CN112544543A - Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof - Google Patents
Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof Download PDFInfo
- Publication number
- CN112544543A CN112544543A CN202011411753.3A CN202011411753A CN112544543A CN 112544543 A CN112544543 A CN 112544543A CN 202011411753 A CN202011411753 A CN 202011411753A CN 112544543 A CN112544543 A CN 112544543A
- Authority
- CN
- China
- Prior art keywords
- regulation
- water
- barrel
- experiment
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000251468 Actinopterygii Species 0.000 title claims abstract description 42
- 230000020477 pH reduction Effects 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 190
- 238000002474 experimental method Methods 0.000 claims abstract description 81
- 230000029219 regulation of pH Effects 0.000 claims abstract description 81
- 238000001914 filtration Methods 0.000 claims abstract description 31
- 238000000746 purification Methods 0.000 claims abstract description 23
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 40
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 20
- 239000001569 carbon dioxide Substances 0.000 claims description 19
- 230000033228 biological regulation Effects 0.000 claims description 14
- 239000013535 sea water Substances 0.000 claims description 12
- 239000004576 sand Substances 0.000 claims description 10
- 230000001276 controlling effect Effects 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 6
- 229920000742 Cotton Polymers 0.000 claims description 4
- 239000010419 fine particle Substances 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000010979 pH adjustment Methods 0.000 claims description 2
- 230000009977 dual effect Effects 0.000 claims 9
- 238000011160 research Methods 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000035882 stress Effects 0.000 description 17
- 239000007789 gas Substances 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 230000000694 effects Effects 0.000 description 6
- 230000037326 chronic stress Effects 0.000 description 5
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 230000037328 acute stress Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 208000004434 Calcinosis Diseases 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 241000243321 Cnidaria Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008143 early embryonic development Effects 0.000 description 1
- 230000003670 easy-to-clean Effects 0.000 description 1
- 230000003090 exacerbative effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002842 otolith Effects 0.000 description 1
- 210000001265 otolithic membrane Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 238000009287 sand filtration Methods 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 230000012488 skeletal system development Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/047—Liquid pumps for aquaria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a circulating dual-regulation and control mode fish acidification stress experimental device and a use method thereof, the experimental device comprises a plurality of experimental barrels, a water body purification barrel and a filtering system, the water purification barrel comprises a filtering bin and a water storage bin, the bottoms of the experiment barrels are arranged to be conical, a drain outlet is formed in the bottom end of the experiment barrel, an escape-preventing net is arranged above the drain outlet, the water inlet end of the drainage pipe is connected with the drain outlets of the experiment barrels, the water outlet end is arranged above the filtering bin of the water purification barrel, so that water inside the experiment barrel can naturally flow into the water purification barrel, a filtering system is arranged in the filtering bin, a water pump is arranged in the water storage bin, the water outlet end of the water pump is connected with the pH regulation system, the two sets of pH regulation systems are connected in series, and the next-stage pH regulation system is connected with the experiment barrels through the water inlet pipeline. The invention is beneficial to ensuring the scientificity of the experimental method and the accuracy and reliability of the research result.
Description
Technical Field
The invention belongs to the technical field of fishery experiments, and particularly relates to a circulating dual-regulation and control mode fish acidification stress experiment device and a use method thereof.
Background
Ocean acidification is the phenomenon that the pH value is reduced due to the absorption of excessive carbon dioxide in the air by seawater, and the increase of the acidity of the seawater changes the chemical balance of the seawater, so that various marine organisms and even an ecosystem which depend on the stability of the chemical environment face huge threats. The heavy use of fuel-emitted CO2 by humans has resulted in a decrease in marine pH and is exacerbating the acidification as predicted by inter-government climate report expert committee assessment reports (IPCC for short). In recent years, much attention has been paid to the study of the effect of marine acidification on marine life, and the effect of marine acidification on the growth, development, reproduction, physiology and the like of marine life has been mainly focused. Research shows that marine acidification has adverse effect on life activities of calcifications such as coral, mollusk, crustacean, etc.; in addition, acidification of the ocean also has an effect on early embryonic and skeletal development in fish, and sensory organs such as otoliths in some fish are damaged and become behaviourally sluggish. The direct or indirect influence of ocean acidification on marine organisms is one of the hot spots of domestic and overseas research in recent years.
