CN112564675A - Method for detecting fluorescence signal - Google Patents
Method for detecting fluorescence signal Download PDFInfo
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- CN112564675A CN112564675A CN202011374366.7A CN202011374366A CN112564675A CN 112564675 A CN112564675 A CN 112564675A CN 202011374366 A CN202011374366 A CN 202011374366A CN 112564675 A CN112564675 A CN 112564675A
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- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000001514 detection method Methods 0.000 abstract description 18
- 230000001960 triggered effect Effects 0.000 description 9
- 239000000126 substance Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000004891 communication Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
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- H—ELECTRICITY
- H03—ELECTRONIC CIRCUITRY
- H03K—PULSE TECHNIQUE
- H03K5/00—Manipulating of pulses not covered by one of the other main groups of this subclass
- H03K5/125—Discriminating pulses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a method for detecting a fluorescence signal, which comprises the following steps: s1, determining a fluorescence signal and a reference voltage signal; s2, converting the fluorescence signal into a voltage signal; s3, amplifying the voltage signal; s4, judging whether the voltage signal is larger than the reference voltage signal by the comparator; if so, the comparator outputs a low level signal to enable the trigger to output a pulse signal; if not, the comparator outputs a high level signal, and the trigger does not output a pulse signal. Therefore, the invention realizes the detection of the fluorescence signal, has simple and practical method, reduces the difficulty of detecting the fluorescence signal and has stronger practicability.
Description
Technical Field
The invention relates to the technical field of detection, in particular to a method for detecting a fluorescence signal.
Background
The fluorescence detection technology is a common signal detection technology applied to various fields such as biology, analytical chemistry, geographic exploration, environmental monitoring and the like. The technology utilizes fluorescence induced by a certain substance (or excited substance) to reflect the characteristics of the substance, and qualitatively or quantitatively detects and analyzes the fluorescence, thereby determining the condition of the detected substance.
The existing fluorescent signal detection circuit mainly comprises a photodiode for acquiring a fluorescent signal. Because fluorescence induced by the excited detection substance is very weak, but the detection sensitivity of the photodiode is low, the photodiode acquires a fluorescence signal, the corresponding current signal obtained by conversion is in the fA-pA level, and the photodiode is difficult to effectively detect the weak fluorescence signal.
Therefore, how to realize effective detection of the fluorescence signal and improve the detection precision is an urgent problem to be solved by the technical personnel in the field.
Disclosure of Invention
The invention provides a method for detecting a fluorescence signal, which realizes the detection of the fluorescence signal, improves the accuracy, is simple, reduces the difficulty of detecting the fluorescence signal and has strong practicability.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
the embodiment of the invention provides a method for detecting a fluorescence signal, which comprises the following steps:
s1, determining a fluorescence signal and a reference voltage signal;
s2, converting the fluorescence signal into a voltage signal;
s3, amplifying the voltage signal;
s4, judging whether the voltage signal is larger than the reference voltage signal by the comparator;
if so, the comparator outputs a low level signal to enable the trigger to output a pulse signal;
if not, the comparator outputs a high level signal, and the trigger does not output a pulse signal.
Optionally, in an embodiment of the present invention, S4 further includes:
the trigger sends the pulse signals to a single chip microcomputer, and the single chip microcomputer counts the pulse signals and displays the pulse signals through an upper computer.
Optionally, in the embodiment of the present invention, the flip-flop is set to be triggered at a low level.
Optionally, in an embodiment of the present invention, S2 includes:
converting the fluorescence signal into a voltage signal by a converter.
Optionally, in an embodiment of the present invention, S3 includes:
and amplifying the voltage signal by an amplifier.
Optionally, in this embodiment of the present invention, the reference voltage signal is provided by chip LM336Z2.5.
Optionally, in the embodiment of the present invention, the model of the comparator is LM 360N.
Optionally, in the embodiment of the present invention, the type of the trigger is SN74LS 221N.
