CN112522439B - 一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 - Google Patents
一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 Download PDFInfo
- Publication number
- CN112522439B CN112522439B CN202011260473.7A CN202011260473A CN112522439B CN 112522439 B CN112522439 B CN 112522439B CN 202011260473 A CN202011260473 A CN 202011260473A CN 112522439 B CN112522439 B CN 112522439B
- Authority
- CN
- China
- Prior art keywords
- hpv
- kit
- integration
- library
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000701806 Human papillomavirus Species 0.000 title claims abstract description 84
- 239000000523 sample Substances 0.000 title claims abstract description 79
- 230000010354 integration Effects 0.000 title claims abstract description 38
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 241000341655 Human papillomavirus type 16 Species 0.000 claims abstract description 8
- 238000013461 design Methods 0.000 claims description 8
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 38
- 206010008342 Cervix carcinoma Diseases 0.000 description 23
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 23
- 239000011324 bead Substances 0.000 description 23
- 201000010881 cervical cancer Diseases 0.000 description 23
- 238000006243 chemical reaction Methods 0.000 description 23
- 238000012163 sequencing technique Methods 0.000 description 21
- 238000003752 polymerase chain reaction Methods 0.000 description 20
- 239000003153 chemical reaction reagent Substances 0.000 description 18
- 239000007853 buffer solution Substances 0.000 description 16
- 238000000034 method Methods 0.000 description 16
- 238000002156 mixing Methods 0.000 description 16
- 238000009396 hybridization Methods 0.000 description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 15
- 238000012408 PCR amplification Methods 0.000 description 14
- 108090000623 proteins and genes Proteins 0.000 description 14
- 210000004027 cell Anatomy 0.000 description 13
- 239000012634 fragment Substances 0.000 description 13
- 239000007788 liquid Substances 0.000 description 13
- 230000000903 blocking effect Effects 0.000 description 12
- 239000000872 buffer Substances 0.000 description 11
- 239000011259 mixed solution Substances 0.000 description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000004140 cleaning Methods 0.000 description 8
- 238000012165 high-throughput sequencing Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 6
- 208000022361 Human papillomavirus infectious disease Diseases 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 230000008696 hypoxemic pulmonary vasoconstriction Effects 0.000 description 6
- 238000010257 thawing Methods 0.000 description 6
- 241000264288 mixed libraries Species 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 230000035945 sensitivity Effects 0.000 description 5
- 206010008263 Cervical dysplasia Diseases 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000007664 blowing Methods 0.000 description 4
- 230000004907 flux Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000003908 quality control method Methods 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 230000008439 repair process Effects 0.000 description 4
- 230000003612 virological effect Effects 0.000 description 4
- 208000009608 Papillomavirus Infections Diseases 0.000 description 3
- 238000011529 RT qPCR Methods 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 235000020958 biotin Nutrition 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 230000000875 corresponding effect Effects 0.000 description 3
- 239000013024 dilution buffer Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 230000036210 malignancy Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000012264 purified product Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- GUAHPAJOXVYFON-ZETCQYMHSA-N (8S)-8-amino-7-oxononanoic acid zwitterion Chemical compound C[C@H](N)C(=O)CCCCCC(O)=O GUAHPAJOXVYFON-ZETCQYMHSA-N 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 208000007951 cervical intraepithelial neoplasia Diseases 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000011541 reaction mixture Substances 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- 206010059313 Anogenital warts Diseases 0.000 description 1
- 101100343292 Arabidopsis thaliana LHT1 gene Proteins 0.000 description 1
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 208000009458 Carcinoma in Situ Diseases 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 101100384865 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cot-1 gene Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 208000037581 Persistent Infection Diseases 0.000 description 1
- 208000007313 Reproductive Tract Infections Diseases 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 238000010009 beating Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 210000003679 cervix uteri Anatomy 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002559 cytogenic effect Effects 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000013399 early diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 201000004933 in situ carcinoma Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000007481 next generation sequencing Methods 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 238000001921 nucleic acid quantification Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- XEBWQGVWTUSTLN-UHFFFAOYSA-M phenylmercury acetate Chemical compound CC(=O)O[Hg]C1=CC=CC=C1 XEBWQGVWTUSTLN-UHFFFAOYSA-M 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000008213 purified water Substances 0.000 description 1
- 238000001303 quality assessment method Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 206010041823 squamous cell carcinoma Diseases 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了一种用于检测5种人***瘤病毒HPV分型和整合的探针组及其试剂盒，所述探针组包含能特异性捕获以下5种HPV基因组的探针：HPV16、18、33、52、58；本发明检测范围全面，检测效率大幅提高且结果准确。
Description
技术领域：
本发明涉及一种探针组及其试剂盒，具体涉及一种可用于人***瘤病毒HPV基因捕获、测序的探针组及试剂盒。
背景技术：
***是指发生于子宫颈***和宫颈部的恶性肿瘤，是最常见的妇科恶性肿瘤之一，仅次于乳腺癌。***在中国15~44岁女性各癌症发病率中居第二位，死亡率居第三位。大量研究证实，***发生主要是由高危型人***瘤病毒（Human Papillomavirus, HPV）持续感染所致，在99.7%的***患者体内检测到高危型HPV DNA的存在。目前，HPV基因型已鉴定出200余种，约40种与生殖道感染相关。根据其导致***风险的高低将HPV分为高危型和低危险、型，其中高危型包括HPV16、18、31、33、35、39、45、51、52、56、58、59和68等13种基因型列为高危型，26、53、66、73、82等5种基因型列为中等风险型别。这18种型别的HPV主要与宫颈上皮内瘤变、CINⅡ、Ⅲ级和***的发生相关。低危型一般与恶性病变无关，主要引起***和低级别CIN（CINI）。因此，通过检测宫颈脱落细胞中是否有高危型HPV感染，并定期检测宫颈脱落细胞中HPV病毒是否持续性感染在***的早期筛查、辅助诊断和愈后评估等环节中显得尤为重要。
通常情况下，HPV感染寄主细胞之后以游离状态存在，其DNA仍自由存在寄主细胞的细胞质中，随寄主细胞的***复制，HPV DNA就会***人体基因组，这个过程也称为HPV整合。一旦HPV DNA偶然整合至宿主细胞染色体中，病毒蛋白将无秩序复制，宿主细胞遗传物质结构及相关基因表达发生变化，导致主体细胞不可控制地***，从而诱发癌变。尽管99.7%以上的***中均检测出HPV DNA，但却不是每一个感染高危型HPV的患者均发生了***。随着分子生物学和细胞遗传学的进展，HPV与宿主染色体的整合才是宫颈细胞永生化过程中的重要步骤，是导致宫颈上皮内瘤变向***恶性进展的一个重要标志。在90%以上的***组织中均发现HPV与宿主细胞染色体的整合体，HPV与宿主细胞基因组的整合是***发展的最重要的危险因素。低危型HPV很少导致***，与在宿主体内的病毒载量无关，是因为其很少发生病毒整合。高危型HPV分别在HSIL和鳞状细胞癌中的整合率分别为76.2%和88.2%，在LSIL中仅37%。HPV以非游离状态存在于侵入性***中（约60.9%），较原位癌高出20%。HPV16阳性***病例中近90%整合，纯游离形式存在的病例仅16%。说明病毒整合发生率与***发病率成正相关，病毒整合状态与***病情程度成正相关。HPV DNA与人基因组整合将作为宫颈病变程度的标志物，对***的早期诊断具有非常重要的意义。
***早期症状不明显，只有尽早地对***做出早期筛选预警，才能有效降低由***引起的死亡率。传统的病理学检测手段，如宫颈涂片检测，易受取材、染色及细胞病理医生的主观判断等因素的影响，因此应用细胞病理学检测HPV存在灵敏度低、特异性差、假阴性率和假阳性率高、不能对HPV进行分型等弊端。目前临床上常用的实时荧光聚合酶链反应(PCR)方法，该方法检测通量低，对于不同型别的HPV要分别检测，很难满足临床上同时进行多个型别的检测的需求，对于***的大规模筛查不太适合。更重要的是，目前所用的这些方法只能对有无HPV感染及HPV感染型别做出判断，对于HPV DNA是否与人基因组发生整合没法做出判断，而HPV整合事件是***发生的重要因素。高通量测序技术（next-generation sequencing technology）具有准确、灵敏度高、快速和低成本的优点，可同时对多个基因进行测序，该方法不只可以实现HPV分型检测，同时可以实现HPV整合检测。液相杂交基因捕获技术可以将目标区域相关基因片段富集，结合新一代基因测序技术，能够极大地降低人类基因测序、基因学研究及基因诊断的成本，提高测序深度，更精确地发现特定区域变异信息。而捕获探针的设计是液相杂交捕获技术的核心。综上所述，本发明提供一种用于检测HPV分型及整合的探针组及试剂盒，可一次性对18种高危型HPV进行精确分型并确定其在人基因组中的整合情况，具有一次性处理多个样本、灵敏度高和特异性高的优点，临床意义重大。
发明内容：
本发明的目的在于解决上述现有技术的不足，提供一种用于检测HPV分型及整合的捕获探针组及其试剂盒；本发明根据5种HPV全基因组序列设计合成探针序列，对目的片段进行特异性捕获、扩增和测序，以达到检测样本的目的。所述探针组包含能特异性捕获全部5种HPV基因组的探针。可以全面、快速、准确的检测5种HPV分型和整合情况，所述5种HPV为HPV16、18、33、52、58。
本发明采用以下技术方案：
本发明提供了一种用于检测5种人***瘤病毒HPV分型和整合的探针组，所述5种HPV为HPV16、18、33、52、58，所述探针组信见表1
本发明还提供了一种上述用于检测5种人***瘤病毒HPV分型和整合的试剂盒。所述试剂盒包括本发明所述的探针组；较佳的，所述试剂盒还包括建库缓冲液1、建库缓冲液2、酶1、酶2、接头、PCR扩增引物1、PCR扩增引物2、PCR反应混合液、封闭序列1、封闭序列2、封闭试剂、杂交缓冲液、PCR扩增缓冲液、PCR扩增引物3、PCR扩增反应液、阳性对照、阴性对照、捕获缓冲液、清洗缓冲液1、清洗缓冲液2和捕获磁珠；优选所述封闭序列1为Cot-1 DNA，封闭序列2为双链寡核苷酸。
进一步的，所述试剂盒各组成成分如表2所示。
本发明还公开了一种使用所述探针组及其试剂盒的方法，主要包括以下几个步骤：
(1)设计5种型别HPV的捕获探针
(2)样本DNA提取、片段化及构建DNA文库
(3)文库目标区域靶向捕获及定量
(4)高通量测序
(5)基因序列的生物信息分析
本发明提供一种对HPV基因进行捕获的方法，具体捕获过程包括：提取宫颈脱落细胞全基因组DNA，将其随机打断成200-300bp大小的片段后，部分片段含有HPV基因的序列，构建DNA文库；用本发明涉及的HPV探针（携带生物素标记，能特异性和HPVDNA序列杂交结合）与构建的预文库杂交，含有HPV基因的序列通过探针上生物素与磁珠结合，可以被特异性杂交；将不含HPV的序列洗涤除去，从而富集HPV基因片段，这样该试剂盒就将HPV序列捕获了出来；通过测序仪Nextseq CN500高通量测序并分析测序结果中的HPV信息，可获得待测样本的HPV基因型别及是否与人基因组发生整合情况。
本发明具有以下有益效果：
（1）本发明设计了可进行快速准确地捕获5种型别HPV基因组全序列的探针组，可以有效的提高检测特异性。以往的荧光定量PCR等方法都是只针对HPV序列的某一段区域，我们的探针检测范围可以覆盖HPV基因组全长。
（2）本发明利用HPV探针组对捕获出目标区域的文库标本利用高通量测序技术测序。由于先使用HPV探针对HPV序列进行了靶向富集捕获，然后再进行高通量测序，使得其检测灵敏度高。
（3）本发明可以一次处理多个样本，通过对多个不同的样本加入不同Barcode进行区分，然后混合上机测序，可以有效的提高检测通量。
（4）本发明可以检测HPV在人基因组中的整合情况，目前市面上针对HPV的试剂盒都是针对HPV分型的，没有可以检测HPV整合的试剂盒。
附图说明：
图1为末端修复、3’端加“A”反应PCR 仪参数；
图2为Pre-PCR 程序参数。
具体实施方式：
以下通过实施例对本发明作进一步阐述。如无特殊说明，本发明所使用的本领域常用试剂均可通过商购途径获得。
实施例1：本发明探针组的设计和制备
根据碱基互补配对原则，针对5种HPV的DNA序列设计与之互补配对的寡核苷酸探针。由于基因组序列结构复杂，存在不规则区域，例如高GC、高AT区域等，同时由于二代测序读长的限制，从而使得探针设计变得尤为关键，需要综合权衡覆盖率、均一性、捕获效率等多种因素。探针设计的具体原则如下：
（1）长度：可选区间80-150bp，一般选100bp。
（2）探针层数：即目标区域上平均被多少条探针所覆盖，一般建议3层，且需叠瓦式设计。
（3）特异性：即探针在基因组范围的唯一性，特异性越高，探针的捕获效率越高。应避免在低复杂度区域设计探针；但在特殊情况下，比如，某些热点恰好位于低复杂度区域，那么需要综合评估该区域以及相邻区域的序列情况，谨慎放置探针。
（4）结合能力：GC含量在50%左右的区域，其探针捕获能力最强。高GC区域探针结合能力强，但DNA片段自身结合能力更强（长度更长），探针竞争阻力较大；高AT区域的DNA片段自身结合能力弱，但同样探针与之结合的能力也弱，因此，在高GC和高AT区域需要适当增加探针数量来弥补竞争阻力和结合能力方面的劣势。
探针制备：先用光化学合成法，在芯片上大量合成设计的探针，再通过PCR或转录反应得到被生物素标记的探针。
实施例2：本发明试剂盒组成、制备及使用
本实施例所述的用于HPV分型及整合检测的试剂盒试剂。
所述试剂盒组成成分包括：本发明所述的用于检测HPV分型及整合检测的捕获探针组、建库缓冲液1、建库缓冲液2、酶1、酶2、接头、PCR扩增引物1、PCR扩增引物2、PCR反应混合液、封闭序列1、封闭序列2、封闭试剂、杂交缓冲液、PCR扩增缓冲液、PCR扩增引物3、PCR扩增反应液、阳性对照、阴性对照、捕获缓冲液、清洗缓冲液1、清洗缓冲液2和捕获磁珠。具体组成成分信息如表2，
所述试剂盒的使用方法为：
1、样本文库制备
（1）片段化：将1000 ng DNA取出解冻后震荡混匀，加水补齐至100μL，利用Bioruptor Pico超声打断仪打断。具体参数设置如下：待冷循环仪温度降至4°C后，设置参数ON 30秒，OFF 30秒为1个循环，每10 个循环为一轮，共进行3 轮；取1 μL样品使用Qsep1进行片段检测，正常打断后样品检测主峰约在150 bp-200 bp。
（2）末端修复加A：将上一步获得的样品进行末端修复、3’端加“A”。具体反应体系如下：35 μL DNA片段，5 μL ERA缓冲液，10 μL末端修复酶。混好后，运行 PCR 程序，设置PCR 仪参数如图1（50 μL体系，热盖85°C）；
（3）加接头：将上一步获得的样本进行接头连接。具体反应体系如下：50 μL上一步反应结束的样品，5 μL接头，15 μL无酶水，20 μL链接缓冲液，10 μL连接酶；混好后，运行PCR仪程序（要求无热盖），设置PCR仪参数：热盖关，20°C 30 分钟，降至4℃结束
（4）磁珠纯化：程序结束后，立即用纯化磁珠纯化上一步反应产物。纯化体系如下：100 μL上一步反应产物，100 μL纯化磁珠。
（5）Pre-PCR扩增反应：以上一步的纯化产物为模板，进行Pre-PCR反应。具体反应体系如下：20 μL上一步的纯化产物，25 μL PCR反应混合液，2.5 μL PCR扩增反应引物1(20 μM)，2.5 μL PCR扩增反应引物2(20 μM)。混好后，将样品置于PCR仪上，启动PCR程序，如图2所示（50 μL体系，热盖温度105°C）；
注：PCR扩增反应引物1中TPE1.0index号为1#~8#（表3）；PCR扩增反应引物2中TPE2.0index号为1#~12#；记录好所使用的index号（表4）。
（6）磁珠纯化：程序结束后，立即用50 μL纯化磁珠纯化上一步反应产物。
2、样本液相杂交
（1）静置解冻封闭序列1和封闭序列2，配制混合液1，具体为：750ng文库，5 μL封闭序列1，2 μL封闭序列2。
（2）取出试剂盒中的封闭试剂和探针，静置解冻后振荡混匀，按照5 μL封闭试剂与2 μL探针配制混合液2（冰上操作）。
（3）取出试剂盒中的杂交缓冲液置于室温化冻，化冻后取出20μL置于65°C恒温混匀仪孵育待用。
（4）将13μL杂交缓冲液添加到混合液2中，吹打混匀后短暂离心收集液体，室温放置待用。
（5）使用可控温真空干燥仪，设置干燥温度45-50℃，打开管盖，直至混合液1浓缩至体积小于10 μL，用无酶水补齐至10μL，轻轻吸打混匀，短暂离心后置于冰上待用。浓缩时间不宜过长，防止蒸干。
（6）将浓缩后的混合液2置于PCR仪中95°C孵育5min，65°C孵育2min（热盖温度为105°C），孵育完成后不需要取出。
（7）将添加了杂交缓冲液的混合液1置于PCR仪中65°C孵育2min（热盖温度为105°C）。
（8）吸取20μL的混合液1添加到混合液2中，充分混匀，65°C杂交16~24 h，保持杂交产物在PCR仪中，进入捕获步骤。
3、捕获
（1）提前取出试剂盒中的磁珠室温平衡30min，振荡混匀。
（2）取出50μL磁珠置于新的反应管中，离心管置于磁力架上静置1min至液体澄清，小心吸弃液体（注意不要吸到磁珠）。
（3）在磁珠中添加200µL捕获缓冲液，吹打混匀后，将离心管置于磁力架上静置1min至液体澄清，小心吸弃液体（注意不要吸到磁珠）。
（4）重复步骤（3）三次。
（5）在磁珠中重新添加200µL捕获缓冲液重悬磁珠，将重悬后的磁珠加入到杂交产物中，并将PCR管置于旋转混旋仪上室温结合30min。
（6）取出试剂盒中的清洗缓冲液1和清洗缓冲液2，清洗缓冲液1室温静置待用，清洗缓冲液2置65℃预热5min以上待用。
（7）将捕获产物置于磁力架上静置1分钟静置1min至液体澄清，小心吸弃液体（注意不要吸到磁珠）。
（8）从磁力架上取下离心管，加入200 μL的清洗缓冲液1，吹打混匀后，旋转混匀仪上15min，放在磁力架上静置1min至液体澄清，小心吸弃液体（注意不要吸到磁珠）。
（9）从磁力架上取下离心管，加入200 μL 65℃预热的清洗缓冲液2，吹打混匀后，置于恒温震荡仪（65℃，800rpm）上10分钟，置于磁力架上静置1min至液体澄清，小心吸弃液体（注意不要吸到磁珠）。
（10）重复步骤（9）三次。
（11）从磁力架上取下离心管，加入200 μL新鲜配制的80%乙醇，置于磁力架上静置30sec至液体澄清，小心吸弃液体（注意不要吸到磁珠）
（12）反应管留于上磁力架室温晾干3~5min使残留乙醇挥发，以磁珠表面无光泽为准（过分晾干将导致DNA得率下降）。
（13）从磁力架上取下离心管，加入30μL无酶水，重悬磁珠待用。
（14）配制混合液3，用0.2mL PCR管，具体反应体系如下：18μLPCR扩增缓冲液，1μLPCR扩增引物，1μLPCR扩增反应液。
（15）将20μL上述反应体系加入到洗涤产物中，充分混匀。
（16）置于PCR仪中按表5反应程序进行PCR扩增。
（17）扩增后纯化，使用商品化的核酸纯化磁珠进行扩增产物纯化，具体操作步骤请按照试剂盒说明书指引进行。吸取全部纯化产物置于冰上，重复2-3步骤，进入测序阶段或于-20±5℃保存。
4、捕获文库质控
（1）使用核酸定量试剂盒QubitdsDNA HS Assay Kit 及配套仪器测定文库的浓度，文库浓度应大于1ng/μL。否则文捕获文库不符合要求，应重新进行杂交捕获。
（2）取样本文库或对照品文库，使用Standard Cartridge Kit(S2)及配套仪器测定文库片段大小，文库片段大小应集中在250bp～500bp之间，无明显小片段和大片段杂峰。否则建库样品不符合要求，应重新进行杂交捕获。
5、文库qPCR定量
使用KAPA Library Quantification Kit试剂盒对待上机富集文库进行定量，以调整至合适上机浓度。
（1）试剂准备：1）准备适量的DNA稀释缓冲液：使用无酶水将1XIDTE缓冲液稀释至0.1X，每个文库约需1.2mL稀释缓冲液。使用前将缓冲液放至室温。2）冰上解冻试剂盒中各组分，使用前充分混匀，短暂离心，置于冰上备用。
（2）样本准备：1）准备适当的文库稀释液（使用无酶水）。取1μL富集文库进行1：100、1：10000、1：20000稀释。
（3）向PCR反应板需要进行反应的孔中各加入混有引物的8μL混合液，混合液具体组成为：5μLMaster Mix，1μL引物，1.8μL水，0.2μLLow Rox。
（4）向每个样本孔中加入对应的2μL已稀释的DNA文库（体积比为1:1000和1:20000）。
（5）向标准品孔中各加入2μL DNA标准品，按照从低浓度到高浓度顺序加入。
（6）向阴性对照孔中加入2μL稀释缓冲液。
（7）密封PCR 反应板，放入微孔板离心机中离心1min。
（8）将反应板装载到qPCR仪上并按照下表6参数运行qPCR仪。
（9）按照KAPA Library Quantification Kit试剂盒说明书进行待检文库的浓度计算。若最终文库总摩尔质量<0.02p 摩尔，需要重新进行文库杂交富集或重新进行建库，若再次不合格，终止检测。
6、上机测序
在基因测序仪Nextseq CN500上选用测序读长为300循环（Paired-End Reads，2×150 cycles）的测序试剂进行测序，建议每个样品文库测序数据量800M。待测序样本文库数量不高于48个，推荐使用NextSeq 500 Mid Output v2 Reagent kit (300 cycles) 试剂检测；待测序样本文库数量高于48个且不高于96个，推荐使NextSeq 500 High Output v2Reagent kit (300 cycles) 试剂检测。
（1）提前取出Reagent Cartridge、HT1和Flow Cell，Flow Cell置于室温平衡30min，Reagent Cartridge置于室温水浴1h，HT1室温静置解冻后振荡混匀。
（2）将所有待测序样本文库按测序数据量需求进行混合，使用核酸定量试剂盒QubitdsDNA HS Assay Kit及配套仪器稀释各文库浓度至4 nM，取一个新的离心管等体积取各文库混合，混合后的总体积不低于5μL。
（3）配制新鲜的0.2N NaOH，由1μL 1N NaOH加入到4μL纯化水中混匀得到。
（4）取5μL 4 nM混合文库至新的1.5 mL离心管中，加入5μL新鲜配制的0.2N NaOH，吹打混匀室温静置5min进行4 nM混合文库变性，变性后加入混匀后的990μLHT1将混合文库稀释至20 pM。
（5）取一个新的1.5 mL离心管加入混匀后的1365μL HT1。
（6）取135μL稀释至20 pM的混合文库加入至1365μL HT1中，使最终上机混合文库总体积为1500μL。
（7）取出室温水浴解冻的Reagent Cartridge，用无尘纸檫干，翻转5次混匀其中试剂。
（8）使用干净的1 mL移液器吸头戳破Reagent Cartridge上带有Load Samples标签的10号孔封箔口，将1300μL混合有PhiX对照品的最终上机混合文库注入10号孔。
（9）根据测序仪运行设置提示进行试剂加载操作，测序仪自检完成后，点击“开始”按钮进行测序，测序时长26-29h。
7、HPV核酸分型与整合生物信息分析流程
（1）测序完成后，使用illumina公司的bcl2fastq v2.20.0.422软件将测序生成的BCL文件转化成样本对应的fastq文件。
（2）使用illuminate v0.6软件读取测序文件中InterOp目录的记录信息，对本次测序进行质量分析与评估。Fastp v0.20.0进行质控，要求Q30质量值大于80%。
（3）质量评估完成后，预处理数据，根据fastq文件碱基及质量，对原始数据进行质量控制（基于fastp v0.20.0软件）。
（4）序列比对：将fastq文件中的碱基序列比对至人类参考基因组hg38（GRCh38）和HPV基因组上生成bam文件（基于BWA v0.7.17、samtoolsv1.9和picard v2.20.6软件：比对）。
（5）HPV型別分析：使用分型检测模块（基于hpvHPVkitTyper v1.0软件），通过序列比对文件分析样本中各种HPV的reads数和比例。
（6）HPV整合分析：使用整合检测模块（基于hpvkitHPVFusionCallerv1软件），通过序列比对文件中比对上HPV和人基因组的序列进行位点比较，分析样本中HPV整合位点，并结合数据库进行注释。
（7）结果判定：分型分析，单个HPV型別Mean depth≥10则判定为该型别分型阳性；整合分析，单位数据量中HPV整合read数≥3则判定为该型别整合阳性。
实施例3：本发明试剂盒的使用效果验证
下面通过具体实施方式对本发明做进一步的详细说明。
1、20例参考品样本和16例临床样本HPV分型整合的高通量测序检测