The marine fishes are important pillars of the marine culture industry in China, and although the marine fishes have certain acid-base regulation capacity, the marine acidification under the global warming condition can not only have potential influence on the growth, survival, development and metabolism of the fishes, but also can provide new challenges for the whole marine fish culture industry. At the present stage, aiming at the research on the influence of seawater acidification on fishes, the adjustment of the seawater pH value mainly depends on special equipment such as a carbon dioxide enriching device and the like, or is directly carried out by adopting a carbon dioxide filling mode. The problems existing in the method are mainly reflected in two aspects: firstly, the development of the experiment is limited by the purchase of special equipment; and secondly, the direct carbon dioxide filling mode can additionally cause stress reaction of the experimental fish in the water changing operation and the water body pH value irregular adjusting operation in the experimental process, so that the accuracy of the experimental result is influenced.
The research on the influence of acidification on fishes can be divided into acute stress and chronic stress according to the experimental period. In the acute stress experiment process, external factors except for the acidification factor need to be kept as stable as possible to effectively ensure the accuracy of the experiment result, and the extra water exchange or pH value adjustment operation is not beneficial to the development of the experiment; the experimental period of the chronic stress is relatively long and can be maintained for several months or even longer, and formally, in the experimental process of the chronic stress, the experimental fish needs to be fed normally, in order to reduce the extra operation stress to the maximum extent, the residual feed and fish feces in the experimental barrel need to be treated by a certain technical means, the stability of the pH value of the experimental water body in the whole experimental period is ensured to the maximum extent, and the error between parallel groups in an experimental treatment group is reduced, so that a circulating type dual-regulation and control mode fish acidification stress experimental device and method need to be developed, so that the series of problems are solved, and the scientificity of the experimental method and the accuracy of the data result are ensured.
Disclosure of Invention
The invention aims to provide a circulating dual-regulation and control mode fish acidification stress experimental device and a use method thereof, which ensure the stability of the pH value of a water body in an experimental period, avoid errors among parallel groups in the same treatment group, reduce additional operation stress in the experimental process, solve the problem of effectively cleaning residual feed and fish feces in the water body in a stress experiment, particularly in the long-term chronic stress experiment process, ensure the stability of other water quality factors except acidification factors in the experimental period, and ensure the scientificity of the experimental method and the accuracy and reliability of research results.
The invention provides a circulating dual-regulation mode fish acidification stress experiment device which comprises a plurality of experiment barrels, a water body purification barrel, a filtering system, two sets of pH regulation systems, a drainage pipeline and a water inlet pipeline, wherein the water body purification barrel comprises a filtering bin and a water storage bin, the bottoms of the experiment barrels are communicated with each other, the bottoms of the experiment barrels are arranged in a conical shape, a drain outlet is formed in the bottom end of the experiment barrel, an anti-escape net is arranged above the drain outlet, the water inlet end of the drainage pipeline is connected with the drain outlet of the experiment barrels, the water outlet end of the drainage pipeline is arranged above the filtering bin of the water body purification barrel, so that water in the experiment barrels can naturally flow into the water body purification barrel, the filtering bin is internally provided with the filtering system, the water storage bin is internally provided with a water pump, the water outlet end of the water pump is connected with the pH regulation systems, the two sets of pH regulation systems are connected in series, and the next-stage.
The pH regulation and control system comprises a pH regulation and control barrel, a carbon dioxide gas cylinder, a pH regulator, a pH sensor, an electromagnetic valve and an air disc, wherein the water outlet end of a water pump in the water storage bin is communicated with the pH regulation and control barrel, the pH sensor and the air disc are arranged in the pH regulation and control barrel, the air disc is connected with the electromagnetic valve of the carbon dioxide gas cylinder through an air pipe, and the pH regulator is respectively connected with the pH sensor and the electromagnetic valve and controls the electromagnetic valve through a pH signal detected by the pH sensor.
