immune organs of the bodies, the formation of immune cells and the killing capacity of the immune cells, properly increases the absorption of the iron element and has important effect on the improvement of the immunity of the human bodies. The zinc element is used as a trace element necessary for human bodies, the lack of the zinc element has quick and obvious influence on the immune system, the related range is wide, and the functions of immune organs, cellular immunity, humoral immunity and the like are all reduced.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the lentinan composite polysaccharide composition for improving the immunity comprises the following raw materials in parts by weight: 25 parts of mushroom extract, 16 parts of lucid ganoderma extract, 15 parts of cordyceps militaris extract, 40 parts of astragalus extract, 4 parts of codonopsis pilosula extract, 12 parts of garlic extract, 1 part of bifidobacterium powder, 0.01 part of vitamin A, 0.0001 part of vitamin E, 0.03 part of vitamin C, 0.01 part of ferric gluconate and 0.002 part of zinc gluconate.
A preparation method of a lentinus edodes compound polysaccharide composition for improving immunity comprises the following steps:
(1) weighing the shiitake mushroom, the lucid ganoderma, the cordyceps militaris and the astragalus mongholicus according to the weight parts, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 1:8, extracting under reflux for 1.5h, taking secondary filtrate, combining the two filtrates, and mixing uniformly to obtain mixed extract;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing ethanol to obtain Lentinus Edodes compound polysaccharide with polysaccharide content of more than 30%;
(4) weighing 20 parts of fresh garlic according to parts by weight, cutting into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis with reverse osmosis concentration pore diameter of 0.1nm, recrystallizing at 25 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 5 parts of codonopsis pilosula according to parts by weight, cutting up the codonopsis pilosula, and then weighing the codonopsis pilosula in a mass ratio of M codonopsis pilosula: adding 95% ethanol into 1: 6M ethanol, extracting at 40 deg.C for 12 hr, filtering, washing the residue with 95% ethanol for three times, recovering ethanol, and lyophilizing to obtain radix Codonopsis ethanol extract lyophilized powder;
(6) and (4) volatilizing ethanol from the codonopsis pilosula residue in the step (4), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with M water at a ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain radix Codonopsis water extract lyophilized powder, and mixing radix Codonopsis water extract lyophilized powder with radix Codonopsis ethanol extract lyophilized powder to obtain radix Codonopsis extract lyophilized powder;
(7) and (2) according to the formula proportion, uniformly mixing the mixed extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances in proportion, and granulating to obtain the lentinan composition.
Example 2:
the lentinan composite polysaccharide composition for improving the immunity comprises the following raw materials in parts by weight: 40 parts of mushroom extract, 8 parts of ganoderma lucidum extract, 30 parts of cordyceps militaris extract, 25 parts of astragalus extract, 12 parts of codonopsis pilosula extract, 4 parts of garlic extract, 5 parts of bifidobacterium powder, 0.005 part of vitamin A, 0.0005 part of vitamin E, 0.01 part of vitamin C, 0.01 part of ferric gluconate and 0.001 part of zinc gluconate.
A preparation method of a lentinus edodes compound polysaccharide composition for improving immunity comprises the following steps:
(1) weighing the shiitake mushroom, the lucid ganoderma, the cordyceps militaris and the astragalus mongholicus according to the weight parts, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 8: 1, extracting for 1.5h under reflux, taking secondary filtrate, and combining the two filtrates;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing ethanol to obtain Lentinus Edodes compound polysaccharide with polysaccharide content of more than 30%;
(4) weighing 20 parts of fresh garlic according to parts by weight, cutting into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis with reverse osmosis concentration pore diameter of 0.1nm, recrystallizing at 25 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 15 parts of codonopsis pilosula according to parts by weight, cutting up the codonopsis pilosula, and then weighing the codonopsis pilosula in a mass ratio of M codonopsis pilosula: adding 95% ethanol into the mixture with the ratio of M ethanol to 1:6, extracting for 12h at the temperature of 40-50 ℃, filtering, washing filter residues with 95% ethanol for three times, recovering ethanol, and freeze-drying to obtain codonopsis pilosula ethanol extract freeze-dried powder;
(6) and (4) volatilizing ethanol from the codonopsis pilosula residue in the step (4), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with water at ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain lyophilized powder of water extract, and mixing lyophilized powder of radix Codonopsis with lyophilized powder of ethanol extract to obtain lyophilized powder of radix Codonopsis extract;
(7) and (2) according to the formula proportion, uniformly mixing the mixed extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances in proportion, and granulating to obtain the lentinan composition.