样本编号为S1~S36,其中S1~S18为10^4拷贝 /mL的分型质粒参考品，分别为HPV16、18、26、31、33、35、39、45、51、52、53、56、58、59、66、68、73、82，S19和S20分别是浓度为0.25ng/μL的SiHa细胞（已知的HPV16整合样本）和HeLa细胞DNA（已知的HPV18整合样本），S21~S36为临床样本。检测结果：我们利用本发明的方法及试剂盒检测到了参考品样本对应的HPV型别及整合状态，说明本发明可对实际样本进行准确快速的检测，有实际可使用价值。本发明提供的用于HPV分型及整合检测的探针组及试剂盒具有操作简便、高通量、特异性好、灵敏度高等特点。结果如下：表7为36例样本高通量测序质控结果，表8为36例样本高通量测序分型结果，表9为18例样本高通量测序整合结果。
/>
/>
/>
SEQUENCE LISTING
<110> 武汉凯德维斯生物技术有限公司
<120> 一种用于检测5种人***瘤病毒HPV分型和整合的探针组及其试剂盒
<130> 0
<160> 18
<170> PatentIn version 3.5
<210> 1
<211> 25221
<212> DNA
<213> Artificial Sequence
<220>
<223> 探针型别HPV16
<400> 1
ctacaataat tcatgtataa aactaagggc gtaaccgaaa tcggttgaac cgaaaccggt 60
tagtataaaa gcagacattt tcgtaaccaa aagagaactg aaaccggtta gtataaaagc 120
agacattttc gtaaccaaaa gagaactgca atgtttcagg acccacagga gcgacccaga 180
aagttaccac agttcgtaac gcaccaaaag agaactgcaa tgtttcagga cccacaggag 240
cgacccagaa agttaccaca gttcgtaaca gagctgcaaa caactataca tgatataata 300
aggagcgacc cagaaagtta ccacagttcg taacagagct gcaaacaact atacatgata 360
taatattaga atgtgtgtac tgcaagcaac agttactgcg cagagctgca aacaactata 420
catgatataa tattagaatg tgtgtactgc aagcaacagt tactgcgacg tgaggtatat 480
gactttgctt ttcgggattt tagaatgtgt gtactgcaag caacagttac tgcgacgtga 540
ggtatatgac tttgcttttc gggatttcgt aatagtatat agagatggga atccatcgta 600
gcgacgtgag gtatatgact ttgcttttcg ggatttcgta atagtatata gagatgggaa 660
tccatcgtat gtatgtgata aatgtttaaa gttttattct gtgaggtata tgactttgct 720
tttcgggatt tcgtaatagt atatagagat gggaatccat cgtatgtatg tgataaatgt 780
ttaaagtttt attccgtaat agtatataga gatgggaatc catcgtatgt atgtgataaa 840
tgtttaaagt tttattctaa aattagtgag tatagacatt attgttatag tttggctgta 900
tgtgataaat gtttaaagtt ttattctaaa attagtgagt atagacatta ttgttatagt 960
ttgtatggaa caacattaga acagcaatac aacatctaaa attagtgagt atagacatta 1020
ttgttatagt ttgtatggaa caacattaga acagcaatac aacaaaccgt tgtgtgattt 1080
gttaattagg tgtaaattag tgagtataga cattattgtt atagtttgta tggaacaaca 1140
ttagaacagc aatacaacaa accgttgtgt gatttgttaa ttaggtgtat taactgtatg 1200
gaacaacatt agaacagcaa tacaacaaac cgttgtgtga tttgttaatt aggtgtatta 1260
actgtcaaaa gccactgtgt cctgaagaaa agcaaaccgt tgtgtgattt gttaattagg 1320
tgtattaact gtcaaaagcc actgtgtcct gaagaaaagc aaagacatct ggacaaaaag 1380
caaagattcc ataaactgtc aaaagccact gtgtcctgaa gaaaagcaaa gacatctgga 1440
caaaaagcaa agattccata atataagggg tcggtggacc ggtcgatgta tgtcagacat 1500
ctggacaaaa agcaaagatt ccataatata aggggtcggt ggaccggtcg atgtatgtct 1560
tgttgcagat catcaagaac acgtagagaa acccatataa ggggtcggtg gaccggtcga 1620
tgtatgtctt gttgcagatc atcaagaaca cgtagagaaa cccagctgta atccgtaatg 1680
gagatacacc tacacttgtt gcagatcatc aagaacacgt agagaaaccc agctgtaatc 1740
cgtaatggag atacacctac attgcatgaa tatatgttag atttgcaacc agagcccagc 1800
tgtaatccgt aatggagata cacctacatt gcatgaatat atgttagatt tgcaaccaga 1860
gacaactgat ctctactgtt atgagcaatt aaattgcatg aatatatgtt agatttgcaa 1920
ccagagacaa ctgatctcta ctgttatgag caattaaatg acagctcaga ggaggaggat 1980
gaaatagatg gtccgacaac tgatctctac tgttatgagc aattaaatga cagctcagag 2040
gaggaggatg aaatagatgg tccagctgga caagcagaac cggacagagc ccatactgtt 2100
atgagcaatt aaatgacagc tcagaggagg aggatgaaat agatggtcca gctggacaag 2160
cagaaccgga cagagcccat tacaatgaca gctcagagga ggaggatgaa atagatggtc 2220
cagctggaca agcagaaccg gacagagccc attacaatat tgtaaccttt tgttgcaagt 2280
gtgacccagc tggacaagca gaaccggaca gagcccatta caatattgta accttttgtt 2340
gcaagtgtga ctctacgctt cggttgtgcg tacaaagcac acacgcaata ttgtaacctt 2400
ttgttgcaag tgtgactcta cgcttcggtt gtgcgtacaa agcacacacg tagacattcg 2460
tactttggaa gacctgttaa tgggccgtag acattcgtac tttggaagac ctgttaatgg 2520
gcacactagg aattgtgtgc cccatctgtt ctcagaaacc ataatctacc atggctgatc 2580
ctgcagggca cactaggaat tgtgtgcccc atctgttctc agaaaccata atctaccatg 2640
gctgatcctg caggtaccaa tggggaagag ggtacgggat gtaatctcag aaaccataat 2700
ctaccatggc tgatcctgca ggtaccaatg gggaagaggg tacgggatgt aatggatggt 2760
tttatgtaga ggctgtagtg gaaaaggtac caatggggaa gagggtacgg gatgtaatgg 2820
atggttttat gtagaggctg tagtggaaaa aaaaacaggg gcgtatatat cagatgacga 2880
gaacgggatg gttttatgta gaggctgtag tggaaaaaaa aacaggggcg tatatatcag 2940
atgacgagaa cgaaaatgac agtgatacag gtgaagattt ggtagaaaac aggggcgtat 3000
atatcagatg acgagaacga aaatgacagt gatacaggtg aagatttggt agattttata 3060
gtaaatgata atgattattt aacaccgaaa atgacagtga tacaggtgaa gatttggtag 3120
attttatagt aaatgataat gattatttaa cacaggcaga aacagagaca gcaccgtagt 3180
tgtttgattt tatagtaaat gataatgatt atttaacaca ggcagaaaca gagacagcac 3240
cgtagttgtt tactgcacag gaagcaaaac aacatagagc gtaagcacag gcagaaacag 3300
agacagcacc gtagttgttt actgcacagg aagcaaaaca acatagagcg taagtacagg 3360
ttctaaaacg aaagtatttg ggtagtactg cacaggaagc aaaacaacat agagcgtaag 3420
tacaggttct aaaacgaaag tatttgggta gtccacttag tgatattagt ggatgtgtag 3480
acaatgcagt acaggttcta aaacgaaagt atttgggtag tccacttagt gatattagtg 3540
gatgtgtaga caataatatt agtcctagat taaaagctat atgtagtcca cttagtgata 3600
ttagtggatg tgtagacaat aatattagtc ctagattaaa agctatatgt atagaaaaac 3660
aaagtagagc tgcaaaaagg agattaatat tagtcctaga ttaaaagcta tatgtataga 3720
aaaacaaagt agagctgcaa aaaggagatt atttgaaagc gaagacagcg ggtatggcaa 3780
tactgttaaa agctatatgt atagaaaaac aaagtagagc tgcaaaaagg agattatttg 3840
aaagcgaaga cagcgggtat ggcaatactg aagtggagaa aaacaaagta gagctgcaaa 3900
aaggagatta tttgaaagcg aagacagcgg gtatggcaat actgaagtgg aaactcagca 3960
gatgttacag gtagaagatt tgaaagcgaa gacagcgggt atggcaatac tgaagtggaa 4020
actcagcaga tgttacaggt agaagggcgc catgagactg aaacaccatg tagtcagtga 4080
agtggaaact cagcagatgt tacaggtaga agggcgccat gagactgaaa caccatgtag 4140
tcagtatagt ggtggaagtg ggggtggttg cagtcagagg gcgccatgag actgaaacac 4200
catgtagtca gtatagtggt ggaagtgggg gtggttgcag tcagtacagt agtggaagtg 4260
ggggagaggg tgttagtgta tagtggtgga agtgggggtg gttgcagtca gtacagtagt 4320
ggaagtgggg gagagggtgt tagtgaaaga cacactatcg tacaaacacc acttacagta 4380
cagtagtgga agtgggggag agggtgttag tgaaagacac actatcgtac aaacaccact 4440
tacaaatatt ttaaatgtac taaaaactag tacgtaagtg aaagacacac tatcgtacaa 4500
acaccactta caaatatttt aaatgtacta aaaactagta cgtaaaaggc agcaatgtta 4560
gcaaaattta aagagttcaa atattttaaa tgtactaaaa actagtacgt aaaaggcagc 4620
aatgttagca aaatttaaag agttatacgg ggtgagtttt tcagaattag taagaccaaa 4680
ggcagcaatg ttagcaaaat ttaaagagtt atacggggtg agtttttcag aattagtaag 4740
accatttaaa agtaataaat caacgtgttg cgattggacg gggtgagttt ttcagaatta 4800
gtaagaccat ttaaaagtaa taaatcaacg tgttgcgatt ggtgtattgc tgcatttgga 4860
cttacaccca gtatagcggt gtattgctgc atttggactt acacccagta tagctgacag 4920
tataaaaaca ctattacaac aatattgttt atatttacac attcaaagtt tagcatgagc 4980
tgacagtata aaaacactat tacaacaata ttgtttatat ttacacattc aaagtttagc 5040
atgttcatgg ggaatggttg tgttactatt agtaagattg tttatattta cacattcaaa 5100
gtttagcatg ttcatgggga atggttgtgt tactattagt aagatataaa tgtggaaaaa 5160
atagagaaac aattgaagtt catggggaat ggttgtgtta ctattagtaa gatataaatg 5220
tggaaaaaat agagaaacaa ttgaaaaatt gctgtctaaa ctattatgtg tgtctccgat 5280
ataaatgtgg aaaaaataga gaaacaattg aaaaattgct gtctaaacta ttatgtgtgt 5340
ctccaatgtg tatgatgata gagcctccaa aattgcgaat tgctgtctaa actattatgt 5400
gtgtctccaa tgtgtatgat gatagagcct ccaaaattgc gtagtacagc agcagcatta 5460
tattggtata aaacaggaat gtgtatgatg atagagcctc caaaattgcg tagtacagca 5520
gcagcattat attggtataa aacaggtata tcaaatatta gtgaagtgta tggagaccca 5580
aaattgcgta gtacagcagc agcattatat tggtataaaa caggtatatc aaatattagt 5640
gaagtgtatg gagacacgcc agaatggata caaagacgca ttatattggt ataaaacagg 5700
tatatcaaat attagtgaag tgtatggaga cacgccagaa tggatacaaa gacaaacagt 5760
attacaacat agttatattg gtataaaaca ggtatatcaa atattagtga agtgtatgga 5820
gacacgccag aatggataca aagacaaaca gtattacaac atagcaggta tatcaaatat 5880
tagtgaagtg tatggagaca cgccagaatg gatacaaaga caaacagtat tacaacatag 5940
ttttaatgat tgtacatttg aattcacgcc agaatggata caaagacaaa cagtattaca 6000
acatagtttt aatgattgta catttgaatt atcacagatg gtacaatggg cctacgataa 6060
tgaccaacat agttttaatg attgtacatt tgaattatca cagatggtac aatgggccta 6120
cgataatgac atagtagacg atagtgaaat tgcatataaa tatgcacaga tggtacaatg 6180
ggcctacgat aatgacatag tagacgatag tgaaattgca tataaatcgt aacaattggc 6240
agacactaat agtacgtaaa gtgcggtaca atgggcctac gataatgaca tagtagacga 6300
tagtgaaatt gcatataaat cgtaacaatt ggcagacact aatagtacgt aaagtgccat 6360
agtagacgat agtgaaattg catataaatc gtaacaattg gcagacacta atagtacgta 6420
aagtgccttt ctaaaaagta attcacaggc aaaaattgat agtgaaattg catataaatc 6480
gtaacaattg gcagacacta atagtacgta aagtgccttt ctaaaaagta attcacaggc 6540
aaaaattgta aaggattgca caattggcag acactaatag tacgtaaagt gcctttctaa 6600
aaagtaattc acaggcaaaa attgtaaagg attgtgcaac aatgtgtaga cattatggca 6660
gacactaata gtacgtaaag tgcctttcta aaaagtaatt cacaggcaaa aattgtaaag 6720
gattgtgcaa caatgtgtag acattataaa cgagcgcctt tctaaaaagt aattcacagg 6780
caaaaattgt aaaggattgt gcaacaatgt gtagacatta taaacgagca gaaaaaaaac 6840
aaatgagtat gagtcgtaaa ggattgtgca acaatgtgta gacattataa acgagcagaa 6900
aaaaaacaaa tgagtatgag tcaatggata aaatatagat gtgatagggt agatgcaaca 6960
atgtgtagac attataaacg agcagaaaaa aaacaaatga gtatgagtca atggataaaa 7020
tatagatgtg atagggtaga tgatggtaaa cgagcagaaa aaaaacaaat gagtatgagt 7080
caatggataa aatatagatg tgatagggta gatgatggag gtgattggaa gcaaattgtt 7140
atgtttgtca atggataaaa tatagatgtg atagggtaga tgatggaggt gattggaagc 7200
aaattgttat gtttttaagg tatcaaggtg tagagtttat gtcattgatg gaggtgattg 7260
gaagcaaatt gttatgtttt taaggtatca aggtgtagag tttatgtcat ttttaactgc 7320
attaaaaaga tttttgcaag gcatactaag gtatcaaggt gtagagttta tgtcattttt 7380
aactgcatta aaaagatttt tgcaaggcat acctaaaaaa aattgcatat tactatatgg 7440
tgcagcttta actgcattaa aaagattttt gcaaggcata cctaaaaaaa attgcatatt 7500
actatatggt gcagctaaca caggtaaatc attatttggt atgagtacct aaaaaaaatt 7560
gcatattact atatggtgca gctaacacag gtaaatcatt atttggtatg agtttaatga 7620
aatttctgca agggtctgta atatgtgcag ctaacacagg taaatcatta tttggtatga 7680
gtttaatgaa atttctgcaa gggtctgtaa tatgttttgt aaattctaaa agccattttt 7740
ggttacgttt aatgaaattt ctgcaagggt ctgtaatatg ttttgtaaat tctaaaagcc 7800
atttttggtt acaaccatta gcagcgtaca aaataggtat gttagagttt tgtaaattct 7860
aaaagccatt tttggttaca accattagca gcgtacaaaa taggtatgtt agatgcgtat 7920
acagtgccct gttggaacta catagaccat tagcagcgta caaaataggt atgttagatg 7980
cgtatacagt gccctgttgg aactacatag atgacaattt aagaacgtaa ttggatggaa 8040
atttaggcgt atacagtgcc ctgttggaac tacatagatg acaatttaag aacgtaattg 8100
gatggaaatt tagtttctat ggatgtaaag catagaccat tggtactgac aatttaagaa 8160
cgtaattgga tggaaattta gtttctatgg atgtaaagca tagaccattg gtacaactaa 8220
acgtacctcc attattaatt acatctagtt tctatggatg taaagcatag accattggta 8280
caactaaacg tacctccatt attaattaca tctaacatta cgtatggtac agattctagg 8340
tggcctcaac taaacgtacc tccattatta attacatcta acattacgta tggtacagat 8400
tctaggtggc cttatttaca taatagattg gtggtgttta catttcacat tacgtatggt 8460
acagattcta ggtggcctta tttacataat agattggtgg tgtttacatt tcctaatgag 8520
tttccatttg acgaaaacgg aaatcctcct aatgagtttc catttgacga aaacggaaat 8580
ccagtgtatg agcttaatga taagaactgg aaatcctttt tctcaaggac gtggtccaga 8640
ttaagttttc catttgacga aaacggaaat ccagtgtatg agcttaatga taagaactgg 8700
aaatcctttt tctcaaggac gtggtccaga ttaagtttgc acgaggccag tgtatgagct 8760
taatgataag aactggaaat cctttttctc aaggacgtgg tccagattaa gtttgcacga 8820
ggacgaggac aaggaaaacg atggagtcct ttttctcaag gacgtggtcc agattaagtt 8880
tgcacgagga cgaggacaag gaaaacgatg gagactcttt gccaacgttt aaatgtgtgt 8940
caggactcct ttttctcaag gacgtggtcc agattaagtt tgcacgagga cgaggacaag 9000
gaaaacgatg gagactcttt gccaacgttt aaatgtgtgt caggacctca aggacgtggt 9060
ccagattaag tttgcacgag gacgaggaca aggaaaacga tggagactct ttgccaacgt 9120
ttaaatgtgt gtcaggacaa aatactagga cgtggtccag attaagtttg cacgaggacg 9180
aggacaagga aaacgatgga gactctttgc caacgtttaa atgtgtgtca ggacaaaata 9240
ctatttgcac gaggacgagg acaaggaaaa cgatggagac tctttgccaa cgtttaaatg 9300
tgtgtcagga caaaatacta acacattatg aaaatgatag tacgagactc tttgccaacg 9360
tttaaatgtg tgtcaggaca aaatactaac acattatgaa aatgatagta cagacctacg 9420
tgaccatata gactattgga aacgactctt tgccaacgtt taaatgtgtg tcaggacaaa 9480
atactaacac attatgaaaa tgatagtaca gacctacgtg accatataga ctattggaaa 9540
caccaaaata ctaacacatt atgaaaatga tagtacagac ctacgtgacc atatagacta 9600
ttggaaacac cgtagcctag aatgtgctat ttattacaag gcccagacct acgtgaccat 9660
atagactatt ggaaacaccg tagcctagaa tgtgctattt attacaaggc cagagaaatg 9720
ggatttaaac atattaacca ccacaccgta gcctagaatg tgctatttat tacaaggcca 9780
gagaaatggg atttaaacat attaaccacc aggtggtgcc aacactggct gtatcaaaga 9840
ataggccaga gaaatgggat ttaaacatat taaccaccag gtggtgccaa cactggctgt 9900
atcaaagaat aaagcattac aagcaattga actgcaacta acgcaggtgg tgccaacact 9960
ggctgtatca aagaataaag cattacaagc aattgaactg caactaacgt tagaaacaat 10020
atataactca caatatagta atgagcatta caagcaattg aactgcaact aacgttagaa 10080
acaatatata actcacaata tagtaatgaa aagtggacat tacaagacgt tagccttgaa 10140
gtgtagaaac aatatataac tcacaatata gtaatgaaaa gtggacatta caagacgtta 10200
gccttgaagt gtatttaact gcaccaacag gatgtataaa aaaaaaagtg gacattacaa 10260
gacgttagcc ttgaagtgta tttaactgca ccaacaggat gtataaaaaa acatggatat 10320
acagtggaag tgcagtttga tgggtattta actgcaccaa caggatgtat aaaaaaacat 10380
ggatatacag tggaagtgca gtttgatgga gacatcgtaa atacacgtaa ttatacaaac 10440
tggaacatgg atatacagtg gaagtgcagt ttgatggaga catcgtaaat acacgtaatt 10500
atacaaactg gacacatata tatatttgtg aagaagcatc agtgagacat cgtaaataca 10560
cgtaattata caaactggac acatatatat atttgtgaag aagcatcagt aactgtggta 10620
gagggtcaag ttgactatta tggggacaca tatatatatt tgtgaagaag catcagtaac 10680
tgtggtagag ggtcaagttg actattatgg tttatattat gttcatgaag gaatacgaac 10740
atagtaactg tggtagaggg tcaagttgac tattatggtt tatattatgt tcatgaagga 10800
atacgaacat attttgtgca gtttaaagat gcgtaagaaa aatgtttata ttatgttcat 10860
gaaggaatac gaacatattt tgtgcagttt aaagatgcgt aagaaaaata tagtaaaaat 10920
aaagtatggg aagttccgta gggattttgt gcagtttaaa gatgcgtaag aaaaatatag 10980
taaaaataaa gtatgggaag ttccgtaggg tggtcaggta atattatgtc ctacatctgt 11040
gttatagtaa aaataaagta tgggaagttc cgtagggtgg tcaggtaata ttatgtccta 11100
catctgtgtt tagcagcaac gaagtatcct ctcctgaaat tatgtggtca ggtaatatta 11160
tgtcctacat ctgtgtttag cagcaacgaa gtatcctctc ctgaaattat taggcagcac 11220
ttggccaacc accccgccgc gacttagcag caacgaagta tcctctcctg aaattattag 11280
gcagcacttg gccaaccacc ccgccgcgac ccataccaaa gccgtcgcct tgggcaccga 11340
agattaggca gcacttggcc aaccaccccg ccgcgaccca taccaaagcc gtcgccttgg 11400
gcaccgaaga aacacagacg actatccagc gaccaagatc agacccatac caaagccgtc 11460
gccttgggca ccgaagaaac acagacgact atccagcgac caagatcaga gccagacacc 11520
ggaaacccct gccacaccac taaaaacaca gacgactatc cagcgaccaa gatcagagcc 11580
agacaccgga aacccctgcc acaccactaa gttgttgcac agagactcag tggacagtgc 11640
tccagccaga caccggaaac ccctgccaca ccactaagtt gttgcacaga gactcagtgg 11700
acagtgctcc aatcctcact gcatttaaca gctcacacaa aggagttgtt gcacagagac 11760
tcagtggaca gtgctccaat cctcactgca tttaacagct cacacaaagg acggattaac 11820
tgtaatagta acactacacc catccaatcc tcactgcatt taacagctca cacaaaggac 11880
ggattaactg taatagtaac actacaccca tagtacattt aaaaggtgcg tataatactt 11940
taagacggat taactgtaat agtaacacta cacccatagt acatttaaaa ggtgcgtata 12000