A water conveying pipe is arranged between the pH regulation barrels of the two sets of pH regulation systems, and the water inlet end of the water conveying pipe is connected with a water pump arranged in the upper-stage pH regulation barrel.
And a water pump is arranged in the next-stage pH regulation and control barrel, and the water inlet of the water inlet pipeline is connected with the water pump in the next-stage pH regulation and control barrel.
The water body in the pH regulation and control barrel forms a rotary flow state through water inlet position and angle control.
The filtering system sequentially comprises filtering cotton, fine particle sand, medium particle sand, large particle sand and corallite from top to bottom.
The bottom of the experiment barrel is supported by the supporting frame, so that the drain outlet of the experiment barrel is not lower than the upper port of the water body purification barrel.
And valves are arranged on branch pipes of the drainage pipeline and the water inlet pipeline which are respectively connected with the experiment barrels.
The technical scheme adopted by the invention for solving the technical problems is to provide a using method of a circulating dual-regulation and control mode fish acidification stress experimental device, which comprises the following steps:
(1) filling seawater into a pH regulation and control barrel connected with a water body purification barrel, enabling the water body in the pH regulation and control barrel to form rotary flow, setting the pH value required by an experiment through a pH regulator, controlling the opening of a solenoid valve by the pH regulator, opening a carbon dioxide gas cylinder to carry out acidification treatment on the water body, and controlling the closing of the solenoid valve to stop filling carbon dioxide when the pH value of the experimental water body reaches a set range;
(2) pumping the water body in the upper-stage pH regulation barrel after the pH value is regulated into the lower-stage pH regulation barrel, enabling the water body in the lower-stage pH regulation barrel to form rotary flow, and starting a pH regulator of a lower-stage pH regulation system to monitor and regulate the pH value of the water body in the pH regulation barrel in real time;
(3) filling the upper-stage pH regulation and control barrel with seawater again according to the step (1) and regulating the pH value of the water body;
(4) closing the drainage pipelines of the experimental barrels, and conveying water bodies for completing pH adjustment to the interior of the experimental barrels through the next-stage pH adjusting and controlling barrel;
(5) transferring half of the water body in the upper-stage pH regulation barrel into the lower-stage pH regulation barrel;
(6) by controlling the circulating flow frequency of the water body in the experimental device, after the operation is stable, the fish sample is put into a plurality of experimental buckets for experiment.
Advantageous effects
Firstly, the pH value of the water body is regulated in a double regulation mode in the pH regulation barrel, so that the stability of the pH value of the water body to be tested in an experiment period is ensured, the pH value of each experiment barrel does not need to be regulated, the stress reaction of the experimental fish caused by the regulation of the pH value in the experiment barrel is reduced to the maximum extent, the result difference between a plurality of experiment barrels (namely between parallel groups) caused by the asynchronous regulation of the pH value is eliminated, and the accuracy of the experiment result is improved.
Secondly, no equipment for inflation, pH detection and the like is arranged in the experiment barrel, the experiment barrel adopts high-level natural running water, and a water pump does not need to be additionally arranged in the barrel, so that the stress influence of additional operations (such as carbon dioxide filling for adjusting the pH value, water pumping by the water pump and the like) on the fishes in the experiment process is reduced to the maximum extent, and the accuracy of the experiment result is ensured.
Thirdly, the experiment barrel adopts the design that the bottom of the experiment barrel is provided with water and the bottom of the experiment barrel is conical, so that the problem of effectively cleaning the residual feed and the fish feces in the water body in the process of stress experiments, particularly long-term chronic stress experiments (needing feeding) is solved, daily pond washing and water changing operations of the experiment barrel are not needed, time and labor are saved, and correspondingly, the extra operation stress on the experimental fish is synchronously reduced.