Example 3
The lentinan composite polysaccharide composition for improving the immunity comprises the following raw materials in parts by weight: 30 parts of mushroom extract, 10 parts of lucid ganoderma extract, 25 parts of cordyceps militaris extract, 35 parts of astragalus extract, 5 parts of codonopsis pilosula extract, 5 parts of garlic extract, 3 parts of bifidobacterium powder, 0.008 part of vitamin A, 0.0002 part of vitamin E, 0.02 part of vitamin C, 0.02 part of ferric gluconate and 0.002 part of zinc gluconate.
A preparation method of a lentinus edodes compound polysaccharide composition for improving immunity comprises the following steps:
(1) weighing the shiitake mushroom, the lucid ganoderma, the cordyceps militaris and the astragalus mongholicus according to the weight parts, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 8: 1, extracting for 1.5h under reflux, taking secondary filtrate, and combining the two filtrates;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing ethanol to obtain Lentinus Edodes compound polysaccharide with polysaccharide content of more than 30%;
(4) weighing 15 parts of fresh garlic according to parts by weight, cutting into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis with reverse osmosis concentration pore diameter of 0.1nm, recrystallizing at 25 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 15 parts of codonopsis pilosula according to parts by weight, cutting up the codonopsis pilosula, and then weighing the codonopsis pilosula in a mass ratio of M codonopsis pilosula: adding 95% ethanol into 1: 6M ethanol, extracting at 40 deg.C for 12 hr, filtering, washing the residue with 95% ethanol for three times, recovering ethanol, and lyophilizing to obtain radix Codonopsis ethanol extract lyophilized powder;
(6) and (4) volatilizing ethanol from the codonopsis pilosula residue in the step (4), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with M water at a ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain radix Codonopsis water extract lyophilized powder, and mixing radix Codonopsis water extract lyophilized powder with radix Codonopsis ethanol extract lyophilized powder to obtain radix Codonopsis extract lyophilized powder;
(7) and (2) according to the formula proportion, uniformly mixing the mixed extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances in proportion, and granulating to obtain the lentinan composition.
Example 4
The lentinan composite polysaccharide composition for improving the immunity comprises the following raw materials in parts by weight: 35 parts of mushroom extract, 15 parts of lucid ganoderma extract, 20 parts of cordyceps militaris extract, 30 parts of astragalus extract, 10 parts of codonopsis pilosula extract, 10 parts of garlic extract, 2 parts of bifidobacterium powder, 0.007 part of vitamin A, 0.0003 part of vitamin E, 0.03 part of vitamin C, 0.015 part of ferric gluconate and 0.002 part of zinc gluconate.
A preparation method of a lentinus edodes compound polysaccharide composition for improving immunity comprises the following steps:
(1) weighing the shiitake mushroom, the lucid ganoderma, the cordyceps militaris and the astragalus mongholicus according to the weight parts, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 1:8, extracting under reflux for 1.5h, taking secondary filtrate, combining the two filtrates, and mixing uniformly to obtain mixed extract;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing ethanol to obtain Lentinus Edodes compound polysaccharide with polysaccharide content of more than 30%;
(4) weighing 14 parts of fresh garlic according to parts by weight, cutting the fresh garlic into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis with reverse osmosis concentration pore diameter of 0.2nm, recrystallizing at 20 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 15 parts of codonopsis pilosula according to the weight parts, cutting up the codonopsis pilosula, and then weighing the codonopsis pilosula according to the mass ratio of M codonopsis pilosula: adding 95% ethanol into 1: 6M ethanol, extracting at 45 deg.C for 12h, filtering, washing the residue with 95% ethanol for three times, recovering ethanol, and freeze drying to obtain radix Codonopsis ethanol extract lyophilized powder;
(6) and (4) volatilizing ethanol from the codonopsis pilosula residue in the step (4), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with water at ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain radix Codonopsis water extract lyophilized powder, and mixing with ethanol extract lyophilized powder to obtain radix Codonopsis extract;
(7) and (2) according to the formula proportion, uniformly mixing the mixed extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances in proportion, and granulating to obtain the lentinan composition.
Typical cases
Case 1: in a case of male, 42 years old, hypodynamia, dizziness and amnesia, the product is taken 1 bag of the product obtained in the example 1 every day before lunch and dinner, the improvement starts after 15 days, the mental state is improved, the memory is enhanced, the immunity is improved, and the product is continuously taken for 1 month without side effect.
Case 2: when the product is taken 1 bag of the product obtained in example 2 every day before lunch and supper, the symptoms of soreness and skelalgia of waist disappear, the lung and kidney function returns to normal after 15 days, the influenza resistance is obviously enhanced, and the product is continuously taken for 1 month without side effect.