atactttaaa atgtttaaga tatagattta aaaagcattg tacagtacat ttaaaaggtg 12060
cgtataatac tttaaaatgt ttaagatata gatttaaaaa gcattgtaca ttgtatactg 12120
cagtgtcgtc tacatggcat tggaaggtgc gtataatact ttaaaatgtt taagatatag 12180
atttaaaaag cattgtacat tgtatactgc agtgtcgtct acatggcatt ggacaaatgt 12240
ttaagatata gatttaaaaa gcattgtaca ttgtatactg cagtgtcgtc tacatggcat 12300
tggacaggac ataatgtaaa acataaaagt gcaatcattg tatactgcag tgtcgtctac 12360
atggcattgg acaggacata atgtaaaaca taaaagtgca attgttacac ttacatatga 12420
tagtgaatgg caacgggaca ggacataatg taaaacataa aagtgcaatt gttacactta 12480
catatgatag tgaatggcaa cgtgaccaat ttttgtctca agttaaaata ccaaattgtt 12540
acacttacat atgatagtga atggcaacgt gaccaatttt tgtctcaagt taaaatacca 12600
aaaactatta cagtgtctac tggatttatg tctatcgtga ccaatttttg tctcaagtta 12660
aaataccaaa aactattaca gtgtctactg gatttatgtc tatatgacaa atcttgatac 12720
tgcatccaca acattaaact attacagtgt ctactggatt tatgtctata tgacaaatct 12780
tgatactgca tccacaacat tactggcgtg ctttttgctt tgcttttgtg tgctttatga 12840
caaatcttga tactgcatcc acaacattac tggcgtgctt tttgctttgc ttttgtgtgc 12900
ttttgtgtgt ctgcctatta atacgtccgc tgcttactgg cgtgcttttt gctttgcttt 12960
tgtgtgcttt tgtgtgtctg cctattaata cgtccgctgc ttttgtctgt gtctacatac 13020
acatcattaa taatagtgtg tctgcctatt aatacgtccg ctgcttttgt ctgtgtctac 13080
atacacatca ttaataatat tggtattact attgtggata acagcagcct ctgcgttgtc 13140
tgtgtctaca tacacatcat taataatatt ggtattacta ttgtggataa cagcagcctc 13200
tgcgtttagg tgttttattg tatatattat atttgtggta ttactattgt ggataacagc 13260
agcctctgcg tttaggtgtt ttattgtata tattatattt gtttatatac cattattttt 13320
aatacataca ccgtatgttt atataccatt atttttaata catacaccgt aacgcttttt 13380
aattacataa tgtatatgta cataatgtaa ttgttacata taattgttgt atacctgcac 13440
gctttttaat tacataatgt atatgtacat aatgtaattg ttacatataa ttgttgtata 13500
ccataactta ctattttttc ttttttattt tcataatttt ttctttttta ttttcatata 13560
taattttttt ttttgtttgt ttgtttgttt tttaataaac tgttattact taacacgtag 13620
acacaaacgt tctgctaata aactgttatt acttaacacg tagacacaaa cgttctgcaa 13680
aacgcacaaa acgtgcatcg gctacccaac tttataaaac cgtaaaacag gcaggcaaac 13740
gttctgcaaa acgcacaaaa cgtgcatcgg ctacccaact ttataaaacc gtaaaacagg 13800
caggtacatg tccacctgac attataccta aggttcggct acccaacttt ataaaaccgt 13860
aaaacaggca ggtacatgtc cacctgacat tatacctaag gttgaaggca aaactattgc 13920
tgatcaaata ttacaggcag gtacatgtcc acctgacatt atacctaagg ttgaaggcaa 13980
aactattgct gatcaaatat tacaatatgg aagtatgggt gtattttttg gtgggtgaag 14040
gcaaaactat tgctgatcaa atattacaat atggaagtat gggtgtattt tttggtgggt 14100
taggaattgg aacagggtcg ggtacaggcg gacgcatatg gaagtatggg tgtatttttt 14160
ggtgggttag gaattggaac agggtcgggt acaggcggac gcactgggta tattccattg 14220
ggaacaaggc ctcccttagg aattggaaca gggtcgggta caggcggacg cactgggtat 14280
attccattgg gaacaaggcc tcccacagct acagatacac ttgctcctgt aagaccactg 14340
ggtatattcc attgggaaca aggcctccca cagctacaga tacacttgct cctgtaagac 14400
cccctttaac agtagatcct gtgggccctt ctgatccaca gctacagata cacttgctcc 14460
tgtaagaccc cctttaacag tagatcctgt gggcccttct gatccttcta tagtttcttt 14520
agtggaagaa actagcccct ttaacagtag atcctgtggg cccttctgat ccttctatag 14580
tttctttagt ggaagaaact agttttattg cgtatggtgc accaacatct gtacctcctt 14640
ctatagtttc tttagtggaa gaaactagtt ttattgcgta tggtgcacca acatctgtac 14700
cttccattcc cccagatgta tcaggattta gtattgtttt attgcgtatg gtgcaccaac 14760
atctgtacct tccattcccc cagatgtatc aggatttagt attactactt caactgatac 14820
cacacctgct atatttccat tcccccagat gtatcaggat ttagtattac tacttcaact 14880
gataccacac ctgctatatt agatattaat aatactgtta ctactgttac tacactacta 14940
cttcaactga taccacacct gctatattag atattaataa tactgttact actgttacta 15000
cacataataa tcccactttc actgacccat ctgtagatat taataatact gttactactg 15060
ttactacaca taataatccc actttcactg acccatctgt attgcagcct ccaacacctg 15120
cagaaactgg agggccacat aataatccca ctttcactga cccatctgta ttgcagcctc 15180
caacacctgc agaaactgga gggcatttta cactttcatc atccactatt agtacattgc 15240
agcctccaac acctgcagaa actggagggc attttacact ttcatcatcc actattagta 15300
cacataatta tgaagaaatt cctatggata catttggcat tttacacttt catcatccac 15360
tattagtaca cataattatg aagaaattcc tatggataca tttattgtta gcacaaaccc 15420
taacacagta actagcacat aattatgaag aaattcctat ggatacattt attgttagca 15480
caaaccctaa cacagtaact agtagcacac ccataccagg gtctcgccca gtggcattgt 15540
tagcacaaac cctaacacag taactagtag cacacccata ccagggtctc gcccagtggc 15600
acgcctagga ttatatagtc gcacaacaca acaggagcac acccatacca gggtctcgcc 15660
cagtggcacg cctaggatta tatagtcgca caacacaaca ggttaaagtt gtagaccctg 15720
cttttgtaac cactcgtggc acgcctagga ttatatagtc gcacaacaca acaggttaaa 15780
gttgtagacc ctgcttttgt aaccactccc actaaactta ttacatatga taatccacgc 15840
ctaggattat atagtcgcac aacacaacag gttaaagttg tagaccctgc ttttgtaacc 15900
actcccacta aacttattac atatgataat cctgccacgc ctaggattat atagtcgcac 15960
aacacaacag gttaaagttg tagaccctgc ttttgtaacc actcccacta aacttattac 16020
atatgataat cctgcggtta aagttgtaga ccctgctttt gtaaccactc ccactaaact 16080
tattacatat gataatcctg catatgaagg tatagatgtg gataatacat tatatctccc 16140
actaaactta ttacatatga taatcctgca tatgaaggta tagatgtgga taatacatta 16200
tatttttcta gtaatgataa tagtattaat atagcgcata tgaaggtata gatgtggata 16260
atacattata tttttctagt aatgataata gtattaatat agctccagat cctgactttt 16320
tggatatagt tgcttttttt ctagtaatga taatagtatt aatatagctc cagatcctga 16380
ctttttggat atagttgctt tacataggcc agcattaacc tctaggcgta ctggcccaga 16440
tcctgacttt ttggatatag ttgctttaca taggccagca ttaacctcta ggcgtactgg 16500
cattaggtac agtagaattg gtaataaaca aacacacata ggccagcatt aacctctagg 16560
cgtactggca ttaggtacag tagaattggt aataaacaaa cactacgtac tcgtagtgga 16620
aaatctatag gtgctggcat taggtacagt agaattggta ataaacaaac actacgtact 16680
cgtagtggaa aatctatagg tgctaaggta cattattatt atgatttaag tactacacta 16740
cgtactcgta gtggaaaatc tataggtgct aaggtacatt attattatga tttaagtact 16800
attgatcctg cagaagaaat agaattacaa actatggtac attattatta tgatttaagt 16860
actattgatc ctgcagaaga aatagaatta caaactataa caccttctac atatactacc 16920
acttcaccgt aagccgatcc tgcagaagaa atagaattac aaactataac accttctaca 16980
tatactacca cttcaccgta agcctcacct acttctatta ataatggatt atatgaacac 17040
cttctacata tactaccact tcaccgtaag cctcacctac ttctattaat aatggattat 17100
atgatatttc gtaagatgac tttattacag atactcagcc tcacctactt ctattaataa 17160
tggattatat gatatttcgt aagatgactt tattacagat acttctacaa ccccggtacc 17220
atctgtaccc tctactgata tttcgtaaga tgactttatt acagatactt ctacaacccc 17280
ggtaccatct gtaccctcta catctttatc aggttatatt cctgcaaata caacatctac 17340
aaccccggta ccatctgtac cctctacatc tttatcaggt tatattcctg caaatacaac 17400
aattcctttt ggtggtgcat acaatattcc tttagcatct ttatcaggtt atattcctgc 17460
aaatacaaca attccttttg gtggtgcata caatattcct ttagtatcag gtcctgatat 17520
acccattaat ataactcctt ttggtggtgc atacaatatt cctttagtat caggtcctga 17580
tatacccatt aatataactg accaagctcc ttcattaatt cctatagttc caggggtatc 17640
aggtcctgat atacccatta atataactga ccaagctcct tcattaattc ctatagttcc 17700
agggtctcca caatatacaa ttattgctgc gtaagtgacc aagctccttc attaattcct 17760
atagttccag ggtctccaca atatacaatt attgctgcgt aaggtgactt ttatttacat 17820
cctagttatt acatgccagg gtctccacaa tatacaatta ttgctgcgta aggtgacttt 17880
tatttacatc ctagttatta catgttacga aaacgacgta aacgtttacc atattggtga 17940
cttttattta catcctagtt attacatgtt acgaaaacga cgtaaacgtt taccatattt 18000
tttttcagat gtctctttgg ctgcctagtg aggccgttac gaaaacgacg taaacgttta 18060
ccatattttt tttcagatgt ctctttggct gcctagtgag gccactgtct acttgcctcc 18120
tgtcccagta tctaaaccat attttttttc agatgtctct ttggctgcct agtgaggcca 18180
ctgtctactt gcctcctgtc ccagtatcta aggttgtaag cacggatgaa tatgtttttt 18240
cagatgtctc tttggctgcc tagtgaggcc actgtctact tgcctcctgt cccagtatct 18300
aaggttgtaa gcacggatga atatgttgca cgcacctagt gaggccactg tctacttgcc 18360
tcctgtccca gtatctaagg ttgtaagcac ggatgaatat gttgcacgca caaacatata 18420
ttatccgtaa gggccactgt ctacttgcct cctgtcccag tatctaaggt tgtaagcacg 18480
gatgaatatg ttgcacgcac aaacatatat tatccgtaag gaacatccag acaggttgta 18540
agcacggatg aatatgttgc acgcacaaac atatattatc cgtaaggaac atccagacta 18600
cttgcagttg gacatcccta ttttcctatt aacaaacata tattatccgt aaggaacatc 18660
cagactactt gcagttggac atccctattt tcctattaaa aaacctaaca ataacaaaat 18720
attagttcct aactacttgc agttggacat ccctattttc ctattaaaaa acctaacaat 18780
aacaaaatat tagttcctaa agtatcagga ttacaataca gggtatttag aaaaaaacct 18840
aacaataaca aaatattagt tcctaaagta tcaggattac aatacagggt atttagaata 18900
catttacctg accccaataa gtttggtttt ccgtatcagg attacaatac agggtattta 18960
gaatacattt acctgacccc aataagtttg gttttcctga cacctcattt tataatccag 19020
atacacagcg gctacattta cctgacccca ataagtttgg ttttcctgac acctcatttt 19080
ataatccaga tacacagcgg ctggtttggg cctgtgtagg tgttgaggta ggctgacacc 19140
tcattttata atccagatac acagcggctg gtttgggcct gtgtaggtgt tgaggtaggt 19200
cgtggtcagc cattaggtgt gggcattagt ggcggctggt ttgggcctgt gtaggtgttg 19260
aggtaggtcg tggtcagcca ttaggtgtgg gcattagtgg ccatccttta ttaaataaat 19320
tggatgacac agggtcgtgg tcagccatta ggtgtgggca ttagtggcca tcctttatta 19380
aataaattgg atgacacaga aacgtatagt gcttcgtaag caacgtaagg tgggccatcc 19440
tttattaaat aaattggatg acacagaaac gtatagtgct tcgtaagcaa cgtaaggtgt 19500
ggataataga gaatgtatat ctatggatta caaaacgtat agtgcttcgt aagcaacgta 19560
aggtgtggat aatagagaat gtatatctat ggattacaaa caaacacaat tgtgtttaat 19620
tggttgcaaa cctggataat agagaatgta tatctatgga ttacaaacaa acacaattgt 19680
gtttaattgg ttgcaaacca cctatagggg aacactgggg caaaggatcc ccaacaaaca 19740
caattgtgtt taattggttg caaaccacct ataggggaac actggggcaa aggatcccca 19800
tgtaccaatg ttgcagtaaa tccaggtgat tgccacctat aggggaacac tggggcaaag 19860
gatccccatg taccaatgtt gcagtaaatc caggtgattg tccaccatta gagttaataa 19920
acacagttat tccatgtacc aatgttgcag taaatccagg tgattgtcca ccattagagt 19980
taataaacac agttattcag gatggtgata tggttgatac tggctttggt gcgtccacca 20040
ttagagttaa taaacacagt tattcaggat ggtgatatgg ttgatactgg ctttggtgct 20100
atggacttta ctacattaca ggctaacaaa agggatggtg atatggttga tactggcttt 20160
ggtgctatgg actttactac attacaggct aacaaaagtg aagttccact ggatatttgt 20220
acatctattt gcgctatgga ctttactaca ttacaggcta acaaaagtga agttccactg 20280
gatatttgta catctatttg caaatatcca gattatatta aaatggtgtc aggtgaagtt 20340
ccactggata tttgtacatc tatttgcaaa tatccagatt atattaaaat ggtgtcagaa 20400
ccatatggcg acagcttatt tttttattta cggcaaatat ccagattata ttaaaatggt 20460
gtcagaacca tatggcgaca gcttattttt ttatttacga agggaacaaa tgtttgttag 20520
acatttattt aaccagatta tattaaaatg gtgtcagaac catatggcga cagcttattt 20580
ttttatttac gaagggaaca aatgtttgtt agacatttat ttaatagggc tgccatatgg 20640
cgacagctta tttttttatt tacgaaggga acaaatgttt gttagacatt tatttaatag 20700
ggctggtact gttggtgaaa atgtaccaga cggaagggaa caaatgtttg ttagacattt 20760
atttaatagg gctggtactg ttggtgaaaa tgtaccagac gatttataca ttaaaggctc 20820
tgggtctact gcatagggct ggtactgttg gtgaaaatgt accagacgat ttatacatta 20880
aaggctctgg gtctactgca aatttagcca gttcaaatta ttttcctaca ccgacgattt 20940
atacattaaa ggctctgggt ctactgcaaa tttagccagt tcaaattatt ttcctacacc 21000
tagtggttct atggttacct ctgcgtacca aagcaaattt agccagttca aattattttc 21060
ctacacctag tggttctatg gttacctctg cgtaccaaat attcaataaa ccttattggt 21120
tacaacgagc acttttccta cacctagtgg ttctatggtt acctctgcgt accaaatatt 21180
caataaacct tattggttac aacgagcaca gggccacaat aatggcctag tggttctatg 21240
gttacctctg cgtaccaaat attcaataaa ccttattggt tacaacgagc acagggccac 21300
aataatggca tttgttgggg taacctattc aataaacctt attggttaca acgagcacag 21360
ggccacaata atggcatttg ttggggtaac caactatttg ttactgttgt tgatactaca 21420
cgcagagggc cacaataatg gcatttgttg gggtaaccaa ctatttgtta ctgttgttga 21480
tactacacgc agtacaaata tgtcattatg tgctgccata tctacccaac tatttgttac 21540
tgttgttgat actacacgca gtacaaatat gtcattatgt gctgccatat ctacttcaga 21600
aactacatat aaaaatacta actttgtaca aatatgtcat tatgtgctgc catatctact 21660
tcagaaacta catataaaaa tactaacttt aaggagtacc tacgacatgg ggaggaatat 21720
gatttcttca gaaactacat ataaaaatac taactttaag gagtacctac gacatgggga 21780
ggaatatgat ttacagttta tttttcaact gtgcaaaata accttggagt acctacgaca 21840
tggggaggaa tatgatttac agtttatttt tcaactgtgc aaaataacct taactgcaga 21900
cgttatgaca tacatacatt ctatgtacag tttatttttc aactgtgcaa aataacctta 21960
actgcagacg ttatgacata catacattct atgaattcca ctattttgga ggactggaat 22020
tttggtaact gcagacgtta tgacatacat acattctatg aattccacta ttttggagga 22080
ctggaatttt ggtctacaac ctcccccagg aggcacacta gaagatgaat tccactattt 22140
tggaggactg gaattttggt ctacaacctc ccccaggagg cacactagaa gatacttata 22200
ggtttgtaac atcccaggca attgcggtct acaacctccc ccaggaggca cactagaaga 22260
tacttatagg tttgtaacat cccaggcaat tgcttgtcaa aaacatacac ctccagcacc 22320
taaaggatac ttataggttt gtaacatccc aggcaattgc ttgtcaaaaa catacacctc 22380
cagcacctaa agaagatccc cttaaaaaat acactttttg ggaaggcttg tcaaaaacat 22440
acacctccag cacctaaaga agatcccctt aaaaaataca ctttttggga agtaaattta 22500
aaggaaaagt tttctgcaga cctaggaaga tccccttaaa aaatacactt tttgggaagt 22560
aaatttaaag gaaaagtttt ctgcagacct agatcagttt cctttaggac gcaaattttt 22620
actacacttt ttgggaagta aatttaaagg aaaagttttc tgcagaccta gatcagtttc 22680
ctttaggacg caaattttta ctacaagcag gattgaaggc cgtaaattta aaggaaaagt 22740
tttctgcaga cctagatcag tttcctttag gacgcaaatt tttactacaa gcaggattga 22800
aggccaaacc aaaatttaca tatcagtttc ctttaggacg caaattttta ctacaagcag 22860
gattgaaggc caaaccaaaa tttacattag gaaaacgaaa agctacaccc accacctcat 22920
cgcaggattg aaggccaaac caaaatttac attaggaaaa cgaaaagcta cacccaccac 22980
ctcatctacc tctacaactg ctaaacgcaa aaaacgtaag ctaggaaaac gaaaagctac 23040
acccaccacc tcatctacct ctacaactgc taaacgcaaa aaacgtaagc tgtaagtatt 23100
gtatgtatgt tgaattagtg tctacctcta caactgctaa acgcaaaaaa cgtaagctgt 23160
aagtattgta tgtatgttga attagtgttg tttgttgtgt atatgtttgt atgtgcttgt 23220
agctgtaagt attgtatgta tgttgaatta gtgttgtttg ttgtgtatat gtttgtatgt 23280
gcttgtatgt gcttgtaaat attaagttgt atgtgtgttt gttgtttgtt gtgtatatgt 23340
ttgtatgtgc ttgtatgtgc ttgtaaatat taagttgtat gtgtgtttgt atgtatggta 23400
taataaacac gtgtgtatgt gatgtgcttg taaatattaa gttgtatgtg tgtttgtatg 23460
tatggtataa taaacacgtg tgtatgtgtt tttaacgtat tgtgtaacta ttgtgtccgt 23520
atgtatgtat ggtataataa acacgtgtgt atgtgttttt aacgtattgt gtaactattg 23580
tgtccgtaaa cataaataaa cttattgttt caacacctac tgtttttaac gtattgtgta 23640
actattgtgt ccgtaaacat aaataaactt attgtttcaa cacctactaa ttgtgttgtg 23700
gttattcatt gtatataaac tgcaacataa ataaacttat tgtttcaaca cctactaatt 23760
gtgttgtggt tattcattgt atataaacta tatttgctac atcctgtttt tgttttatat 23820
aattgtgttg tggttattca ttgtatataa actatatttg ctacatcctg tttttgtttt 23880
atatatacta tattttgtag cgccagcggc cattttgtag ctatatttgc tacatcctgt 23940
ttttgtttta tatatactat attttgtagc gccagcggcc attttgtagc ttcaaccgaa 24000
ttcggttgcc gtatttttgg cctatatttt gtagcgccag cggccatttt gtagcttcaa 24060
ccgaattcgg ttgccgtatt tttggcacaa aatgtgtttt tttaaatagt tctatgtcag 24120
cagcttcaac cgaattcggt tgccgtattt ttggcacaaa atgtgttttt ttaaatagtt 24180