Fourthly, the water body purification barrel is divided into a left bin and a right bin, the bottoms of the two bins are communicated, and the sand filtration system is arranged in the filtration bin, so that the stability of other water quality factors except the acidification factor in the experimental period can be ensured, and the scientificity of the acidification experimental method and the accuracy and reliability of the research result are ensured.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Detailed Description
The invention will be further illustrated with reference to the following specific examples. It should be understood that these examples are for illustrative purposes only and are not intended to limit the scope of the present invention. Further, it should be understood that various changes or modifications of the present invention may be made by those skilled in the art after reading the teaching of the present invention, and such equivalents may fall within the scope of the present invention as defined in the appended claims.
The circulating dual-regulation mode fish acidification stress experiment device shown in fig. 1 comprises an experiment barrel 1, a water body purification barrel 2, a filtering system 3, a pH regulation and control barrel 4, a carbon dioxide gas cylinder 5, a water pump 6, a pH regulator 7, a pH sensor 8, a pneumatic electromagnetic valve 9, a nanometer gas disc 10, a water pipe valve 11, a drainage pipeline 12, a water inlet pipeline 13 and an escape-proof net 14.
The bottom of the experimental barrel 1 is conical and discharges sewage in the center, an anti-escape net 14 is arranged above a sewage discharge outlet at the bottom, and the bottom water outlets of the three experimental barrels 1 are connected to a drainage pipeline 12 through pipelines. The water outlet of the drainage pipeline 12 is arranged above the water body purifying barrel 2. The inside of the water body purifying barrel 2 is divided into a left bin and a right bin, the left side is a filtering bin, the right side is a water storage bin, and the bottoms of the two bins are communicated. A set of filtering system 3 is arranged in the filtering of the water body purifying barrel 2 corresponding to the water outlet of the drainage pipeline 12. A water pump 6 is placed in another water storage bin in the water purification barrel 2, the water outlet of the water pump 6 is arranged above the pH regulation and control barrel 4, a set of pH regulation and control device 7 is arranged in the pH regulation and control barrel 4, the tail end of the pH regulation and control device 7 is connected with a pH sensor 8, the pH regulation and control device 7 is connected with a carbon dioxide gas cylinder 5 through a pneumatic electromagnetic valve 9, and the other end of the pneumatic electromagnetic valve 9 is connected with a nanometer gas disk 10. When the pH value of the water body reaches the upper limit of the pH value set by the experiment, the pH controller 7 transmits a signal to the pneumatic electromagnetic valve 9, and then carbon dioxide is released toward the water body, when the lower limit of the pH value set by the experiment is reached, the pneumatic electromagnetic valve 9 is controlled to be closed through the pH controller 7, a water pump 6 is placed in the pH regulation and control barrel 4, the water outlet of the water pump 6 is arranged at the upper position in another pH regulation and control barrel 4, the pH regulation and control barrel 4 is also provided with a pH regulator 7, a pH sensor 8, a pneumatic electromagnetic valve 9, a nanometer gas disk 10 and a carbon dioxide gas cylinder 5, and the water outlet of the water pump 6 in the pH regulation and control barrel 4 is connected to a water inlet pipeline 13 above the.
The water body purification barrel 2 is internally divided into a left bin and a right bin, the bottoms of the left bin and the right bin are communicated, and a set of filtering system 3 is arranged in the made filtering bin to block residual feed, fish feces and other granular substances.
The filtering system 3 is a set of small sand filtering system, and sequentially comprises filtering cotton, fine particle sand, medium particle sand, large particle sand and corallite from top to bottom. The purpose of the filtering system 3 is to maintain the stability of other water quality parameters of the water body except for acidification factors during the experiment, and the filtering cotton placed on the uppermost layer has the beneficial effects of being capable of isolating particulate matters and being easy to clean.