The invention has the advantages and positive effects that:
firstly, the fluorescent signal is very weak, so the amplification of the fluorescent signal can be realized through the amplifier, and the subsequent detection of the fluorescent signal is convenient.
Secondly, in the invention, the reference voltage signal and the amplified voltage signal can be compared through the comparator, specifically, when the amplified voltage signal is not greater than the reference voltage signal, the comparator outputs a high level signal, and does not trigger the trigger to output a pulse signal, that is, the amplified voltage signal can be regarded as noise, so that the interference is removed, and the detection precision is improved.
Third, in the invention, the upper computer and the single chip microcomputer are connected through the RS485 communication interface to realize data transmission, so that the upper computer can display the number of pulse signals, thereby realizing data uploading and downloading and being convenient for operators to record data.
Drawings
FIG. 1 is a flow chart of a method of detecting a fluorescent signal according to the present invention;
FIG. 2 is a block diagram of a converter and amplifier provided in accordance with the present invention;
FIG. 3 is a block diagram of a chip LM336Z2.5 provided in accordance with the present invention;
FIG. 4 is a block diagram of a comparator provided in accordance with the present invention;
fig. 5 is a block diagram of a flip-flop provided by the present invention.
Detailed Description
The description of the present invention referring to "first", "second", etc. is for descriptive purposes only, not specifically referring to the sequential or chronological meanings, but also not for limiting the present invention, and it is only for distinguishing the components or operations described in the same technical terms, and it is not to be understood as indicating or implying relative importance or implicitly indicating the number of the technical features indicated, whereby the features defined as "first", "second", etc. may explicitly or implicitly include at least one of the features.
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiments of the invention will be described in further detail below with reference to the accompanying drawings:
the method for detecting a fluorescence signal provided by the invention, as shown in fig. 1, comprises the following steps:
s1, determining a fluorescence signal and a reference voltage signal;
s2, converting the fluorescence signal into a voltage signal;
s3, amplifying the voltage signal;
s4, judging whether the voltage signal is larger than the reference voltage signal by the comparator;
if so, the comparator outputs a low level signal to enable the trigger to output a pulse signal;
if not, the comparator outputs a high level signal, and the trigger does not output a pulse signal.
Therefore, the fluorescent signal can be amplified through the amplifier, so that the fluorescent signal can be conveniently detected subsequently; the comparator is used for comparing the reference voltage signal with the amplified voltage signal, specifically, when the amplified voltage signal is not greater than the reference voltage signal, the comparator outputs a high-level signal and does not trigger the trigger to output a pulse signal, namely, the amplified voltage signal can be regarded as noise, so that interference is removed, and the detection precision is improved; the invention realizes the detection of the fluorescence signal, improves the accuracy, has simple method, reduces the difficulty of detecting the fluorescence signal and has stronger practicability.
Optionally, in an embodiment of the present invention, S4 further includes:
the trigger sends the pulse signals to a single chip microcomputer, and the single chip microcomputer counts the pulse signals and displays the pulse signals through an upper computer.
Wherein, the host computer is a PC, is an industrial computer.
And the computer and the singlechip are connected through an RS485 communication interface (or an RS232 communication interface) to realize data transmission.
So, count pulse signal through the singlechip to show through the host computer, also promptly, the number that the host computer can show pulse signal, thereby can realize data upload and download, the operating personnel record data of still being convenient for.
Optionally, in the embodiment of the present invention, the flip-flop is set to be triggered at a low level.
Wherein, the low level trigger can be understood as: when the low level signal output by the comparator is sent to the trigger, the trigger is triggered, so that the trigger outputs a pulse signal; when the high level signal output by the comparator is sent to the trigger, the trigger is not triggered, so that the trigger does not output the pulse signal.
Optionally, in an embodiment of the present invention, S2 includes:
converting the fluorescence signal into a voltage signal by a converter.
Since the fluorescence signal is a weak current signal, the fluorescence signal can be converted into a voltage signal by a converter, and specifically, as shown by a dotted line box in fig. 2, the fluorescence signal can be converted into a voltage signal by providing a resistor R1, wherein the resistance of R1 is 100 ohms.
