CN112538501A - 腺相关病毒因子viii载体 - Google Patents

腺相关病毒因子viii载体 Download PDF

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CN112538501A
CN112538501A CN202011117582.3A CN202011117582A CN112538501A CN 112538501 A CN112538501 A CN 112538501A CN 202011117582 A CN202011117582 A CN 202011117582A CN 112538501 A CN112538501 A CN 112538501A
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P·C·克罗希
A·纳斯瓦尼
J·麦金托什
E·塔德纳姆
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St Jude Childrens Research Hospital
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Abstract

本发明提供了改进的腺相关病毒(AAV)因子VIII(FVIII)载体，包括产生功能性因子VIII多肽的AAV FVIII载体以及具有高表达活性的AAV FVIII载体。

Description

腺相关病毒因子VIII载体
本申请要求2013年9月12日提交的美国临时专利申请序列号61/877,042的优先权，该美国临时专利申请以引用的方式整体并入本文。
技术领域
本发明涉及腺相关病毒(AAV)因子VIII(FVIII)载体，包括具有高表达活性的AAVFVIII载体和表达全长或截短型功能性FVIII的AAV FVIII载体。本发明还涉及制备本文所述的AAV FVIII载体的方法和其相关的治疗用途。
背景技术
腺相关病毒(AAV)是一种感染人类和一些其它灵长类物种的小的有复制缺陷的无包膜的动物病毒。AAV的若干特征使得这种病毒成为用于通过基因治疗递送治疗性蛋白质的有吸引力的运载体，包括例如已知AAV不会引起人类疾病并且AAV诱导轻微的免疫反应，以及AAV载体可以感染***的细胞和休眠细胞这两者而不整合到宿主细胞基因组中。使用AAV的基因治疗载体已经被成功地用于一些临床试验中，例如用于向肝脏递送人类因子IX(FIX)以治疗B型血友病(Nathwani等,New Engl.J.Med.365:2357-2365,2011)。
然而，AAV基因治疗载体确实有一些缺点。具体来说，AAV载体的克隆能力由于所述病毒的DNA包装能力而是有限的。野生型AAV的单链DNA基因组是约4.7个千碱基(kb)。实际上，最多约5.0kb的AAV基因组似乎被完全包装(即全长)到AAV病毒颗粒中。对于AAV载体中的核酸基因组必须具有两个具有约145个碱基的AAV反向末端重复序列(ITR)的要求来说，AAV载体的DNA包装能力使得蛋白质编码序列的最多约4.4kb可以被衣壳化。
由于这一尺寸限制，大的治疗性基因，即长度大于约4.4kb的那些一般不适用于AAV载体中。一种这样的治疗性基因是因子VIII(FVIII)基因，它具有编码具有2332个氨基酸的多肽的约7.0kb的mRNA，所述多肽从N末端到C末端包含具有19个氨基酸的信号肽以及三个大的结构域(即重链或A结构域、中央或B结构域、以及轻链或C结构域)。已经被用于针对FVIII克服AAV载体尺寸限制的一种策略在于使用两种AAV载体，一种编码重链或A结构域，并且另一种编码轻链或C结构域(参见例如Coutu等的美国专利号6,221349、6,200,560以及7,351,577)。规避这一尺寸限制的另一种策略在于产生编码如下的FVIII的AAV载体，其中所述蛋白质的中央部分或B结构域已经缺失并且被具有14个氨基酸的接头置换，所述接头被称为SQ序列(Ward等,Blood,117:798-807,2011；以及McIntosh等,Blood 121:3335-3344,2013)。
虽然在文献中已经报道AAV载体具有>5.0kb的AAV基因组，但是在这些情况中的许多情况下，所编码的基因的5'末端或3'末端似乎被截短(参见Hirsch等,Molec.Ther.18-6-8,2010；以及Ghosh等,Biotech.Genet.Engin.Rev.24:165-178,2007)。然而，已经证实的是，在受AAV感染的细胞中在具有5'末端截短的核酸与具有3'末端截短的核酸之间存在重叠的同源重组，以使得产生编码大蛋白质的“完整”核酸，从而重新构建功能性的全长基因。
对可用于基因治疗方法中以治疗A型血友病的编码功能性因子VIII蛋白质的新型AAV载体存在需要。因而，本发明涉及AAV载体，所述AAV载体编码功能活性FVIII以使得AAV病毒体将编码治疗性蛋白质的整个核酸衣壳化，即完全包装的AAV FVIII载体，从而避免尺寸过大的基因组的上述问题，或至少产生可能被截短或可能没有被截短的功能活性因子VIII蛋白质。此外，为了避免衣壳引导的免疫反应，AAV载体应当具有每个衣壳颗粒的靶蛋白的最高可能的转导/表达活性。本发明还涉及具有高表达活性的完全AAV FVIII载体的制备。最终，本发明涉及用于产生本文所述的AAV因子VIII载体的方法以及使用其的相关方法。
发明内容
本发明提供了编码功能活性FVIII的AAV载体(在本文被称为“AAV FVIII载体”)。编码功能活性FVIII的基因组优选地具有最多7.0kb的长度，更优选地具有最多6.5kb的长度，还更优选地具有最多6.0kb的长度，还更优选地具有最多5.5kb的长度，还更优选地具有最多5.0kb的长度，具有增强的启动子功能。
如本文所用的“功能活性FVIII”是当在培养细胞中表达时具有野生型FVIII蛋白质的体外功能或当在细胞或身体组织中表达时具有野生型FVIII蛋白质的体内功能的FVIII蛋白质。这包括例如在患有A型血友病的受试者中允许发生凝血以及缩短血液凝固所耗费的时间。野生型FVIII经由凝血级联，充当激活的FIX(FIXa)的辅因子来参与凝血，所述激活的FIX在钙离子和磷脂存在下形成复合体，所述复合体将因子X(FX)转化成激活的FX(FXa)。因此，功能活性FVIII可以与FIXa形成复合体，所述复合体可以将FX转化成FXa。
如本文所用的“AAV载体”指的是单链或双链的核酸，所述核酸具有：侧接蛋白质编码序列的AAV 5'反向末端重复(ITR)序列和AAV 3'ITR，所述蛋白质编码序列与转录调节元件，即一个或多个启动子和/或增强子、以及多聚腺苷酸化序列可操作地连接；以及任选的***到所述蛋白质编码序列的外显子之间的一个或多个内含子。单链AAV载体指的是存在于AAV病毒颗粒的基因组中的核酸，并且所述核酸可以是本文所公开的核酸序列的正义链或反义链。这样的单链核酸的尺寸是以碱基提供的。双链AAV载体指的是存在于用于表达或转移AAV载体核酸的质粒(例如pUC19)的DNA或双链病毒(例如杆状病毒)的基因组中的核酸。这样的双链核酸的尺寸是以碱基对(bp)提供的。
如本文所用的术语“反向末端重复序列(ITR)”指的是存在于AAV基因组的5'末端和3'末端处的本领域公认的区域，所述区域以顺式充当DNA复制起点和病毒基因组的包装信号。AAV ITR连同AAV rep编码区一起提供在两个侧接的ITR之间***到宿主细胞基因组中的核苷酸序列的高效切除和拯救以及整合。某些AAV相关ITR的序列由Yan等,J.Virol.79(1):364-379(2005)公开，该文献以引用的方式整体并入本文。
“转录调节元件”指的是涉及调节基因转录的基因的核苷酸序列，包括启动子、加上响应元件、用于结合转录因子以有助于RNA聚合酶结合并且促进表达的激活子和增强子序列、以及与阻遏蛋白结合以阻断RNA聚合酶连接并且阻止表达的操纵子或沉默子序列。术语“肝脏特异性转录调节元件”指的是特异性地调节肝脏组织中的基因表达的调节元件。肝脏特异性调节元件的实例包括但不限于小鼠甲状腺素运载蛋白启动子(mTTR)、内源性人类因子VIII启动子(F8)、人类α-1-抗胰蛋白酶启动子(hAAT)和其活性片段、人白蛋白最小启动子、以及小鼠白蛋白启动子。还涵盖了源自于肝脏特异性转录因子结合位点的增强子，如EBP、DBP、HNF1、HNF3、HNF4、HNF6以及Enh1。
在一个实施方案中，本发明的AAV载体包含一种核酸，所述核酸编码具有被具有14个氨基酸的SQ序列置换的B结构域的功能活性FVIII，即编码FVIII SQ。所述SQ序列公开在Ward等,Blood,117:798-807,2011；以及McIntosh等,Blood 121:3335-3344,2013中。所述FVIII编码区序列是密码子优化的序列(参见Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1，该美国专利申请公开以引用的方式整体并入本文；以及McIntosh等,Blood 121:3335-3344,2013)。这个序列在本文被称作“UCL SQ FVIII”。
在第一个方面，本发明的AAV载体包含Proto 1，所述Proto 1示意性地描绘于图2A中并且包含SEQ ID NO:1中所示的核酸序列。
在第二个方面，本发明的AAV载体包含Proto 1S，所述Proto 1S示意性地描绘于图2B中并且包含SEQ ID NO:2中所示的核酸序列。
在第三个方面，本发明的AAV载体包含Proto 2S，所述Proto 2S示意性地描绘于图2C中并且包含SEQ ID NO:3中所示的核酸序列。
在第四个方面，本发明的AAV载体包含Proto 3S，所述Proto 3S示意性地描绘于图2D中并且包含SEQ ID NO:4中所示的核酸序列。
在另一个实施方案中，本发明的AAV载体包含一种核酸，所述核酸编码缺少包括SQ序列在内的整个B结构域和a3结构域的FVIII，所述a3结构域正位于轻链或C结构域的N末端。所述FVIII编码区序列是密码子优化的序列(参见Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1，该美国专利申请公开以引用的方式整体并入本文；以及McIntosh等,Blood 121:3335-3344,2013)。
在第一个方面，本发明的AAV载体包含Proto 4，所述Proto 4示意性地描绘于图3A中并且包含SEQ ID NO:5中所示的核酸序列。
在第二个方面，本发明的AAV载体包含Proto 5，所述Proto 5示意性地描绘于图3B中并且包含SEQ ID NO:6中所示的核酸序列。
在第三个方面，本发明的AAV载体包含Proto 6，所述Proto 6示意性地描绘于图3C中并且包含SEQ ID NO:7中所示的核酸序列。
在第四个方面，本发明的AAV载体包含Proto 7，所述Proto 7示意性地描绘于图3D中并且包含SEQ ID NO:8中所示的核酸序列。
在另一个实施方案中，本发明的AAV载体包含一种核酸，所述核酸包含AAV2 5'反向末端重复序列(ITR)、肝脏特异性转录调节区、密码子优化的功能活性FVIII编码区、任选的一个或多个内含子、多聚腺苷酸化序列、以及AAV2 3'ITR。在一个优选的实施方案中，所述肝脏特异性转录调节区包含缩短的ApoE增强子序列；具有186个碱基的人类α抗胰蛋白酶(hAAT)近端启动子，包括5'非翻译区(UTR)的42个碱基；以及选自由以下各项组成的组的一个或多个增强子：(i)具有34个碱基的人类ApoE/C1增强子、(ii)具有32个碱基的人类AAT启动子远端X区、以及(iii)人类AAT近端启动子的远端元件的80个额外的碱基；并且密码子优化的功能活性FVIII编码区编码FVIII SQ变体。在另一个优选的实施方案中，所述肝脏特异性转录调节区包含a1微球蛋白增强子序列和具有186个碱基的人类α抗胰蛋白酶(AAT)近端启动子。
在第一个方面，本发明的AAV载体包含构建体100ATG，所述构建体100ATG包含SEQID NO:9中所示的核酸序列。
在第二个方面，本发明的AAV载体包含构建体100ATG bGH多聚腺苷酸(poly A)，所述构建体100ATG bGH多聚腺苷酸包含SEQ ID NO:10中所示的核酸序列。
在第三个方面，本发明的AAV载体包含SEQ ID NO:11中所示的构建体100ATG短bGH多聚腺苷酸序列。
在第四个方面，本发明的AAV载体包含构建体103ATG，所述构建体103ATG包含SEQID NO:12中所示的核酸序列。
在第五个方面，本发明的AAV载体包含构建体103ATG短bGH多聚腺苷酸，所述构建体103ATG短bGH多聚腺苷酸包含SEQ ID NO:13中所示的核酸序列。
在第六个方面，本发明的AAV载体包含构建体105ATG bGH多聚腺苷酸，所述构建体105ATG bGH多聚腺苷酸包含SEQ ID NO:14中所示的核酸序列。
在第七个方面，本发明的AAV载体包含构建体DC172ATG FVIII，所述构建体DC172ATG FVIII包含SEQ ID NO:15中所示的核酸序列。
在第八个方面，本发明的AAV载体包含构建体DC172ATG FVIII hAAT，所述构建体DC172ATG FVIII hAAT包含SEQ ID NO:16中所示的核酸序列。
在第九个方面，本发明的AAV载体包含构建体DC172 2×HCR ATG FVIII，所述构建体DC172 2×HCR ATG FVIII包含SEQ ID NO:17中所示的核酸序列。
在第十个方面，本发明的AAV载体包含构建体DC172 2×HCR ATG FVIII hAAT，所述构建体DC172 2×HCR ATG FVIII hAAT包含SEQ ID NO:18中所示的核酸序列。
在第十一个方面，本发明的AAV载体包含构建体2×丝氨酸蛋白酶抑制蛋白A hAATATG FVIII，所述构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII包含SEQ ID NO:19中所示的核酸序列。
在第十二个方面，本发明的AAV载体包含构建体2×丝氨酸蛋白酶抑制蛋白A hAATATG FVIII 2×μ-球蛋白增强子，所述构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII2×μ-球蛋白增强子包含SEQ ID NO:20中所示的核酸序列。
在第十三个方面，本发明的AAV载体包含构建体100ATG短多聚腺苷酸2×μ-球蛋白增强子，所述构建体100ATG短多聚腺苷酸2×μ-球蛋白增强子包含SEQ ID NO:21中所示的核酸序列。
在第十四个方面，本发明的AAV载体包含构建体因子
VIII-BMN001，所述构建体因子VIII-BMN001包含SEQ ID NO:22中所示的核酸序列。
在第十五个方面，本发明的AAV载体包含SEQ ID NO:23中所示的构建体因子VIII-BMN002序列。
在第十六个方面，本发明的AAV载体包含构建体99，所述构建体99包含SEQ ID NO:24中所示的核酸序列。
在第十七个方面，本发明的AAV载体包含构建体100，所述构建体100包含SEQ IDNO:25中所示的核酸序列。
在第十八个方面，本发明的AAV载体包含构建体100反向定向，所述构建体100反向定向包含SEQ ID NO:26中所示的核酸序列。
在第十九个方面，本发明的AAV载体包含构建体100AT，所述构建体100AT包含SEQID NO:27中所示的核酸序列。
在第二十个方面，本发明的AAV载体包含构建体100AT2×MG，所述构建体100AT 2×MG包含SEQ ID NO:28中所示的核酸序列。
在第二十一个方面，本发明的AAV载体包含构建体100AT2×MG bGH多聚腺苷酸，所述构建体100AT 2×MG bGH多聚腺苷酸包含SEQ ID NO:29中所示的核酸序列。
在第二十二个方面，本发明的AAV载体包含构建体100AT2×MG(反向)bGH多聚腺苷酸，所述构建体100AT 2×MG(反向)bGH多聚腺苷酸包含SEQ ID NO:30中所示的核酸序列。
在第二十三个方面，本发明的AAV载体包含构建体100bGH多聚腺苷酸，所述构建体100bGH多聚腺苷酸包含SEQ ID NO:31中所示的核酸序列。
在第二十四个方面，本发明的AAV载体包含构建体100-400，所述构建体100-400包含SEQ ID NO:32中所示的核酸序列。
在第二十五个方面，本发明的AAV载体包含构建体101，所述构建体101包含SEQ IDNO:33中所示的核酸序列。
在第二十六个方面，本发明的AAV载体包含构建体102序列，所述构建体102序列包含SEQ ID NO:34中所示的核酸序列。
在第二十七个方面，本发明的AAV载体包含构建体103，所述构建体103包含SEQ IDNO:35中所示的核酸序列。
在第二十九个方面，本发明的AAV载体包含构建体103反向定向，所述构建体103反向定向包含SEQ ID NO:36中所示的核酸序列。
在第三十个方面，本发明的AAV载体包含构建体103AT，所述构建体103AT包含SEQID NO:37中所示的核酸序列。
在第三十一个方面，本发明的AAV载体包含构建体103AT2×MG，所述构建体103AT2×MG包含SEQ ID NO:38中所示的核酸序列。
在第三十二个方面，本发明的AAV载体包含构建体103AT2×MG bGH多聚腺苷酸，所述构建体103AT 2×MG bGH多聚腺苷酸包含SEQ ID NO:39中所示的核酸序列。
在第三十三个方面，本发明的AAV载体包含构建体103bGH多聚腺苷酸，所述构建体103bGH多聚腺苷酸包含SEQ ID NO:40中所示的核酸序列。
在第三十四个方面，本发明的AAV载体包含构建体104，所述构建体104包含SEQ IDNO:41中所示的核酸序列。
在第三十五个方面，本发明的AAV载体包含构建体105，所述构建体105包含SEQ IDNO:42中所示的核酸序列。
在第三十六个方面，本发明的AAV载体包含构建体106，所述构建体106包含SEQ IDNO:43中所示的核酸序列。
在第三十七个方面，本发明的AAV载体包含构建体106AT，所述构建体106AT包含SEQ ID NO:44中所示的核酸序列。
在第三十八个方面，本发明的AAV载体包含构建体2×丝氨酸蛋白酶抑制蛋白AhAAT，所述构建体2×丝氨酸蛋白酶抑制蛋白A hAAT包含SEQ ID NO:45中所示的核酸序列。
在另外的其它实施方案中，本发明涉及编码功能性因子VIII多肽的载体构建体，其中所述构建体以一个或多个不同的定向包含上述构建体和其组合的单个元件中的一个或多个。本发明还涉及呈相反定向的上述构建体。
呈单链形式的本发明的AAV载体具有小于约7.0kb的长度，或具有小于6.5kb的长度，或具有小于6.4kb的长度，或具有小于6.3kb的长度，或具有小于6.2kb的长度，或具有小于6.0kb的长度，或具有小于5.8kb的长度，或具有小于5.6kb的长度，或具有小于5.5kb的长度，或具有小于5.4kb的长度，或具有小于5.4kb的长度，或具有小于5.2kb的长度或具有小于5.0kb的长度。呈单链形式的本发明的AAV载体的长度在约5.0kb至约6.5kb的范围内，或长度在约4.8kb至约5.2k的范围内，或长度在4.8kb至5.3kb的范围内，或长度在约4.9kb至约5.5kb的范围内，或长度在约4.8kb至约6.0kb的范围内，或长度在约5.0kb至6.2kb的范围内或长度在约5.1kb至约6.3kb的范围内，或长度在约5.2kb至约6.4kb的范围内，或长度在约5.5kb至约6.5kb的范围内。
在另一个实施方案中，本发明提供了产生重组腺相关病毒(AAV)颗粒的方法，所述重组腺相关病毒(AAV)颗粒包含本发明的AAV载体中的任一种。所述方法包括以下步骤：培养已经转染了本发明的AAV载体中的任一种的细胞以及从转染的细胞的上清液中回收重组AAV。
本发明的细胞是易受杆状病毒感染的任何细胞类型，包括昆虫细胞，如HighFive、Sf9、Se301、SeIZD2109、SeUCR1、Sf9、Sf900+、Sf21、BTI-TN-5B1-4、MG-1、Tn368、HzAm1、BM-N、Ha2302、Hz2E5以及Ao38。所使用的优选的哺乳动物细胞可以是HEK293、HeLa、CHO、NS0、SP2/0、PER.C6、Vero、RD、BHK、HT 1080、A549、Cos-7、ARPE-19以及MRC-5细胞，并且包括哺乳动物细胞，如HEK293、HeLa、CHO、NS0、SP2/0、PER.C6、Vero、RD、BHK、HT 1080、A549、Cos-7、ARPE-19以及MRC-5细胞。
本发明还提供了包含本发明的AAV载体中的任一种的病毒颗粒或通过本发明的上述方法所产生的任何病毒颗粒。
“AAV病毒体”或“AAV病毒颗粒”或“AAV载体颗粒”指的是由至少一种AAV衣壳蛋白和衣壳化的多核苷酸AAV载体构成的病毒颗粒。如果所述颗粒包含异源多核苷酸(即除野生型AAV基因组以外的多核苷酸，如欲被递送到哺乳动物细胞中的转基因)，那么它通常被称为“AAV载体颗粒”或简称为“AAV载体”。因此，AAV载体颗粒的产生必然包括AAV载体的产生，以使得载体被包含在AAV载体颗粒内。
本发明还提供了包含本发明的AAV载体中的任一种的细胞以及由本发明的这些细胞产生的病毒颗粒。
在另一个实施方案中，本发明提供了治疗患有A型血友病的患者的方法，所述方法包括向所述患者施用有效量的本发明的AAV载体中的任一种或本发明的病毒颗粒或由本发明的方法产生的病毒颗粒。
在另一个实施方案中，本发明提供了本发明的AAV载体中的任一种用于制备用于治疗A型血友病的药物的用途。在一个方面，所述药物包含表达有效治疗A型血友病的量的人类FVIII的量的AAV载体。
在另一个实施方案中，本发明提供了一种组合物，所述组合物包含本发明的AAV载体中的任一种以用于治疗A型血友病。在一个方面，所述组合物包含表达有效治疗A型血友病的量的人类FVIII的量的AAV载体。
在另一个实施方案中，本发明的AAV载体用于产生可用于治疗患有A型血友病的患者的AAV病毒颗粒。
附图说明
图1提供了UCL SQ载体的示意图。从左至右，所述UCL SQ载体包含AAV2 5'ITR；野生型AAV2病毒序列；具有34个碱基的人类ApoE/C1增强子；具有32个碱基的人类AAT启动子远端X区；具有186个碱基的人类AAT启动子，包括5'UTR序列的42个碱基；密码子优化的人类FVIII SQ序列(参见Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1，该美国专利申请公开以引用的方式整体并入本文；以及McIntosh等,Blood121:3335-3344,2013)；具有49个碱基的合成多聚腺苷酸化序列；野生型AAV2病毒序列；以及AAV2 3'ITR。所述UCL SQ载体具有5081个碱基的长度。
图2提供了Proto 1载体、Proto 1S载体、Proto 2S载体以及Proto3S载体的示意图和序列。(A)Proto 1载体的示意图。从UCL SQ载体(参见图1)开始，使外部野生型AAV2病毒序列缺失，并且去除人类AAT 5'UTR与人类FVIII编码区之间和人类FVIII终止密码子与合成多聚腺苷酸化序列之间对应于限制性酶切位点的序列。(B)Proto 1S载体的示意图。从Proto 1载体开始，使AAV2 5'ITR的3'末端处的10个碱基和3'ITR的5'末端处的10个碱基缺失。(C)Proto 2S载体的示意图。从Proto 1S载体开始，将人类ApoE/C1增强子和人类AAT启动子远端X区移动到被***到人类FVIII序列的外显子1与2之间的具有100个碱基的合成内含子中。如由箭头所示，人类ApoE/C1增强子和人类AAT启动子远端X区的定向与它们在Proto 1S中的定向相比是反向的。(D)Proto 3S载体的示意图。从Proto 2S开始，将人类AAT启动子远端X区用人类ApoE/C1增强子的第二个拷贝以反向定向置换。
图3提供了Proto 4载体、Proto 5载体、Proto 6载体以及Proto 7载体的示意图。(A)Proto 4载体的示意图。从Proto 1载体开始，使SQ序列和a3结构域缺失。(B)Proto 5载体的示意图。从Proto 4载体开始，将具有129个碱基的FVIII内含子***于人类因子VIII序列的外显子1与2之间。(C)Proto 6载体的示意图。从Proto 5载体开始，将人类ApoE/C1增强子的第二个拷贝以正向定向***于FVIII内含子中。(D)Proto 7载体的示意图。从Proto5载体开始，将人类ApoE/C1增强子的第二个拷贝以反向定向***于FVIII内含子中。
图4A-图4KK提供了具有改进的启动子/增强子序列的AAV FVIII载体的示意图。(A)构建体100ATG的示意图。(B)构建体100ATG bGH多聚腺苷酸的示意图。(C)构建体100ATG短bGH多聚腺苷酸的示意图。(D)构建体103ATG的示意图。(E)构建体103ATG短bGH多聚腺苷酸的示意图。(F)构建体105ATG bGH多聚腺苷酸的示意图。(G)构建体DC172ATG FVIII的示意图。(H)构建体DC172ATG FVIII hAAT的示意图。(I)构建体DC172 2×HCR ATG FVIII的示意图。(J)构建体DC172 2×HCR ATG FVIII hAAT的示意图。(K)构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII的示意图。(L)构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATGFVIII2×μ-球蛋白增强子的示意图。(M)构建体100ATG短bGH多聚腺苷酸2×μ-球蛋白增强子的示意图。(N)构建体因子VIII-BMN001的示意图。(O)构建体FVIII-BMN002的示意图。(P)构建体99的示意图。(Q)构建体100的示意图。(R)构建体100反向定向的示意图。(S)构建体100AT的示意图。(T)构建体100AT 2×MG的示意图。(U)构建体100AT 2×MG bGH多聚腺苷酸的示意图。(V)构建体100AT 2×MG(反向)bGH多聚腺苷酸的示意图。(W)构建体100bGH多聚腺苷酸。(X)构建体100-400的示意图。(Y)构建体101的示意图。(Z)构建体102的示意图。(AA)构建体103的示意图。(BB)构建体103反向定向的示意图。(CC)构建体103AT的示意图。(DD)构建体103AT 2×MG的示意图。(EE)构建体103AT 2×MG bGH多聚腺苷酸的示意图。(FF)103sbGH多聚腺苷酸的示意图。(GG)构建体104的示意图。(HH)构建体105的示意图。(II)构建体106的示意图。(JJ)构建体106AT的示意图。(KK)构建体2×丝氨酸蛋白酶抑制蛋白A hAAT的示意图。
图5提供了在Rag2小鼠中评价Proto构建体的结果，并且证实Proto 1以类似于野生型的方式转导FVIII。
图6和7证实Proto 1、Proto 1S、Proto 2S以及Proto 3S表达VP1、VP2和VP3蛋白质(图5)以及VP1、VP2和VP3 DNA(图6)。
图8-10证实改进的启动子构建体提高了FVIII的表达。
具体实施方式
尺寸过大的AAV载体会在5'末端处被随机地截短并且缺少5'AAV ITR。由于AAV是单链DNA病毒并且包装正义链或反义链，因此尺寸过大的AAV载体中的正义链缺少5'AAVITR并且可能缺少靶蛋白编码基因的5'末端的部分，并且尺寸过大的AAV载体中的反义链缺少3'ITR并且可能缺少靶蛋白编码基因的3'末端的部分。功能性转基因在受尺寸过大的AAV载体感染的细胞中是通过正义截短型基因组和反义截短型基因组在靶细胞内的退火而产生的。
本发明提供了编码功能活性FVIII的AAV载体，即完全包装的AAV FVIII载体或具有高表达活性的AAV FVIII载体。本发明的AAV FVIII载体具有提高的表达/颗粒以及提高的AAV病毒产量和简化的纯化。将一个或多个内含子引入到FVIII蛋白质编码区中提高了表达。重新配置增强子的数目和定位也提高了表达。
UCL SQ载体
UCL SQ载体是一种尺寸过大，即大于5.0kb的AAV载体，该UCL SQ载体详细描述于Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1以及McIntosh等,Blood 121:3335-3344,2013中，该美国专利申请公开以引用的方式整体并入本文。如图1中所示，UCL SQ载体从左至右包含AAV血清2型(AAV2)5'ITR；野生型AAV2病毒序列；具有34个碱基的人类载脂蛋白E(ApoE)/C1增强子；具有32个碱基的人类α抗胰蛋白酶(AAT)启动子远端X区；具有186个碱基的人类AAT启动子，包括5'非翻译区(UTR)序列的42个碱基；密码子优化的人类FVIII序列，其中B结构域被具有14个氨基酸的SQ序列置换；具有49个碱基的合成多聚腺苷酸化序列；野生型AAV2病毒序列；以及AAV2 3'ITR。所述UCL SQ载体具有5081个碱基的长度。
如Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1以及McIntosh等,Blood 121:3335-3344,2013中所证实，所述UCL SQ载体在体外和体内表达功能活性FVIII。
Proto 1载体、Proto 1S载体、Proto 2S载体以及Proto 3S载体
为了避免尺寸过大的AAV载体的问题和/或为了提高AAV载体的表达，本发明提供了编码FVIII SQ变体的完全包装的更小，即小于5.0kb的AAV载体。Proto 1的序列的4970bp核苷酸序列示于SEQ ID NO:1中。
为了产生AAV载体Proto 1，与UCL SQ载体相比，使对于功能活性FVIII的产生来说似乎不必要的序列缺失。如实施例1中所示，去除外部DNA的110个碱基，包括AAV2 5'ITR的3'末端处的AAV2病毒序列的53个碱基、AAV2 3'ITR的5'末端处的AAV2病毒序列的46个碱基、以及与密码子优化的FVIII SQ编码区相邻的11个碱基。所得的Proto 1载体具有4970个碱基的长度。在设计时，Proto 1载体是否将能够在体外或体内表达功能性FVIII多肽是未知的。
为了产生AAV载体Proto 1S，从Proto 1载体去除AAV2 5'ITR的3'末端处的10个碱基、以及AAV32 3'ITR的5'末端处的10个碱基。所得的Proto 1S载体具有4950个碱基的长度。Proto 1S的序列的核苷酸序列示于SEQ ID NO:2中。
为了产生AAV载体Proto 2S，将合成的具有100个碱基的内含子***于Proto 1S载体中密码子优化的FVIII SQ序列的外显子1与2之间。将具有34个碱基的ApoE/C1增强子和具有32个碱基的人类AAT启动子远端X区从人类AAT启动子的上游去除并且以反向定向***所述合成内含子中(与在这些元件位于人类AAT启动子的上游时的定向相比)。所得的Proto2S载体具有4983个碱基的长度。Proto 2S的序列的核苷酸序列示于SEQ ID NO:3中。
为了产生AAV载体Proto 3S，将人类AAT启动子远端X区从Proto 2S载体中去除，并且用具有34个碱基的ApoE/C1增强子的第二个拷贝以反向定向置换。所得的Proto 3S载体具有4984个碱基的长度。Proto 3S的序列的核苷酸序列示于SEQ ID NO:4中。
Proto 4载体、Proto S载体、Proto 6载体以及Proto 7载体
为了减小AAV载体的尺寸和/或提高AAV载体的表达，本发明还提供了编码B结构域和a3结构域缺失的FVIII的完全包装的更小，即小于5.0kb的AAV载体。
为了产生AAV载体Proto 4，将具有14个氨基酸的SQ序列和位于与C结构域相邻处的a3结构域从Proto 1载体中去除。缺失的FVIII序列的总量是55个氨基酸或165个碱基。所得的Proto 4载体具有4805个碱基的长度。Proto 4的序列的核苷酸序列示于SEQ ID NO:5中。
为了产生AAV载体Proto 5，将具有129个碱基的截短型FVIII内含子***于Proto4载体中密码子优化的FVIII序列的外显子1与2之间。所得的Proto 5载体具有4934个碱基的长度。Proto 5的序列的核苷酸序列示于SEQ ID NO:6中。
为了产生AAV Proto 6载体，在Proto 5载体中将FVIII内含子的34个碱基用具有34个碱基的人类ApoE/C1增强子的第二个拷贝以正向定向置换。所得的Proto 6载体具有4934个碱基的长度。Proto 6的序列的核苷酸序列示于SEQ ID NO:7中。
为了产生AAV Proto 7载体，在Proto 5载体中将FVIII内含子的34个碱基用具有34个碱基的人类ApoE/C1增强子的第二个拷贝以反向定向置换。所得的Proto 7载体具有4934个碱基的长度。Proto 7的序列的核苷酸序列示于SEQ ID NO:8中。
具有改进的启动子/增强子序列的另外的AAV FVIII载体
产生具有强启动子的尺寸过大的AAV载体以提高B结构域和a3结构域缺失的FVIII的表达，并且这些构建体是用修饰的增强子和/或启动子序列产生的。在一些实施方案中，AAV FVIII载体表达截短型功能性FVIII。这些构建体包含一个或多个启动子和增强子序列，如ApoEHCR或其片段、μ-球蛋白增强子或其片段、人类α1抗胰蛋白酶启动子(hAAT)或其片段、丝氨酸蛋白酶抑制蛋白A增强子或其片段、LP1启动子增强子或其片段、或巨球蛋白增强子或其片段。这些构建体包含多聚腺苷酸化序列，如bGH多聚腺苷酸序列或合成兔β-球蛋白多聚腺苷酸序列。在某个实施方案中，所述构建体包含内含子或内含子的片段，如hAAT内含子或人类β-球蛋白内含子。
构建体100ATG具有5511个碱基的长度。这一构建体示于SEQ ID NO:9中，其中碱基1-145是5'AAV2 ITR，碱基160-502是ApoE HCR，碱基509-726是hAAT启动子，碱基727-910是修饰的人类β-球蛋白第二内含子，碱基923-5296是密码子优化的SQ FVIII，碱基5305-5352是合成兔β-球蛋白多聚腺苷酸并且碱基5367-5511是3'AAV2 ITR。
构建体100ATG bGH多聚腺苷酸具有5688个碱基的长度。这一构建体示于SEQ IDNO:10中，其中碱基1-145是5'AAV2 ITR，碱基160-502是ApoE HCR，碱基509-726是hAAT启动子，碱基727-910是修饰的人类β-球蛋白第二内含子，碱基923-5296是密码子优化的SQFVIII，碱基5305-5529是bGH多聚腺苷酸并且碱基5544-5688是3'AAV2 ITR。
构建体100ATG短bGH多聚腺苷酸具有5613个碱基的长度。这一构建体示于SEQ IDNO:11中，其中碱基1-145是5'AAV2 ITR，碱基160-502是ApoE HCR，碱基509-726是hAAT启动子，碱基727-910是修饰的人类β-球蛋白第二内含子，碱基923-5296是密码子优化的SQFVIII，碱基5305-5454是短bGH多聚腺苷酸并且碱基5469-5613是3'AAV2 ITR。
构建体103ATG具有5362个碱基的长度。这一构建体示于SEQ ID NO:12中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE重复序列的四个拷贝(4×)，碱基360-577是hAAT启动子，碱基578-761是修饰的人类β-球蛋白第二内含子，碱基774-5147是密码子优化的SQ FVIII，碱基5156-5203是合成兔β-球蛋白多聚腺苷酸并且碱基5218-5362是3'AAV2ITR。
构建体103ATG短bGH多聚腺苷酸具有5464个碱基的长度。这一构建体示于SEQ IDNO:13中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE重复序列的四个拷贝(4×)，碱基360-577是hAAT启动子，碱基578-761是修饰的人类β-球蛋白第二内含子，碱基774-5147是密码子优化的SQ FVIII，碱基5156-5305是bGH短多聚腺苷酸并且碱基5320-5464是3'AAV2 ITR。
构建体105ATG bGH多聚腺苷酸具有6354个碱基的长度。这一构建体示于SEQ IDNO:14中，其中碱基1-145是5'AAV2 ITR，碱基173-512是170bp微球蛋白增强子的两个拷贝(2×)，碱基519-736是hAAT启动子，碱基737-920是修饰的人类β-球蛋白第二内含子，碱基933-5306是密码子优化的SQ FVIII，碱基5315-5539是bGH多聚腺苷酸，碱基5546-6195是325bp ApoE HCR的两个拷贝(2×)并且碱基6210-6354是3'AAV2 ITR。
构建体DC172ATG FVIII具有6308个碱基的长度。这一构建体示于SEQ ID NO:15中，其中碱基1-145是5'AAV2 ITR，碱基160-449是145bp巨球蛋白增强子的两个拷贝(2×)，碱基450-1347是898bp hAAT启动子，碱基1348-1531是修饰的人类β-球蛋白第二内含子，碱基1544-5917是密码子优化的SQ FVIII，碱基5926-6149是bGH多聚腺苷酸并且碱基6164-6308是3'AAV2 ITR。
构建体DC172ATG FVIII hAAT具有5635个碱基的长度。这一构建体被示为SEQ IDNO:16，其中碱基1-145是5'AAV2 ITR，碱基160-449是145bp巨球蛋白增强子的两个拷贝(2×)，碱基457-674是hAAT启动子，碱基675-858是修饰的人类β-球蛋白第二内含子，碱基871-5244是密码子优化的SQ FVIII，碱基5253-5476是bGH多聚腺苷酸并且碱基5490-5635是3'AAV2 ITR。
构建体DC172 2×HCR ATG FVIII具有6962个碱基的长度。这一构建体示于SEQ IDNO:17中，其中碱基1-145是5'AAV2 ITR，碱基160-807是321bp ApoE HCR的两个拷贝(2×)，碱基814-1103是145bp巨球蛋白增强子的两个拷贝(2×)，碱基1104-2001是898bp hAAT启动子，碱基2002-2185是修饰的人类β-球蛋白第二内含子，碱基2198-6571是密码子优化的SQ FVIII，碱基6580-6803是bGH多聚腺苷酸并且碱基6818-6962是3'AAV2 ITR。
构建体DC172 2×HCR ATG FVIII hAAT具有6289个碱基的长度。这一构建体示于SEQ ID NO:18中，其中碱基1-145是5'AAV2 ITR，碱基160-807是321bp ApoE HCR的两个拷贝(2×)，碱基814-1103是145bp巨球蛋白增强子的两个拷贝(2×)，碱基1111-1328是hAAT启动子，碱基1329-1512是修饰的人类β-球蛋白第二内含子，碱基1525-5898是密码子优化的SQ FVIII，碱基5907-6130是bGH多聚腺苷酸并且碱基6245-6289是3'AAV2ITR。
构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII具有5430个碱基的长度。这一构建体示于SEQ ID NO:19中，其中碱基1-145是5'AAV2ITR，碱基168-309是71bp丝氨酸蛋白酶抑制蛋白A增强子的两个拷贝(2×)，碱基326-543是hAAT启动子，碱基544-727是修饰的人类β-球蛋白第二内含子，碱基740-5113是密码子优化的SQ FVIII，碱基5122-5271是短bGH多聚腺苷酸，并且碱基5286-5430是3'AAV2 ITR。
构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII 2×μ-球蛋白增强子具有5779个碱基的长度。这一构建体示于SEQ ID NO:20中，其中碱基1-145是5'AAV2 ITR，碱基168-309是71bp丝氨酸蛋白酶抑制蛋白A增强子的两个拷贝(2×)，碱基326-543是hAAT启动子，碱基544-727是修饰的人类β-球蛋白第二内含子，碱基740-5113是密码子优化的SQFVIII，碱基5122-5271是短bGH多聚腺苷酸，碱基5279-5618是170bpμ-球蛋白增强子的两个拷贝(2×)并且碱基5635-5779是3'AAV2 ITR。
构建体100ATG短bGH多聚腺苷酸2×μ-球蛋白增强子具有5962个碱基的长度。这一构建体示于SEQ ID NO:21中，其中碱基1-145是5'AAV2 ITR，碱基160-502是ApoE HCR，碱基509-726是hAAT启动子，碱基727-910是修饰的人类β-球蛋白第二内含子，碱基923-5296是密码子优化的SQ FVIII，碱基5305-5454是短bGH多聚腺苷酸，碱基5462-5801是170bp微球蛋白增强子的两个拷贝(2×)并且碱基5818-5962是3'AAV2 ITR。
构建体因子VIII-BMN001具有5919个碱基的长度。这一构建体示于SEQ ID NO:22中，其中碱基1-145是5'AAV2 ITR，碱基160-480是ApoE HCR，碱基487-884是398bp hAAT启动子，碱基885-1145是截短型hAAT内含子，碱基1155-5528是密码子优化的SQ FVIII，碱基5537-5760是bGH多聚腺苷酸并且碱基5775-5919是3'AAV2 ITR。
构建体FVIII-BMN002具有5306个碱基的长度。这一构建体示于SEQ ID NO:23中，其中碱基1-145是5'AAV2 ITR，碱基175-705是LP1启动子/增强子，碱基718-5091是密码子优化的SQ FVIII，碱基5100-5147是合成兔β-球蛋白多聚腺苷酸并且碱基5162-5306是3'AAV2 ITR。
构建体99具有5461个碱基的长度。这一构建体示于SEQ ID NO:24中，其中碱基1-145是5'AAV2 ITR，碱基169-627是ApoE HCR/MAR，碱基634-866是hAAT启动子，碱基873-5246是密码子优化的SQ FVIII，碱基5255-5302是合成兔β-球蛋白多聚腺苷酸并且碱基5317-5461是3'AAV2 ITR。
构建体100具有5327个碱基的长度。这一构建体示于SEQ ID NO:25中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基509-726是hAAT启动子，碱基739-5112是密码子优化的SQ FVIII，碱基5121-5168是合成兔β-球蛋白多聚腺苷酸并且碱基5183-5327是3'AAV2 ITR。
构建体100反向定向具有5309个碱基的长度。这一构建体示于SEQ ID NO:26中，其中碱基1-145是5'AAV2 ITR，碱基160-484是呈反向定向的ApoE HCR，碱基491-708是hAAT启动子，碱基721-5094是密码子优化的SQ FVIII，碱基5103-5150是合成兔β-球蛋白多聚腺苷酸并且碱基5165-5309是3'AAV2 ITR。
构建体100AT具有5532个碱基的长度。这一构建体示于SEQ ID NO:27中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基509-726是hAAT启动子，碱基727-931是hAAT内含子，碱基944-5317是密码子优化的SQ FVIII，碱基5326-5373是合成兔β-球蛋白多聚腺苷酸并且碱基5388-5532是3'AAV2 ITR。
构建体100AT 2×MG具有5877个碱基的长度。这一构建体示于SEQ ID NO:28中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基508-847是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基854-1071是hAAT启动子，碱基1072-1276是hAAT内含子，碱基1289-5662是密码子优化的SQ FVIII，碱基5671-5718是合成兔β-球蛋白多聚腺苷酸并且碱基5733-5877是3'AAV2 ITR。
构建体100AT 2×MG bGH多聚腺苷酸具有6054个碱基的长度。这一构建体示于SEQID NO:29中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基508-847是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基854-1071是hAAT启动子，碱基1072-1276是hAAT内含子，碱基1289-5662是密码子优化的SQ FVIII，碱基5671-5895是bGH多聚腺苷酸并且碱基5910-6054是3'AAV2 ITR。
构建体100AT 2×MG(反向)bGH多聚腺苷酸具有6054个碱基的长度。这一构建体示于SEQ ID NO:30中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基508-847是呈反向定向的170bpμ-球蛋白增强子的两个拷贝(2×)，碱基854-1071是hAAT启动子，碱基1072-1276是hAAT内含子，碱基1289-5662是密码子优化的SQ FVIII，碱基5671-5895是bGH多聚腺苷酸并且碱基5910-6054是3'AAV2 ITR。
构建体100bGH多聚腺苷酸具有5504个碱基的长度。这一构建体示于SEQ ID NO:31中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基509-726是hAAT启动子，碱基739-5112是密码子优化的SQ FVIII，碱基对5121-5345是bGH多聚腺苷酸并且碱基5360-5504是3'AAV2ITR。
构建体100-400具有5507个碱基的长度。这一构建体示于SEQ ID NO:32中，其中碱基1-145是5'AAV2 ITR，碱基169-493是ApoE HCR，碱基512-906是398bp hAAT启动子，碱基919-5292是密码子优化的SQ FVIII，碱基5301-5348是合成兔β-球蛋白多聚腺苷酸并且碱基5363-5507是3'AAV2ITR。
构建体101具有5311个碱基的长度。这一构建体示于SEQ ID NO:33中，其中碱基1-145是5'AAV2 ITR，碱基170-477是154bp ApoE HCR的两个拷贝(2×)，碱基493-710是hAAT启动子，碱基723-5096是密码子优化的SQ FVIII，碱基5105-5152是合成兔β-球蛋白多聚腺苷酸并且碱基5167-5311是3'AAV2 ITR。
构建体102具有5156个碱基的长度。这一构建体示于SEQ ID NO:34中，其中碱基1-145是5'AAV2 ITR，碱基169-322是154bp ApoE HCR，碱基338-555是hAAT启动子，碱基568-4941是密码子优化的SQ FVIII，碱基4950-4997是合成兔β-球蛋白多聚腺苷酸并且碱基5012-5156是3'AAV2ITR。
构建体103具有5178个碱基的长度。这一构建体示于SEQ ID NO:35中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE HCR的四个拷贝(4×)，碱基360-577是hAAT启动子，碱基590-4963是密码子优化的SQ FVIII，碱基4972-5019是合成兔β-球蛋白多聚腺苷酸并且碱基5034-5178是3'AAV2 ITR。
构建体103反向定向具有5160个碱基的长度。这一构建体示于SEQ ID NO:36中，其中碱基1-145是5'AAV2 ITR，碱基160-335是呈反向定向的44bp ApoE HCR的四个拷贝(4×)，碱基342-559是hAAT启动子，碱基572-4945是密码子优化的SQ FVIII，碱基4954-5001是合成兔β-球蛋白多聚腺苷酸并且碱基5016-5160是3'AAV2 ITR。
构建体103AT具有5383个碱基的长度。这一构建体示于SEQ ID NO:37中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE HCR的四个拷贝(4×)，碱基360-577是hAAT启动子，碱基578-782是hAAT内含子，碱基795-4374是密码子优化的SQ FVIII，碱基5177-5224是合成兔β-球蛋白多聚腺苷酸并且碱基5239-5383是3'AAV2 ITR。
构建体103AT 2×MG具有5728个碱基的长度。这一构建体示于SEQ ID NO:38中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE HCR的四个拷贝(4×)，碱基359-698是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基705-922是hAAT启动子，碱基923-1127是hAAT内含子，碱基1140-5513是密码子优化的SQ FVIII，碱基5522-5569是合成兔β-球蛋白多聚腺苷酸并且碱基5584-5728是3'AAV2 ITR。
构建体103AT 2×MG bGH多聚腺苷酸具有5905个碱基的长度。这一构建体示于SEQID NO:39中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE HCR的四个拷贝(4×)，碱基359-698是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基705-922是hAAT启动子，碱基923-1127是hAAT内含子，碱基1140-5513是密码子优化的SQ FVIII，碱基5522-5746是合成兔β-球蛋白多聚腺苷酸并且碱基5761-5905是5'AAV2 ITR。
构建体103bGH多聚腺苷酸具有5355个碱基的长度。这一构建体示于SEQ ID NO:40中，其中碱基1-145是5'AAV2 ITR，碱基169-344是44bp ApoE HCR的四个拷贝(4×)，碱基360-577是hAAT启动子，碱基590-4963是密码子优化的SQ FVIII，碱基4972-5196是合成兔β-球蛋白多聚腺苷酸并且碱基5211-5355是3'AAV2 ITR。
构建体104具有5618个碱基的长度。这一构建体示于SEQ ID NO:41中，其中碱基1-145是5'AAV2 ITR，碱基169-784是154bp ApoE HCR的四个拷贝(4×)，碱基800-1017是hAAT启动子，碱基1030-5403是密码子优化的SQ FVIII，碱基5412-5459是合成兔β-球蛋白多聚腺苷酸并且碱基5474-5618是3'AAV2 ITR。
构建体105具有5993个碱基的长度。这一构建体示于SEQ ID NO:42中，其中碱基1-145是5'AAV2 ITR，碱基173-512是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基519-736是hAAT启动子，碱基749-5122是密码子优化的SQ FVIII，碱基5131-5178是合成兔β-球蛋白多聚腺苷酸，碱基5185-5834是ApoE HCR的两个拷贝(2×)并且碱基5849-5993是3'AAV2 ITR。
构建体106具有5337个碱基的长度。这一构建体示于SEQ ID NO:43中，其中碱基1-145是5'AAV2 ITR，碱基173-512是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基519-736是hAAT启动子，碱基749-5122是密码子优化的SQ FVIII，碱基5131-5178是合成兔β-球蛋白多聚腺苷酸并且碱基5193-5337是3'AAV2 ITR。
构建体106AT具有5542个碱基的长度。这一构建体示于SEQ ID NO:44中，其中碱基1-145是5'AAV2 ITR，碱基173-512是170bpμ-球蛋白增强子的两个拷贝(2×)，碱基519-736是hAAT启动子，碱基737-941是hAAT内含子，碱基954-5327是密码子优化的SQ FVIII，碱基5336-5383是合成兔β-球蛋白多聚腺苷酸并且碱基5398-5542是3'AAV2 ITR。
构建体2×丝氨酸蛋白酶抑制蛋白A hAAT是5126个碱基。这一构建体示于SEQ IDNO:45中，其中碱基1-145是5'AAV2 ITR，碱基160-301是ApoE HCR，碱基308-525是hAAT启动子，碱基538-4911是密码子优化的SQ FVIII，碱基4920-4967是合成兔β-球蛋白多聚腺苷酸并且碱基4982-5126是3'AAV2 ITR。
AAV载体
如本文所用的术语“AAV”是腺相关病毒的标准缩写。腺相关病毒是只在细胞中生长的一种单链DNA细小病毒，其中某些功能由共感染辅助病毒提供。当前存在已经被表征的十三种AAV血清型，如下表1中所示。有关AAV的一般信息和综述可以见于例如Carter,1989,《细小病毒手册》
(Handbook of Parvoviruses),第1卷,第169-228页以及Berns,1990,《病毒学》(Virology),第1743-1764页,Raven Press,(New York)中。然而，完全预期的是，这些相同的原理将适用于另外的AAV血清型，这是因为公知的是，各种血清型在结构和功能这两方面，甚至是在基因水平上是非常密切相关的。(参见例如Blacklowe,1988,《细小病毒和人类疾病》(Parvoviruses and Human Disease)的第165-174页,J.R.Pattison编著；以及Rose,《综合病毒学》
(Comprehensive Virology)3:1-61(1974))。举例来说，所有AAV血清型显然表现出由同源rep基因所介导的非常相似的复制特性；并且全部均带有三种相关的衣壳蛋白，如AAV 6中表达的那些。相关度进一步通过异源双链体分析来表明，所述异源双链体分析揭示了血清型之间沿基因组长度的广泛的交叉杂交；以及对应于“反向末端重复序列”(ITR)的末端处类似的自退火段的存在。相似的感染模式还表明每一种血清型中的复制功能处在相似的调节控制之下。
如本文所用的“AAV载体”指的是包含由AAV末端重复序列(ITR)侧接的一个或多个所关注的多核苷酸(或转基因)的载体。当存在于已经被转染了编码和表达rep和cap基因产物的载体的宿主细胞中时，这样的AAV载体可以被复制和包装到感染性病毒颗粒中。
“AAV病毒体”或“AAV病毒颗粒”或“AAV载体颗粒”指的是由至少一种AAV衣壳蛋白和衣壳化的多核苷酸AAV载体构成的病毒颗粒。如果所述颗粒包含异源多核苷酸(即除野生型AAV基因组以外的多核苷酸，如欲被递送到哺乳动物细胞中的转基因)，那么它通常被称为“AAV载体颗粒”或简称为“AAV载体”。因此，AAV载体颗粒的产生必然包括AAV载体的产生，以使得载体被包含在AAV载体颗粒内。
AAV“rep”基因和“cap”基因分别是编码复制蛋白和衣壳化蛋白的基因。已经在迄今为止所研究的所有AAV血清型中发现了AAV rep基因和cap基因，并且所述基因描述于本文和所引用的参考文献中。在野生型AAV中，rep基因和cap基因一般被发现在病毒基因组中是彼此相邻的(即它们在一起“偶联”成邻接或重叠的转录单位)，并且它们在AAV血清型间一般是保守的。AAV rep基因和cap基因还被单独称为和统称为“AAV包装基因”。根据本发明的AAV cap基因编码Cap蛋白，所述Cap蛋白能够在rep和腺病毒辅助功能的存在下包装AAV载体并且能够结合靶细胞受体。在一些实施方案中，AAV cap基因编码具有源自于特定的AAV血清型，例如表1中所示的血清型的氨基酸序列的衣壳蛋白。
表1：AAV血清型
AAV血清型 基因库登录号
AAV-1 NC_002077.1
AAV-2 NC_001401.2
AAV-3 NC_001729.1
AAV-3B AF028705.1
AAV-4 NC_001829.1
AAV-5 NC_006152.1
AAV-6 AF028704.1
AAV-7 NC_006260.1
AAV-8 NC_006261.1
AAV-9 AX753250.1
AAV-10 AY631965.1
AAV-11 AY631966.1
AAV-12 DQ813647.1
AAV-13 EU285562.1
被用于产生AAV的AAV序列可以源自于任何AAV血清型的基因组。一般来说，AAV血清型具有在氨基酸水平和核酸水平上显著同源的基因组序列，提供一组相似的遗传功能，产生在物理上和功能上基本上等同的病毒体，并且通过几乎相同的机制复制和组装。对于AAV血清型的基因组序列和基因组相似性的论述，参见例如基因库登录号U89790；基因库登录号J01901；基因库登录号AF043303；基因库登录号AF085716；Chlorini等,J.Vir.71:6823-33(1997)；Srivastava等,J.Vir.45:555-64(1983)；Chlorini等,J.Vir.73:1309-1319(1999)；Rutledge等,J.Vir.72:309-319(1998)；以及Wu等,J.Vir.74:8635-47(2000)。
所有已知的AAV血清型的基因组组织是非常相似的。AAV的基因组是具有小于约5,000个核苷酸(nt)的长度的线性单链DNA分子。反向末端重复序列(ITR)侧接非结构复制(Rep)蛋白和结构(VP)蛋白的独特的编码核苷酸序列。VP蛋白形成衣壳。末端的145nt是自身互补的并且经过组织以使得可以形成在能量上稳定的分子内双链体，所述双链体形成T形发夹结构。这些发夹结构充当病毒DNA复制起点，从而用作细胞DNA聚合酶复合体的引物。Rep基因编码Rep蛋白，即Rep78、Rep68、Rep52、以及Rep40。Rep78和Rep68是从p5启动子转录的，并且Rep 52和Rep40是从p19启动子转录的。cap基因编码VP蛋白，即VP1、VP2以及VP3。cap基因是从p40启动子转录的。
在一些实施方案中，编码AAV衣壳蛋白的核酸序列与表达控制序列可操作地连接以在特定的细胞类型，如Sf9或HEK细胞中表达。可以使用本领域技术人员已知用于在昆虫宿主细胞或哺乳动物宿主细胞中表达外来基因的技术来实施本发明。用于在昆虫细胞中分子工程化和表达多肽的方法描述于例如以下文献中：Summers和Smith.1986.《用于杆状病毒载体和昆虫培养程序的方法手册》(A Manual of Methods for Baculovirus Vectorsand Insect Culture Procedures),德克萨斯州农业实验站(Texas AgriculturalExperimental Station),公告号(Bull.No.)7555,College Station,Tex.；Luckow.1991.Prokop等,在具有杆状病毒载体的昆虫细胞中异源基因的克隆和表达(Cloning and Expression of Heterologous Genes in Insect Cells withBaculovirus Vectors),《重组DNA技术和应用》(Recombinant DNA Technology andApplications),97-152；King,L.A.和R.D.Possee,1992,《杆状病毒表达***》(Thebaculovirus expression system),Chapman and Hall,United Kingdom；O'Reilly,D.R.,L.K.Miller,V.A.Luckow,1992,《杆状病毒表达载体：实验室手册》(BaculovirusExpression Vectors:A Laboratory Manual),New York；W.H.Freeman和Richardson,C.D.,1995,《杆状病毒表达方案》(Baculovirus Expression Protocols),《分子生物学方法》(Methods in Molecular Biology),第39卷；美国专利号4,745,051；US2003148506；以及WO 03/074714。对于编码AAV衣壳蛋白的核苷酸序列的转录特别合适的启动子是例如多角体启动子。然而，在昆虫细胞中具有活性的其它启动子是本领域已知的，例如p10、p35或IE-1启动子，并且还考虑了上述参考文献中所述的另外的启动子。
使用昆虫细胞来表达异源蛋白质是有据可查的，将核酸，如载体，例如昆虫细胞相容性载体引入到这样的细胞中的方法以及在培养中维持这样的细胞的方法也是如此。参见例如《分子生物学方法》(METHODS IN MOLECULAR BIOLOGY),Richard编著,Humana Press,NJ(1995)；O'Reilly等,《杆状病毒表达载体：实验室手册》(BACULOVIRUS EXPRESSIONVECTORS,A LABORATORY MANUAL),Oxford Univ.Press(1994)；Samulski等,J.Vir.63:3822-8(1989)；Kajigaya等,Proc.Nat'l.Acad.Sci.USA 88:4646-50(1991)；Ruffing等,J.Vir.66:6922-30(1992)；Kirnbauer等,Vir.219:37-44(1996)；Zhao等,Vir.272:382-93(2000)；以及Samulski等的美国专利号6,204,059。在一些实施方案中，在昆虫细胞中编码AAV的核酸构建体是昆虫细胞相容性载体。如本文所用的“昆虫细胞相容性载体”或“载体”指的是能够成效性转化或转染昆虫或昆虫细胞的核酸分子。示例性生物载体包括质粒、线性核酸分子、以及重组病毒。可以利用任何载体，只要它是昆虫细胞相容的即可。所述载体可以整合到昆虫细胞基因组中，但是载体在昆虫细胞中的存在不需要是永久性的并且还包括瞬时游离型载体。可以通过任何已知的手段，例如通过化学处理细胞、电穿孔、或感染来引入所述载体。在一些实施方案中，所述载体是杆状病毒、病毒载体、或质粒。在一个更优选的实施方案中，所述载体是杆状病毒，即所述构建体是杆状病毒载体。杆状病毒载体和它们的使用方法描述于上文引用的关于昆虫细胞的分子工程化的参考文献中。
杆状病毒是节肢动物的包膜DNA病毒，其中两个成员是用于在细胞培养物中产生重组蛋白的公知的表达载体。杆状病毒具有环状双链基因组(80kbp-200kbp)，所述基因组可以被工程化以允许向特定的细胞递送大的基因组含量。被用作载体的病毒一般是苜蓿丫纹夜蛾核型多角体病毒(Autographa californica multicapsid nucleopolyhedrovirus，AcMNPV)或家蚕(Bombyx mori，Bm)NPV(Kato等,2010)。
杆状病毒通常用于感染昆虫细胞以表达重组蛋白。具体来说，可以实现异源基因在昆虫中的表达，如例如以下文献中所述：美国专利号4,745,051；Friesen等(1986)；EP127,839；EP 155,476；Vlak等(1988)；Miller等(1988)；Carbonell等(1988)；Maeda等(1985)；Lebacq-Verheyden等(1988)；Smith等(1985)；Miyajima等(1987)；以及Martin等(1988)。可以用于产生蛋白质的许多杆状病毒株和变体以及相应的容许的昆虫宿主细胞描述于Luckow等(1988)；Miller等(1986)；Maeda等(1985)；以及McKenna(1989)中。
用于产生重组AAV的方法
本公开提供了用于在昆虫细胞或哺乳动物细胞中产生重组AAV的材料和方法。在一些实施方案中，病毒构建体还包含启动子和启动子下游的限制性酶切位点以允许***编码所关注的一种或多种蛋白质的多核苷酸，其中启动子和限制性酶切位点位于5'AAV ITR的下游和3'AAV ITR的上游。在一些实施方案中，病毒构建体还包含处于限制性酶切位点下游和3'AAV ITR上游的转录后调节元件。在一些实施方案中，病毒构建体还包含***于限制性酶切位点处并且与启动子可操作地连接的多核苷酸，其中所述多核苷酸包含所关注的蛋白质的编码区。如本领域技术人员所将了解的那样，本申请中所公开的AAV载体中的任一种可以作为病毒构建体用于所述方法中以产生重组AAV。
在一些实施方案中，辅助功能是由包含腺病毒或杆状病毒辅助基因的一种或多种辅助质粒或辅助病毒提供的。腺病毒或杆状病毒辅助基因的非限制性实例包括但不限于E1A、E1B、E2A、E4以及VA，它们可以为AAV包装提供辅助功能。
AAV的辅助病毒是本领域已知的并且包括例如来自腺病毒科(Adenoviridae)和疱疹病毒科(Herpesviridae)的病毒。AAV的辅助病毒的实例包括但不限于美国公开号20110201088(其公开内容以引用的方式并入本文)中所述的SAdV-13辅助病毒和SAdV-13样辅助病毒、辅助载体pHELP(Applied Viromics公司)。本领域技术人员将了解的是，可以为AAV提供足够的辅助功能的AAV的任何辅助病毒或辅助质粒均可以用于本文。
在一些实施方案中，AAV cap基因存在于质粒中。所述质粒还可以包含AAV rep基因。来自任何AAV血清型(包括但不限于AAV1、AAV2、AAV4、AAV5、AAV6、AAV7、AAV8、AAV9、AAV10、AAV11、AAV12、AAV13和其任何变体)的cap基因和/或rep基因可以用于本文中以产生重组AAV。在一些实施方案中，AAV cap基因编码来自血清1型、血清2型、血清4型、血清5型、血清6型、血清7型、血清8型、血清9型、血清10型、血清11型、血清12型、血清13型或其变体的衣壳。
在一些实施方案中，可以将昆虫细胞或哺乳动物细胞用辅助质粒或辅助病毒、编码AAV cap基因的病毒构建体和质粒转染；并且可以在共转染之后的各个时间点收集重组AAV病毒。举例来说，可以在共转染之后的约12小时、约24小时、约36小时、约48小时、约72小时、约96小时、约120小时、或这些时间点中的任何两个之间的时间收集重组AAV病毒。
还可以使用本领域已知的适用于产生感染性重组AAV的任何常规方法来产生重组AAV。在一些情况下，可以通过使用稳定表达对于AAV颗粒产生来说必要的组分中的一些的昆虫细胞或哺乳动物细胞来产生重组AAV。举例来说，包含AAV rep基因和cap基因以及选择标记，如新霉素抗性基因的质粒(或多种质粒)可以被整合到细胞的基因组中。然后可以将昆虫细胞或哺乳动物细胞用辅助病毒(例如提供辅助功能的腺病毒或杆状病毒)以及包含5'AAV ITR和3'AAV ITR(以及如果需要的话，编码异源蛋白质的核苷酸序列)的病毒载体共感染。这种方法的优势在于所述细胞是可选择的并且适用于大规模产生重组AAV。作为另一个非限制性实例，可以使用腺病毒或杆状病毒，而不是使用质粒来将rep基因和cap基因引入到包装细胞中。作为又另一个非限制性实例，含有5'AAV LTR和3'AAV LTR的病毒载体以及含有rep-cap基因的病毒载体这两者可以被稳定整合到生产细胞的DNA中，并且辅助功能可以由野生型腺病毒提供以产生重组AAV。
用于产生AAV的细胞类型
包含本发明的AAV载体的病毒颗粒可以使用允许产生AAV或生物产物并且可以在培养中维持的任何无脊椎动物细胞类型来产生。举例来说，所使用的昆虫细胞系可以来自于草地夜蛾(Spodoptera frugiperda)，如SF9、SF21、SF900+；果蝇细胞系；蚊子细胞系，例如白纹伊蚊(Aedes albopictus)衍生的细胞系；家蚕(domestic silkworm)细胞系，例如家蚕(Bombyx mori)细胞系；粉纹夜蛾(Trichoplusia ni)细胞系，如High Five细胞；或鳞翅目(Lepidoptera)细胞系，如黑巫蛾(Ascalapha odorata)细胞系。优选的昆虫细胞是来自易受杆状病毒感染的昆虫物种的细胞，包括High Five、Sf9、Se301、SeIZD2109、SeUCR1、Sf9、Sf900+、Sf21、BTI-TN-5B1-4、MG-1、Tn368、HzAm1、BM-N、Ha2302、Hz2E5以及Ao38。
杆状病毒是节肢动物的包膜DNA病毒，其中两个成员是用于在细胞培养物中产生重组蛋白的公知的表达载体。杆状病毒具有环状双链基因组(80kbp-200kbp)，所述基因组可以被工程化以允许向特定的细胞递送大的基因组含量。被用作载体的病毒一般是苜蓿丫纹夜蛾核型多角体病毒(AcMNPV)或家蚕(Bm-NPV)(Kato等,2010)。
杆状病毒通常用于感染昆虫细胞以表达重组蛋白。具体来说，可以实现异源基因在昆虫中的表达，如例如以下文献中所述：美国专利号4,745,051；Friesen等(1986)；EP127,839；EP 155,476；Vlak等(1988)；Miller等(1988)；Carbonell等(1988)；Maeda等(1985)；Lebacq-Verheyden等(1988)；Smith等(1985)；Miyajima等(1987)；以及Martin等(1988)。可以用于产生蛋白质的许多杆状病毒株和变体以及相应的容许的昆虫宿主细胞描述于Luckow等(1988)；Miller等(1986)；Maeda等(1985)；以及McKenna(1989)中。
在本发明的另一个方面，还使用允许AAV复制或生物产物产生并且可以在培养中维持的任何哺乳动物细胞类型来进行本发明的方法。所使用的优选的哺乳动物细胞可以是HEK293、HeLa、CHO、NS0、SP2/0、PER.C6、Vero、RD、BHK、HT 1080、A549、Cos-7、ARPE-19以及MRC-5细胞。
AAV FVIII载体的测试
测试本发明的完全包装的AAV FVIII载体的测定包括例如(1)在HepG2细胞中瞬时转染包含AAV载体核酸的双链DNA质粒以检验肝脏特异性mRNA的表达和剪接以及FVIII蛋白质的体外产生和分泌，所述HepG2细胞是一种源自于人类肝脏的细胞系；(2)在293细胞和受杆状病毒感染的昆虫细胞中产生包含AAV FVIII载体的AAV病毒体；(3)通过碱性凝胶分析和复制测定来评价AAV载体核酸；以及(4)在Rag2小鼠中评价FVIII表达、FVIII活性、以及FVIII比活性。这些测定更详细描述于实施例中。
本发明的完全包装的AAV FVIII载体展示出与UCL SQ载体至少相同的表达和/或活性，并且优选地与UCL SQ载体相比，展示出1.5倍、2倍、3倍、4倍、或5倍或更大的表达和/或活性。
本发明的完全包装的AAV FVIII载体具有高载体产量而很少有或没有片段基因组污染物，并且优选地与UCL SQ载体相比，具有1.5倍、2倍、3倍、4倍、或5倍的载体产量。
在考虑下列说明性实施例时，将了解本发明的其它方面和优势。
实施例
实施例1
Proto 1载体、Proto 1S载体、Proto 2S载体以及Proto 3S载体的产生
UCL SQ载体是一种尺寸过大，即大于5.0kb的AAV载体，该UCL SQ载体详细描述于Nathwani等的在2013年1月24日公开的美国专利申请公开号2013/0024960A1以及McIntosh等,Blood 121:3335-3344,2013中，该美国专利申请公开以引用的方式整体并入本文。如图1中所示，UCL SQ载体从左至右包含AAV血清2型(AAV2)5'ITR；野生型AAV2病毒序列；具有34个碱基的人类载脂蛋白E(ApoE)/C1增强子；具有32个碱基的人类α抗胰蛋白酶(AAT)启动子远端X区；具有186个碱基的人类AAT启动子，包括5'非翻译区(UTR)序列的42个碱基；密码子优化的人类FVIII序列，其中B结构域被具有14个氨基酸的SQ序列置换；具有49个碱基的合成多聚腺苷酸化序列；野生型AAV2病毒序列；以及AAV2 3'ITR。所述UCL SQ载体具有5081个碱基的长度。
为了获得小于UCL SQ载体的载体，从UCL SQ载体序列中去除由本文的发明人认为对于FVIII表达和/或活性或对于AAV病毒体产生来说不必要的DNA序列。去除外部DNA序列，包括AAV2 5'ITR的3'末端处的AAV2病毒序列的53个碱基、AAV2 3'ITR的5'末端处的AAV2病毒序列的46个碱基、以及与密码子优化的FVIII SQ编码区相邻的11个碱基。具有4970个碱基的长度的所得的Proto 1载体以示意形式示于图2A中，并且序列示于SEQ ID NO:1中。Proto 1产生感染性病毒并且编码功能性因子VIII多肽。
与AAV2 ITR中的发夹环相邻的序列在重组AAV载体中也可能是可有可无的(参见Srivastava等的美国专利号6,521,225；Wang等,J.Virol.70:1668-1677,1996；以及Wang等,J.Virol.71:3077-3082,1997)。为了进一步减小Proto 1载体的尺寸，直接在AAV2 5'ITR中的发夹环的3'末端处去除AAV2序列的10个碱基，并且直接在AAV2 3'ITR中的发夹环的5'末端处去除AAV2序列的10个碱基。具有4950个碱基的长度的所得的Proto 1S载体以示意形式示于图2B中，并且序列示于SEQ ID NO:2中。
为了努力提高Proto 1S载体中FVIII SQ变体的表达，将具有100个碱基的合成内含子***于密码子优化的FVIII序列中的外显子1与2之间。已知的是，***内含子可以使得本来含有更少的内含子的基因，例如像干扰素基因中mRNA的表达水平提高。
增强子被定义为以非距离和定向依赖性的方式起作用。对于FVIII表达来说，具有34个碱基的ApoE/C1增强子以非距离和定向依赖性方式起作用，如由它在Gray等的美国专利号8,030,065(FIX表达)和Nathwani等的美国专利申请公开号2013/0024960(FVIII表达)中假定的增强子活性所举例说明，这两篇文献以引用的方式整体并入本文。Di Simone等,EMBO J.6:2759-2766,1987中所述的具有32个碱基的人类AAT启动子远端X区位于提高异源启动子的表达的调节结构域内。
再次试图进一步提高Proto 1S载体中FVIII SQ变体的表达，所述合成内含子序列纳入具有34个碱基的人类ApoE/C1增强子和具有32个碱基的人类AAT启动子远端X区，后者是从它在人类AAT启动子上游的位置移动而来的。这两个调节元件以相对于它们在Proto1S中的定向反向的定向***。具有4983个碱基的长度的所得的Proto 2S载体以示意形式示于图2C中，并且序列示于SEQ ID NO:3中。
由于人类AAT启动子远端X区先前并没有被证实在内含子中的转录起始位点的下游起作用，因此将Proto 2S载体中的这个调节元件用具有34个碱基的人类ApoE/C1增强子的第二个拷贝以与内含子中所述增强子的第一个拷贝相同的定向置换。具有4985个碱基的长度的所得的Proto 3S载体以示意形式示于图2D中，并且序列示于SEQ ID NO:4中。
将Proto 1、Proto 1S、Proto 2S以及Proto 3S载体核酸克隆到pUC19细菌表达质粒中，从而产生AAV FVIII载体的双链形式。
实施例2
Proto 4载体、Proto 5载体、Proto 6载体以及Proto 7载体的产生
为了进一步减小Proto 1载体的尺寸和/或与Proto 1载体相比提高FVIII的表达，使位于与轻链或C结构域相邻处的a3结构域缺失。a3结构域涉及与血管性血友病因子(vonWillenbrand Factor)的结合，但是在体内对于功能活性FVIII来说可能是可有可无的。
从Proto 1载体开始，使具有14个氨基酸的SQ序列和具有41个氨基酸的a3结构域(对应于野生型FVIII的氨基酸1649-1689)缺失。具有4805个碱基的长度的所得的Proto 4载体以示意形式示于图3A中，并且序列示于SEQ ID NO:5中。
为了试图提高B结构域和a3结构域缺失的FVIII的表达，将具有129个碱基的截短型FVIII内含子***于Proto 4载体中密码子优化的FVIII序列中的外显子1与2之间。具有4934个碱基的长度的所得的Proto 5载体以示意形式示于图3B中，并且序列示于SEQ IDNO:6中。
为了试图进一步提高B结构域和a3结构域缺失的FVIII的表达，在Proto 5载体中将具有34个碱基的人类ApoE/C1增强子的第二个拷贝以正向定向或反向定向***。具有4934个碱基的长度并且具有呈正向定向的内含子ApoE/C1增强子的所得的Proto 6载体以示意形式示于图3C中，并且序列示于SEQ ID NO:7中。
具有4934个碱基的长度并且具有呈反向定向的内含子ApoE/C1增强子的所得的Proto 7载体以示意形式示于图3D中，并且序列示于SEQ ID NO:8中。
将Proto 4、Proto 5、Proto 6以及Proto 7载体核酸克隆到pUC19细菌表达质粒中，从而产生AAV FVIII载体的双链形式。
实施例3
测试AAV FVIII载体的表达和活性的测定
测试本发明的AAV FVIII载体的测定包括例如(1)在HepG2细胞中瞬时转染包含AAV载体核酸的双链DNA质粒以检验肝脏特异性mRNA的表达和剪接以及FVIII蛋白质的体外产生和分泌，所述HepG2细胞是一种源自于人类肝脏的细胞系；(2)在293细胞和受杆状病毒感染的昆虫细胞中产生包含AAV FVIII载体的AAV病毒体；(3)通过碱性凝胶分析和复制测定来评价AAV载体核酸；以及(4)在Rag2小鼠中评价FVIII表达、FVIII活性、以及FVIII比活性。
瞬时转染测定。
进行初步体外测定以将来自本发明的AAV FVIII载体的FVIII表达和活性与来自UCL SQ载体的FVIII表达和活性进行比较。将本发明的AAV FVIII载体的双链形式瞬时转染到人类肝脏细胞系HepG2中。在转染之后，例如24小时或48小时后，测量培养上清液中的FVIII抗原和活性。
使用这种测定，瞬时转染了Proto 1载体、Proto 1S载体以及Proto2S载体的HepG2细胞中的FVIII活性类似于使用UCL SQ载体所获得的FVIII活性，这证实Proto 1载体、Proto 1S载体以及Proto 2S载体能够表达功能性因子VIII蛋白质。
在293细胞和受杆状病毒感染的昆虫细胞中AAV病毒体的产生。
为了证实本发明的AAV FVIII载体的确包装了编码FVIII的核酸，将如实施例1和2中所述而产生的AAV FVIII载体的双链形式引入到能够产生AAV病毒体的细胞中。在第一AAV病毒产生***中，将包含呈双链形式的AAV FVIII载体核酸的质粒连同表达AAV Cap和Rep蛋白的质粒和表达为AAV病毒体产生所需的腺病毒辅助功能的质粒一起共转染到293细胞中。在第二AAV病毒产生***中，产生表达AAV FVIII载体核酸以及AAV Cap和Rep蛋白的杆状病毒构建体，然后将它们共感染到昆虫Sf9细胞中。通过本领域已知的标准方法对在瞬时转染的293细胞或受杆状病毒感染的Sf9细胞中产生的所得的AAV病毒体进行纯化和分析。
通过碱性凝胶和复制测定进行评价
使用碱性凝胶电泳测定来确定包装的核酸的尺寸。使用复制中心测定来确定哪些AAV FVIII载体通过这两种包装方法以完整形式被包装。
使用引物延伸测定来定量具有完整末端，即终止于AAV2 5'ITR(正义链)或3'ITR(反义链)中的发夹环的5'末端的AAV FVIII载体核酸的量。
或者，使用PCR测定来确定AAV FVIII载体核酸是否具有完整末端，即终止于AAV25'ITR(正义链)或3'ITR(反义链)中的发夹环的5'末端。
在Rag2小鼠中进行评价
在Rag2小鼠中以静脉内给予的2e11个、2e12个、以及2e13个病毒基因组(vg)/公斤针对FVIII表达和活性测试在瞬时转染的293细胞或受杆状病毒感染的Sf9细胞中由包装的载体产生的AAV病毒体。在这一测定中使用Rag2小鼠，这是因为FVIII表达和/或活性不会因宿主对AAV病毒或人类FVIII蛋白质的免疫反应的存在而变得复杂。
使用基于ELISA的测定确定FVIII抗原。使用FXa激活测定和/或凝血测定来确定FVIII活性。使用FVIII抗原和活性测定确定FVIII比活性。
在实施本发明中许多改动方案和变化方案预期在考虑本发明的优选的实施方案时被本领域技术人员想到。因此，应当对本发明的范围所施加的限制条件仅是所附权利要求书中出现的那些限制条件。
实施例4
具有改进的启动子/增强子序列的构建体的产生
为了产生提高功能性FVIII表达的具有强启动子的另外的AAV载体，用修饰的增强子和/或启动子序列产生构建体。在一些实施方案中，所述构建体包含ApoE增强子或μ-球蛋白增强子的缩短型式。使用标准DNA克隆技术产生这些构建体并且其序列示于SEQ IS NO:9-45中。
实施例5
AAV病毒颗粒的产生
重组杆粒的产生
将DH10 Bac感受态细胞在冰上解冻。添加重组穿梭质粒(例如pFB-GFP)并且与感受态细胞轻轻混合并且在冰上孵育30分钟。然后在约42℃的温度下对感受态细胞进行加热，持续30秒，然后在冰上冷却2分钟。在42℃使感受态细胞进行热休克，持续30秒，并且在冰上冷却2分钟。将SOC添加到细胞中并且在37℃在搅拌下孵育4小时以允许进行重组。在孵育期间，将X-gal涂铺到两个LB板(另外含有各种抗生素(例如卡那霉素(kanamycin)、庆大霉素(gentamycin)以及四环素(tetracycline))上以用于转化，之后涂铺IPTG。
获得一定量的孵育混合物，将它稀释，然后涂铺到两个LB板上并且在37℃孵育约30小时-48小时。从每一个板选择若干个白色菌落并且在含有LB板中提供的相同抗生素组合的LB培养基中培养过夜。随后，制备杆粒DNA和甘油原液并且储存在-80℃。
重组杆粒DNA的纯化
将一定量的杆粒甘油原液取出，并且接种到含有实施例1中所述的LB板中所提供的相同抗生素组合的LB培养基中。使培养物在37℃在振荡下生长过夜。随后，使一定量的培养物在微型离心机中以全速旋转约30秒。
使用移液管将沉淀物重悬于重悬缓冲液中，继而重悬于裂解缓冲液中，并且将管翻转若干次以混合缓冲液，然后在室温下孵育约5分钟。示例性重悬缓冲液包含50mM Tris-CL(pH 8.0)、10mM EDTA以及100μg/mL RNA酶A。示例性裂解缓冲液包含200mM的NaOH和1％SDS。缓慢添加一定量的沉淀缓冲液(例如包含3.0M乙酸钾(pH 5.5)的缓冲液)并且将管翻转若干次以混合缓冲液，然后在冰上孵育约10分钟。将管以全速离心约10分钟并且将上清液倒入容纳异丙醇的管中。将管翻转若干次以混合溶液。
随后，在室温下将溶液以全速离心约15分钟并且在离心之后立即用移液管去除上清液。
添加一定量的70％乙醇以冲洗沉淀物并且再次以全速旋转1分钟。然后去除乙醇并且将溶液再次旋转以去除痕量的乙醇。将一定量的TE/EB缓冲液添加到每一个管中并且小心地通过移液管溶解沉淀物。如果不立即使用的话，将溶液储存在-20℃。
重组杆状病毒的P0原液的产生
将Sf9细胞以约1×106个细胞/孔接种到6孔板中(或以6×106个细胞接种于10cm板中或以1.7×107个细胞接种于15cm培养皿中)并且在转染之前使细胞贴壁至少1小时。
如下制备转染溶液A和B：溶液A：在15mL管中将一定量的杆粒稀释到一定量不含抗生素的无血清培养基中。溶液B：在15mL管中将一定量的CellFectin稀释到一定量不含抗生素的无血清培养基中。将溶液B添加到溶液A中并且通过移液管吹吸约3次轻轻混合，并且在室温下孵育30分钟-45分钟。随后，从板中吸出培养基并且添加一定量的不含抗生素的无血清培养基以洗涤细胞。将一定量的不含抗生素的SF900II添加到容纳脂质-DNA混合物的每一个管中。
从细胞吸出培养基，将转染溶液添加到细胞中并且将细胞在28℃孵育约5小时。去除转染溶液并且添加一定量的无血清培养基+抗生素，并且在28℃孵育约4天。将含有重组杆状病毒的培养基收集并且以1000rpm旋转约5分钟以去除细胞碎片。将杆状病毒在暗处储存在4℃。
杆状病毒(P1)的扩增
将Sf9细胞培养到约4×106个细胞/毫升并且在摇瓶中使用新鲜培养基稀释到约2×106个细胞/毫升。将一定量的Sf9细胞用一定量的P0储备杆状病毒感染。感染复数(MOI)是约0.1。
将Sf9细胞孵育约3天并且收集杆状病毒。将细胞以2,000rpm旋转5分钟以使细胞沉淀并且收集上清液并且在暗处储存在4℃。根据Clontech公司的快速滴度试剂盒(RapidTiter Kit)方案测定杆状病毒的滴度。
使用P1重组杆状病毒产生AAV
将Sf9细胞培养到约1×107个细胞/毫升并且稀释到约5×106个细胞/毫升。将一定量的稀释了的Sf9细胞用Bac-载体(5Moi)和Bac-辅助病毒(15Moi)感染3天。在第三天评估细胞活力(观测到约50％-70％的死细胞)。
通过以3000rpm离心10分钟来收集细胞沉淀物。去除培养基并且将细胞裂解(或如果不立即使用的话，将细胞沉淀物储存在-20℃)。
裂解和显带方案
将一定量的Sf9裂解缓冲液加上Benzonase(核酸酶)添加到每一个细胞沉淀物中并且充分涡旋混合以使细胞重悬。将重悬的Sf9细胞在冰上孵育约10分钟以将裂解物冷却。将裂解物超声处理约20秒以将细胞充***解，然后在37℃孵育约30分钟。
添加一定量的5M NaCl并且将混合物涡旋混合，然后在37℃再孵育30分钟。添加一定量的NaCl以使盐浓度达到约500mM，涡旋混合并且在15℃以8,000rpm离心20分钟以产生澄清的裂解物。
对澄清的裂解物进行超速离心步骤。通过经由具有长针的注射器首先添加澄清的裂解物，然后添加1.32g/cc的量和1.55g/cc的量的CsCl溶液来制备CsCl梯度。标出CsCl溶液之间的界面。将PBS添加到离心管的顶部上并且小心地将管平衡和密封。
在15℃将管以55,000rpm离心约20小时。在每一个管的顶部上刺穿孔并且标出位于略高于两种CsCl溶液的界面标记处的AAV条带。
通过以下步骤进行第二次CsCl离心：将AAV溶液转移到用于70.1Ti转子的离心管中，并且将一定量的CsCl溶液添加到靠近管顶部处。将管平衡和密封。将管以65,000rpm离心约20小时并且收集AAV条带(更低的条带，更高的条带是空衣壳)。
实施例5
在Rag2小鼠中评价构建体
使用杆状病毒和293细胞，使用UCL SQ构建体、Proto 1构建体、Proto S1构建体、Proto S2构建体以及Proto S3构建体产生包含密码子优化的SQ FVIII编码基因序列的AAV基因组。包装限度对于杆状病毒来说是4800bp并且对于293细胞来说是4950bp。
如图5中所示，具有截短型或非截短型基因组的Proto 1以类似于UCL SQ构建体的方式转导FVIII。在4％-12％Bis-Tris凝胶上测量由杆状病毒和293T细胞裂解物产生的AAV5.2。每一种样品均表达VP1、VP2以及VP3蛋白质，如图6中所示。使来自AAV样品的基因组DNA在0.8％碱性琼脂糖凝胶上进行电泳，如图7中所示。
当通过杆状病毒***制备这些AAV时，Proto 1的转导类似于UCL SQ构建体。含有内含子的纳入的Proto 2S和Proto 3S的转导没有优于Proto 1。在293细胞中制备的含有AAV侧接序列的UCL SQ载体比在杆状病毒中制备的缺少AAV序列的UCL SQ更强效。因此，将另外的增强子添加到Proto 1中，例如构建体101、102、102以及104以试图提高效能。
实施例6
具有改进的启动子/增强子序列的AAV FVIII载体的表达和活性
使用液压注射方案测试包含构建体99至构建体106的AAV载体的表达和活性。液压递送是一种在体内筛选肝脏启动子的快速的方法。使用实施例5中所述的方法产生AAV质粒DNA，然后在TransIT-QR液压递送溶液中稀释。将质粒DNA以通过(小鼠体重(g)/10)＝0.1ml递送溶液)所确定的体积注射到5周-6周大的C57Bl/6小鼠(18g-25g)的尾静脉中。注射时间短于5秒。在注射之后48小时采集来自每一只小鼠的血浆并且使用ELISA测定测量所表达的FVIII抗原的量。
将递增剂量的Proto 1质粒(2.5μg、5μg、12.5μg以及50μg)注射到小鼠的尾静脉中。使用ELISA测试测量被注射的小鼠的血浆中FVIII的量并且使用重组FVIII(Xyntha SQ等同物)作为标准以进行比较。
为了研究表达，构建体p100-400、构建体100(p100)、构建体FVIII-BMN001(pFVIII-BMN001)、Proto 1、构建体100AT(p100-AT)、构建体100bGH多聚腺苷酸(p100-bGHPA)、构建体101(p101)以及构建体104(p104)的改进的启动子/增强子元件。如图8中所示，所有的构建体以不同的效率水平产生功能性FVIII。
图9和10提供了注射1μg的各种构建体的质粒的数据。如图8中所示，注射构建体FVIII-BMN001、构建体FVIII-BMN002、构建体102(p102)、构建体103(p103)以及构建体104(p104)使得在10只小鼠中有5只中表达至少20ng的FVIII。如图9中所示，注射构建体FVIII-BMN001、构建体103(p103)、构建体103-AT(p103-AT；398bp hAAT启动子)、构建体100(p100)、构建体100AT(p100-AT；398bp hAAT启动子)使得在10只小鼠中有5只中表达至少100ng/ml的FVIII。
序列表
<110> 生物马林药物股份有限公司（BIOMARIN PHARMACEUTICAL INC.）
伦敦大学学院(UNIVERSITY COLLEGE LONDON)
<120> 腺相关病毒因子VIII载体
<130> 30610/48689
<150> US-61/877,042
<151> 2013-09-12
<160> 45
<170> PatentIn 3.5版
<210> 1
<211> 4970
<212> DNA
<213> 腺相关病毒
<400> 1
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgtttg ctgcttgcaa tgtttgccca ttttagggtg 180
gacacaggac gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta 240
gcccctgttt gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc 300
cccgttgccc ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca 360
gcttcaggca ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc 420
acctgcttct tcctgtgcct gctgaggttc tgcttctctg ccaccaggag atactacctg 480
ggggctgtgg agctgagctg ggactacatg cagtctgacc tgggggagct gcctgtggat 540
gccaggttcc cccccagagt gcccaagagc ttccccttca acacctctgt ggtgtacaag 600
aagaccctgt ttgtggagtt cactgaccac ctgttcaaca ttgccaagcc caggcccccc 660
tggatgggcc tgctgggccc caccatccag gctgaggtgt atgacactgt ggtgatcacc 720
ctgaagaaca tggccagcca ccctgtgagc ctgcatgctg tgggggtgag ctactggaag 780
gcctctgagg gggctgagta tgatgaccag accagccaga gggagaagga ggatgacaag 840
gtgttccctg ggggcagcca cacctatgtg tggcaggtgc tgaaggagaa tggccccatg 900
gcctctgacc ccctgtgcct gacctacagc tacctgagcc atgtggacct ggtgaaggac 960
ctgaactctg gcctgattgg ggccctgctg gtgtgcaggg agggcagcct ggccaaggag 1020
aagacccaga ccctgcacaa gttcatcctg ctgtttgctg tgtttgatga gggcaagagc 1080
tggcactctg aaaccaagaa cagcctgatg caggacaggg atgctgcctc tgccagggcc 1140
tggcccaaga tgcacactgt gaatggctat gtgaacagga gcctgcctgg cctgattggc 1200
tgccacagga agtctgtgta ctggcatgtg attggcatgg gcaccacccc tgaggtgcac 1260
agcatcttcc tggagggcca caccttcctg gtcaggaacc acaggcaggc cagcctggag 1320
atcagcccca tcaccttcct gactgcccag accctgctga tggacctggg ccagttcctg 1380
ctgttctgcc acatcagcag ccaccagcat gatggcatgg aggcctatgt gaaggtggac 1440
agctgccctg aggagcccca gctgaggatg aagaacaatg aggaggctga ggactatgat 1500
