CN112545951A - Nano-liposome antioxidant membrane containing active plant extract - Google Patents

Nano-liposome antioxidant membrane containing active plant extract Download PDF

Info

Publication number
CN112545951A
CN112545951A CN201910908867.XA CN201910908867A CN112545951A CN 112545951 A CN112545951 A CN 112545951A CN 201910908867 A CN201910908867 A CN 201910908867A CN 112545951 A CN112545951 A CN 112545951A
Authority
CN
China
Prior art keywords
parts
active plant
nanoliposome
extract
chamomile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201910908867.XA
Other languages
Chinese (zh)
Inventor
丁小梅
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sinopoly Biotechnology (Guangdong) Group Co.,Ltd.
Original Assignee
Shenzhen Guangcai Life Engineering Technology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shenzhen Guangcai Life Engineering Technology Co ltd filed Critical Shenzhen Guangcai Life Engineering Technology Co ltd
Priority to CN201910908867.XA priority Critical patent/CN112545951A/en
Publication of CN112545951A publication Critical patent/CN112545951A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9794—Liliopsida [monocotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/0208—Tissues; Wipes; Patches
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/14—Liposomes; Vesicles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A61K8/553—Phospholipids, e.g. lecithin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9755—Gymnosperms [Coniferophyta]
    • A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/81—Preparation or application process involves irradiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/82—Preparation or application process involves sonication or ultrasonication

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Dermatology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Biomedical Technology (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

The invention discloses an antioxidant membrane containing active plant extract nanoliposomes, which takes grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol as raw materials, creatively prepares various active plant extracts into nanoliposome suspension, and embeds nutrients extracted from active plants in the nanoliposome form; due to the wrapping and detention effects of the bilayer structure of the nanoliposome on the active plant extract, the antioxidant membrane performance added with the nanoliposome is increased, and the active plant extract nanoliposome can be biodegraded, has stronger antioxidant stability, and also has a series of important health care effects of resisting aging, resisting cancer, resisting radiation, reducing blood fat, reducing blood sugar, reducing blood pressure, diminishing inflammation and the like.