ctatgtcagc aactatggtt taaacttgta cgtttcctgc tcacaaaatg tgttttttta 24240
aatagttcta tgtcagcaac tatggtttaa acttgtacgt ttcctgcttg cccgtagtgc 24300
caaatccctg ttttcctgac ccagcaacta tggtttaaac ttgtacgttt cctgcttgcc 24360
cgtagtgcca aatccctgtt ttcctgacct gcactgcttg ccaaccattc cattgttttt 24420
tttgcccgta gtgccaaatc cctgttttcc tgacctgcac tgcttgccaa ccattccatt 24480
gttttttaca ctgcactatg tgcaactact gaatcactat gctgcactgc ttgccaacca 24540
ttccattgtt ttttacactg cactatgtgc aactactgaa tcactatgta cattgtgtca 24600
tataaaataa atcactcgta gacactgcac tatgtgcaac tactgaatca ctatgtacat 24660
tgtgtcatat aaaataaatc actcgtagcc aacgccttac ataccgctgt taggcacata 24720
tgtacattgt gtcatataaa ataaatcact cgtagccaac gccttacata ccgctgttag 24780
gcacatattt ttggcttgtt ttaactaacc taattgcata tgcgccaacg ccttacatac 24840
cgctgttagg cacatatttt tggcttgttt taactaacct aattgcatat ttggcataag 24900
gtttaaactt ctaaggccaa ctttttggct tgttttaact aacctaattg catatttggc 24960
ataaggttta aacttctaag gccaactaaa tgtcacccta gttcatacat gaactgtgta 25020
attggcataa ggtttaaact tctaaggcca actaaatgtc accctagttc atacatgaac 25080
tgtgtaaagg ttagtcatac attgttcatt tgtaaaactg caatgtcacc ctagttcata 25140
catgaactgt gtaaaggtta gtcatacatt gttcatttgt aaaactgcac atgggtgtgt 25200
gcaaaccgtt ttgggttaca c 25221
<210> 2
<211> 27751
<212> DNA
<213> Artificial Sequence
<220>
<223> 探针型别HPV18
<400> 2
attaatactt ttaacaattg tagtatataa aaaagggagt aaccgaaaac ggtcgggacc 60
gaaaacggtg tatataaaag atgtgagaaa cacaccacaa agggagtaac cgaaaacggt 120
cgggaccgaa aacggtgtat ataaaagatg tgagaaacac accacaatac tatggcgcgc 180
tttgaggatc caacacggcg ccacaatact atggcgcgct ttgaggatcc aacacggcga 240
ccctacaagc tacctgatct gtgcacggaa ctgaacactt cactgcaaga catagaaata 300
cggcgaccct acaagctacc tgatctgtgc acggaactga acacttcact gcaagacata 360
gaaataacct gtgtatattg caagacagta ttggaactta cgaccctaca agctacctga 420
tctgtgcacg gaactgaaca cttcactgca agacatagaa ataacctgtg tatattgcaa 480
gacagtattg gaaccggaac tgaacacttc actgcaagac atagaaataa cctgtgtata 540
ttgcaagaca gtattggaac ttacagaggt atttgaattt gcatttaaag atttcctgtg 600
tatattgcaa gacagtattg gaacttacag aggtatttga atttgcattt aaagatttat 660
ttgtggtgta tagagacagt ataccgccgt atgccctgtg tatattgcaa gacagtattg 720
gaacttacag aggtatttga atttgcattt aaagatttat ttgtggtgta tagagacagt 780
ataccgccgt atgcacagag gtatttgaat ttgcatttaa agatttattt gtggtgtata 840
gagacagtat accgccgtat gccgtacata aatgtataga tttttattct agaatttgtg 900
gtgtatagag acagtatacc gccgtatgcc gtacataaat gtatagattt ttattctaga 960
attagagaat taagacatta ttcagactct gtgttggtgt atagagacag tataccgccg 1020
tatgccgtac ataaatgtat agatttttat tctagaatta gagaattaag acattattca 1080
gactctgtgt atgggccgta cataaatgta tagattttta ttctagaatt agagaattaa 1140
gacattattc agactctgtg tatggagaca cattggaaaa actaactaac actgtgccat 1200
aaatgtatag atttttattc tagaattaga gaattaagac attattcaga ctctgtgtat 1260
ggagacacat tggaaaaact aactaaagag aattaagaca ttattcagac tctgtgtatg 1320
gagacacatt ggaaaaacta actaacactg ggttatacaa tttattaata aggtgcctgc 1380
ggtgccagag aattaagaca ttattcagac tctgtgtatg gagacacatt ggaaaaacta 1440
actaacactg ggttatacaa tttattaata aggtgcctgc ggtgccgggt tatacaattt 1500
attaataagg tgcctgcggt gccagaaacc gttgaatcca gcagaaaaac ttagacacct 1560
taatgaaaaa cgacgatttc acaacaaatt tattaataag gtgcctgcgg tgccagaaac 1620
cgttgaatcc agcagaaaaa cttagacacc ttaatgaaaa acgacgattt cacaacatag 1680
cgtgccagaa accgttgaat ccagcagaaa aacttagaca ccttaatgaa aaacgacgat 1740
ttcacaacat agctgggcac tatagaggcc agtgccattc gagacacctt aatgaaaaac 1800
gacgatttca caacatagct gggcactata gaggccagtg ccattcgtgc tgcaaccgag 1860
cacgacagga acgactccaa cagctgggca ctatagaggc cagtgccatt cgtgctgcaa 1920
ccgagcacga caggaacgac tccaacgacg cagagaaaca caagtataat attaagtcgt 1980
acgacgcaga gaaacacaag tataatatta agtcgtaatg gacctaaggc aacattgcaa 2040
gacattgtat tgcatttaga gccccaaaat gaaattccgg ttgcatggac ctaaggcaac 2100
attgcaagac attgtattgc atttagagcc ccaaaatgaa attccggttg accttctatg 2160
tcacgagcaa ttaagcgact cgtattgcat ttagagcccc aaaatgaaat tccggttgac 2220
cttctatgtc acgagcaatt aagcgactca gaggaagaaa acgatgaaat agatggagtt 2280
attgaccttc tatgtcacga gcaattaagc gactcagagg aagaaaacga tgaaatagat 2340
ggagttaatc atcaacattt accagcccga cgagccgaac ccagaggaag aaaacgatga 2400
aatagatgga gttaatcatc aacatttacc agcccgacga gccgaaccac aacgtcacac 2460
aatgttgtgt atgtgttgta atcatcaaca tttaccagcc cgacgagccg aaccacaacg 2520
tcacacaatg ttgtgtatgt gttgtaagtg tgaagccaga attgagctag tagtagaaag 2580
cccacaacgt cacacaatgt tgtgtatgtg ttgtaagtgt gaagccagaa ttgagctagt 2640
agtagaaagc tcagcagacg accttcgagc attccagcag cagtgtgaag ccagaattga 2700
gctagtagta gaaagctcag cagacgacct tcgagcattc cagcagctgt ttctgaacac 2760
cctgtccttt gtgtgtccgt gctcagcaga cgaccttcga gcattccagc agctgtttct 2820
gaacaccctg tcctttgtgt gtccgtggtg tgcatcccag cagtaagcaa caatggctga 2880
ttgtttctga acaccctgtc ctttgtgtgt ccgtggtgtg catcccagca gtaagcaaca 2940
atggctgatc cagaaggtac agacggggag ggcacgggtt gcaccctgtc ctttgtgtgt 3000
ccgtggtgtg catcccagca gtaagcaaca atggctgatc cagaaggtac agacggggag 3060
ggcacgggtt gggtgtgcat cccagcagta agcaacaatg gctgatccag aaggtacaga 3120
cggggagggc acgggttgta acggctggtt ttatgtacaa gctattgtag accagaaggt 3180
acagacgggg agggcacggg ttgtaacggc tggttttatg tacaagctat tgtagacaaa 3240
aaaacaggag atgtaatatc agatgacgag gccagaaggt acagacgggg agggcacggg 3300
ttgtaacggc tggttttatg tacaagctat tgtagacaaa aaaacaggag atgtaatatc 3360
agatgacgag ggtaacggct ggttttatgt acaagctatt gtagacaaaa aaacaggaga 3420
tgtaatatca gatgacgagg acgaaacgta aacagacaca gggtcggata tacaaaaaaa 3480
caggagatgt aatatcagat gacgaggacg aaacgtaaac agacacaggg tcggatatgg 3540
tagattttat tgatacacaa ggaacatttt gcgaggacga aacgtaaaca gacacagggt 3600
cggatatggt agattttatt gatacacaag gaacattttg tgaacaggca gagctagaga 3660
cagcacaggc agaggacgaa acgtaaacag acacagggtc ggatatggta gattttattg 3720
atacacaagg aacattttgt gaacaggcag agctagagac agcacaggca ttggtagatt 3780
ttattgatac acaaggaaca ttttgtgaac aggcagagct agagacagca caggcattgt 3840
tcccgtagca ggaggtccac aatgcgtaac aggtagattt tattgataca caaggaacat 3900
tttgtgaaca ggcagagcta gagacagcac aggcattgtt cccgtagcag gaggtccaca 3960
atgcgtaaca gtgaacaggc agagctagag acagcacagg cattgttccc gtagcaggag 4020
gtccacaatg cgtaacaagt gttgcatgtt ttaaaacgaa agtttgcagg gtgaacaggc 4080
agagctagag acagcacagg cattgttccc gtagcaggag gtccacaatg cgtaacaagt 4140
gttgcatgtt ttaaaacgaa agtttgcagg gttcccgtag caggaggtcc acaatgcgta 4200
acaagtgttg catgttttaa aacgaaagtt tgcaggaggc agcacagaaa acagtccatt 4260
aggggagcgg gttcccgtag caggaggtcc acaatgcgta acaagtgttg catgttttaa 4320
aacgaaagtt tgcaggaggc agcacagaaa acagtccatt aggggagcgg caagtgttgc 4380
atgttttaaa acgaaagttt gcaggaggca gcacagaaaa cagtccatta ggggagcggc 4440
tggaggtgga tacagagtta agtccacggt caagtgttgc atgttttaaa acgaaagttt 4500
gcaggaggca gcacagaaaa cagtccatta ggggagcggc tggaggtgga tacagagtta 4560
agtccacggt ggaggcagca cagaaaacag tccattaggg gagcggctgg aggtggatac 4620
agagttaagt ccacggttac aagaaatatc tttaaatagt gggcagaaaa ggaggcagca 4680
cagaaaacag tccattaggg gagcggctgg aggtggatac agagttaagt ccacggttac 4740
aagaaatatc tttaaatagt gggcagaaaa ggctggaggt ggatacagag ttaagtccac 4800
ggttacaaga aatatcttta aatagtgggc agaaaaaggc aaaaaggcgg ctgtttacaa 4860
tatcagatag gtggatacag agttaagtcc acggttacaa gaaatatctt taaatagtgg 4920
gcagaaaaag gcaaaaaggc ggctgtttac aatatcagat agacaagaaa tatctttaaa 4980
tagtgggcag aaaaaggcaa aaaggcggct gtttacaata tcagatagtg gctatggctg 5040
ttctgaagtg gaagcacaag aaatatcttt aaatagtggg cagaaaaagg caaaaaggcg 5100
gctgtttaca atatcagata gtggctatgg ctgttctgaa gtggaagcaa cacagggcaa 5160
aaaggcggct gtttacaata tcagatagtg gctatggctg ttctgaagtg gaagcaacac 5220
agattcaggt aactacaaat ggcgaacatg gcggcgtggc tatggctgtt ctgaagtgga 5280
agcaacacag attcaggtaa ctacaaatgg cgaacatggc ggcaatgtat gtagtggcgg 5340
cagtacggag gctatagatt caggtaacta caaatggcga acatggcggc aatgtatgta 5400
gtggcggcag tacggaggct atagacaacg ggggcacaga gggcaacaac agcaggcaat 5460
gtatgtagtg gcggcagtac ggaggctata gacaacgggg gcacagaggg caacaacagc 5520
agtgtagacg gtacaagtga caatagcaat atagatagac aacgggggca cagagggcaa 5580
caacagcagt gtagacggta caagtgacaa tagcaatata gaaaatgtaa atccacaatg 5640
taccatagca caattgtgta gacggtacaa gtgacaatag caatatagaa aatgtaaatc 5700
cacaatgtac catagcacaa ttaaaagact tgttaaaagt aaacaataaa caaggaatgt 5760
aaatccacaa tgtaccatag cacaattaaa agacttgtta aaagtaaaca ataaacaagg 5820
agctatgtta gcagtattta aagacacata tgggctaaaa gacttgttaa aagtaaacaa 5880
taaacaagga gctatgttag cagtatttaa agacacatat gggctatcat ttacagattt 5940
agttagaaat tttaagagct atgttagcag tatttaaaga cacatatggg ctatcattta 6000
cagatttagt tagaaatttt aaaagtgata aaaccacgtg tacagattgg gttacgggct 6060
atcatttaca gatttagtta gaaattttaa aagtgataaa accacgtgta cagattgggt 6120
tacagctata tttggagtaa acccaacaat agcagttaca gatttagtta gaaattttaa 6180
aagtgataaa accacgtgta cagattgggt tacagctata tttggagtaa acccaacaat 6240
agcagaagga tttaaaaagt gataaaacca cgtgtacaga ttgggttaca gctatatttg 6300
gagtaaaccc aacaatagca gaaggattta aaacactaat acagccattt atattcagct 6360
atatttggag taaacccaac aatagcagaa ggatttaaaa cactaataca gccatttata 6420
ttatcgtacc atattcaatg tctagactgt aaatggaagg atttaaaaca ctaatacagc 6480
catttatatt atcgtaccat attcaatgtc tagactgtaa atggggagta ttaatattag 6540
ccctgttgcg ttacatatcg taccatattc aatgtctaga ctgtaaatgg ggagtattaa 6600
tattagccct gttgcgttac aaatgtggta agagtagact aacagttgct aaagggggga 6660
gtattaatat tagccctgtt gcgttacaaa tgtggtaaga gtagactaac agttgctaaa 6720
ggtttaagta cgttgttaca cgtacctgaa acttgaatgt ggtaagagta gactaacagt 6780
tgctaaaggt ttaagtacgt tgttacacgt acctgaaact tgtatgttaa ttcaaccacc 6840
aaaattgcga agtagggttt aagtacgttg ttacacgtac ctgaaacttg tatgttaatt 6900
caaccaccaa aattgcgaag tagtgttgca gcactatatt ggtatagaac aggaacgttg 6960
ttacacgtac ctgaaacttg tatgttaatt caaccaccaa aattgcgaag tagtgttgca 7020
gcactatatt ggtatagaac aggaatatca aatatgtatg ttaattcaac caccaaaatt 7080
gcgaagtagt gttgcagcac tatattggta tagaacagga atatcaaata ttagtgaagt 7140
aatgggagac acaccttcaa ccaccaaaat tgcgaagtag tgttgcagca ctatattggt 7200
atagaacagg aatatcaaat attagtgaag taatgggaga cacacctgag tggatccaaa 7260
attgcgaagt agtgttgcag cactatattg gtatagaaca ggaatatcaa atattagtga 7320
agtaatggga gacacacctg agtggataca aagacgtgtt gcagcactat attggtatag 7380
aacaggaata tcaaatatta gtgaagtaat gggagacaca cctgagtgga tacaaagact 7440
tactattata caacagcact atattggtat agaacaggaa tatcaaatat tagtgaagta 7500
atgggagaca cacctgagtg gatacaaaga cttactatta tacaacatgg gcactatatt 7560
ggtatagaac aggaatatca aatattagtg aagtaatggg agacacacct gagtggatac 7620
aaagacttac tattatacaa catggaatag tggtatagaa caggaatatc aaatattagt 7680
gaagtaatgg gagacacacc tgagtggata caaagactta ctattataca acatggaata 7740
gatgatagct atcaaatatt agtgaagtaa tgggagacac acctgagtgg atacaaagac 7800
ttactattat acaacatgga atagatgata gcaattttga tttgtcagat tagtgaagta 7860
atgggagaca cacctgagtg gatacaaaga cttactatta tacaacatgg aatagatgat 7920
agcaattttg atttgtcaga aatggtacac tgagtggata caaagactta ctattataca 7980
acatggaata gatgatagca attttgattt gtcagaaatg gtacaatggg catttgataa 8040
tgagctgact acaaagactt actattatac aacatggaat agatgatagc aattttgatt 8100
tgtcagaaat ggtacaatgg gcatttgata atgagctgac agatgaaagc atggaataga 8160
tgatagcaat tttgatttgt cagaaatggt acaatgggca tttgataatg agctgacaga 8220
tgaaagcgat atggcatttg aatcgtactg aaatggtaca atgggcattt gataatgagc 8280
tgacagatga aagcgatatg gcatttgaat cgtacttatt agcagacagc aacagcacgt 8340
aagctgcctc agatgaaagc gatatggcat ttgaatcgta cttattagca gacagcaaca 8400
gcacgtaagc tgccttttta aaaagcaatt gccaagctaa atatttaaag cgatatggca 8460
tttgaatcgt acttattagc agacagcaac agcacgtaag ctgccttttt aaaaagcaat 8520
tgccaagcta aatatttaaa agattgtgct attagcagac agcaacagca cgtaagctgc 8580
ctttttaaaa agcaattgcc aagctaaata tttaaaagat tgtgccacaa tgtgcaaaca 8640
ttataggcgt ttaaaaagca attgccaagc taaatattta aaagattgtg ccacaatgtg 8700
caaacattat aggcgagccc aaaaacgaca aatgaatatg tcacagtggg attgtgccac 8760
aatgtgcaaa cattataggc gagcccaaaa acgacaaatg aatatgtcac agtggatacg 8820
atttagatgt tcaaaaatag atgaaggggg agcccaaaaa cgacaaatga atatgtcaca 8880
gtggatacga tttagatgtt caaaaataga tgaaggggga gattggagac caatagtgca 8940
attcctgcgg gatacgattt agatgttcaa aaatagatga agggggagat tggagaccaa 9000
tagtgcaatt cctgcgatac caacaaatag agtttataac atttttaggg ggggagattg 9060
gagaccaata gtgcaattcc tgcgatacca acaaatagag tttataacat ttttaggagc 9120
cttaaaatca tttttaaaag gaacccccag cgataccaac aaatagagtt tataacattt 9180
ttaggagcct taaaatcatt tttaaaagga acccccaaaa aaaattgttt agtattttgt 9240
ggaccagcag gagccttaaa atcattttta aaaggaaccc ccaaaaaaaa ttgtttagta 9300
ttttgtggac cagcaaatac aggaaaatca tattttggaa tgagttttaa aaaaattgtt 9360
tagtattttg tggaccagca aatacaggaa aatcatattt tggaatgagt tttatacact 9420
ttatacaagg agcagtaata tcatttgtga tacaggaaaa tcatattttg gaatgagttt 9480
tatacacttt atacaaggag cagtaatatc atttgtgaat tccactagtc atttttggtt 9540
ggaaccgttt acactttata caaggagcag taatatcatt tgtgaattcc actagtcatt 9600
tttggttgga accgttaaca gatactaagg tggccatgtt agatgcgtag tgaattccac 9660
tagtcatttt tggttggaac cgttaacaga tactaaggtg gccatgttag atgcgtaaac 9720
gaccacgtgt tggacatact ttgatacctt aacagatact aaggtggcca tgttagatgc 9780
gtaaacgacc acgtgttgga catactttga tacctatatg agaacgtagt tagatggcaa 9840
tccaataagg caacgaccac gtgttggaca tactttgata cctatatgag aacgtagtta 9900
gatggcaatc caataagtat tgatagaaag cacaaaccat taatacaaca tatgagaacg 9960
tagttagatg gcaatccaat aagtattgat agaaagcaca aaccattaat acaactaaaa 10020
tgtcctccaa tactactaac cacaaatatg tattgataga aagcacaaac cattaataca 10080
actaaaatgt cctccaatac tactaaccac aaatatacat ccagcaaagg ataatagatg 10140
gccatatttc taaaatgtcc tccaatacta ctaaccacaa atatacatcc agcaaaggat 10200
aatagatggc catatttaga aagtagaata acagtatttg aatttccaat acatccagca 10260
aaggataata gatggccata tttagaaagt agaataacag tatttgaatt tccaacgtaa 10320
tttccatttg ataaaaatgg caatccagtg caaaggataa tagatggcca tatttagaaa 10380
gtagaataac agtatttgaa tttccaacgt aatttccatt tgataaaaat ggcaatccag 10440
tatatgaaat agaaagtaga ataacagtat ttgaatttcc aacgtaattt ccatttgata 10500
aaaatggcaa tccagtatat gaaataaatg acaaaaattg gaaatgtttg tagaataaca 10560
gtatttgaat ttccaacgta atttccattt gataaaaatg gcaatccagt atatgaaata 10620
aatgacaaaa attggaaatg tatttgaatt tccaacgtaa tttccatttg ataaaaatgg 10680
caatccagta tatgaaataa atgacaaaaa ttggaaatgt ttttttgaaa gccatttgat 10740
aaaaatggca atccagtata tgaaataaat gacaaaaatt ggaaatgttt ttttgaaagg 10800
acatggtcca gattagattt gcacgaggaa gaatccagta tatgaaataa atgacaaaaa 10860
ttggaaatgt ttttttgaaa ggacatggtc cagattagat ttgcacgagg aagaggaagc 10920
gtataaatga caaaaattgg aaatgttttt ttgaaaggac atggtccaga ttagatttgc 10980
acgaggaaga ggaagcgtaa gacaccgaag gaaacccttt cggggacatg gtccagatta 11040
gatttgcacg aggaagagga agcgtaagac accgaaggaa accctttcgg aacgtttaag 11100
tgcgttgcag gacaaaatga agcgtaagac accgaaggaa accctttcgg aacgtttaag 11160
tgcgttgcag gacaaaatca tagaccacta tgaaaatgac agtaaagaca tagacagcac 11220
cctttcggaa cgtttaagtg cgttgcagga caaaatcata gaccactatg aaaatgacag 11280