gatgacctga ctgactctga gatggatgtg gtgaggtttg atgatgacaa cagccccagc 1560
ttcatccaga tcaggtctgt ggccaagaag caccccaaga cctgggtgca ctacattgct 1620
gctgaggagg aggactggga ctatgccccc ctggtgctgg cccctgatga caggagctac 1680
aagagccagt acctgaacaa tggcccccag aggattggca ggaagtacaa gaaggtcagg 1740
ttcatggcct acactgatga aaccttcaag accagggagg ccatccagca tgagtctggc 1800
atcctgggcc ccctgctgta tggggaggtg ggggacaccc tgctgatcat cttcaagaac 1860
caggccagca ggccctacaa catctacccc catggcatca ctgatgtgag gcccctgtac 1920
agcaggaggc tgcccaaggg ggtgaagcac ctgaaggact tccccatcct gcctggggag 1980
atcttcaagt acaagtggac tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg 2040
tgcctgacca gatactacag cagctttgtg aacatggaga gggacctggc ctctggcctg 2100
attggccccc tgctgatctg ctacaaggag tctgtggacc agaggggcaa ccagatcatg 2160
tctgacaaga ggaatgtgat cctgttctct gtgtttgatg agaacaggag ctggtacctg 2220
actgagaaca tccagaggtt cctgcccaac cctgctgggg tgcagctgga ggaccctgag 2280
ttccaggcca gcaacatcat gcacagcatc aatggctatg tgtttgacag cctgcagctg 2340
tctgtgtgcc tgcatgaggt ggcctactgg tacatcctga gcattggggc ccagactgac 2400
ttcctgtctg tgttcttctc tggctacacc ttcaagcaca agatggtgta tgaggacacc 2460
ctgaccctgt tccccttctc tggggagact gtgttcatga gcatggagaa ccctggcctg 2520
tggattctgg gctgccacaa ctctgacttc aggaacaggg gcatgactgc cctgctgaaa 2580
gtctccagct gtgacaagaa cactggggac tactatgagg acagctatga ggacatctct 2640
gcctacctgc tgagcaagaa caatgccatt gagcccagga gcttcagcca gaacccccca 2700
gtgctgaaga ggcaccagag ggagatcacc aggaccaccc tgcagtctga ccaggaggag 2760
attgactatg atgacaccat ctctgtggag atgaagaagg aggactttga catctacgac 2820
gaggacgaga accagagccc caggagcttc cagaagaaga ccaggcacta cttcattgct 2880
gctgtggaga ggctgtggga ctatggcatg agcagcagcc cccatgtgct gaggaacagg 2940
gcccagtctg gctctgtgcc ccagttcaag aaggtggtgt tccaggagtt cactgatggc 3000
agcttcaccc agcccctgta cagaggggag ctgaatgagc acctgggcct gctgggcccc 3060
tacatcaggg ctgaggtgga ggacaacatc atggtgacct tcaggaacca ggccagcagg 3120
ccctacagct tctacagcag cctgatcagc tatgaggagg accagaggca gggggctgag 3180
cccaggaaga actttgtgaa gcccaatgaa accaagacct acttctggaa ggtgcagcac 3240
cacatggccc ccaccaagga tgagtttgac tgcaaggcct gggcctactt ctctgatgtg 3300
gacctggaga aggatgtgca ctctggcctg attggccccc tgctggtgtg ccacaccaac 3360
accctgaacc ctgcccatgg caggcaggtg actgtgcagg agtttgccct gttcttcacc 3420
atctttgatg aaaccaagag ctggtacttc actgagaaca tggagaggaa ctgcagggcc 3480
ccctgcaaca tccagatgga ggaccccacc ttcaaggaga actacaggtt ccatgccatc 3540
aatggctaca tcatggacac cctgcctggc ctggtgatgg cccaggacca gaggatcagg 3600
tggtacctgc tgagcatggg cagcaatgag aacatccaca gcatccactt ctctggccat 3660
gtgttcactg tgaggaagaa ggaggagtac aagatggccc tgtacaacct gtaccctggg 3720
gtgtttgaga ctgtggagat gctgcccagc aaggctggca tctggagggt ggagtgcctg 3780
attggggagc acctgcatgc tggcatgagc accctgttcc tggtgtacag caacaagtgc 3840
cagacccccc tgggcatggc ctctggccac atcagggact tccagatcac tgcctctggc 3900
cagtatggcc agtgggcccc caagctggcc aggctgcact actctggcag catcaatgcc 3960
tggagcacca aggagccctt cagctggatc aaggtggacc tgctggcccc catgatcatc 4020
catggcatca agacccaggg ggccaggcag aagttcagca gcctgtacat cagccagttc 4080
atcatcatgt acagcctgga tggcaagaag tggcagacct acaggggcaa cagcactggc 4140
accctgatgg tgttctttgg caatgtggac agctctggca tcaagcacaa catcttcaac 4200
ccccccatca ttgccagata catcaggctg caccccaccc actacagcat caggagcacc 4260
ctgaggatgg agctgatggg ctgtgacctg aacagctgca gcatgcccct gggcatggag 4320
agcaaggcca tctctgatgc ccagatcact gccagcagct acttcaccaa catgtttgcc 4380
acctggagcc ccagcaaggc caggctgcac ctgcagggca ggagcaatgc ctggaggccc 4440
caggtcaaca accccaagga gtggctgcag gtggacttcc agaagaccat gaaggtgact 4500
ggggtgacca cccagggggt gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg 4560
atcagcagca gccaggatgg ccaccagtgg accctgttct tccagaatgg caaggtgaag 4620
gtgttccagg gcaaccagga cagcttcacc cctgtggtga acagcctgga cccccccctg 4680
ctgaccagat acctgaggat tcacccccag agctgggtgc accagattgc cctgaggatg 4740
gaggtgctgg gctgtgaggc ccaggacctg tactgaaata aaagatcttt attttcatta 4800
gatctgtgtg ttggtttttt gtgtgaggaa cccctagtga tggagttggc cactccctct 4860
ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt 4920
gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 4970
<210> 2
<211> 4950
<212> DNA
<213> 腺相关病毒
<400> 2
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactgtttg ctgcttgcaa tgtttgccca ttttagggtg gacacaggac 180
gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta gcccctgttt 240
gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc cccgttgccc 300
ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca gcttcaggca 360
ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc acctgcttct 420
tcctgtgcct gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg 480
agctgagctg ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc 540
cccccagagt gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt 600
ttgtggagtt cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc 660
tgctgggccc caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca 720
tggccagcca ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg 780
gggctgagta tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg 840
ggggcagcca cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc 900
ccctgtgcct gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg 960
gcctgattgg ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga 1020
ccctgcacaa gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg 1080
aaaccaagaa cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga 1140
tgcacactgt gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga 1200
agtctgtgta ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc 1260
tggagggcca caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca 1320
tcaccttcct gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc 1380
acatcagcag ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg 1440
aggagcccca gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga 1500
ctgactctga gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga 1560
tcaggtctgt ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg 1620
aggactggga ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt 1680
acctgaacaa tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct 1740
acactgatga aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc 1800
ccctgctgta tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca 1860
ggccctacaa catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc 1920
tgcccaaggg ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt 1980
acaagtggac tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca 2040
gatactacag cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc 2100
tgctgatctg ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga 2160
ggaatgtgat cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca 2220
tccagaggtt cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca 2280
gcaacatcat gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc 2340
tgcatgaggt ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg 2400
tgttcttctc tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt 2460
tccccttctc tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg 2520
gctgccacaa ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct 2580
gtgacaagaa cactggggac tactatgagg acagctatga ggacatctct gcctacctgc 2640
tgagcaagaa caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga 2700
ggcaccagag ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg 2760
atgacaccat ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga 2820
accagagccc caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga 2880
ggctgtggga ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg 2940
gctctgtgcc ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc 3000
agcccctgta cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg 3060
ctgaggtgga ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct 3120
tctacagcag cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga 3180
actttgtgaa gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc 3240
ccaccaagga tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga 3300
aggatgtgca ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc 3360
ctgcccatgg caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg 3420
aaaccaagag ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca 3480
tccagatgga ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca 3540
tcatggacac cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc 3600
tgagcatggg cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg 3660
tgaggaagaa ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga 3720
ctgtggagat gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc 3780
acctgcatgc tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc 3840
tgggcatggc ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc 3900
agtgggcccc caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca 3960
aggagccctt cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca 4020
agacccaggg ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt 4080
acagcctgga tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg 4140
tgttctttgg caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca 4200
ttgccagata catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg 4260
agctgatggg ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca 4320
tctctgatgc ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc 4380
ccagcaaggc caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca 4440
accccaagga gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca 4500
cccagggggt gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca 4560
gccaggatgg ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg 4620
gcaaccagga cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat 4680
acctgaggat tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg 4740
gctgtgaggc ccaggacctg tactgaaata aaagatcttt attttcatta gatctgtgtg 4800
ttggtttttt gtgtgagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 4860
tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 4920
cgagcgagcg cgcagagagg gagtggccaa 4950
<210> 3
<211> 4983
<212> DNA
<213> 腺相关病毒
<400> 3
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactgggcg actcagatcc cagccagtgg acttagcccc tgtttgctcc 180
tccgataact ggggtgacct tggttaatat tcaccagcag cctcccccgt tgcccctctg 240
gatccactgc ttaaatacgg acgaggacag ggccctgtct cctcagcttc aggcaccacc 300
actgacctgg gacagtgaat cgccaccatg cagattgagc tgagcacctg cttcttcctg 360
tgcctgctga gattctgctt tagtgccacc agaagatact acctgggtgc agtggaactg 420
tcatgggact atatgcaaag tgatctcggt gagctgcctg tggacgcaag gtaaatgccc 480
taaaatgggc aaacattgca agcagcaaac aacctggctc agaaaccaca gcgtcctgtg 540
tccattctaa tttttccttt cttcacgcag atttcctcct agagtgccaa aatcttttcc 600
attcaacacc tcagtcgtgt acaaaaagac tctgtttgta gaattcacgg atcacctttt 660
caacatcgct aagcccaggc ccccctggat gggcctgctg ggccccacca tccaggctga 720
ggtgtatgac actgtggtga tcaccctgaa gaacatggcc agccaccctg tgagcctgca 780
tgctgtgggg gtgagctact ggaaggcctc tgagggggct gagtatgatg accagaccag 840
ccagagggag aaggaggatg acaaggtgtt ccctgggggc agccacacct atgtgtggca 900
ggtgctgaag gagaatggcc ccatggcctc tgaccccctg tgcctgacct acagctacct 960
gagccatgtg gacctggtga aggacctgaa ctctggcctg attggggccc tgctggtgtg 1020
cagggagggc agcctggcca aggagaagac ccagaccctg cacaagttca tcctgctgtt 1080
tgctgtgttt gatgagggca agagctggca ctctgaaacc aagaacagcc tgatgcagga 1140
cagggatgct gcctctgcca gggcctggcc caagatgcac actgtgaatg gctatgtgaa 1200
caggagcctg cctggcctga ttggctgcca caggaagtct gtgtactggc atgtgattgg 1260
catgggcacc acccctgagg tgcacagcat cttcctggag ggccacacct tcctggtcag 1320
gaaccacagg caggccagcc tggagatcag ccccatcacc ttcctgactg cccagaccct 1380
gctgatggac ctgggccagt tcctgctgtt ctgccacatc agcagccacc agcatgatgg 1440
catggaggcc tatgtgaagg tggacagctg ccctgaggag ccccagctga ggatgaagaa 1500
caatgaggag gctgaggact atgatgatga cctgactgac tctgagatgg atgtggtgag 1560
gtttgatgat gacaacagcc ccagcttcat ccagatcagg tctgtggcca agaagcaccc 1620
caagacctgg gtgcactaca ttgctgctga ggaggaggac tgggactatg cccccctggt 1680
gctggcccct gatgacagga gctacaagag ccagtacctg aacaatggcc cccagaggat 1740
tggcaggaag tacaagaagg tcaggttcat ggcctacact gatgaaacct tcaagaccag 1800
ggaggccatc cagcatgagt ctggcatcct gggccccctg ctgtatgggg aggtggggga 1860
caccctgctg atcatcttca agaaccaggc cagcaggccc tacaacatct acccccatgg 1920
catcactgat gtgaggcccc tgtacagcag gaggctgccc aagggggtga agcacctgaa 1980
ggacttcccc atcctgcctg gggagatctt caagtacaag tggactgtga ctgtggagga 2040
tggccccacc aagtctgacc ccaggtgcct gaccagatac tacagcagct ttgtgaacat 2100
ggagagggac ctggcctctg gcctgattgg ccccctgctg atctgctaca aggagtctgt 2160
ggaccagagg ggcaaccaga tcatgtctga caagaggaat gtgatcctgt tctctgtgtt 2220
tgatgagaac aggagctggt acctgactga gaacatccag aggttcctgc ccaaccctgc 2280
tggggtgcag ctggaggacc ctgagttcca ggccagcaac atcatgcaca gcatcaatgg 2340
ctatgtgttt gacagcctgc agctgtctgt gtgcctgcat gaggtggcct actggtacat 2400
cctgagcatt ggggcccaga ctgacttcct gtctgtgttc ttctctggct acaccttcaa 2460
gcacaagatg gtgtatgagg acaccctgac cctgttcccc ttctctgggg agactgtgtt 2520
catgagcatg gagaaccctg gcctgtggat tctgggctgc cacaactctg acttcaggaa 2580
caggggcatg actgccctgc tgaaagtctc cagctgtgac aagaacactg gggactacta 2640
tgaggacagc tatgaggaca tctctgccta cctgctgagc aagaacaatg ccattgagcc 2700
caggagcttc agccagaacc ccccagtgct gaagaggcac cagagggaga tcaccaggac 2760
caccctgcag tctgaccagg aggagattga ctatgatgac accatctctg tggagatgaa 2820
gaaggaggac tttgacatct acgacgagga cgagaaccag agccccagga gcttccagaa 2880
gaagaccagg cactacttca ttgctgctgt ggagaggctg tgggactatg gcatgagcag 2940
cagcccccat gtgctgagga acagggccca gtctggctct gtgccccagt tcaagaaggt 3000
ggtgttccag gagttcactg atggcagctt cacccagccc ctgtacagag gggagctgaa 3060
tgagcacctg ggcctgctgg gcccctacat cagggctgag gtggaggaca acatcatggt 3120
gaccttcagg aaccaggcca gcaggcccta cagcttctac agcagcctga tcagctatga 3180
ggaggaccag aggcaggggg ctgagcccag gaagaacttt gtgaagccca atgaaaccaa 3240
gacctacttc tggaaggtgc agcaccacat ggcccccacc aaggatgagt ttgactgcaa 3300
ggcctgggcc tacttctctg atgtggacct ggagaaggat gtgcactctg gcctgattgg 3360
ccccctgctg gtgtgccaca ccaacaccct gaaccctgcc catggcaggc aggtgactgt 3420
gcaggagttt gccctgttct tcaccatctt tgatgaaacc aagagctggt acttcactga 3480
gaacatggag aggaactgca gggccccctg caacatccag atggaggacc ccaccttcaa 3540
ggagaactac aggttccatg ccatcaatgg ctacatcatg gacaccctgc ctggcctggt 3600
gatggcccag gaccagagga tcaggtggta cctgctgagc atgggcagca atgagaacat 3660
ccacagcatc cacttctctg gccatgtgtt cactgtgagg aagaaggagg agtacaagat 3720
ggccctgtac aacctgtacc ctggggtgtt tgagactgtg gagatgctgc ccagcaaggc 3780
tggcatctgg agggtggagt gcctgattgg ggagcacctg catgctggca tgagcaccct 3840
gttcctggtg tacagcaaca agtgccagac ccccctgggc atggcctctg gccacatcag 3900
ggacttccag atcactgcct ctggccagta tggccagtgg gcccccaagc tggccaggct 3960
gcactactct ggcagcatca atgcctggag caccaaggag cccttcagct ggatcaaggt 4020
ggacctgctg gcccccatga tcatccatgg catcaagacc cagggggcca ggcagaagtt 4080
cagcagcctg tacatcagcc agttcatcat catgtacagc ctggatggca agaagtggca 4140
gacctacagg ggcaacagca ctggcaccct gatggtgttc tttggcaatg tggacagctc 4200
tggcatcaag cacaacatct tcaacccccc catcattgcc agatacatca ggctgcaccc 4260
cacccactac agcatcagga gcaccctgag gatggagctg atgggctgtg acctgaacag 4320
ctgcagcatg cccctgggca tggagagcaa ggccatctct gatgcccaga tcactgccag 4380
cagctacttc accaacatgt ttgccacctg gagccccagc aaggccaggc tgcacctgca 4440
gggcaggagc aatgcctgga ggccccaggt caacaacccc aaggagtggc tgcaggtgga 4500
cttccagaag accatgaagg tgactggggt gaccacccag ggggtgaaga gcctgctgac 4560
cagcatgtat gtgaaggagt tcctgatcag cagcagccag gatggccacc agtggaccct 4620
gttcttccag aatggcaagg tgaaggtgtt ccagggcaac caggacagct tcacccctgt 4680
ggtgaacagc ctggaccccc ccctgctgac cagatacctg aggattcacc cccagagctg 4740
ggtgcaccag attgccctga ggatggaggt gctgggctgt gaggcccagg acctgtacta 4800
ataaaagatc tttattttca ttagatctgt gtgttggttt tttgtgtgag tgatggagtt 4860
ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg 4920
acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagag agggagtggc 4980
caa 4983
<210> 4
<211> 4984
<212> DNA
<213> 腺相关病毒
<400> 4
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactgggcg actcagatcc cagccagtgg acttagcccc tgtttgctcc 180
tccgataact ggggtgacct tggttaatat tcaccagcag cctcccccgt tgcccctctg 240
gatccactgc ttaaatacgg acgaggacag ggccctgtct cctcagcttc aggcaccacc 300
actgacctgg gacagtgaat cgccaccatg cagattgagc tgagcacctg cttcttcctg 360
tgcctgctga gattctgctt tagtgccacc agaagatact acctgggtgc agtggaactg 420
tcatgggact atatgcaaag tgatctcggt gagctgcctg tggacgcaag gtaaatgccc 480
taaaatgggc aaacattgca agcagcaaac accctaaaat gggcaaacat tgcaagcagc 540
aaacattcta atttttcctt tcttcacgca gatttcctcc tagagtgcca aaatcttttc 600
cattcaacac ctcagtcgtg tacaaaaaga ctctgtttgt agaattcacg gatcaccttt 660
tcaacatcgc taagcccagg cccccctgga tgggcctgct gggccccacc atccaggctg 720
aggtgtatga cactgtggtg atcaccctga agaacatggc cagccaccct gtgagcctgc 780
atgctgtggg ggtgagctac tggaaggcct ctgagggggc tgagtatgat gaccagacca 840
gccagaggga gaaggaggat gacaaggtgt tccctggggg cagccacacc tatgtgtggc 900
aggtgctgaa ggagaatggc cccatggcct ctgaccccct gtgcctgacc tacagctacc 960
tgagccatgt ggacctggtg aaggacctga actctggcct gattggggcc ctgctggtgt 1020
gcagggaggg cagcctggcc aaggagaaga cccagaccct gcacaagttc atcctgctgt 1080
ttgctgtgtt tgatgagggc aagagctggc actctgaaac caagaacagc ctgatgcagg 1140
acagggatgc tgcctctgcc agggcctggc ccaagatgca cactgtgaat ggctatgtga 1200
acaggagcct gcctggcctg attggctgcc acaggaagtc tgtgtactgg catgtgattg 1260
gcatgggcac cacccctgag gtgcacagca tcttcctgga gggccacacc ttcctggtca 1320
ggaaccacag gcaggccagc ctggagatca gccccatcac cttcctgact gcccagaccc 1380
tgctgatgga cctgggccag ttcctgctgt tctgccacat cagcagccac cagcatgatg 1440
gcatggaggc ctatgtgaag gtggacagct gccctgagga gccccagctg aggatgaaga 1500
acaatgagga ggctgaggac tatgatgatg acctgactga ctctgagatg gatgtggtga 1560
ggtttgatga tgacaacagc cccagcttca tccagatcag gtctgtggcc aagaagcacc 1620
ccaagacctg ggtgcactac attgctgctg aggaggagga ctgggactat gcccccctgg 1680
tgctggcccc tgatgacagg agctacaaga gccagtacct gaacaatggc ccccagagga 1740
ttggcaggaa gtacaagaag gtcaggttca tggcctacac tgatgaaacc ttcaagacca 1800
gggaggccat ccagcatgag tctggcatcc tgggccccct gctgtatggg gaggtggggg 1860
acaccctgct gatcatcttc aagaaccagg ccagcaggcc ctacaacatc tacccccatg 1920
gcatcactga tgtgaggccc ctgtacagca ggaggctgcc caagggggtg aagcacctga 1980
aggacttccc catcctgcct ggggagatct tcaagtacaa gtggactgtg actgtggagg 2040
atggccccac caagtctgac cccaggtgcc tgaccagata ctacagcagc tttgtgaaca 2100
tggagaggga cctggcctct ggcctgattg gccccctgct gatctgctac aaggagtctg 2160
tggaccagag gggcaaccag atcatgtctg acaagaggaa tgtgatcctg ttctctgtgt 2220
ttgatgagaa caggagctgg tacctgactg agaacatcca gaggttcctg cccaaccctg 2280
ctggggtgca gctggaggac cctgagttcc aggccagcaa catcatgcac agcatcaatg 2340
gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc tactggtaca 2400
tcctgagcat tggggcccag actgacttcc tgtctgtgtt cttctctggc tacaccttca 2460
agcacaagat ggtgtatgag gacaccctga ccctgttccc cttctctggg gagactgtgt 2520
tcatgagcat ggagaaccct ggcctgtgga ttctgggctg ccacaactct gacttcagga 2580
acaggggcat gactgccctg ctgaaagtct ccagctgtga caagaacact ggggactact 2640
atgaggacag ctatgaggac atctctgcct acctgctgag caagaacaat gccattgagc 2700
ccaggagctt cagccagaac cccccagtgc tgaagaggca ccagagggag atcaccagga 2760
ccaccctgca gtctgaccag gaggagattg actatgatga caccatctct gtggagatga 2820
agaaggagga ctttgacatc tacgacgagg acgagaacca gagccccagg agcttccaga 2880
agaagaccag gcactacttc attgctgctg tggagaggct gtgggactat ggcatgagca 2940
gcagccccca tgtgctgagg aacagggccc agtctggctc tgtgccccag ttcaagaagg 3000
tggtgttcca ggagttcact gatggcagct tcacccagcc cctgtacaga ggggagctga 3060
atgagcacct gggcctgctg ggcccctaca tcagggctga ggtggaggac aacatcatgg 3120
tgaccttcag gaaccaggcc agcaggccct acagcttcta cagcagcctg atcagctatg 3180
aggaggacca gaggcagggg gctgagccca ggaagaactt tgtgaagccc aatgaaacca 3240
agacctactt ctggaaggtg cagcaccaca tggcccccac caaggatgag tttgactgca 3300
aggcctgggc ctacttctct gatgtggacc tggagaagga tgtgcactct ggcctgattg 3360
gccccctgct ggtgtgccac accaacaccc tgaaccctgc ccatggcagg caggtgactg 3420
tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac caagagctgg tacttcactg 3480
agaacatgga gaggaactgc agggccccct gcaacatcca gatggaggac cccaccttca 3540
aggagaacta caggttccat gccatcaatg gctacatcat ggacaccctg cctggcctgg 3600
tgatggccca ggaccagagg atcaggtggt acctgctgag catgggcagc aatgagaaca 3660
tccacagcat ccacttctct ggccatgtgt tcactgtgag gaagaaggag gagtacaaga 3720
tggccctgta caacctgtac cctggggtgt ttgagactgt ggagatgctg cccagcaagg 3780
ctggcatctg gagggtggag tgcctgattg gggagcacct gcatgctggc atgagcaccc 3840
tgttcctggt gtacagcaac aagtgccaga cccccctggg catggcctct ggccacatca 3900
gggacttcca gatcactgcc tctggccagt atggccagtg ggcccccaag ctggccaggc 3960
tgcactactc tggcagcatc aatgcctgga gcaccaagga gcccttcagc tggatcaagg 4020
tggacctgct ggcccccatg atcatccatg gcatcaagac ccagggggcc aggcagaagt 4080
tcagcagcct gtacatcagc cagttcatca tcatgtacag cctggatggc aagaagtggc 4140
agacctacag gggcaacagc actggcaccc tgatggtgtt ctttggcaat gtggacagct 4200
ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc cagatacatc aggctgcacc 4260
ccacccacta cagcatcagg agcaccctga ggatggagct gatgggctgt gacctgaaca 4320
gctgcagcat gcccctgggc atggagagca aggccatctc tgatgcccag atcactgcca 4380
gcagctactt caccaacatg tttgccacct ggagccccag caaggccagg ctgcacctgc 4440
agggcaggag caatgcctgg aggccccagg tcaacaaccc caaggagtgg ctgcaggtgg 4500
acttccagaa gaccatgaag gtgactgggg tgaccaccca gggggtgaag agcctgctga 4560
ccagcatgta tgtgaaggag ttcctgatca gcagcagcca ggatggccac cagtggaccc 4620
tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa ccaggacagc ttcacccctg 4680
tggtgaacag cctggacccc cccctgctga ccagatacct gaggattcac ccccagagct 4740
gggtgcacca gattgccctg aggatggagg tgctgggctg tgaggcccag gacctgtact 4800
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtga gtgatggagt 4860
tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 4920
gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg 4980
ccaa 4984
<210> 5
<211> 4805
<212> DNA
<213> 腺相关病毒
<400> 5
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgtttg ctgcttgcaa tgtttgccca ttttagggtg 180
gacacaggac gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta 240
gcccctgttt gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc 300
cccgttgccc ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca 360
gcttcaggca ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc 420
acctgcttct tcctgtgcct gctgaggttc tgcttctctg ccaccaggag atactacctg 480
ggggctgtgg agctgagctg ggactacatg cagtctgacc tgggggagct gcctgtggat 540
gccaggttcc cccccagagt gcccaagagc ttccccttca acacctctgt ggtgtacaag 600
aagaccctgt ttgtggagtt cactgaccac ctgttcaaca ttgccaagcc caggcccccc 660
tggatgggcc tgctgggccc caccatccag gctgaggtgt atgacactgt ggtgatcacc 720
ctgaagaaca tggccagcca ccctgtgagc ctgcatgctg tgggggtgag ctactggaag 780
gcctctgagg gggctgagta tgatgaccag accagccaga gggagaagga ggatgacaag 840
gtgttccctg ggggcagcca cacctatgtg tggcaggtgc tgaaggagaa tggccccatg 900
gcctctgacc ccctgtgcct gacctacagc tacctgagcc atgtggacct ggtgaaggac 960
ctgaactctg gcctgattgg ggccctgctg gtgtgcaggg agggcagcct ggccaaggag 1020
aagacccaga ccctgcacaa gttcatcctg ctgtttgctg tgtttgatga gggcaagagc 1080
tggcactctg aaaccaagaa cagcctgatg caggacaggg atgctgcctc tgccagggcc 1140
tggcccaaga tgcacactgt gaatggctat gtgaacagga gcctgcctgg cctgattggc 1200
tgccacagga agtctgtgta ctggcatgtg attggcatgg gcaccacccc tgaggtgcac 1260
agcatcttcc tggagggcca caccttcctg gtcaggaacc acaggcaggc cagcctggag 1320
atcagcccca tcaccttcct gactgcccag accctgctga tggacctggg ccagttcctg 1380
ctgttctgcc acatcagcag ccaccagcat gatggcatgg aggcctatgt gaaggtggac 1440
agctgccctg aggagcccca gctgaggatg aagaacaatg aggaggctga ggactatgat 1500
gatgacctga ctgactctga gatggatgtg gtgaggtttg atgatgacaa cagccccagc 1560
ttcatccaga tcaggtctgt ggccaagaag caccccaaga cctgggtgca ctacattgct 1620
gctgaggagg aggactggga ctatgccccc ctggtgctgg cccctgatga caggagctac 1680
aagagccagt acctgaacaa tggcccccag aggattggca ggaagtacaa gaaggtcagg 1740
ttcatggcct acactgatga aaccttcaag accagggagg ccatccagca tgagtctggc 1800
atcctgggcc ccctgctgta tggggaggtg ggggacaccc tgctgatcat cttcaagaac 1860
caggccagca ggccctacaa catctacccc catggcatca ctgatgtgag gcccctgtac 1920
agcaggaggc tgcccaaggg ggtgaagcac ctgaaggact tccccatcct gcctggggag 1980
atcttcaagt acaagtggac tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg 2040
tgcctgacca gatactacag cagctttgtg aacatggaga gggacctggc ctctggcctg 2100
attggccccc tgctgatctg ctacaaggag tctgtggacc agaggggcaa ccagatcatg 2160
tctgacaaga ggaatgtgat cctgttctct gtgtttgatg agaacaggag ctggtacctg 2220
actgagaaca tccagaggtt cctgcccaac cctgctgggg tgcagctgga ggaccctgag 2280
ttccaggcca gcaacatcat gcacagcatc aatggctatg tgtttgacag cctgcagctg 2340
tctgtgtgcc tgcatgaggt ggcctactgg tacatcctga gcattggggc ccagactgac 2400
ttcctgtctg tgttcttctc tggctacacc ttcaagcaca agatggtgta tgaggacacc 2460
ctgaccctgt tccccttctc tggggagact gtgttcatga gcatggagaa ccctggcctg 2520
tggattctgg gctgccacaa ctctgacttc aggaacaggg gcatgactgc cctgctgaaa 2580
gtctccagct gtgacaagaa cactggggac tactatgagg acagctatga ggacatctct 2640
gcctacctgc tgagcaagaa caatgccatt gagcccagga gcttccagaa gaagaccagg 2700
cactacttca ttgctgctgt ggagaggctg tgggactatg gcatgagcag cagcccccat 2760
gtgctgagga acagggccca gtctggctct gtgccccagt tcaagaaggt ggtgttccag 2820
gagttcactg atggcagctt cacccagccc ctgtacagag gggagctgaa tgagcacctg 2880
ggcctgctgg gcccctacat cagggctgag gtggaggaca acatcatggt gaccttcagg 2940
aaccaggcca gcaggcccta cagcttctac agcagcctga tcagctatga ggaggaccag 3000
aggcaggggg ctgagcccag gaagaacttt gtgaagccca atgaaaccaa gacctacttc 3060
tggaaggtgc agcaccacat ggcccccacc aaggatgagt ttgactgcaa ggcctgggcc 3120
tacttctctg atgtggacct ggagaaggat gtgcactctg gcctgattgg ccccctgctg 3180
gtgtgccaca ccaacaccct gaaccctgcc catggcaggc aggtgactgt gcaggagttt 3240
gccctgttct tcaccatctt tgatgaaacc aagagctggt acttcactga gaacatggag 3300
aggaactgca gggccccctg caacatccag atggaggacc ccaccttcaa ggagaactac 3360
aggttccatg ccatcaatgg ctacatcatg gacaccctgc ctggcctggt gatggcccag 3420
gaccagagga tcaggtggta cctgctgagc atgggcagca atgagaacat ccacagcatc 3480
cacttctctg gccatgtgtt cactgtgagg aagaaggagg agtacaagat ggccctgtac 3540
aacctgtacc ctggggtgtt tgagactgtg gagatgctgc ccagcaaggc tggcatctgg 3600
agggtggagt gcctgattgg ggagcacctg catgctggca tgagcaccct gttcctggtg 3660
tacagcaaca agtgccagac ccccctgggc atggcctctg gccacatcag ggacttccag 3720
atcactgcct ctggccagta tggccagtgg gcccccaagc tggccaggct gcactactct 3780
ggcagcatca atgcctggag caccaaggag cccttcagct ggatcaaggt ggacctgctg 3840
gcccccatga tcatccatgg catcaagacc cagggggcca ggcagaagtt cagcagcctg 3900
tacatcagcc agttcatcat catgtacagc ctggatggca agaagtggca gacctacagg 3960
ggcaacagca ctggcaccct gatggtgttc tttggcaatg tggacagctc tggcatcaag 4020
cacaacatct tcaacccccc catcattgcc agatacatca ggctgcaccc cacccactac 4080
agcatcagga gcaccctgag gatggagctg atgggctgtg acctgaacag ctgcagcatg 4140
cccctgggca tggagagcaa ggccatctct gatgcccaga tcactgccag cagctacttc 4200
accaacatgt ttgccacctg gagccccagc aaggccaggc tgcacctgca gggcaggagc 4260
aatgcctgga ggccccaggt caacaacccc aaggagtggc tgcaggtgga cttccagaag 4320
accatgaagg tgactggggt gaccacccag ggggtgaaga gcctgctgac cagcatgtat 4380
gtgaaggagt tcctgatcag cagcagccag gatggccacc agtggaccct gttcttccag 4440
aatggcaagg tgaaggtgtt ccagggcaac caggacagct tcacccctgt ggtgaacagc 4500
ctggaccccc ccctgctgac cagatacctg aggattcacc cccagagctg ggtgcaccag 4560
attgccctga ggatggaggt gctgggctgt gaggcccagg acctgtactg aaataaaaga 4620
tctttatttt cattagatct gtgtgttggt tttttgtgtg aggaacccct agtgatggag 4680
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc 4740
cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg 4800
gccaa 4805
<210> 6
<211> 4934
<212> DNA
<213> 腺相关病毒
<400> 6
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgtttg ctgcttgcaa tgtttgccca ttttagggtg 180
gacacaggac gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta 240
gcccctgttt gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc 300
cccgttgccc ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca 360
gcttcaggca ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc 420
acctgcttct tcctgtgcct gctgagattc tgctttagtg ccaccagaag atactacctg 480
ggtgcagtgg aactgtcatg ggactatatg caaagtgatc tcggtgagct gcctgtggac 540
gcaaggtaaa ggcatgtcct gtagggtctg atcggggcca ggattgtggg gatgtaagtc 600
tgcttggagg aaggtgcaga catcgggtta ggatggttgt gatgctattc tgactttttc 660
ctttcttcac gcagatttcc tcctagagtg ccaaaatctt ttccattcaa cacctcagtc 720
gtgtacaaaa agactctgtt tgtagaattc acggatcacc ttttcaacat cgctaagccc 780
aggcccccct ggatgggcct gctgggcccc accatccagg ctgaggtgta tgacactgtg 840
gtgatcaccc tgaagaacat ggccagccac cctgtgagcc tgcatgctgt gggggtgagc 900
tactggaagg cctctgaggg ggctgagtat gatgaccaga ccagccagag ggagaaggag 960
gatgacaagg tgttccctgg gggcagccac acctatgtgt ggcaggtgct gaaggagaat 1020
ggccccatgg cctctgaccc cctgtgcctg acctacagct acctgagcca tgtggacctg 1080
gtgaaggacc tgaactctgg cctgattggg gccctgctgg tgtgcaggga gggcagcctg 1140
gccaaggaga agacccagac cctgcacaag ttcatcctgc tgtttgctgt gtttgatgag 1200
ggcaagagct ggcactctga aaccaagaac agcctgatgc aggacaggga tgctgcctct 1260
gccagggcct ggcccaagat gcacactgtg aatggctatg tgaacaggag cctgcctggc 1320
ctgattggct gccacaggaa gtctgtgtac tggcatgtga ttggcatggg caccacccct 1380
gaggtgcaca gcatcttcct ggagggccac accttcctgg tcaggaacca caggcaggcc 1440
agcctggaga tcagccccat caccttcctg actgcccaga ccctgctgat ggacctgggc 1500
cagttcctgc tgttctgcca catcagcagc caccagcatg atggcatgga ggcctatgtg 1560
aaggtggaca gctgccctga ggagccccag ctgaggatga agaacaatga ggaggctgag 1620
gactatgatg atgacctgac tgactctgag atggatgtgg tgaggtttga tgatgacaac 1680
agccccagct tcatccagat caggtctgtg gccaagaagc accccaagac ctgggtgcac 1740
tacattgctg ctgaggagga ggactgggac tatgcccccc tggtgctggc ccctgatgac 1800
aggagctaca agagccagta cctgaacaat ggcccccaga ggattggcag gaagtacaag 1860
aaggtcaggt tcatggccta cactgatgaa accttcaaga ccagggaggc catccagcat 1920
gagtctggca tcctgggccc cctgctgtat ggggaggtgg gggacaccct gctgatcatc 1980
ttcaagaacc aggccagcag gccctacaac atctaccccc atggcatcac tgatgtgagg 2040
cccctgtaca gcaggaggct gcccaagggg gtgaagcacc tgaaggactt ccccatcctg 2100
cctggggaga tcttcaagta caagtggact gtgactgtgg aggatggccc caccaagtct 2160
gaccccaggt gcctgaccag atactacagc agctttgtga acatggagag ggacctggcc 2220
tctggcctga ttggccccct gctgatctgc tacaaggagt ctgtggacca gaggggcaac 2280
cagatcatgt ctgacaagag gaatgtgatc ctgttctctg tgtttgatga gaacaggagc 2340
tggtacctga ctgagaacat ccagaggttc ctgcccaacc ctgctggggt gcagctggag 2400
gaccctgagt tccaggccag caacatcatg cacagcatca atggctatgt gtttgacagc 2460
ctgcagctgt ctgtgtgcct gcatgaggtg gcctactggt acatcctgag cattggggcc 2520
cagactgact tcctgtctgt gttcttctct ggctacacct tcaagcacaa gatggtgtat 2580
gaggacaccc tgaccctgtt ccccttctct ggggagactg tgttcatgag catggagaac 2640
cctggcctgt ggattctggg ctgccacaac tctgacttca ggaacagggg catgactgcc 2700
ctgctgaaag tctccagctg tgacaagaac actggggact actatgagga cagctatgag 2760
gacatctctg cctacctgct gagcaagaac aatgccattg agcccaggag cttccagaag 2820
aagaccaggc actacttcat tgctgctgtg gagaggctgt gggactatgg catgagcagc 2880
agcccccatg tgctgaggaa cagggcccag tctggctctg tgccccagtt caagaaggtg 2940
gtgttccagg agttcactga tggcagcttc acccagcccc tgtacagagg ggagctgaat 3000
gagcacctgg gcctgctggg cccctacatc agggctgagg tggaggacaa catcatggtg 3060
accttcagga accaggccag caggccctac agcttctaca gcagcctgat cagctatgag 3120
gaggaccaga ggcagggggc tgagcccagg aagaactttg tgaagcccaa tgaaaccaag 3180
acctacttct ggaaggtgca gcaccacatg gcccccacca aggatgagtt tgactgcaag 3240
gcctgggcct acttctctga tgtggacctg gagaaggatg tgcactctgg cctgattggc 3300
cccctgctgg tgtgccacac caacaccctg aaccctgccc atggcaggca ggtgactgtg 3360
caggagtttg ccctgttctt caccatcttt gatgaaacca agagctggta cttcactgag 3420
aacatggaga ggaactgcag ggccccctgc aacatccaga tggaggaccc caccttcaag 3480
gagaactaca ggttccatgc catcaatggc tacatcatgg acaccctgcc tggcctggtg 3540
atggcccagg accagaggat caggtggtac ctgctgagca tgggcagcaa tgagaacatc 3600
cacagcatcc acttctctgg ccatgtgttc actgtgagga agaaggagga gtacaagatg 3660
gccctgtaca acctgtaccc tggggtgttt gagactgtgg agatgctgcc cagcaaggct 3720
ggcatctgga gggtggagtg cctgattggg gagcacctgc atgctggcat gagcaccctg 3780
ttcctggtgt acagcaacaa gtgccagacc cccctgggca tggcctctgg ccacatcagg 3840
gacttccaga tcactgcctc tggccagtat ggccagtggg cccccaagct ggccaggctg 3900
cactactctg gcagcatcaa tgcctggagc accaaggagc ccttcagctg gatcaaggtg 3960
gacctgctgg cccccatgat catccatggc atcaagaccc agggggccag gcagaagttc 4020
agcagcctgt acatcagcca gttcatcatc atgtacagcc tggatggcaa gaagtggcag 4080
acctacaggg gcaacagcac tggcaccctg atggtgttct ttggcaatgt ggacagctct 4140
ggcatcaagc acaacatctt caaccccccc atcattgcca gatacatcag gctgcacccc 4200
acccactaca gcatcaggag caccctgagg atggagctga tgggctgtga cctgaacagc 4260
tgcagcatgc ccctgggcat ggagagcaag gccatctctg atgcccagat cactgccagc 4320
agctacttca ccaacatgtt tgccacctgg agccccagca aggccaggct gcacctgcag 4380
ggcaggagca atgcctggag gccccaggtc aacaacccca aggagtggct gcaggtggac 4440
ttccagaaga ccatgaaggt gactggggtg accacccagg gggtgaagag cctgctgacc 4500
agcatgtatg tgaaggagtt cctgatcagc agcagccagg atggccacca gtggaccctg 4560
ttcttccaga atggcaaggt gaaggtgttc cagggcaacc aggacagctt cacccctgtg 4620
gtgaacagcc tggacccccc cctgctgacc agatacctga ggattcaccc ccagagctgg 4680
gtgcaccaga ttgccctgag gatggaggtg ctgggctgtg aggcccagga cctgtactga 4740
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtga ggaaccccta 4800
gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 4860
aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga 4920
gagggagtgg ccaa 4934
<210> 7
<211> 4934
<212> DNA
<213> 腺相关病毒
<400> 7
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgtttg ctgcttgcaa tgtttgccca ttttagggtg 180
gacacaggac gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta 240
gcccctgttt gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc 300
cccgttgccc ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca 360
gcttcaggca ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc 420
acctgcttct tcctgtgcct gctgagattc tgctttagtg ccaccagaag atactacctg 480
ggtgcagtgg aactgtcatg ggactatatg caaagtgatc tcggtgagct gcctgtggac 540
gcaaggtaaa ggctgtttgc tgcttgcaat gtttgcccat tttagggggg gatgtaagtc 600
tgcttggagg aaggtgcaga catcgggtta ggatggttgt gatgctattc tgactttttc 660
ctttcttcac gcagatttcc tcctagagtg ccaaaatctt ttccattcaa cacctcagtc 720
gtgtacaaaa agactctgtt tgtagaattc acggatcacc ttttcaacat cgctaagccc 780
aggcccccct ggatgggcct gctgggcccc accatccagg ctgaggtgta tgacactgtg 840
gtgatcaccc tgaagaacat ggccagccac cctgtgagcc tgcatgctgt gggggtgagc 900
tactggaagg cctctgaggg ggctgagtat gatgaccaga ccagccagag ggagaaggag 960
gatgacaagg tgttccctgg gggcagccac acctatgtgt ggcaggtgct gaaggagaat 1020
ggccccatgg cctctgaccc cctgtgcctg acctacagct acctgagcca tgtggacctg 1080
gtgaaggacc tgaactctgg cctgattggg gccctgctgg tgtgcaggga gggcagcctg 1140
gccaaggaga agacccagac cctgcacaag ttcatcctgc tgtttgctgt gtttgatgag 1200
ggcaagagct ggcactctga aaccaagaac agcctgatgc aggacaggga tgctgcctct 1260
gccagggcct ggcccaagat gcacactgtg aatggctatg tgaacaggag cctgcctggc 1320
ctgattggct gccacaggaa gtctgtgtac tggcatgtga ttggcatggg caccacccct 1380
gaggtgcaca gcatcttcct ggagggccac accttcctgg tcaggaacca caggcaggcc 1440
agcctggaga tcagccccat caccttcctg actgcccaga ccctgctgat ggacctgggc 1500
cagttcctgc tgttctgcca catcagcagc caccagcatg atggcatgga ggcctatgtg 1560
aaggtggaca gctgccctga ggagccccag ctgaggatga agaacaatga ggaggctgag 1620
gactatgatg atgacctgac tgactctgag atggatgtgg tgaggtttga tgatgacaac 1680
agccccagct tcatccagat caggtctgtg gccaagaagc accccaagac ctgggtgcac 1740
tacattgctg ctgaggagga ggactgggac tatgcccccc tggtgctggc ccctgatgac 1800
aggagctaca agagccagta cctgaacaat ggcccccaga ggattggcag gaagtacaag 1860
aaggtcaggt tcatggccta cactgatgaa accttcaaga ccagggaggc catccagcat 1920
gagtctggca tcctgggccc cctgctgtat ggggaggtgg gggacaccct gctgatcatc 1980
ttcaagaacc aggccagcag gccctacaac atctaccccc atggcatcac tgatgtgagg 2040
cccctgtaca gcaggaggct gcccaagggg gtgaagcacc tgaaggactt ccccatcctg 2100
cctggggaga tcttcaagta caagtggact gtgactgtgg aggatggccc caccaagtct 2160
gaccccaggt gcctgaccag atactacagc agctttgtga acatggagag ggacctggcc 2220
tctggcctga ttggccccct gctgatctgc tacaaggagt ctgtggacca gaggggcaac 2280
cagatcatgt ctgacaagag gaatgtgatc ctgttctctg tgtttgatga gaacaggagc 2340
tggtacctga ctgagaacat ccagaggttc ctgcccaacc ctgctggggt gcagctggag 2400
gaccctgagt tccaggccag caacatcatg cacagcatca atggctatgt gtttgacagc 2460
ctgcagctgt ctgtgtgcct gcatgaggtg gcctactggt acatcctgag cattggggcc 2520
cagactgact tcctgtctgt gttcttctct ggctacacct tcaagcacaa gatggtgtat 2580
gaggacaccc tgaccctgtt ccccttctct ggggagactg tgttcatgag catggagaac 2640
cctggcctgt ggattctggg ctgccacaac tctgacttca ggaacagggg catgactgcc 2700
ctgctgaaag tctccagctg tgacaagaac actggggact actatgagga cagctatgag 2760
gacatctctg cctacctgct gagcaagaac aatgccattg agcccaggag cttccagaag 2820
aagaccaggc actacttcat tgctgctgtg gagaggctgt gggactatgg catgagcagc 2880
agcccccatg tgctgaggaa cagggcccag tctggctctg tgccccagtt caagaaggtg 2940
gtgttccagg agttcactga tggcagcttc acccagcccc tgtacagagg ggagctgaat 3000
gagcacctgg gcctgctggg cccctacatc agggctgagg tggaggacaa catcatggtg 3060
accttcagga accaggccag caggccctac agcttctaca gcagcctgat cagctatgag 3120
gaggaccaga ggcagggggc tgagcccagg aagaactttg tgaagcccaa tgaaaccaag 3180
acctacttct ggaaggtgca gcaccacatg gcccccacca aggatgagtt tgactgcaag 3240
gcctgggcct acttctctga tgtggacctg gagaaggatg tgcactctgg cctgattggc 3300
cccctgctgg tgtgccacac caacaccctg aaccctgccc atggcaggca ggtgactgtg 3360
caggagtttg ccctgttctt caccatcttt gatgaaacca agagctggta cttcactgag 3420
aacatggaga ggaactgcag ggccccctgc aacatccaga tggaggaccc caccttcaag 3480
gagaactaca ggttccatgc catcaatggc tacatcatgg acaccctgcc tggcctggtg 3540
atggcccagg accagaggat caggtggtac ctgctgagca tgggcagcaa tgagaacatc 3600
cacagcatcc acttctctgg ccatgtgttc actgtgagga agaaggagga gtacaagatg 3660
gccctgtaca acctgtaccc tggggtgttt gagactgtgg agatgctgcc cagcaaggct 3720
ggcatctgga gggtggagtg cctgattggg gagcacctgc atgctggcat gagcaccctg 3780
ttcctggtgt acagcaacaa gtgccagacc cccctgggca tggcctctgg ccacatcagg 3840
gacttccaga tcactgcctc tggccagtat ggccagtggg cccccaagct ggccaggctg 3900
cactactctg gcagcatcaa tgcctggagc accaaggagc ccttcagctg gatcaaggtg 3960
gacctgctgg cccccatgat catccatggc atcaagaccc agggggccag gcagaagttc 4020
agcagcctgt acatcagcca gttcatcatc atgtacagcc tggatggcaa gaagtggcag 4080
acctacaggg gcaacagcac tggcaccctg atggtgttct ttggcaatgt ggacagctct 4140
ggcatcaagc acaacatctt caaccccccc atcattgcca gatacatcag gctgcacccc 4200
acccactaca gcatcaggag caccctgagg atggagctga tgggctgtga cctgaacagc 4260
tgcagcatgc ccctgggcat ggagagcaag gccatctctg atgcccagat cactgccagc 4320
agctacttca ccaacatgtt tgccacctgg agccccagca aggccaggct gcacctgcag 4380
ggcaggagca atgcctggag gccccaggtc aacaacccca aggagtggct gcaggtggac 4440
ttccagaaga ccatgaaggt gactggggtg accacccagg gggtgaagag cctgctgacc 4500
agcatgtatg tgaaggagtt cctgatcagc agcagccagg atggccacca gtggaccctg 4560
ttcttccaga atggcaaggt gaaggtgttc cagggcaacc aggacagctt cacccctgtg 4620
gtgaacagcc tggacccccc cctgctgacc agatacctga ggattcaccc ccagagctgg 4680
gtgcaccaga ttgccctgag gatggaggtg ctgggctgtg aggcccagga cctgtactga 4740
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtga ggaaccccta 4800
gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 4860
aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga 4920
gagggagtgg ccaa 4934
<210> 8
<211> 4934
<212> DNA
<213> 腺相关病毒
<400> 8
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgtttg ctgcttgcaa tgtttgccca ttttagggtg 180
gacacaggac gctgtggttt ctgagccagg gggcgactca gatcccagcc agtggactta 240
gcccctgttt gctcctccga taactggggt gaccttggtt aatattcacc agcagcctcc 300
cccgttgccc ctctggatcc actgcttaaa tacggacgag gacagggccc tgtctcctca 360
gcttcaggca ccaccactga cctgggacag tgaatcgcca ccatgcagat tgagctgagc 420
acctgcttct tcctgtgcct gctgagattc tgctttagtg ccaccagaag atactacctg 480
ggtgcagtgg aactgtcatg ggactatatg caaagtgatc tcggtgagct gcctgtggac 540
gcaaggtaaa ggcatgtcct gtagggtctg atcggggcca ggattgtggg gatgtaagtc 600
tgcttggagg aagccctaaa atgggcaaac attgcaagca gcaaacattc tgactttttc 660
ctttcttcac gcagatttcc tcctagagtg ccaaaatctt ttccattcaa cacctcagtc 720
gtgtacaaaa agactctgtt tgtagaattc acggatcacc ttttcaacat cgctaagccc 780
aggcccccct ggatgggcct gctgggcccc accatccagg ctgaggtgta tgacactgtg 840
gtgatcaccc tgaagaacat ggccagccac cctgtgagcc tgcatgctgt gggggtgagc 900
tactggaagg cctctgaggg ggctgagtat gatgaccaga ccagccagag ggagaaggag 960
gatgacaagg tgttccctgg gggcagccac acctatgtgt ggcaggtgct gaaggagaat 1020
ggccccatgg cctctgaccc cctgtgcctg acctacagct acctgagcca tgtggacctg 1080
gtgaaggacc tgaactctgg cctgattggg gccctgctgg tgtgcaggga gggcagcctg 1140
gccaaggaga agacccagac cctgcacaag ttcatcctgc tgtttgctgt gtttgatgag 1200
ggcaagagct ggcactctga aaccaagaac agcctgatgc aggacaggga tgctgcctct 1260
gccagggcct ggcccaagat gcacactgtg aatggctatg tgaacaggag cctgcctggc 1320
ctgattggct gccacaggaa gtctgtgtac tggcatgtga ttggcatggg caccacccct 1380
gaggtgcaca gcatcttcct ggagggccac accttcctgg tcaggaacca caggcaggcc 1440
agcctggaga tcagccccat caccttcctg actgcccaga ccctgctgat ggacctgggc 1500
cagttcctgc tgttctgcca catcagcagc caccagcatg atggcatgga ggcctatgtg 1560