Description

Nano-liposome antioxidant membrane containing active plant extract
Technical Field
The invention relates to the technical field of cosmetics, in particular to an antioxidant membrane containing active plant extract nanoliposomes.
Background
Among natural plant extracts, some plant extracts contain abundant antioxidant substances, so that the plant extracts have very strong free radical scavenging capacity and oil peroxidation prevention capacity; the nanoliposome is a bilayer vesicle with the particle size of 1-1000nm and has a structure similar to a biological membrane. The new form of liposomes as pharmaceutical formulations has the following characteristics: stability, sustained release, low toxicity, targeting, and variability. The plant extract can be made into particles with particle diameter of 1-1000nm by nanometer technology, and embedded in liposome. The active plant extract nanoliposome can be biodegraded in vivo due to the wrapping and retention effect of the bilayer structure of the nanoliposome on the plant extract, and has stronger oxidation resistance and stability.
Therefore, the plant extract can be used as an antioxidant to be added into the nano liposome, and then, the invention provides the nano liposome antioxidant membrane containing the active plant extract.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides an antioxidant membrane containing active plant extract nanoliposomes.
In order to achieve the purpose, the invention adopts the following technical scheme:
an active plant extract-containing nano liposome antioxidant membrane comprises the following raw materials in parts by weight: 4-8 parts of grape seeds, 6-10 parts of aloe leaves, 3-5 parts of chamomile, 3-5 parts of oligopeptide, 10-16 parts of pomegranate seeds, 1-2 parts of carrots, 1-2 parts of tomatoes, 1-2 parts of blueberries, 5-10 parts of pine barks, 1-2 parts of ginseng, 1-3 parts of fish gelatin, 5-10 parts of soybean lecithin, 10-20 parts of distilled water and 10-15 parts of glycerol.
Preferably, the pine bark is a red pine tree, the ginseng is American ginseng, the aloe is aloe barbadensis, the tomato is a red tomato, and the chamomile is german chamomile.
In addition, the invention also provides a preparation method of the nanometer liposome antioxidant membrane containing the active plant extract, which comprises the following steps:
s1: weighing grape seed, aloe leaf, chamomile, oligopeptide, pomegranate seed, carrot, tomato, blueberry, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol in proportion for later use;
s2: respectively preparing grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks and ginseng which are obtained in the step S1;
a: (1) parching grape seed, sieving with 30 mesh sieve, and squeezing oil to obtain grape seed oil; (2) cleaning folium Aloe, draining, ultraviolet sterilizing, peeling, adding folium Aloe into ultrasonic machine, ultrasonic crushing, filtering with gauze, freezing, and centrifuging; (3) cleaning chamomile, draining, sterilizing by ultraviolet rays, and putting the chamomile into an ultrasonic machine for treatment for 30 minutes to obtain chamomile essential oil; (4) washing, draining, squeezing and filtering the pomegranate seeds to obtain a pomegranate extracting solution; (5) washing carrot, draining, crushing, distilling and filtering to obtain carrot extract; (6) cleaning tomatoes, draining, drying, extracting with an organic solvent, and filtering filter residues to obtain a lycopene extract; (7) extracting blueberry with ethanol, filtering, recovering ethanol to obtain anthocyanin extract, adsorbing with AB-8 resin, eluting with 60% ethanol, recovering ethanol, extracting with petroleum ether, recovering petroleum ether, and drying to obtain anthocyanin extract; (8) drying pine bark at 40 ℃, crushing, sieving with a 40-mesh sieve, adding an enzyme solution for treatment, adding an ethanol solution, carrying out water bath oscillation extraction, and carrying out centrifugal extraction on supernatant to obtain pine polyphenol; (9) cleaning, air drying, crushing, heating and extracting ginseng for 2.5 hours at 85-90 ℃, continuously extracting for five times in the process, filtering, adjusting the pH value to 8-9 by using a 5% sodium hydroxide aqueous solution, standing and settling to obtain an extracting solution; (10) adding soybean lecithin into cholesterol to dissolve, and evaporating at 30-50 ℃ under reduced pressure to form a transparent film; b: adding the extract obtained in the step (1-9) in the step (a) into the extract obtained in the step (10) until the membrane is completely dissolved, and carrying out ultrasonic treatment at 100-150W for 5-30 minutes to obtain an active plant extract nanoliposome suspension;
s3: and (4) adding the gelatin, the distilled water, the glycerol and the oligopeptide obtained in the step S1 into the step b obtained in the step S2, and mixing to obtain the active plant extract nanoliposome suspension which is easy to absorb and has a good moisturizing effect.