The water outlet of the water pump in the water purifying barrel 2 is arranged above the pH adjusting barrel 4, and the water outlet direction and the barrel wall form an angle of about 30 degrees, so that the water in the pH adjusting barrel 4 is always in a rotating flow state, and the pH value of the whole water body is ensured to be uniform in the process of adjusting the pH value of the water body.
The pH regulation and control bucket 4 is provided with 2 sets, and the volume of the effective water body is more than 3 times of the effective water body volume of experiment bucket 1 in single pH regulation and control bucket 4, and two sets of pH regulation and control buckets 4 pass through water pump 6 and connect, and the water pump 6 export is upper in second level pH regulation and control bucket 4 equally, and goes out the water direction and be the angle about 30 degrees with the bucket wall, and the purpose is the rotatory mobile state of guaranteeing the bucket internal water body always equally, makes the pH value of whole water even unanimity. The two sets of pH regulation and control barrels 4 have the advantages that the stability of the pH value of the experimental water body is ensured, the frequent floating of the pH value of the water body during the experiment is avoided, in addition, the pH value is uniformly regulated in the pH regulation and control barrels 4, the pH value regulation in each experiment barrel 1 is not needed, the additional operation stress on the experimental fish is reduced, the deviation of the pH value of the water body in 3 experiment barrels 1 is also avoided, and the error between parallel groups in the same treatment group is eliminated.
The water inlet pipe 13 is arranged above the experiment barrel 1, the water inlet end is connected with the water pump 6 in the second-stage pH regulation and control barrel 4, the circulating flow of the experiment water body in the whole system is realized by controlling the water inlet pipe valve 11, the circulating frequency of the water body in the experiment barrel is controlled once every 2-3 hours, and the dissolved oxygen content of the water body in the experiment barrel can be ensured to meet the requirement of the experiment fish.
The use method of the circulating dual-regulation and control mode fish acidification stress experimental device comprises the following steps:
1. the seawater is filled in a pH regulation and control barrel 4 connected with a water purification barrel 2, the pH value required by the experiment is set through a pH regulation and control device 7, the pH regulation and control device 7 transmits a signal to a pneumatic electromagnetic valve 9, the carbon dioxide is opened to carry out the acidification treatment of the water body, a water pump in the barrel is opened during the period, but a water outlet needs to be placed in the barrel firstly, the purpose is to enable the water body in the barrel to rotate and flow, the uniformity of the acidification treatment of the water body in the barrel is realized, when the pH value of the experiment water body reaches a set range, the pH regulation and control device transmits a signal to close the pneumatic electromagnetic valve, and the carbon dioxide charging is.
2. The water body in the pH regulation and control barrel 4 with the well-adjusted pH value is transferred into another pH regulation and control barrel 4 through a water pump 6, in the transfer process, the water outlet of the water pump and the barrel wall form an angle of about 30 degrees, so that the water body rotates and flows, a pH regulation and control device 7 in another pH regulation and control barrel 4 is started, and the pH value of the water body in the barrel is monitored and regulated in real time.
3. And filling the pumped pH regulation barrel 4 with seawater, and regulating the pH value of the water body according to the steps.
4. When the two pH regulation and control barrels 4 are filled with seawater and the pH value of the water body is regulated to the requirement of an experiment, the water inlet pipeline above the experiment barrel 1 is connected with the water pump in the pH regulation and control barrel 4, the water pump is started, the experiment water body is uniformly distributed into 3 experiment barrels, and at the moment, the water pipe valve at the bottom of the experiment barrel is closed.
5. And transferring half of the water body in the pH regulation and control barrel connected with the water body purification barrel into the other pH regulation and control barrel again through the water pump.
6. And finally, controlling the circulating flow frequency of the water body in the whole system through a water pump and a water pipe valve, and putting the experimental fish into an experimental barrel to start an experiment after the operation is stable and fixed.