taaagacata gacagccaaa tacagtattg gcaactaaga acgtttaagt gcgttgcagg 11340
acaaaatcat agaccactat gaaaatgaca gtaaagacat agacagccaa atacagtatt 11400
ggcaactaat acgttgggat catagaccac tatgaaaatg acagtaaaga catagacagc 11460
caaatacagt attggcaact aatacgttgg gaaacgtaaa tattctttgc agcaagggga 11520
ccactatgaa aatgacagta aagacataga cagccaaata cagtattggc aactaatacg 11580
ttgggaaacg taaatattct ttgcagcaag ggaacatggc caaatacagt attggcaact 11640
aatacgttgg gaaacgtaaa tattctttgc agcaagggaa catggcatac agacattaaa 11700
ccaccaggtg gtgcctacgt tgggaaacgt aaatattctt tgcagcaagg gaacatggca 11760
tacagacatt aaaccaccag gtggtgccag cctataacat ttcaaaaagt aaagcaggga 11820
acatggcata cagacattaa accaccaggt ggtgccagcc tataacattt caaaaagtaa 11880
agcacataaa gctattgaac tgcaaatggc cctacaccac caggtggtgc cagcctataa 11940
catttcaaaa agtaaagcac ataaagctat tgaactgcaa atggccctac aaggccttgc 12000
acaaagtggt gccagcctat aacatttcaa aaagtaaagc acataaagct attgaactgc 12060
aaatggccct acaaggcctt gcacaaagtg catacaaaac cgagggcaca taaagctatt 12120
gaactgcaaa tggccctaca aggccttgca caaagtgcat acaaaaccga ggattggaca 12180
ctgcaagaca ccgtagagga actatgctat tgaactgcaa atggccctac aaggccttgc 12240
acaaagtgca tacaaaaccg aggattggac actgcaagac accgtagagg aactatggaa 12300
tacagcaagg ccttgcacaa agtgcataca aaaccgagga ttggacactg caagacaccg 12360
tagaggaact atggaataca gaacctactc actgctttaa aaaagggatt ggacactgca 12420
agacaccgta gaggaactat ggaatacaga acctactcac tgctttaaaa aaggtggcca 12480
aacagtacaa gtatattttg atggcctgca agacaccgta gaggaactat ggaatacaga 12540
acctactcac tgctttaaaa aaggtggcca aacagtacaa gtatattttg atggcaacaa 12600
agacatggaa tacagaacct actcactgct ttaaaaaagg tggccaaaca gtacaagtat 12660
attttgatgg caacaaagac aattgtatga cctatgtagc atgggggtgg ccaaacagta 12720
caagtatatt ttgatggcaa caaagacaat tgtatgacct atgtagcatg ggacagtgtg 12780
tattatatga ctgcgtaagg aacatacaaa gacaattgta tgacctatgt agcatgggac 12840
agtgtgtatt atatgactgc gtaaggaaca tgggacaaaa cggctacctg tgtaagtcac 12900
aggggggaca gtgtgtatta tatgactgcg taaggaacat gggacaaaac ggctacctgt 12960
gtaagtcaca ggggattgta ttatgtaaag gaagggtaca acacgtggga caaaacggct 13020
acctgtgtaa gtcacagggg attgtattat gtaaaggaag ggtacaacac gttttatata 13080
gaatttaaaa gtgaatgtga aaaatggatt gtattatgta aaggaagggt acaacacgtt 13140
ttatatagaa tttaaaagtg aatgtgaaaa atatgggaac acaggtacgt gggaagtaca 13200
ttttgttata tagaatttaa aagtgaatgt gaaaaatatg ggaacacagg tacgtgggaa 13260
gtacattttg ggaataatgt aattgattgt aatgactcta tgtgcaattt aaaagtgaat 13320
gtgaaaaata tgggaacaca ggtacgtggg aagtacattt tgggaataat gtaattgatt 13380
gtaatgactc tatgtgcagt accatatggg aacacaggta cgtgggaagt acattttggg 13440
aataatgtaa ttgattgtaa tgactctatg tgcagtacca gtgacgacac ggtatccgct 13500
actctacgtg ggaagtacat tttgggaata atgtaattga ttgtaatgac tctatgtgca 13560
gtaccagtga cgacacggta tccgctactc agcttgttag ggaataatgt aattgattgt 13620
aatgactcta tgtgcagtac cagtgacgac acggtatccg ctactcagct tgttaaacag 13680
ctacagcaca ccccctcact gcagtaccag tgacgacacg gtatccgcta ctcagcttgt 13740
taaacagcta cagcacaccc cctcaccgta ttccagcacc gtgtccgtgg gcaccgcaat 13800
cagcttgtta aacagctaca gcacaccccc tcaccgtatt ccagcaccgt gtccgtgggc 13860
accgcaaaga cctacggcca gacgtcggct gctacacgac cgtattccag caccgtgtcc 13920
gtgggcaccg caaagaccta cggccagacg tcggctgcta cacgacctgg acactgtgga 13980
ctcgcggaga agcagcatta agacctacgg ccagacgtcg gctgctacac gacctggaca 14040
ctgtggactc gcggagaagc agcattgtgg acctgtcaac ccacttctcg gtgcagctac 14100
ctggacactg tggactcgcg gagaagcagc attgtggacc tgtcaaccca cttctcggtg 14160
cagctacacc tacaggcaac aacaaaagac ggaaactctt gtggacctgt caacccactt 14220
ctcggtgcag ctacacctac aggcaacaac aaaagacgga aactctgtag tggtaacact 14280
acgcctataa tacatttaaa cacctacagg caacaacaaa agacggaaac tctgtagtgg 14340
taacactacg cctataatac atttaaaagg tgacagaaac agtttaaaat gtttacggtt 14400
gtagtggtaa cactacgcct ataatacatt taaaaggtga cagaaacagt ttaaaatgtt 14460
tacggtacag attgcgaaaa catagcgacc actatagaga ggtgacagaa acagtttaaa 14520
atgtttacgg tacagattgc gaaaacatag cgaccactat agagatatat catccacctg 14580
gcattggaca ggtgcaggcg atatatcatc cacctggcat tggacaggtg caggcaatga 14640
aaaaacagga atactgactg taacatacca tagtgaaaca caaagaacaa aatttttaag 14700
gcaatgaaaa aacaggaata ctgactgtaa cataccatag tgaaacacaa agaacaaaat 14760
ttttaaatac tgttgcaatt ccagatagtg tacaaatata ccatagtgaa acacaaagaa 14820
caaaattttt aaatactgtt gcaattccag atagtgtaca aatattggtg ggatacatga 14880
caatgtaata catcgtatga atactgttgc aattccagat agtgtacaaa tattggtggg 14940
atacatgaca atgtaataca tcgtatgtag taccaatatg ttatcactta tttttttatt 15000
tggtgggata catgacaatg taatacatcg tatgtagtac caatatgtta tcacttattt 15060
ttttattttg cttttgtgtc gtaatgtatg tgtgctgccg tagtaccaat atgttatcac 15120
ttattttttt attttgcttt tgtgtcgtaa tgtatgtgtg ctgccatgtc ccgcttttgc 15180
catctgtctg tatgtgtgct tttgcttttg tgtcgtaatg tatgtgtgct gccatgtccc 15240
gcttttgcca tctgtctgta tgtgtgcgtc gtaatgggta ttggtatttg tgtatattgc 15300
atgtcccgct tttgccatct gtctgtatgt gtgcgtcgta atgggtattg gtatttgtgt 15360
atattgtggt aataacgtcc cctgccacag cattcacagg cgtcgtaatg ggtattggta 15420
tttgtgtata ttgtggtaat aacgtcccct gccacagcat tcacagtata tgtattttgt 15480
tttttattgc ccatgttacg tggtaataac gtcccctgcc acagcattca cagtatatgt 15540
attttgtttt ttattgccca tgttactatt gcatataccg tatatattgt ctttacagtg 15600
tatatgtatt ttgtttttta ttgcccatgt tactattgca tataccgtat atattgtctt 15660
tacagtaatt gtataggttg ttttatacag tgtattgtac tattgcatat accgtatata 15720
ttgtctttac agtaattgta taggttgttt tatacagtgt attgtacatt gtatattttg 15780
ttttatacct tttcgtattt tgtataggtt gttttataca gtgtattgta cattgtatat 15840
tttgttttat accttttcgt atttttgtat ttttgtaata aaagtatggt atcccaccgt 15900
tttgtatttt tgtaataaaa gtatggtatc ccaccgtgcc gcacgacgca aacgggcttc 15960
ggtaactgac ttatataaaa catgtaaaca atctggtacc cgtgccgcac gacgcaaacg 16020
ggcttcggta actgacttat ataaaacatg taaacaatct ggtacatgtc cacctgatgt 16080
tgttcctaag gtggagggcg ccgcacgacg caaacgggct tcggtaactg acttatataa 16140
aacatgtaaa caatctggta catgtccacc tgatgttgtt cctaaggtgg agctgactta 16200
tataaaacat gtaaacaatc tggtacatgt ccacctgatg ttgttcctaa ggtggagggc 16260
accacgttag cagataaaat attgcaatgg tccatgtcca cctgatgttg ttcctaaggt 16320
ggagggcacc acgttagcag ataaaatatt gcaatggtca agccttggta tatttttggg 16380
tggacttggc atgcaccacg ttagcagata aaatattgca atggtcaagc cttggtatat 16440
ttttgggtgg acttggcata ggtactggca gtggtacagg gggtcgtaca ggcaagcctt 16500
ggtatatttt tgggtggact tggcataggt actggcagtg gtacaggggg tcgtacaggg 16560
tacattccat tgggtgggcg ttccaataca gttaggtact ggcagtggta cagggggtcg 16620
tacagggtac attccattgg gtgggcgttc caatacagtg gtggatgttg gtcctacacg 16680
tcccccagtg gtgtacattc cattgggtgg gcgttccaat acagtggtgg atgttggtcc 16740
tacacgtccc ccagtggtta ttgaacctgt gggccccaca gacccatcta tttggtggat 16800
gttggtccta cacgtccccc agtggttatt gaacctgtgg gccccacaga cccatctatt 16860
gttacattaa tagaggactc cagtgtggtt actattgaac ctgtgggccc cacagaccca 16920
tctattgtta cattaataga ggactccagt gtggttacat caggtgcacc taggcctacg 16980
tttactggca cgttgttaca ttaatagagg actccagtgt ggttacatca ggtgcaccta 17040
ggcctacgtt tactggcacg tctgggtttg atataacatc tgcgggtaca accatcaggt 17100
gcacctaggc ctacgtttac tggcacgtct gggtttgata taacatctgc gggtacaact 17160
acacctgcgg ttttggatat cacaccttcg tccgtctggg tttgatataa catctgcggg 17220
tacaactaca cctgcggttt tggatatcac accttcgtct acctctgtgt ctatttccac 17280
aaccaatttt acctacacct gcggttttgg atatcacacc ttcgtctacc tctgtgtcta 17340
tttccacaac caattttacc aatcctgcat tttctgatcc gtccattatt gactacctct 17400
gtgtctattt ccacaaccaa ttttaccaat cctgcatttt ctgatccgtc cattattgaa 17460
gttccacaaa ctggggaggt ggcaggtaat gtccaatcct gcattttctg atccgtccat 17520
tattgaagtt ccacaaactg gggaggtggc aggtaatgta tttgttggta cccctacatc 17580
tggaacacat ggaagttcca caaactgggg aggtggcagg taatgtattt gttggtaccc 17640
ctacatctgg aacacatggg tatgaggaaa tacctttaca aacatttgct tcccacaaac 17700
tggggaggtg gcaggtaatg tatttgttgg tacccctaca tctggaacac atgggtatga 17760
ggaaatacct ttacaaacat ttgctcaaac tggggaggtg gcaggtaatg tatttgttgg 17820
tacccctaca tctggaacac atgggtatga ggaaatacct ttacaaacat ttgcttcttc 17880
tggttgttgg tacccctaca tctggaacac atgggtatga ggaaatacct ttacaaacat 17940
ttgcttcttc tggtacgggg gaggaaccca ttagtagtac cccaaatacc tttacaaaca 18000
tttgcttctt ctggtacggg ggaggaaccc attagtagta ccccattgcc tactgtgcgg 18060
cgtgtagcag gtccccgcct ttattctggt acgggggagg aacccattag tagtacccca 18120
ttgcctactg tgcggcgtgt agcaggtccc cgcctttaca gtagggccta ccaacaagtg 18180
tcaaccccat tgcctactgt gcggcgtgta gcaggtcccc gcctttacag tagggcctac 18240
caacaagtgt cagtggctaa ccctgagttt cttacacgtc catccccgcc tttacagtag 18300
ggcctaccaa caagtgtcag tggctaaccc tgagtttctt acacgtccat cctctttaat 18360
tacatatgac aacccggcct ttgcagtggc taaccctgag tttcttacac gtccatcctc 18420
tttaattaca tatgacaacc cggcctttga gcctgtggac actacattaa catttgatcc 18480
tcgcctcttt aattacatat gacaacccgg cctttgagcc tgtggacact acattaacat 18540
ttgatcctcg tagtgatgtt cctgattcag attttatgga tatgcctgtg gacactacat 18600
taacatttga tcctcgtagt gatgttcctg attcagattt tatggatatt atccgtctac 18660
ataggcctgc tttaacatcc aggcgtagtg atgttcctga ttcagatttt atggatatta 18720
tccgtctaca taggcctgct ttaacatcca ggcgtgggac tgttcgcttt agtagattag 18780
gtcttatccg tctacatagg cctgctttaa catccaggcg tgggactgtt cgctttagta 18840
gattaggtca acgggcaact atgtttaccc gcagcggtac acaggcgtgg gactgttcgc 18900
tttagtagat taggtcaacg ggcaactatg tttacccgca gcggtacaca aataggtgct 18960
agggttcact tttatcatga tatcaacggg caactatgtt tacccgcagc ggtacacaaa 19020
taggtgctag ggttcacttt tatcatgata taagtcctat tgcaccttcc ccagaatata 19080
ttgcaaatag gtgctagggt tcacttttat catgatataa gtcctattgc accttcccca 19140
gaatatattg aactgcagcc tttagtatct gccacggagg acagtcctat tgcaccttcc 19200
ccagaatata ttgaactgca gcctttagta tctgccacgg aggacaatga cttgtttgat 19260
atatcgtaag atgacatgga cccactgcag cctttagtat ctgccacgga ggacaatgac 19320
ttgtttgata tatcgtaaga tgacatggac cctgcagtgc ctgtaccatc gcgttctact 19380
accatgactt gtttgatata tcgtaagatg acatggaccc tgcagtgcct gtaccatcgc 19440
gttctactac ctcctttgca ttttttaaat attcgcccac tatgaccctg cagtgcctgt 19500
accatcgcgt tctactacct cctttgcatt ttttaaatat tcgcccacta tatcttctgc 19560
ctcttcctat agtaatgtaa cggcctcctt tgcatttttt aaatattcgc ccactatatc 19620
ttctgcctct tcctatagta atgtaacggt ccctttaacc tcctcttggg atgtgcctgt 19680
ataatcttct gcctcttcct atagtaatgt aacggtccct ttaacctcct cttgggatgt 19740
gcctgtatac acgggtcctg atattacatt accatctact acctcccttt aacctcctct 19800
tgggatgtgc ctgtatacac gggtcctgat attacattac catctactac ctctgtatgg 19860
cccattgtat cacccacggc cccacacggg tcctgatatt acattaccat ctactacctc 19920
tgtatggccc attgtatcac ccacggcccc tgcctctaca cagtatattg gtatacatgg 19980
taccctctgt atggcccatt gtatcaccca cggcccctgc ctctacacag tatattggta 20040
tacatggtac acattattat ttgtggccat tatattattt tatcacatta ttatttgtgg 20100
ccattatatt attttattcc taagaaacgt aaacgtgttc cctatttttt tgcagatggc 20160
tttgtggcgg cctagtgaca atattcctaa gaaacgtaaa cgtgttccct atttttttgc 20220
agatggcttt gtggcggcct agtgacaata ccgtatatct tccacctcct tctgtggcaa 20280
gagaagaaac gtaaacgtgt tccctatttt tttgcagatg gctttgtggc ggcctagtga 20340
caataccgta tatcttccac ctccttctgt ggcaagagtt gtgcagatgg ctttgtggcg 20400
gcctagtgac aataccgtat atcttccacc tccttctgtg gcaagagttg taaataccga 20460
tgattatgtg actcgcacaa gcgatggctt tgtggcggcc tagtgacaat accgtatatc 20520
ttccacctcc ttctgtggca agagttgtaa ataccgatga ttatgtgact cgcacaagca 20580
taaccgtata tcttccacct ccttctgtgg caagagttgt aaataccgat gattatgtga 20640
ctcgcacaag catattttat ccgtatggca gctctagatt attatcttcc acctccttct 20700
gtggcaagag ttgtaaatac cgatgattat gtgactcgca caagcatatt ttatccgtat 20760
ggcagctcta gattattaac tggttgtaaa taccgatgat tatgtgactc gcacaagcat 20820
attttatccg tatggcagct ctagattatt aactgttggt aatccatatt ttagttgtaa 20880
ataccgatga ttatgtgact cgcacaagca tattttatcc gtatggcagc tctagattat 20940
taactgttgg taatccatat tttagggttc ctgagcatat tttatccgta tggcagctct 21000
agattattaa ctgttggtaa tccatatttt agggttcctg caggtggtgg caataagcag 21060
gatattccta aggttaactg ttggtaatcc atattttagg gttcctgcag gtggtggcaa 21120
taagcaggat attcctaagg tttctgcata ccaatataga gtatttaggg tgcgcaggtg 21180
gtggcaataa gcaggatatt cctaaggttt ctgcatacca atatagagta tttagggtgc 21240
agttacctga cccaaataaa tttggtttac ctgggtttct gcataccaat atagagtatt 21300
tagggtgcag ttacctgacc caaataaatt tggtttacct gatactagta tttataatcc 21360
tgaaacacaa cgtcagttac ctgacccaaa taaatttggt ttacctgata ctagtattta 21420
taatcctgaa acacaacgtt tagtgtgggc ctgtgctgga gtggaaattg gccgatacta 21480
gtatttataa tcctgaaaca caacgtttag tgtgggcctg tgctggagtg gaaattggcc 21540
gtggtcagcc tttaggtgtt ggccttagtg ggctagtgtg ggcctgtgct ggagtggaaa 21600
ttggccgtgg tcagccttta ggtgttggcc ttagtgggca tccattttat aataaattag 21660
atgacactga aagcgtggtc agcctttagg tgttggcctt agtgggcatc cattttataa 21720
taaattagat gacactgaaa gttccccgta cgccacgtct aatgtttctg aggcatccat 21780
tttataataa attagatgac actgaaagtt ccccgtacgc cacgtctaat gtttctgagg 21840
acgttaggga caatgtgtct gtagattata agcgttcccc gtacgccacg tctaatgttt 21900
ctgaggacgt tagggacaat gtgtctgtag attataagca gacacagtta tgtattttgg 21960
gctgtgcccc tgcggacgtt agggacaatg tgtctgtaga ttataagcag acacagttat 22020
gtattttggg ctgtgcccct gctattgggg aacactgggc taaaggcact gctcagacac 22080
agttatgtat tttgggctgt gcccctgcta ttggggaaca ctgggctaaa ggcactgctt 22140
gtaaatcgcg tcctttatca cagggcgatt gccgctattg gggaacactg ggctaaaggc 22200
actgcttgta aatcgcgtcc tttatcacag ggcgattgcc cccctttaga acttaaaaac 22260
acagttttgg aagtgtaaat cgcgtccttt atcacagggc gattgccccc ctttagaact 22320
taaaaacaca gttttggaag atggtgatat ggtagatact ggatatggtg ccaccccctt 22380
tagaacttaa aaacacagtt ttggaagatg gtgatatggt agatactgga tatggtgcca 22440
tggactttag tacattgcaa gatactaaat gtggatggtg atatggtaga tactggatat 22500
ggtgccatgg actttagtac attgcaagat actaaatgtg aggtaccatt ggatatttgt 22560
cagtctattt gtatggactt tagtacattg caagatacta aatgtgaggt accattggat 22620
atttgtcagt ctatttgtaa atatcctgat tatttacaaa tgtctgcaga tccgaggtac 22680
cattggatat ttgtcagtct atttgtaaat atcctgatta tttacaaatg tctgcagatc 22740
cttatgggga ttccatgttt ttttgcttac ggccagtcta tttgtaaata tcctgattat 22800
ttacaaatgt ctgcagatcc ttatggggat tccatgtttt tttgcttacg gcgtgagcag 22860
ctttttgcta ggcaatatcc tgattattta caaatgtctg cagatcctta tggggattcc 22920
atgttttttt gcttacggcg tgagcagctt tttgctaggc atttttggaa tagccttatg 22980
gggattccat gtttttttgc ttacggcgtg agcagctttt tgctaggcat ttttggaata 23040
gagcaggtac tatgggtgac actgtgcctc aatggcgtga gcagcttttt gctaggcatt 23100
tttggaatag agcaggtact atgggtgaca ctgtgcctca atccttatat attaaaggca 23160
caggtcgtag tgcgagcagg tactatgggt gacactgtgc ctcaatcctt atatattaaa 23220
ggcacaggtc gtagtgcttc acctggcagc tgtgtgtatt ctccctctcc aagtccttat 23280
atattaaagg cacaggtcgt agtgcttcac ctggcagctg tgtgtattct ccctctccaa 23340
gtggctctat tgttacctct gactcccagt tgtcacctgg cagctgtgtg tattctccct 23400
ctccaagtgg ctctattgtt acctctgact cccagttgtt taataaacca tattggttac 23460