S4: performing ultrasonic degassing on the extract in the step S3 to obtain a collagen membrane casting solution containing the active plant extract nanoliposomes, preserving heat at 30-45 ℃, and forming a membrane to obtain the active plant extract nanoliposome antioxidant membrane;
preferably, the extracts obtained in step S2 are separately filled, vacuum sealed and stored.
Preferably, in the step S4, 20mL of the casting solution is transferred to a 120mm × 80mm × 4mm organic glass plate at each time, and the film is formed by vacuum drying at 20-30 ℃ for 36-40 hours.
The invention provides an antioxidant membrane containing active plant extract nanoliposomes, which innovatively prepares various active plant extracts into nanoliposome suspension, and embeds nutrients extracted from active plants in the nanoliposome suspension by using the form of nanoliposomes; due to the wrapping and detention effects of the bilayer structure of the nanoliposome on the active plant extract, the antioxidant membrane performance added with the nanoliposome is increased, and the active plant extract nanoliposome can be biodegraded, has stronger antioxidant stability, and also has a series of important health care effects of resisting aging, resisting cancer, resisting radiation, reducing blood fat, reducing blood sugar, reducing blood pressure, diminishing inflammation and the like.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1
An active plant extract-containing nano liposome antioxidant membrane comprises the following raw materials in parts by weight: 4 parts of grape seeds, 6 parts of aloe leaves, 3 parts of chamomile, 3 parts of oligopeptide, 10 parts of pomegranate seeds, 1 part of carrot, 1 part of tomato, 1 part of blueberry, 5 parts of pine bark, 1 part of ginseng, 1 part of fish gelatin, 5 parts of soybean lecithin, 10 parts of distilled water and 10 parts of glycerol.
Specifically, the pine bark is a red pine tree, the ginseng is American ginseng, the aloe is aloe barbadensis, the tomato is a red tomato, and the chamomile is German chamomile.
In addition, the invention also provides a preparation method of the nanometer liposome antioxidant membrane containing the active plant extract, which comprises the following steps:
s1: weighing grape seed, aloe leaf, chamomile, oligopeptide, pomegranate seed, carrot, tomato, blueberry, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol in proportion for later use;
s2: respectively preparing grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks and ginseng which are obtained in the step S1;
(11) a: (1) parching grape seed, sieving with 30 mesh sieve, and squeezing oil to obtain grape seed oil; (2) cleaning folium Aloe, draining, ultraviolet sterilizing, peeling, adding folium Aloe into ultrasonic machine, ultrasonic crushing, filtering with gauze, freezing, and centrifuging; (3) cleaning chamomile, draining, sterilizing by ultraviolet rays, and putting the chamomile into an ultrasonic machine for treatment for 30 minutes to obtain chamomile essential oil; (4) washing, draining, squeezing and filtering the pomegranate seeds to obtain a pomegranate extracting solution; (5) washing carrot, draining, crushing, distilling and filtering to obtain carrot extract; (6) cleaning tomatoes, draining, drying, extracting with an organic solvent, and filtering filter residues to obtain a lycopene extract; (7) extracting blueberry with ethanol, filtering, recovering ethanol to obtain anthocyanin extract, adsorbing with AB-8 resin, eluting with 60% ethanol, recovering ethanol, extracting with petroleum ether, recovering petroleum ether, and drying to obtain anthocyanin extract; (8) drying pine bark at 40 ℃, crushing, sieving with a 40-mesh sieve, adding an enzyme solution for treatment, adding an ethanol solution, carrying out water bath oscillation leaching, and carrying out centrifugal extraction on a supernatant to obtain pine polyphenol, (9) cleaning ginseng, airing, crushing, heating and extracting with water at 85-90 ℃ for 2 hours, continuously extracting for five times in the above process, filtering, adjusting the pH value to 8-9 by using a 5% sodium hydroxide aqueous solution, and standing and settling to obtain an extracting solution; (10) adding soybean lecithin into cholesterol to dissolve, and evaporating at 30-50 ℃ under reduced pressure to form a transparent film; b: adding the extract obtained in the step (1-9) in the step (a) into the extract obtained in the step (10) until the membrane is completely dissolved, and carrying out ultrasonic treatment at 100-150W for 5-30 minutes to obtain an active plant extract nanoliposome suspension;
s3: and (4) adding the gelatin, the distilled water, the glycerol and the oligopeptide obtained in the step S1 into the step b obtained in the step S2, and mixing to obtain the active plant extract nanoliposome suspension which is easy to absorb and has a good moisturizing effect.