Therefore, the voltage signal can be conveniently amplified subsequently, and the comparator can be used for comparing the amplified voltage signal with the reference voltage signal.
Optionally, in an embodiment of the present invention, S2 further includes:
and amplifying the voltage signal by an amplifier.
As shown IN fig. 2, a voltage signal is input to an IN + pin of the chip U1, and the input voltage signal can be amplified by an amplifier, so as to amplify the voltage signal and output the amplified voltage signal through an OUT-pin.
To explain this point, the purpose of amplifying the voltage signal is to: because the fluorescence signal is very weak, the amplification of the fluorescence signal can be realized through the amplifier, and the subsequent detection of the fluorescence signal is convenient.
Optionally, in this embodiment of the present invention, the reference voltage signal is provided by chip LM336Z2.5.
In the present invention, the reference voltage signal may be set to 2.5V, and certainly, is not limited to 2.5V, and may be set according to an actual requirement, and is not specifically limited herein.
As shown in fig. 3, the chip LM336Z2.5 provides a voltage signal, which is divided by the potentiometer W1 to obtain a reference voltage signal, and the reference voltage signal is sent to the comparator.
Optionally, in the embodiment of the present invention, the model of the comparator is LM 360N.
IN the present invention, as shown IN fig. 4, the voltage comparator receives an amplified voltage signal through an IN1 pin, the voltage comparator receives a reference voltage signal through an IN2 pin, and the voltage comparator compares the output reference voltage signal with the amplified voltage signal, so that the voltage comparator outputs different level signals.
Therefore, by arranging the comparator, the comparator compares the reference voltage signal with the amplified voltage signal, when the amplified voltage signal is not greater than the reference voltage signal, the comparator outputs a high-level signal and does not trigger the trigger to output a pulse signal, namely, the amplified voltage signal can be regarded as noise, so that interference is removed, and the detection precision is improved; the invention realizes the detection of the fluorescence signal, improves the accuracy, has simple method, reduces the difficulty of detecting the fluorescence signal and has stronger practicability.
Optionally, in the embodiment of the present invention, the type of the trigger is SN74LS 221N.
Thus, in the invention, a trigger with SN74LS221N model is arranged, a level signal sent by the comparator is received through the 1A pin, and when the trigger receives a low level signal, the trigger outputs a pulse signal; when the trigger receives a high level signal, the trigger does not output a pulse signal; in addition, the SN74LS221N type trigger has the characteristic of low power consumption, and the service life of the trigger can be prolonged to a certain extent.
The method for detecting a fluorescent signal will be described below with specific examples.
The fluorescent signal passes through a converter, the fluorescent signal is converted into a voltage signal, an amplifier amplifies the voltage signal and sends the amplified voltage signal to a comparator, the comparator compares the received amplified voltage signal with a reference voltage signal, wherein the reference voltage signal is provided by a chip LM336Z2.5, specifically, when the comparator judges that the amplified voltage signal is not greater than the reference voltage signal, the comparator outputs a high-level signal, and because the trigger is triggered by a low level, when the comparator sends the high-level signal to the trigger, the trigger is not triggered, that is, the trigger does not output a pulse signal; when the comparator judges that the amplified voltage signal is greater than the reference voltage signal, the comparator outputs a low level signal, and because the trigger is triggered by the low level, when the comparator sends the low level signal to the trigger, the trigger is triggered, namely, the trigger outputs a pulse signal and sends the output pulse signal to the single chip microcomputer, and the single chip microcomputer counts the pulse signal and displays the pulse signal through the upper computer.
Therefore, the invention realizes the detection of the fluorescent signal, judges that the amplified voltage signal is not greater than the reference voltage signal through the comparator, the trigger is not triggered, and the trigger does not output a pulse signal, namely, the voltage signal which does not meet the requirement is eliminated, in other words, the noise in the voltage signal is removed, thereby being beneficial to improving the detection precision.