ataaggcaca gggagtggct ctattgttac ctctgactcc cagttgttta ataaaccata 23520
ttggttacat aaggcacagg gtcataacaa tggtgtttgc tggcataatc aatgtttaat 23580
aaaccatatt ggttacataa ggcacagggt cataacaatg gtgtttgctg gcataatcaa 23640
ttatttgtta ctgtggtaga tactaataaa ccatattggt tacataaggc acagggtcat 23700
aacaatggtg tttgctggca taatcaatta tttgttactg tggtagatac cactcgcagt 23760
accggtcata acaatggtgt ttgctggcat aatcaattat ttgttactgt ggtagatacc 23820
actcgcagta ccaatttaac aatatgtgct tctacacagt ctcttatttg ttactgtggt 23880
agataccact cgcagtacca atttaacaat atgtgcttct acacagtctc ctgtacctgg 23940
gcaatatgcg tataccaaat ttaaccaatt taacaatatg tgcttctaca cagtctcctg 24000
tacctgggca atatgcgtat accaaattta agcagtatag cagacatgtt gaggaatatg 24060
attcctgtac ctgggcaata tgcgtatacc aaatttaagc agtatagcag acatgttgag 24120
gaatatgatt tgcagtttat ttttcagttg tgtactatta ctttttaagc agtatagcag 24180
acatgttgag gaatatgatt tgcagtttat ttttcagttg tgtactatta ctttaactgc 24240
agatgttatg tcctatattc ataagcagta tagcagacat gttgaggaat atgatttgca 24300
gtttattttt cagttgtgta ctattacttt aactgcagat gttatgtcct atattcatag 24360
tatgatttgc agtttatttt tcagttgtgt actattactt taactgcaga tgttatgtcc 24420
tatattcata gtatgaatag cagtatttta gaggattgga atgcagttta tttttcagtt 24480
gtgtactatt actttaactg cagatgttat gtcctatatt catagtatga atagcagtat 24540
tttagaggat tggaactttg gttaactgca gatgttatgt cctatattca tagtatgaat 24600
agcagtattt tagaggattg gaactttggt gttccccccc cgccaactac tagtttggtg 24660
gagtatgaat agcagtattt tagaggattg gaactttggt gttccccccc cgccaactac 24720
tagtttggtg gatacatatc gttttgtaca atctgttgct gaatagcagt attttagagg 24780
attggaactt tggtgttccc cccccgccaa ctactagttt ggtggataca tatcgttttg 24840
tacaatctgt tgctattacc ggtgttcccc ccccgccaac tactagtttg gtggatacat 24900
atcgttttgt acaatctgtt gctattacct gtcaaaaggc gtatgcaccg gctgaaaata 24960
gatacatatc gttttgtaca atctgttgct attacctgtc aaaaggcgta tgcaccggct 25020
gaaaataagg atccctatga taagttaaag ttttggaatg acctgtcaaa aggcgtatgc 25080
accggctgaa aataaggatc cctatgataa gttaaagttt tggaatgtgg atttaaagga 25140
aaagttttct ttagacttag aaggatccct atgataagtt aaagttttgg aatgtggatt 25200
taaaggaaaa gttttcttta gacttagatc aatatcccct tggacgtaaa tttttggttc 25260
gtggatttaa aggaaaagtt ttctttagac ttagatcaat atccccttgg acgtaaattt 25320
ttggttcagg ctggattgcg tcgcaagccc accataggcc gatcaatatc cccttggacg 25380
taaatttttg gttcaggctg gattgcgtcg caagcccacc ataggccctc gcaaacgttc 25440
tgctccatct gccactacgt tcaggctgga ttgcgtcgca agcccaccat aggccctcgc 25500
aaacgttctg ctccatctgc cactacgtct tctaaacctg ccaagcgtgt gcgtgtacgt 25560
ggccctcgca aacgttctgc tccatctgcc actacgtctt ctaaacctgc caagcgtgtg 25620
cgtgtacgtg ccaggaagta atatgtgtgt gtgtatatat cgtcttctaa acctgccaag 25680
cgtgtgcgtg tacgtgccag gaagtaatat gtgtgtgtgt atatatatat acatctattg 25740
ttgtgtttgt atgtcctgtg gccaggaagt aatatgtgtg tgtgtatata tatatacatc 25800
tattgttgtg tttgtatgtc ctgtgtttgt gtttgttgta tgattgcatt gtatggtatg 25860
atacatctat tgttgtgttt gtatgtcctg tgtttgtgtt tgttgtatga ttgcattgta 25920
tggtatgtat ggttgttgtt gtatgttgta tgttactata ttgtgtttgt tgtatgattg 25980
cattgtatgg tatgtatggt tgttgttgta tgttgtatgt tactatattt gttggtatgt 26040
ggcattaaat aaaatatgtt gtatggttgt tgttgtatgt tgtatgttac tatatttgtt 26100
ggtatgtggc attaaataaa atatgttttg tggttctgtg tgttatgtgg ttgcgcccta 26160
ttgttggtat gtggcattaa ataaaatatg ttttgtggtt ctgtgtgtta tgtggttgcg 26220
ccctagtgag taacaactgt atttgtgttt gtggtatggg tttgtggttc tgtgtgttat 26280
gtggttgcgc cctagtgagt aacaactgta tttgtgtttg tggtatgggt gttgcttgtt 26340
gggctatata ttgtcctgta gtgagtaaca actgtatttg tgtttgtggt atgggtgttg 26400
cttgttgggc tatatattgt cctgtatttc aagttataaa actgcacacc ttacagcatc 26460
gggtgttgct tgttgggcta tatattgtcc tgtatttcaa gttataaaac tgcacacctt 26520
acagcatcca ttttatccta caatcctcca ttttgctgtg atttcaagtt ataaaactgc 26580
acaccttaca gcatccattt tatcctacaa tcctccattt tgctgtgcaa ccgatttcgg 26640
ttgcctttgg cttatgtctg tccattttat cctacaatcc tccattttgc tgtgcaaccg 26700
atttcggttg cctttggctt atgtctgtgg ttttctgcac aatacagtac gctggcacta 26760
gcaaccgatt tcggttgcct ttggcttatg tctgtggttt tctgcacaat acagtacgct 26820
ggcactattg caaactttaa tcttttgggc actgctccta gtggttttct gcacaataca 26880
gtacgctggc actattgcaa actttaatct tttgggcact gctcctacat attttgaaca 26940
attggcgcgc ctctttggcg attgcaaact ttaatctttt gggcactgct cctacatatt 27000
ttgaacaatt ggcgcgcctc tttggcgcat ataaggcgca cctggtatta gtcattttcc 27060
catattttga acaattggcg cgcctctttg gcgcatataa ggcgcacctg gtattagtca 27120
ttttcctgtc caggtgcgct acaacaattg cttgcataac cacctggtat tagtcatttt 27180
cctgtccagg tgcgctacaa caattgcttg cataactata tccactccct aagtaataaa 27240
actgctttta gctgtccagg tgcgctacaa caattgcttg cataactata tccactccct 27300
aagtaataaa actgctttta ggcacatatt ttagtttgtt tttacttaag cactatatcc 27360
actccctaag taataaaact gcttttaggc acatatttta gtttgttttt acttaagcta 27420
attgcatact tggcttgtac aactactttc attaggcaca tattttagtt tgtttttact 27480
taagctaatt gcatacttgg cttgtacaac tactttcatg tccaacattc tgtctaccct 27540
taacatgaac tgctaattgc atacttggct tgtacaacta ctttcatgtc caacattctg 27600
tctaccctta acatgaacta taatatgact aagctgtgca tacatagttt acatgtccaa 27660
cattctgtct acccttaaca tgaactataa tatgactaag ctgtgcatac atagtttcgt 27720
aaaccgaaat aggttgggca gcacatacta t 27751
<210> 3
<211> 29890
<212> DNA
<213> Artificial Sequence
<220>
<223> 探针型别HPV33
<400> 3
gtaaactata cgtacaagtt ttaaaaaagt agggtgtaac cgaaagcggt tcaaccgaaa 60
acggtgcata tataaagcaa acattttgca gtaaggtact ccgaaaacgg tgcatatata 120
aagcaaacat tttgcagtaa ggtactgcac gactatgttt caagacactg aggaaaaacc 180
acgaacattg catgatttgt cggtgcatat ataaagcaaa cattttgcag taaggtactg 240
cacgactatg tttcaagaca ctgaggaaaa accacgaaca ttgcatgatt tgtggcagta 300
aggtactgca cgactatgtt tcaagacact gaggaaaaac cacgaacatt gcatgatttg 360
tgccaagcat tggagacaac tatacacaac attgactgag gaaaaaccac gaacattgca 420
tgatttgtgc caagcattgg agacaactat acacaacatt gaactacagt gcgtggacgt 480
aaaaaaacct ttgctgccaa gcattggaga caactataca caacattgaa ctacagtgcg 540
tggacgtaaa aaaacctttg caacgatctg aggtatatga ttttgcattt gcaggaacta 600
cagtgcgtgg acgtaaaaaa acctttgcaa cgatctgagg tatatgattt tgcatttgca 660
gatttaacag ttgtatatag agagggaaat ccatagtgcg tggacgtaaa aaaacctttg 720
caacgatctg aggtatatga ttttgcattt gcagatttaa cagttgtata tagagaggga 780
aatccatttg gcaacgatct gaggtatatg attttgcatt tgcagattta acagttgtat 840
atagagaggg aaatccattt ggaatatgta aactgtgttt gcggttctta ctgaggtata 900
tgattttgca tttgcagatt taacagttgt atatagagag ggaaatccat ttggaatatg 960
taaactgtgt ttgcggttct tatctaaaat gatttaacag ttgtatatag agagggaaat 1020
ccatttggaa tatgtaaact gtgtttgcgg ttcttatcta aaattagtga atatagacat 1080
tataattatt tttggaatat gtaaactgtg tttgcggttc ttatctaaaa ttagtgaata 1140
tagacattat aattattctg tatatggaaa tacattagaa caaacagtta tctaaaatta 1200
gtgaatatag acattataat tattctgtat atggaaatac attagaacaa acagttaaaa 1260
aacctttaaa tgaaatatta attaggtgta tctgtatatg gaaatacatt agaacaaaca 1320
gttaaaaaac ctttaaatga aatattaatt aggtgtatta tatgtcaaag acctttgtgt 1380
cctcaagaaa tatatggaaa tacattagaa caaacagtta aaaaaccttt aaatgaaata 1440
ttaattaggt gtattatatg tcaaagacct ttgtgtcctc aagaaaaaaa aaacctttaa 1500
atgaaatatt aattaggtgt attatatgtc aaagaccttt gtgtcctcaa gaaaaaaaac 1560
gacatgtgga tttaaacaaa cgatttcatg tcaaagacct ttgtgtcctc aagaaaaaaa 1620
acgacatgtg gatttaaaca aacgatttca taatatttcg ggtcgttggg cagggcgctg 1680
tgcggcgcat aatatttcgg gtcgttgggc agggcgctgt gcggcgtgtt ggaggtcccg 1740
acgtagagaa actgcactgt gacgtgtaaa aacgccatga gaggacagcg gcgtgttgga 1800
ggtcccgacg tagagaaact gcactgtgac gtgtaaaaac gccatgagag gacacaagcc 1860
aacgttaaag gaatatgttt tagatttgca ctgtgacgtg taaaaacgcc atgagaggac 1920
acaagccaac gttaaaggaa tatgttttag atttatatcc tgaaccaact gacctatact 1980
gctatgatgt aaaaacgcca tgagaggaca caagccaacg ttaaaggaat atgttttaga 2040
tttatatcct gaaccaactg acctatactg ctatgagcaa ttacaagcca acgttaaagg 2100
aatatgtttt agatttatat cctgaaccaa ctgacctata ctgctatgag caattaagtg 2160
acagctcaga tgaggatgaa ggcatatcct gaaccaactg acctatactg ctatgagcaa 2220
ttaagtgaca gctcagatga ggatgaaggc ttggaccggc cagatggaca agcacaacca 2280
gcctgaacca actgacctat actgctatga gcaattaagt gacagctcag atgaggatga 2340
aggcttggac cggccagatg gacaagcaca accagccaca gcaattaagt gacagctcag 2400
atgaggatga aggcttggac cggccagatg gacaagcaca accagccaca gctgattact 2460
acattgtaac ctgttgtcac ggcttggacc ggccagatgg acaagcacaa ccagccacag 2520
ctgattacta cattgtaacc tgttgtcaca cttgtaacac cacagttcgt ttatgtgtca 2580
gccacagctg attactacat tgtaacctgt tgtcacactt gtaacaccac agttcgttta 2640
tgtgtcaaca gtacagcaag tgacctacga accatacagc acacttgtaa caccacagtt 2700
cgtttatgtg tcaacagtac agcaagtgac ctacgaacca tacagcaact acttatgggc 2760
acagtgaata ttgtgtgccc cagtacagca agtgacctac gaaccataca gcaactactt 2820
atgggcacag tgaatattgt gtgccctacc tgtgcacaac aataaacatc atctacaatg 2880
cctacctgtg cacaacaata aacatcatct acaatggccg atcctgaagg tacaaatggg 2940
gctgggatgg ggtgtactgg ttggtttgag gtagaagcag tggccgatcc tgaaggtaca 3000
aatggggctg ggatggggtg tactggttgg tttgaggtag aagcagtcat agagagaaga 3060
acaggagata atatttcaga gatcctgaag gtacaaatgg ggctgggatg gggtgtactg 3120
gttggtttga ggtagaagca gtcatagaga gaagaacagg agataatatt tcagaagatg 3180
ggggtgtact ggttggtttg aggtagaagc agtcatagag agaagaacag gagataatat 3240
ttcagaagat gaggatgaaa cagcagatga cagtggcacg gtcatagaga gaagaacagg 3300
agataatatt tcagaagatg aggatgaaac agcagatgac agtggcacgg atttactaga 3360
gtttatagat gattctatgg gatgaggatg aaacagcaga tgacagtggc acggatttac 3420
tagagtttat agatgattct atggaaaata gtatacaggc agacacagag gcagcccggg 3480
cggatttact agagtttata gatgattcta tggaaaatag tatacaggca gacacagagg 3540
cagcccgggc attgtttaat atacaggaag gggaggatga ttatagatga ttctatggaa 3600
aatagtatac aggcagacac agaggcagcc cgggcattgt ttaatataca ggaaggggag 3660
gatgatttaa cgtatgtgtg gaaaatagta tacaggcaga cacagaggca gcccgggcat 3720
tgtttaatat acaggaaggg gaggatgatt taacgtatgt gtgtgcacta aaacgaaaga 3780
caggcagaca cagaggcagc ccgggcattg tttaatatac aggaagggga ggatgattta 3840
acgtatgtgt gtgcactaaa acgaaagttt gccgccgggc attgtttaat atacaggaag 3900
gggaggatga tttaacgtat gtgtgtgcac taaaacgaaa gtttgccgca tgttcacaaa 3960
gtgctgcgga ggacgttgtt taatatacag gaaggggagg atgatttaac gtatgtgtgt 4020
gcactaaaac gaaagtttgc cgcatgttca caaagtgctg cggaggacat ttaacgtatg 4080
tgtgtgcact aaaacgaaag tttgccgcat gttcacaaag tgctgcggag gacgttgttg 4140
atcgtgctgc aaacccgtgt agaacgtctt gccgcatgtt cacaaagtgc tgcggaggac 4200
gttgttgatc gtgctgcaaa cccgtgtaga acgtctatta ataaaaataa agacgtaaca 4260
tacagaaacg ttgttgatcg tgctgcaaac ccgtgtagaa cgtctattaa taaaaataaa 4320
gacgtaacat acagaaaacg aaaaatagat gagctagaag acagcggact attaataaaa 4380
ataaagacgt aacatacaga aaacgaaaaa tagatgagct agaagacagc ggatatggca 4440
atactgaagt ggaaactcag cagatggtat aaaaataaag acgtaacata cagaaaacga 4500
aaaatagatg agctagaaga cagcggatat ggcaatactg aagtggaaac tcagcagatg 4560
gacgaaaaat agatgagcta gaagacagcg gatatggcaa tactgaagtg gaaactcagc 4620
agatggtaca acaggtagaa agtcaaaatg gcgacacaaa catggcaata ctgaagtgga 4680
aactcagcag atggtacaac aggtagaaag tcaaaatggc gacacaaact taaatgactt 4740
agaatctagt ggggtggggg aaatactgaa gtggaaactc agcagatggt acaacaggta 4800
gaaagtcaaa atggcgacac aaacttaaat gacttagaat ctagtggggt ggggggtaca 4860
acaggtagaa agtcaaaatg gcgacacaaa cttaaatgac ttagaatcta gtggggtggg 4920
ggatgattca gaagtaagct gtgagacaaa tgtagactta aatgacttag aatctagtgg 4980
ggtgggggat gattcagaag taagctgtga gacaaatgta gatagctgtg aaaatgttac 5040
gttgcaggaa attaggatag ctgtgaaaat gttacgttgc aggaaattag taatgttcta 5100
catagtagta atacaaaagc aaatatatta tataaattta aagaggccta tggaagtaat 5160
gttctacata gtagtaatac aaaagcaaat atattatata aatttaaaga ggcctatgga 5220
ataagtttta tggaattagt aagaccattt aaaagtgttc tacatagtag taatacaaaa 5280
gcaaatatat tatataaatt taaagaggcc tatggaataa gttttatgga attagtaaga 5340
ccatttaaaa gtgatatata ttatataaat ttaaagaggc ctatggaata agttttatgg 5400
aattagtaag accatttaaa agtgataaaa caagctgtac agattggtgt ataacattat 5460
ataaatttaa agaggcctat ggaataagtt ttatggaatt agtaagacca tttaaaagtg 5520
ataaaacaag ctgtacagat tggtgtataa caggtaagtt ttatggaatt agtaagacca 5580
tttaaaagtg ataaaacaag ctgtacagat tggtgtataa caggatatgg aattagtcca 5640
tcagtagcag aaaggtttta tggaattagt aagaccattt aaaagtgata aaacaagctg 5700
tacagattgg tgtataacag gatatggaat tagtccatca gtagcagaaa gtttgtaaga 5760
ccatttaaaa gtgataaaac aagctgtaca gattggtgta taacaggata tggaattagt 5820
ccatcagtag cagaaagttt aaaagtgata aaacaagctg tacagattgg tgtataacag 5880
gatatggaat tagtccatca gtagcagaaa gtttaaaagt attaattaaa cagcatagtt 5940
tgtacaggat atggaattag tccatcagta gcagaaagtt taaaagtatt aattaaacag 6000
catagtttgt atactcattt acaatgttta acttgcgata gagggtttaa aagtattaat 6060
taaacagcat agtttgtata ctcatttaca atgtttaact tgcgatagag gaataataat 6120
attattgtta attagattta ggtgtactca tttacaatgt ttaacttgcg atagaggaat 6180
aataatatta ttgttaatta gatttaggtg tagcaaaaac aggttaacag tagcaaaact 6240
aatgggaata ataatattat tgttaattag atttaggtgt agcaaaaaca ggttaacagt 6300
agcaaaacta atgagtaatt tattatcaat acctgaaaca tgtagtagca aaaacaggtt 6360
aacagtagca aaactaatga gtaatttatt atcaatacct gaaacatgta tggttataga 6420
gccaccaaaa ttacggagcc aaactgagta atttattatc aatacctgaa acatgtatgg 6480
ttatagagcc accaaaatta cggagccaaa catgtgcatt gtattggttt agaacagcaa 6540
tgtcgtaatt tattatcaat acctgaaaca tgtatggtta tagagccacc aaaattacgg 6600
agccaaacat gtgcattgta ttggtttaga acagcaatgt caaatggtta tagagccacc 6660
aaaattacgg agccaaacat gtgcattgta ttggtttaga acagcaatgt caaacattag 6720
tgatgtacaa ggtacaacac ctgatataga gccaccaaaa ttacggagcc aaacatgtgc 6780
attgtattgg tttagaacag caatgtcaaa cattagtgat gtacaaggta caacacctga 6840
atggcatgtg cattgtattg gtttagaaca gcaatgtcaa acattagtga tgtacaaggt 6900
acaacacctg aatggataga tagactaact gttttacaac atagtattgg tttagaacag 6960
caatgtcaaa cattagtgat gtacaaggta caacacctga atggatagat agactaactg 7020
ttttacaaca tagcttcaaa cattagtgat gtacaaggta caacacctga atggatagat 7080
agactaactg ttttacaaca tagctttaat gataatatat ttgatttaag tgaaattgga 7140
tagatagact aactgtttta caacatagct ttaatgataa tatatttgat ttaagtgaaa 7200
tggtacagtg ggcatatgat aacgagttaa cggacggctt taatgataat atatttgatt 7260
taagtgaaat ggtacagtgg gcatatgata acgagttaac ggacgatagt gacattgcat 7320
attattcgta acaactaatg ataatatatt tgatttaagt gaaatggtac agtgggcata 7380
tgataacgag ttaacggacg atagtgacat tgcatattat tcgtaacatg gtacagtggg 7440
catatgataa cgagttaacg gacgatagtg acattgcata ttattcgtaa caacttgcag 7500
attcaaatag tacgtatgct gcatttttga cgatagtgac attgcatatt attcgtaaca 7560
acttgcagat tcaaatagta cgtatgctgc atttttaaaa agtaactcac aagcaaaaat 7620
agtaaagggt gacattgcat attattcgta acaacttgca gattcaaata gtacgtatgc 7680
tgcattttta aaaagtaact cacaagcaaa aatagtaaag gactgtgggc acaacttgca 7740
gattcaaata gtacgtatgc tgcattttta aaaagtaact cacaagcaaa aatagtaaag 7800
gactgtggaa taatgtgtag acattattgc agattcaaat agtacgtatg ctgcattttt 7860
aaaaagtaac tcacaagcaa aaatagtaaa ggactgtgga ataatgtgta gacattataa 7920
aaaagcagta cgtatgctgc atttttaaaa agtaactcac aagcaaaaat agtaaaggac 7980