S4: performing ultrasonic degassing on the extract in the step S3 to obtain a collagen membrane casting solution containing the active plant extract nanoliposomes, preserving heat at 30-45 ℃, and forming a membrane to obtain the active plant extract nanoliposome antioxidant membrane;
specifically, the extracts obtained in the step S2 are respectively filled, vacuum sealed and stored.
Specifically, in the step S4, 20mL of casting solution is transferred to a 120mm × 80mm × 4mm organic glass plate every time, and the organic glass plate is vacuum-dried to form a film at the temperature of 20-30 ℃ for 36-40 hours.
Example 2
An active plant extract-containing nano liposome antioxidant membrane comprises the following raw materials in parts by weight: 6 parts of grape seeds, 8 parts of aloe leaves, 4 parts of chamomile, 4 parts of oligopeptide, 13 parts of pomegranate seeds, 1.5 parts of carrots, 1.5 parts of tomatoes, 1.5 parts of blueberries, 8 parts of pine barks, 1.5 parts of ginseng, 2 parts of fish gelatin, 8 parts of soybean lecithin, 15 parts of distilled water and 12 parts of glycerol.
Specifically, the pine bark is a red pine tree, the ginseng is American ginseng, the aloe is aloe barbadensis, the tomato is a red tomato, and the chamomile is German chamomile.
In addition, the invention also provides a preparation method of the nanometer liposome antioxidant membrane containing the active plant extract, which comprises the following steps:
s1: weighing grape seed, aloe leaf, chamomile, oligopeptide, pomegranate seed, carrot, tomato, blueberry, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol in proportion for later use;
s2: respectively preparing grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks and ginseng which are obtained in the step S1;
a: (1) parching grape seed, sieving with 30 mesh sieve, and squeezing oil to obtain grape seed oil; (2) cleaning folium Aloe, draining, ultraviolet sterilizing, peeling, adding folium Aloe into ultrasonic machine, ultrasonic crushing, filtering with gauze, freezing, and centrifuging; (3) cleaning chamomile, draining, sterilizing by ultraviolet rays, and putting the chamomile into an ultrasonic machine for treatment for 30 minutes to obtain chamomile essential oil; (4) washing, draining, squeezing and filtering the pomegranate seeds to obtain a pomegranate extracting solution; (5) washing carrot, draining, crushing, distilling and filtering to obtain carrot extract; (6) cleaning tomatoes, draining, drying, extracting with an organic solvent, and filtering filter residues to obtain a lycopene extract; (7) extracting blueberry with ethanol, filtering, recovering ethanol to obtain anthocyanin extract, adsorbing with AB-8 resin, eluting with 60% ethanol, recovering ethanol, extracting with petroleum ether, recovering petroleum ether, and drying to obtain anthocyanin extract; (8) drying pine bark at 40 ℃, crushing, sieving with a 40-mesh sieve, adding an enzyme solution for treatment, adding an ethanol solution, carrying out water bath oscillation extraction, and carrying out centrifugal extraction on supernatant to obtain pine polyphenol; (9) cleaning, air drying, crushing, heating and extracting ginseng for 2.5 hours at 85-90 ℃, continuously extracting for five times in the process, filtering, adjusting the pH value to 8-9 by using a 5% sodium hydroxide aqueous solution, standing and settling to obtain an extracting solution; (10) adding soybean lecithin into cholesterol to dissolve, and evaporating at 30-50 ℃ under reduced pressure to form a transparent film; b: adding the extract obtained in the step (1-9) in the step (a) into the extract obtained in the step (10) until the membrane is completely dissolved, and carrying out ultrasonic treatment at 100-150W for 5-30 minutes to obtain an active plant extract nanoliposome suspension;
s3: and (4) adding the gelatin, the distilled water, the glycerol and the oligopeptide obtained in the step S1 into the step b obtained in the step S2, and mixing to obtain the active plant extract nanoliposome suspension which is easy to absorb and has a good moisturizing effect.
S4: performing ultrasonic degassing on the extract in the step S3 to obtain a collagen membrane casting solution containing the active plant extract nanoliposomes, preserving heat at 30-45 ℃, and forming a membrane to obtain the active plant extract nanoliposome antioxidant membrane;
specifically, the extracts obtained in the step S2 are respectively filled, vacuum sealed and stored.
Specifically, in the step S4, 20mL of casting solution is transferred to a 120mm × 80mm × 4mm organic glass plate every time, and the organic glass plate is vacuum-dried to form a film at the temperature of 20-30 ℃ for 36-40 hours.
Example 3