Case 3: in a certain season, women are 45 years old, tired, weak and have a faint head, 1 bag of the product obtained in the example 3 is taken every day before lunch and dinner, the product starts to improve in 7 days, and the product wakes up the next day without side effects such as somnolence, dizziness, drowsiness and the like, and is not addicted after being continuously taken for 1 month.
Case 4, wangzhi, male, 45 years old, dizziness, insomnia and dreamful sleep, the product was taken 1 bag of the product obtained in example 4 every day before lunch and dinner, the symptoms of dizziness began to improve after 7 days, the sleep quality at night was obviously improved, and the product was continuously taken for 1 month without addiction.
In case 5, any person with deficiency of both lung and kidney, soreness and pain of waist and legs, and susceptibility to common cold, the product was taken 1 bag of the drug in the reference CN107510059A each time before lunch and supper, and after 15 days, the disease was still not improved obviously and the resistance was low.
As can be seen from typical cases, the therapeutic effects of the lentinan compositions provided in examples 1-4 of the present invention are superior to those of the pharmaceutical composition provided in the prior art (reference CN 107510059A).
Experiment of
1. Experimental Material
Kunming mice, subject drug was the pharmaceutical composition prepared according to example 1. Shengmai oral liquid. Leukocyte diluent: prepared according to the proportion of glacial acetic acid 2ml distilled water 98ml and 1 percent methylene blue 2 drops before use.
2. Experimental methods and results
(1) Grouping and administration: kunming mice, male and female, each (15 + -2) g, were randomly divided into 8 groups of 10 mice each, namely normal group, high, medium and low dosage groups of tested drugs, pulse-activating oral liquid group, astragalus polysaccharide group and ganoderma polysaccharide group. The drug was dissolved or diluted with distilled water and each group of mice was gavaged at 0.5 ml/mouse/day. A test drug high dose group (1.20g/kg), a test drug medium dose group (0.60g/kg), a test drug low dose group (0.30g/kg), a ganoderma lucidum polysaccharide group (0.60g/kg), and an astragalus polysaccharide group (0.60 g/kg). The mixture is suspended by distilled water and then is intragastrically filled by 0.5 ml/tube. The dosage of the pulse-activating oral liquid is converted into 0.0026 of the dosage of adults; the normal group was also gavaged with distilled water at a rate of 0.5 ml/mouse. Animals were sacrificed 1 time daily for 7 consecutive days at 8 d. 20 μ L of blood was collected from the animal orbit and added to 380 μ L of leukocyte dilution, and leukocytes were counted under the microscope. Thymus and spleen of the mice were harvested, and after residual blood was blotted with filter paper, the weight (mg) was weighed and divided by the weight (g) of the mice, respectively, to obtain thymus index and spleen index.
The above indexes are calculated as mean and Standard Deviation (SD), statistical software SPSS11.0 is used for data analysis, and LSD method is used for comparison among groups, and the results are shown in Table 5.
(2) As a result: compared with the normal group, the pulse-activating oral liquid and each tested drug group have statistical significance on the influence of mouse peripheral leukocyte proliferation, and the astragalus polysaccharide group and the ganoderma polysaccharide group also have statistical significance on the influence of mouse peripheral leukocyte proliferation, but are weaker than the tested drug group (p is less than 0.05). Each dose group and pulse generation group of the tested medicaments can effectively inhibit the atrophy of the thymus of the mouse (p < 0.05); the tested drug dose groups and the pulse-activating oral liquid group have the effect of promoting the proliferation of the spleen of a mouse (p < 0.01). The experimental result shows that the tested medicine has the function of enhancing the immune function of mice, and the function is obviously stronger than that of the astragalus polysaccharide group and the ganoderma lucidum polysaccharide group.
TABLE 5 Effect of test drugs on peripheral leukocytes
| Group of | Peripheral white blood cell count |
| Normal group | 0.705±0.155×100 |
| High dose group of test drugs | 1.878±0.403×105** |
| Medium dose group of test drugs | 1.872±0.344×105** |
| Low dose group of test drugs | 1.857±0.341×105** |
| Astragalus polysaccharides group | 1.035±0.171×105* |
| Ganoderma lucidum polysaccharide group | 1.114±0.256×105* |
| Pulse-activating oral liquid | 1.1782±0.546×105** |
*P<0.05，**P<0.01 compared to the normal group.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (5)
1. The lentinan composite polysaccharide composition for improving the immunity is characterized by comprising the following raw materials in parts by weight: 25-40 parts of mushroom extract, 8-16 parts of lucid ganoderma extract, 15-30 parts of cordyceps militaris extract, 25-40 parts of astragalus extract, 4-12 parts of codonopsis pilosula extract, 4-12 parts of garlic extract, 1-5 parts of bifidobacterium powder, 0.005-0.01 part of vitamin A, 0.0001-0.0005 part of vitamin E, 0.01-0.03 part of vitamin C, 0.01-0.02 part of ferric gluconate and 0.001-0.002 part of zinc gluconate.