aaggtggaca gctgccctga ggagccccag ctgaggatga agaacaatga ggaggctgag 1620
gactatgatg atgacctgac tgactctgag atggatgtgg tgaggtttga tgatgacaac 1680
agccccagct tcatccagat caggtctgtg gccaagaagc accccaagac ctgggtgcac 1740
tacattgctg ctgaggagga ggactgggac tatgcccccc tggtgctggc ccctgatgac 1800
aggagctaca agagccagta cctgaacaat ggcccccaga ggattggcag gaagtacaag 1860
aaggtcaggt tcatggccta cactgatgaa accttcaaga ccagggaggc catccagcat 1920
gagtctggca tcctgggccc cctgctgtat ggggaggtgg gggacaccct gctgatcatc 1980
ttcaagaacc aggccagcag gccctacaac atctaccccc atggcatcac tgatgtgagg 2040
cccctgtaca gcaggaggct gcccaagggg gtgaagcacc tgaaggactt ccccatcctg 2100
cctggggaga tcttcaagta caagtggact gtgactgtgg aggatggccc caccaagtct 2160
gaccccaggt gcctgaccag atactacagc agctttgtga acatggagag ggacctggcc 2220
tctggcctga ttggccccct gctgatctgc tacaaggagt ctgtggacca gaggggcaac 2280
cagatcatgt ctgacaagag gaatgtgatc ctgttctctg tgtttgatga gaacaggagc 2340
tggtacctga ctgagaacat ccagaggttc ctgcccaacc ctgctggggt gcagctggag 2400
gaccctgagt tccaggccag caacatcatg cacagcatca atggctatgt gtttgacagc 2460
ctgcagctgt ctgtgtgcct gcatgaggtg gcctactggt acatcctgag cattggggcc 2520
cagactgact tcctgtctgt gttcttctct ggctacacct tcaagcacaa gatggtgtat 2580
gaggacaccc tgaccctgtt ccccttctct ggggagactg tgttcatgag catggagaac 2640
cctggcctgt ggattctggg ctgccacaac tctgacttca ggaacagggg catgactgcc 2700
ctgctgaaag tctccagctg tgacaagaac actggggact actatgagga cagctatgag 2760
gacatctctg cctacctgct gagcaagaac aatgccattg agcccaggag cttccagaag 2820
aagaccaggc actacttcat tgctgctgtg gagaggctgt gggactatgg catgagcagc 2880
agcccccatg tgctgaggaa cagggcccag tctggctctg tgccccagtt caagaaggtg 2940
gtgttccagg agttcactga tggcagcttc acccagcccc tgtacagagg ggagctgaat 3000
gagcacctgg gcctgctggg cccctacatc agggctgagg tggaggacaa catcatggtg 3060
accttcagga accaggccag caggccctac agcttctaca gcagcctgat cagctatgag 3120
gaggaccaga ggcagggggc tgagcccagg aagaactttg tgaagcccaa tgaaaccaag 3180
acctacttct ggaaggtgca gcaccacatg gcccccacca aggatgagtt tgactgcaag 3240
gcctgggcct acttctctga tgtggacctg gagaaggatg tgcactctgg cctgattggc 3300
cccctgctgg tgtgccacac caacaccctg aaccctgccc atggcaggca ggtgactgtg 3360
caggagtttg ccctgttctt caccatcttt gatgaaacca agagctggta cttcactgag 3420
aacatggaga ggaactgcag ggccccctgc aacatccaga tggaggaccc caccttcaag 3480
gagaactaca ggttccatgc catcaatggc tacatcatgg acaccctgcc tggcctggtg 3540
atggcccagg accagaggat caggtggtac ctgctgagca tgggcagcaa tgagaacatc 3600
cacagcatcc acttctctgg ccatgtgttc actgtgagga agaaggagga gtacaagatg 3660
gccctgtaca acctgtaccc tggggtgttt gagactgtgg agatgctgcc cagcaaggct 3720
ggcatctgga gggtggagtg cctgattggg gagcacctgc atgctggcat gagcaccctg 3780
ttcctggtgt acagcaacaa gtgccagacc cccctgggca tggcctctgg ccacatcagg 3840
gacttccaga tcactgcctc tggccagtat ggccagtggg cccccaagct ggccaggctg 3900
cactactctg gcagcatcaa tgcctggagc accaaggagc ccttcagctg gatcaaggtg 3960
gacctgctgg cccccatgat catccatggc atcaagaccc agggggccag gcagaagttc 4020
agcagcctgt acatcagcca gttcatcatc atgtacagcc tggatggcaa gaagtggcag 4080
acctacaggg gcaacagcac tggcaccctg atggtgttct ttggcaatgt ggacagctct 4140
ggcatcaagc acaacatctt caaccccccc atcattgcca gatacatcag gctgcacccc 4200
acccactaca gcatcaggag caccctgagg atggagctga tgggctgtga cctgaacagc 4260
tgcagcatgc ccctgggcat ggagagcaag gccatctctg atgcccagat cactgccagc 4320
agctacttca ccaacatgtt tgccacctgg agccccagca aggccaggct gcacctgcag 4380
ggcaggagca atgcctggag gccccaggtc aacaacccca aggagtggct gcaggtggac 4440
ttccagaaga ccatgaaggt gactggggtg accacccagg gggtgaagag cctgctgacc 4500
agcatgtatg tgaaggagtt cctgatcagc agcagccagg atggccacca gtggaccctg 4560
ttcttccaga atggcaaggt gaaggtgttc cagggcaacc aggacagctt cacccctgtg 4620
gtgaacagcc tggacccccc cctgctgacc agatacctga ggattcaccc ccagagctgg 4680
gtgcaccaga ttgccctgag gatggaggtg ctgggctgtg aggcccagga cctgtactga 4740
aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtga ggaaccccta 4800
gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca 4860
aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga 4920
gagggagtgg ccaa 4934
<210> 9
<211> 5511
<212> DNA
<213> 腺相关病毒2
<400> 9
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgtaa gtactagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg 780
gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc 840
ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg 900
gcaaagaatt gcgatcgcca ccatgcagat tgagctgagc acctgcttct tcctgtgcct 960
gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg agctgagctg 1020
ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc cccccagagt 1080
gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt ttgtggagtt 1140
cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc tgctgggccc 1200
caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca tggccagcca 1260
ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg gggctgagta 1320
tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg ggggcagcca 1380
cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc ccctgtgcct 1440
gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg gcctgattgg 1500
ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga ccctgcacaa 1560
gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg aaaccaagaa 1620
cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga tgcacactgt 1680
gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga agtctgtgta 1740
ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc tggagggcca 1800
caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca tcaccttcct 1860
gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc acatcagcag 1920
ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg aggagcccca 1980
gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga ctgactctga 2040
gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga tcaggtctgt 2100
ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg aggactggga 2160
ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt acctgaacaa 2220
tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct acactgatga 2280
aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc ccctgctgta 2340
tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca ggccctacaa 2400
catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc tgcccaaggg 2460
ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt acaagtggac 2520
tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca gatactacag 2580
cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc tgctgatctg 2640
ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga ggaatgtgat 2700
cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca tccagaggtt 2760
cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca gcaacatcat 2820
gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc tgcatgaggt 2880
ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg tgttcttctc 2940
tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt tccccttctc 3000
tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg gctgccacaa 3060
ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct gtgacaagaa 3120
cactggggac tactatgagg acagctatga ggacatctct gcctacctgc tgagcaagaa 3180
caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga ggcaccagag 3240
ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg atgacaccat 3300
ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga accagagccc 3360
caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga ggctgtggga 3420
ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg gctctgtgcc 3480
ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc agcccctgta 3540
cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg ctgaggtgga 3600
ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct tctacagcag 3660
cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga actttgtgaa 3720
gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc ccaccaagga 3780
tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga aggatgtgca 3840
ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc ctgcccatgg 3900
caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg aaaccaagag 3960
ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca tccagatgga 4020
ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca tcatggacac 4080
cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc tgagcatggg 4140
cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg tgaggaagaa 4200
ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga ctgtggagat 4260
gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc acctgcatgc 4320
tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc tgggcatggc 4380
ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc agtgggcccc 4440
caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca aggagccctt 4500
cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca agacccaggg 4560
ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt acagcctgga 4620
tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg tgttctttgg 4680
caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca ttgccagata 4740
catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg agctgatggg 4800
ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca tctctgatgc 4860
ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc ccagcaaggc 4920
caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca accccaagga 4980
gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca cccagggggt 5040
gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca gccaggatgg 5100
ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg gcaaccagga 5160
cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat acctgaggat 5220
tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg gctgtgaggc 5280
ccaggacctg tactgacctc gaggaataaa ggaaatttat tttcattgca atagtgtgtt 5340
ggttttttgt gtcacgtggc ggccgcagga acccctagtg atggagttgg ccactccctc 5400
tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 5460
tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca a 5511
<210> 10
<211> 5688
<212> DNA
<213> 腺相关病毒2
<400> 10
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgtaa gtactagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg 780
gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc 840
ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg 900
gcaaagaatt gcgatcgcca ccatgcagat tgagctgagc acctgcttct tcctgtgcct 960
gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg agctgagctg 1020
ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc cccccagagt 1080
gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt ttgtggagtt 1140
cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc tgctgggccc 1200
caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca tggccagcca 1260
ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg gggctgagta 1320
tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg ggggcagcca 1380
cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc ccctgtgcct 1440
gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg gcctgattgg 1500
ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga ccctgcacaa 1560
gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg aaaccaagaa 1620
cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga tgcacactgt 1680
gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga agtctgtgta 1740
ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc tggagggcca 1800
caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca tcaccttcct 1860
gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc acatcagcag 1920
ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg aggagcccca 1980
gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga ctgactctga 2040
gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga tcaggtctgt 2100
ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg aggactggga 2160
ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt acctgaacaa 2220
tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct acactgatga 2280
aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc ccctgctgta 2340
tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca ggccctacaa 2400
catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc tgcccaaggg 2460
ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt acaagtggac 2520
tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca gatactacag 2580
cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc tgctgatctg 2640
ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga ggaatgtgat 2700
cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca tccagaggtt 2760
cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca gcaacatcat 2820
gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc tgcatgaggt 2880
ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg tgttcttctc 2940
tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt tccccttctc 3000
tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg gctgccacaa 3060
ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct gtgacaagaa 3120
cactggggac tactatgagg acagctatga ggacatctct gcctacctgc tgagcaagaa 3180
caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga ggcaccagag 3240
ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg atgacaccat 3300
ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga accagagccc 3360
caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga ggctgtggga 3420
ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg gctctgtgcc 3480
ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc agcccctgta 3540
cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg ctgaggtgga 3600
ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct tctacagcag 3660
cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga actttgtgaa 3720
gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc ccaccaagga 3780
tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga aggatgtgca 3840
ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc ctgcccatgg 3900
caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg aaaccaagag 3960
ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca tccagatgga 4020
ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca tcatggacac 4080
cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc tgagcatggg 4140
cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg tgaggaagaa 4200
ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga ctgtggagat 4260
gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc acctgcatgc 4320
tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc tgggcatggc 4380
ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc agtgggcccc 4440
caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca aggagccctt 4500
cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca agacccaggg 4560
ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt acagcctgga 4620
tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg tgttctttgg 4680
caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca ttgccagata 4740
catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg agctgatggg 4800
ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca tctctgatgc 4860
ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc ccagcaaggc 4920
caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca accccaagga 4980
gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca cccagggggt 5040
gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca gccaggatgg 5100
ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg gcaaccagga 5160
cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat acctgaggat 5220
tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg gctgtgaggc 5280
ccaggacctg tactgacctc gaggtgtgcc ttctagttgc cagccatctg ttgtttgccc 5340
ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 5400
tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 5460
gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 5520
ctctatgggc acgtggcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct 5580
gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc 5640
ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaa 5688
<210> 11
<211> 5613
<212> DNA
<213> 腺相关病毒2
<400> 11
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgtaa gtactagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg 780
gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc 840
ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg 900
gcaaagaatt gcgatcgcca ccatgcagat tgagctgagc acctgcttct tcctgtgcct 960
gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg agctgagctg 1020
ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc cccccagagt 1080
gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt ttgtggagtt 1140
cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc tgctgggccc 1200
caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca tggccagcca 1260
ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg gggctgagta 1320
tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg ggggcagcca 1380
cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc ccctgtgcct 1440
gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg gcctgattgg 1500
ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga ccctgcacaa 1560
gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg aaaccaagaa 1620
cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga tgcacactgt 1680
gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga agtctgtgta 1740
ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc tggagggcca 1800
caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca tcaccttcct 1860
gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc acatcagcag 1920
ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg aggagcccca 1980
gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga ctgactctga 2040
gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga tcaggtctgt 2100
ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg aggactggga 2160
ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt acctgaacaa 2220
tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct acactgatga 2280
aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc ccctgctgta 2340
tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca ggccctacaa 2400
catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc tgcccaaggg 2460
ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt acaagtggac 2520
tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca gatactacag 2580
cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc tgctgatctg 2640
ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga ggaatgtgat 2700
cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca tccagaggtt 2760
cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca gcaacatcat 2820
gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc tgcatgaggt 2880
ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg tgttcttctc 2940
tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt tccccttctc 3000
tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg gctgccacaa 3060
ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct gtgacaagaa 3120
cactggggac tactatgagg acagctatga ggacatctct gcctacctgc tgagcaagaa 3180
caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga ggcaccagag 3240
ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg atgacaccat 3300
ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga accagagccc 3360
caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga ggctgtggga 3420
ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg gctctgtgcc 3480
ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc agcccctgta 3540
cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg ctgaggtgga 3600
ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct tctacagcag 3660
cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga actttgtgaa 3720
gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc ccaccaagga 3780
tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga aggatgtgca 3840
ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc ctgcccatgg 3900
caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg aaaccaagag 3960
ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca tccagatgga 4020
ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca tcatggacac 4080
cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc tgagcatggg 4140
cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg tgaggaagaa 4200
ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga ctgtggagat 4260
gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc acctgcatgc 4320
tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc tgggcatggc 4380
ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc agtgggcccc 4440
caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca aggagccctt 4500
cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca agacccaggg 4560
ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt acagcctgga 4620
tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg tgttctttgg 4680
caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca ttgccagata 4740
catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg agctgatggg 4800
ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca tctctgatgc 4860
ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc ccagcaaggc 4920
caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca accccaagga 4980
gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca cccagggggt 5040
gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca gccaggatgg 5100
ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg gcaaccagga 5160
cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat acctgaggat 5220
tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg gctgtgaggc 5280
ccaggacctg tactgacctc gaggcactgt cctttcctaa taaaatgagg aaattgcatc 5340
gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 5400
ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggcacgtg 5460
gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct 5520
cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt 5580
gagcgagcga gcgcgcagag agggagtggc caa 5613
<210> 12
<211> 5362
<212> DNA
<213> 腺相关病毒2
<400> 12
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgact 360
ggacacagga cgctgtggtt tctgagccag ggggcgactc agatcccagc cagtggactt 420
agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc 480
ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc 540
agcttcaggc accaccactg acctgggaca gtgaatcgta agtactagca gctacaatcc 600
agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc 660
taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac 720
gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat tgcgatcgcc accatgcaga 780
ttgagctgag cacctgcttc ttcctgtgcc tgctgaggtt ctgcttctct gccaccagga 840
gatactacct gggggctgtg gagctgagct gggactacat gcagtctgac ctgggggagc 900
tgcctgtgga tgccaggttc ccccccagag tgcccaagag cttccccttc aacacctctg 960
tggtgtacaa gaagaccctg tttgtggagt tcactgacca cctgttcaac attgccaagc 1020
ccaggccccc ctggatgggc ctgctgggcc ccaccatcca ggctgaggtg tatgacactg 1080
tggtgatcac cctgaagaac atggccagcc accctgtgag cctgcatgct gtgggggtga 1140
gctactggaa ggcctctgag ggggctgagt atgatgacca gaccagccag agggagaagg 1200
aggatgacaa ggtgttccct gggggcagcc acacctatgt gtggcaggtg ctgaaggaga 1260
atggccccat ggcctctgac cccctgtgcc tgacctacag ctacctgagc catgtggacc 1320
tggtgaagga cctgaactct ggcctgattg gggccctgct ggtgtgcagg gagggcagcc 1380
tggccaagga gaagacccag accctgcaca agttcatcct gctgtttgct gtgtttgatg 1440
agggcaagag ctggcactct gaaaccaaga acagcctgat gcaggacagg gatgctgcct 1500
ctgccagggc ctggcccaag atgcacactg tgaatggcta tgtgaacagg agcctgcctg 1560
gcctgattgg ctgccacagg aagtctgtgt actggcatgt gattggcatg ggcaccaccc 1620
ctgaggtgca cagcatcttc ctggagggcc acaccttcct ggtcaggaac cacaggcagg 1680
ccagcctgga gatcagcccc atcaccttcc tgactgccca gaccctgctg atggacctgg 1740
gccagttcct gctgttctgc cacatcagca gccaccagca tgatggcatg gaggcctatg 1800
tgaaggtgga cagctgccct gaggagcccc agctgaggat gaagaacaat gaggaggctg 1860
aggactatga tgatgacctg actgactctg agatggatgt ggtgaggttt gatgatgaca 1920
acagccccag cttcatccag atcaggtctg tggccaagaa gcaccccaag acctgggtgc 1980
actacattgc tgctgaggag gaggactggg actatgcccc cctggtgctg gcccctgatg 2040
acaggagcta caagagccag tacctgaaca atggccccca gaggattggc aggaagtaca 2100
agaaggtcag gttcatggcc tacactgatg aaaccttcaa gaccagggag gccatccagc 2160
atgagtctgg catcctgggc cccctgctgt atggggaggt gggggacacc ctgctgatca 2220
tcttcaagaa ccaggccagc aggccctaca acatctaccc ccatggcatc actgatgtga 2280
ggcccctgta cagcaggagg ctgcccaagg gggtgaagca cctgaaggac ttccccatcc 2340
tgcctgggga gatcttcaag tacaagtgga ctgtgactgt ggaggatggc cccaccaagt 2400
ctgaccccag gtgcctgacc agatactaca gcagctttgt gaacatggag agggacctgg 2460
cctctggcct gattggcccc ctgctgatct gctacaagga gtctgtggac cagaggggca 2520
accagatcat gtctgacaag aggaatgtga tcctgttctc tgtgtttgat gagaacagga 2580
gctggtacct gactgagaac atccagaggt tcctgcccaa ccctgctggg gtgcagctgg 2640
aggaccctga gttccaggcc agcaacatca tgcacagcat caatggctat gtgtttgaca 2700
gcctgcagct gtctgtgtgc ctgcatgagg tggcctactg gtacatcctg agcattgggg 2760
cccagactga cttcctgtct gtgttcttct ctggctacac cttcaagcac aagatggtgt 2820
atgaggacac cctgaccctg ttccccttct ctggggagac tgtgttcatg agcatggaga 2880
accctggcct gtggattctg ggctgccaca actctgactt caggaacagg ggcatgactg 2940
ccctgctgaa agtctccagc tgtgacaaga acactgggga ctactatgag gacagctatg 3000
aggacatctc tgcctacctg ctgagcaaga acaatgccat tgagcccagg agcttcagcc 3060
agaacccccc agtgctgaag aggcaccaga gggagatcac caggaccacc ctgcagtctg 3120
accaggagga gattgactat gatgacacca tctctgtgga gatgaagaag gaggactttg 3180
acatctacga cgaggacgag aaccagagcc ccaggagctt ccagaagaag accaggcact 3240
acttcattgc tgctgtggag aggctgtggg actatggcat gagcagcagc ccccatgtgc 3300
tgaggaacag ggcccagtct ggctctgtgc cccagttcaa gaaggtggtg ttccaggagt 3360
tcactgatgg cagcttcacc cagcccctgt acagagggga gctgaatgag cacctgggcc 3420
tgctgggccc ctacatcagg gctgaggtgg aggacaacat catggtgacc ttcaggaacc 3480
aggccagcag gccctacagc ttctacagca gcctgatcag ctatgaggag gaccagaggc 3540
agggggctga gcccaggaag aactttgtga agcccaatga aaccaagacc tacttctgga 3600
aggtgcagca ccacatggcc cccaccaagg atgagtttga ctgcaaggcc tgggcctact 3660
tctctgatgt ggacctggag aaggatgtgc actctggcct gattggcccc ctgctggtgt 3720
gccacaccaa caccctgaac cctgcccatg gcaggcaggt gactgtgcag gagtttgccc 3780
tgttcttcac catctttgat gaaaccaaga gctggtactt cactgagaac atggagagga 3840
actgcagggc cccctgcaac atccagatgg aggaccccac cttcaaggag aactacaggt 3900
tccatgccat caatggctac atcatggaca ccctgcctgg cctggtgatg gcccaggacc 3960
agaggatcag gtggtacctg ctgagcatgg gcagcaatga gaacatccac agcatccact 4020
tctctggcca tgtgttcact gtgaggaaga aggaggagta caagatggcc ctgtacaacc 4080
tgtaccctgg ggtgtttgag actgtggaga tgctgcccag caaggctggc atctggaggg 4140
tggagtgcct gattggggag cacctgcatg ctggcatgag caccctgttc ctggtgtaca 4200
gcaacaagtg ccagaccccc ctgggcatgg cctctggcca catcagggac ttccagatca 4260
ctgcctctgg ccagtatggc cagtgggccc ccaagctggc caggctgcac tactctggca 4320
gcatcaatgc ctggagcacc aaggagccct tcagctggat caaggtggac ctgctggccc 4380
ccatgatcat ccatggcatc aagacccagg gggccaggca gaagttcagc agcctgtaca 4440
tcagccagtt catcatcatg tacagcctgg atggcaagaa gtggcagacc tacaggggca 4500
acagcactgg caccctgatg gtgttctttg gcaatgtgga cagctctggc atcaagcaca 4560
acatcttcaa cccccccatc attgccagat acatcaggct gcaccccacc cactacagca 4620
tcaggagcac cctgaggatg gagctgatgg gctgtgacct gaacagctgc agcatgcccc 4680
tgggcatgga gagcaaggcc atctctgatg cccagatcac tgccagcagc tacttcacca 4740
acatgtttgc cacctggagc cccagcaagg ccaggctgca cctgcagggc aggagcaatg 4800
cctggaggcc ccaggtcaac aaccccaagg agtggctgca ggtggacttc cagaagacca 4860
tgaaggtgac tggggtgacc acccaggggg tgaagagcct gctgaccagc atgtatgtga 4920
aggagttcct gatcagcagc agccaggatg gccaccagtg gaccctgttc ttccagaatg 4980
gcaaggtgaa ggtgttccag ggcaaccagg acagcttcac ccctgtggtg aacagcctgg 5040
acccccccct gctgaccaga tacctgagga ttcaccccca gagctgggtg caccagattg 5100
ccctgaggat ggaggtgctg ggctgtgagg cccaggacct gtactgacct cgaggaataa 5160
aggaaattta ttttcattgc aatagtgtgt tggttttttg tgtcacgtgg cggccgcagg 5220
aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 5280
ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 5340
cgcgcagaga gggagtggcc aa 5362
<210> 13
<211> 5464
<212> DNA
<213> 腺相关病毒2
<400> 13
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgact 360
ggacacagga cgctgtggtt tctgagccag ggggcgactc agatcccagc cagtggactt 420
agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc 480
ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc 540
agcttcaggc accaccactg acctgggaca gtgaatcgta agtactagca gctacaatcc 600
agctaccatt ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc 660
taggcccttt tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac 720
gtgctggtct gtgtgctggc ccatcacttt ggcaaagaat tgcgatcgcc accatgcaga 780
ttgagctgag cacctgcttc ttcctgtgcc tgctgaggtt ctgcttctct gccaccagga 840
gatactacct gggggctgtg gagctgagct gggactacat gcagtctgac ctgggggagc 900
tgcctgtgga tgccaggttc ccccccagag tgcccaagag cttccccttc aacacctctg 960
tggtgtacaa gaagaccctg tttgtggagt tcactgacca cctgttcaac attgccaagc 1020
ccaggccccc ctggatgggc ctgctgggcc ccaccatcca ggctgaggtg tatgacactg 1080
tggtgatcac cctgaagaac atggccagcc accctgtgag cctgcatgct gtgggggtga 1140
gctactggaa ggcctctgag ggggctgagt atgatgacca gaccagccag agggagaagg 1200
aggatgacaa ggtgttccct gggggcagcc acacctatgt gtggcaggtg ctgaaggaga 1260
atggccccat ggcctctgac cccctgtgcc tgacctacag ctacctgagc catgtggacc 1320
tggtgaagga cctgaactct ggcctgattg gggccctgct ggtgtgcagg gagggcagcc 1380
tggccaagga gaagacccag accctgcaca agttcatcct gctgtttgct gtgtttgatg 1440
agggcaagag ctggcactct gaaaccaaga acagcctgat gcaggacagg gatgctgcct 1500
ctgccagggc ctggcccaag atgcacactg tgaatggcta tgtgaacagg agcctgcctg 1560
gcctgattgg ctgccacagg aagtctgtgt actggcatgt gattggcatg ggcaccaccc 1620
ctgaggtgca cagcatcttc ctggagggcc acaccttcct ggtcaggaac cacaggcagg 1680
ccagcctgga gatcagcccc atcaccttcc tgactgccca gaccctgctg atggacctgg 1740
gccagttcct gctgttctgc cacatcagca gccaccagca tgatggcatg gaggcctatg 1800
tgaaggtgga cagctgccct gaggagcccc agctgaggat gaagaacaat gaggaggctg 1860
aggactatga tgatgacctg actgactctg agatggatgt ggtgaggttt gatgatgaca 1920
acagccccag cttcatccag atcaggtctg tggccaagaa gcaccccaag acctgggtgc 1980
actacattgc tgctgaggag gaggactggg actatgcccc cctggtgctg gcccctgatg 2040
acaggagcta caagagccag tacctgaaca atggccccca gaggattggc aggaagtaca 2100
agaaggtcag gttcatggcc tacactgatg aaaccttcaa gaccagggag gccatccagc 2160
atgagtctgg catcctgggc cccctgctgt atggggaggt gggggacacc ctgctgatca 2220
tcttcaagaa ccaggccagc aggccctaca acatctaccc ccatggcatc actgatgtga 2280
ggcccctgta cagcaggagg ctgcccaagg gggtgaagca cctgaaggac ttccccatcc 2340
tgcctgggga gatcttcaag tacaagtgga ctgtgactgt ggaggatggc cccaccaagt 2400
ctgaccccag gtgcctgacc agatactaca gcagctttgt gaacatggag agggacctgg 2460
cctctggcct gattggcccc ctgctgatct gctacaagga gtctgtggac cagaggggca 2520
accagatcat gtctgacaag aggaatgtga tcctgttctc tgtgtttgat gagaacagga 2580
gctggtacct gactgagaac atccagaggt tcctgcccaa ccctgctggg gtgcagctgg 2640
aggaccctga gttccaggcc agcaacatca tgcacagcat caatggctat gtgtttgaca 2700
gcctgcagct gtctgtgtgc ctgcatgagg tggcctactg gtacatcctg agcattgggg 2760
cccagactga cttcctgtct gtgttcttct ctggctacac cttcaagcac aagatggtgt 2820
atgaggacac cctgaccctg ttccccttct ctggggagac tgtgttcatg agcatggaga 2880
accctggcct gtggattctg ggctgccaca actctgactt caggaacagg ggcatgactg 2940
ccctgctgaa agtctccagc tgtgacaaga acactgggga ctactatgag gacagctatg 3000
aggacatctc tgcctacctg ctgagcaaga acaatgccat tgagcccagg agcttcagcc 3060
agaacccccc agtgctgaag aggcaccaga gggagatcac caggaccacc ctgcagtctg 3120
accaggagga gattgactat gatgacacca tctctgtgga gatgaagaag gaggactttg 3180
acatctacga cgaggacgag aaccagagcc ccaggagctt ccagaagaag accaggcact 3240
acttcattgc tgctgtggag aggctgtggg actatggcat gagcagcagc ccccatgtgc 3300
tgaggaacag ggcccagtct ggctctgtgc cccagttcaa gaaggtggtg ttccaggagt 3360
tcactgatgg cagcttcacc cagcccctgt acagagggga gctgaatgag cacctgggcc 3420
tgctgggccc ctacatcagg gctgaggtgg aggacaacat catggtgacc ttcaggaacc 3480
aggccagcag gccctacagc ttctacagca gcctgatcag ctatgaggag gaccagaggc 3540
agggggctga gcccaggaag aactttgtga agcccaatga aaccaagacc tacttctgga 3600
aggtgcagca ccacatggcc cccaccaagg atgagtttga ctgcaaggcc tgggcctact 3660
tctctgatgt ggacctggag aaggatgtgc actctggcct gattggcccc ctgctggtgt 3720
gccacaccaa caccctgaac cctgcccatg gcaggcaggt gactgtgcag gagtttgccc 3780
tgttcttcac catctttgat gaaaccaaga gctggtactt cactgagaac atggagagga 3840
actgcagggc cccctgcaac atccagatgg aggaccccac cttcaaggag aactacaggt 3900
tccatgccat caatggctac atcatggaca ccctgcctgg cctggtgatg gcccaggacc 3960
agaggatcag gtggtacctg ctgagcatgg gcagcaatga gaacatccac agcatccact 4020
tctctggcca tgtgttcact gtgaggaaga aggaggagta caagatggcc ctgtacaacc 4080
tgtaccctgg ggtgtttgag actgtggaga tgctgcccag caaggctggc atctggaggg 4140
tggagtgcct gattggggag cacctgcatg ctggcatgag caccctgttc ctggtgtaca 4200
gcaacaagtg ccagaccccc ctgggcatgg cctctggcca catcagggac ttccagatca 4260
ctgcctctgg ccagtatggc cagtgggccc ccaagctggc caggctgcac tactctggca 4320
gcatcaatgc ctggagcacc aaggagccct tcagctggat caaggtggac ctgctggccc 4380
ccatgatcat ccatggcatc aagacccagg gggccaggca gaagttcagc agcctgtaca 4440
tcagccagtt catcatcatg tacagcctgg atggcaagaa gtggcagacc tacaggggca 4500
acagcactgg caccctgatg gtgttctttg gcaatgtgga cagctctggc atcaagcaca 4560
acatcttcaa cccccccatc attgccagat acatcaggct gcaccccacc cactacagca 4620
tcaggagcac cctgaggatg gagctgatgg gctgtgacct gaacagctgc agcatgcccc 4680
tgggcatgga gagcaaggcc atctctgatg cccagatcac tgccagcagc tacttcacca 4740
acatgtttgc cacctggagc cccagcaagg ccaggctgca cctgcagggc aggagcaatg 4800
cctggaggcc ccaggtcaac aaccccaagg agtggctgca ggtggacttc cagaagacca 4860
tgaaggtgac tggggtgacc acccaggggg tgaagagcct gctgaccagc atgtatgtga 4920
aggagttcct gatcagcagc agccaggatg gccaccagtg gaccctgttc ttccagaatg 4980
gcaaggtgaa ggtgttccag ggcaaccagg acagcttcac ccctgtggtg aacagcctgg 5040
acccccccct gctgaccaga tacctgagga ttcaccccca gagctgggtg caccagattg 5100
ccctgaggat ggaggtgctg ggctgtgagg cccaggacct gtactgacct cgaggcactg 5160
tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 5220
tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 5280
ctggggatgc ggtgggctct atgggcacgt ggcggccgca ggaaccccta gtgatggagt 5340
tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 5400
gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg 5460
ccaa 5464
<210> 14
<211> 6354
<212> DNA
<213> 腺相关病毒2
<400> 14
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tttaaacgtc gacaggttaa 180
tttttaaaaa gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc 240
tctggttaat aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc 300
tggacttatc ctctgggcct ctccccaccc ccaggagagg ctcaggttaa tttttaaaaa 360
gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc tctggttaat 420
aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc tggacttatc 480
ctctgggcct ctccccaccc ccaggagagg ctgtcgactg gacacaggac gctgtggttt 540
ctgagccagg gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga 600
taactggggt gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc 660
actgcttaaa tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga 720
cctgggacag tgaatcgtaa gtactagcag ctacaatcca gctaccattc tgcttttatt 780
ttatggttgg gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg 840
ttcatacctc ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc 900
catcactttg gcaaagaatt gcgatcgcca ccatgcagat tgagctgagc acctgcttct 960
tcctgtgcct gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg 1020
agctgagctg ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc 1080
cccccagagt gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt 1140
ttgtggagtt cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc 1200
tgctgggccc caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca 1260
tggccagcca ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg 1320
gggctgagta tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg 1380
ggggcagcca cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc 1440
ccctgtgcct gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg 1500
gcctgattgg ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga 1560
ccctgcacaa gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg 1620
aaaccaagaa cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga 1680
tgcacactgt gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga 1740
agtctgtgta ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc 1800
tggagggcca caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca 1860
tcaccttcct gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc 1920
acatcagcag ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg 1980
aggagcccca gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga 2040
ctgactctga gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga 2100
tcaggtctgt ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg 2160
aggactggga ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt 2220
acctgaacaa tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct 2280
acactgatga aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc 2340
ccctgctgta tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca 2400
ggccctacaa catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc 2460
tgcccaaggg ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt 2520
acaagtggac tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca 2580
gatactacag cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc 2640
tgctgatctg ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga 2700
ggaatgtgat cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca 2760
tccagaggtt cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca 2820
gcaacatcat gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc 2880
tgcatgaggt ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg 2940
tgttcttctc tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt 3000
tccccttctc tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg 3060
gctgccacaa ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct 3120
gtgacaagaa cactggggac tactatgagg acagctatga ggacatctct gcctacctgc 3180
tgagcaagaa caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga 3240
ggcaccagag ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg 3300
atgacaccat ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga 3360
accagagccc caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga 3420
ggctgtggga ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg 3480
gctctgtgcc ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc 3540
agcccctgta cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg 3600
ctgaggtgga ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct 3660
tctacagcag cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga 3720
actttgtgaa gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc 3780
ccaccaagga tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga 3840
aggatgtgca ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc 3900
ctgcccatgg caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg 3960
aaaccaagag ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca 4020
tccagatgga ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca 4080
tcatggacac cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc 4140
tgagcatggg cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg 4200
tgaggaagaa ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga 4260
ctgtggagat gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc 4320
acctgcatgc tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc 4380
tgggcatggc ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc 4440
agtgggcccc caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca 4500
aggagccctt cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca 4560
agacccaggg ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt 4620
acagcctgga tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg 4680
tgttctttgg caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca 4740
ttgccagata catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg 4800
agctgatggg ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca 4860
tctctgatgc ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc 4920
ccagcaaggc caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca 4980
accccaagga gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca 5040
cccagggggt gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca 5100
gccaggatgg ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg 5160
gcaaccagga cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat 5220
acctgaggat tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg 5280
gctgtgaggc ccaggacctg tactgacctc gaggtgtgcc ttctagttgc cagccatctg 5340
ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 5400
cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 5460
gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 5520
atgcggtggg ctctatgggc acgtgccctc tcacactacc taaaccacgc caggacaacc 5580
tctgctcctc tccaccgaaa ttccaagggg tcgagtggat gttggaggtg gcatgggccc 5640
agagaggtct ctgacctctg ccccagctcc aaggtcagca ggcagggagg gctgtgtgtt 5700