An active plant extract-containing nano liposome antioxidant membrane comprises the following raw materials in parts by weight: 8 parts of grape seeds, 10 parts of aloe leaves, 5 parts of chamomile, 5 parts of oligopeptide, 16 parts of pomegranate seeds, 2 parts of carrots, 2 parts of tomatoes, 2 parts of blueberries, 10 parts of pine barks, 2 parts of ginseng, 3 parts of fish gelatin, 10 parts of soybean lecithin, 20 parts of distilled water and 15 parts of glycerol.
Specifically, the pine bark is a red pine tree, the ginseng is American ginseng, the aloe is aloe barbadensis, the tomato is a red tomato, and the chamomile is German chamomile.
In addition, the invention also provides a preparation method of the nanometer liposome antioxidant membrane containing the active plant extract, which comprises the following steps:
s1: weighing grape seed, aloe leaf, chamomile, oligopeptide, pomegranate seed, carrot, tomato, blueberry, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol in proportion for later use;
s2: respectively preparing grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks and ginseng which are obtained in the step S1;
a: (1) parching grape seed, sieving with 30 mesh sieve, and squeezing oil to obtain grape seed oil;
(12) cleaning folium Aloe, draining, ultraviolet sterilizing, peeling, adding folium Aloe into ultrasonic machine, ultrasonic crushing, filtering with gauze, freezing, and centrifuging;
(13) cleaning chamomile, draining, sterilizing by ultraviolet rays, and putting the chamomile into an ultrasonic machine for treatment for 30 minutes to obtain chamomile essential oil;
(14) washing, draining, squeezing and filtering the pomegranate seeds to obtain a pomegranate extracting solution;
(15) washing carrot, draining, crushing, distilling and filtering to obtain carrot extract;
(16) cleaning tomatoes, draining, drying, extracting with an organic solvent, and filtering filter residues to obtain a lycopene extract;
(17) extracting blueberry with ethanol, filtering, recovering ethanol to obtain anthocyanin extract, adsorbing with AB-8 resin, eluting with 60% ethanol, recovering ethanol, extracting with petroleum ether, recovering petroleum ether, and drying to obtain anthocyanin extract;
(18) drying pine bark at 40 deg.C, pulverizing, sieving with 40 mesh sieve, adding enzyme solution, adding ethanol solution, extracting in water bath under oscillating, centrifuging to obtain pine polyphenol,
(19) cleaning, air drying, crushing, heating and extracting ginseng for 2.5 hours at 85-90 ℃, continuously extracting for five times in the process, filtering, adjusting the pH value to 8-9 by using a 5% sodium hydroxide aqueous solution, standing and settling to obtain an extracting solution;
(20) adding soybean lecithin into cholesterol to dissolve, and evaporating at 30-50 ℃ under reduced pressure to form a transparent film;
b: adding the extract obtained in the step (1-9) in the step (a) into the extract obtained in the step (10) until the membrane is completely dissolved, and carrying out ultrasonic treatment at 100-150W for 5-30 minutes to obtain an active plant extract nanoliposome suspension;
s3: and (4) adding the gelatin, the distilled water, the glycerol and the oligopeptide obtained in the step S1 into the step b obtained in the step S2, and mixing to obtain the active plant extract nanoliposome suspension which is easy to absorb and has a good moisturizing effect.
S4: performing ultrasonic degassing on the extract in the step S3 to obtain a collagen membrane casting solution containing the active plant extract nanoliposomes, preserving heat at 30-45 ℃, and forming a membrane to obtain the active plant extract nanoliposome antioxidant membrane;
specifically, the extracts obtained in the step S2 are respectively filled, vacuum sealed and stored.
Specifically, in the step S4, 20mL of casting solution is transferred to a 120mm × 80mm × 4mm organic glass plate every time, and the organic glass plate is vacuum-dried to form a film at the temperature of 20-30 ℃ for 36-40 hours.
According to the antioxidant membrane containing the active plant extract nanoliposomes and the preparation method thereof, grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol are used as raw materials, the specific gravity of each component is strictly controlled, the traditional blending preparation formula is changed, the sources of the carrot, the tomato and the blueberry are wide, the raw material cost is saved, and the antioxidant membrane containing the active plant extract nanoliposomes has abundant carotene, lycopene and anthocyanin and is effective in antioxidation; the chamomile has the effects of calming and diminishing inflammation, the aloe leaves and the oligopeptides have the effect of moisturizing, the grape seeds and the pomegranate seeds have the effects of oxidation resistance and aging resistance, the pine bark has a good oxidation resistance effect, the ginseng has the effect of aging resistance, and the fish gelatin has higher amino acid and supplements collagen.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (5)