Claims (9)
1. The utility model provides a circulating dual regulation and control mode fish acidizing threatens experimental apparatus, includes a plurality of experiment buckets (1), water purification bucket (2), filtration system (3), two sets of pH regulation and control systems, drainage pipe (12) and inlet channel (13), its characterized in that: the water purification barrel (2) comprises a filtering bin and a water storage bin which are communicated with each other at the bottoms, the bottoms of a plurality of experiment barrels (1) are arranged to be conical, the bottom of the experiment barrel (1) is provided with a drain outlet and an anti-escape net (14) above the drain outlet, the water inlet end of a drainage pipeline (12) is connected with the drain outlet of the experiment barrels (1), the water outlet end of the drainage pipeline is arranged above the filtering bin of the water purification barrel (2) to enable the water inside the experiment barrel (1) to naturally flow into the water purification barrel (2), a filtering system (3) is arranged in the filtering bin, a water pump (6) is arranged in the water storage bin, the water outlet end of the water pump (6) is connected with a pH regulation system, the two sets of pH regulation systems are connected in series with each other, and the next-level pH regulation system is connected with the experiment barrels (1).
2. The circulating dual regulation and control mode fish acidification stress experimental device of claim 1, characterized in that: the pH regulation and control system comprises a pH regulation and control barrel (4), a carbon dioxide gas cylinder (5), a pH regulator (7), a pH sensor (8), an electromagnetic valve (9) and a gas disc (10), wherein a water outlet end of a water pump (6) in the water storage bin is communicated with the pH regulation and control barrel (4), the pH sensor (8) and the gas disc (10) are arranged in the pH regulation and control barrel (4), the gas disc (10) is connected with the electromagnetic valve (8) of the carbon dioxide gas cylinder (5) through a gas pipe, and the pH regulator (7) is respectively connected with the pH sensor (8) and the electromagnetic valve (9) and controls the electromagnetic valve (9) through a pH signal detected by the pH sensor (8).
3. The circulating dual regulation and control mode fish acidification stress experimental device of claim 2, characterized in that: a water delivery pipe is arranged between the pH regulation barrels (4) of the two sets of pH regulation systems, and the water inlet end of the water delivery pipe is connected with a water pump (6) arranged in the upper-stage pH regulation barrel (4).
4. The circulating dual regulation and control mode fish acidification stress experimental device of claim 2, characterized in that: a water pump (6) is arranged in the next-stage pH regulation and control barrel (4), and the water inlet of the water inlet pipeline (13) is connected with the water pump (6) in the next-stage pH regulation and control barrel (4).
5. The circulating dual regulation and control mode fish acidification stress experimental device of claim 2, characterized in that: the water body in the pH regulation and control barrel (4) forms a rotary flowing state through water inlet position and angle control.
6. The circulating dual regulation and control mode fish acidification stress experimental device of claim 1, characterized in that: the filtering system (3) sequentially comprises filtering cotton, fine particle sand, medium particle sand, large particle sand and corallite from top to bottom.
7. The circulating dual regulation and control mode fish acidification stress experimental device of claim 1, characterized in that: the bottom of the experiment barrel (1) is supported by the supporting frame, so that the drain outlet of the experiment barrel (1) is not lower than the upper port of the water body purification barrel (2).
8. The circulating dual regulation and control mode fish acidification stress experimental device of claim 1, characterized in that: and valves (11) are arranged on branch pipes of the drainage pipeline (12) and the water inlet pipeline (13) which are respectively connected with the experiment barrels (1).