tgctgtttgc tgcttgcaat gtttgcccat tttagggaca tgagtaggct gaagtttgtt 5760
cagtgtggac ttcagaggca gcacacaaac agctgctgga ggatgggaac tgaggggttg 5820
gaagggggca gggtgagccc agaaactcct gtgtgcctct gagcctgcag ccctctcaca 5880
ctacctaaac cacgccagga caacctctgc tcctctccac cgaaattcca aggggtcgag 5940
tggatgttgg aggtggcatg ggcccagaga ggtctctgac ctctgcccca gctccaaggt 6000
cagcaggcag ggagggctgt gtgtttgctg tttgctgctt gcaatgtttg cccattttag 6060
ggacatgagt aggctgaagt ttgttcagtg tggacttcag aggcagcaca caaacagctg 6120
ctggaggatg ggaactgagg ggttggaagg gggcagggtg agcccagaaa ctcctgtgtg 6180
cctctgagcc tgcagcacgt ggcggccgca ggaaccccta gtgatggagt tggccactcc 6240
ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 6300
ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaa 6354
<210> 15
<211> 6308
<212> DNA
<213> 腺相关病毒2
<400> 15
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttaattttta aaaagcagtc 180
aaaagtccaa gtggcccttg gcagcattta ctctctctgt ttgctctggt taataatctc 240
aggagcacaa acattcctgg aggcaggaga agaaatcaac atcctggact tatcctctgg 300
gcctgttaat ttttaaaaag cagtcaaaag tccaagtggc ccttggcagc atttactctc 360
tctgtttgct ctggttaata atctcaggag cacaaacatt cctggaggca ggagaagaaa 420
tcaacatcct ggacttatcc tctgggccta ggcctgaggc tggtcaaaat tgaacctcct 480
cctgctctga gcagcctggg gggcagacta agcagagggc tgtgcagacc cacataaaga 540
gcctactgtg tgccaggcac ttcacccgag gcacttcaca agcatgcttg ggaatgaaac 600
ttccaactct ttgggatgca ggtgaaacag ttcctggttc agagaggtga agcggcctgc 660
ctgaggcagc acagctcttc tttacagatg tgcttcccca cctctaccct gtctcacggc 720
cccccatgcc agcctgacgg ttgtgtctgc ctcagtcatg ctccattttt ccatcgggac 780
catcaagagg gtgtttgtgt ctaaggctga ctgggtaact ttggatgagc ggtctctccg 840
ctctgagcct gtttcctcat ctgtcaaatg ggctctaacc cactctgatc tcccagggcg 900
gcagtaagtc ttcagcatca ggcattttgg ggtgactcag taaatggtag atcttgctac 960
cagtggaaca gccactaagg attctgcagt gagagcagag ggccagctaa gtggtactct 1020
cccagagact gtctgactca cgccaccccc tccaccttgg acacaggacg ctgtggtttc 1080
tgagccaggt acaatgactc ctttcggtaa gtgcagtgga agctgtacac tgcccaggca 1140
aagcgtccgg gcagcgtagg cgggcgactc agatcccagc cagtggactt agcccctgtt 1200
tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc ccccgttgcc 1260
cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc agcttcaggc 1320
accaccactg acctgggaca gtgaatcgta agtactagca gctacaatcc agctaccatt 1380
ctgcttttat tttatggttg ggataaggct ggattattct gagtccaagc taggcccttt 1440
tgctaatcat gttcatacct cttatcttcc tcccacagct cctgggcaac gtgctggtct 1500
gtgtgctggc ccatcacttt ggcaaagaat tgcgatcgcc accatgcaga ttgagctgag 1560
cacctgcttc ttcctgtgcc tgctgaggtt ctgcttctct gccaccagga gatactacct 1620
gggggctgtg gagctgagct gggactacat gcagtctgac ctgggggagc tgcctgtgga 1680
tgccaggttc ccccccagag tgcccaagag cttccccttc aacacctctg tggtgtacaa 1740
gaagaccctg tttgtggagt tcactgacca cctgttcaac attgccaagc ccaggccccc 1800
ctggatgggc ctgctgggcc ccaccatcca ggctgaggtg tatgacactg tggtgatcac 1860
cctgaagaac atggccagcc accctgtgag cctgcatgct gtgggggtga gctactggaa 1920
ggcctctgag ggggctgagt atgatgacca gaccagccag agggagaagg aggatgacaa 1980
ggtgttccct gggggcagcc acacctatgt gtggcaggtg ctgaaggaga atggccccat 2040
ggcctctgac cccctgtgcc tgacctacag ctacctgagc catgtggacc tggtgaagga 2100
cctgaactct ggcctgattg gggccctgct ggtgtgcagg gagggcagcc tggccaagga 2160
gaagacccag accctgcaca agttcatcct gctgtttgct gtgtttgatg agggcaagag 2220
ctggcactct gaaaccaaga acagcctgat gcaggacagg gatgctgcct ctgccagggc 2280
ctggcccaag atgcacactg tgaatggcta tgtgaacagg agcctgcctg gcctgattgg 2340
ctgccacagg aagtctgtgt actggcatgt gattggcatg ggcaccaccc ctgaggtgca 2400
cagcatcttc ctggagggcc acaccttcct ggtcaggaac cacaggcagg ccagcctgga 2460
gatcagcccc atcaccttcc tgactgccca gaccctgctg atggacctgg gccagttcct 2520
gctgttctgc cacatcagca gccaccagca tgatggcatg gaggcctatg tgaaggtgga 2580
cagctgccct gaggagcccc agctgaggat gaagaacaat gaggaggctg aggactatga 2640
tgatgacctg actgactctg agatggatgt ggtgaggttt gatgatgaca acagccccag 2700
cttcatccag atcaggtctg tggccaagaa gcaccccaag acctgggtgc actacattgc 2760
tgctgaggag gaggactggg actatgcccc cctggtgctg gcccctgatg acaggagcta 2820
caagagccag tacctgaaca atggccccca gaggattggc aggaagtaca agaaggtcag 2880
gttcatggcc tacactgatg aaaccttcaa gaccagggag gccatccagc atgagtctgg 2940
catcctgggc cccctgctgt atggggaggt gggggacacc ctgctgatca tcttcaagaa 3000
ccaggccagc aggccctaca acatctaccc ccatggcatc actgatgtga ggcccctgta 3060
cagcaggagg ctgcccaagg gggtgaagca cctgaaggac ttccccatcc tgcctgggga 3120
gatcttcaag tacaagtgga ctgtgactgt ggaggatggc cccaccaagt ctgaccccag 3180
gtgcctgacc agatactaca gcagctttgt gaacatggag agggacctgg cctctggcct 3240
gattggcccc ctgctgatct gctacaagga gtctgtggac cagaggggca accagatcat 3300
gtctgacaag aggaatgtga tcctgttctc tgtgtttgat gagaacagga gctggtacct 3360
gactgagaac atccagaggt tcctgcccaa ccctgctggg gtgcagctgg aggaccctga 3420
gttccaggcc agcaacatca tgcacagcat caatggctat gtgtttgaca gcctgcagct 3480
gtctgtgtgc ctgcatgagg tggcctactg gtacatcctg agcattgggg cccagactga 3540
cttcctgtct gtgttcttct ctggctacac cttcaagcac aagatggtgt atgaggacac 3600
cctgaccctg ttccccttct ctggggagac tgtgttcatg agcatggaga accctggcct 3660
gtggattctg ggctgccaca actctgactt caggaacagg ggcatgactg ccctgctgaa 3720
agtctccagc tgtgacaaga acactgggga ctactatgag gacagctatg aggacatctc 3780
tgcctacctg ctgagcaaga acaatgccat tgagcccagg agcttcagcc agaacccccc 3840
agtgctgaag aggcaccaga gggagatcac caggaccacc ctgcagtctg accaggagga 3900
gattgactat gatgacacca tctctgtgga gatgaagaag gaggactttg acatctacga 3960
cgaggacgag aaccagagcc ccaggagctt ccagaagaag accaggcact acttcattgc 4020
tgctgtggag aggctgtggg actatggcat gagcagcagc ccccatgtgc tgaggaacag 4080
ggcccagtct ggctctgtgc cccagttcaa gaaggtggtg ttccaggagt tcactgatgg 4140
cagcttcacc cagcccctgt acagagggga gctgaatgag cacctgggcc tgctgggccc 4200
ctacatcagg gctgaggtgg aggacaacat catggtgacc ttcaggaacc aggccagcag 4260
gccctacagc ttctacagca gcctgatcag ctatgaggag gaccagaggc agggggctga 4320
gcccaggaag aactttgtga agcccaatga aaccaagacc tacttctgga aggtgcagca 4380
ccacatggcc cccaccaagg atgagtttga ctgcaaggcc tgggcctact tctctgatgt 4440
ggacctggag aaggatgtgc actctggcct gattggcccc ctgctggtgt gccacaccaa 4500
caccctgaac cctgcccatg gcaggcaggt gactgtgcag gagtttgccc tgttcttcac 4560
catctttgat gaaaccaaga gctggtactt cactgagaac atggagagga actgcagggc 4620
cccctgcaac atccagatgg aggaccccac cttcaaggag aactacaggt tccatgccat 4680
caatggctac atcatggaca ccctgcctgg cctggtgatg gcccaggacc agaggatcag 4740
gtggtacctg ctgagcatgg gcagcaatga gaacatccac agcatccact tctctggcca 4800
tgtgttcact gtgaggaaga aggaggagta caagatggcc ctgtacaacc tgtaccctgg 4860
ggtgtttgag actgtggaga tgctgcccag caaggctggc atctggaggg tggagtgcct 4920
gattggggag cacctgcatg ctggcatgag caccctgttc ctggtgtaca gcaacaagtg 4980
ccagaccccc ctgggcatgg cctctggcca catcagggac ttccagatca ctgcctctgg 5040
ccagtatggc cagtgggccc ccaagctggc caggctgcac tactctggca gcatcaatgc 5100
ctggagcacc aaggagccct tcagctggat caaggtggac ctgctggccc ccatgatcat 5160
ccatggcatc aagacccagg gggccaggca gaagttcagc agcctgtaca tcagccagtt 5220
catcatcatg tacagcctgg atggcaagaa gtggcagacc tacaggggca acagcactgg 5280
caccctgatg gtgttctttg gcaatgtgga cagctctggc atcaagcaca acatcttcaa 5340
cccccccatc attgccagat acatcaggct gcaccccacc cactacagca tcaggagcac 5400
cctgaggatg gagctgatgg gctgtgacct gaacagctgc agcatgcccc tgggcatgga 5460
gagcaaggcc atctctgatg cccagatcac tgccagcagc tacttcacca acatgtttgc 5520
cacctggagc cccagcaagg ccaggctgca cctgcagggc aggagcaatg cctggaggcc 5580
ccaggtcaac aaccccaagg agtggctgca ggtggacttc cagaagacca tgaaggtgac 5640
tggggtgacc acccaggggg tgaagagcct gctgaccagc atgtatgtga aggagttcct 5700
gatcagcagc agccaggatg gccaccagtg gaccctgttc ttccagaatg gcaaggtgaa 5760
ggtgttccag ggcaaccagg acagcttcac ccctgtggtg aacagcctgg acccccccct 5820
gctgaccaga tacctgagga ttcaccccca gagctgggtg caccagattg ccctgaggat 5880
ggaggtgctg ggctgtgagg cccaggacct gtactgacct cgagctgtgc cttctagttg 5940
ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc 6000
cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc 6060
tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag 6120
gcatgctggg gatgcggtgg gctctatgga ccggtgcggc cgcaggaacc cctagtgatg 6180
gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc 6240
gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga 6300
gtggccaa 6308
<210> 16
<211> 5635
<212> DNA
<213> 腺相关病毒2
<400> 16
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttaattttta aaaagcagtc 180
aaaagtccaa gtggcccttg gcagcattta ctctctctgt ttgctctggt taataatctc 240
aggagcacaa acattcctgg aggcaggaga agaaatcaac atcctggact tatcctctgg 300
gcctgttaat ttttaaaaag cagtcaaaag tccaagtggc ccttggcagc atttactctc 360
tctgtttgct ctggttaata atctcaggag cacaaacatt cctggaggca ggagaagaaa 420
tcaacatcct ggacttatcc tctgggccta gtcgactgga cacaggacgc tgtggtttct 480
gagccagggg gcgactcaga tcccagccag tggacttagc ccctgtttgc tcctccgata 540
actggggtga ccttggttaa tattcaccag cagcctcccc cgttgcccct ctggatccac 600
tgcttaaata cggacgagga cagggccctg tctcctcagc ttcaggcacc accactgacc 660
tgggacagtg aatcgtaagt actagcagct acaatccagc taccattctg cttttatttt 720
atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt 780
catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca 840
tcactttggc aaagaattgc gatcgccacc atgcagattg agctgagcac ctgcttcttc 900
ctgtgcctgc tgaggttctg cttctctgcc accaggagat actacctggg ggctgtggag 960
ctgagctggg actacatgca gtctgacctg ggggagctgc ctgtggatgc caggttcccc 1020
cccagagtgc ccaagagctt ccccttcaac acctctgtgg tgtacaagaa gaccctgttt 1080
gtggagttca ctgaccacct gttcaacatt gccaagccca ggcccccctg gatgggcctg 1140
ctgggcccca ccatccaggc tgaggtgtat gacactgtgg tgatcaccct gaagaacatg 1200
gccagccacc ctgtgagcct gcatgctgtg ggggtgagct actggaaggc ctctgagggg 1260
gctgagtatg atgaccagac cagccagagg gagaaggagg atgacaaggt gttccctggg 1320
ggcagccaca cctatgtgtg gcaggtgctg aaggagaatg gccccatggc ctctgacccc 1380
ctgtgcctga cctacagcta cctgagccat gtggacctgg tgaaggacct gaactctggc 1440
ctgattgggg ccctgctggt gtgcagggag ggcagcctgg ccaaggagaa gacccagacc 1500
ctgcacaagt tcatcctgct gtttgctgtg tttgatgagg gcaagagctg gcactctgaa 1560
accaagaaca gcctgatgca ggacagggat gctgcctctg ccagggcctg gcccaagatg 1620
cacactgtga atggctatgt gaacaggagc ctgcctggcc tgattggctg ccacaggaag 1680
tctgtgtact ggcatgtgat tggcatgggc accacccctg aggtgcacag catcttcctg 1740
gagggccaca ccttcctggt caggaaccac aggcaggcca gcctggagat cagccccatc 1800
accttcctga ctgcccagac cctgctgatg gacctgggcc agttcctgct gttctgccac 1860
atcagcagcc accagcatga tggcatggag gcctatgtga aggtggacag ctgccctgag 1920
gagccccagc tgaggatgaa gaacaatgag gaggctgagg actatgatga tgacctgact 1980
gactctgaga tggatgtggt gaggtttgat gatgacaaca gccccagctt catccagatc 2040
aggtctgtgg ccaagaagca ccccaagacc tgggtgcact acattgctgc tgaggaggag 2100
gactgggact atgcccccct ggtgctggcc cctgatgaca ggagctacaa gagccagtac 2160
ctgaacaatg gcccccagag gattggcagg aagtacaaga aggtcaggtt catggcctac 2220
actgatgaaa ccttcaagac cagggaggcc atccagcatg agtctggcat cctgggcccc 2280
ctgctgtatg gggaggtggg ggacaccctg ctgatcatct tcaagaacca ggccagcagg 2340
ccctacaaca tctaccccca tggcatcact gatgtgaggc ccctgtacag caggaggctg 2400
cccaaggggg tgaagcacct gaaggacttc cccatcctgc ctggggagat cttcaagtac 2460
aagtggactg tgactgtgga ggatggcccc accaagtctg accccaggtg cctgaccaga 2520
tactacagca gctttgtgaa catggagagg gacctggcct ctggcctgat tggccccctg 2580
ctgatctgct acaaggagtc tgtggaccag aggggcaacc agatcatgtc tgacaagagg 2640
aatgtgatcc tgttctctgt gtttgatgag aacaggagct ggtacctgac tgagaacatc 2700
cagaggttcc tgcccaaccc tgctggggtg cagctggagg accctgagtt ccaggccagc 2760
aacatcatgc acagcatcaa tggctatgtg tttgacagcc tgcagctgtc tgtgtgcctg 2820
catgaggtgg cctactggta catcctgagc attggggccc agactgactt cctgtctgtg 2880
ttcttctctg gctacacctt caagcacaag atggtgtatg aggacaccct gaccctgttc 2940
cccttctctg gggagactgt gttcatgagc atggagaacc ctggcctgtg gattctgggc 3000
tgccacaact ctgacttcag gaacaggggc atgactgccc tgctgaaagt ctccagctgt 3060
gacaagaaca ctggggacta ctatgaggac agctatgagg acatctctgc ctacctgctg 3120
agcaagaaca atgccattga gcccaggagc ttcagccaga accccccagt gctgaagagg 3180
caccagaggg agatcaccag gaccaccctg cagtctgacc aggaggagat tgactatgat 3240
gacaccatct ctgtggagat gaagaaggag gactttgaca tctacgacga ggacgagaac 3300
cagagcccca ggagcttcca gaagaagacc aggcactact tcattgctgc tgtggagagg 3360
ctgtgggact atggcatgag cagcagcccc catgtgctga ggaacagggc ccagtctggc 3420
tctgtgcccc agttcaagaa ggtggtgttc caggagttca ctgatggcag cttcacccag 3480
cccctgtaca gaggggagct gaatgagcac ctgggcctgc tgggccccta catcagggct 3540
gaggtggagg acaacatcat ggtgaccttc aggaaccagg ccagcaggcc ctacagcttc 3600
tacagcagcc tgatcagcta tgaggaggac cagaggcagg gggctgagcc caggaagaac 3660
tttgtgaagc ccaatgaaac caagacctac ttctggaagg tgcagcacca catggccccc 3720
accaaggatg agtttgactg caaggcctgg gcctacttct ctgatgtgga cctggagaag 3780
gatgtgcact ctggcctgat tggccccctg ctggtgtgcc acaccaacac cctgaaccct 3840
gcccatggca ggcaggtgac tgtgcaggag tttgccctgt tcttcaccat ctttgatgaa 3900
accaagagct ggtacttcac tgagaacatg gagaggaact gcagggcccc ctgcaacatc 3960
cagatggagg accccacctt caaggagaac tacaggttcc atgccatcaa tggctacatc 4020
atggacaccc tgcctggcct ggtgatggcc caggaccaga ggatcaggtg gtacctgctg 4080
agcatgggca gcaatgagaa catccacagc atccacttct ctggccatgt gttcactgtg 4140
aggaagaagg aggagtacaa gatggccctg tacaacctgt accctggggt gtttgagact 4200
gtggagatgc tgcccagcaa ggctggcatc tggagggtgg agtgcctgat tggggagcac 4260
ctgcatgctg gcatgagcac cctgttcctg gtgtacagca acaagtgcca gacccccctg 4320
ggcatggcct ctggccacat cagggacttc cagatcactg cctctggcca gtatggccag 4380
tgggccccca agctggccag gctgcactac tctggcagca tcaatgcctg gagcaccaag 4440
gagcccttca gctggatcaa ggtggacctg ctggccccca tgatcatcca tggcatcaag 4500
acccaggggg ccaggcagaa gttcagcagc ctgtacatca gccagttcat catcatgtac 4560
agcctggatg gcaagaagtg gcagacctac aggggcaaca gcactggcac cctgatggtg 4620
ttctttggca atgtggacag ctctggcatc aagcacaaca tcttcaaccc ccccatcatt 4680
gccagataca tcaggctgca ccccacccac tacagcatca ggagcaccct gaggatggag 4740
ctgatgggct gtgacctgaa cagctgcagc atgcccctgg gcatggagag caaggccatc 4800
tctgatgccc agatcactgc cagcagctac ttcaccaaca tgtttgccac ctggagcccc 4860
agcaaggcca ggctgcacct gcagggcagg agcaatgcct ggaggcccca ggtcaacaac 4920
cccaaggagt ggctgcaggt ggacttccag aagaccatga aggtgactgg ggtgaccacc 4980
cagggggtga agagcctgct gaccagcatg tatgtgaagg agttcctgat cagcagcagc 5040
caggatggcc accagtggac cctgttcttc cagaatggca aggtgaaggt gttccagggc 5100
aaccaggaca gcttcacccc tgtggtgaac agcctggacc cccccctgct gaccagatac 5160
ctgaggattc acccccagag ctgggtgcac cagattgccc tgaggatgga ggtgctgggc 5220
tgtgaggccc aggacctgta ctgacctcga gctgtgcctt ctagttgcca gccatctgtt 5280
gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc 5340
taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt 5400
ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat 5460
gcggtgggct ctatggaccg gtgcggccgc aggaacccct agtgatggag ttggccactc 5520
cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 5580
gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg gccaa 5635
<210> 17
<211> 6962
<212> DNA
<213> 腺相关病毒2
<400> 17
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgta ggctcagagg cacacaggag 180
tttctgggct caccctgccc ccttccaacc cctcagttcc catcctccag cagctgtttg 240
tgtgctgcct ctgaagtcca cactgaacaa acttcagcct actcatgtcc ctaaaatggg 300
caaacattgc aagcagcaaa cagcaaacac acagccctcc ctgcctgctg accttggagc 360
tggggcagag gtcagagacc tctctgggcc catgccacct ccaacatcca ctcgacccct 420
tggaatttcg gtggagagga gcagaggttg tcctggcgtg gtttaggtag tgtgagaggg 480
gtcgacaggc tcagaggcac acaggagttt ctgggctcac cctgccccct tccaacccct 540
cagttcccat cctccagcag ctgtttgtgt gctgcctctg aagtccacac tgaacaaact 600
tcagcctact catgtcccta aaatgggcaa acattgcaag cagcaaacag caaacacaca 660
gccctccctg cctgctgacc ttggagctgg ggcagaggtc agagacctct ctgggcccat 720
gccacctcca acatccactc gaccccttgg aatttcggtg gagaggagca gaggttgtcc 780
tggcgtggtt taggtagtgt gagaggggtc gacgttaatt tttaaaaagc agtcaaaagt 840
ccaagtggcc cttggcagca tttactctct ctgtttgctc tggttaataa tctcaggagc 900
acaaacattc ctggaggcag gagaagaaat caacatcctg gacttatcct ctgggcctgt 960
taatttttaa aaagcagtca aaagtccaag tggcccttgg cagcatttac tctctctgtt 1020
tgctctggtt aataatctca ggagcacaaa cattcctgga ggcaggagaa gaaatcaaca 1080
tcctggactt atcctctggg cctaggcctg aggctggtca aaattgaacc tcctcctgct 1140
ctgagcagcc tggggggcag actaagcaga gggctgtgca gacccacata aagagcctac 1200
tgtgtgccag gcacttcacc cgaggcactt cacaagcatg cttgggaatg aaacttccaa 1260
ctctttggga tgcaggtgaa acagttcctg gttcagagag gtgaagcggc ctgcctgagg 1320
cagcacagct cttctttaca gatgtgcttc cccacctcta ccctgtctca cggcccccca 1380
tgccagcctg acggttgtgt ctgcctcagt catgctccat ttttccatcg ggaccatcaa 1440
gagggtgttt gtgtctaagg ctgactgggt aactttggat gagcggtctc tccgctctga 1500
gcctgtttcc tcatctgtca aatgggctct aacccactct gatctcccag ggcggcagta 1560
agtcttcagc atcaggcatt ttggggtgac tcagtaaatg gtagatcttg ctaccagtgg 1620
aacagccact aaggattctg cagtgagagc agagggccag ctaagtggta ctctcccaga 1680
gactgtctga ctcacgccac cccctccacc ttggacacag gacgctgtgg tttctgagcc 1740
aggtacaatg actcctttcg gtaagtgcag tggaagctgt acactgccca ggcaaagcgt 1800
ccgggcagcg taggcgggcg actcagatcc cagccagtgg acttagcccc tgtttgctcc 1860
tccgataact ggggtgacct tggttaatat tcaccagcag cctcccccgt tgcccctctg 1920
gatccactgc ttaaatacgg acgaggacag ggccctgtct cctcagcttc aggcaccacc 1980
actgacctgg gacagtgaat cgtaagtact agcagctaca atccagctac cattctgctt 2040
ttattttatg gttgggataa ggctggatta ttctgagtcc aagctaggcc cttttgctaa 2100
tcatgttcat acctcttatc ttcctcccac agctcctggg caacgtgctg gtctgtgtgc 2160
tggcccatca ctttggcaaa gaattgcgat cgccaccatg cagattgagc tgagcacctg 2220
cttcttcctg tgcctgctga ggttctgctt ctctgccacc aggagatact acctgggggc 2280
tgtggagctg agctgggact acatgcagtc tgacctgggg gagctgcctg tggatgccag 2340
gttccccccc agagtgccca agagcttccc cttcaacacc tctgtggtgt acaagaagac 2400
cctgtttgtg gagttcactg accacctgtt caacattgcc aagcccaggc ccccctggat 2460
gggcctgctg ggccccacca tccaggctga ggtgtatgac actgtggtga tcaccctgaa 2520
gaacatggcc agccaccctg tgagcctgca tgctgtgggg gtgagctact ggaaggcctc 2580
tgagggggct gagtatgatg accagaccag ccagagggag aaggaggatg acaaggtgtt 2640
ccctgggggc agccacacct atgtgtggca ggtgctgaag gagaatggcc ccatggcctc 2700
tgaccccctg tgcctgacct acagctacct gagccatgtg gacctggtga aggacctgaa 2760
ctctggcctg attggggccc tgctggtgtg cagggagggc agcctggcca aggagaagac 2820
ccagaccctg cacaagttca tcctgctgtt tgctgtgttt gatgagggca agagctggca 2880
ctctgaaacc aagaacagcc tgatgcagga cagggatgct gcctctgcca gggcctggcc 2940
caagatgcac actgtgaatg gctatgtgaa caggagcctg cctggcctga ttggctgcca 3000
caggaagtct gtgtactggc atgtgattgg catgggcacc acccctgagg tgcacagcat 3060
cttcctggag ggccacacct tcctggtcag gaaccacagg caggccagcc tggagatcag 3120
ccccatcacc ttcctgactg cccagaccct gctgatggac ctgggccagt tcctgctgtt 3180
ctgccacatc agcagccacc agcatgatgg catggaggcc tatgtgaagg tggacagctg 3240
ccctgaggag ccccagctga ggatgaagaa caatgaggag gctgaggact atgatgatga 3300
cctgactgac tctgagatgg atgtggtgag gtttgatgat gacaacagcc ccagcttcat 3360
ccagatcagg tctgtggcca agaagcaccc caagacctgg gtgcactaca ttgctgctga 3420
ggaggaggac tgggactatg cccccctggt gctggcccct gatgacagga gctacaagag 3480
ccagtacctg aacaatggcc cccagaggat tggcaggaag tacaagaagg tcaggttcat 3540
ggcctacact gatgaaacct tcaagaccag ggaggccatc cagcatgagt ctggcatcct 3600
gggccccctg ctgtatgggg aggtggggga caccctgctg atcatcttca agaaccaggc 3660
cagcaggccc tacaacatct acccccatgg catcactgat gtgaggcccc tgtacagcag 3720
gaggctgccc aagggggtga agcacctgaa ggacttcccc atcctgcctg gggagatctt 3780
caagtacaag tggactgtga ctgtggagga tggccccacc aagtctgacc ccaggtgcct 3840
gaccagatac tacagcagct ttgtgaacat ggagagggac ctggcctctg gcctgattgg 3900
ccccctgctg atctgctaca aggagtctgt ggaccagagg ggcaaccaga tcatgtctga 3960
caagaggaat gtgatcctgt tctctgtgtt tgatgagaac aggagctggt acctgactga 4020
gaacatccag aggttcctgc ccaaccctgc tggggtgcag ctggaggacc ctgagttcca 4080
ggccagcaac atcatgcaca gcatcaatgg ctatgtgttt gacagcctgc agctgtctgt 4140
gtgcctgcat gaggtggcct actggtacat cctgagcatt ggggcccaga ctgacttcct 4200
gtctgtgttc ttctctggct acaccttcaa gcacaagatg gtgtatgagg acaccctgac 4260
cctgttcccc ttctctgggg agactgtgtt catgagcatg gagaaccctg gcctgtggat 4320
tctgggctgc cacaactctg acttcaggaa caggggcatg actgccctgc tgaaagtctc 4380
cagctgtgac aagaacactg gggactacta tgaggacagc tatgaggaca tctctgccta 4440
cctgctgagc aagaacaatg ccattgagcc caggagcttc agccagaacc ccccagtgct 4500
gaagaggcac cagagggaga tcaccaggac caccctgcag tctgaccagg aggagattga 4560
ctatgatgac accatctctg tggagatgaa gaaggaggac tttgacatct acgacgagga 4620
cgagaaccag agccccagga gcttccagaa gaagaccagg cactacttca ttgctgctgt 4680
ggagaggctg tgggactatg gcatgagcag cagcccccat gtgctgagga acagggccca 4740
gtctggctct gtgccccagt tcaagaaggt ggtgttccag gagttcactg atggcagctt 4800
cacccagccc ctgtacagag gggagctgaa tgagcacctg ggcctgctgg gcccctacat 4860
cagggctgag gtggaggaca acatcatggt gaccttcagg aaccaggcca gcaggcccta 4920
cagcttctac agcagcctga tcagctatga ggaggaccag aggcaggggg ctgagcccag 4980
gaagaacttt gtgaagccca atgaaaccaa gacctacttc tggaaggtgc agcaccacat 5040
ggcccccacc aaggatgagt ttgactgcaa ggcctgggcc tacttctctg atgtggacct 5100
ggagaaggat gtgcactctg gcctgattgg ccccctgctg gtgtgccaca ccaacaccct 5160
gaaccctgcc catggcaggc aggtgactgt gcaggagttt gccctgttct tcaccatctt 5220
tgatgaaacc aagagctggt acttcactga gaacatggag aggaactgca gggccccctg 5280
caacatccag atggaggacc ccaccttcaa ggagaactac aggttccatg ccatcaatgg 5340
ctacatcatg gacaccctgc ctggcctggt gatggcccag gaccagagga tcaggtggta 5400
cctgctgagc atgggcagca atgagaacat ccacagcatc cacttctctg gccatgtgtt 5460
cactgtgagg aagaaggagg agtacaagat ggccctgtac aacctgtacc ctggggtgtt 5520
tgagactgtg gagatgctgc ccagcaaggc tggcatctgg agggtggagt gcctgattgg 5580
ggagcacctg catgctggca tgagcaccct gttcctggtg tacagcaaca agtgccagac 5640
ccccctgggc atggcctctg gccacatcag ggacttccag atcactgcct ctggccagta 5700
tggccagtgg gcccccaagc tggccaggct gcactactct ggcagcatca atgcctggag 5760
caccaaggag cccttcagct ggatcaaggt ggacctgctg gcccccatga tcatccatgg 5820
catcaagacc cagggggcca ggcagaagtt cagcagcctg tacatcagcc agttcatcat 5880
catgtacagc ctggatggca agaagtggca gacctacagg ggcaacagca ctggcaccct 5940
gatggtgttc tttggcaatg tggacagctc tggcatcaag cacaacatct tcaacccccc 6000
catcattgcc agatacatca ggctgcaccc cacccactac agcatcagga gcaccctgag 6060
gatggagctg atgggctgtg acctgaacag ctgcagcatg cccctgggca tggagagcaa 6120
ggccatctct gatgcccaga tcactgccag cagctacttc accaacatgt ttgccacctg 6180
gagccccagc aaggccaggc tgcacctgca gggcaggagc aatgcctgga ggccccaggt 6240
caacaacccc aaggagtggc tgcaggtgga cttccagaag accatgaagg tgactggggt 6300
gaccacccag ggggtgaaga gcctgctgac cagcatgtat gtgaaggagt tcctgatcag 6360
cagcagccag gatggccacc agtggaccct gttcttccag aatggcaagg tgaaggtgtt 6420
ccagggcaac caggacagct tcacccctgt ggtgaacagc ctggaccccc ccctgctgac 6480
cagatacctg aggattcacc cccagagctg ggtgcaccag attgccctga ggatggaggt 6540
gctgggctgt gaggcccagg acctgtactg acctcgagct gtgccttcta gttgccagcc 6600
atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 6660
cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 6720
ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 6780
tggggatgcg gtgggctcta tggaccggtg cggccgcagg aacccctagt gatggagttg 6840
gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 6900
cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 6960
aa 6962
<210> 18
<211> 6289
<212> DNA
<213> 腺相关病毒2
<400> 18
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgta ggctcagagg cacacaggag 180
tttctgggct caccctgccc ccttccaacc cctcagttcc catcctccag cagctgtttg 240
tgtgctgcct ctgaagtcca cactgaacaa acttcagcct actcatgtcc ctaaaatggg 300
caaacattgc aagcagcaaa cagcaaacac acagccctcc ctgcctgctg accttggagc 360
tggggcagag gtcagagacc tctctgggcc catgccacct ccaacatcca ctcgacccct 420
tggaatttcg gtggagagga gcagaggttg tcctggcgtg gtttaggtag tgtgagaggg 480
gtcgacaggc tcagaggcac acaggagttt ctgggctcac cctgccccct tccaacccct 540
cagttcccat cctccagcag ctgtttgtgt gctgcctctg aagtccacac tgaacaaact 600
tcagcctact catgtcccta aaatgggcaa acattgcaag cagcaaacag caaacacaca 660
gccctccctg cctgctgacc ttggagctgg ggcagaggtc agagacctct ctgggcccat 720
gccacctcca acatccactc gaccccttgg aatttcggtg gagaggagca gaggttgtcc 780
tggcgtggtt taggtagtgt gagaggggtc gacgttaatt tttaaaaagc agtcaaaagt 840
ccaagtggcc cttggcagca tttactctct ctgtttgctc tggttaataa tctcaggagc 900
acaaacattc ctggaggcag gagaagaaat caacatcctg gacttatcct ctgggcctgt 960
taatttttaa aaagcagtca aaagtccaag tggcccttgg cagcatttac tctctctgtt 1020
tgctctggtt aataatctca ggagcacaaa cattcctgga ggcaggagaa gaaatcaaca 1080
tcctggactt atcctctggg cctagtcgac tggacacagg acgctgtggt ttctgagcca 1140
gggggcgact cagatcccag ccagtggact tagcccctgt ttgctcctcc gataactggg 1200
gtgaccttgg ttaatattca ccagcagcct cccccgttgc ccctctggat ccactgctta 1260
aatacggacg aggacagggc cctgtctcct cagcttcagg caccaccact gacctgggac 1320
agtgaatcgt aagtactagc agctacaatc cagctaccat tctgctttta ttttatggtt 1380
gggataaggc tggattattc tgagtccaag ctaggccctt ttgctaatca tgttcatacc 1440
tcttatcttc ctcccacagc tcctgggcaa cgtgctggtc tgtgtgctgg cccatcactt 1500
tggcaaagaa ttgcgatcgc caccatgcag attgagctga gcacctgctt cttcctgtgc 1560
ctgctgaggt tctgcttctc tgccaccagg agatactacc tgggggctgt ggagctgagc 1620
tgggactaca tgcagtctga cctgggggag ctgcctgtgg atgccaggtt cccccccaga 1680
gtgcccaaga gcttcccctt caacacctct gtggtgtaca agaagaccct gtttgtggag 1740
ttcactgacc acctgttcaa cattgccaag cccaggcccc cctggatggg cctgctgggc 1800
cccaccatcc aggctgaggt gtatgacact gtggtgatca ccctgaagaa catggccagc 1860
caccctgtga gcctgcatgc tgtgggggtg agctactgga aggcctctga gggggctgag 1920
tatgatgacc agaccagcca gagggagaag gaggatgaca aggtgttccc tgggggcagc 1980
cacacctatg tgtggcaggt gctgaaggag aatggcccca tggcctctga ccccctgtgc 2040
ctgacctaca gctacctgag ccatgtggac ctggtgaagg acctgaactc tggcctgatt 2100
ggggccctgc tggtgtgcag ggagggcagc ctggccaagg agaagaccca gaccctgcac 2160
aagttcatcc tgctgtttgc tgtgtttgat gagggcaaga gctggcactc tgaaaccaag 2220
aacagcctga tgcaggacag ggatgctgcc tctgccaggg cctggcccaa gatgcacact 2280
gtgaatggct atgtgaacag gagcctgcct ggcctgattg gctgccacag gaagtctgtg 2340
tactggcatg tgattggcat gggcaccacc cctgaggtgc acagcatctt cctggagggc 2400
cacaccttcc tggtcaggaa ccacaggcag gccagcctgg agatcagccc catcaccttc 2460
ctgactgccc agaccctgct gatggacctg ggccagttcc tgctgttctg ccacatcagc 2520
agccaccagc atgatggcat ggaggcctat gtgaaggtgg acagctgccc tgaggagccc 2580
cagctgagga tgaagaacaa tgaggaggct gaggactatg atgatgacct gactgactct 2640
gagatggatg tggtgaggtt tgatgatgac aacagcccca gcttcatcca gatcaggtct 2700
gtggccaaga agcaccccaa gacctgggtg cactacattg ctgctgagga ggaggactgg 2760
gactatgccc ccctggtgct ggcccctgat gacaggagct acaagagcca gtacctgaac 2820
aatggccccc agaggattgg caggaagtac aagaaggtca ggttcatggc ctacactgat 2880
gaaaccttca agaccaggga ggccatccag catgagtctg gcatcctggg ccccctgctg 2940
tatggggagg tgggggacac cctgctgatc atcttcaaga accaggccag caggccctac 3000
aacatctacc cccatggcat cactgatgtg aggcccctgt acagcaggag gctgcccaag 3060
ggggtgaagc acctgaagga cttccccatc ctgcctgggg agatcttcaa gtacaagtgg 3120
actgtgactg tggaggatgg ccccaccaag tctgacccca ggtgcctgac cagatactac 3180
agcagctttg tgaacatgga gagggacctg gcctctggcc tgattggccc cctgctgatc 3240
tgctacaagg agtctgtgga ccagaggggc aaccagatca tgtctgacaa gaggaatgtg 3300
atcctgttct ctgtgtttga tgagaacagg agctggtacc tgactgagaa catccagagg 3360
ttcctgccca accctgctgg ggtgcagctg gaggaccctg agttccaggc cagcaacatc 3420
atgcacagca tcaatggcta tgtgtttgac agcctgcagc tgtctgtgtg cctgcatgag 3480
gtggcctact ggtacatcct gagcattggg gcccagactg acttcctgtc tgtgttcttc 3540
tctggctaca ccttcaagca caagatggtg tatgaggaca ccctgaccct gttccccttc 3600
tctggggaga ctgtgttcat gagcatggag aaccctggcc tgtggattct gggctgccac 3660
aactctgact tcaggaacag gggcatgact gccctgctga aagtctccag ctgtgacaag 3720
aacactgggg actactatga ggacagctat gaggacatct ctgcctacct gctgagcaag 3780
aacaatgcca ttgagcccag gagcttcagc cagaaccccc cagtgctgaa gaggcaccag 3840
agggagatca ccaggaccac cctgcagtct gaccaggagg agattgacta tgatgacacc 3900
atctctgtgg agatgaagaa ggaggacttt gacatctacg acgaggacga gaaccagagc 3960
cccaggagct tccagaagaa gaccaggcac tacttcattg ctgctgtgga gaggctgtgg 4020
gactatggca tgagcagcag cccccatgtg ctgaggaaca gggcccagtc tggctctgtg 4080
ccccagttca agaaggtggt gttccaggag ttcactgatg gcagcttcac ccagcccctg 4140
tacagagggg agctgaatga gcacctgggc ctgctgggcc cctacatcag ggctgaggtg 4200
gaggacaaca tcatggtgac cttcaggaac caggccagca ggccctacag cttctacagc 4260
agcctgatca gctatgagga ggaccagagg cagggggctg agcccaggaa gaactttgtg 4320
aagcccaatg aaaccaagac ctacttctgg aaggtgcagc accacatggc ccccaccaag 4380
gatgagtttg actgcaaggc ctgggcctac ttctctgatg tggacctgga gaaggatgtg 4440
cactctggcc tgattggccc cctgctggtg tgccacacca acaccctgaa ccctgcccat 4500
ggcaggcagg tgactgtgca ggagtttgcc ctgttcttca ccatctttga tgaaaccaag 4560
agctggtact tcactgagaa catggagagg aactgcaggg ccccctgcaa catccagatg 4620
gaggacccca ccttcaagga gaactacagg ttccatgcca tcaatggcta catcatggac 4680
accctgcctg gcctggtgat ggcccaggac cagaggatca ggtggtacct gctgagcatg 4740
ggcagcaatg agaacatcca cagcatccac ttctctggcc atgtgttcac tgtgaggaag 4800
aaggaggagt acaagatggc cctgtacaac ctgtaccctg gggtgtttga gactgtggag 4860
atgctgccca gcaaggctgg catctggagg gtggagtgcc tgattgggga gcacctgcat 4920
gctggcatga gcaccctgtt cctggtgtac agcaacaagt gccagacccc cctgggcatg 4980
gcctctggcc acatcaggga cttccagatc actgcctctg gccagtatgg ccagtgggcc 5040
cccaagctgg ccaggctgca ctactctggc agcatcaatg cctggagcac caaggagccc 5100
ttcagctgga tcaaggtgga cctgctggcc cccatgatca tccatggcat caagacccag 5160
ggggccaggc agaagttcag cagcctgtac atcagccagt tcatcatcat gtacagcctg 5220
gatggcaaga agtggcagac ctacaggggc aacagcactg gcaccctgat ggtgttcttt 5280
ggcaatgtgg acagctctgg catcaagcac aacatcttca acccccccat cattgccaga 5340
tacatcaggc tgcaccccac ccactacagc atcaggagca ccctgaggat ggagctgatg 5400
ggctgtgacc tgaacagctg cagcatgccc ctgggcatgg agagcaaggc catctctgat 5460
gcccagatca ctgccagcag ctacttcacc aacatgtttg ccacctggag ccccagcaag 5520
gccaggctgc acctgcaggg caggagcaat gcctggaggc cccaggtcaa caaccccaag 5580
gagtggctgc aggtggactt ccagaagacc atgaaggtga ctggggtgac cacccagggg 5640
gtgaagagcc tgctgaccag catgtatgtg aaggagttcc tgatcagcag cagccaggat 5700
ggccaccagt ggaccctgtt cttccagaat ggcaaggtga aggtgttcca gggcaaccag 5760
gacagcttca cccctgtggt gaacagcctg gacccccccc tgctgaccag atacctgagg 5820
attcaccccc agagctgggt gcaccagatt gccctgagga tggaggtgct gggctgtgag 5880
gcccaggacc tgtactgacc tcgagctgtg ccttctagtt gccagccatc tgttgtttgc 5940
ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa 6000
aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg 6060
gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg 6120
ggctctatgg accggtgcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc 6180
tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg 6240
cccgggcggc ctcagtgagc gagcgagcgc gcagagaggg agtggccaa 6289
<210> 19
<211> 5430
<212> DNA
<213> 腺相关病毒2
<400> 19
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgacgt ggacttagcc 180
cctgtttgct cctccgataa ctggggtgac cttggttaat attcaccagc agcctccccg 240
tggacttagc ccctgtttgc tcctccgata actggggtga ccttggttaa tattcaccag 300
cagcctcccc acgcgaaacg tcgactggac acaggacgct gtggtttctg agccaggggg 360
cgactcagat cccagccagt ggacttagcc cctgtttgct cctccgataa ctggggtgac 420
cttggttaat attcaccagc agcctccccc gttgcccctc tggatccact gcttaaatac 480
ggacgaggac agggccctgt ctcctcagct tcaggcacca ccactgacct gggacagtga 540
atcgtaagta ctagcagcta caatccagct accattctgc ttttatttta tggttgggat 600
aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta 660
tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca 720
aagaattgcg atcgccacca tgcagattga gctgagcacc tgcttcttcc tgtgcctgct 780
gaggttctgc ttctctgcca ccaggagata ctacctgggg gctgtggagc tgagctggga 840
ctacatgcag tctgacctgg gggagctgcc tgtggatgcc aggttccccc ccagagtgcc 900
caagagcttc cccttcaaca cctctgtggt gtacaagaag accctgtttg tggagttcac 960
tgaccacctg ttcaacattg ccaagcccag gcccccctgg atgggcctgc tgggccccac 1020
catccaggct gaggtgtatg acactgtggt gatcaccctg aagaacatgg ccagccaccc 1080
tgtgagcctg catgctgtgg gggtgagcta ctggaaggcc tctgaggggg ctgagtatga 1140
tgaccagacc agccagaggg agaaggagga tgacaaggtg ttccctgggg gcagccacac 1200
ctatgtgtgg caggtgctga aggagaatgg ccccatggcc tctgaccccc tgtgcctgac 1260
ctacagctac ctgagccatg tggacctggt gaaggacctg aactctggcc tgattggggc 1320
cctgctggtg tgcagggagg gcagcctggc caaggagaag acccagaccc tgcacaagtt 1380
catcctgctg tttgctgtgt ttgatgaggg caagagctgg cactctgaaa ccaagaacag 1440
cctgatgcag gacagggatg ctgcctctgc cagggcctgg cccaagatgc acactgtgaa 1500
tggctatgtg aacaggagcc tgcctggcct gattggctgc cacaggaagt ctgtgtactg 1560
gcatgtgatt ggcatgggca ccacccctga ggtgcacagc atcttcctgg agggccacac 1620
cttcctggtc aggaaccaca ggcaggccag cctggagatc agccccatca ccttcctgac 1680
tgcccagacc ctgctgatgg acctgggcca gttcctgctg ttctgccaca tcagcagcca 1740
ccagcatgat ggcatggagg cctatgtgaa ggtggacagc tgccctgagg agccccagct 1800
gaggatgaag aacaatgagg aggctgagga ctatgatgat gacctgactg actctgagat 1860
ggatgtggtg aggtttgatg atgacaacag ccccagcttc atccagatca ggtctgtggc 1920
caagaagcac cccaagacct gggtgcacta cattgctgct gaggaggagg actgggacta 1980
tgcccccctg gtgctggccc ctgatgacag gagctacaag agccagtacc tgaacaatgg 2040
cccccagagg attggcagga agtacaagaa ggtcaggttc atggcctaca ctgatgaaac 2100
cttcaagacc agggaggcca tccagcatga gtctggcatc ctgggccccc tgctgtatgg 2160
ggaggtgggg gacaccctgc tgatcatctt caagaaccag gccagcaggc cctacaacat 2220
ctacccccat ggcatcactg atgtgaggcc cctgtacagc aggaggctgc ccaagggggt 2280
gaagcacctg aaggacttcc ccatcctgcc tggggagatc ttcaagtaca agtggactgt 2340
gactgtggag gatggcccca ccaagtctga ccccaggtgc ctgaccagat actacagcag 2400
ctttgtgaac atggagaggg acctggcctc tggcctgatt ggccccctgc tgatctgcta 2460
caaggagtct gtggaccaga ggggcaacca gatcatgtct gacaagagga atgtgatcct 2520
gttctctgtg tttgatgaga acaggagctg gtacctgact gagaacatcc agaggttcct 2580
gcccaaccct gctggggtgc agctggagga ccctgagttc caggccagca acatcatgca 2640
cagcatcaat ggctatgtgt ttgacagcct gcagctgtct gtgtgcctgc atgaggtggc 2700
ctactggtac atcctgagca ttggggccca gactgacttc ctgtctgtgt tcttctctgg 2760
ctacaccttc aagcacaaga tggtgtatga ggacaccctg accctgttcc ccttctctgg 2820
ggagactgtg ttcatgagca tggagaaccc tggcctgtgg attctgggct gccacaactc 2880
tgacttcagg aacaggggca tgactgccct gctgaaagtc tccagctgtg acaagaacac 2940
tggggactac tatgaggaca gctatgagga catctctgcc tacctgctga gcaagaacaa 3000
tgccattgag cccaggagct tcagccagaa ccccccagtg ctgaagaggc accagaggga 3060
gatcaccagg accaccctgc agtctgacca ggaggagatt gactatgatg acaccatctc 3120
tgtggagatg aagaaggagg actttgacat ctacgacgag gacgagaacc agagccccag 3180
gagcttccag aagaagacca ggcactactt cattgctgct gtggagaggc tgtgggacta 3240
tggcatgagc agcagccccc atgtgctgag gaacagggcc cagtctggct ctgtgcccca 3300
gttcaagaag gtggtgttcc aggagttcac tgatggcagc ttcacccagc ccctgtacag 3360
aggggagctg aatgagcacc tgggcctgct gggcccctac atcagggctg aggtggagga 3420
caacatcatg gtgaccttca ggaaccaggc cagcaggccc tacagcttct acagcagcct 3480
gatcagctat gaggaggacc agaggcaggg ggctgagccc aggaagaact ttgtgaagcc 3540
caatgaaacc aagacctact tctggaaggt gcagcaccac atggccccca ccaaggatga 3600
gtttgactgc aaggcctggg cctacttctc tgatgtggac ctggagaagg atgtgcactc 3660
tggcctgatt ggccccctgc tggtgtgcca caccaacacc ctgaaccctg cccatggcag 3720
gcaggtgact gtgcaggagt ttgccctgtt cttcaccatc tttgatgaaa ccaagagctg 3780
gtacttcact gagaacatgg agaggaactg cagggccccc tgcaacatcc agatggagga 3840
ccccaccttc aaggagaact acaggttcca tgccatcaat ggctacatca tggacaccct 3900
gcctggcctg gtgatggccc aggaccagag gatcaggtgg tacctgctga gcatgggcag 3960
caatgagaac atccacagca tccacttctc tggccatgtg ttcactgtga ggaagaagga 4020
ggagtacaag atggccctgt acaacctgta ccctggggtg tttgagactg tggagatgct 4080
gcccagcaag gctggcatct ggagggtgga gtgcctgatt ggggagcacc tgcatgctgg 4140
catgagcacc ctgttcctgg tgtacagcaa caagtgccag acccccctgg gcatggcctc 4200
tggccacatc agggacttcc agatcactgc ctctggccag tatggccagt gggcccccaa 4260
gctggccagg ctgcactact ctggcagcat caatgcctgg agcaccaagg agcccttcag 4320
ctggatcaag gtggacctgc tggcccccat gatcatccat ggcatcaaga cccagggggc 4380
caggcagaag ttcagcagcc tgtacatcag ccagttcatc atcatgtaca gcctggatgg 4440
caagaagtgg cagacctaca ggggcaacag cactggcacc ctgatggtgt tctttggcaa 4500
tgtggacagc tctggcatca agcacaacat cttcaacccc cccatcattg ccagatacat 4560
caggctgcac cccacccact acagcatcag gagcaccctg aggatggagc tgatgggctg 4620
tgacctgaac agctgcagca tgcccctggg catggagagc aaggccatct ctgatgccca 4680
gatcactgcc agcagctact tcaccaacat gtttgccacc tggagcccca gcaaggccag 4740
gctgcacctg cagggcagga gcaatgcctg gaggccccag gtcaacaacc ccaaggagtg 4800
gctgcaggtg gacttccaga agaccatgaa ggtgactggg gtgaccaccc agggggtgaa 4860
gagcctgctg accagcatgt atgtgaagga gttcctgatc agcagcagcc aggatggcca 4920
ccagtggacc ctgttcttcc agaatggcaa ggtgaaggtg ttccagggca accaggacag 4980
cttcacccct gtggtgaaca gcctggaccc ccccctgctg accagatacc tgaggattca 5040
cccccagagc tgggtgcacc agattgccct gaggatggag gtgctgggct gtgaggccca 5100
ggacctgtac tgacctcgag gcactgtcct ttcctaataa aatgaggaaa ttgcatcgca 5160
ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 5220
ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg gcacgtggcg 5280
gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac 5340
tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag 5400
cgagcgagcg cgcagagagg gagtggccaa 5430
<210> 20
<211> 5779
<212> DNA
<213> 腺相关病毒2
<400> 20
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgacgt ggacttagcc 180
cctgtttgct cctccgataa ctggggtgac cttggttaat attcaccagc agcctccccg 240
tggacttagc ccctgtttgc tcctccgata actggggtga ccttggttaa tattcaccag 300
cagcctcccc acgcgaaacg tcgactggac acaggacgct gtggtttctg agccaggggg 360
cgactcagat cccagccagt ggacttagcc cctgtttgct cctccgataa ctggggtgac 420
cttggttaat attcaccagc agcctccccc gttgcccctc tggatccact gcttaaatac 480
ggacgaggac agggccctgt ctcctcagct tcaggcacca ccactgacct gggacagtga 540
atcgtaagta ctagcagcta caatccagct accattctgc ttttatttta tggttgggat 600
aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta 660
tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca 720
aagaattgcg atcgccacca tgcagattga gctgagcacc tgcttcttcc tgtgcctgct 780
gaggttctgc ttctctgcca ccaggagata ctacctgggg gctgtggagc tgagctggga 840
ctacatgcag tctgacctgg gggagctgcc tgtggatgcc aggttccccc ccagagtgcc 900
caagagcttc cccttcaaca cctctgtggt gtacaagaag accctgtttg tggagttcac 960
tgaccacctg ttcaacattg ccaagcccag gcccccctgg atgggcctgc tgggccccac 1020
catccaggct gaggtgtatg acactgtggt gatcaccctg aagaacatgg ccagccaccc 1080
tgtgagcctg catgctgtgg gggtgagcta ctggaaggcc tctgaggggg ctgagtatga 1140
tgaccagacc agccagaggg agaaggagga tgacaaggtg ttccctgggg gcagccacac 1200
ctatgtgtgg caggtgctga aggagaatgg ccccatggcc tctgaccccc tgtgcctgac 1260
ctacagctac ctgagccatg tggacctggt gaaggacctg aactctggcc tgattggggc 1320
cctgctggtg tgcagggagg gcagcctggc caaggagaag acccagaccc tgcacaagtt 1380
catcctgctg tttgctgtgt ttgatgaggg caagagctgg cactctgaaa ccaagaacag 1440
cctgatgcag gacagggatg ctgcctctgc cagggcctgg cccaagatgc acactgtgaa 1500
tggctatgtg aacaggagcc tgcctggcct gattggctgc cacaggaagt ctgtgtactg 1560
gcatgtgatt ggcatgggca ccacccctga ggtgcacagc atcttcctgg agggccacac 1620
cttcctggtc aggaaccaca ggcaggccag cctggagatc agccccatca ccttcctgac 1680
tgcccagacc ctgctgatgg acctgggcca gttcctgctg ttctgccaca tcagcagcca 1740
ccagcatgat ggcatggagg cctatgtgaa ggtggacagc tgccctgagg agccccagct 1800
gaggatgaag aacaatgagg aggctgagga ctatgatgat gacctgactg actctgagat 1860
ggatgtggtg aggtttgatg atgacaacag ccccagcttc atccagatca ggtctgtggc 1920
caagaagcac cccaagacct gggtgcacta cattgctgct gaggaggagg actgggacta 1980
tgcccccctg gtgctggccc ctgatgacag gagctacaag agccagtacc tgaacaatgg 2040
cccccagagg attggcagga agtacaagaa ggtcaggttc atggcctaca ctgatgaaac 2100
cttcaagacc agggaggcca tccagcatga gtctggcatc ctgggccccc tgctgtatgg 2160
ggaggtgggg gacaccctgc tgatcatctt caagaaccag gccagcaggc cctacaacat 2220
ctacccccat ggcatcactg atgtgaggcc cctgtacagc aggaggctgc ccaagggggt 2280
gaagcacctg aaggacttcc ccatcctgcc tggggagatc ttcaagtaca agtggactgt 2340
gactgtggag gatggcccca ccaagtctga ccccaggtgc ctgaccagat actacagcag 2400
ctttgtgaac atggagaggg acctggcctc tggcctgatt ggccccctgc tgatctgcta 2460
caaggagtct gtggaccaga ggggcaacca gatcatgtct gacaagagga atgtgatcct 2520
gttctctgtg tttgatgaga acaggagctg gtacctgact gagaacatcc agaggttcct 2580
gcccaaccct gctggggtgc agctggagga ccctgagttc caggccagca acatcatgca 2640
cagcatcaat ggctatgtgt ttgacagcct gcagctgtct gtgtgcctgc atgaggtggc 2700
ctactggtac atcctgagca ttggggccca gactgacttc ctgtctgtgt tcttctctgg 2760
ctacaccttc aagcacaaga tggtgtatga ggacaccctg accctgttcc ccttctctgg 2820
ggagactgtg ttcatgagca tggagaaccc tggcctgtgg attctgggct gccacaactc 2880
tgacttcagg aacaggggca tgactgccct gctgaaagtc tccagctgtg acaagaacac 2940
tggggactac tatgaggaca gctatgagga catctctgcc tacctgctga gcaagaacaa 3000
tgccattgag cccaggagct tcagccagaa ccccccagtg ctgaagaggc accagaggga 3060
gatcaccagg accaccctgc agtctgacca ggaggagatt gactatgatg acaccatctc 3120
tgtggagatg aagaaggagg actttgacat ctacgacgag gacgagaacc agagccccag 3180
gagcttccag aagaagacca ggcactactt cattgctgct gtggagaggc tgtgggacta 3240
tggcatgagc agcagccccc atgtgctgag gaacagggcc cagtctggct ctgtgcccca 3300
gttcaagaag gtggtgttcc aggagttcac tgatggcagc ttcacccagc ccctgtacag 3360
aggggagctg aatgagcacc tgggcctgct gggcccctac atcagggctg aggtggagga 3420
caacatcatg gtgaccttca ggaaccaggc cagcaggccc tacagcttct acagcagcct 3480
gatcagctat gaggaggacc agaggcaggg ggctgagccc aggaagaact ttgtgaagcc 3540
caatgaaacc aagacctact tctggaaggt gcagcaccac atggccccca ccaaggatga 3600
gtttgactgc aaggcctggg cctacttctc tgatgtggac ctggagaagg atgtgcactc 3660
tggcctgatt ggccccctgc tggtgtgcca caccaacacc ctgaaccctg cccatggcag 3720