2. The lentinan composite polysaccharide composition for improving the immunity according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 30-35 parts of mushroom extract, 10-15 parts of lucid ganoderma extract, 20-25 parts of cordyceps militaris extract, 30-35 parts of astragalus extract, 5-10 parts of codonopsis pilosula extract, 5-10 parts of garlic extract, 2-3 parts of bifidobacterium powder, 0.007-0.008 part of vitamin A, 0.0002-0.0003 part of vitamin E, 0.02-0.03 part of vitamin C, 0.015-0.02 part of ferric gluconate and 0.0015-0.002 part of zinc gluconate.
3. The immunity enhancing lentinan composite composition of claim 2, wherein the garlic extract is allicin.
4. A method for preparing the lentinan complex composition for improving immunity according to any one of claims 1 to 3, which comprises the following steps:
(1) weighing 30-35 parts of shiitake mushroom, 10-15 parts of lucid ganoderma, 20-25 parts of cordyceps militaris and 30-35 parts of astragalus mongholicus according to parts by weight, crushing into 30-mesh coarse powder, and mixing to obtain mixture powder for later use;
(2) adding water into the mixture powder for extraction twice, wherein the mixture powder is extracted for the first time according to the mass ratio of M: adding water in a ratio of M water to 10, extracting for 2 hours under reflux, and taking primary filtrate; filtering residues for the second time according to the mass ratio M: adding water in a ratio of M water to 1:8, extracting under reflux for 1.5h, taking secondary filtrate, combining the two filtrates, and mixing uniformly to obtain mixed extract;
(3) concentrating and evaporating the extracting solution until the specific gravity d is 1.20, standing and cooling, wherein the ratio of the extract to the extract is M: m ethanol ═ 1:8, adding 80% ethanol, stirring, standing overnight, and centrifuging to obtain precipitate; and adding 2 times of 80% ethanol into the precipitate again, washing, drying under reduced pressure, and volatilizing the ethanol to obtain the lentinan composite polysaccharide with the polysaccharide content of more than 30%.
(4) Weighing 5-10 parts of fresh garlic according to parts by weight, cutting into small fragments, and mixing the fresh garlic with the small fragments according to a mass ratio of M garlic: adding 75% ethanol into 1:2, homogenizing with colloid mill for 30min, standing for 2 hr, centrifuging to separate Bulbus Allii tissue residue, concentrating the supernatant by reverse osmosis, recrystallizing at 15-25 deg.C to obtain fresh Bulbus Allii extract;
(5) weighing 5-10 parts of codonopsis pilosula according to parts by weight, chopping, and then mixing the codonopsis pilosula and ginseng according to a mass ratio of M: adding 95% ethanol into 1:6, extracting at 40-50 deg.C for 12h, filtering, washing the residue with 95% ethanol for three times, recovering ethanol, and freeze drying to obtain ethanol extract lyophilized powder;
(6) and (5) volatilizing ethanol from the codonopsis pilosula residue in the step (5), carrying out hot reflux water extraction, and mixing the medicine residues according to the mass M: extracting with water at ratio of 1:10 under reflux for 2 times, each time for 1 hr, mixing extractive solutions, concentrating under reduced pressure to relative density of 1.0-1.1, lyophilizing to obtain radix Codonopsis water extract lyophilized powder, and mixing the water extract lyophilized powder with ethanol extract lyophilized powder to obtain radix Codonopsis extract lyophilized powder;
(7) and (2) uniformly mixing the lentinan composite polysaccharide extract obtained in the step (1), the fresh garlic extract, the codonopsis pilosula extract and the rest other substances according to a formula ratio, and granulating to obtain the lentinan composition.
5. The method for preparing lentinan complex composition for improving immunity as claimed in claim 4, wherein the reverse osmosis concentration pore size in the step (4) is 0.1-0.5 nm.
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| CN114365844A (en) * | 2022-01-28 | 2022-04-19 | 福建农林大学 | Bamboo fungus compound health food for regulating intestinal flora and improving intestinal immunity and preparation method thereof |
| CN114558027A (en) * | 2022-03-15 | 2022-05-31 | 山东农业大学 | Preparation method and application of garlic polysaccharide-zinc compound |
| CN114698840A (en) * | 2022-04-06 | 2022-07-05 | 颖河(焦作)中药生物工程有限责任公司 | Natural plant extract for enhancing immunity and production process thereof |
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