tgtggaataa tgtgtagaca ttataaaaaa gcagaaacgt atgctgcatt tttaaaaagt 8040
aactcacaag caaaaatagt aaaggactgt ggaataatgt gtagacatta taaaaaagca 8100
gaaaaacgta aaatgtctaa aaagtaactc acaagcaaaa atagtaaagg actgtggaat 8160
aatgtgtaga cattataaaa aagcagaaaa acgtaaaatg tcaataggac aatggatact 8220
gtggaataat gtgtagacat tataaaaaag cagaaaaacg taaaatgtca ataggacaat 8280
ggatacaaag tagatgtgaa aaaacaaatg atggaggatg tgtagacatt ataaaaaagc 8340
agaaaaacgt aaaatgtcaa taggacaatg gatacaaagt agatgtgaaa aaacaaatga 8400
tggaggaaag cagaaaaacg taaaatgtca ataggacaat ggatacaaag tagatgtgaa 8460
aaaacaaatg atggaggaaa ttggagacca atagtacagt tgttaagatt acaaagtaga 8520
tgtgaaaaaa caaatgatgg aggaaattgg agaccaatag tacagttgtt aagatatcaa 8580
aacattgaat ttacagcatt tttaggtgca agtagatgtg aaaaaacaaa tgatggagga 8640
aattggagac caatagtaca gttgttaaga tatcaaaaca ttgaatttac agcattttta 8700
ggtgcatttg gaaattggag accaatagta cagttgttaa gatatcaaaa cattgaattt 8760
acagcatttt taggtgcatt taaaaagttt ttaaaaggta taccaaaaat tggagaccaa 8820
tagtacagtt gttaagatat caaaacattg aatttacagc atttttaggt gcatttaaaa 8880
agtttttaaa aggtatacca aaaaaaagat caaaacattg aatttacagc atttttaggt 8940
gcatttaaaa agtttttaaa aggtatacca aaaaaaagct gtcgtataat ttgtggacca 9000
gcaaatacaa acattgaatt tacagcattt ttaggtgcat ttaaaaagtt tttaaaaggt 9060
ataccaaaaa aaagctgtcg tataatttgt ggaccagcaa atacagggca tttaaaaagt 9120
ttttaaaagg tataccaaaa aaaagctgtc gtataatttg tggaccagca aatacaggaa 9180
agtcatattt tggaatgagt ttaatacaaa agtttttaaa aggtatacca aaaaaaagct 9240
gtcgtataat ttgtggacca gcaaatacag gaaagtcata ttttggaatg agtttaataa 9300
agctgtcgta taatttgtgg accagcaaat acaggaaagt catattttgg aatgagttta 9360
atacagtttt taaaagggtg tgttatatca tgtgtaaaca ggaaagtcat attttggaat 9420
gagtttaata cagtttttaa aagggtgtgt tatatcatgt gtaaattcta aaagtcactt 9480
ttggttgcag ccattatcgg aaagtcatat tttggaatga gtttaataca gtttttaaaa 9540
gggtgtgtta tatcatgtgt aaattctaaa agtcactttt ggttgcagcc attatcaggt 9600
ttttaaaagg gtgtgttata tcatgtgtaa attctaaaag tcacttttgg ttgcagccat 9660
tatcagcgta aaaaatagga atgatagatg atgtaacgtt taaaagggtg tgttatatca 9720
tgtgtaaatt ctaaaagtca cttttggttg cagccattat cagcgtaaaa aataggaatg 9780
atagatgatg taacttctaa aagtcacttt tggttgcagc cattatcagc gtaaaaaata 9840
ggaatgatag atgatgtaac gccaataagt tggacatata tagatgatta catgctaaaa 9900
gtcacttttg gttgcagcca ttatcagcgt aaaaaatagg aatgatagat gatgtaacgc 9960
caataagttg gacatatata gatgattaca tgagcagcgt aaaaaatagg aatgatagat 10020
gatgtaacgc caataagttg gacatatata gatgattaca tgagaacgta gttagatgga 10080
aatgaaattt caattaggaa tgatagatga tgtaacgcca ataagttgga catatataga 10140
tgattacatg agaacgtagt tagatggaaa tgaaatttca atagatgcgc caataagttg 10200
gacatatata gatgattaca tgagaacgta gttagatgga aatgaaattt caatagatgt 10260
gaaacatagg gcattagtgc aattaaacaa taagttggac atatatagat gattacatga 10320
gaacgtagtt agatggaaat gaaatttcaa tagatgtgaa acatagggca ttagtgcaat 10380
taaaatgtga gaacgtagtt agatggaaat gaaatttcaa tagatgtgaa acatagggca 10440
ttagtgcaat taaaatgtcc accactgctt cttacctcaa atacaaatgc gttagatgga 10500
aatgaaattt caatagatgt gaaacatagg gcattagtgc aattaaaatg tccaccactg 10560
cttcttacct caaatacaac gtaaggctag atgtgaaaca tagggcatta gtgcaattaa 10620
aatgtccacc actgcttctt acctcaaata caacgtaagg cacagactct agatggccat 10680
atttacaaat gtccaccact gcttcttacc tcaaatacaa cgtaaggcac agactctaga 10740
tggccatatt tacatagtag attaacagta tttgaattta aaaatcccgt aaggcacaga 10800
ctctagatgg ccatatttac atagtagatt aacagtattt gaatttaaaa atccattccc 10860
atttgatgaa aatggtaacc cagtgtacat agtagattaa cagtatttga atttaaaaat 10920
ccattcccat ttgatgaaaa tggtaaccca gtgtcgtaaa taaatgatga aaattggaaa 10980
tccttttgta gattaacagt atttgaattt aaaaatccat tcccatttga tgaaaatggt 11040
aacccagtgt cgtaaataaa tgatgaaaat tggaaatcct ttttctcaaa tccattccca 11100
tttgatgaaa atggtaaccc agtgtcgtaa ataaatgatg aaaattggaa atcctttttc 11160
tcaaggacgt ggtgcaaatt agatccattc ccatttgatg aaaatggtaa cccagtgtcg 11220
taaataaatg atgaaaattg gaaatccttt ttctcaagga cgtggtgcaa attagattta 11280
atagttccca tttgatgaaa atggtaaccc agtgtcgtaa ataaatgatg aaaattggaa 11340
atcctttttc tcaaggacgt ggtgcaaatt agatttccat ttgatgaaaa tggtaaccca 11400
gtgtcgtaaa taaatgatga aaattggaaa tcctttttct caaggacgtg gtgcaaatta 11460
gatttaatag aggaagccat ttgatgaaaa tggtaaccca gtgtcgtaaa taaatgatga 11520
aaattggaaa tcctttttct caaggacgtg gtgcaaatta gatttaatag aggaagtgca 11580
ataaatgatg aaaattggaa atcctttttc tcaaggacgt ggtgcaaatt agatttaata 11640
gaggaagagg acaaggaaaa ccatggagga aatatctctc aaggacgtgg tgcaaattag 11700
atttaataga ggaagaggac aaggaaaacc atggaggaaa tatcagcacg tttaacgtaa 11760
gtgcaggaga aaatacagga cgtggtgcaa attagattta atagaggaag aggacaagga 11820
aaaccatgga ggaaatatca gcacgtttaa cgtaagtgca ggagaaaata ctagaggacg 11880
tggtgcaaat tagatttaat agaggaagag gacaaggaaa accatggagg aaatatcagc 11940
acgtttaacg taagtgcagg agaaaatact agatcggaag aggacaagga aaaccatgga 12000
ggaaatatca gcacgtttaa cgtaagtgca ggagaaaata ctagatcttt acgaagctga 12060
taaaactgat ttaccagagg acaaggaaaa ccatggagga aatatcagca cgtttaacgt 12120
aagtgcagga gaaaatacta gatctttacg aagctgataa aactgattta ccatctcagc 12180
acgtttaacg taagtgcagg agaaaatact agatctttac gaagctgata aaactgattt 12240
accatcacaa attgaacatt ggaaactgat acgcactaga tctttacgaa gctgataaaa 12300
ctgatttacc atcacaaatt gaacattgga aactgatacg catggagtgt gctttattgt 12360
atacagccaa acaaaccatc acaaattgaa cattggaaac tgatacgcat ggagtgtgct 12420
ttattgtata cagccaaaca aatgggattt tcacatttcg tacaccaggt ggtgccacaa 12480
attgaacatt ggaaactgat acgcatggag tgtgctttat tgtatacagc caaacaaatg 12540
ggattttcac atttcgtaca ccaggtggtg ccttcatgga gtgtgcttta ttgtatacag 12600
ccaaacaaat gggattttca catttcgtac accaggtggt gccttctttg ttagcatcaa 12660
agaccaaagc atttcatggg attttcacat ttcgtacacc aggtggtgcc ttctttgtta 12720
gcatcaaaga ccaaagcatt tcaagtaatt gaactacaaa tggcattaga gacattcaca 12780
tttcgtacac caggtggtgc cttctttgtt agcatcaaag accaaagcat ttcaagtaat 12840
tgaactacaa atggcattag agacattagc cttctttgtt agcatcaaag accaaagcat 12900
ttcaagtaat tgaactacaa atggcattag agacattaag taaatcacag tatagtacaa 12960
gccaatggca agtaattgaa ctacaaatgg cattagagac attaagtaaa tcacagtata 13020
gtacaagcca atggacattg caacaaacaa gcttagaggt gtggcttttt aagtaaatca 13080
cagtatagta caagccaatg gacattgcaa caaacaagct tagaggtgtg gctttgtgaa 13140
ccaccaaaat gttttaaaaa acaaggaggg acattgcaac aaacaagctt agaggtgtgg 13200
ctttgtgaac caccaaaatg ttttaaaaaa caaggagaaa cagtaactgt gcaatatgac 13260
aatgacaagc aacaaacaag cttagaggtg tggctttgtg aaccaccaaa atgttttaaa 13320
aaacaaggag aaacagtaac tgtgcaatat gacaatgatg tgaaccacca aaatgtttta 13380
aaaaacaagg agaaacagta actgtgcaat atgacaatga caaaaaaaat acaatggatt 13440
atacaaactg gggtgaaaga aacagtaact gtgcaatatg acaatgacaa aaaaaataca 13500
atggattata caaactgggg tgaaatatat attatagagg aagatacatg tactatggaa 13560
aaatacaatg gattatacaa actggggtga aatatatatt atagaggaag atacatgtac 13620
tatggttaca gggaaagtag attatatagg tatgtattat atatattata gaggaagata 13680
catgtactat ggttacaggg aaagtagatt atataggtat gtattatata cataactgtg 13740
aaaaggtata ttttaaatgg ttacagggaa agtagattat ataggtatgt attatataca 13800
taactgtgaa aaggtatatt ttaaatattt taaagaggcg tatgcaaagt attctaaata 13860
tatacataac tgtgaaaagg tatattttaa atattttaaa gaggcgtatg caaagtattc 13920
taaaacacaa atgtgggaag tacatgtggg tggtcaggta ttttaaagag gcgtatgcaa 13980
agtattctaa aacacaaatg tgggaagtac atgtgggtgg tcaggtaatt gtttgtccta 14040
cgtctatatc tagcaaccca caaatgtggg aagtacatgt gggtggtcag gtaattgttt 14100
gtcctacgtc tatatctagc aaccaaatat ccactactga aactgctgac atacagacgt 14160
aattgtttgt cctacgtcta tatctagcaa ccaaatatcc actactgaaa ctgctgacat 14220
acagacagac aacgataacc gaccaccaca agcagcggca aatatccact actgaaactg 14280
ctgacataca gacagacaac gataaccgac caccacaagc agcggccaaa cgacgacgac 14340
ctgcagacac cacagacaac agacaacgat aaccgaccac cacaagcagc ggccaaacga 14400
cgacgacctg cagacaccac agacaccgcc cagcccctta caaagctgtt ctgtgcaggc 14460
caaacgacga cgacctgcag acaccacaga caccgcccag ccccttacaa agctgttctg 14520
tgcagacccc gccttggaca atagaacagc acgtactgac cgcccagccc cttacaaagc 14580
tgttctgtgc agaccccgcc ttggacaata gaacagcacg tactgcaact aactgcacaa 14640
acaagcagcg gactgtgtga ccccgccttg gacaatagaa cagcacgtac tgcaactaac 14700
tgcacaaaca agcagcggac tgtgtgtagt tctaacgttg cacctatagt gcatttaagc 14760
aactaactgc acaaacaagc agcggactgt gtgtagttct aacgttgcac ctatagtgca 14820
tttaaaaggt gaatcaaata gtttaaaatg tttaagatgt gtagttctaa cgttgcacct 14880
atagtgcatt taaaaggtga atcaaatagt ttaaaatgtt taagatacag attaaaacct 14940
tataaagagt tgtatagtgt gcatttaaaa ggtgaatcaa atagtttaaa atgtttaaga 15000
tacagattaa aaccttataa agagttgtat agttctatgt catccacctg gcattggtaa 15060
aaggtgaatc aaatagttta aaatgtttaa gatacagatt aaaaccttat aaagagttgt 15120
atagttctat gtcatccacc tggcattgga ccagtaaagg tgaatcaaat agtttaaaat 15180
gtttaagata cagattaaaa ccttataaag agttgtatag ttctatgtca tccacctggc 15240
attggaccag tgacaacaga ttaaaacctt ataaagagtt gtatagttct atgtcatcca 15300
cctggcattg gaccagtgac aacaaaaata gtaaaaatgg aattgtaact gtaactctat 15360
gtcatccacc tggcattgga ccagtgacaa caaaaatagt aaaaatggaa ttgtaactgt 15420
aacatttgta actgaacagc aacaacaaat gttttcaaca aaaatagtaa aaatggaatt 15480
gtaactgtaa catttgtaac tgaacagcaa caacaaatgt ttttaggtac cgtaaaaata 15540
ccacctactg tgcaaacatt tgtaactgaa cagcaacaac aaatgttttt aggtaccgta 15600
aaaataccac ctactgtgca aataagtact ggatttatga cattataagt gtacattagg 15660
taccgtaaaa ataccaccta ctgtgcaaat aagtactgga tttatgacat tataagtgta 15720
catcacaagc caatatgtgc tgctaattgt atataataag tactggattt atgacattat 15780
aagtgtacat cacaagccaa tatgtgctgc taattgtata taaccatgat atttgttttt 15840
gtattatgtt ttatacatca caagccaata tgtgctgcta attgtatata accatgatat 15900
ttgtttttgt attatgtttt atattgtttt tcgtattatc cttattatta cgtccaccat 15960
gatatttgtt tttgtattat gttttatatt gtttttcgta ttatccttat tattacgtcc 16020
tttaatactt tccatttcta cctcgtattg gttgctgttt ttcgtattat ccttattatt 16080
acgtccttta atactttcca tttctacctc gtattggttg ctggtgttgg tattgctgct 16140
ttgggtgttt gtgggtttaa tactttccat ttctacctcg tattggttgc tggtgttggt 16200
attgctgctt tgggtgtttg tgggatctcc tttaaaaatt tttttttgct atttggctgg 16260
tgttggtatt gctgctttgg gtgtttgtgg gatctccttt aaaaattttt ttttgctatt 16320
tgttgttttt atatttacca atgatgtgta ttaatgctgg tgttggtatt gctgctttgg 16380
gtgtttgtgg gatctccttt aaaaattttt ttttgctatt tgttgttttt atatttacca 16440
atgatgtgta ttaatgggat ctcctttaaa aatttttttt tgctatttgt tgtttttata 16500
tttaccaatg atgtgtatta attttccgta acagcatatg acacaacaag agtaagttgt 16560
ttttatattt accaatgatg tgtattaatt ttccgtaaca gcatatgaca caacaagagt 16620
aatgtatata catgtatata ttgtttgtat atatgttttc cgtaacagca tatgacacaa 16680
caagagtaat gtatatacat gtatatattg tttgtatata tgtgcacatg gtggtgtttt 16740
aacattgttg ttgttatgta tatacatgta tatattgttt gtatatatgt gcacatggtg 16800
gtgttttaac attgttgttg ttattttagt tttttttttt ttgtattact aataagtgca 16860
catggtggtg ttttaacatt gttgttgtta ttttagtttt tttttttttg tattactaat 16920
aaataccttt atattttagc agtgtattat tatgaatttt agtttttttt tttttgtatt 16980
actaataaat acctttatat tttagcagtg tattattatg agacacaaac gatctacaag 17040
gcgcaagcgt gcatcatacc tttatatttt agcagtgtat tattatgaga cacaaacgat 17100
ctacaaggcg caagcgtgca tctgcaacac aactatacca aaccgtaaag gccactttta 17160
gcagtgtatt attatgagac acaaacgatc tacaaggcgc aagcgtgcat ctgcaacaca 17220
actataccaa accgtaaagg ccacaggcac cagacacaaa cgatctacaa ggcgcaagcg 17280
tgcatctgca acacaactat accaaaccgt aaaggccaca ggcacctgcc cacccgatgt 17340
tattcctaaa gtctgcaaca caactatacc aaaccgtaaa ggccacaggc acctgcccac 17400
ccgatgttat tcctaaagtg gaaggaagta ccatagcaga tcaaattctt acaaaccgta 17460
aaggccacag gcacctgccc acccgatgtt attcctaaag tggaaggaag taccatagca 17520
gatcaaattc ttaaatatgg cagtttaggg gccacaggca cctgcccacc cgatgttatt 17580
cctaaagtgg aaggaagtac catagcagat caaattctta aatatggcag tttaggggtt 17640
ttttttggtg ggtggaagga agtaccatag cagatcaaat tcttaaatat ggcagtttag 17700
gggttttttt tggtggttta ggtattggca caggctctgg ttcaggtgga aaatatggca 17760
gtttaggggt tttttttggt ggtttaggta ttggcacagg ctctggttca ggtggaagga 17820
ctggctatgt acctattggt actgacccac ctttaggtat tggcacaggc tctggttcag 17880
gtggaaggac tggctatgta cctattggta ctgacccacc tacagctgca atccccttgc 17940
agcctatacg taaggactgg ctatgtacct attggtactg acccacctac agctgcaatc 18000
cccttgcagc ctatacgtcc tccggttact gtagacactg ttggaccttt acctacagct 18060
gcaatcccct tgcagcctat acgtcctccg gttactgtag acactgttgg acctttagac 18120
tcgtctatag tgtcattaat agaagaaaca acctccggtt actgtagaca ctgttggacc 18180
tttagactcg tctatagtgt cattaataga agaaacaagt tttatagagg caggtgcacc 18240
agccccatct agactcgtct atagtgtcat taatagaaga aacaagtttt atagaggcag 18300
gtgcaccagc cccatctatt cctacaccat caggttttga tgttactaca tgttttatag 18360
aggcaggtgc accagcccca tctattccta caccatcagg ttttgatgtt actacatctg 18420
cagatactac acctgcaatt attaatgttt cttcctacac catcaggttt tgatgttact 18480
acatctgcag atactacacc tgcaattatt aatgtttcat ctgttgggga gtcatctatt 18540
caaactattt cctgcagata ctacacctgc aattattaat gtttcatctg ttggggagtc 18600
atctattcaa actatttcta cacatttaaa tcccacattt actgaaccat cctacacctg 18660
caattattaa tgtttcatct gttggggagt catctattca aactatttct acacatttaa 18720
atcccacatt tactgaacca tctgtactca tctgttgggg agtcatctat tcaaactatt 18780
tctacacatt taaatcccac atttactgaa ccatctgtac tacaccctcc agcgcctgca 18840
gaagcctcct acacatttaa atcccacatt tactgaacca tctgtactac accctccagc 18900
gcctgcagaa gcctctggac attttatatt ttcttcccct actgttagca tttaaatccc 18960
acatttactg aaccatctgt actacaccct ccagcgcctg cagaagcctc tggacatttt 19020
atattttctt cccctactgt tagcacacct gtactacacc ctccagcgcc tgcagaagcc 19080
tctggacatt ttatattttc ttcccctact gttagcacac aaagttatga aaacatacca 19140
atggatacag cacacaaagt tatgaaaaca taccaatgga tacctttgtt gtttccacag 19200
acagtagtaa tgtaacatca agcacgccca ttccagggtc tcgccctgag ttatgaaaac 19260
ataccaatgg atacctttgt tgtttccaca gacagtagta atgtaacatc aagcacgccc 19320
attccagggt ctcgccctgt ggcacgccac ctttgttgtt tccacagaca gtagtaatgt 19380
aacatcaagc acgcccattc cagggtctcg ccctgtggca cgccttggtt tatatagtcg 19440
caatacccca tcaagcacgc ccattccagg gtctcgccct gtggcacgcc ttggtttata 19500
tagtcgcaat acccaacagg ttaaggttgt tgaccctgct tttttaacgt ggcacgcctt 19560
ggtttatata gtcgcaatac ccaacaggtt aaggttgttg accctgcttt tttaacatcg 19620
cctcataaac ttataacata tgataatcca cgccttggtt tatatagtcg caatacccaa 19680
caggttaagg ttgttgaccc tgctttttta acatcgcctc ataaacttat aacatatgat 19740
aatcctgcca cgccttggtt tatatagtcg caatacccaa caggttaagg ttgttgaccc 19800
tgctttttta acatcgcctc ataaacttat aacatatgat aatcctgccg ccttggttta 19860
tatagtcgca atacccaaca ggttaaggtt gttgaccctg cttttttaac atcgcctcat 19920
aaacttataa catatgataa tcctgcatcc caacaggtta aggttgttga ccctgctttt 19980
ttaacatcgc ctcataaact tataacatat gataatcctg catttgaaag ctttgaccct 20040
gaagacacgt taaggttgtt gaccctgctt ttttaacatc gcctcataaa cttataacat 20100
atgataatcc tgcatttgaa agctttgacc ctgaagacac attcatcgcc tcataaactt 20160
ataacatatg ataatcctgc atttgaaagc tttgaccctg aagacacatt acaatttcaa 20220