gcaggtgact gtgcaggagt ttgccctgtt cttcaccatc tttgatgaaa ccaagagctg 3780
gtacttcact gagaacatgg agaggaactg cagggccccc tgcaacatcc agatggagga 3840
ccccaccttc aaggagaact acaggttcca tgccatcaat ggctacatca tggacaccct 3900
gcctggcctg gtgatggccc aggaccagag gatcaggtgg tacctgctga gcatgggcag 3960
caatgagaac atccacagca tccacttctc tggccatgtg ttcactgtga ggaagaagga 4020
ggagtacaag atggccctgt acaacctgta ccctggggtg tttgagactg tggagatgct 4080
gcccagcaag gctggcatct ggagggtgga gtgcctgatt ggggagcacc tgcatgctgg 4140
catgagcacc ctgttcctgg tgtacagcaa caagtgccag acccccctgg gcatggcctc 4200
tggccacatc agggacttcc agatcactgc ctctggccag tatggccagt gggcccccaa 4260
gctggccagg ctgcactact ctggcagcat caatgcctgg agcaccaagg agcccttcag 4320
ctggatcaag gtggacctgc tggcccccat gatcatccat ggcatcaaga cccagggggc 4380
caggcagaag ttcagcagcc tgtacatcag ccagttcatc atcatgtaca gcctggatgg 4440
caagaagtgg cagacctaca ggggcaacag cactggcacc ctgatggtgt tctttggcaa 4500
tgtggacagc tctggcatca agcacaacat cttcaacccc cccatcattg ccagatacat 4560
caggctgcac cccacccact acagcatcag gagcaccctg aggatggagc tgatgggctg 4620
tgacctgaac agctgcagca tgcccctggg catggagagc aaggccatct ctgatgccca 4680
gatcactgcc agcagctact tcaccaacat gtttgccacc tggagcccca gcaaggccag 4740
gctgcacctg cagggcagga gcaatgcctg gaggccccag gtcaacaacc ccaaggagtg 4800
gctgcaggtg gacttccaga agaccatgaa ggtgactggg gtgaccaccc agggggtgaa 4860
gagcctgctg accagcatgt atgtgaagga gttcctgatc agcagcagcc aggatggcca 4920
ccagtggacc ctgttcttcc agaatggcaa ggtgaaggtg ttccagggca accaggacag 4980
cttcacccct gtggtgaaca gcctggaccc ccccctgctg accagatacc tgaggattca 5040
cccccagagc tgggtgcacc agattgccct gaggatggag gtgctgggct gtgaggccca 5100
ggacctgtac tgacctcgag gcactgtcct ttcctaataa aatgaggaaa ttgcatcgca 5160
ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga 5220
ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg gcactcgaca 5280
ggttaatttt taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct 5340
gtttgctctg gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca 5400
acatcctgga cttatcctct gggcctctcc ccacccccag gagaggctca ggttaatttt 5460
taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct gtttgctctg 5520
gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca acatcctgga 5580
cttatcctct gggcctctcc ccacccccag gagaggctgt cgagtggcgg ccgcaggaac 5640
ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc 5700
gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc 5760
gcagagaggg agtggccaa 5779
<210> 21
<211> 5962
<212> DNA
<213> 腺相关病毒2
<400> 21
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgtaa gtactagcag ctacaatcca gctaccattc tgcttttatt ttatggttgg 780
gataaggctg gattattctg agtccaagct aggccctttt gctaatcatg ttcatacctc 840
ttatcttcct cccacagctc ctgggcaacg tgctggtctg tgtgctggcc catcactttg 900
gcaaagaatt gcgatcgcca ccatgcagat tgagctgagc acctgcttct tcctgtgcct 960
gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg agctgagctg 1020
ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc cccccagagt 1080
gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt ttgtggagtt 1140
cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc tgctgggccc 1200
caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca tggccagcca 1260
ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg gggctgagta 1320
tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg ggggcagcca 1380
cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc ccctgtgcct 1440
gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg gcctgattgg 1500
ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga ccctgcacaa 1560
gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg aaaccaagaa 1620
cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga tgcacactgt 1680
gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga agtctgtgta 1740
ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc tggagggcca 1800
caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca tcaccttcct 1860
gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc acatcagcag 1920
ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg aggagcccca 1980
gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga ctgactctga 2040
gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga tcaggtctgt 2100
ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg aggactggga 2160
ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt acctgaacaa 2220
tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct acactgatga 2280
aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc ccctgctgta 2340
tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca ggccctacaa 2400
catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc tgcccaaggg 2460
ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt acaagtggac 2520
tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca gatactacag 2580
cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc tgctgatctg 2640
ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga ggaatgtgat 2700
cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca tccagaggtt 2760
cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca gcaacatcat 2820
gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc tgcatgaggt 2880
ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg tgttcttctc 2940
tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt tccccttctc 3000
tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg gctgccacaa 3060
ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct gtgacaagaa 3120
cactggggac tactatgagg acagctatga ggacatctct gcctacctgc tgagcaagaa 3180
caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga ggcaccagag 3240
ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg atgacaccat 3300
ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga accagagccc 3360
caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga ggctgtggga 3420
ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg gctctgtgcc 3480
ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc agcccctgta 3540
cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg ctgaggtgga 3600
ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct tctacagcag 3660
cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga actttgtgaa 3720
gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc ccaccaagga 3780
tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga aggatgtgca 3840
ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc ctgcccatgg 3900
caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg aaaccaagag 3960
ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca tccagatgga 4020
ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca tcatggacac 4080
cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc tgagcatggg 4140
cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg tgaggaagaa 4200
ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga ctgtggagat 4260
gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc acctgcatgc 4320
tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc tgggcatggc 4380
ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc agtgggcccc 4440
caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca aggagccctt 4500
cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca agacccaggg 4560
ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt acagcctgga 4620
tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg tgttctttgg 4680
caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca ttgccagata 4740
catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg agctgatggg 4800
ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca tctctgatgc 4860
ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc ccagcaaggc 4920
caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca accccaagga 4980
gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca cccagggggt 5040
gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca gccaggatgg 5100
ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg gcaaccagga 5160
cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat acctgaggat 5220
tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg gctgtgaggc 5280
ccaggacctg tactgacctc gaggcactgt cctttcctaa taaaatgagg aaattgcatc 5340
gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 5400
ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggcactcg 5460
acaggttaat ttttaaaaag cagtcaaaag tccaagtggc ccttggcagc atttactctc 5520
tctgtttgct ctggttaata atctcaggag cacaaacatt cctggaggca ggagaagaaa 5580
tcaacatcct ggacttatcc tctgggcctc tccccacccc caggagaggc tcaggttaat 5640
ttttaaaaag cagtcaaaag tccaagtggc ccttggcagc atttactctc tctgtttgct 5700
ctggttaata atctcaggag cacaaacatt cctggaggca ggagaagaaa tcaacatcct 5760
ggacttatcc tctgggcctc tccccacccc caggagaggc tgtcgagtgg cggccgcagg 5820
aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 5880
ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 5940
cgcgcagaga gggagtggcc aa 5962
<210> 22
<211> 5919
<212> DNA
<213> 腺相关病毒2
<400> 22
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgta ggctcagagg cacacaggag 180
tttctgggct caccctgccc ccttccaacc cctcagttcc catcctccag cagctgtttg 240
tgtgctgcct ctgaagtcca cactgaacaa acttcagcct actcatgtcc ctaaaatggg 300
caaacattgc aagcagcaaa cagcaaacac acagccctcc ctgcctgctg accttggagc 360
tggggcagag gtcagagacc tctctgggcc catgccacct ccaacatcca ctcgacccct 420
tggaatttcg gtggagagga gcagaggttg tcctggcgtg gtttaggtag tgtgagaggg 480
gtcgacgatc ttgctaccag tggaacagcc actaaggatt ctgcagtgag agcagagggc 540
cagctaagtg gtactctccc agagactgtc tgactcacgc caccccctcc accttggaca 600
caggacgctg tggtttctga gccaggtaca atgactcctt tcggtaagtg cagtggaagc 660
tgtacactgc ccaggcaaag cgtccgggca gcgtaggcgg gcgactcaga tcccagccag 720
tggacttagc ccctgtttgc tcctccgata actggggtga ccttggttaa tattcaccag 780
cagcctcccc cgttgcccct ctggatccac tgcttaaata cggacgagga cagggccctg 840
tctcctcagc ttcaggcacc accactgacc tgggacagtg aatcgtaagt atgcctttca 900
ctgcgagagg ttctggagag gcttctgagc tccccatggc ccaggcaggc agcaggtctg 960
gggcaggagg ggggttgtgg agtgggtatc cgcctgctga ggtgcagggc agatcatcat 1020
gtgccttgac tcggggcctg gcccccccat ctctgtcttg caggacaatt gccgtcttct 1080
gtctcgtggg gcatcctcct gctggcaggc ctgtgctgcc tggtccctgt ctccctggct 1140
gaggaccggc caccatgcag attgagctga gcacctgctt cttcctgtgc ctgctgaggt 1200
tctgcttctc tgccaccagg agatactacc tgggggctgt ggagctgagc tgggactaca 1260
tgcagtctga cctgggggag ctgcctgtgg atgccaggtt cccccccaga gtgcccaaga 1320
gcttcccctt caacacctct gtggtgtaca agaagaccct gtttgtggag ttcactgacc 1380
acctgttcaa cattgccaag cccaggcccc cctggatggg cctgctgggc cccaccatcc 1440
aggctgaggt gtatgacact gtggtgatca ccctgaagaa catggccagc caccctgtga 1500
gcctgcatgc tgtgggggtg agctactgga aggcctctga gggggctgag tatgatgacc 1560
agaccagcca gagggagaag gaggatgaca aggtgttccc tgggggcagc cacacctatg 1620
tgtggcaggt gctgaaggag aatggcccca tggcctctga ccccctgtgc ctgacctaca 1680
gctacctgag ccatgtggac ctggtgaagg acctgaactc tggcctgatt ggggccctgc 1740
tggtgtgcag ggagggcagc ctggccaagg agaagaccca gaccctgcac aagttcatcc 1800
tgctgtttgc tgtgtttgat gagggcaaga gctggcactc tgaaaccaag aacagcctga 1860
tgcaggacag ggatgctgcc tctgccaggg cctggcccaa gatgcacact gtgaatggct 1920
atgtgaacag gagcctgcct ggcctgattg gctgccacag gaagtctgtg tactggcatg 1980
tgattggcat gggcaccacc cctgaggtgc acagcatctt cctggagggc cacaccttcc 2040
tggtcaggaa ccacaggcag gccagcctgg agatcagccc catcaccttc ctgactgccc 2100
agaccctgct gatggacctg ggccagttcc tgctgttctg ccacatcagc agccaccagc 2160
atgatggcat ggaggcctat gtgaaggtgg acagctgccc tgaggagccc cagctgagga 2220
tgaagaacaa tgaggaggct gaggactatg atgatgacct gactgactct gagatggatg 2280
tggtgaggtt tgatgatgac aacagcccca gcttcatcca gatcaggtct gtggccaaga 2340
agcaccccaa gacctgggtg cactacattg ctgctgagga ggaggactgg gactatgccc 2400
ccctggtgct ggcccctgat gacaggagct acaagagcca gtacctgaac aatggccccc 2460
agaggattgg caggaagtac aagaaggtca ggttcatggc ctacactgat gaaaccttca 2520
agaccaggga ggccatccag catgagtctg gcatcctggg ccccctgctg tatggggagg 2580
tgggggacac cctgctgatc atcttcaaga accaggccag caggccctac aacatctacc 2640
cccatggcat cactgatgtg aggcccctgt acagcaggag gctgcccaag ggggtgaagc 2700
acctgaagga cttccccatc ctgcctgggg agatcttcaa gtacaagtgg actgtgactg 2760
tggaggatgg ccccaccaag tctgacccca ggtgcctgac cagatactac agcagctttg 2820
tgaacatgga gagggacctg gcctctggcc tgattggccc cctgctgatc tgctacaagg 2880
agtctgtgga ccagaggggc aaccagatca tgtctgacaa gaggaatgtg atcctgttct 2940
ctgtgtttga tgagaacagg agctggtacc tgactgagaa catccagagg ttcctgccca 3000
accctgctgg ggtgcagctg gaggaccctg agttccaggc cagcaacatc atgcacagca 3060
tcaatggcta tgtgtttgac agcctgcagc tgtctgtgtg cctgcatgag gtggcctact 3120
ggtacatcct gagcattggg gcccagactg acttcctgtc tgtgttcttc tctggctaca 3180
ccttcaagca caagatggtg tatgaggaca ccctgaccct gttccccttc tctggggaga 3240
ctgtgttcat gagcatggag aaccctggcc tgtggattct gggctgccac aactctgact 3300
tcaggaacag gggcatgact gccctgctga aagtctccag ctgtgacaag aacactgggg 3360
actactatga ggacagctat gaggacatct ctgcctacct gctgagcaag aacaatgcca 3420
ttgagcccag gagcttcagc cagaaccccc cagtgctgaa gaggcaccag agggagatca 3480
ccaggaccac cctgcagtct gaccaggagg agattgacta tgatgacacc atctctgtgg 3540
agatgaagaa ggaggacttt gacatctacg acgaggacga gaaccagagc cccaggagct 3600
tccagaagaa gaccaggcac tacttcattg ctgctgtgga gaggctgtgg gactatggca 3660
tgagcagcag cccccatgtg ctgaggaaca gggcccagtc tggctctgtg ccccagttca 3720
agaaggtggt gttccaggag ttcactgatg gcagcttcac ccagcccctg tacagagggg 3780
agctgaatga gcacctgggc ctgctgggcc cctacatcag ggctgaggtg gaggacaaca 3840
tcatggtgac cttcaggaac caggccagca ggccctacag cttctacagc agcctgatca 3900
gctatgagga ggaccagagg cagggggctg agcccaggaa gaactttgtg aagcccaatg 3960
aaaccaagac ctacttctgg aaggtgcagc accacatggc ccccaccaag gatgagtttg 4020
actgcaaggc ctgggcctac ttctctgatg tggacctgga gaaggatgtg cactctggcc 4080
tgattggccc cctgctggtg tgccacacca acaccctgaa ccctgcccat ggcaggcagg 4140
tgactgtgca ggagtttgcc ctgttcttca ccatctttga tgaaaccaag agctggtact 4200
tcactgagaa catggagagg aactgcaggg ccccctgcaa catccagatg gaggacccca 4260
ccttcaagga gaactacagg ttccatgcca tcaatggcta catcatggac accctgcctg 4320
gcctggtgat ggcccaggac cagaggatca ggtggtacct gctgagcatg ggcagcaatg 4380
agaacatcca cagcatccac ttctctggcc atgtgttcac tgtgaggaag aaggaggagt 4440
acaagatggc cctgtacaac ctgtaccctg gggtgtttga gactgtggag atgctgccca 4500
gcaaggctgg catctggagg gtggagtgcc tgattgggga gcacctgcat gctggcatga 4560
gcaccctgtt cctggtgtac agcaacaagt gccagacccc cctgggcatg gcctctggcc 4620
acatcaggga cttccagatc actgcctctg gccagtatgg ccagtgggcc cccaagctgg 4680
ccaggctgca ctactctggc agcatcaatg cctggagcac caaggagccc ttcagctgga 4740
tcaaggtgga cctgctggcc cccatgatca tccatggcat caagacccag ggggccaggc 4800
agaagttcag cagcctgtac atcagccagt tcatcatcat gtacagcctg gatggcaaga 4860
agtggcagac ctacaggggc aacagcactg gcaccctgat ggtgttcttt ggcaatgtgg 4920
acagctctgg catcaagcac aacatcttca acccccccat cattgccaga tacatcaggc 4980
tgcaccccac ccactacagc atcaggagca ccctgaggat ggagctgatg ggctgtgacc 5040
tgaacagctg cagcatgccc ctgggcatgg agagcaaggc catctctgat gcccagatca 5100
ctgccagcag ctacttcacc aacatgtttg ccacctggag ccccagcaag gccaggctgc 5160
acctgcaggg caggagcaat gcctggaggc cccaggtcaa caaccccaag gagtggctgc 5220
aggtggactt ccagaagacc atgaaggtga ctggggtgac cacccagggg gtgaagagcc 5280
tgctgaccag catgtatgtg aaggagttcc tgatcagcag cagccaggat ggccaccagt 5340
ggaccctgtt cttccagaat ggcaaggtga aggtgttcca gggcaaccag gacagcttca 5400
cccctgtggt gaacagcctg gacccccccc tgctgaccag atacctgagg attcaccccc 5460
agagctgggt gcaccagatt gccctgagga tggaggtgct gggctgtgag gcccaggacc 5520
tgtactgagc tcgagctgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 5580
tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 5640
ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 5700
gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 5760
accggtgcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg 5820
ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc 5880
ctcagtgagc gagcgagcgc gcagagaggg agtggccaa 5919
<210> 23
<211> 5306
<212> DNA
<213> 腺相关病毒2
<400> 23
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttaaacgt cgaccctaaa 180
atgggcaaac attgcaagca gcaaacagca aactgacctt ggagctgggg cagaggtcag 240
agacctctct gggcactcga ccccttggaa tttcggtgga gaggagcaga ggtacacagc 300
cctccctgcc tgccccatgc cacctccaac atctgtcctg gcgtggttta ggtagtgtga 360
gaggggaatg actcctttcg gtaagtgcag tggaagctgt acactgccca ggcaaagcgt 420
ccgggcagcg taggcgggcg actcagatcc cagccagtgg acttagcccc tgtttgctcc 480
tccgataact ggggtgacct tggttaatat tcaccagcag cctcccccgt tgcccctctg 540
gatccactgc ttaaatacgg acgaggacag ggccctgtct cctcagcttc aggcaccacc 600
actgacctgg gacagtgaat cgcgatcgca ctgcttaaat acggacgagg acagggccct 660
gtctcctcag cttcaggcac caccactgac ctgggacagt gaatcgcgat cgccaccatg 720
cagattgagc tgagcacctg cttcttcctg tgcctgctga ggttctgctt ctctgccacc 780
aggagatact acctgggggc tgtggagctg agctgggact acatgcagtc tgacctgggg 840
gagctgcctg tggatgccag gttccccccc agagtgccca agagcttccc cttcaacacc 900
tctgtggtgt acaagaagac cctgtttgtg gagttcactg accacctgtt caacattgcc 960
aagcccaggc ccccctggat gggcctgctg ggccccacca tccaggctga ggtgtatgac 1020
actgtggtga tcaccctgaa gaacatggcc agccaccctg tgagcctgca tgctgtgggg 1080
gtgagctact ggaaggcctc tgagggggct gagtatgatg accagaccag ccagagggag 1140
aaggaggatg acaaggtgtt ccctgggggc agccacacct atgtgtggca ggtgctgaag 1200
gagaatggcc ccatggcctc tgaccccctg tgcctgacct acagctacct gagccatgtg 1260
gacctggtga aggacctgaa ctctggcctg attggggccc tgctggtgtg cagggagggc 1320
agcctggcca aggagaagac ccagaccctg cacaagttca tcctgctgtt tgctgtgttt 1380
gatgagggca agagctggca ctctgaaacc aagaacagcc tgatgcagga cagggatgct 1440
gcctctgcca gggcctggcc caagatgcac actgtgaatg gctatgtgaa caggagcctg 1500
cctggcctga ttggctgcca caggaagtct gtgtactggc atgtgattgg catgggcacc 1560
acccctgagg tgcacagcat cttcctggag ggccacacct tcctggtcag gaaccacagg 1620
caggccagcc tggagatcag ccccatcacc ttcctgactg cccagaccct gctgatggac 1680
ctgggccagt tcctgctgtt ctgccacatc agcagccacc agcatgatgg catggaggcc 1740
tatgtgaagg tggacagctg ccctgaggag ccccagctga ggatgaagaa caatgaggag 1800
gctgaggact atgatgatga cctgactgac tctgagatgg atgtggtgag gtttgatgat 1860
gacaacagcc ccagcttcat ccagatcagg tctgtggcca agaagcaccc caagacctgg 1920
gtgcactaca ttgctgctga ggaggaggac tgggactatg cccccctggt gctggcccct 1980
gatgacagga gctacaagag ccagtacctg aacaatggcc cccagaggat tggcaggaag 2040
tacaagaagg tcaggttcat ggcctacact gatgaaacct tcaagaccag ggaggccatc 2100
cagcatgagt ctggcatcct gggccccctg ctgtatgggg aggtggggga caccctgctg 2160
atcatcttca agaaccaggc cagcaggccc tacaacatct acccccatgg catcactgat 2220
gtgaggcccc tgtacagcag gaggctgccc aagggggtga agcacctgaa ggacttcccc 2280
atcctgcctg gggagatctt caagtacaag tggactgtga ctgtggagga tggccccacc 2340
aagtctgacc ccaggtgcct gaccagatac tacagcagct ttgtgaacat ggagagggac 2400
ctggcctctg gcctgattgg ccccctgctg atctgctaca aggagtctgt ggaccagagg 2460
ggcaaccaga tcatgtctga caagaggaat gtgatcctgt tctctgtgtt tgatgagaac 2520
aggagctggt acctgactga gaacatccag aggttcctgc ccaaccctgc tggggtgcag 2580
ctggaggacc ctgagttcca ggccagcaac atcatgcaca gcatcaatgg ctatgtgttt 2640
gacagcctgc agctgtctgt gtgcctgcat gaggtggcct actggtacat cctgagcatt 2700
ggggcccaga ctgacttcct gtctgtgttc ttctctggct acaccttcaa gcacaagatg 2760
gtgtatgagg acaccctgac cctgttcccc ttctctgggg agactgtgtt catgagcatg 2820
gagaaccctg gcctgtggat tctgggctgc cacaactctg acttcaggaa caggggcatg 2880
actgccctgc tgaaagtctc cagctgtgac aagaacactg gggactacta tgaggacagc 2940
tatgaggaca tctctgccta cctgctgagc aagaacaatg ccattgagcc caggagcttc 3000
agccagaacc ccccagtgct gaagaggcac cagagggaga tcaccaggac caccctgcag 3060
tctgaccagg aggagattga ctatgatgac accatctctg tggagatgaa gaaggaggac 3120
tttgacatct acgacgagga cgagaaccag agccccagga gcttccagaa gaagaccagg 3180
cactacttca ttgctgctgt ggagaggctg tgggactatg gcatgagcag cagcccccat 3240
gtgctgagga acagggccca gtctggctct gtgccccagt tcaagaaggt ggtgttccag 3300
gagttcactg atggcagctt cacccagccc ctgtacagag gggagctgaa tgagcacctg 3360
ggcctgctgg gcccctacat cagggctgag gtggaggaca acatcatggt gaccttcagg 3420
aaccaggcca gcaggcccta cagcttctac agcagcctga tcagctatga ggaggaccag 3480
aggcaggggg ctgagcccag gaagaacttt gtgaagccca atgaaaccaa gacctacttc 3540
tggaaggtgc agcaccacat ggcccccacc aaggatgagt ttgactgcaa ggcctgggcc 3600
tacttctctg atgtggacct ggagaaggat gtgcactctg gcctgattgg ccccctgctg 3660
gtgtgccaca ccaacaccct gaaccctgcc catggcaggc aggtgactgt gcaggagttt 3720
gccctgttct tcaccatctt tgatgaaacc aagagctggt acttcactga gaacatggag 3780
aggaactgca gggccccctg caacatccag atggaggacc ccaccttcaa ggagaactac 3840
aggttccatg ccatcaatgg ctacatcatg gacaccctgc ctggcctggt gatggcccag 3900
gaccagagga tcaggtggta cctgctgagc atgggcagca atgagaacat ccacagcatc 3960
cacttctctg gccatgtgtt cactgtgagg aagaaggagg agtacaagat ggccctgtac 4020
aacctgtacc ctggggtgtt tgagactgtg gagatgctgc ccagcaaggc tggcatctgg 4080
agggtggagt gcctgattgg ggagcacctg catgctggca tgagcaccct gttcctggtg 4140
tacagcaaca agtgccagac ccccctgggc atggcctctg gccacatcag ggacttccag 4200
atcactgcct ctggccagta tggccagtgg gcccccaagc tggccaggct gcactactct 4260
ggcagcatca atgcctggag caccaaggag cccttcagct ggatcaaggt ggacctgctg 4320
gcccccatga tcatccatgg catcaagacc cagggggcca ggcagaagtt cagcagcctg 4380
tacatcagcc agttcatcat catgtacagc ctggatggca agaagtggca gacctacagg 4440
ggcaacagca ctggcaccct gatggtgttc tttggcaatg tggacagctc tggcatcaag 4500
cacaacatct tcaacccccc catcattgcc agatacatca ggctgcaccc cacccactac 4560
agcatcagga gcaccctgag gatggagctg atgggctgtg acctgaacag ctgcagcatg 4620
cccctgggca tggagagcaa ggccatctct gatgcccaga tcactgccag cagctacttc 4680
accaacatgt ttgccacctg gagccccagc aaggccaggc tgcacctgca gggcaggagc 4740
aatgcctgga ggccccaggt caacaacccc aaggagtggc tgcaggtgga cttccagaag 4800
accatgaagg tgactggggt gaccacccag ggggtgaaga gcctgctgac cagcatgtat 4860
gtgaaggagt tcctgatcag cagcagccag gatggccacc agtggaccct gttcttccag 4920
aatggcaagg tgaaggtgtt ccagggcaac caggacagct tcacccctgt ggtgaacagc 4980
ctggaccccc ccctgctgac cagatacctg aggattcacc cccagagctg ggtgcaccag 5040
attgccctga ggatggaggt gctgggctgt gaggcccagg acctgtactg acctcgagga 5100
ataaaggaaa tttattttca ttgcaatagt gtgttggttt tttgtgtcac gtggcggccg 5160
caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag 5220
gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag 5280
cgagcgcgca gagagggagt ggccaa 5306
<210> 24
<211> 5461
<212> DNA
<213> 腺相关病毒2
<400> 24
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactt tatttgccac 180
aaaaacccta tcagatgggc gtctttatca tttccattgt acagatgggg aaacaggctt 240
cggggtcggg gcatagccac ttactgacga ctccccaccc agcaagtggt tttgaacccg 300
gaccctctca cactacctaa accacgccag gacaacctct gctcctctcc accgaaattc 360
caaggggtcg agtggatgtt ggaggtggca tgggcccaga gaggtctctg acctctgccc 420
cagctccaag gtcagcaggc agggagggct gtgtgtttgc tgtttgctgc ttgcaatgtt 480
tgcccatttt agggacatga gtaggctgaa gtttgttcag tgtggacttc agaggcagca 540
cacaaacagc tgctggagga tgggaactga ggggttggaa gggggcaggg tgagcccaga 600
aactcctgtg tgcctctgag cctgcagacg cgaaacgtcg actggacaca ggacgctgtg 660
gtttctgagc cagggggcga ctcagatccc agccagtgga cttagcccct gtttgctcct 720
ccgataactg gggtgacctt ggttaatatt caccagcagc ctcccccgtt gcccctctgg 780
atccactgct taaatacgga cgaggacagg gccctgtctc ctcagcttca ggcaccacca 840
ctgacctggg acagtgaatc gcgatcgcca ccatgcagat tgagctgagc acctgcttct 900
tcctgtgcct gctgaggttc tgcttctctg ccaccaggag atactacctg ggggctgtgg 960
agctgagctg ggactacatg cagtctgacc tgggggagct gcctgtggat gccaggttcc 1020
cccccagagt gcccaagagc ttccccttca acacctctgt ggtgtacaag aagaccctgt 1080
ttgtggagtt cactgaccac ctgttcaaca ttgccaagcc caggcccccc tggatgggcc 1140
tgctgggccc caccatccag gctgaggtgt atgacactgt ggtgatcacc ctgaagaaca 1200
tggccagcca ccctgtgagc ctgcatgctg tgggggtgag ctactggaag gcctctgagg 1260
gggctgagta tgatgaccag accagccaga gggagaagga ggatgacaag gtgttccctg 1320
ggggcagcca cacctatgtg tggcaggtgc tgaaggagaa tggccccatg gcctctgacc 1380
ccctgtgcct gacctacagc tacctgagcc atgtggacct ggtgaaggac ctgaactctg 1440
gcctgattgg ggccctgctg gtgtgcaggg agggcagcct ggccaaggag aagacccaga 1500
ccctgcacaa gttcatcctg ctgtttgctg tgtttgatga gggcaagagc tggcactctg 1560
aaaccaagaa cagcctgatg caggacaggg atgctgcctc tgccagggcc tggcccaaga 1620
tgcacactgt gaatggctat gtgaacagga gcctgcctgg cctgattggc tgccacagga 1680
agtctgtgta ctggcatgtg attggcatgg gcaccacccc tgaggtgcac agcatcttcc 1740
tggagggcca caccttcctg gtcaggaacc acaggcaggc cagcctggag atcagcccca 1800
tcaccttcct gactgcccag accctgctga tggacctggg ccagttcctg ctgttctgcc 1860
acatcagcag ccaccagcat gatggcatgg aggcctatgt gaaggtggac agctgccctg 1920
aggagcccca gctgaggatg aagaacaatg aggaggctga ggactatgat gatgacctga 1980
ctgactctga gatggatgtg gtgaggtttg atgatgacaa cagccccagc ttcatccaga 2040
tcaggtctgt ggccaagaag caccccaaga cctgggtgca ctacattgct gctgaggagg 2100
aggactggga ctatgccccc ctggtgctgg cccctgatga caggagctac aagagccagt 2160
acctgaacaa tggcccccag aggattggca ggaagtacaa gaaggtcagg ttcatggcct 2220
acactgatga aaccttcaag accagggagg ccatccagca tgagtctggc atcctgggcc 2280
ccctgctgta tggggaggtg ggggacaccc tgctgatcat cttcaagaac caggccagca 2340
ggccctacaa catctacccc catggcatca ctgatgtgag gcccctgtac agcaggaggc 2400
tgcccaaggg ggtgaagcac ctgaaggact tccccatcct gcctggggag atcttcaagt 2460
acaagtggac tgtgactgtg gaggatggcc ccaccaagtc tgaccccagg tgcctgacca 2520
gatactacag cagctttgtg aacatggaga gggacctggc ctctggcctg attggccccc 2580
tgctgatctg ctacaaggag tctgtggacc agaggggcaa ccagatcatg tctgacaaga 2640
ggaatgtgat cctgttctct gtgtttgatg agaacaggag ctggtacctg actgagaaca 2700
tccagaggtt cctgcccaac cctgctgggg tgcagctgga ggaccctgag ttccaggcca 2760
gcaacatcat gcacagcatc aatggctatg tgtttgacag cctgcagctg tctgtgtgcc 2820
tgcatgaggt ggcctactgg tacatcctga gcattggggc ccagactgac ttcctgtctg 2880
tgttcttctc tggctacacc ttcaagcaca agatggtgta tgaggacacc ctgaccctgt 2940
tccccttctc tggggagact gtgttcatga gcatggagaa ccctggcctg tggattctgg 3000
gctgccacaa ctctgacttc aggaacaggg gcatgactgc cctgctgaaa gtctccagct 3060
gtgacaagaa cactggggac tactatgagg acagctatga ggacatctct gcctacctgc 3120
tgagcaagaa caatgccatt gagcccagga gcttcagcca gaacccccca gtgctgaaga 3180
ggcaccagag ggagatcacc aggaccaccc tgcagtctga ccaggaggag attgactatg 3240
atgacaccat ctctgtggag atgaagaagg aggactttga catctacgac gaggacgaga 3300
accagagccc caggagcttc cagaagaaga ccaggcacta cttcattgct gctgtggaga 3360
ggctgtggga ctatggcatg agcagcagcc cccatgtgct gaggaacagg gcccagtctg 3420
gctctgtgcc ccagttcaag aaggtggtgt tccaggagtt cactgatggc agcttcaccc 3480
agcccctgta cagaggggag ctgaatgagc acctgggcct gctgggcccc tacatcaggg 3540
ctgaggtgga ggacaacatc atggtgacct tcaggaacca ggccagcagg ccctacagct 3600
tctacagcag cctgatcagc tatgaggagg accagaggca gggggctgag cccaggaaga 3660
actttgtgaa gcccaatgaa accaagacct acttctggaa ggtgcagcac cacatggccc 3720
ccaccaagga tgagtttgac tgcaaggcct gggcctactt ctctgatgtg gacctggaga 3780
aggatgtgca ctctggcctg attggccccc tgctggtgtg ccacaccaac accctgaacc 3840
ctgcccatgg caggcaggtg actgtgcagg agtttgccct gttcttcacc atctttgatg 3900
aaaccaagag ctggtacttc actgagaaca tggagaggaa ctgcagggcc ccctgcaaca 3960
tccagatgga ggaccccacc ttcaaggaga actacaggtt ccatgccatc aatggctaca 4020
tcatggacac cctgcctggc ctggtgatgg cccaggacca gaggatcagg tggtacctgc 4080
tgagcatggg cagcaatgag aacatccaca gcatccactt ctctggccat gtgttcactg 4140
tgaggaagaa ggaggagtac aagatggccc tgtacaacct gtaccctggg gtgtttgaga 4200
ctgtggagat gctgcccagc aaggctggca tctggagggt ggagtgcctg attggggagc 4260
acctgcatgc tggcatgagc accctgttcc tggtgtacag caacaagtgc cagacccccc 4320
tgggcatggc ctctggccac atcagggact tccagatcac tgcctctggc cagtatggcc 4380
agtgggcccc caagctggcc aggctgcact actctggcag catcaatgcc tggagcacca 4440
aggagccctt cagctggatc aaggtggacc tgctggcccc catgatcatc catggcatca 4500
agacccaggg ggccaggcag aagttcagca gcctgtacat cagccagttc atcatcatgt 4560
acagcctgga tggcaagaag tggcagacct acaggggcaa cagcactggc accctgatgg 4620
tgttctttgg caatgtggac agctctggca tcaagcacaa catcttcaac ccccccatca 4680
ttgccagata catcaggctg caccccaccc actacagcat caggagcacc ctgaggatgg 4740
agctgatggg ctgtgacctg aacagctgca gcatgcccct gggcatggag agcaaggcca 4800
tctctgatgc ccagatcact gccagcagct acttcaccaa catgtttgcc acctggagcc 4860
ccagcaaggc caggctgcac ctgcagggca ggagcaatgc ctggaggccc caggtcaaca 4920
accccaagga gtggctgcag gtggacttcc agaagaccat gaaggtgact ggggtgacca 4980
cccagggggt gaagagcctg ctgaccagca tgtatgtgaa ggagttcctg atcagcagca 5040
gccaggatgg ccaccagtgg accctgttct tccagaatgg caaggtgaag gtgttccagg 5100
gcaaccagga cagcttcacc cctgtggtga acagcctgga cccccccctg ctgaccagat 5160
acctgaggat tcacccccag agctgggtgc accagattgc cctgaggatg gaggtgctgg 5220
gctgtgaggc ccaggacctg tactgacctc gaggaataaa ggaaatttat tttcattgca 5280
atagtgtgtt ggttttttgt gtcacgtggc ggccgcagga acccctagtg atggagttgg 5340
ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac 5400
gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 5460
a 5461
<210> 25
<211> 5327
<212> DNA
<213> 腺相关病毒2
<400> 25
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct gtgcctgctg 780
aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct gagctgggac 840
tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc cagagtgccc 900
aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt ggagttcact 960
gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct gggccccacc 1020
atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc cagccaccct 1080
gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc tgagtatgat 1140
gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg cagccacacc 1200
tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct gtgcctgacc 1260
tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct gattggggcc 1320
ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct gcacaagttc 1380
atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac caagaacagc 1440
ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca cactgtgaat 1500
ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc tgtgtactgg 1560
catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga gggccacacc 1620
ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac cttcctgact 1680
gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat cagcagccac 1740
cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga gccccagctg 1800
aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga ctctgagatg 1860
gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag gtctgtggcc 1920
aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga ctgggactat 1980
gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct gaacaatggc 2040
ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac tgatgaaacc 2100
ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct gctgtatggg 2160
gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc ctacaacatc 2220
tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc caagggggtg 2280
aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa gtggactgtg 2340
actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata ctacagcagc 2400
tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct gatctgctac 2460
aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa tgtgatcctg 2520
ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca gaggttcctg 2580
cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa catcatgcac 2640
agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc 2700
tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt cttctctggc 2760
tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc cttctctggg 2820
gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg ccacaactct 2880
gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga caagaacact 2940
ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag caagaacaat 3000
gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca ccagagggag 3060
atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga caccatctct 3120
gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca gagccccagg 3180
agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct gtgggactat 3240
ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc tgtgccccag 3300
ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc cctgtacaga 3360
ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga ggtggaggac 3420
aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta cagcagcctg 3480
atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt tgtgaagccc 3540
aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac caaggatgag 3600
tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga tgtgcactct 3660
ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc ccatggcagg 3720
caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac caagagctgg 3780
tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca gatggaggac 3840
cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat ggacaccctg 3900
cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag catgggcagc 3960
aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag gaagaaggag 4020
gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt ggagatgctg 4080
cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct gcatgctggc 4140
atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg catggcctct 4200
ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg ggcccccaag 4260
ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga gcccttcagc 4320
tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac ccagggggcc 4380
aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag cctggatggc 4440
aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt ctttggcaat 4500
gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc cagatacatc 4560
aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct gatgggctgt 4620
gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc tgatgcccag 4680
atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag caaggccagg 4740
ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc caaggagtgg 4800
ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca gggggtgaag 4860
agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca ggatggccac 4920
cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa ccaggacagc 4980
ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct gaggattcac 5040
ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg tgaggcccag 5100
gacctgtact gacctcgagg aataaaggaa atttattttc attgcaatag tgtgttggtt 5160
ttttgtgtca cgtggcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 5220
cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 5280
cgggcggcct cagtgagcga gcgagcgcgc agagagggag tggccaa 5327
<210> 26
<211> 5309
<212> DNA
<213> 腺相关病毒2
<400> 26
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtc tgcaggctca gaggcacaca 180
ggagtttctg ggctcaccct gcccccttcc aacccctcag ttcccatcct ccagcagctg 240
tttgtgtgct gcctctgaag tccacactga acaaacttca gcctactcat gtccctaaaa 300
tgggcaaaca ttgcaagcag caaacagcaa acacacagcc ctccctgcct gctgaccttg 360
gagctggggc agaggtcaga gacctctctg ggcccatgcc acctccaaca tccactcgac 420
cccttggaat ttcggtggag aggagcagag gttgtcctgg cgtggtttag gtagtgtgag 480
aggggtcgac tggacacagg acgctgtggt ttctgagcca gggggcgact cagatcccag 540
ccagtggact tagcccctgt ttgctcctcc gataactggg gtgaccttgg ttaatattca 600
ccagcagcct cccccgttgc ccctctggat ccactgctta aatacggacg aggacagggc 660
cctgtctcct cagcttcagg caccaccact gacctgggac agtgaatcgc gatcgccacc 720
atgcagattg agctgagcac ctgcttcttc ctgtgcctgc tgaggttctg cttctctgcc 780
accaggagat actacctggg ggctgtggag ctgagctggg actacatgca gtctgacctg 840
ggggagctgc ctgtggatgc caggttcccc cccagagtgc ccaagagctt ccccttcaac 900
acctctgtgg tgtacaagaa gaccctgttt gtggagttca ctgaccacct gttcaacatt 960
gccaagccca ggcccccctg gatgggcctg ctgggcccca ccatccaggc tgaggtgtat 1020
gacactgtgg tgatcaccct gaagaacatg gccagccacc ctgtgagcct gcatgctgtg 1080
ggggtgagct actggaaggc ctctgagggg gctgagtatg atgaccagac cagccagagg 1140
gagaaggagg atgacaaggt gttccctggg ggcagccaca cctatgtgtg gcaggtgctg 1200
aaggagaatg gccccatggc ctctgacccc ctgtgcctga cctacagcta cctgagccat 1260
gtggacctgg tgaaggacct gaactctggc ctgattgggg ccctgctggt gtgcagggag 1320
ggcagcctgg ccaaggagaa gacccagacc ctgcacaagt tcatcctgct gtttgctgtg 1380
tttgatgagg gcaagagctg gcactctgaa accaagaaca gcctgatgca ggacagggat 1440
gctgcctctg ccagggcctg gcccaagatg cacactgtga atggctatgt gaacaggagc 1500
ctgcctggcc tgattggctg ccacaggaag tctgtgtact ggcatgtgat tggcatgggc 1560
accacccctg aggtgcacag catcttcctg gagggccaca ccttcctggt caggaaccac 1620
aggcaggcca gcctggagat cagccccatc accttcctga ctgcccagac cctgctgatg 1680
gacctgggcc agttcctgct gttctgccac atcagcagcc accagcatga tggcatggag 1740
gcctatgtga aggtggacag ctgccctgag gagccccagc tgaggatgaa gaacaatgag 1800
gaggctgagg actatgatga tgacctgact gactctgaga tggatgtggt gaggtttgat 1860
gatgacaaca gccccagctt catccagatc aggtctgtgg ccaagaagca ccccaagacc 1920
tgggtgcact acattgctgc tgaggaggag gactgggact atgcccccct ggtgctggcc 1980
cctgatgaca ggagctacaa gagccagtac ctgaacaatg gcccccagag gattggcagg 2040
aagtacaaga aggtcaggtt catggcctac actgatgaaa ccttcaagac cagggaggcc 2100
atccagcatg agtctggcat cctgggcccc ctgctgtatg gggaggtggg ggacaccctg 2160
ctgatcatct tcaagaacca ggccagcagg ccctacaaca tctaccccca tggcatcact 2220
gatgtgaggc ccctgtacag caggaggctg cccaaggggg tgaagcacct gaaggacttc 2280
cccatcctgc ctggggagat cttcaagtac aagtggactg tgactgtgga ggatggcccc 2340
accaagtctg accccaggtg cctgaccaga tactacagca gctttgtgaa catggagagg 2400
gacctggcct ctggcctgat tggccccctg ctgatctgct acaaggagtc tgtggaccag 2460
aggggcaacc agatcatgtc tgacaagagg aatgtgatcc tgttctctgt gtttgatgag 2520
aacaggagct ggtacctgac tgagaacatc cagaggttcc tgcccaaccc tgctggggtg 2580
cagctggagg accctgagtt ccaggccagc aacatcatgc acagcatcaa tggctatgtg 2640
tttgacagcc tgcagctgtc tgtgtgcctg catgaggtgg cctactggta catcctgagc 2700
attggggccc agactgactt cctgtctgtg ttcttctctg gctacacctt caagcacaag 2760
atggtgtatg aggacaccct gaccctgttc cccttctctg gggagactgt gttcatgagc 2820
atggagaacc ctggcctgtg gattctgggc tgccacaact ctgacttcag gaacaggggc 2880
atgactgccc tgctgaaagt ctccagctgt gacaagaaca ctggggacta ctatgaggac 2940
agctatgagg acatctctgc ctacctgctg agcaagaaca atgccattga gcccaggagc 3000
ttcagccaga accccccagt gctgaagagg caccagaggg agatcaccag gaccaccctg 3060
cagtctgacc aggaggagat tgactatgat gacaccatct ctgtggagat gaagaaggag 3120
gactttgaca tctacgacga ggacgagaac cagagcccca ggagcttcca gaagaagacc 3180
aggcactact tcattgctgc tgtggagagg ctgtgggact atggcatgag cagcagcccc 3240
catgtgctga ggaacagggc ccagtctggc tctgtgcccc agttcaagaa ggtggtgttc 3300
caggagttca ctgatggcag cttcacccag cccctgtaca gaggggagct gaatgagcac 3360
ctgggcctgc tgggccccta catcagggct gaggtggagg acaacatcat ggtgaccttc 3420
aggaaccagg ccagcaggcc ctacagcttc tacagcagcc tgatcagcta tgaggaggac 3480
cagaggcagg gggctgagcc caggaagaac tttgtgaagc ccaatgaaac caagacctac 3540
ttctggaagg tgcagcacca catggccccc accaaggatg agtttgactg caaggcctgg 3600
gcctacttct ctgatgtgga cctggagaag gatgtgcact ctggcctgat tggccccctg 3660
ctggtgtgcc acaccaacac cctgaaccct gcccatggca ggcaggtgac tgtgcaggag 3720
tttgccctgt tcttcaccat ctttgatgaa accaagagct ggtacttcac tgagaacatg 3780
gagaggaact gcagggcccc ctgcaacatc cagatggagg accccacctt caaggagaac 3840
tacaggttcc atgccatcaa tggctacatc atggacaccc tgcctggcct ggtgatggcc 3900
caggaccaga ggatcaggtg gtacctgctg agcatgggca gcaatgagaa catccacagc 3960
atccacttct ctggccatgt gttcactgtg aggaagaagg aggagtacaa gatggccctg 4020
tacaacctgt accctggggt gtttgagact gtggagatgc tgcccagcaa ggctggcatc 4080
tggagggtgg agtgcctgat tggggagcac ctgcatgctg gcatgagcac cctgttcctg 4140
gtgtacagca acaagtgcca gacccccctg ggcatggcct ctggccacat cagggacttc 4200
cagatcactg cctctggcca gtatggccag tgggccccca agctggccag gctgcactac 4260
tctggcagca tcaatgcctg gagcaccaag gagcccttca gctggatcaa ggtggacctg 4320
ctggccccca tgatcatcca tggcatcaag acccaggggg ccaggcagaa gttcagcagc 4380
ctgtacatca gccagttcat catcatgtac agcctggatg gcaagaagtg gcagacctac 4440
aggggcaaca gcactggcac cctgatggtg ttctttggca atgtggacag ctctggcatc 4500
aagcacaaca tcttcaaccc ccccatcatt gccagataca tcaggctgca ccccacccac 4560
tacagcatca ggagcaccct gaggatggag ctgatgggct gtgacctgaa cagctgcagc 4620
atgcccctgg gcatggagag caaggccatc tctgatgccc agatcactgc cagcagctac 4680
ttcaccaaca tgtttgccac ctggagcccc agcaaggcca ggctgcacct gcagggcagg 4740
agcaatgcct ggaggcccca ggtcaacaac cccaaggagt ggctgcaggt ggacttccag 4800
aagaccatga aggtgactgg ggtgaccacc cagggggtga agagcctgct gaccagcatg 4860
tatgtgaagg agttcctgat cagcagcagc caggatggcc accagtggac cctgttcttc 4920
cagaatggca aggtgaaggt gttccagggc aaccaggaca gcttcacccc tgtggtgaac 4980
agcctggacc cccccctgct gaccagatac ctgaggattc acccccagag ctgggtgcac 5040
cagattgccc tgaggatgga ggtgctgggc tgtgaggccc aggacctgta ctgacctcga 5100
ggaataaagg aaatttattt tcattgcaat agtgtgttgg ttttttgtgt cacgtggcgg 5160
ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact 5220
gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc 5280
gagcgagcgc gcagagaggg agtggccaa 5309
<210> 27
<211> 5532
<212> DNA
<213> 腺相关病毒2
<400> 27
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgtaa gtatgccttt cactgcgaga ggttctggag aggcttctga gctccccatg 780
gcccaggcag gcagcaggtc tggggcagga ggggggttgt ggagtgcctt gactcggggc 840
ctggcccccc catctctgtc ttgcaggaca attgccgtct tctgtctcgt ggggcatcct 900
cctgctggca ggcctgtgct gcctggtccc tgcgatcgcc accatgcaga ttgagctgag 960
cacctgcttc ttcctgtgcc tgctgaggtt ctgcttctct gccaccagga gatactacct 1020
gggggctgtg gagctgagct gggactacat gcagtctgac ctgggggagc tgcctgtgga 1080
tgccaggttc ccccccagag tgcccaagag cttccccttc aacacctctg tggtgtacaa 1140
gaagaccctg tttgtggagt tcactgacca cctgttcaac attgccaagc ccaggccccc 1200