1. An antioxidant membrane containing active plant extract nanoliposomes is characterized in that: the raw materials are as follows by weight: 4-8 parts of grape seeds, 6-10 parts of aloe leaves, 3-5 parts of chamomile, 3-5 parts of oligopeptide, 10-16 parts of pomegranate seeds, 1-2 parts of carrots, 1-2 parts of tomatoes, 1-2 parts of blueberries, 5-10 parts of pine barks, 1-2 parts of ginseng, 1-3 parts of fish gelatin, 5-10 parts of soybean lecithin, 10-20 parts of distilled water and 10-15 parts of glycerol.
2. The nanoliposome antioxidant membrane containing active plant extracts as claimed in claim 1, wherein: the pine bark is red pine, the ginseng is American ginseng, the aloe is aloe barbadensis, the tomato is red tomato, and the chamomile is German chamomile.
3. The method for preparing the nanoliposome antioxidant membrane containing active plant extracts according to claim 1, wherein the method comprises the following steps: the method comprises the following steps:
s1: weighing grape seed, aloe leaf, chamomile, oligopeptide, pomegranate seed, carrot, tomato, blueberry, pine bark, ginseng, fish gelatin, soybean lecithin, distilled water and glycerol in proportion for later use;
s2: respectively preparing grape seeds, aloe leaves, chamomile, oligopeptides, pomegranate seeds, carrots, tomatoes, blueberries, pine barks and ginseng which are obtained in the step S1;
a: (1) parching grape seed, sieving with 30 mesh sieve, and squeezing oil to obtain grape seed oil;
(2) cleaning folium Aloe, draining, ultraviolet sterilizing, peeling, adding folium Aloe into ultrasonic machine, ultrasonic crushing, filtering with gauze, freezing, and centrifuging;
(3) cleaning chamomile, draining, sterilizing by ultraviolet rays, and putting the chamomile into an ultrasonic machine for treatment for 30 minutes to obtain chamomile essential oil;
(4) washing, draining, squeezing and filtering the pomegranate seeds to obtain a pomegranate extracting solution;
(5) washing carrot, draining, crushing, distilling and filtering to obtain carrot extract;
(6) cleaning tomatoes, draining, drying, extracting with an organic solvent, and filtering filter residues to obtain a lycopene extract;
(7) extracting blueberry with ethanol, filtering, recovering ethanol to obtain anthocyanin extract, adsorbing with AB-8 resin, eluting with 60% ethanol, recovering ethanol, extracting with petroleum ether, recovering petroleum ether, and drying to obtain anthocyanin extract;
(8) drying pine bark at 40 ℃, crushing, sieving with a 40-mesh sieve, adding an enzyme solution for treatment, adding an ethanol solution, carrying out water bath oscillation leaching, and carrying out centrifugal extraction on a supernatant to obtain pine polyphenol, (9) washing ginseng, airing, crushing, heating and extracting with water at 85-90 ℃ for 2.5 hours, continuously extracting for five times in the process, filtering, adjusting the pH value to 8-9 by using a 5% sodium hydroxide aqueous solution, and standing and settling to obtain an extracting solution;
(10) adding soybean lecithin into cholesterol to dissolve, and evaporating at 30-50 ℃ under reduced pressure to form a transparent film;
b: adding the extract obtained in the step (1-9) in the step (a) into the extract obtained in the step (10) until the membrane is completely dissolved, and carrying out ultrasonic treatment at 100-150W for 5-30 minutes to obtain an active plant extract nanoliposome suspension;
s3: and (4) adding the gelatin, the distilled water, the glycerol and the oligopeptide obtained in the step S1 into the step b obtained in the step S2, and mixing to obtain the active plant extract nanoliposome suspension which is easy to absorb and has a good moisturizing effect.
S4: and (4) performing ultrasonic degassing on the extract in the step (S3) to obtain a collagen membrane casting solution containing the active plant extract nanoliposomes, preserving heat at 30-45 ℃, and forming a membrane to obtain the active plant extract nanoliposome antioxidant membrane.
4. The method for preparing the nanoliposome antioxidant membrane containing active plant extracts as claimed in claim 3, wherein the method comprises the following steps: and respectively filling the extracts obtained in the step S2, and storing in a vacuum sealing manner.
5. The method for preparing the nanoliposome antioxidant membrane containing active plant extracts as claimed in claim 3, wherein the method comprises the following steps: and S4, 20mL of casting solution is transferred to a 120mm × 80mm × 4mm organic glass plate every time, and the organic glass plate is dried in vacuum to form a film at the temperature of 20-30 ℃ for 36-40 hours.
CN201910908867.XA 2019-09-25 2019-09-25 Nano-liposome antioxidant membrane containing active plant extract Pending CN112545951A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910908867.XA CN112545951A (en) 2019-09-25 2019-09-25 Nano-liposome antioxidant membrane containing active plant extract