9. The use method of the circulating dual regulation and control mode fish acidification stress experimental device of claim 2, comprising the following steps:
(1) filling seawater into a pH regulation and control barrel (4) connected with a water body purification barrel (2) and enabling the water body in the pH regulation and control barrel (4) to form rotary flow, setting a pH value required by an experiment through a pH regulator (7), opening a solenoid valve (9) by the pH regulator (7), opening a carbon dioxide gas cylinder (5) to carry out acidification treatment on the water body, and stopping filling carbon dioxide when the pH value of the experiment water body reaches a set range and controlling the solenoid valve (9) to be closed by the pH regulator (7);
(2) pumping and transferring the water body in the upper-stage pH regulation and control barrel (4) with the adjusted pH value into the lower-stage pH regulation and control barrel (4), enabling the water body in the lower-stage pH regulation and control barrel (4) to form rotary flow, and starting a pH regulator (7) of a lower-stage pH regulation and control system to monitor and adjust the pH value of the water body in the pH regulation and control barrel (4) in real time;
(3) filling the upper-stage pH regulation and control barrel (4) with seawater again according to the step (1) and regulating the pH value of the water body;
(4) the drainage pipelines (12) of the experimental barrels (1) are closed, and water bodies for completing pH adjustment are conveyed to the inner parts of the experimental barrels (1) through the next-stage pH adjusting and controlling barrel (4);
(5) transferring half of the water body in the upper-stage pH regulation barrel (4) into the lower-stage pH regulation barrel (4);
(6) by controlling the circulating flow frequency of the water body in the experimental device, after the operation is stable, the implemented fish sample is placed in the experimental barrels (1) for experiment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011411753.3A CN112544543A (en) | 2020-12-03 | 2020-12-03 | Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011411753.3A CN112544543A (en) | 2020-12-03 | 2020-12-03 | Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112544543A true CN112544543A (en) | 2021-03-26 |
Family
ID=75048750
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011411753.3A Pending CN112544543A (en) | 2020-12-03 | 2020-12-03 | Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112544543A (en) |
Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318578A (en) * | 2011-08-02 | 2012-01-18 | 复旦大学 | Pipeline system for industrial cultivation of Trachidermus fasciatus |
| CN202346814U (en) * | 2011-10-26 | 2012-07-25 | 中国石化江汉油田分公司盐化工总厂 | Automatic pH value control device for light saline water |
| CN104663560A (en) * | 2015-01-31 | 2015-06-03 | 宁波大学 | Laboratory simulation ocean acidification system |
| CN104898725A (en) * | 2015-06-18 | 2015-09-09 | 国家***第三海洋研究所 | Seawater acidification control system for simulating future ocean acidification |
| CN105439271A (en) * | 2014-09-01 | 2016-03-30 | 中国石油化工股份有限公司 | Method and apparatus for continuously adjusting pH value |
| CN205124749U (en) * | 2015-12-01 | 2016-04-06 | 陈惠平 | Black turtle culture pond |
| CN205233161U (en) * | 2015-12-10 | 2016-05-18 | 宁波大学 | Indoor aquatic products biological incubation test device |
| BG2690U1 (en) * | 2017-06-21 | 2017-08-31 | Йордан Стефанов Стайков | Installation for integrated breeding of hydrobionts and plants in closed cycle |
| CN207284797U (en) * | 2017-09-09 | 2018-05-01 | 甘肃万马泉农业生态科技有限责任公司 | A kind of trout fry rearing system |
| CN207375806U (en) * | 2017-10-09 | 2018-05-18 | 杭州青化社化工有限公司 | Cyanide wastewater circularly purifying processing system |
| CN207512014U (en) * | 2017-08-22 | 2018-06-19 | 陕西赫星节能环保科技有限公司 | A kind of passivating wastewater processing unit |
| CN207918631U (en) * | 2017-12-30 | 2018-09-28 | 广东省石油与精细化工研究院 | A kind of on-line monitoring aquiculture sewerage processing experimental system |
| CN109298142A (en) * | 2018-11-30 | 2019-02-01 | 中国水产科学研究院黄海水产研究所 | Open-air enclosure water flow system capable of automatically controlling pH value and experimental method |