ctggatgggc ctgctgggcc ccaccatcca ggctgaggtg tatgacactg tggtgatcac 1260
cctgaagaac atggccagcc accctgtgag cctgcatgct gtgggggtga gctactggaa 1320
ggcctctgag ggggctgagt atgatgacca gaccagccag agggagaagg aggatgacaa 1380
ggtgttccct gggggcagcc acacctatgt gtggcaggtg ctgaaggaga atggccccat 1440
ggcctctgac cccctgtgcc tgacctacag ctacctgagc catgtggacc tggtgaagga 1500
cctgaactct ggcctgattg gggccctgct ggtgtgcagg gagggcagcc tggccaagga 1560
gaagacccag accctgcaca agttcatcct gctgtttgct gtgtttgatg agggcaagag 1620
ctggcactct gaaaccaaga acagcctgat gcaggacagg gatgctgcct ctgccagggc 1680
ctggcccaag atgcacactg tgaatggcta tgtgaacagg agcctgcctg gcctgattgg 1740
ctgccacagg aagtctgtgt actggcatgt gattggcatg ggcaccaccc ctgaggtgca 1800
cagcatcttc ctggagggcc acaccttcct ggtcaggaac cacaggcagg ccagcctgga 1860
gatcagcccc atcaccttcc tgactgccca gaccctgctg atggacctgg gccagttcct 1920
gctgttctgc cacatcagca gccaccagca tgatggcatg gaggcctatg tgaaggtgga 1980
cagctgccct gaggagcccc agctgaggat gaagaacaat gaggaggctg aggactatga 2040
tgatgacctg actgactctg agatggatgt ggtgaggttt gatgatgaca acagccccag 2100
cttcatccag atcaggtctg tggccaagaa gcaccccaag acctgggtgc actacattgc 2160
tgctgaggag gaggactggg actatgcccc cctggtgctg gcccctgatg acaggagcta 2220
caagagccag tacctgaaca atggccccca gaggattggc aggaagtaca agaaggtcag 2280
gttcatggcc tacactgatg aaaccttcaa gaccagggag gccatccagc atgagtctgg 2340
catcctgggc cccctgctgt atggggaggt gggggacacc ctgctgatca tcttcaagaa 2400
ccaggccagc aggccctaca acatctaccc ccatggcatc actgatgtga ggcccctgta 2460
cagcaggagg ctgcccaagg gggtgaagca cctgaaggac ttccccatcc tgcctgggga 2520
gatcttcaag tacaagtgga ctgtgactgt ggaggatggc cccaccaagt ctgaccccag 2580
gtgcctgacc agatactaca gcagctttgt gaacatggag agggacctgg cctctggcct 2640
gattggcccc ctgctgatct gctacaagga gtctgtggac cagaggggca accagatcat 2700
gtctgacaag aggaatgtga tcctgttctc tgtgtttgat gagaacagga gctggtacct 2760
gactgagaac atccagaggt tcctgcccaa ccctgctggg gtgcagctgg aggaccctga 2820
gttccaggcc agcaacatca tgcacagcat caatggctat gtgtttgaca gcctgcagct 2880
gtctgtgtgc ctgcatgagg tggcctactg gtacatcctg agcattgggg cccagactga 2940
cttcctgtct gtgttcttct ctggctacac cttcaagcac aagatggtgt atgaggacac 3000
cctgaccctg ttccccttct ctggggagac tgtgttcatg agcatggaga accctggcct 3060
gtggattctg ggctgccaca actctgactt caggaacagg ggcatgactg ccctgctgaa 3120
agtctccagc tgtgacaaga acactgggga ctactatgag gacagctatg aggacatctc 3180
tgcctacctg ctgagcaaga acaatgccat tgagcccagg agcttcagcc agaacccccc 3240
agtgctgaag aggcaccaga gggagatcac caggaccacc ctgcagtctg accaggagga 3300
gattgactat gatgacacca tctctgtgga gatgaagaag gaggactttg acatctacga 3360
cgaggacgag aaccagagcc ccaggagctt ccagaagaag accaggcact acttcattgc 3420
tgctgtggag aggctgtggg actatggcat gagcagcagc ccccatgtgc tgaggaacag 3480
ggcccagtct ggctctgtgc cccagttcaa gaaggtggtg ttccaggagt tcactgatgg 3540
cagcttcacc cagcccctgt acagagggga gctgaatgag cacctgggcc tgctgggccc 3600
ctacatcagg gctgaggtgg aggacaacat catggtgacc ttcaggaacc aggccagcag 3660
gccctacagc ttctacagca gcctgatcag ctatgaggag gaccagaggc agggggctga 3720
gcccaggaag aactttgtga agcccaatga aaccaagacc tacttctgga aggtgcagca 3780
ccacatggcc cccaccaagg atgagtttga ctgcaaggcc tgggcctact tctctgatgt 3840
ggacctggag aaggatgtgc actctggcct gattggcccc ctgctggtgt gccacaccaa 3900
caccctgaac cctgcccatg gcaggcaggt gactgtgcag gagtttgccc tgttcttcac 3960
catctttgat gaaaccaaga gctggtactt cactgagaac atggagagga actgcagggc 4020
cccctgcaac atccagatgg aggaccccac cttcaaggag aactacaggt tccatgccat 4080
caatggctac atcatggaca ccctgcctgg cctggtgatg gcccaggacc agaggatcag 4140
gtggtacctg ctgagcatgg gcagcaatga gaacatccac agcatccact tctctggcca 4200
tgtgttcact gtgaggaaga aggaggagta caagatggcc ctgtacaacc tgtaccctgg 4260
ggtgtttgag actgtggaga tgctgcccag caaggctggc atctggaggg tggagtgcct 4320
gattggggag cacctgcatg ctggcatgag caccctgttc ctggtgtaca gcaacaagtg 4380
ccagaccccc ctgggcatgg cctctggcca catcagggac ttccagatca ctgcctctgg 4440
ccagtatggc cagtgggccc ccaagctggc caggctgcac tactctggca gcatcaatgc 4500
ctggagcacc aaggagccct tcagctggat caaggtggac ctgctggccc ccatgatcat 4560
ccatggcatc aagacccagg gggccaggca gaagttcagc agcctgtaca tcagccagtt 4620
catcatcatg tacagcctgg atggcaagaa gtggcagacc tacaggggca acagcactgg 4680
caccctgatg gtgttctttg gcaatgtgga cagctctggc atcaagcaca acatcttcaa 4740
cccccccatc attgccagat acatcaggct gcaccccacc cactacagca tcaggagcac 4800
cctgaggatg gagctgatgg gctgtgacct gaacagctgc agcatgcccc tgggcatgga 4860
gagcaaggcc atctctgatg cccagatcac tgccagcagc tacttcacca acatgtttgc 4920
cacctggagc cccagcaagg ccaggctgca cctgcagggc aggagcaatg cctggaggcc 4980
ccaggtcaac aaccccaagg agtggctgca ggtggacttc cagaagacca tgaaggtgac 5040
tggggtgacc acccaggggg tgaagagcct gctgaccagc atgtatgtga aggagttcct 5100
gatcagcagc agccaggatg gccaccagtg gaccctgttc ttccagaatg gcaaggtgaa 5160
ggtgttccag ggcaaccagg acagcttcac ccctgtggtg aacagcctgg acccccccct 5220
gctgaccaga tacctgagga ttcaccccca gagctgggtg caccagattg ccctgaggat 5280
ggaggtgctg ggctgtgagg cccaggacct gtactgacct cgaggaataa aggaaattta 5340
ttttcattgc aatagtgtgt tggttttttg tgtcacgtgg cggccgcagg aacccctagt 5400
gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa 5460
ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagaga 5520
gggagtggcc aa 5532
<210> 28
<211> 5877
<212> DNA
<213> 腺相关病毒2
<400> 28
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgacag gttaattttt aaaaagcagt caaaagtcca 540
agtggccctt ggcagcattt actctctctg tttgctctgg ttaataatct caggagcaca 600
aacattcctg gaggcaggag aagaaatcaa catcctggac ttatcctctg ggcctctccc 660
cacccccagg agaggctcag gttaattttt aaaaagcagt caaaagtcca agtggccctt 720
ggcagcattt actctctctg tttgctctgg ttaataatct caggagcaca aacattcctg 780
gaggcaggag aagaaatcaa catcctggac ttatcctctg ggcctctccc cacccccagg 840
agaggctgtc gactggacac aggacgctgt ggtttctgag ccagggggcg actcagatcc 900
cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct tggttaatat 960
tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg acgaggacag 1020
ggccctgtct cctcagcttc aggcaccacc actgacctgg gacagtgaat cgtaagtatg 1080
cctttcactg cgagaggttc tggagaggct tctgagctcc ccatggccca ggcaggcagc 1140
aggtctgggg caggaggggg gttgtggagt gccttgactc ggggcctggc ccccccatct 1200
ctgtcttgca ggacaattgc cgtcttctgt ctcgtggggc atcctcctgc tggcaggcct 1260
gtgctgcctg gtccctgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct 1320
gtgcctgctg aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct 1380
gagctgggac tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc 1440
cagagtgccc aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt 1500
ggagttcact gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct 1560
gggccccacc atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc 1620
cagccaccct gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc 1680
tgagtatgat gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg 1740
cagccacacc tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct 1800
gtgcctgacc tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct 1860
gattggggcc ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct 1920
gcacaagttc atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac 1980
caagaacagc ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca 2040
cactgtgaat ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc 2100
tgtgtactgg catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga 2160
gggccacacc ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac 2220
cttcctgact gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat 2280
cagcagccac cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga 2340
gccccagctg aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga 2400
ctctgagatg gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag 2460
gtctgtggcc aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga 2520
ctgggactat gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct 2580
gaacaatggc ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac 2640
tgatgaaacc ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct 2700
gctgtatggg gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc 2760
ctacaacatc tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc 2820
caagggggtg aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa 2880
gtggactgtg actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata 2940
ctacagcagc tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct 3000
gatctgctac aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa 3060
tgtgatcctg ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca 3120
gaggttcctg cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa 3180
catcatgcac agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca 3240
tgaggtggcc tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt 3300
cttctctggc tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc 3360
cttctctggg gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg 3420
ccacaactct gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga 3480
caagaacact ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag 3540
caagaacaat gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca 3600
ccagagggag atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga 3660
caccatctct gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca 3720
gagccccagg agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct 3780
gtgggactat ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc 3840
tgtgccccag ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc 3900
cctgtacaga ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga 3960
ggtggaggac aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta 4020
cagcagcctg atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt 4080
tgtgaagccc aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac 4140
caaggatgag tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga 4200
tgtgcactct ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc 4260
ccatggcagg caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac 4320
caagagctgg tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca 4380
gatggaggac cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat 4440
ggacaccctg cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag 4500
catgggcagc aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag 4560
gaagaaggag gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt 4620
ggagatgctg cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct 4680
gcatgctggc atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg 4740
catggcctct ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg 4800
ggcccccaag ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga 4860
gcccttcagc tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac 4920
ccagggggcc aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag 4980
cctggatggc aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt 5040
ctttggcaat gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc 5100
cagatacatc aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct 5160
gatgggctgt gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc 5220
tgatgcccag atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag 5280
caaggccagg ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc 5340
caaggagtgg ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca 5400
gggggtgaag agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca 5460
ggatggccac cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa 5520
ccaggacagc ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct 5580
gaggattcac ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg 5640
tgaggcccag gacctgtact gacctcgagg aataaaggaa atttattttc attgcaatag 5700
tgtgttggtt ttttgtgtca cgtggcggcc gcaggaaccc ctagtgatgg agttggccac 5760
tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 5820
gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag tggccaa 5877
<210> 29
<211> 6054
<212> DNA
<213> 腺相关病毒2
<400> 29
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgacag gttaattttt aaaaagcagt caaaagtcca 540
agtggccctt ggcagcattt actctctctg tttgctctgg ttaataatct caggagcaca 600
aacattcctg gaggcaggag aagaaatcaa catcctggac ttatcctctg ggcctctccc 660
cacccccagg agaggctcag gttaattttt aaaaagcagt caaaagtcca agtggccctt 720
ggcagcattt actctctctg tttgctctgg ttaataatct caggagcaca aacattcctg 780
gaggcaggag aagaaatcaa catcctggac ttatcctctg ggcctctccc cacccccagg 840
agaggctgtc gactggacac aggacgctgt ggtttctgag ccagggggcg actcagatcc 900
cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct tggttaatat 960
tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg acgaggacag 1020
ggccctgtct cctcagcttc aggcaccacc actgacctgg gacagtgaat cgtaagtatg 1080
cctttcactg cgagaggttc tggagaggct tctgagctcc ccatggccca ggcaggcagc 1140
aggtctgggg caggaggggg gttgtggagt gccttgactc ggggcctggc ccccccatct 1200
ctgtcttgca ggacaattgc cgtcttctgt ctcgtggggc atcctcctgc tggcaggcct 1260
gtgctgcctg gtccctgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct 1320
gtgcctgctg aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct 1380
gagctgggac tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc 1440
cagagtgccc aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt 1500
ggagttcact gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct 1560
gggccccacc atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc 1620
cagccaccct gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc 1680
tgagtatgat gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg 1740
cagccacacc tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct 1800
gtgcctgacc tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct 1860
gattggggcc ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct 1920
gcacaagttc atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac 1980
caagaacagc ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca 2040
cactgtgaat ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc 2100
tgtgtactgg catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga 2160
gggccacacc ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac 2220
cttcctgact gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat 2280
cagcagccac cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga 2340
gccccagctg aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga 2400
ctctgagatg gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag 2460
gtctgtggcc aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga 2520
ctgggactat gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct 2580
gaacaatggc ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac 2640
tgatgaaacc ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct 2700
gctgtatggg gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc 2760
ctacaacatc tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc 2820
caagggggtg aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa 2880
gtggactgtg actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata 2940
ctacagcagc tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct 3000
gatctgctac aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa 3060
tgtgatcctg ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca 3120
gaggttcctg cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa 3180
catcatgcac agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca 3240
tgaggtggcc tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt 3300
cttctctggc tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc 3360
cttctctggg gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg 3420
ccacaactct gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga 3480
caagaacact ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag 3540
caagaacaat gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca 3600
ccagagggag atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga 3660
caccatctct gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca 3720
gagccccagg agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct 3780
gtgggactat ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc 3840
tgtgccccag ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc 3900
cctgtacaga ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga 3960
ggtggaggac aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta 4020
cagcagcctg atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt 4080
tgtgaagccc aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac 4140
caaggatgag tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga 4200
tgtgcactct ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc 4260
ccatggcagg caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac 4320
caagagctgg tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca 4380
gatggaggac cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat 4440
ggacaccctg cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag 4500
catgggcagc aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag 4560
gaagaaggag gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt 4620
ggagatgctg cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct 4680
gcatgctggc atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg 4740
catggcctct ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg 4800
ggcccccaag ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga 4860
gcccttcagc tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac 4920
ccagggggcc aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag 4980
cctggatggc aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt 5040
ctttggcaat gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc 5100
cagatacatc aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct 5160
gatgggctgt gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc 5220
tgatgcccag atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag 5280
caaggccagg ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc 5340
caaggagtgg ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca 5400
gggggtgaag agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca 5460
ggatggccac cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa 5520
ccaggacagc ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct 5580
gaggattcac ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg 5640
tgaggcccag gacctgtact gacctcgagg tgtgccttct agttgccagc catctgttgt 5700
ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 5760
ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 5820
ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 5880
ggtgggctct atgggcacgt ggcggccgca ggaaccccta gtgatggagt tggccactcc 5940
ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 6000
ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaa 6054
<210> 30
<211> 6054
<212> DNA
<213> 腺相关病毒2
<400> 30
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgaagc ctctcctggg ggtggggaga ggcccagagg 540
ataagtccag gatgttgatt tcttctcctg cctccaggaa tgtttgtgct cctgagatta 600
ttaaccagag caaacagaga gagtaaatgc tgccaagggc cacttggact tttgactgct 660
ttttaaaaat taacctgagc ctctcctggg ggtggggaga ggcccagagg ataagtccag 720
gatgttgatt tcttctcctg cctccaggaa tgtttgtgct cctgagatta ttaaccagag 780
caaacagaga gagtaaatgc tgccaagggc cacttggact tttgactgct ttttaaaaat 840
taacctggtc gactggacac aggacgctgt ggtttctgag ccagggggcg actcagatcc 900
cagccagtgg acttagcccc tgtttgctcc tccgataact ggggtgacct tggttaatat 960
tcaccagcag cctcccccgt tgcccctctg gatccactgc ttaaatacgg acgaggacag 1020
ggccctgtct cctcagcttc aggcaccacc actgacctgg gacagtgaat cgtaagtatg 1080
cctttcactg cgagaggttc tggagaggct tctgagctcc ccatggccca ggcaggcagc 1140
aggtctgggg caggaggggg gttgtggagt gccttgactc ggggcctggc ccccccatct 1200
ctgtcttgca ggacaattgc cgtcttctgt ctcgtggggc atcctcctgc tggcaggcct 1260
gtgctgcctg gtccctgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct 1320
gtgcctgctg aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct 1380
gagctgggac tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc 1440
cagagtgccc aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt 1500
ggagttcact gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct 1560
gggccccacc atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc 1620
cagccaccct gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc 1680
tgagtatgat gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg 1740
cagccacacc tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct 1800
gtgcctgacc tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct 1860
gattggggcc ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct 1920
gcacaagttc atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac 1980
caagaacagc ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca 2040
cactgtgaat ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc 2100
tgtgtactgg catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga 2160
gggccacacc ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac 2220
cttcctgact gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat 2280
cagcagccac cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga 2340
gccccagctg aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga 2400
ctctgagatg gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag 2460
gtctgtggcc aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga 2520
ctgggactat gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct 2580
gaacaatggc ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac 2640
tgatgaaacc ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct 2700
gctgtatggg gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc 2760
ctacaacatc tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc 2820
caagggggtg aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa 2880
gtggactgtg actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata 2940
ctacagcagc tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct 3000
gatctgctac aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa 3060
tgtgatcctg ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca 3120
gaggttcctg cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa 3180
catcatgcac agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca 3240
tgaggtggcc tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt 3300
cttctctggc tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc 3360
cttctctggg gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg 3420
ccacaactct gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga 3480
caagaacact ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag 3540
caagaacaat gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca 3600
ccagagggag atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga 3660
caccatctct gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca 3720
gagccccagg agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct 3780
gtgggactat ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc 3840
tgtgccccag ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc 3900
cctgtacaga ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga 3960
ggtggaggac aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta 4020
cagcagcctg atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt 4080
tgtgaagccc aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac 4140
caaggatgag tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga 4200
tgtgcactct ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc 4260
ccatggcagg caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac 4320
caagagctgg tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca 4380
gatggaggac cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat 4440
ggacaccctg cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag 4500
catgggcagc aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag 4560
gaagaaggag gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt 4620
ggagatgctg cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct 4680
gcatgctggc atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg 4740
catggcctct ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg 4800
ggcccccaag ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga 4860
gcccttcagc tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac 4920
ccagggggcc aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag 4980
cctggatggc aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt 5040
ctttggcaat gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc 5100
cagatacatc aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct 5160
gatgggctgt gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc 5220
tgatgcccag atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag 5280
caaggccagg ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc 5340
caaggagtgg ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca 5400
gggggtgaag agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca 5460
ggatggccac cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa 5520
ccaggacagc ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct 5580
gaggattcac ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg 5640
tgaggcccag gacctgtact gacctcgagg tgtgccttct agttgccagc catctgttgt 5700
ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 5760
ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 5820
ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 5880
ggtgggctct atgggcacgt ggcggccgca ggaaccccta gtgatggagt tggccactcc 5940
ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 6000
ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaa 6054
<210> 31
<211> 5504
<212> DNA
<213> 腺相关病毒2
<400> 31
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgactg gacacaggac gctgtggttt ctgagccagg 540
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 600
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 660
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 720
tgaatcgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct gtgcctgctg 780
aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct gagctgggac 840
tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc cagagtgccc 900
aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt ggagttcact 960
gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct gggccccacc 1020
atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc cagccaccct 1080
gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc tgagtatgat 1140
gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg cagccacacc 1200
tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct gtgcctgacc 1260
tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct gattggggcc 1320
ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct gcacaagttc 1380
atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac caagaacagc 1440
ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca cactgtgaat 1500
ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc tgtgtactgg 1560
catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga gggccacacc 1620
ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac cttcctgact 1680
gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat cagcagccac 1740
cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga gccccagctg 1800
aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga ctctgagatg 1860
gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag gtctgtggcc 1920
aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga ctgggactat 1980
gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct gaacaatggc 2040
ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac tgatgaaacc 2100
ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct gctgtatggg 2160
gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc ctacaacatc 2220
tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc caagggggtg 2280
aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa gtggactgtg 2340
actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata ctacagcagc 2400
tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct gatctgctac 2460
aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa tgtgatcctg 2520
ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca gaggttcctg 2580
cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa catcatgcac 2640
agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc 2700
tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt cttctctggc 2760
tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc cttctctggg 2820
gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg ccacaactct 2880
gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga caagaacact 2940
ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag caagaacaat 3000
gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca ccagagggag 3060
atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga caccatctct 3120
gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca gagccccagg 3180
agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct gtgggactat 3240
ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc tgtgccccag 3300
ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc cctgtacaga 3360
ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga ggtggaggac 3420
aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta cagcagcctg 3480
atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt tgtgaagccc 3540
aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac caaggatgag 3600
tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga tgtgcactct 3660
ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc ccatggcagg 3720
caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac caagagctgg 3780
tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca gatggaggac 3840
cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat ggacaccctg 3900
cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag catgggcagc 3960
aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag gaagaaggag 4020
gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt ggagatgctg 4080
cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct gcatgctggc 4140
atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg catggcctct 4200
ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg ggcccccaag 4260
ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga gcccttcagc 4320
tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac ccagggggcc 4380
aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag cctggatggc 4440
aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt ctttggcaat 4500
gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc cagatacatc 4560
aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct gatgggctgt 4620
gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc tgatgcccag 4680
atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag caaggccagg 4740
ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc caaggagtgg 4800
ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca gggggtgaag 4860
agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca ggatggccac 4920
cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa ccaggacagc 4980
ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct gaggattcac 5040
ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg tgaggcccag 5100
gacctgtact gacctcgagg tgtgccttct agttgccagc catctgttgt ttgcccctcc 5160
cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 5220
gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 5280
gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 5340
atgggcacgt ggcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc 5400
gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg 5460
gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaa 5504
<210> 32
<211> 5507
<212> DNA
<213> 腺相关病毒2
<400> 32
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgaccc ctctcacact 180
acctaaacca cgccaggaca acctctgctc ctctccaccg aaattccaag gggtcgagtg 240
gatgttggag gtggcatggg cccagagagg tctctgacct ctgccccagc tccaaggtca 300
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 360
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagctgct 420
ggaggatggg aactgagggg ttggaagggg gcagggtgag cccagaaact cctgtgtgcc 480
tctgagcctg cagacgcgaa acgtcgacga tcttgctacc agtggaacag ccactaagga 540
ttctgcagtg agagcagagg gccagctaag tggtactctc ccagagactg tctgactcac 600
gccaccccct ccaccttgga cacaggacgc tgtggtttct gagccaggta caatgactcc 660
tttcggtaag tgcagtggaa gctgtacact gcccaggcaa agcgtccggg cagcgtaggc 720
gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga taactggggt 780
gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc actgcttaaa 840
tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga cctgggacag 900
tgaatcgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct gtgcctgctg 960
aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct gagctgggac 1020
tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc cagagtgccc 1080
aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt ggagttcact 1140
gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct gggccccacc 1200
atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc cagccaccct 1260
gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc tgagtatgat 1320
gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg cagccacacc 1380
tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct gtgcctgacc 1440
tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct gattggggcc 1500
ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct gcacaagttc 1560
atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac caagaacagc 1620
ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca cactgtgaat 1680
ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc tgtgtactgg 1740
catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga gggccacacc 1800
ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac cttcctgact 1860
gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat cagcagccac 1920
cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga gccccagctg 1980
aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga ctctgagatg 2040
gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag gtctgtggcc 2100
aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga ctgggactat 2160
gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct gaacaatggc 2220
ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac tgatgaaacc 2280
ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct gctgtatggg 2340
gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc ctacaacatc 2400
tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc caagggggtg 2460
aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa gtggactgtg 2520
actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata ctacagcagc 2580
tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct gatctgctac 2640
aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa tgtgatcctg 2700
ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca gaggttcctg 2760
cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa catcatgcac 2820
agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca tgaggtggcc 2880
tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt cttctctggc 2940
tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc cttctctggg 3000
gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg ccacaactct 3060
gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga caagaacact 3120
ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag caagaacaat 3180
gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca ccagagggag 3240
atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga caccatctct 3300
gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca gagccccagg 3360
agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct gtgggactat 3420
ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc tgtgccccag 3480
ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc cctgtacaga 3540
ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga ggtggaggac 3600
aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta cagcagcctg 3660
atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt tgtgaagccc 3720
aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac caaggatgag 3780
tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga tgtgcactct 3840
ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc ccatggcagg 3900
caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac caagagctgg 3960
tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca gatggaggac 4020
cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat ggacaccctg 4080
cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag catgggcagc 4140
aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag gaagaaggag 4200
gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt ggagatgctg 4260
cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct gcatgctggc 4320
atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg catggcctct 4380
ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg ggcccccaag 4440
ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga gcccttcagc 4500
tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac ccagggggcc 4560
aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag cctggatggc 4620
aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt ctttggcaat 4680
gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc cagatacatc 4740
aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct gatgggctgt 4800
gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc tgatgcccag 4860
atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag caaggccagg 4920
ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc caaggagtgg 4980
ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca gggggtgaag 5040
agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca ggatggccac 5100
cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa ccaggacagc 5160
ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct gaggattcac 5220
ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg tgaggcccag 5280
gacctgtact gacctcgagg aataaaggaa atttattttc attgcaatag tgtgttggtt 5340
ttttgtgtca cgtggcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 5400
cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 5460
cgggcggcct cagtgagcga gcgagcgcgc agagagggag tggccaa 5507
<210> 33
<211> 5311
<212> DNA
<213> 腺相关病毒2
<400> 33
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgacgc agagaggtct 180
ctgacctctg ccccagctcc aaggtcagca ggcagggagg gctgtgtgtt tgctgtttgc 240
tgcttgcaat gtttgcccat tttagggaca tgagtaggct gaagtttgtt cagtgtggac 300
ttcagaggca gcacacaaac agccagagag gtctctgacc tctgccccag ctccaaggtc 360
agcaggcagg gagggctgtg tgtttgctgt ttgctgcttg caatgtttgc ccattttagg 420
gacatgagta ggctgaagtt tgttcagtgt ggacttcaga ggcagcacac aaacagcacg 480
cgaaacgtcg actggacaca ggacgctgtg gtttctgagc cagggggcga ctcagatccc 540
agccagtgga cttagcccct gtttgctcct ccgataactg gggtgacctt ggttaatatt 600
caccagcagc ctcccccgtt gcccctctgg atccactgct taaatacgga cgaggacagg 660
gccctgtctc ctcagcttca ggcaccacca ctgacctggg acagtgaatc gcgatcgcca 720
ccatgcagat tgagctgagc acctgcttct tcctgtgcct gctgaggttc tgcttctctg 780
ccaccaggag atactacctg ggggctgtgg agctgagctg ggactacatg cagtctgacc 840
tgggggagct gcctgtggat gccaggttcc cccccagagt gcccaagagc ttccccttca 900
acacctctgt ggtgtacaag aagaccctgt ttgtggagtt cactgaccac ctgttcaaca 960
ttgccaagcc caggcccccc tggatgggcc tgctgggccc caccatccag gctgaggtgt 1020
atgacactgt ggtgatcacc ctgaagaaca tggccagcca ccctgtgagc ctgcatgctg 1080
tgggggtgag ctactggaag gcctctgagg gggctgagta tgatgaccag accagccaga 1140
gggagaagga ggatgacaag gtgttccctg ggggcagcca cacctatgtg tggcaggtgc 1200
tgaaggagaa tggccccatg gcctctgacc ccctgtgcct gacctacagc tacctgagcc 1260
atgtggacct ggtgaaggac ctgaactctg gcctgattgg ggccctgctg gtgtgcaggg 1320
agggcagcct ggccaaggag aagacccaga ccctgcacaa gttcatcctg ctgtttgctg 1380
tgtttgatga gggcaagagc tggcactctg aaaccaagaa cagcctgatg caggacaggg 1440
atgctgcctc tgccagggcc tggcccaaga tgcacactgt gaatggctat gtgaacagga 1500
gcctgcctgg cctgattggc tgccacagga agtctgtgta ctggcatgtg attggcatgg 1560
gcaccacccc tgaggtgcac agcatcttcc tggagggcca caccttcctg gtcaggaacc 1620
acaggcaggc cagcctggag atcagcccca tcaccttcct gactgcccag accctgctga 1680
tggacctggg ccagttcctg ctgttctgcc acatcagcag ccaccagcat gatggcatgg 1740
aggcctatgt gaaggtggac agctgccctg aggagcccca gctgaggatg aagaacaatg 1800
aggaggctga ggactatgat gatgacctga ctgactctga gatggatgtg gtgaggtttg 1860
atgatgacaa cagccccagc ttcatccaga tcaggtctgt ggccaagaag caccccaaga 1920
cctgggtgca ctacattgct gctgaggagg aggactggga ctatgccccc ctggtgctgg 1980
cccctgatga caggagctac aagagccagt acctgaacaa tggcccccag aggattggca 2040
ggaagtacaa gaaggtcagg ttcatggcct acactgatga aaccttcaag accagggagg 2100
ccatccagca tgagtctggc atcctgggcc ccctgctgta tggggaggtg ggggacaccc 2160
tgctgatcat cttcaagaac caggccagca ggccctacaa catctacccc catggcatca 2220
ctgatgtgag gcccctgtac agcaggaggc tgcccaaggg ggtgaagcac ctgaaggact 2280
tccccatcct gcctggggag atcttcaagt acaagtggac tgtgactgtg gaggatggcc 2340
ccaccaagtc tgaccccagg tgcctgacca gatactacag cagctttgtg aacatggaga 2400
gggacctggc ctctggcctg attggccccc tgctgatctg ctacaaggag tctgtggacc 2460