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910908867.XA CN112545951A (en) 2019-09-25 2019-09-25 Nano-liposome antioxidant membrane containing active plant extract

Publications (1)

Publication Number Publication Date
CN112545951A true CN112545951A (en) 2021-03-26

Family

ID=75029139

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910908867.XA Pending CN112545951A (en) 2019-09-25 2019-09-25 Nano-liposome antioxidant membrane containing active plant extract

Country Status (1)

Country Link
CN (1) CN112545951A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114224837A (en) * 2021-11-09 2022-03-25 成都科建生物医药有限公司 Preparation method of antioxidant liposome

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102631381A (en) * 2012-04-18 2012-08-15 东北林业大学 Method for extracting polyphenol substance from Korean pine barks
CN103880806A (en) * 2014-04-14 2014-06-25 丹东君澳食品有限公司 Method for extracting anthocyanin from blueberry
CN104387779A (en) * 2014-11-05 2015-03-04 福州大学 Antioxidative collagen film containing active plant extract nanoliposome
CN105147577A (en) * 2015-08-17 2015-12-16 彭小兰 Toner capable of relieving melanin deposition and preparation method of toner
CN106389261A (en) * 2015-07-27 2017-02-15 李尧 Moisturizing cream

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102631381A (en) * 2012-04-18 2012-08-15 东北林业大学 Method for extracting polyphenol substance from Korean pine barks
CN103880806A (en) * 2014-04-14 2014-06-25 丹东君澳食品有限公司 Method for extracting anthocyanin from blueberry
CN104387779A (en) * 2014-11-05 2015-03-04 福州大学 Antioxidative collagen film containing active plant extract nanoliposome
CN106389261A (en) * 2015-07-27 2017-02-15 李尧 Moisturizing cream
CN105147577A (en) * 2015-08-17 2015-12-16 彭小兰 Toner capable of relieving melanin deposition and preparation method of toner

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
于菁: "《不可不知的自制天然面膜常识》", 30 April 2012 *
李宏等: "《保健食品安全与功能性评价》", 31 January 2019 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114224837A (en) * 2021-11-09 2022-03-25 成都科建生物医药有限公司 Preparation method of antioxidant liposome

Similar Documents

Publication Publication Date Title
CN102086185B (en) Oligomeric proanthocyanidins and method for extracting same
KR20050041259A (en) Use of a callus for development of natural cosmetics
KR101851623B1 (en) A cosmetic composition comprising spirulina extract for blue light interception
CN105524482A (en) Hylocereus polyrhizus flesh and pericarp red pigment and extraction method thereof
KR20120038586A (en) Cosmetic composition for improving skin senescence
US20090208544A1 (en) Method of obtaining phytoalexins
CN107308033A (en) A kind of preparation method of olive fruits high-purity polyphenol and its application in cosmetics
CN108125869A (en) Dried orange peel general flavone and its application in cosmetics are extracted from dried orange peel using ultrasonic wave auxiliary law
CN107362080B (en) A kind of extracting method of polyphenol in passion fruit pericarp
CN104223026A (en) Rose sauce and preparation method thereof
CN108721139A (en) A kind of moisture-keeping composition containing polysaccharide
CN105687035A (en) Compound ginkgo essential oil with effects of resisting oxidation and delaying skin aging
CN107213185A (en) A kind of blueberry leaf polyphenol preparation method with antioxidation activity
CN112545951A (en) Nano-liposome antioxidant membrane containing active plant extract
CN105859674A (en) Method for extracting dragon fruit peel anthocyanin
CN113244289A (en) Method for extracting camphor fruit anthocyanin
CN105533735A (en) Lyophilized Citrus reticulata powder capsule product
KR101981054B1 (en) Method for manufacturing capsule type soft products
KR20210056279A (en) Health Functional beverage composition, Health Functional beverage comprising same and preparing method of the same
KR20150132754A (en) A preparation method of an extract of Saliconia Herbacea L having antioxidant and antimicrobial activity
KR102253702B1 (en) Cosmetic composition comprising seaweed ingredients and seawater
KR101583595B1 (en) Cosmetic composition and a method of manufacturing
CN110577937A (en) SOD complex enzyme for removing freckles and beautifying skin and preparation method thereof
KR102410091B1 (en) Method for manufacturing of washes containing plant-derived natural extracts
KR20200084116A (en) Hibiscus syriacus (Althaea frutex) flower extraction method and food and cosmetic composition containing the Hibiscus syriacus flower extract for the health

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20220819

Address after: 13th Floor, Block A, Tellus Shuibei Jewelry Building, Northwest Side, No. 38, Shuibei 2nd Road, Cuijin Community, Cuizhu Street, Luohu District, Shenzhen, Guangdong 518000

Applicant after: Sinopoly Biotechnology (Guangdong) Group Co.,Ltd.

Address before: 518000 11th floor, No.2 workshop, Daqian industrial plant, 67 District, Xin'an street, Bao'an District, Shenzhen City, Guangdong Province

Applicant before: SHENZHEN GUANGCAI LIFE ENGINEERING TECHNOLOGY CO.,LTD.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20210326