-
2020
- 2020-12-03 CN CN202011411753.3A patent/CN112544543A/en active Pending
Patent Citations (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318578A (en) * | 2011-08-02 | 2012-01-18 | 复旦大学 | Pipeline system for industrial cultivation of Trachidermus fasciatus |
| CN202346814U (en) * | 2011-10-26 | 2012-07-25 | 中国石化江汉油田分公司盐化工总厂 | Automatic pH value control device for light saline water |
| CN105439271A (en) * | 2014-09-01 | 2016-03-30 | 中国石油化工股份有限公司 | Method and apparatus for continuously adjusting pH value |
| CN104663560A (en) * | 2015-01-31 | 2015-06-03 | 宁波大学 | Laboratory simulation ocean acidification system |
| CN104898725A (en) * | 2015-06-18 | 2015-09-09 | 国家***第三海洋研究所 | Seawater acidification control system for simulating future ocean acidification |
| CN205124749U (en) * | 2015-12-01 | 2016-04-06 | 陈惠平 | Black turtle culture pond |
| CN205233161U (en) * | 2015-12-10 | 2016-05-18 | 宁波大学 | Indoor aquatic products biological incubation test device |
| BG2690U1 (en) * | 2017-06-21 | 2017-08-31 | Йордан Стефанов Стайков | Installation for integrated breeding of hydrobionts and plants in closed cycle |
| CN207512014U (en) * | 2017-08-22 | 2018-06-19 | 陕西赫星节能环保科技有限公司 | A kind of passivating wastewater processing unit |
| CN207284797U (en) * | 2017-09-09 | 2018-05-01 | 甘肃万马泉农业生态科技有限责任公司 | A kind of trout fry rearing system |
| CN207375806U (en) * | 2017-10-09 | 2018-05-18 | 杭州青化社化工有限公司 | Cyanide wastewater circularly purifying processing system |
| CN207918631U (en) * | 2017-12-30 | 2018-09-28 | 广东省石油与精细化工研究院 | A kind of on-line monitoring aquiculture sewerage processing experimental system |
| CN109298142A (en) * | 2018-11-30 | 2019-02-01 | 中国水产科学研究院黄海水产研究所 | Open-air enclosure water flow system capable of automatically controlling pH value and experimental method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Summerfelt | Design and management of conventional fluidized-sand biofilters | |
| HRP20240273T1 (en) | Multi-phasic modularised shrimp production system and method | |
| CN102037922B (en) | Treatment method for industrial aquiculture circulating water | |
| CN104285864A (en) | High-density circulating water stepwise shrimp cultivation system and transfer method thereof | |
| CN106342742A (en) | Temporary culture system of marine fish by using circulating water | |
| CN104719119B (en) | A kind of application method for being used to expand the equipment of phytoplankton | |
| CN206641209U (en) | A kind of intensive crab cultivation device | |
| WO2012005667A1 (en) | System for biological fermentation | |
| CN106386636B (en) | Integral culture method and system capable of circularly filtering pond water | |
| CN112544543A (en) | Circulating dual-regulation and control mode fish acidification stress experimental device and use method thereof | |
| CN201008306Y (en) | Water circulating system for vast scale fish jar culture | |
| CN208327533U (en) | The filter device of ferrimanganic in a kind of removal underground water | |
| CN203546020U (en) | Algae high-density culture apparatus | |
| TWM648899U (en) | Exosome manufacturing system | |
| CN206118839U (en) | System is supported temporarily to sea water fish circulating water | |
| CN106520527B (en) | A kind of device and method generated using microorganism induction guanite | |
| US20060118484A1 (en) | Process and device for biological treatment of a suspension in a bioreactor | |
| CN111073794B (en) | Gas-driven microalgae culture algae liquid circulating device and use method | |
| CN208500609U (en) | A kind of life afloat sewage purifying and treating device | |
| CN206118851U (en) | Sea water fish supports pond water filtering ponds temporarily | |
| CN207918631U (en) | A kind of on-line monitoring aquiculture sewerage processing experimental system | |
| CN204191381U (en) | A kind of high density shrimps cycle water staged cultivating system | |
| CN206562427U (en) | Immobilized microalgae cultivates culture medium feedway and cultivating system | |
| CN218007749U (en) | Closed circulating water culture experimental system for aquatic organism hypoxia stress | |
| CN103571743B (en) | High-density alga culture device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210326 |