agaggggcaa ccagatcatg tctgacaaga ggaatgtgat cctgttctct gtgtttgatg 2520
agaacaggag ctggtacctg actgagaaca tccagaggtt cctgcccaac cctgctgggg 2580
tgcagctgga ggaccctgag ttccaggcca gcaacatcat gcacagcatc aatggctatg 2640
tgtttgacag cctgcagctg tctgtgtgcc tgcatgaggt ggcctactgg tacatcctga 2700
gcattggggc ccagactgac ttcctgtctg tgttcttctc tggctacacc ttcaagcaca 2760
agatggtgta tgaggacacc ctgaccctgt tccccttctc tggggagact gtgttcatga 2820
gcatggagaa ccctggcctg tggattctgg gctgccacaa ctctgacttc aggaacaggg 2880
gcatgactgc cctgctgaaa gtctccagct gtgacaagaa cactggggac tactatgagg 2940
acagctatga ggacatctct gcctacctgc tgagcaagaa caatgccatt gagcccagga 3000
gcttcagcca gaacccccca gtgctgaaga ggcaccagag ggagatcacc aggaccaccc 3060
tgcagtctga ccaggaggag attgactatg atgacaccat ctctgtggag atgaagaagg 3120
aggactttga catctacgac gaggacgaga accagagccc caggagcttc cagaagaaga 3180
ccaggcacta cttcattgct gctgtggaga ggctgtggga ctatggcatg agcagcagcc 3240
cccatgtgct gaggaacagg gcccagtctg gctctgtgcc ccagttcaag aaggtggtgt 3300
tccaggagtt cactgatggc agcttcaccc agcccctgta cagaggggag ctgaatgagc 3360
acctgggcct gctgggcccc tacatcaggg ctgaggtgga ggacaacatc atggtgacct 3420
tcaggaacca ggccagcagg ccctacagct tctacagcag cctgatcagc tatgaggagg 3480
accagaggca gggggctgag cccaggaaga actttgtgaa gcccaatgaa accaagacct 3540
acttctggaa ggtgcagcac cacatggccc ccaccaagga tgagtttgac tgcaaggcct 3600
gggcctactt ctctgatgtg gacctggaga aggatgtgca ctctggcctg attggccccc 3660
tgctggtgtg ccacaccaac accctgaacc ctgcccatgg caggcaggtg actgtgcagg 3720
agtttgccct gttcttcacc atctttgatg aaaccaagag ctggtacttc actgagaaca 3780
tggagaggaa ctgcagggcc ccctgcaaca tccagatgga ggaccccacc ttcaaggaga 3840
actacaggtt ccatgccatc aatggctaca tcatggacac cctgcctggc ctggtgatgg 3900
cccaggacca gaggatcagg tggtacctgc tgagcatggg cagcaatgag aacatccaca 3960
gcatccactt ctctggccat gtgttcactg tgaggaagaa ggaggagtac aagatggccc 4020
tgtacaacct gtaccctggg gtgtttgaga ctgtggagat gctgcccagc aaggctggca 4080
tctggagggt ggagtgcctg attggggagc acctgcatgc tggcatgagc accctgttcc 4140
tggtgtacag caacaagtgc cagacccccc tgggcatggc ctctggccac atcagggact 4200
tccagatcac tgcctctggc cagtatggcc agtgggcccc caagctggcc aggctgcact 4260
actctggcag catcaatgcc tggagcacca aggagccctt cagctggatc aaggtggacc 4320
tgctggcccc catgatcatc catggcatca agacccaggg ggccaggcag aagttcagca 4380
gcctgtacat cagccagttc atcatcatgt acagcctgga tggcaagaag tggcagacct 4440
acaggggcaa cagcactggc accctgatgg tgttctttgg caatgtggac agctctggca 4500
tcaagcacaa catcttcaac ccccccatca ttgccagata catcaggctg caccccaccc 4560
actacagcat caggagcacc ctgaggatgg agctgatggg ctgtgacctg aacagctgca 4620
gcatgcccct gggcatggag agcaaggcca tctctgatgc ccagatcact gccagcagct 4680
acttcaccaa catgtttgcc acctggagcc ccagcaaggc caggctgcac ctgcagggca 4740
ggagcaatgc ctggaggccc caggtcaaca accccaagga gtggctgcag gtggacttcc 4800
agaagaccat gaaggtgact ggggtgacca cccagggggt gaagagcctg ctgaccagca 4860
tgtatgtgaa ggagttcctg atcagcagca gccaggatgg ccaccagtgg accctgttct 4920
tccagaatgg caaggtgaag gtgttccagg gcaaccagga cagcttcacc cctgtggtga 4980
acagcctgga cccccccctg ctgaccagat acctgaggat tcacccccag agctgggtgc 5040
accagattgc cctgaggatg gaggtgctgg gctgtgaggc ccaggacctg tactgacctc 5100
gaggaataaa ggaaatttat tttcattgca atagtgtgtt ggttttttgt gtcacgtggc 5160
ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca 5220
ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga 5280
gcgagcgagc gcgcagagag ggagtggcca a 5311
<210> 34
<211> 5156
<212> DNA
<213> 腺相关病毒2
<400> 34
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgacca gagaggtctc 180
tgacctctgc cccagctcca aggtcagcag gcagggaggg ctgtgtgttt gctgtttgct 240
gcttgcaatg tttgcccatt ttagggacat gagtaggctg aagtttgttc agtgtggact 300
tcagaggcag cacacaaaca gcacgcgaaa cgtcgactgg acacaggacg ctgtggtttc 360
tgagccaggg ggcgactcag atcccagcca gtggacttag cccctgtttg ctcctccgat 420
aactggggtg accttggtta atattcacca gcagcctccc ccgttgcccc tctggatcca 480
ctgcttaaat acggacgagg acagggccct gtctcctcag cttcaggcac caccactgac 540
ctgggacagt gaatcgcgat cgccaccatg cagattgagc tgagcacctg cttcttcctg 600
tgcctgctga ggttctgctt ctctgccacc aggagatact acctgggggc tgtggagctg 660
agctgggact acatgcagtc tgacctgggg gagctgcctg tggatgccag gttccccccc 720
agagtgccca agagcttccc cttcaacacc tctgtggtgt acaagaagac cctgtttgtg 780
gagttcactg accacctgtt caacattgcc aagcccaggc ccccctggat gggcctgctg 840
ggccccacca tccaggctga ggtgtatgac actgtggtga tcaccctgaa gaacatggcc 900
agccaccctg tgagcctgca tgctgtgggg gtgagctact ggaaggcctc tgagggggct 960
gagtatgatg accagaccag ccagagggag aaggaggatg acaaggtgtt ccctgggggc 1020
agccacacct atgtgtggca ggtgctgaag gagaatggcc ccatggcctc tgaccccctg 1080
tgcctgacct acagctacct gagccatgtg gacctggtga aggacctgaa ctctggcctg 1140
attggggccc tgctggtgtg cagggagggc agcctggcca aggagaagac ccagaccctg 1200
cacaagttca tcctgctgtt tgctgtgttt gatgagggca agagctggca ctctgaaacc 1260
aagaacagcc tgatgcagga cagggatgct gcctctgcca gggcctggcc caagatgcac 1320
actgtgaatg gctatgtgaa caggagcctg cctggcctga ttggctgcca caggaagtct 1380
gtgtactggc atgtgattgg catgggcacc acccctgagg tgcacagcat cttcctggag 1440
ggccacacct tcctggtcag gaaccacagg caggccagcc tggagatcag ccccatcacc 1500
ttcctgactg cccagaccct gctgatggac ctgggccagt tcctgctgtt ctgccacatc 1560
agcagccacc agcatgatgg catggaggcc tatgtgaagg tggacagctg ccctgaggag 1620
ccccagctga ggatgaagaa caatgaggag gctgaggact atgatgatga cctgactgac 1680
tctgagatgg atgtggtgag gtttgatgat gacaacagcc ccagcttcat ccagatcagg 1740
tctgtggcca agaagcaccc caagacctgg gtgcactaca ttgctgctga ggaggaggac 1800
tgggactatg cccccctggt gctggcccct gatgacagga gctacaagag ccagtacctg 1860
aacaatggcc cccagaggat tggcaggaag tacaagaagg tcaggttcat ggcctacact 1920
gatgaaacct tcaagaccag ggaggccatc cagcatgagt ctggcatcct gggccccctg 1980
ctgtatgggg aggtggggga caccctgctg atcatcttca agaaccaggc cagcaggccc 2040
tacaacatct acccccatgg catcactgat gtgaggcccc tgtacagcag gaggctgccc 2100
aagggggtga agcacctgaa ggacttcccc atcctgcctg gggagatctt caagtacaag 2160
tggactgtga ctgtggagga tggccccacc aagtctgacc ccaggtgcct gaccagatac 2220
tacagcagct ttgtgaacat ggagagggac ctggcctctg gcctgattgg ccccctgctg 2280
atctgctaca aggagtctgt ggaccagagg ggcaaccaga tcatgtctga caagaggaat 2340
gtgatcctgt tctctgtgtt tgatgagaac aggagctggt acctgactga gaacatccag 2400
aggttcctgc ccaaccctgc tggggtgcag ctggaggacc ctgagttcca ggccagcaac 2460
atcatgcaca gcatcaatgg ctatgtgttt gacagcctgc agctgtctgt gtgcctgcat 2520
gaggtggcct actggtacat cctgagcatt ggggcccaga ctgacttcct gtctgtgttc 2580
ttctctggct acaccttcaa gcacaagatg gtgtatgagg acaccctgac cctgttcccc 2640
ttctctgggg agactgtgtt catgagcatg gagaaccctg gcctgtggat tctgggctgc 2700
cacaactctg acttcaggaa caggggcatg actgccctgc tgaaagtctc cagctgtgac 2760
aagaacactg gggactacta tgaggacagc tatgaggaca tctctgccta cctgctgagc 2820
aagaacaatg ccattgagcc caggagcttc agccagaacc ccccagtgct gaagaggcac 2880
cagagggaga tcaccaggac caccctgcag tctgaccagg aggagattga ctatgatgac 2940
accatctctg tggagatgaa gaaggaggac tttgacatct acgacgagga cgagaaccag 3000
agccccagga gcttccagaa gaagaccagg cactacttca ttgctgctgt ggagaggctg 3060
tgggactatg gcatgagcag cagcccccat gtgctgagga acagggccca gtctggctct 3120
gtgccccagt tcaagaaggt ggtgttccag gagttcactg atggcagctt cacccagccc 3180
ctgtacagag gggagctgaa tgagcacctg ggcctgctgg gcccctacat cagggctgag 3240
gtggaggaca acatcatggt gaccttcagg aaccaggcca gcaggcccta cagcttctac 3300
agcagcctga tcagctatga ggaggaccag aggcaggggg ctgagcccag gaagaacttt 3360
gtgaagccca atgaaaccaa gacctacttc tggaaggtgc agcaccacat ggcccccacc 3420
aaggatgagt ttgactgcaa ggcctgggcc tacttctctg atgtggacct ggagaaggat 3480
gtgcactctg gcctgattgg ccccctgctg gtgtgccaca ccaacaccct gaaccctgcc 3540
catggcaggc aggtgactgt gcaggagttt gccctgttct tcaccatctt tgatgaaacc 3600
aagagctggt acttcactga gaacatggag aggaactgca gggccccctg caacatccag 3660
atggaggacc ccaccttcaa ggagaactac aggttccatg ccatcaatgg ctacatcatg 3720
gacaccctgc ctggcctggt gatggcccag gaccagagga tcaggtggta cctgctgagc 3780
atgggcagca atgagaacat ccacagcatc cacttctctg gccatgtgtt cactgtgagg 3840
aagaaggagg agtacaagat ggccctgtac aacctgtacc ctggggtgtt tgagactgtg 3900
gagatgctgc ccagcaaggc tggcatctgg agggtggagt gcctgattgg ggagcacctg 3960
catgctggca tgagcaccct gttcctggtg tacagcaaca agtgccagac ccccctgggc 4020
atggcctctg gccacatcag ggacttccag atcactgcct ctggccagta tggccagtgg 4080
gcccccaagc tggccaggct gcactactct ggcagcatca atgcctggag caccaaggag 4140
cccttcagct ggatcaaggt ggacctgctg gcccccatga tcatccatgg catcaagacc 4200
cagggggcca ggcagaagtt cagcagcctg tacatcagcc agttcatcat catgtacagc 4260
ctggatggca agaagtggca gacctacagg ggcaacagca ctggcaccct gatggtgttc 4320
tttggcaatg tggacagctc tggcatcaag cacaacatct tcaacccccc catcattgcc 4380
agatacatca ggctgcaccc cacccactac agcatcagga gcaccctgag gatggagctg 4440
atgggctgtg acctgaacag ctgcagcatg cccctgggca tggagagcaa ggccatctct 4500
gatgcccaga tcactgccag cagctacttc accaacatgt ttgccacctg gagccccagc 4560
aaggccaggc tgcacctgca gggcaggagc aatgcctgga ggccccaggt caacaacccc 4620
aaggagtggc tgcaggtgga cttccagaag accatgaagg tgactggggt gaccacccag 4680
ggggtgaaga gcctgctgac cagcatgtat gtgaaggagt tcctgatcag cagcagccag 4740
gatggccacc agtggaccct gttcttccag aatggcaagg tgaaggtgtt ccagggcaac 4800
caggacagct tcacccctgt ggtgaacagc ctggaccccc ccctgctgac cagatacctg 4860
aggattcacc cccagagctg ggtgcaccag attgccctga ggatggaggt gctgggctgt 4920
gaggcccagg acctgtactg acctcgagga ataaaggaaa tttattttca ttgcaatagt 4980
gtgttggttt tttgtgtcac gtggcggccg caggaacccc tagtgatgga gttggccact 5040
ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg 5100
ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gagagggagt ggccaa 5156
<210> 35
<211> 5178
<212> DNA
<213> 腺相关病毒2
<400> 35
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgact 360
ggacacagga cgctgtggtt tctgagccag ggggcgactc agatcccagc cagtggactt 420
agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc 480
ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc 540
agcttcaggc accaccactg acctgggaca gtgaatcgcg atcgccacca tgcagattga 600
gctgagcacc tgcttcttcc tgtgcctgct gaggttctgc ttctctgcca ccaggagata 660
ctacctgggg gctgtggagc tgagctggga ctacatgcag tctgacctgg gggagctgcc 720
tgtggatgcc aggttccccc ccagagtgcc caagagcttc cccttcaaca cctctgtggt 780
gtacaagaag accctgtttg tggagttcac tgaccacctg ttcaacattg ccaagcccag 840
gcccccctgg atgggcctgc tgggccccac catccaggct gaggtgtatg acactgtggt 900
gatcaccctg aagaacatgg ccagccaccc tgtgagcctg catgctgtgg gggtgagcta 960
ctggaaggcc tctgaggggg ctgagtatga tgaccagacc agccagaggg agaaggagga 1020
tgacaaggtg ttccctgggg gcagccacac ctatgtgtgg caggtgctga aggagaatgg 1080
ccccatggcc tctgaccccc tgtgcctgac ctacagctac ctgagccatg tggacctggt 1140
gaaggacctg aactctggcc tgattggggc cctgctggtg tgcagggagg gcagcctggc 1200
caaggagaag acccagaccc tgcacaagtt catcctgctg tttgctgtgt ttgatgaggg 1260
caagagctgg cactctgaaa ccaagaacag cctgatgcag gacagggatg ctgcctctgc 1320
cagggcctgg cccaagatgc acactgtgaa tggctatgtg aacaggagcc tgcctggcct 1380
gattggctgc cacaggaagt ctgtgtactg gcatgtgatt ggcatgggca ccacccctga 1440
ggtgcacagc atcttcctgg agggccacac cttcctggtc aggaaccaca ggcaggccag 1500
cctggagatc agccccatca ccttcctgac tgcccagacc ctgctgatgg acctgggcca 1560
gttcctgctg ttctgccaca tcagcagcca ccagcatgat ggcatggagg cctatgtgaa 1620
ggtggacagc tgccctgagg agccccagct gaggatgaag aacaatgagg aggctgagga 1680
ctatgatgat gacctgactg actctgagat ggatgtggtg aggtttgatg atgacaacag 1740
ccccagcttc atccagatca ggtctgtggc caagaagcac cccaagacct gggtgcacta 1800
cattgctgct gaggaggagg actgggacta tgcccccctg gtgctggccc ctgatgacag 1860
gagctacaag agccagtacc tgaacaatgg cccccagagg attggcagga agtacaagaa 1920
ggtcaggttc atggcctaca ctgatgaaac cttcaagacc agggaggcca tccagcatga 1980
gtctggcatc ctgggccccc tgctgtatgg ggaggtgggg gacaccctgc tgatcatctt 2040
caagaaccag gccagcaggc cctacaacat ctacccccat ggcatcactg atgtgaggcc 2100
cctgtacagc aggaggctgc ccaagggggt gaagcacctg aaggacttcc ccatcctgcc 2160
tggggagatc ttcaagtaca agtggactgt gactgtggag gatggcccca ccaagtctga 2220
ccccaggtgc ctgaccagat actacagcag ctttgtgaac atggagaggg acctggcctc 2280
tggcctgatt ggccccctgc tgatctgcta caaggagtct gtggaccaga ggggcaacca 2340
gatcatgtct gacaagagga atgtgatcct gttctctgtg tttgatgaga acaggagctg 2400
gtacctgact gagaacatcc agaggttcct gcccaaccct gctggggtgc agctggagga 2460
ccctgagttc caggccagca acatcatgca cagcatcaat ggctatgtgt ttgacagcct 2520
gcagctgtct gtgtgcctgc atgaggtggc ctactggtac atcctgagca ttggggccca 2580
gactgacttc ctgtctgtgt tcttctctgg ctacaccttc aagcacaaga tggtgtatga 2640
ggacaccctg accctgttcc ccttctctgg ggagactgtg ttcatgagca tggagaaccc 2700
tggcctgtgg attctgggct gccacaactc tgacttcagg aacaggggca tgactgccct 2760
gctgaaagtc tccagctgtg acaagaacac tggggactac tatgaggaca gctatgagga 2820
catctctgcc tacctgctga gcaagaacaa tgccattgag cccaggagct tcagccagaa 2880
ccccccagtg ctgaagaggc accagaggga gatcaccagg accaccctgc agtctgacca 2940
ggaggagatt gactatgatg acaccatctc tgtggagatg aagaaggagg actttgacat 3000
ctacgacgag gacgagaacc agagccccag gagcttccag aagaagacca ggcactactt 3060
cattgctgct gtggagaggc tgtgggacta tggcatgagc agcagccccc atgtgctgag 3120
gaacagggcc cagtctggct ctgtgcccca gttcaagaag gtggtgttcc aggagttcac 3180
tgatggcagc ttcacccagc ccctgtacag aggggagctg aatgagcacc tgggcctgct 3240
gggcccctac atcagggctg aggtggagga caacatcatg gtgaccttca ggaaccaggc 3300
cagcaggccc tacagcttct acagcagcct gatcagctat gaggaggacc agaggcaggg 3360
ggctgagccc aggaagaact ttgtgaagcc caatgaaacc aagacctact tctggaaggt 3420
gcagcaccac atggccccca ccaaggatga gtttgactgc aaggcctggg cctacttctc 3480
tgatgtggac ctggagaagg atgtgcactc tggcctgatt ggccccctgc tggtgtgcca 3540
caccaacacc ctgaaccctg cccatggcag gcaggtgact gtgcaggagt ttgccctgtt 3600
cttcaccatc tttgatgaaa ccaagagctg gtacttcact gagaacatgg agaggaactg 3660
cagggccccc tgcaacatcc agatggagga ccccaccttc aaggagaact acaggttcca 3720
tgccatcaat ggctacatca tggacaccct gcctggcctg gtgatggccc aggaccagag 3780
gatcaggtgg tacctgctga gcatgggcag caatgagaac atccacagca tccacttctc 3840
tggccatgtg ttcactgtga ggaagaagga ggagtacaag atggccctgt acaacctgta 3900
ccctggggtg tttgagactg tggagatgct gcccagcaag gctggcatct ggagggtgga 3960
gtgcctgatt ggggagcacc tgcatgctgg catgagcacc ctgttcctgg tgtacagcaa 4020
caagtgccag acccccctgg gcatggcctc tggccacatc agggacttcc agatcactgc 4080
ctctggccag tatggccagt gggcccccaa gctggccagg ctgcactact ctggcagcat 4140
caatgcctgg agcaccaagg agcccttcag ctggatcaag gtggacctgc tggcccccat 4200
gatcatccat ggcatcaaga cccagggggc caggcagaag ttcagcagcc tgtacatcag 4260
ccagttcatc atcatgtaca gcctggatgg caagaagtgg cagacctaca ggggcaacag 4320
cactggcacc ctgatggtgt tctttggcaa tgtggacagc tctggcatca agcacaacat 4380
cttcaacccc cccatcattg ccagatacat caggctgcac cccacccact acagcatcag 4440
gagcaccctg aggatggagc tgatgggctg tgacctgaac agctgcagca tgcccctggg 4500
catggagagc aaggccatct ctgatgccca gatcactgcc agcagctact tcaccaacat 4560
gtttgccacc tggagcccca gcaaggccag gctgcacctg cagggcagga gcaatgcctg 4620
gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga agaccatgaa 4680
ggtgactggg gtgaccaccc agggggtgaa gagcctgctg accagcatgt atgtgaagga 4740
gttcctgatc agcagcagcc aggatggcca ccagtggacc ctgttcttcc agaatggcaa 4800
ggtgaaggtg ttccagggca accaggacag cttcacccct gtggtgaaca gcctggaccc 4860
ccccctgctg accagatacc tgaggattca cccccagagc tgggtgcacc agattgccct 4920
gaggatggag gtgctgggct gtgaggccca ggacctgtac tgacctcgag gaataaagga 4980
aatttatttt cattgcaata gtgtgttggt tttttgtgtc acgtggcggc cgcaggaacc 5040
cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 5100
accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 5160
cagagaggga gtggccaa 5178
<210> 36
<211> 5160
<212> DNA
<213> 腺相关病毒2
<400> 36
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtt gtccctaaaa tgggcaaaca 180
ttgcaagcag caaacagcaa acatgtccct aaaatgggca aacattgcaa gcagcaaaca 240
gcaaacatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac atgtccctaa 300
aatgggcaaa cattgcaagc agcaaacagc aaacagtcga ctggacacag gacgctgtgg 360
tttctgagcc agggggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc 420
cgataactgg ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga 480
tccactgctt aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac 540
tgacctggga cagtgaatcg cgatcgccac catgcagatt gagctgagca cctgcttctt 600
cctgtgcctg ctgaggttct gcttctctgc caccaggaga tactacctgg gggctgtgga 660
gctgagctgg gactacatgc agtctgacct gggggagctg cctgtggatg ccaggttccc 720
ccccagagtg cccaagagct tccccttcaa cacctctgtg gtgtacaaga agaccctgtt 780
tgtggagttc actgaccacc tgttcaacat tgccaagccc aggcccccct ggatgggcct 840
gctgggcccc accatccagg ctgaggtgta tgacactgtg gtgatcaccc tgaagaacat 900
ggccagccac cctgtgagcc tgcatgctgt gggggtgagc tactggaagg cctctgaggg 960
ggctgagtat gatgaccaga ccagccagag ggagaaggag gatgacaagg tgttccctgg 1020
gggcagccac acctatgtgt ggcaggtgct gaaggagaat ggccccatgg cctctgaccc 1080
cctgtgcctg acctacagct acctgagcca tgtggacctg gtgaaggacc tgaactctgg 1140
cctgattggg gccctgctgg tgtgcaggga gggcagcctg gccaaggaga agacccagac 1200
cctgcacaag ttcatcctgc tgtttgctgt gtttgatgag ggcaagagct ggcactctga 1260
aaccaagaac agcctgatgc aggacaggga tgctgcctct gccagggcct ggcccaagat 1320
gcacactgtg aatggctatg tgaacaggag cctgcctggc ctgattggct gccacaggaa 1380
gtctgtgtac tggcatgtga ttggcatggg caccacccct gaggtgcaca gcatcttcct 1440
ggagggccac accttcctgg tcaggaacca caggcaggcc agcctggaga tcagccccat 1500
caccttcctg actgcccaga ccctgctgat ggacctgggc cagttcctgc tgttctgcca 1560
catcagcagc caccagcatg atggcatgga ggcctatgtg aaggtggaca gctgccctga 1620
ggagccccag ctgaggatga agaacaatga ggaggctgag gactatgatg atgacctgac 1680
tgactctgag atggatgtgg tgaggtttga tgatgacaac agccccagct tcatccagat 1740
caggtctgtg gccaagaagc accccaagac ctgggtgcac tacattgctg ctgaggagga 1800
ggactgggac tatgcccccc tggtgctggc ccctgatgac aggagctaca agagccagta 1860
cctgaacaat ggcccccaga ggattggcag gaagtacaag aaggtcaggt tcatggccta 1920
cactgatgaa accttcaaga ccagggaggc catccagcat gagtctggca tcctgggccc 1980
cctgctgtat ggggaggtgg gggacaccct gctgatcatc ttcaagaacc aggccagcag 2040
gccctacaac atctaccccc atggcatcac tgatgtgagg cccctgtaca gcaggaggct 2100
gcccaagggg gtgaagcacc tgaaggactt ccccatcctg cctggggaga tcttcaagta 2160
caagtggact gtgactgtgg aggatggccc caccaagtct gaccccaggt gcctgaccag 2220
atactacagc agctttgtga acatggagag ggacctggcc tctggcctga ttggccccct 2280
gctgatctgc tacaaggagt ctgtggacca gaggggcaac cagatcatgt ctgacaagag 2340
gaatgtgatc ctgttctctg tgtttgatga gaacaggagc tggtacctga ctgagaacat 2400
ccagaggttc ctgcccaacc ctgctggggt gcagctggag gaccctgagt tccaggccag 2460
caacatcatg cacagcatca atggctatgt gtttgacagc ctgcagctgt ctgtgtgcct 2520
gcatgaggtg gcctactggt acatcctgag cattggggcc cagactgact tcctgtctgt 2580
gttcttctct ggctacacct tcaagcacaa gatggtgtat gaggacaccc tgaccctgtt 2640
ccccttctct ggggagactg tgttcatgag catggagaac cctggcctgt ggattctggg 2700
ctgccacaac tctgacttca ggaacagggg catgactgcc ctgctgaaag tctccagctg 2760
tgacaagaac actggggact actatgagga cagctatgag gacatctctg cctacctgct 2820
gagcaagaac aatgccattg agcccaggag cttcagccag aaccccccag tgctgaagag 2880
gcaccagagg gagatcacca ggaccaccct gcagtctgac caggaggaga ttgactatga 2940
tgacaccatc tctgtggaga tgaagaagga ggactttgac atctacgacg aggacgagaa 3000
ccagagcccc aggagcttcc agaagaagac caggcactac ttcattgctg ctgtggagag 3060
gctgtgggac tatggcatga gcagcagccc ccatgtgctg aggaacaggg cccagtctgg 3120
ctctgtgccc cagttcaaga aggtggtgtt ccaggagttc actgatggca gcttcaccca 3180
gcccctgtac agaggggagc tgaatgagca cctgggcctg ctgggcccct acatcagggc 3240
tgaggtggag gacaacatca tggtgacctt caggaaccag gccagcaggc cctacagctt 3300
ctacagcagc ctgatcagct atgaggagga ccagaggcag ggggctgagc ccaggaagaa 3360
ctttgtgaag cccaatgaaa ccaagaccta cttctggaag gtgcagcacc acatggcccc 3420
caccaaggat gagtttgact gcaaggcctg ggcctacttc tctgatgtgg acctggagaa 3480
ggatgtgcac tctggcctga ttggccccct gctggtgtgc cacaccaaca ccctgaaccc 3540
tgcccatggc aggcaggtga ctgtgcagga gtttgccctg ttcttcacca tctttgatga 3600
aaccaagagc tggtacttca ctgagaacat ggagaggaac tgcagggccc cctgcaacat 3660
ccagatggag gaccccacct tcaaggagaa ctacaggttc catgccatca atggctacat 3720
catggacacc ctgcctggcc tggtgatggc ccaggaccag aggatcaggt ggtacctgct 3780
gagcatgggc agcaatgaga acatccacag catccacttc tctggccatg tgttcactgt 3840
gaggaagaag gaggagtaca agatggccct gtacaacctg taccctgggg tgtttgagac 3900
tgtggagatg ctgcccagca aggctggcat ctggagggtg gagtgcctga ttggggagca 3960
cctgcatgct ggcatgagca ccctgttcct ggtgtacagc aacaagtgcc agacccccct 4020
gggcatggcc tctggccaca tcagggactt ccagatcact gcctctggcc agtatggcca 4080
gtgggccccc aagctggcca ggctgcacta ctctggcagc atcaatgcct ggagcaccaa 4140
ggagcccttc agctggatca aggtggacct gctggccccc atgatcatcc atggcatcaa 4200
gacccagggg gccaggcaga agttcagcag cctgtacatc agccagttca tcatcatgta 4260
cagcctggat ggcaagaagt ggcagaccta caggggcaac agcactggca ccctgatggt 4320
gttctttggc aatgtggaca gctctggcat caagcacaac atcttcaacc cccccatcat 4380
tgccagatac atcaggctgc accccaccca ctacagcatc aggagcaccc tgaggatgga 4440
gctgatgggc tgtgacctga acagctgcag catgcccctg ggcatggaga gcaaggccat 4500
ctctgatgcc cagatcactg ccagcagcta cttcaccaac atgtttgcca cctggagccc 4560
cagcaaggcc aggctgcacc tgcagggcag gagcaatgcc tggaggcccc aggtcaacaa 4620
ccccaaggag tggctgcagg tggacttcca gaagaccatg aaggtgactg gggtgaccac 4680
ccagggggtg aagagcctgc tgaccagcat gtatgtgaag gagttcctga tcagcagcag 4740
ccaggatggc caccagtgga ccctgttctt ccagaatggc aaggtgaagg tgttccaggg 4800
caaccaggac agcttcaccc ctgtggtgaa cagcctggac ccccccctgc tgaccagata 4860
cctgaggatt cacccccaga gctgggtgca ccagattgcc ctgaggatgg aggtgctggg 4920
ctgtgaggcc caggacctgt actgacctcg aggaataaag gaaatttatt ttcattgcaa 4980
tagtgtgttg gttttttgtg tcacgtggcg gccgcaggaa cccctagtga tggagttggc 5040
cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg 5100
cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 5160
<210> 37
<211> 5383
<212> DNA
<213> 腺相关病毒2
<400> 37
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgact 360
ggacacagga cgctgtggtt tctgagccag ggggcgactc agatcccagc cagtggactt 420
agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc 480
ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc 540
agcttcaggc accaccactg acctgggaca gtgaatcgta agtatgcctt tcactgcgag 600
aggttctgga gaggcttctg agctccccat ggcccaggca ggcagcaggt ctggggcagg 660
aggggggttg tggagtgcct tgactcgggg cctggccccc ccatctctgt cttgcaggac 720
aattgccgtc ttctgtctcg tggggcatcc tcctgctggc aggcctgtgc tgcctggtcc 780
ctgcgatcgc caccatgcag attgagctga gcacctgctt cttcctgtgc ctgctgaggt 840
tctgcttctc tgccaccagg agatactacc tgggggctgt ggagctgagc tgggactaca 900
tgcagtctga cctgggggag ctgcctgtgg atgccaggtt cccccccaga gtgcccaaga 960
gcttcccctt caacacctct gtggtgtaca agaagaccct gtttgtggag ttcactgacc 1020
acctgttcaa cattgccaag cccaggcccc cctggatggg cctgctgggc cccaccatcc 1080
aggctgaggt gtatgacact gtggtgatca ccctgaagaa catggccagc caccctgtga 1140
gcctgcatgc tgtgggggtg agctactgga aggcctctga gggggctgag tatgatgacc 1200
agaccagcca gagggagaag gaggatgaca aggtgttccc tgggggcagc cacacctatg 1260
tgtggcaggt gctgaaggag aatggcccca tggcctctga ccccctgtgc ctgacctaca 1320
gctacctgag ccatgtggac ctggtgaagg acctgaactc tggcctgatt ggggccctgc 1380
tggtgtgcag ggagggcagc ctggccaagg agaagaccca gaccctgcac aagttcatcc 1440
tgctgtttgc tgtgtttgat gagggcaaga gctggcactc tgaaaccaag aacagcctga 1500
tgcaggacag ggatgctgcc tctgccaggg cctggcccaa gatgcacact gtgaatggct 1560
atgtgaacag gagcctgcct ggcctgattg gctgccacag gaagtctgtg tactggcatg 1620
tgattggcat gggcaccacc cctgaggtgc acagcatctt cctggagggc cacaccttcc 1680
tggtcaggaa ccacaggcag gccagcctgg agatcagccc catcaccttc ctgactgccc 1740
agaccctgct gatggacctg ggccagttcc tgctgttctg ccacatcagc agccaccagc 1800
atgatggcat ggaggcctat gtgaaggtgg acagctgccc tgaggagccc cagctgagga 1860
tgaagaacaa tgaggaggct gaggactatg atgatgacct gactgactct gagatggatg 1920
tggtgaggtt tgatgatgac aacagcccca gcttcatcca gatcaggtct gtggccaaga 1980
agcaccccaa gacctgggtg cactacattg ctgctgagga ggaggactgg gactatgccc 2040
ccctggtgct ggcccctgat gacaggagct acaagagcca gtacctgaac aatggccccc 2100
agaggattgg caggaagtac aagaaggtca ggttcatggc ctacactgat gaaaccttca 2160
agaccaggga ggccatccag catgagtctg gcatcctggg ccccctgctg tatggggagg 2220
tgggggacac cctgctgatc atcttcaaga accaggccag caggccctac aacatctacc 2280
cccatggcat cactgatgtg aggcccctgt acagcaggag gctgcccaag ggggtgaagc 2340
acctgaagga cttccccatc ctgcctgggg agatcttcaa gtacaagtgg actgtgactg 2400
tggaggatgg ccccaccaag tctgacccca ggtgcctgac cagatactac agcagctttg 2460
tgaacatgga gagggacctg gcctctggcc tgattggccc cctgctgatc tgctacaagg 2520
agtctgtgga ccagaggggc aaccagatca tgtctgacaa gaggaatgtg atcctgttct 2580
ctgtgtttga tgagaacagg agctggtacc tgactgagaa catccagagg ttcctgccca 2640
accctgctgg ggtgcagctg gaggaccctg agttccaggc cagcaacatc atgcacagca 2700
tcaatggcta tgtgtttgac agcctgcagc tgtctgtgtg cctgcatgag gtggcctact 2760
ggtacatcct gagcattggg gcccagactg acttcctgtc tgtgttcttc tctggctaca 2820
ccttcaagca caagatggtg tatgaggaca ccctgaccct gttccccttc tctggggaga 2880
ctgtgttcat gagcatggag aaccctggcc tgtggattct gggctgccac aactctgact 2940
tcaggaacag gggcatgact gccctgctga aagtctccag ctgtgacaag aacactgggg 3000
actactatga ggacagctat gaggacatct ctgcctacct gctgagcaag aacaatgcca 3060
ttgagcccag gagcttcagc cagaaccccc cagtgctgaa gaggcaccag agggagatca 3120
ccaggaccac cctgcagtct gaccaggagg agattgacta tgatgacacc atctctgtgg 3180
agatgaagaa ggaggacttt gacatctacg acgaggacga gaaccagagc cccaggagct 3240
tccagaagaa gaccaggcac tacttcattg ctgctgtgga gaggctgtgg gactatggca 3300
tgagcagcag cccccatgtg ctgaggaaca gggcccagtc tggctctgtg ccccagttca 3360
agaaggtggt gttccaggag ttcactgatg gcagcttcac ccagcccctg tacagagggg 3420
agctgaatga gcacctgggc ctgctgggcc cctacatcag ggctgaggtg gaggacaaca 3480
tcatggtgac cttcaggaac caggccagca ggccctacag cttctacagc agcctgatca 3540
gctatgagga ggaccagagg cagggggctg agcccaggaa gaactttgtg aagcccaatg 3600
aaaccaagac ctacttctgg aaggtgcagc accacatggc ccccaccaag gatgagtttg 3660
actgcaaggc ctgggcctac ttctctgatg tggacctgga gaaggatgtg cactctggcc 3720
tgattggccc cctgctggtg tgccacacca acaccctgaa ccctgcccat ggcaggcagg 3780
tgactgtgca ggagtttgcc ctgttcttca ccatctttga tgaaaccaag agctggtact 3840
tcactgagaa catggagagg aactgcaggg ccccctgcaa catccagatg gaggacccca 3900
ccttcaagga gaactacagg ttccatgcca tcaatggcta catcatggac accctgcctg 3960
gcctggtgat ggcccaggac cagaggatca ggtggtacct gctgagcatg ggcagcaatg 4020
agaacatcca cagcatccac ttctctggcc atgtgttcac tgtgaggaag aaggaggagt 4080
acaagatggc cctgtacaac ctgtaccctg gggtgtttga gactgtggag atgctgccca 4140
gcaaggctgg catctggagg gtggagtgcc tgattgggga gcacctgcat gctggcatga 4200
gcaccctgtt cctggtgtac agcaacaagt gccagacccc cctgggcatg gcctctggcc 4260
acatcaggga cttccagatc actgcctctg gccagtatgg ccagtgggcc cccaagctgg 4320
ccaggctgca ctactctggc agcatcaatg cctggagcac caaggagccc ttcagctgga 4380
tcaaggtgga cctgctggcc cccatgatca tccatggcat caagacccag ggggccaggc 4440
agaagttcag cagcctgtac atcagccagt tcatcatcat gtacagcctg gatggcaaga 4500
agtggcagac ctacaggggc aacagcactg gcaccctgat ggtgttcttt ggcaatgtgg 4560
acagctctgg catcaagcac aacatcttca acccccccat cattgccaga tacatcaggc 4620
tgcaccccac ccactacagc atcaggagca ccctgaggat ggagctgatg ggctgtgacc 4680
tgaacagctg cagcatgccc ctgggcatgg agagcaaggc catctctgat gcccagatca 4740
ctgccagcag ctacttcacc aacatgtttg ccacctggag ccccagcaag gccaggctgc 4800
acctgcaggg caggagcaat gcctggaggc cccaggtcaa caaccccaag gagtggctgc 4860
aggtggactt ccagaagacc atgaaggtga ctggggtgac cacccagggg gtgaagagcc 4920
tgctgaccag catgtatgtg aaggagttcc tgatcagcag cagccaggat ggccaccagt 4980
ggaccctgtt cttccagaat ggcaaggtga aggtgttcca gggcaaccag gacagcttca 5040
cccctgtggt gaacagcctg gacccccccc tgctgaccag atacctgagg attcaccccc 5100
agagctgggt gcaccagatt gccctgagga tggaggtgct gggctgtgag gcccaggacc 5160
tgtactgacc tcgaggaata aaggaaattt attttcattg caatagtgtg ttggtttttt 5220
gtgtcacgtg gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg 5280
ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg 5340
cggcctcagt gagcgagcga gcgcgcagag agggagtggc caa 5383
<210> 38
<211> 5728
<212> DNA
<213> 腺相关病毒2
<400> 38
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgaca 360
ggttaatttt taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct 420
gtttgctctg gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca 480
acatcctgga cttatcctct gggcctctcc ccacccccag gagaggctca ggttaatttt 540
taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct gtttgctctg 600
gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca acatcctgga 660
cttatcctct gggcctctcc ccacccccag gagaggctgt cgactggaca caggacgctg 720
tggtttctga gccagggggc gactcagatc ccagccagtg gacttagccc ctgtttgctc 780
ctccgataac tggggtgacc ttggttaata ttcaccagca gcctcccccg ttgcccctct 840
ggatccactg cttaaatacg gacgaggaca gggccctgtc tcctcagctt caggcaccac 900
cactgacctg ggacagtgaa tcgtaagtat gcctttcact gcgagaggtt ctggagaggc 960
ttctgagctc cccatggccc aggcaggcag caggtctggg gcaggagggg ggttgtggag 1020
tgccttgact cggggcctgg cccccccatc tctgtcttgc aggacaattg ccgtcttctg 1080
tctcgtgggg catcctcctg ctggcaggcc tgtgctgcct ggtccctgcg atcgccacca 1140
tgcagattga gctgagcacc tgcttcttcc tgtgcctgct gaggttctgc ttctctgcca 1200
ccaggagata ctacctgggg gctgtggagc tgagctggga ctacatgcag tctgacctgg 1260
gggagctgcc tgtggatgcc aggttccccc ccagagtgcc caagagcttc cccttcaaca 1320
cctctgtggt gtacaagaag accctgtttg tggagttcac tgaccacctg ttcaacattg 1380
ccaagcccag gcccccctgg atgggcctgc tgggccccac catccaggct gaggtgtatg 1440
acactgtggt gatcaccctg aagaacatgg ccagccaccc tgtgagcctg catgctgtgg 1500
gggtgagcta ctggaaggcc tctgaggggg ctgagtatga tgaccagacc agccagaggg 1560
agaaggagga tgacaaggtg ttccctgggg gcagccacac ctatgtgtgg caggtgctga 1620
aggagaatgg ccccatggcc tctgaccccc tgtgcctgac ctacagctac ctgagccatg 1680
tggacctggt gaaggacctg aactctggcc tgattggggc cctgctggtg tgcagggagg 1740
gcagcctggc caaggagaag acccagaccc tgcacaagtt catcctgctg tttgctgtgt 1800
ttgatgaggg caagagctgg cactctgaaa ccaagaacag cctgatgcag gacagggatg 1860
ctgcctctgc cagggcctgg cccaagatgc acactgtgaa tggctatgtg aacaggagcc 1920
tgcctggcct gattggctgc cacaggaagt ctgtgtactg gcatgtgatt ggcatgggca 1980
ccacccctga ggtgcacagc atcttcctgg agggccacac cttcctggtc aggaaccaca 2040
ggcaggccag cctggagatc agccccatca ccttcctgac tgcccagacc ctgctgatgg 2100
acctgggcca gttcctgctg ttctgccaca tcagcagcca ccagcatgat ggcatggagg 2160
cctatgtgaa ggtggacagc tgccctgagg agccccagct gaggatgaag aacaatgagg 2220
aggctgagga ctatgatgat gacctgactg actctgagat ggatgtggtg aggtttgatg 2280
atgacaacag ccccagcttc atccagatca ggtctgtggc caagaagcac cccaagacct 2340
gggtgcacta cattgctgct gaggaggagg actgggacta tgcccccctg gtgctggccc 2400
ctgatgacag gagctacaag agccagtacc tgaacaatgg cccccagagg attggcagga 2460
agtacaagaa ggtcaggttc atggcctaca ctgatgaaac cttcaagacc agggaggcca 2520
tccagcatga gtctggcatc ctgggccccc tgctgtatgg ggaggtgggg gacaccctgc 2580
tgatcatctt caagaaccag gccagcaggc cctacaacat ctacccccat ggcatcactg 2640
atgtgaggcc cctgtacagc aggaggctgc ccaagggggt gaagcacctg aaggacttcc 2700
ccatcctgcc tggggagatc ttcaagtaca agtggactgt gactgtggag gatggcccca 2760
ccaagtctga ccccaggtgc ctgaccagat actacagcag ctttgtgaac atggagaggg 2820
acctggcctc tggcctgatt ggccccctgc tgatctgcta caaggagtct gtggaccaga 2880
ggggcaacca gatcatgtct gacaagagga atgtgatcct gttctctgtg tttgatgaga 2940
acaggagctg gtacctgact gagaacatcc agaggttcct gcccaaccct gctggggtgc 3000
agctggagga ccctgagttc caggccagca acatcatgca cagcatcaat ggctatgtgt 3060
ttgacagcct gcagctgtct gtgtgcctgc atgaggtggc ctactggtac atcctgagca 3120
ttggggccca gactgacttc ctgtctgtgt tcttctctgg ctacaccttc aagcacaaga 3180
tggtgtatga ggacaccctg accctgttcc ccttctctgg ggagactgtg ttcatgagca 3240
tggagaaccc tggcctgtgg attctgggct gccacaactc tgacttcagg aacaggggca 3300
tgactgccct gctgaaagtc tccagctgtg acaagaacac tggggactac tatgaggaca 3360
gctatgagga catctctgcc tacctgctga gcaagaacaa tgccattgag cccaggagct 3420
tcagccagaa ccccccagtg ctgaagaggc accagaggga gatcaccagg accaccctgc 3480
agtctgacca ggaggagatt gactatgatg acaccatctc tgtggagatg aagaaggagg 3540
actttgacat ctacgacgag gacgagaacc agagccccag gagcttccag aagaagacca 3600
ggcactactt cattgctgct gtggagaggc tgtgggacta tggcatgagc agcagccccc 3660
atgtgctgag gaacagggcc cagtctggct ctgtgcccca gttcaagaag gtggtgttcc 3720
aggagttcac tgatggcagc ttcacccagc ccctgtacag aggggagctg aatgagcacc 3780
tgggcctgct gggcccctac atcagggctg aggtggagga caacatcatg gtgaccttca 3840
ggaaccaggc cagcaggccc tacagcttct acagcagcct gatcagctat gaggaggacc 3900
agaggcaggg ggctgagccc aggaagaact ttgtgaagcc caatgaaacc aagacctact 3960
tctggaaggt gcagcaccac atggccccca ccaaggatga gtttgactgc aaggcctggg 4020
cctacttctc tgatgtggac ctggagaagg atgtgcactc tggcctgatt ggccccctgc 4080
tggtgtgcca caccaacacc ctgaaccctg cccatggcag gcaggtgact gtgcaggagt 4140
ttgccctgtt cttcaccatc tttgatgaaa ccaagagctg gtacttcact gagaacatgg 4200
agaggaactg cagggccccc tgcaacatcc agatggagga ccccaccttc aaggagaact 4260
acaggttcca tgccatcaat ggctacatca tggacaccct gcctggcctg gtgatggccc 4320
aggaccagag gatcaggtgg tacctgctga gcatgggcag caatgagaac atccacagca 4380
tccacttctc tggccatgtg ttcactgtga ggaagaagga ggagtacaag atggccctgt 4440
acaacctgta ccctggggtg tttgagactg tggagatgct gcccagcaag gctggcatct 4500
ggagggtgga gtgcctgatt ggggagcacc tgcatgctgg catgagcacc ctgttcctgg 4560
tgtacagcaa caagtgccag acccccctgg gcatggcctc tggccacatc agggacttcc 4620
agatcactgc ctctggccag tatggccagt gggcccccaa gctggccagg ctgcactact 4680
ctggcagcat caatgcctgg agcaccaagg agcccttcag ctggatcaag gtggacctgc 4740
tggcccccat gatcatccat ggcatcaaga cccagggggc caggcagaag ttcagcagcc 4800
tgtacatcag ccagttcatc atcatgtaca gcctggatgg caagaagtgg cagacctaca 4860
ggggcaacag cactggcacc ctgatggtgt tctttggcaa tgtggacagc tctggcatca 4920
agcacaacat cttcaacccc cccatcattg ccagatacat caggctgcac cccacccact 4980
acagcatcag gagcaccctg aggatggagc tgatgggctg tgacctgaac agctgcagca 5040
tgcccctggg catggagagc aaggccatct ctgatgccca gatcactgcc agcagctact 5100
tcaccaacat gtttgccacc tggagcccca gcaaggccag gctgcacctg cagggcagga 5160
gcaatgcctg gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga 5220
agaccatgaa ggtgactggg gtgaccaccc agggggtgaa gagcctgctg accagcatgt 5280
atgtgaagga gttcctgatc agcagcagcc aggatggcca ccagtggacc ctgttcttcc 5340
agaatggcaa ggtgaaggtg ttccagggca accaggacag cttcacccct gtggtgaaca 5400
gcctggaccc ccccctgctg accagatacc tgaggattca cccccagagc tgggtgcacc 5460
agattgccct gaggatggag gtgctgggct gtgaggccca ggacctgtac tgacctcgag 5520
gaataaagga aatttatttt cattgcaata gtgtgttggt tttttgtgtc acgtggcggc 5580
cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg 5640
aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg 5700
agcgagcgcg cagagaggga gtggccaa 5728
<210> 39
<211> 5905
<212> DNA
<213> 腺相关病毒2
<400> 39
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgaca 360
ggttaatttt taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct 420
gtttgctctg gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca 480
acatcctgga cttatcctct gggcctctcc ccacccccag gagaggctca ggttaatttt 540
taaaaagcag tcaaaagtcc aagtggccct tggcagcatt tactctctct gtttgctctg 600
gttaataatc tcaggagcac aaacattcct ggaggcagga gaagaaatca acatcctgga 660
cttatcctct gggcctctcc ccacccccag gagaggctgt cgactggaca caggacgctg 720
tggtttctga gccagggggc gactcagatc ccagccagtg gacttagccc ctgtttgctc 780
ctccgataac tggggtgacc ttggttaata ttcaccagca gcctcccccg ttgcccctct 840
ggatccactg cttaaatacg gacgaggaca gggccctgtc tcctcagctt caggcaccac 900
cactgacctg ggacagtgaa tcgtaagtat gcctttcact gcgagaggtt ctggagaggc 960
ttctgagctc cccatggccc aggcaggcag caggtctggg gcaggagggg ggttgtggag 1020
tgccttgact cggggcctgg cccccccatc tctgtcttgc aggacaattg ccgtcttctg 1080
tctcgtgggg catcctcctg ctggcaggcc tgtgctgcct ggtccctgcg atcgccacca 1140
tgcagattga gctgagcacc tgcttcttcc tgtgcctgct gaggttctgc ttctctgcca 1200
ccaggagata ctacctgggg gctgtggagc tgagctggga ctacatgcag tctgacctgg 1260
gggagctgcc tgtggatgcc aggttccccc ccagagtgcc caagagcttc cccttcaaca 1320
cctctgtggt gtacaagaag accctgtttg tggagttcac tgaccacctg ttcaacattg 1380
ccaagcccag gcccccctgg atgggcctgc tgggccccac catccaggct gaggtgtatg 1440
acactgtggt gatcaccctg aagaacatgg ccagccaccc tgtgagcctg catgctgtgg 1500
gggtgagcta ctggaaggcc tctgaggggg ctgagtatga tgaccagacc agccagaggg 1560
agaaggagga tgacaaggtg ttccctgggg gcagccacac ctatgtgtgg caggtgctga 1620
aggagaatgg ccccatggcc tctgaccccc tgtgcctgac ctacagctac ctgagccatg 1680
tggacctggt gaaggacctg aactctggcc tgattggggc cctgctggtg tgcagggagg 1740
gcagcctggc caaggagaag acccagaccc tgcacaagtt catcctgctg tttgctgtgt 1800
ttgatgaggg caagagctgg cactctgaaa ccaagaacag cctgatgcag gacagggatg 1860
ctgcctctgc cagggcctgg cccaagatgc acactgtgaa tggctatgtg aacaggagcc 1920
tgcctggcct gattggctgc cacaggaagt ctgtgtactg gcatgtgatt ggcatgggca 1980
ccacccctga ggtgcacagc atcttcctgg agggccacac cttcctggtc aggaaccaca 2040
ggcaggccag cctggagatc agccccatca ccttcctgac tgcccagacc ctgctgatgg 2100
acctgggcca gttcctgctg ttctgccaca tcagcagcca ccagcatgat ggcatggagg 2160
cctatgtgaa ggtggacagc tgccctgagg agccccagct gaggatgaag aacaatgagg 2220
aggctgagga ctatgatgat gacctgactg actctgagat ggatgtggtg aggtttgatg 2280
atgacaacag ccccagcttc atccagatca ggtctgtggc caagaagcac cccaagacct 2340
gggtgcacta cattgctgct gaggaggagg actgggacta tgcccccctg gtgctggccc 2400
ctgatgacag gagctacaag agccagtacc tgaacaatgg cccccagagg attggcagga 2460
agtacaagaa ggtcaggttc atggcctaca ctgatgaaac cttcaagacc agggaggcca 2520
tccagcatga gtctggcatc ctgggccccc tgctgtatgg ggaggtgggg gacaccctgc 2580
tgatcatctt caagaaccag gccagcaggc cctacaacat ctacccccat ggcatcactg 2640
atgtgaggcc cctgtacagc aggaggctgc ccaagggggt gaagcacctg aaggacttcc 2700
ccatcctgcc tggggagatc ttcaagtaca agtggactgt gactgtggag gatggcccca 2760
ccaagtctga ccccaggtgc ctgaccagat actacagcag ctttgtgaac atggagaggg 2820
acctggcctc tggcctgatt ggccccctgc tgatctgcta caaggagtct gtggaccaga 2880
ggggcaacca gatcatgtct gacaagagga atgtgatcct gttctctgtg tttgatgaga 2940
acaggagctg gtacctgact gagaacatcc agaggttcct gcccaaccct gctggggtgc 3000
agctggagga ccctgagttc caggccagca acatcatgca cagcatcaat ggctatgtgt 3060
ttgacagcct gcagctgtct gtgtgcctgc atgaggtggc ctactggtac atcctgagca 3120
ttggggccca gactgacttc ctgtctgtgt tcttctctgg ctacaccttc aagcacaaga 3180
tggtgtatga ggacaccctg accctgttcc ccttctctgg ggagactgtg ttcatgagca 3240
tggagaaccc tggcctgtgg attctgggct gccacaactc tgacttcagg aacaggggca 3300
tgactgccct gctgaaagtc tccagctgtg acaagaacac tggggactac tatgaggaca 3360
gctatgagga catctctgcc tacctgctga gcaagaacaa tgccattgag cccaggagct 3420
tcagccagaa ccccccagtg ctgaagaggc accagaggga gatcaccagg accaccctgc 3480
agtctgacca ggaggagatt gactatgatg acaccatctc tgtggagatg aagaaggagg 3540
actttgacat ctacgacgag gacgagaacc agagccccag gagcttccag aagaagacca 3600
ggcactactt cattgctgct gtggagaggc tgtgggacta tggcatgagc agcagccccc 3660
atgtgctgag gaacagggcc cagtctggct ctgtgcccca gttcaagaag gtggtgttcc 3720
aggagttcac tgatggcagc ttcacccagc ccctgtacag aggggagctg aatgagcacc 3780
tgggcctgct gggcccctac atcagggctg aggtggagga caacatcatg gtgaccttca 3840
ggaaccaggc cagcaggccc tacagcttct acagcagcct gatcagctat gaggaggacc 3900
agaggcaggg ggctgagccc aggaagaact ttgtgaagcc caatgaaacc aagacctact 3960
tctggaaggt gcagcaccac atggccccca ccaaggatga gtttgactgc aaggcctggg 4020
cctacttctc tgatgtggac ctggagaagg atgtgcactc tggcctgatt ggccccctgc 4080
tggtgtgcca caccaacacc ctgaaccctg cccatggcag gcaggtgact gtgcaggagt 4140
ttgccctgtt cttcaccatc tttgatgaaa ccaagagctg gtacttcact gagaacatgg 4200
agaggaactg cagggccccc tgcaacatcc agatggagga ccccaccttc aaggagaact 4260
acaggttcca tgccatcaat ggctacatca tggacaccct gcctggcctg gtgatggccc 4320
aggaccagag gatcaggtgg tacctgctga gcatgggcag caatgagaac atccacagca 4380
tccacttctc tggccatgtg ttcactgtga ggaagaagga ggagtacaag atggccctgt 4440
acaacctgta ccctggggtg tttgagactg tggagatgct gcccagcaag gctggcatct 4500
ggagggtgga gtgcctgatt ggggagcacc tgcatgctgg catgagcacc ctgttcctgg 4560
tgtacagcaa caagtgccag acccccctgg gcatggcctc tggccacatc agggacttcc 4620
agatcactgc ctctggccag tatggccagt gggcccccaa gctggccagg ctgcactact 4680
ctggcagcat caatgcctgg agcaccaagg agcccttcag ctggatcaag gtggacctgc 4740
tggcccccat gatcatccat ggcatcaaga cccagggggc caggcagaag ttcagcagcc 4800
tgtacatcag ccagttcatc atcatgtaca gcctggatgg caagaagtgg cagacctaca 4860
ggggcaacag cactggcacc ctgatggtgt tctttggcaa tgtggacagc tctggcatca 4920
agcacaacat cttcaacccc cccatcattg ccagatacat caggctgcac cccacccact 4980
acagcatcag gagcaccctg aggatggagc tgatgggctg tgacctgaac agctgcagca 5040
tgcccctggg catggagagc aaggccatct ctgatgccca gatcactgcc agcagctact 5100
tcaccaacat gtttgccacc tggagcccca gcaaggccag gctgcacctg cagggcagga 5160
gcaatgcctg gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga 5220
agaccatgaa ggtgactggg gtgaccaccc agggggtgaa gagcctgctg accagcatgt 5280
atgtgaagga gttcctgatc agcagcagcc aggatggcca ccagtggacc ctgttcttcc 5340
agaatggcaa ggtgaaggtg ttccagggca accaggacag cttcacccct gtggtgaaca 5400
gcctggaccc ccccctgctg accagatacc tgaggattca cccccagagc tgggtgcacc 5460
agattgccct gaggatggag gtgctgggct gtgaggccca ggacctgtac tgacctcgag 5520
gtgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc 5580
tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc 5640
tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt 5700
gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgggcacg tggcggccgc 5760
aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg 5820
ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc 5880
gagcgcgcag agagggagtg gccaa 5905
<210> 40
<211> 5355
<212> DNA
<213> 腺相关病毒2
<400> 40
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgactg tttgctgttt 180
gctgcttgca atgtttgccc attttaggga catgtttgct gtttgctgct tgcaatgttt 240
gcccatttta gggacatgtt tgctgtttgc tgcttgcaat gtttgcccat tttagggaca 300
tgtttgctgt ttgctgcttg caatgtttgc ccattttagg gacaacgcga aacgtcgact 360