It should be emphasized that the embodiments described herein are illustrative rather than restrictive, and thus the present invention is not limited to the embodiments described in the detailed description, but other embodiments derived from the technical solutions of the present invention by those skilled in the art are also within the scope of the present invention.
Claims (8)
1. A method for detecting a fluorescent signal, comprising:
s1, determining a fluorescence signal and a reference voltage signal;
s2, converting the fluorescence signal into a voltage signal;
s3, amplifying the voltage signal;
s4, judging whether the voltage signal is larger than the reference voltage signal by the comparator;
if so, the comparator outputs a low level signal to enable the trigger to output a pulse signal;
if not, the comparator outputs a high level signal, and the trigger does not output a pulse signal.
2. The method for detecting a fluorescent signal according to claim 1, wherein S4 further comprises:
the trigger sends the pulse signals to a single chip microcomputer, and the single chip microcomputer counts the pulse signals and displays the pulse signals through an upper computer.
3. A method of detecting a fluorescent signal as claimed in claim 1, wherein the trigger is set to a low level trigger.
4. The method for detecting a fluorescent signal according to claim 1, wherein S2 includes:
converting the fluorescence signal into a voltage signal by a converter.
5. The method for detecting a fluorescent signal according to claim 1, wherein S3 includes:
and amplifying the voltage signal by an amplifier.
6. The method of claim 1, wherein the reference voltage signal is provided by chip LM336Z2.5.
7. The method of claim 1, wherein the comparator is LM 360N.
8. The method of claim 1, wherein the trigger is model SN74LS 221N.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011374366.7A CN112564675A (en) | 2020-11-30 | 2020-11-30 | Method for detecting fluorescence signal |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011374366.7A CN112564675A (en) | 2020-11-30 | 2020-11-30 | Method for detecting fluorescence signal |
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| CN112564675A true CN112564675A (en) | 2021-03-26 |
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Citations (6)
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|---|---|---|---|---|
| US20060257153A1 (en) * | 2005-04-27 | 2006-11-16 | Seigo Furudate | Optical receiver applicable to multiple transmission speed |
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| CN1908740A (en) * | 2005-08-02 | 2007-02-07 | 凹凸科技（中国）有限公司 | Converter controller having feedforward compensation, converting system and method for controlling same |
| CN105093258A (en) * | 2015-09-30 | 2015-11-25 | 武汉中派科技有限责任公司 | Photon measurement front-end circuit |
| CN106850078A (en) * | 2017-03-30 | 2017-06-13 | 北京凌宇智控科技有限公司 | A kind of optical signal receiving circuit and optical signal receiving device |
| CN213426118U (en) * | 2020-11-30 | 2021-06-11 | 北京信息职业技术学院 | Detection circuit |
-
2020
- 2020-11-30 CN CN202011374366.7A patent/CN112564675A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070007906A1 (en) * | 2004-09-24 | 2007-01-11 | Ushio Denki Kabushiki Kaisha | Rare gas fluorescent lamp apparatus |
| US20060257153A1 (en) * | 2005-04-27 | 2006-11-16 | Seigo Furudate | Optical receiver applicable to multiple transmission speed |
| CN1908740A (en) * | 2005-08-02 | 2007-02-07 | 凹凸科技（中国）有限公司 | Converter controller having feedforward compensation, converting system and method for controlling same |
| CN105093258A (en) * | 2015-09-30 | 2015-11-25 | 武汉中派科技有限责任公司 | Photon measurement front-end circuit |
| CN106850078A (en) * | 2017-03-30 | 2017-06-13 | 北京凌宇智控科技有限公司 | A kind of optical signal receiving circuit and optical signal receiving device |
| CN213426118U (en) * | 2020-11-30 | 2021-06-11 | 北京信息职业技术学院 | Detection circuit |
Non-Patent Citations (1)
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| 张迒: "在线水中油测量***的设计", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》, no. 9, 15 September 2009 (2009-09-15), pages 13 - 14 * |
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