ggacacagga cgctgtggtt tctgagccag ggggcgactc agatcccagc cagtggactt 420
agcccctgtt tgctcctccg ataactgggg tgaccttggt taatattcac cagcagcctc 480
ccccgttgcc cctctggatc cactgcttaa atacggacga ggacagggcc ctgtctcctc 540
agcttcaggc accaccactg acctgggaca gtgaatcgcg atcgccacca tgcagattga 600
gctgagcacc tgcttcttcc tgtgcctgct gaggttctgc ttctctgcca ccaggagata 660
ctacctgggg gctgtggagc tgagctggga ctacatgcag tctgacctgg gggagctgcc 720
tgtggatgcc aggttccccc ccagagtgcc caagagcttc cccttcaaca cctctgtggt 780
gtacaagaag accctgtttg tggagttcac tgaccacctg ttcaacattg ccaagcccag 840
gcccccctgg atgggcctgc tgggccccac catccaggct gaggtgtatg acactgtggt 900
gatcaccctg aagaacatgg ccagccaccc tgtgagcctg catgctgtgg gggtgagcta 960
ctggaaggcc tctgaggggg ctgagtatga tgaccagacc agccagaggg agaaggagga 1020
tgacaaggtg ttccctgggg gcagccacac ctatgtgtgg caggtgctga aggagaatgg 1080
ccccatggcc tctgaccccc tgtgcctgac ctacagctac ctgagccatg tggacctggt 1140
gaaggacctg aactctggcc tgattggggc cctgctggtg tgcagggagg gcagcctggc 1200
caaggagaag acccagaccc tgcacaagtt catcctgctg tttgctgtgt ttgatgaggg 1260
caagagctgg cactctgaaa ccaagaacag cctgatgcag gacagggatg ctgcctctgc 1320
cagggcctgg cccaagatgc acactgtgaa tggctatgtg aacaggagcc tgcctggcct 1380
gattggctgc cacaggaagt ctgtgtactg gcatgtgatt ggcatgggca ccacccctga 1440
ggtgcacagc atcttcctgg agggccacac cttcctggtc aggaaccaca ggcaggccag 1500
cctggagatc agccccatca ccttcctgac tgcccagacc ctgctgatgg acctgggcca 1560
gttcctgctg ttctgccaca tcagcagcca ccagcatgat ggcatggagg cctatgtgaa 1620
ggtggacagc tgccctgagg agccccagct gaggatgaag aacaatgagg aggctgagga 1680
ctatgatgat gacctgactg actctgagat ggatgtggtg aggtttgatg atgacaacag 1740
ccccagcttc atccagatca ggtctgtggc caagaagcac cccaagacct gggtgcacta 1800
cattgctgct gaggaggagg actgggacta tgcccccctg gtgctggccc ctgatgacag 1860
gagctacaag agccagtacc tgaacaatgg cccccagagg attggcagga agtacaagaa 1920
ggtcaggttc atggcctaca ctgatgaaac cttcaagacc agggaggcca tccagcatga 1980
gtctggcatc ctgggccccc tgctgtatgg ggaggtgggg gacaccctgc tgatcatctt 2040
caagaaccag gccagcaggc cctacaacat ctacccccat ggcatcactg atgtgaggcc 2100
cctgtacagc aggaggctgc ccaagggggt gaagcacctg aaggacttcc ccatcctgcc 2160
tggggagatc ttcaagtaca agtggactgt gactgtggag gatggcccca ccaagtctga 2220
ccccaggtgc ctgaccagat actacagcag ctttgtgaac atggagaggg acctggcctc 2280
tggcctgatt ggccccctgc tgatctgcta caaggagtct gtggaccaga ggggcaacca 2340
gatcatgtct gacaagagga atgtgatcct gttctctgtg tttgatgaga acaggagctg 2400
gtacctgact gagaacatcc agaggttcct gcccaaccct gctggggtgc agctggagga 2460
ccctgagttc caggccagca acatcatgca cagcatcaat ggctatgtgt ttgacagcct 2520
gcagctgtct gtgtgcctgc atgaggtggc ctactggtac atcctgagca ttggggccca 2580
gactgacttc ctgtctgtgt tcttctctgg ctacaccttc aagcacaaga tggtgtatga 2640
ggacaccctg accctgttcc ccttctctgg ggagactgtg ttcatgagca tggagaaccc 2700
tggcctgtgg attctgggct gccacaactc tgacttcagg aacaggggca tgactgccct 2760
gctgaaagtc tccagctgtg acaagaacac tggggactac tatgaggaca gctatgagga 2820
catctctgcc tacctgctga gcaagaacaa tgccattgag cccaggagct tcagccagaa 2880
ccccccagtg ctgaagaggc accagaggga gatcaccagg accaccctgc agtctgacca 2940
ggaggagatt gactatgatg acaccatctc tgtggagatg aagaaggagg actttgacat 3000
ctacgacgag gacgagaacc agagccccag gagcttccag aagaagacca ggcactactt 3060
cattgctgct gtggagaggc tgtgggacta tggcatgagc agcagccccc atgtgctgag 3120
gaacagggcc cagtctggct ctgtgcccca gttcaagaag gtggtgttcc aggagttcac 3180
tgatggcagc ttcacccagc ccctgtacag aggggagctg aatgagcacc tgggcctgct 3240
gggcccctac atcagggctg aggtggagga caacatcatg gtgaccttca ggaaccaggc 3300
cagcaggccc tacagcttct acagcagcct gatcagctat gaggaggacc agaggcaggg 3360
ggctgagccc aggaagaact ttgtgaagcc caatgaaacc aagacctact tctggaaggt 3420
gcagcaccac atggccccca ccaaggatga gtttgactgc aaggcctggg cctacttctc 3480
tgatgtggac ctggagaagg atgtgcactc tggcctgatt ggccccctgc tggtgtgcca 3540
caccaacacc ctgaaccctg cccatggcag gcaggtgact gtgcaggagt ttgccctgtt 3600
cttcaccatc tttgatgaaa ccaagagctg gtacttcact gagaacatgg agaggaactg 3660
cagggccccc tgcaacatcc agatggagga ccccaccttc aaggagaact acaggttcca 3720
tgccatcaat ggctacatca tggacaccct gcctggcctg gtgatggccc aggaccagag 3780
gatcaggtgg tacctgctga gcatgggcag caatgagaac atccacagca tccacttctc 3840
tggccatgtg ttcactgtga ggaagaagga ggagtacaag atggccctgt acaacctgta 3900
ccctggggtg tttgagactg tggagatgct gcccagcaag gctggcatct ggagggtgga 3960
gtgcctgatt ggggagcacc tgcatgctgg catgagcacc ctgttcctgg tgtacagcaa 4020
caagtgccag acccccctgg gcatggcctc tggccacatc agggacttcc agatcactgc 4080
ctctggccag tatggccagt gggcccccaa gctggccagg ctgcactact ctggcagcat 4140
caatgcctgg agcaccaagg agcccttcag ctggatcaag gtggacctgc tggcccccat 4200
gatcatccat ggcatcaaga cccagggggc caggcagaag ttcagcagcc tgtacatcag 4260
ccagttcatc atcatgtaca gcctggatgg caagaagtgg cagacctaca ggggcaacag 4320
cactggcacc ctgatggtgt tctttggcaa tgtggacagc tctggcatca agcacaacat 4380
cttcaacccc cccatcattg ccagatacat caggctgcac cccacccact acagcatcag 4440
gagcaccctg aggatggagc tgatgggctg tgacctgaac agctgcagca tgcccctggg 4500
catggagagc aaggccatct ctgatgccca gatcactgcc agcagctact tcaccaacat 4560
gtttgccacc tggagcccca gcaaggccag gctgcacctg cagggcagga gcaatgcctg 4620
gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg gacttccaga agaccatgaa 4680
ggtgactggg gtgaccaccc agggggtgaa gagcctgctg accagcatgt atgtgaagga 4740
gttcctgatc agcagcagcc aggatggcca ccagtggacc ctgttcttcc agaatggcaa 4800
ggtgaaggtg ttccagggca accaggacag cttcacccct gtggtgaaca gcctggaccc 4860
ccccctgctg accagatacc tgaggattca cccccagagc tgggtgcacc agattgccct 4920
gaggatggag gtgctgggct gtgaggccca ggacctgtac tgacctcgag gtgtgccttc 4980
tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 5040
cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 5100
tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 5160
tagcaggcat gctggggatg cggtgggctc tatgggcacg tggcggccgc aggaacccct 5220
agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc 5280
aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag 5340
agagggagtg gccaa 5355
<210> 41
<211> 5618
<212> DNA
<213> 腺相关病毒2
<400> 41
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ttttcgacca gagaggtctc 180
tgacctctgc cccagctcca aggtcagcag gcagggaggg ctgtgtgttt gctgtttgct 240
gcttgcaatg tttgcccatt ttagggacat gagtaggctg aagtttgttc agtgtggact 300
tcagaggcag cacacaaaca gccagagagg tctctgacct ctgccccagc tccaaggtca 360
gcaggcaggg agggctgtgt gtttgctgtt tgctgcttgc aatgtttgcc cattttaggg 420
acatgagtag gctgaagttt gttcagtgtg gacttcagag gcagcacaca aacagccaga 480
gaggtctctg acctctgccc cagctccaag gtcagcaggc agggagggct gtgtgtttgc 540
tgtttgctgc ttgcaatgtt tgcccatttt agggacatga gtaggctgaa gtttgttcag 600
tgtggacttc agaggcagca cacaaacagc cagagaggtc tctgacctct gccccagctc 660
caaggtcagc aggcagggag ggctgtgtgt ttgctgtttg ctgcttgcaa tgtttgccca 720
ttttagggac atgagtaggc tgaagtttgt tcagtgtgga cttcagaggc agcacacaaa 780
cagcacgcga aacgtcgact ggacacagga cgctgtggtt tctgagccag ggggcgactc 840
agatcccagc cagtggactt agcccctgtt tgctcctccg ataactgggg tgaccttggt 900
taatattcac cagcagcctc ccccgttgcc cctctggatc cactgcttaa atacggacga 960
ggacagggcc ctgtctcctc agcttcaggc accaccactg acctgggaca gtgaatcgcg 1020
atcgccacca tgcagattga gctgagcacc tgcttcttcc tgtgcctgct gaggttctgc 1080
ttctctgcca ccaggagata ctacctgggg gctgtggagc tgagctggga ctacatgcag 1140
tctgacctgg gggagctgcc tgtggatgcc aggttccccc ccagagtgcc caagagcttc 1200
cccttcaaca cctctgtggt gtacaagaag accctgtttg tggagttcac tgaccacctg 1260
ttcaacattg ccaagcccag gcccccctgg atgggcctgc tgggccccac catccaggct 1320
gaggtgtatg acactgtggt gatcaccctg aagaacatgg ccagccaccc tgtgagcctg 1380
catgctgtgg gggtgagcta ctggaaggcc tctgaggggg ctgagtatga tgaccagacc 1440
agccagaggg agaaggagga tgacaaggtg ttccctgggg gcagccacac ctatgtgtgg 1500
caggtgctga aggagaatgg ccccatggcc tctgaccccc tgtgcctgac ctacagctac 1560
ctgagccatg tggacctggt gaaggacctg aactctggcc tgattggggc cctgctggtg 1620
tgcagggagg gcagcctggc caaggagaag acccagaccc tgcacaagtt catcctgctg 1680
tttgctgtgt ttgatgaggg caagagctgg cactctgaaa ccaagaacag cctgatgcag 1740
gacagggatg ctgcctctgc cagggcctgg cccaagatgc acactgtgaa tggctatgtg 1800
aacaggagcc tgcctggcct gattggctgc cacaggaagt ctgtgtactg gcatgtgatt 1860
ggcatgggca ccacccctga ggtgcacagc atcttcctgg agggccacac cttcctggtc 1920
aggaaccaca ggcaggccag cctggagatc agccccatca ccttcctgac tgcccagacc 1980
ctgctgatgg acctgggcca gttcctgctg ttctgccaca tcagcagcca ccagcatgat 2040
ggcatggagg cctatgtgaa ggtggacagc tgccctgagg agccccagct gaggatgaag 2100
aacaatgagg aggctgagga ctatgatgat gacctgactg actctgagat ggatgtggtg 2160
aggtttgatg atgacaacag ccccagcttc atccagatca ggtctgtggc caagaagcac 2220
cccaagacct gggtgcacta cattgctgct gaggaggagg actgggacta tgcccccctg 2280
gtgctggccc ctgatgacag gagctacaag agccagtacc tgaacaatgg cccccagagg 2340
attggcagga agtacaagaa ggtcaggttc atggcctaca ctgatgaaac cttcaagacc 2400
agggaggcca tccagcatga gtctggcatc ctgggccccc tgctgtatgg ggaggtgggg 2460
gacaccctgc tgatcatctt caagaaccag gccagcaggc cctacaacat ctacccccat 2520
ggcatcactg atgtgaggcc cctgtacagc aggaggctgc ccaagggggt gaagcacctg 2580
aaggacttcc ccatcctgcc tggggagatc ttcaagtaca agtggactgt gactgtggag 2640
gatggcccca ccaagtctga ccccaggtgc ctgaccagat actacagcag ctttgtgaac 2700
atggagaggg acctggcctc tggcctgatt ggccccctgc tgatctgcta caaggagtct 2760
gtggaccaga ggggcaacca gatcatgtct gacaagagga atgtgatcct gttctctgtg 2820
tttgatgaga acaggagctg gtacctgact gagaacatcc agaggttcct gcccaaccct 2880
gctggggtgc agctggagga ccctgagttc caggccagca acatcatgca cagcatcaat 2940
ggctatgtgt ttgacagcct gcagctgtct gtgtgcctgc atgaggtggc ctactggtac 3000
atcctgagca ttggggccca gactgacttc ctgtctgtgt tcttctctgg ctacaccttc 3060
aagcacaaga tggtgtatga ggacaccctg accctgttcc ccttctctgg ggagactgtg 3120
ttcatgagca tggagaaccc tggcctgtgg attctgggct gccacaactc tgacttcagg 3180
aacaggggca tgactgccct gctgaaagtc tccagctgtg acaagaacac tggggactac 3240
tatgaggaca gctatgagga catctctgcc tacctgctga gcaagaacaa tgccattgag 3300
cccaggagct tcagccagaa ccccccagtg ctgaagaggc accagaggga gatcaccagg 3360
accaccctgc agtctgacca ggaggagatt gactatgatg acaccatctc tgtggagatg 3420
aagaaggagg actttgacat ctacgacgag gacgagaacc agagccccag gagcttccag 3480
aagaagacca ggcactactt cattgctgct gtggagaggc tgtgggacta tggcatgagc 3540
agcagccccc atgtgctgag gaacagggcc cagtctggct ctgtgcccca gttcaagaag 3600
gtggtgttcc aggagttcac tgatggcagc ttcacccagc ccctgtacag aggggagctg 3660
aatgagcacc tgggcctgct gggcccctac atcagggctg aggtggagga caacatcatg 3720
gtgaccttca ggaaccaggc cagcaggccc tacagcttct acagcagcct gatcagctat 3780
gaggaggacc agaggcaggg ggctgagccc aggaagaact ttgtgaagcc caatgaaacc 3840
aagacctact tctggaaggt gcagcaccac atggccccca ccaaggatga gtttgactgc 3900
aaggcctggg cctacttctc tgatgtggac ctggagaagg atgtgcactc tggcctgatt 3960
ggccccctgc tggtgtgcca caccaacacc ctgaaccctg cccatggcag gcaggtgact 4020
gtgcaggagt ttgccctgtt cttcaccatc tttgatgaaa ccaagagctg gtacttcact 4080
gagaacatgg agaggaactg cagggccccc tgcaacatcc agatggagga ccccaccttc 4140
aaggagaact acaggttcca tgccatcaat ggctacatca tggacaccct gcctggcctg 4200
gtgatggccc aggaccagag gatcaggtgg tacctgctga gcatgggcag caatgagaac 4260
atccacagca tccacttctc tggccatgtg ttcactgtga ggaagaagga ggagtacaag 4320
atggccctgt acaacctgta ccctggggtg tttgagactg tggagatgct gcccagcaag 4380
gctggcatct ggagggtgga gtgcctgatt ggggagcacc tgcatgctgg catgagcacc 4440
ctgttcctgg tgtacagcaa caagtgccag acccccctgg gcatggcctc tggccacatc 4500
agggacttcc agatcactgc ctctggccag tatggccagt gggcccccaa gctggccagg 4560
ctgcactact ctggcagcat caatgcctgg agcaccaagg agcccttcag ctggatcaag 4620
gtggacctgc tggcccccat gatcatccat ggcatcaaga cccagggggc caggcagaag 4680
ttcagcagcc tgtacatcag ccagttcatc atcatgtaca gcctggatgg caagaagtgg 4740
cagacctaca ggggcaacag cactggcacc ctgatggtgt tctttggcaa tgtggacagc 4800
tctggcatca agcacaacat cttcaacccc cccatcattg ccagatacat caggctgcac 4860
cccacccact acagcatcag gagcaccctg aggatggagc tgatgggctg tgacctgaac 4920
agctgcagca tgcccctggg catggagagc aaggccatct ctgatgccca gatcactgcc 4980
agcagctact tcaccaacat gtttgccacc tggagcccca gcaaggccag gctgcacctg 5040
cagggcagga gcaatgcctg gaggccccag gtcaacaacc ccaaggagtg gctgcaggtg 5100
gacttccaga agaccatgaa ggtgactggg gtgaccaccc agggggtgaa gagcctgctg 5160
accagcatgt atgtgaagga gttcctgatc agcagcagcc aggatggcca ccagtggacc 5220
ctgttcttcc agaatggcaa ggtgaaggtg ttccagggca accaggacag cttcacccct 5280
gtggtgaaca gcctggaccc ccccctgctg accagatacc tgaggattca cccccagagc 5340
tgggtgcacc agattgccct gaggatggag gtgctgggct gtgaggccca ggacctgtac 5400
tgacctcgag gaataaagga aatttatttt cattgcaata gtgtgttggt tttttgtgtc 5460
acgtggcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc 5520
tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc 5580
tcagtgagcg agcgagcgcg cagagaggga gtggccaa 5618
<210> 42
<211> 5993
<212> DNA
<213> 腺相关病毒2
<400> 42
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tttaaacgtc gacaggttaa 180
tttttaaaaa gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc 240
tctggttaat aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc 300
tggacttatc ctctgggcct ctccccaccc ccaggagagg ctcaggttaa tttttaaaaa 360
gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc tctggttaat 420
aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc tggacttatc 480
ctctgggcct ctccccaccc ccaggagagg ctgtcgactg gacacaggac gctgtggttt 540
ctgagccagg gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga 600
taactggggt gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc 660
actgcttaaa tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga 720
cctgggacag tgaatcgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct 780
gtgcctgctg aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct 840
gagctgggac tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc 900
cagagtgccc aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt 960
ggagttcact gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct 1020
gggccccacc atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc 1080
cagccaccct gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc 1140
tgagtatgat gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg 1200
cagccacacc tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct 1260
gtgcctgacc tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct 1320
gattggggcc ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct 1380
gcacaagttc atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac 1440
caagaacagc ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca 1500
cactgtgaat ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc 1560
tgtgtactgg catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga 1620
gggccacacc ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac 1680
cttcctgact gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat 1740
cagcagccac cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga 1800
gccccagctg aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga 1860
ctctgagatg gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag 1920
gtctgtggcc aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga 1980
ctgggactat gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct 2040
gaacaatggc ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac 2100
tgatgaaacc ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct 2160
gctgtatggg gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc 2220
ctacaacatc tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc 2280
caagggggtg aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa 2340
gtggactgtg actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata 2400
ctacagcagc tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct 2460
gatctgctac aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa 2520
tgtgatcctg ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca 2580
gaggttcctg cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa 2640
catcatgcac agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca 2700
tgaggtggcc tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt 2760
cttctctggc tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc 2820
cttctctggg gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg 2880
ccacaactct gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga 2940
caagaacact ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag 3000
caagaacaat gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca 3060
ccagagggag atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga 3120
caccatctct gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca 3180
gagccccagg agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct 3240
gtgggactat ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc 3300
tgtgccccag ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc 3360
cctgtacaga ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga 3420
ggtggaggac aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta 3480
cagcagcctg atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt 3540
tgtgaagccc aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac 3600
caaggatgag tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga 3660
tgtgcactct ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc 3720
ccatggcagg caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac 3780
caagagctgg tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca 3840
gatggaggac cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat 3900
ggacaccctg cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag 3960
catgggcagc aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag 4020
gaagaaggag gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt 4080
ggagatgctg cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct 4140
gcatgctggc atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg 4200
catggcctct ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg 4260
ggcccccaag ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga 4320
gcccttcagc tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac 4380
ccagggggcc aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag 4440
cctggatggc aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt 4500
ctttggcaat gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc 4560
cagatacatc aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct 4620
gatgggctgt gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc 4680
tgatgcccag atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag 4740
caaggccagg ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc 4800
caaggagtgg ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca 4860
gggggtgaag agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca 4920
ggatggccac cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa 4980
ccaggacagc ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct 5040
gaggattcac ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg 5100
tgaggcccag gacctgtact gacctcgagg aataaaggaa atttattttc attgcaatag 5160
tgtgttggtt ttttgtgtca cgtgccctct cacactacct aaaccacgcc aggacaacct 5220
ctgctcctct ccaccgaaat tccaaggggt cgagtggatg ttggaggtgg catgggccca 5280
gagaggtctc tgacctctgc cccagctcca aggtcagcag gcagggaggg ctgtgtgttt 5340
gctgtttgct gcttgcaatg tttgcccatt ttagggacat gagtaggctg aagtttgttc 5400
agtgtggact tcagaggcag cacacaaaca gctgctggag gatgggaact gaggggttgg 5460
aagggggcag ggtgagccca gaaactcctg tgtgcctctg agcctgcagc cctctcacac 5520
tacctaaacc acgccaggac aacctctgct cctctccacc gaaattccaa ggggtcgagt 5580
ggatgttgga ggtggcatgg gcccagagag gtctctgacc tctgccccag ctccaaggtc 5640
agcaggcagg gagggctgtg tgtttgctgt ttgctgcttg caatgtttgc ccattttagg 5700
gacatgagta ggctgaagtt tgttcagtgt ggacttcaga ggcagcacac aaacagctgc 5760
tggaggatgg gaactgaggg gttggaaggg ggcagggtga gcccagaaac tcctgtgtgc 5820
ctctgagcct gcagcacgtg gcggccgcag gaacccctag tgatggagtt ggccactccc 5880
tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 5940
tttgcccggg cggcctcagt gagcgagcga gcgcgcagag agggagtggc caa 5993
<210> 43
<211> 5337
<212> DNA
<213> 腺相关病毒2
<400> 43
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tttaaacgtc gacaggttaa 180
tttttaaaaa gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc 240
tctggttaat aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc 300
tggacttatc ctctgggcct ctccccaccc ccaggagagg ctcaggttaa tttttaaaaa 360
gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc tctggttaat 420
aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc tggacttatc 480
ctctgggcct ctccccaccc ccaggagagg ctgtcgactg gacacaggac gctgtggttt 540
ctgagccagg gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga 600
taactggggt gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc 660
actgcttaaa tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga 720
cctgggacag tgaatcgcga tcgccaccat gcagattgag ctgagcacct gcttcttcct 780
gtgcctgctg aggttctgct tctctgccac caggagatac tacctggggg ctgtggagct 840
gagctgggac tacatgcagt ctgacctggg ggagctgcct gtggatgcca ggttcccccc 900
cagagtgccc aagagcttcc ccttcaacac ctctgtggtg tacaagaaga ccctgtttgt 960
ggagttcact gaccacctgt tcaacattgc caagcccagg cccccctgga tgggcctgct 1020
gggccccacc atccaggctg aggtgtatga cactgtggtg atcaccctga agaacatggc 1080
cagccaccct gtgagcctgc atgctgtggg ggtgagctac tggaaggcct ctgagggggc 1140
tgagtatgat gaccagacca gccagaggga gaaggaggat gacaaggtgt tccctggggg 1200
cagccacacc tatgtgtggc aggtgctgaa ggagaatggc cccatggcct ctgaccccct 1260
gtgcctgacc tacagctacc tgagccatgt ggacctggtg aaggacctga actctggcct 1320
gattggggcc ctgctggtgt gcagggaggg cagcctggcc aaggagaaga cccagaccct 1380
gcacaagttc atcctgctgt ttgctgtgtt tgatgagggc aagagctggc actctgaaac 1440
caagaacagc ctgatgcagg acagggatgc tgcctctgcc agggcctggc ccaagatgca 1500
cactgtgaat ggctatgtga acaggagcct gcctggcctg attggctgcc acaggaagtc 1560
tgtgtactgg catgtgattg gcatgggcac cacccctgag gtgcacagca tcttcctgga 1620
gggccacacc ttcctggtca ggaaccacag gcaggccagc ctggagatca gccccatcac 1680
cttcctgact gcccagaccc tgctgatgga cctgggccag ttcctgctgt tctgccacat 1740
cagcagccac cagcatgatg gcatggaggc ctatgtgaag gtggacagct gccctgagga 1800
gccccagctg aggatgaaga acaatgagga ggctgaggac tatgatgatg acctgactga 1860
ctctgagatg gatgtggtga ggtttgatga tgacaacagc cccagcttca tccagatcag 1920
gtctgtggcc aagaagcacc ccaagacctg ggtgcactac attgctgctg aggaggagga 1980
ctgggactat gcccccctgg tgctggcccc tgatgacagg agctacaaga gccagtacct 2040
gaacaatggc ccccagagga ttggcaggaa gtacaagaag gtcaggttca tggcctacac 2100
tgatgaaacc ttcaagacca gggaggccat ccagcatgag tctggcatcc tgggccccct 2160
gctgtatggg gaggtggggg acaccctgct gatcatcttc aagaaccagg ccagcaggcc 2220
ctacaacatc tacccccatg gcatcactga tgtgaggccc ctgtacagca ggaggctgcc 2280
caagggggtg aagcacctga aggacttccc catcctgcct ggggagatct tcaagtacaa 2340
gtggactgtg actgtggagg atggccccac caagtctgac cccaggtgcc tgaccagata 2400
ctacagcagc tttgtgaaca tggagaggga cctggcctct ggcctgattg gccccctgct 2460
gatctgctac aaggagtctg tggaccagag gggcaaccag atcatgtctg acaagaggaa 2520
tgtgatcctg ttctctgtgt ttgatgagaa caggagctgg tacctgactg agaacatcca 2580
gaggttcctg cccaaccctg ctggggtgca gctggaggac cctgagttcc aggccagcaa 2640
catcatgcac agcatcaatg gctatgtgtt tgacagcctg cagctgtctg tgtgcctgca 2700
tgaggtggcc tactggtaca tcctgagcat tggggcccag actgacttcc tgtctgtgtt 2760
cttctctggc tacaccttca agcacaagat ggtgtatgag gacaccctga ccctgttccc 2820
cttctctggg gagactgtgt tcatgagcat ggagaaccct ggcctgtgga ttctgggctg 2880
ccacaactct gacttcagga acaggggcat gactgccctg ctgaaagtct ccagctgtga 2940
caagaacact ggggactact atgaggacag ctatgaggac atctctgcct acctgctgag 3000
caagaacaat gccattgagc ccaggagctt cagccagaac cccccagtgc tgaagaggca 3060
ccagagggag atcaccagga ccaccctgca gtctgaccag gaggagattg actatgatga 3120
caccatctct gtggagatga agaaggagga ctttgacatc tacgacgagg acgagaacca 3180
gagccccagg agcttccaga agaagaccag gcactacttc attgctgctg tggagaggct 3240
gtgggactat ggcatgagca gcagccccca tgtgctgagg aacagggccc agtctggctc 3300
tgtgccccag ttcaagaagg tggtgttcca ggagttcact gatggcagct tcacccagcc 3360
cctgtacaga ggggagctga atgagcacct gggcctgctg ggcccctaca tcagggctga 3420
ggtggaggac aacatcatgg tgaccttcag gaaccaggcc agcaggccct acagcttcta 3480
cagcagcctg atcagctatg aggaggacca gaggcagggg gctgagccca ggaagaactt 3540
tgtgaagccc aatgaaacca agacctactt ctggaaggtg cagcaccaca tggcccccac 3600
caaggatgag tttgactgca aggcctgggc ctacttctct gatgtggacc tggagaagga 3660
tgtgcactct ggcctgattg gccccctgct ggtgtgccac accaacaccc tgaaccctgc 3720
ccatggcagg caggtgactg tgcaggagtt tgccctgttc ttcaccatct ttgatgaaac 3780
caagagctgg tacttcactg agaacatgga gaggaactgc agggccccct gcaacatcca 3840
gatggaggac cccaccttca aggagaacta caggttccat gccatcaatg gctacatcat 3900
ggacaccctg cctggcctgg tgatggccca ggaccagagg atcaggtggt acctgctgag 3960
catgggcagc aatgagaaca tccacagcat ccacttctct ggccatgtgt tcactgtgag 4020
gaagaaggag gagtacaaga tggccctgta caacctgtac cctggggtgt ttgagactgt 4080
ggagatgctg cccagcaagg ctggcatctg gagggtggag tgcctgattg gggagcacct 4140
gcatgctggc atgagcaccc tgttcctggt gtacagcaac aagtgccaga cccccctggg 4200
catggcctct ggccacatca gggacttcca gatcactgcc tctggccagt atggccagtg 4260
ggcccccaag ctggccaggc tgcactactc tggcagcatc aatgcctgga gcaccaagga 4320
gcccttcagc tggatcaagg tggacctgct ggcccccatg atcatccatg gcatcaagac 4380
ccagggggcc aggcagaagt tcagcagcct gtacatcagc cagttcatca tcatgtacag 4440
cctggatggc aagaagtggc agacctacag gggcaacagc actggcaccc tgatggtgtt 4500
ctttggcaat gtggacagct ctggcatcaa gcacaacatc ttcaaccccc ccatcattgc 4560
cagatacatc aggctgcacc ccacccacta cagcatcagg agcaccctga ggatggagct 4620
gatgggctgt gacctgaaca gctgcagcat gcccctgggc atggagagca aggccatctc 4680
tgatgcccag atcactgcca gcagctactt caccaacatg tttgccacct ggagccccag 4740
caaggccagg ctgcacctgc agggcaggag caatgcctgg aggccccagg tcaacaaccc 4800
caaggagtgg ctgcaggtgg acttccagaa gaccatgaag gtgactgggg tgaccaccca 4860
gggggtgaag agcctgctga ccagcatgta tgtgaaggag ttcctgatca gcagcagcca 4920
ggatggccac cagtggaccc tgttcttcca gaatggcaag gtgaaggtgt tccagggcaa 4980
ccaggacagc ttcacccctg tggtgaacag cctggacccc cccctgctga ccagatacct 5040
gaggattcac ccccagagct gggtgcacca gattgccctg aggatggagg tgctgggctg 5100
tgaggcccag gacctgtact gacctcgagg aataaaggaa atttattttc attgcaatag 5160
tgtgttggtt ttttgtgtca cgtggcggcc gcaggaaccc ctagtgatgg agttggccac 5220
tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc 5280
gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agagagggag tggccaa 5337
<210> 44
<211> 5542
<212> DNA
<213> 腺相关病毒2
<400> 44
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg tttaaacgtc gacaggttaa 180
tttttaaaaa gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc 240
tctggttaat aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc 300
tggacttatc ctctgggcct ctccccaccc ccaggagagg ctcaggttaa tttttaaaaa 360
gcagtcaaaa gtccaagtgg cccttggcag catttactct ctctgtttgc tctggttaat 420
aatctcagga gcacaaacat tcctggaggc aggagaagaa atcaacatcc tggacttatc 480
ctctgggcct ctccccaccc ccaggagagg ctgtcgactg gacacaggac gctgtggttt 540
ctgagccagg gggcgactca gatcccagcc agtggactta gcccctgttt gctcctccga 600
taactggggt gaccttggtt aatattcacc agcagcctcc cccgttgccc ctctggatcc 660
actgcttaaa tacggacgag gacagggccc tgtctcctca gcttcaggca ccaccactga 720
cctgggacag tgaatcgtaa gtatgccttt cactgcgaga ggttctggag aggcttctga 780
gctccccatg gcccaggcag gcagcaggtc tggggcagga ggggggttgt ggagtgcctt 840
gactcggggc ctggcccccc catctctgtc ttgcaggaca attgccgtct tctgtctcgt 900
ggggcatcct cctgctggca ggcctgtgct gcctggtccc tgcgatcgcc accatgcaga 960
ttgagctgag cacctgcttc ttcctgtgcc tgctgaggtt ctgcttctct gccaccagga 1020
gatactacct gggggctgtg gagctgagct gggactacat gcagtctgac ctgggggagc 1080
tgcctgtgga tgccaggttc ccccccagag tgcccaagag cttccccttc aacacctctg 1140
tggtgtacaa gaagaccctg tttgtggagt tcactgacca cctgttcaac attgccaagc 1200
ccaggccccc ctggatgggc ctgctgggcc ccaccatcca ggctgaggtg tatgacactg 1260
tggtgatcac cctgaagaac atggccagcc accctgtgag cctgcatgct gtgggggtga 1320
gctactggaa ggcctctgag ggggctgagt atgatgacca gaccagccag agggagaagg 1380
aggatgacaa ggtgttccct gggggcagcc acacctatgt gtggcaggtg ctgaaggaga 1440
atggccccat ggcctctgac cccctgtgcc tgacctacag ctacctgagc catgtggacc 1500
tggtgaagga cctgaactct ggcctgattg gggccctgct ggtgtgcagg gagggcagcc 1560
tggccaagga gaagacccag accctgcaca agttcatcct gctgtttgct gtgtttgatg 1620
agggcaagag ctggcactct gaaaccaaga acagcctgat gcaggacagg gatgctgcct 1680
ctgccagggc ctggcccaag atgcacactg tgaatggcta tgtgaacagg agcctgcctg 1740
gcctgattgg ctgccacagg aagtctgtgt actggcatgt gattggcatg ggcaccaccc 1800
ctgaggtgca cagcatcttc ctggagggcc acaccttcct ggtcaggaac cacaggcagg 1860
ccagcctgga gatcagcccc atcaccttcc tgactgccca gaccctgctg atggacctgg 1920
gccagttcct gctgttctgc cacatcagca gccaccagca tgatggcatg gaggcctatg 1980
tgaaggtgga cagctgccct gaggagcccc agctgaggat gaagaacaat gaggaggctg 2040
aggactatga tgatgacctg actgactctg agatggatgt ggtgaggttt gatgatgaca 2100
acagccccag cttcatccag atcaggtctg tggccaagaa gcaccccaag acctgggtgc 2160
actacattgc tgctgaggag gaggactggg actatgcccc cctggtgctg gcccctgatg 2220
acaggagcta caagagccag tacctgaaca atggccccca gaggattggc aggaagtaca 2280
agaaggtcag gttcatggcc tacactgatg aaaccttcaa gaccagggag gccatccagc 2340
atgagtctgg catcctgggc cccctgctgt atggggaggt gggggacacc ctgctgatca 2400
tcttcaagaa ccaggccagc aggccctaca acatctaccc ccatggcatc actgatgtga 2460
ggcccctgta cagcaggagg ctgcccaagg gggtgaagca cctgaaggac ttccccatcc 2520
tgcctgggga gatcttcaag tacaagtgga ctgtgactgt ggaggatggc cccaccaagt 2580
ctgaccccag gtgcctgacc agatactaca gcagctttgt gaacatggag agggacctgg 2640
cctctggcct gattggcccc ctgctgatct gctacaagga gtctgtggac cagaggggca 2700
accagatcat gtctgacaag aggaatgtga tcctgttctc tgtgtttgat gagaacagga 2760
gctggtacct gactgagaac atccagaggt tcctgcccaa ccctgctggg gtgcagctgg 2820
aggaccctga gttccaggcc agcaacatca tgcacagcat caatggctat gtgtttgaca 2880
gcctgcagct gtctgtgtgc ctgcatgagg tggcctactg gtacatcctg agcattgggg 2940
cccagactga cttcctgtct gtgttcttct ctggctacac cttcaagcac aagatggtgt 3000
atgaggacac cctgaccctg ttccccttct ctggggagac tgtgttcatg agcatggaga 3060
accctggcct gtggattctg ggctgccaca actctgactt caggaacagg ggcatgactg 3120
ccctgctgaa agtctccagc tgtgacaaga acactgggga ctactatgag gacagctatg 3180
aggacatctc tgcctacctg ctgagcaaga acaatgccat tgagcccagg agcttcagcc 3240
agaacccccc agtgctgaag aggcaccaga gggagatcac caggaccacc ctgcagtctg 3300
accaggagga gattgactat gatgacacca tctctgtgga gatgaagaag gaggactttg 3360
acatctacga cgaggacgag aaccagagcc ccaggagctt ccagaagaag accaggcact 3420
acttcattgc tgctgtggag aggctgtggg actatggcat gagcagcagc ccccatgtgc 3480
tgaggaacag ggcccagtct ggctctgtgc cccagttcaa gaaggtggtg ttccaggagt 3540
tcactgatgg cagcttcacc cagcccctgt acagagggga gctgaatgag cacctgggcc 3600
tgctgggccc ctacatcagg gctgaggtgg aggacaacat catggtgacc ttcaggaacc 3660
aggccagcag gccctacagc ttctacagca gcctgatcag ctatgaggag gaccagaggc 3720
agggggctga gcccaggaag aactttgtga agcccaatga aaccaagacc tacttctgga 3780
aggtgcagca ccacatggcc cccaccaagg atgagtttga ctgcaaggcc tgggcctact 3840
tctctgatgt ggacctggag aaggatgtgc actctggcct gattggcccc ctgctggtgt 3900
gccacaccaa caccctgaac cctgcccatg gcaggcaggt gactgtgcag gagtttgccc 3960
tgttcttcac catctttgat gaaaccaaga gctggtactt cactgagaac atggagagga 4020
actgcagggc cccctgcaac atccagatgg aggaccccac cttcaaggag aactacaggt 4080
tccatgccat caatggctac atcatggaca ccctgcctgg cctggtgatg gcccaggacc 4140
agaggatcag gtggtacctg ctgagcatgg gcagcaatga gaacatccac agcatccact 4200
tctctggcca tgtgttcact gtgaggaaga aggaggagta caagatggcc ctgtacaacc 4260
tgtaccctgg ggtgtttgag actgtggaga tgctgcccag caaggctggc atctggaggg 4320
tggagtgcct gattggggag cacctgcatg ctggcatgag caccctgttc ctggtgtaca 4380
gcaacaagtg ccagaccccc ctgggcatgg cctctggcca catcagggac ttccagatca 4440
ctgcctctgg ccagtatggc cagtgggccc ccaagctggc caggctgcac tactctggca 4500
gcatcaatgc ctggagcacc aaggagccct tcagctggat caaggtggac ctgctggccc 4560
ccatgatcat ccatggcatc aagacccagg gggccaggca gaagttcagc agcctgtaca 4620
tcagccagtt catcatcatg tacagcctgg atggcaagaa gtggcagacc tacaggggca 4680
acagcactgg caccctgatg gtgttctttg gcaatgtgga cagctctggc atcaagcaca 4740
acatcttcaa cccccccatc attgccagat acatcaggct gcaccccacc cactacagca 4800
tcaggagcac cctgaggatg gagctgatgg gctgtgacct gaacagctgc agcatgcccc 4860
tgggcatgga gagcaaggcc atctctgatg cccagatcac tgccagcagc tacttcacca 4920
acatgtttgc cacctggagc cccagcaagg ccaggctgca cctgcagggc aggagcaatg 4980
cctggaggcc ccaggtcaac aaccccaagg agtggctgca ggtggacttc cagaagacca 5040
tgaaggtgac tggggtgacc acccaggggg tgaagagcct gctgaccagc atgtatgtga 5100
aggagttcct gatcagcagc agccaggatg gccaccagtg gaccctgttc ttccagaatg 5160
gcaaggtgaa ggtgttccag ggcaaccagg acagcttcac ccctgtggtg aacagcctgg 5220
acccccccct gctgaccaga tacctgagga ttcaccccca gagctgggtg caccagattg 5280
ccctgaggat ggaggtgctg ggctgtgagg cccaggacct gtactgacct cgaggaataa 5340
aggaaattta ttttcattgc aatagtgtgt tggttttttg tgtcacgtgg cggccgcagg 5400
aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg 5460
ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag 5520
cgcgcagaga gggagtggcc aa 5542
<210> 45
<211> 5126
<212> DNA
<213> 腺相关病毒2
<400> 45
ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg 60
cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg 120
gccaactcca tcactagggg ttcctgcggc cgcacgcgtg ggaggctgct ggtgaatatt 180
aaccaaggtc accccagtta tcggaggagc aaacaggggc taagtccacg gggaggctgc 240
tggtgaatat taaccaaggt caccccagtt atcggaggag caaacagggg ctaagtccac 300
ggtcgactgg acacaggacg ctgtggtttc tgagccaggg ggcgactcag atcccagcca 360
gtggacttag cccctgtttg ctcctccgat aactggggtg accttggtta atattcacca 420
gcagcctccc ccgttgcccc tctggatcca ctgcttaaat acggacgagg acagggccct 480
gtctcctcag cttcaggcac caccactgac ctgggacagt gaatcgcgat cgccaccatg 540
cagattgagc tgagcacctg cttcttcctg tgcctgctga ggttctgctt ctctgccacc 600
aggagatact acctgggggc tgtggagctg agctgggact acatgcagtc tgacctgggg 660
gagctgcctg tggatgccag gttccccccc agagtgccca agagcttccc cttcaacacc 720
tctgtggtgt acaagaagac cctgtttgtg gagttcactg accacctgtt caacattgcc 780
aagcccaggc ccccctggat gggcctgctg ggccccacca tccaggctga ggtgtatgac 840
actgtggtga tcaccctgaa gaacatggcc agccaccctg tgagcctgca tgctgtgggg 900
gtgagctact ggaaggcctc tgagggggct gagtatgatg accagaccag ccagagggag 960
aaggaggatg acaaggtgtt ccctgggggc agccacacct atgtgtggca ggtgctgaag 1020
gagaatggcc ccatggcctc tgaccccctg tgcctgacct acagctacct gagccatgtg 1080
gacctggtga aggacctgaa ctctggcctg attggggccc tgctggtgtg cagggagggc 1140
agcctggcca aggagaagac ccagaccctg cacaagttca tcctgctgtt tgctgtgttt 1200
gatgagggca agagctggca ctctgaaacc aagaacagcc tgatgcagga cagggatgct 1260
gcctctgcca gggcctggcc caagatgcac actgtgaatg gctatgtgaa caggagcctg 1320
cctggcctga ttggctgcca caggaagtct gtgtactggc atgtgattgg catgggcacc 1380
acccctgagg tgcacagcat cttcctggag ggccacacct tcctggtcag gaaccacagg 1440
caggccagcc tggagatcag ccccatcacc ttcctgactg cccagaccct gctgatggac 1500
ctgggccagt tcctgctgtt ctgccacatc agcagccacc agcatgatgg catggaggcc 1560
tatgtgaagg tggacagctg ccctgaggag ccccagctga ggatgaagaa caatgaggag 1620
gctgaggact atgatgatga cctgactgac tctgagatgg atgtggtgag gtttgatgat 1680
gacaacagcc ccagcttcat ccagatcagg tctgtggcca agaagcaccc caagacctgg 1740
gtgcactaca ttgctgctga ggaggaggac tgggactatg cccccctggt gctggcccct 1800
gatgacagga gctacaagag ccagtacctg aacaatggcc cccagaggat tggcaggaag 1860
tacaagaagg tcaggttcat ggcctacact gatgaaacct tcaagaccag ggaggccatc 1920
cagcatgagt ctggcatcct gggccccctg ctgtatgggg aggtggggga caccctgctg 1980
atcatcttca agaaccaggc cagcaggccc tacaacatct acccccatgg catcactgat 2040
gtgaggcccc tgtacagcag gaggctgccc aagggggtga agcacctgaa ggacttcccc 2100
atcctgcctg gggagatctt caagtacaag tggactgtga ctgtggagga tggccccacc 2160
aagtctgacc ccaggtgcct gaccagatac tacagcagct ttgtgaacat ggagagggac 2220
ctggcctctg gcctgattgg ccccctgctg atctgctaca aggagtctgt ggaccagagg 2280
ggcaaccaga tcatgtctga caagaggaat gtgatcctgt tctctgtgtt tgatgagaac 2340
aggagctggt acctgactga gaacatccag aggttcctgc ccaaccctgc tggggtgcag 2400
ctggaggacc ctgagttcca ggccagcaac atcatgcaca gcatcaatgg ctatgtgttt 2460
gacagcctgc agctgtctgt gtgcctgcat gaggtggcct actggtacat cctgagcatt 2520
ggggcccaga ctgacttcct gtctgtgttc ttctctggct acaccttcaa gcacaagatg 2580
gtgtatgagg acaccctgac cctgttcccc ttctctgggg agactgtgtt catgagcatg 2640
gagaaccctg gcctgtggat tctgggctgc cacaactctg acttcaggaa caggggcatg 2700
actgccctgc tgaaagtctc cagctgtgac aagaacactg gggactacta tgaggacagc 2760
tatgaggaca tctctgccta cctgctgagc aagaacaatg ccattgagcc caggagcttc 2820
agccagaacc ccccagtgct gaagaggcac cagagggaga tcaccaggac caccctgcag 2880
tctgaccagg aggagattga ctatgatgac accatctctg tggagatgaa gaaggaggac 2940
tttgacatct acgacgagga cgagaaccag agccccagga gcttccagaa gaagaccagg 3000
cactacttca ttgctgctgt ggagaggctg tgggactatg gcatgagcag cagcccccat 3060
gtgctgagga acagggccca gtctggctct gtgccccagt tcaagaaggt ggtgttccag 3120
gagttcactg atggcagctt cacccagccc ctgtacagag gggagctgaa tgagcacctg 3180
ggcctgctgg gcccctacat cagggctgag gtggaggaca acatcatggt gaccttcagg 3240
aaccaggcca gcaggcccta cagcttctac agcagcctga tcagctatga ggaggaccag 3300
aggcaggggg ctgagcccag gaagaacttt gtgaagccca atgaaaccaa gacctacttc 3360
tggaaggtgc agcaccacat ggcccccacc aaggatgagt ttgactgcaa ggcctgggcc 3420
tacttctctg atgtggacct ggagaaggat gtgcactctg gcctgattgg ccccctgctg 3480
gtgtgccaca ccaacaccct gaaccctgcc catggcaggc aggtgactgt gcaggagttt 3540
gccctgttct tcaccatctt tgatgaaacc aagagctggt acttcactga gaacatggag 3600
aggaactgca gggccccctg caacatccag atggaggacc ccaccttcaa ggagaactac 3660
aggttccatg ccatcaatgg ctacatcatg gacaccctgc ctggcctggt gatggcccag 3720
gaccagagga tcaggtggta cctgctgagc atgggcagca atgagaacat ccacagcatc 3780
cacttctctg gccatgtgtt cactgtgagg aagaaggagg agtacaagat ggccctgtac 3840
aacctgtacc ctggggtgtt tgagactgtg gagatgctgc ccagcaaggc tggcatctgg 3900
agggtggagt gcctgattgg ggagcacctg catgctggca tgagcaccct gttcctggtg 3960
tacagcaaca agtgccagac ccccctgggc atggcctctg gccacatcag ggacttccag 4020
atcactgcct ctggccagta tggccagtgg gcccccaagc tggccaggct gcactactct 4080
ggcagcatca atgcctggag caccaaggag cccttcagct ggatcaaggt ggacctgctg 4140
gcccccatga tcatccatgg catcaagacc cagggggcca ggcagaagtt cagcagcctg 4200
tacatcagcc agttcatcat catgtacagc ctggatggca agaagtggca gacctacagg 4260
ggcaacagca ctggcaccct gatggtgttc tttggcaatg tggacagctc tggcatcaag 4320
cacaacatct tcaacccccc catcattgcc agatacatca ggctgcaccc cacccactac 4380
agcatcagga gcaccctgag gatggagctg atgggctgtg acctgaacag ctgcagcatg 4440
cccctgggca tggagagcaa ggccatctct gatgcccaga tcactgccag cagctacttc 4500
accaacatgt ttgccacctg gagccccagc aaggccaggc tgcacctgca gggcaggagc 4560
aatgcctgga ggccccaggt caacaacccc aaggagtggc tgcaggtgga cttccagaag 4620
accatgaagg tgactggggt gaccacccag ggggtgaaga gcctgctgac cagcatgtat 4680
gtgaaggagt tcctgatcag cagcagccag gatggccacc agtggaccct gttcttccag 4740
aatggcaagg tgaaggtgtt ccagggcaac caggacagct tcacccctgt ggtgaacagc 4800
ctggaccccc ccctgctgac cagatacctg aggattcacc cccagagctg ggtgcaccag 4860
attgccctga ggatggaggt gctgggctgt gaggcccagg acctgtactg acctcgagga 4920
ataaaggaaa tttattttca ttgcaatagt gtgttggttt tttgtgtcac gtggcggccg 4980
caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag 5040
gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag 5100
cgagcgcgca gagagggagt ggccaa 5126

Claims (8)

1.一种腺相关病毒(AAV)因子VIII(FVIII)载体，所述载体包含核酸，所述核酸包含AAV2 5'反向末端重复序列(ITR)、肝脏特异性转录调节区、密码子优化的功能活性FVIII编码区、任选的一个或多个内含子、多聚腺苷酸化序列、以及AAV2 3'ITR。
2.如权利要求1所述的AAV FVIII载体，其中所述核酸包含选自由以下各项组成的组的核苷酸序列：
i.SEQ ID NO:1中所示的Proto 1序列；
ii.SEQ ID NO:2中所示的Proto 1S序列；
iii.SEQ ID NO:3中所示的Proto 2S序列；
iv.SEQ ID NO:4中所示的Proto 3S序列；
v.SEQ ID NO:5中所示的Proto 4序列；
vi.SEQ ID NO:6中所示的Proto 5序列；
vii.SEQ ID NO:7中所示的Proto 6序列；
viii.SEQ ID NO:8中所示的Proto 7序列；
ix.SEQ ID NO:9中所示的构建体100ATG序列；
x.SEQ ID NO:10中所示的构建体100ATG bGH多聚腺苷酸序列；
xi.SEQ ID NO:11中所示的构建体100ATG短bGH多聚腺苷酸序列；
xii.SEQ ID NO:12中所示的构建体103ATG序列；
xiii.SEQ ID NO:13中所示的构建体103ATG短bGH多聚腺苷酸序列；
xiv.SEQ ID NO:14中所示的构建体105ATG bGH多聚腺苷酸序列；
xv.SEQ ID NO:15中所示的构建体DC172ATG FVIII序列；
xvi.SEQ ID NO:16中所示的构建体DC172 ATG FVIII hAAT序列；
xvii.SEQ ID NO:17中所示的构建体DC172 2×HCR ATG FVIII序列；
xviii.SEQ ID NO:18中所示的构建体DC172 2×HCR ATG FVIII hAAT序列；
xix.SEQ ID NO:19中所示的构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII序列；
xx.SEQ ID NO:20中所示的构建体2×丝氨酸蛋白酶抑制蛋白A hAAT ATG FVIII 2×μ-球蛋白增强子序列；
xxi.SEQ ID NO:21中所示的构建体100ATG短多聚腺苷酸2×μ-球蛋白增强子序列；
xxii.SEQ ID NO:22中所示的构建体因子VIII-BMN001序列；
xxiii.SEQ ID NO:23中所示的构建体因子VIII-BMN002序列；
xxiv.SEQ ID NO:24中所示的构建体99序列；
xxv.SEQ ID NO:25中所示的构建体100序列；
xxvi.SEQ ID NO:26中所示的构建体100反向定向序列；
xxvii.SEQ ID NO:27中所示的构建体100AT序列；
xxviii.SEQ ID NO:28中所示的构建体100AT 2×MG序列；
xxix.SEQ ID NO:29中所示的构建体100AT 2×MG多聚腺苷酸序列；
xxx.SEQ ID NO:30中所示的构建体100AT 2×MG(反向)bGH多聚腺苷酸序列；
xxxi.SEQ ID NO:31中所示的构建体100bGH多聚腺苷酸序列；
xxxii.SEQ ID NO:32中所示的构建体100-400序列；
xxxiii.SEQ ID NO:33中所示的构建体101序列；
xxxiv.SEQ ID NO:34中所示的构建体102序列；
xxxv.SEQ ID NO:35中所示的构建体103序列；
xxxvi.SEQ ID NO:36中所示的构建体103反向定向序列；
xxxvii.SEQ ID NO:37中所示的构建体103AT序列；
xxxviii.SEQ ID NO:38中所示的构建体103AT 2×MG序列；
xxxix.SEQ ID NO:39中所示的构建体103AT 2×MG多聚腺苷酸序列；
xl.SEQ ID NO:40中所示的构建体103bGH多聚腺苷酸序列；
xli.SEQ ID NO:41中所示的构建体104序列；
xlii.SEQ ID NO:42中所示的构建体105序列；
xliii.SEQ ID NO:43中所示的构建体106序列；
xliv.SEQ ID NO:44中所示的构建体106AT序列；以及
xlv.SEQ ID NO:45中所示的构建体2×丝氨酸蛋白酶抑制蛋白A hAAT序列。
3.一种产生重组腺相关病毒(AAV)颗粒的方法，所述方法包括
A)培养已经被转染了如权利要求1或2所述的AAV载体的细胞；以及
B)从所述转染的细胞的上清液中回收重组AAV颗粒。
4.一种病毒颗粒，所述病毒颗粒包含如权利要求1或2所述的病毒载体。
5.一种细胞，所述细胞包含如权利要求1或2所述的病毒载体。
6.一种治疗患有A型血友病的患者的方法，所述方法包括向所述患者施用有效量的如权利要求1或2所述的AAV FVIII载体。
7.如权利要求1或2所述的AAV FVIII载体用于制备用于治疗A型血友病的药物的用途。
8.一种组合物，所述组合物包含如权利要求1或2所述的AAV FVIII载体以治疗A型血友病。
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