catagtgata tatcacctgc tccctcataa acttataaca tatgataatc ctgcatttga 20280
aagctttgac cctgaagaca cattacaatt tcaacatagt gatatatcac ctgctcctga 20340
tcccctgcat ttgaaagctt tgaccctgaa gacacattac aatttcaaca tagtgatata 20400
tcacctgctc ctgatcctga ctttctagat attattgcat tacttgaaag ctttgaccct 20460
gaagacacat tacaatttca acatagtgat atatcacctg ctcctgatcc tgactttcta 20520
gatattattg cattacatta caatttcaac atagtgatat atcacctgct cctgatcctg 20580
actttctaga tattattgca ttacataggc ctgctattac atctcgtaga catactgcct 20640
gatcctgact ttctagatat tattgcatta cataggcctg ctattacatc tcgtagacat 20700
actgtgcgtt ttagtagagt aggtcaaaaa gccacaccat aggcctgcta ttacatctcg 20760
tagacatact gtgcgtttta gtagagtagg tcaaaaagcc acacttaaaa ctcgcagtgg 20820
taaacaaatt ggagctagtg cgttttagta gagtaggtca aaaagccaca cttaaaactc 20880
gcagtggtaa acaaattgga gctagaatac attattatca ggatttaagt cctattgctt 20940
aaaactcgca gtggtaaaca aattggagct agaatacatt attatcagga tttaagtcct 21000
attgtgcctt tagaccacac cgtgccaaat gaacaatgaa tacattatta tcaggattta 21060
agtcctattg tgcctttaga ccacaccgtg ccaaatgaac aatatgaatt acagccttta 21120
catgatactt ctacatcgtg cctttagacc acaccgtgcc aaatgaacaa tatgaattac 21180
agcctttaca tgatacttct acatcgtctt atagtattaa tgatggtttg tatgatgatg 21240
aattacagcc tttacatgat acttctacat cgtcttatag tattaatgat ggtttgtatg 21300
atgtttcgta tgacgatgtg gataatgtac acacccccgt cttatagtat taatgatggt 21360
ttgtatgatg tttcgtatga cgatgtggat aatgtacaca ccccacgtaa acactcatac 21420
agtacgtttg caacaacgtt tcgtatgacg atgtggataa tgtacacacc ccacgtaaac 21480
actcatacag tacgtttgca acaacacgta ccagcaatgt gtctatacct ttaaatacca 21540
cgtaaacact catacagtac gtttgcaaca acacgtacca gcaatgtgtc tataccttta 21600
aatacaggat ttgatactcc tgttatgtct ggccctgcac gtaccagcaa tgtgtctata 21660
cctttaaata caggatttga tactcctgtt atgtctggcc ctgatatacc ttccccttta 21720
tttcccacat ctagcccaca ggatttgata ctcctgttat gtctggccct gatatacctt 21780
cccctttatt tcccacatct agcccatttg ttcctatttc gccttttttt ccttttggat 21840
ataccttccc ctttatttcc cacatctagc ccatttgttc ctatttcgcc tttttttcct 21900
tttgacacca ttgttgtaga cggtgctgac tttgtttcca tttgttccta tttcgccttt 21960
ttttcctttt gacaccattg ttgtagacgg tgctgacttt gttttacatc ctagttattt 22020
tattttacgt cgcaggcgac accattgttg tagacggtgc tgactttgtt ttacatccta 22080
gttattttat tttacgtcgc aggcgtaaac gttttccata tttttttaca gatgtccgct 22140
gactttgttt tacatcctag ttattttatt ttacgtcgca ggcgtaaacg ttttccatat 22200
ttttttacag atgtccgtgt ggcggcctag tgaggccaca tcctagttat tttattttac 22260
gtcgcaggcg taaacgtttt ccatattttt ttacagatgt ccgtgtggcg gcctagtgag 22320
gccacagtgt acctgcctcg caggcgtaaa cgttttccat atttttttac agatgtccgt 22380
gtggcggcct agtgaggcca cagtgtacct gcctcctgta cctgtatcta aagttgtaga 22440
tgtccgtgtg gcggcctagt gaggccacag tgtacctgcc tcctgtacct gtatctaaag 22500
ttgtcagcac tgatgaatat gtgtctcgca caagcatccg tgtggcggcc tagtgaggcc 22560
acagtgtacc tgcctcctgt acctgtatct aaagttgtca gcactgatga atatgtgtct 22620
cgcacaagca tttattatgt ggcggcctag tgaggccaca gtgtacctgc ctcctgtacc 22680
tgtatctaaa gttgtcagca ctgatgaata tgtgtctcgc acaagcatgt gtacctgcct 22740
cctgtacctg tatctaaagt tgtcagcact gatgaatatg tgtctcgcac aagcatttat 22800
tattcgtatg gtagttccag acttgcctcc tgtacctgta tctaaagttg tcagcactga 22860
tgaatatgtg tctcgcacaa gcatttatta ttcgtatggt agttccagac ttcttgctgt 22920
tggcgatgaa tatgtgtctc gcacaagcat ttattattcg tatggtagtt ccagacttct 22980
tgctgttggc catccatatt tttctattaa aaatcctact aacgttcgta tggtagttcc 23040
agacttcttg ctgttggcca tccatatttt tctattaaaa atcctactaa cgctaaaaaa 23100
ttattggtac ccaaagtatc aggcgctgtt ggccatccat atttttctat taaaaatcct 23160
actaacgcta aaaaattatt ggtacccaaa gtatcaggct tgcaatatag ggtttttagg 23220
gtccgccatc catatttttc tattaaaaat cctactaacg ctaaaaaatt attggtaccc 23280
aaagtatcag gcttgcaata tagggttttt agggtccgtt tacccgctaa aaaattattg 23340
gtacccaaag tatcaggctt gcaatatagg gtttttaggg tccgtttacc agatcctaat 23400
aaatttggat ttcctgacac ctccgtatca ggcttgcaat atagggtttt tagggtccgt 23460
ttaccagatc ctaataaatt tggatttcct gacacctcct tttataaccc tgatagtatc 23520
aggcttgcaa tatagggttt ttagggtccg tttaccagat cctaataaat ttggatttcc 23580
tgacacctcc ttttataacc ctgatacaca acgcttgcaa tatagggttt ttagggtccg 23640
tttaccagat cctaataaat ttggatttcc tgacacctcc ttttataacc ctgatacaca 23700
acgattagta tggcgtttac cagatcctaa taaatttgga tttcctgaca cctcctttta 23760
taaccctgat acacaacgat tagtatgggc atgtgtaggc cttgaaatag gtgatcctaa 23820
taaatttgga tttcctgaca cctcctttta taaccctgat acacaacgat tagtatgggc 23880
atgtgtaggc cttgaaatag gtagagggca gcccttttat aaccctgata cacaacgatt 23940
agtatgggca tgtgtaggcc ttgaaatagg tagagggcag ccattaggcg ttggcataag 24000
tggtcatcct ttttttataa ccctgataca caacgattag tatgggcatg tgtaggcctt 24060
gaaataggta gagggcagcc attaggcgtt ggcataagtg gtctgggcat gtgtaggcct 24120
tgaaataggt agagggcagc cattaggcgt tggcataagt ggtcatcctt tattaaacaa 24180
atttgatgac actgaaaccg gcatgtgtag gccttgaaat aggtagaggg cagccattag 24240
gcgttggcat aagtggtcat cctttattaa acaaatttga tgacactgaa accggtaacg 24300
ccattaggcg ttggcataag tggtcatcct ttattaaaca aatttgatga cactgaaacc 24360
ggtaacaagt atcctggaca accgggtgct gataatagga ttaaacaaat ttgatgacac 24420
tgaaaccggt aacaagtatc ctggacaacc gggtgctgat aatagggaat gtttatccat 24480
ggattataaa caaacacagt tgatgacact gaaaccggta acaagtatcc tggacaaccg 24540
ggtgctgata atagggaatg tttatccatg gattataaac aaacacagtt atgtttactc 24600
aagtatcctg gacaaccggg tgctgataat agggaatgtt tatccatgga ttataaacaa 24660
acacagttat gtttacttgg atgtaagcct ccaacagggg ggaatgttta tccatggatt 24720
ataaacaaac acagttatgt ttacttggat gtaagcctcc aacaggggaa cattggggta 24780
aaggtgttgc ttgtactaag ttatgtttac ttggatgtaa gcctccaaca ggggaacatt 24840
ggggtaaagg tgttgcttgt actacgtaag cacctgccaa tgattgtcca cctttagaag 24900
gaacattggg gtaaaggtgt tgcttgtact acgtaagcac ctgccaatga ttgtccacct 24960
ttagaactta taaatactat tattgaggat ggtgatatgt gcagcacctg ccaatgattg 25020
tccaccttta gaacttataa atactattat tgaggatggt gatatggtgg acacaggatt 25080
tggttgcatg gattttaaac ttataaatac tattattgag gatggtgata tggtggacac 25140
aggatttggt tgcatggatt ttaaaacatt gcaggctaat aaaagtgatg ttcctattgg 25200
gtggacacag gatttggttg catggatttt aaaacattgc aggctaataa aagtgatgtt 25260
cctattgata tttgtggcag taccgtaaaa tatccagatc aggatttggt tgcatggatt 25320
ttaaaacatt gcaggctaat aaaagtgatg ttcctattga tatttgtggc agtaccgtaa 25380
aatatccaga ttatttaaca ttgcaggcta ataaaagtga tgttcctatt gatatttgtg 25440
gcagtaccgt aaaatatcca gattatttaa aaatgactag tgagccttat ggtgattgat 25500
atttgtggca gtaccgtaaa atatccagat tatttaaaaa tgactagtga gccttatggt 25560
gatagtttat ttttctttct tcgacgtgaa caaatgttat ttaaaaatga ctagtgagcc 25620
ttatggtgat agtttatttt tctttcttcg acgtgaacaa atgtttgtaa gacacttttt 25680
taatagggct ggtacatagt ttatttttct ttcttcgacg tgaacaaatg tttgtaagac 25740
acttttttaa tagggctggt acattaggag aggctgttcc cgatgacctg tacattgttt 25800
gtaagacact tttttaatag ggctggtaca ttaggagagg ctgttcccga tgacctgtac 25860
attaaaggtt caggaactac tgcctctatt caaagcttag gagaggctgt tcccgatgac 25920
ctgtacatta aaggttcagg aactactgcc tctattcaaa gcagtgcttt ttttcccact 25980
cctagtggat caatggtaaa ggttcaggaa ctactgcctc tattcaaagc agtgcttttt 26040
ttcccactcc tagtggatca atggttactt ccgaatctca gttatttaat aagccagcag 26100
tgcttttttt cccactccta gtggatcaat ggttacttcc gaatctcagt tatttaataa 26160
gccatattgg ctacaacgtg cacaaggtca taataaccta gtggatcaat ggttacttcc 26220
gaatctcagt tatttaataa gccatattgg ctacaacgtg cacaaggtca taataatggt 26280
atttgttggg ggttacttcc gaatctcagt tatttaataa gccatattgg ctacaacgtg 26340
cacaaggtca taataatggt atttgttggg gcaatcaggt atttgttact catattggct 26400
acaacgtgca caaggtcata ataatggtat ttgttggggc aatcaggtat ttgttactgt 26460
ggtagatacc actcgcagta ctaatatgac tggtatttgt tggggcaatc aggtatttgt 26520
tactgtggta gataccactc gcagtactaa tatgactttc gtaacacaag taactagtga 26580
cagtacatat gtggtagata ccactcgcag tactaatatg actttcgtaa cacaagtaac 26640
tagtgacagt acatataaaa atgaaaattt taaagaatat ataagacatg ttcgtaacac 26700
aagtaactag tgacagtaca tataaaaatg aaaattttaa agaatatata agacatgttg 26760
aagaatatga tctacagttt gtttttcaac taaaaatgaa aattttaaag aatatataag 26820
acatgttgaa gaatatgatc tacagtttgt ttttcaactc gtaaaagtta ccttaactgc 26880
agaagttatg gttgaagaat atgatctaca gtttgttttt caactcgtaa aagttacctt 26940
aactgcagaa gttatgacat atattccgta tatgaatcca gatattttag actcgtaaaa 27000
gttaccttaa ctgcagaagt tatgacatat attccgtata tgaatccaga tattttagaa 27060
gattggcaat ttggtttaac acctcctcca gacatatatt ccgtatatga atccagatat 27120
tttagaagat tggcaatttg gtttaacacc tcctccatct gctagtttac aggataccta 27180
taggtttgtt gaagattggc aatttggttt aacacctcct ccatctgcta gtttacagga 27240
tacctatagg tttgttacct ctcaggctat tacgtgtcaa aaaacagtac tacctctcag 27300
gctattacgt gtcaaaaaac agtacctcca aaggaaaagg aagacccctt aggtaaatat 27360
acattttggg aagtggattt aaaggaaaaa cctccaaagg aaaaggaaga ccccttaggt 27420
aaatatacat tttgggaagt ggatttaaag gaaaaatttt cagcagattt agatcagttt 27480
cctttgggac atatacattt tgggaagtgg atttaaagga aaaattttca gcagatttag 27540
atcagtttcc tttgggacgc aagtttttat tacaggcagg tcttaaagca attttcagca 27600
gatttagatc agtttccttt gggacgcaag tttttattac aggcaggtct taaagcaaaa 27660
cctaaactta aacgtgcagc ccccacatcc ggacgcaagt ttttattaca ggcaggtctt 27720
aaagcaaaac ctaaacttaa acgtgcagcc cccacatcca cccgcacatc gtctgcaaaa 27780
cgcaaaaagg gcaaaaccta aacttaaacg tgcagccccc acatccaccc gcacatcgtc 27840
tgcaaaacgc aaaaaggtta aaaaataaca ctttgtgtaa ttgtgttatg ccacccgcac 27900
atcgtctgca aaacgcaaaa aggttaaaaa ataacacttt gtgtaattgt gttatgttgt 27960
tgttttgttc tgtctatgta ctttgtgttg aaaaaataac actttgtgta attgtgttat 28020
gttgttgttt tgttctgtct atgtactttg tgttgttgtg ttgtgttgtt gtttgttttt 28080
tgtgtatgtg tgttgttttg ttctgtctat gtactttgtg ttgttgtgtt gtgttgttgt 28140
ttgttttttg tgtatgtgtt acaatgtatg ttatgttgta tgttactgtg gttgtgttgt 28200
gttgttgttt gttttttgtg tatgtgttac aatgtatgtt atgttgtatg ttactgtgtt 28260
tgttttatgt gtacttgttt gtgtgcatgt gtgttacaat gtatgttatg ttgtatgtta 28320
ctgtgtttgt tttatgtgta cttgtttgtg tgcatgttct atgtacttgt cagtttcctg 28380
tttgtgtata gtttgtttta tgtgtacttg tttgtgtgca tgttctatgt acttgtcagt 28440
ttcctgtttg tgtatatgtt aataaaacat tgtgtgtatt tgttaaacta tctatgtact 28500
tgtcagtttc ctgtttgtgt atatgttaat aaaacattgt gtgtatttgt taaactattt 28560
gtatgtatgt tatgtatatg ggtgtaccta tgttaataaa acattgtgtg tatttgttaa 28620
actatttgta tgtatgttat gtatatgggt gtacctatat gagtaaggag ttgtattgct 28680
tgccctaccc atttgtatgt atgttatgta tatgggtgta cctatatgag taaggagttg 28740
tattgcttgc cctaccctgc attgcaatgt acctaccttt atttccctat atgagtaagg 28800
agttgtattg cttgccctac cctgcattgc aatgtaccta cctttatttc cctatatttg 28860
tagtacctac atgtttagta ttgctttacc tatttgtagt acctacatgt ttagtattgc 28920
tttacctttt gacatactag tgtccatatt gtacaatttc ctccattttg tcgtactaac 28980
cgttttcggt accttttgac atactagtgt ccatattgta caatttcctc cattttgtcg 29040
tactaaccgt tttcggttac ttggcataca taccctatga cattggcaga ttcctccatt 29100
ttgtcgtact aaccgttttc ggttacttgg catacatacc ctatgacatt ggcagaacag 29160
ttaatccttt tctttcctgc actgtgtttg acagttaatc cttttctttc ctgcactgtg 29220
tttgtctgta cttgctgcat tggcatacat accctatgac attggcagaa cagttaatcc 29280
ttttctttcc cctgcactgt gtttgtctgt acttgctgca ttgactcata tataccgtaa 29340
gtgcaattgc aaaatactta attgtactaa tagtttacac cgtattttag actcatatat 29400
accgtaagtg caattgcaaa atacttaatt gtactaatag tttacaccgt attttaggca 29460
catattttta ctttactttc aaaccttaag cttaattgta ctaatagttt acaccgtatt 29520
ttaggcacat atttttactt tactttcaaa ccttaagtgc agttttggct tacacaattg 29580
ctttgtcgta ggcacatatt tttactttac tttcaaacct taagtgcagt tttggcttac 29640
acaattgctt tgtcgtacaa actcgtactt gtaaaagtga gtcactacct gtgcagtttt 29700
ggcttacaca attgctttgt cgtacaaact cgtacttgta aaagtgagtc actacctgtt 29760
tattaccagg tgtggactaa ccgttttagg gccaaactcg tacttgtaaa agtgagtcac 29820
tacctgttta ttaccaggtg tggactaacc gttttaggtc atattggtca tttataatct 29880
tttatataat 29890
<210> 4
<211> 24620
<212> DNA
<213> Artificial Sequence
<220>
<223> 探针型别HPV52
<400> 4
aaattataat cttatactag taaaaaatag ggtgtaaccg aaaacggtca gaccgaaacc 60
ggtgtatata tatagaacac agtgtagcta acgcacggcc cgaaaccggt gtatatatat 120
agaacacagt gtagctaacg cacggccatg tttgaggatc cagcaacacg accccggacc 180
ctgcacgaat tgtgtgaggt gctaacgcac ggccatgttt gaggatccag caacacgacc 240
ccggaccctg cacgaattgt gtgaggtgct ggaagaatcg gtgcatgaaa taaggctgca 300
acacgacccc ggaccctgca cgaattgtgt gaggtgctgg aagaatcggt gcatgaaata 360
aggctgcagt gtgtgcagtg caaaaaagag ctacaacgaa tgctggaaga atcggtgcat 420
gaaataaggc tgcagtgtgt gcagtgcaaa aaagagctac aacgaagaga ggtatacaag 480
tttctattta cagatttacg gtgtgtgcag tgcaaaaaag agctacaacg aagagaggta 540
tacaagtttc tatttacaga tttacgaata gtatatagag acaataatcc atatggcgtg 600
gagaggtata caagtttcta tttacagatt tacgaatagt atatagagac aataatccat 660
atggcgtgtg tattatgtgc ctacgctttt tatctaagat cgaatagtat atagagacaa 720
taatccatat ggcgtgtgta ttatgtgcct acgcttttta tctaagataa gtgaatatag 780
gcattatcaa tattcactgt tgtgtattat gtgcctacgc tttttatcta agataagtga 840
atataggcat tatcaatatt cactgtatgg gaaaacatta gaagagaggg taaaaaaacc 900
taagtgaata taggcattat caatattcac tgtatgggaa aacattagaa gagagggtaa 960
aaaaaccatt aagtgaaata actattagat gtataatttg atgggaaaac attagaagag 1020
agggtaaaaa aaccattaag tgaaataact attagatgta taatttgtca aacgccatta 1080
tgtcctgaag aaaaagaaag ccattaagtg aaataactat tagatgtata atttgtcaaa 1140
cgccattatg tcctgaagaa aaagaaagac atgttacgta aaacaagcga tttcataata 1200
gtcaaacgcc attatgtcct gaagaaaaag aaagacatgt tacgtaaaac aagcgatttc 1260
ataatattat gggtcgttgg acagggcgct gttcagagtg gacatgttac gtaaaacaag 1320
cgatttcata atattatggg tcgttggaca gggcgctgtt cagagtgttg gagaccccga 1380
cctgtgaccc aagtgtaacg ttatgggtcg ttggacaggg cgctgttcag agtgttggag 1440
accccgacct gtgacccaag tgtaacgtcc gtagtggaga caaagcaact ataaaagatt 1500
gttggagacc ccgacctgtg acccaagtgt aacgtccgta gtggagacaa agcaactata 1560
aaagattata tattagatct gcaacctgaa acaactgacc gtccgtagtg gagacaaagc 1620
aactataaaa gattatatat tagatctgca acctgaaaca actgacctac actgctatga 1680
gcaattaggt gacagctcag tatatattag atctgcaacc tgaaacaact gacctacact 1740
gctatgagca attaggtgac agctcagatg aggaggatac agatggtgtg gaccggccag 1800
cctacactgc tatgagcaat taggtgacag ctcagatgag gaggatacag atggtgtgga 1860
ccggccagat ggacaagcag aacaagccac aagcaattac gatgaggagg atacagatgg 1920
tgtggaccgg ccagatggac aagcagaaca agccacaagc aattactaca ttgtgacata 1980
ttgtcacagt tgtgatagca gatggacaag cagaacaagc cacaagcaat tactacattg 2040
tgacatattg tcacagttgt gatagcacac tacggctcgt aattcatagc actgcgacgg 2100
ctacattgtg acatattgtc acagttgtga tagcacacta cggctcgtaa ttcatagcac 2160
tgcgacggac cttcgtactc tacagcaacg tatgttgggc acactacggc tcgtaattca 2220
tagcactgcg acggaccttc gtactctaca gcaacgtatg ttgggcacat tacaagttgt 2280
gtgccccggc tgtgcacggc accttcgtac tctacagcaa cgtatgttgg gcacattaca 2340
agttgtgtgc cccggctgtg cacggctata aacaaccctg caatggagga ccctgaaggt 2400
acattacaag ttgtgtgccc cggctgtgca cggctataaa caaccctgca atggaggacc 2460
ctgaaggtac agagggcgaa agggagggat gtacaggctg ctataaacaa ccctgcaatg 2520
gaggaccctg aaggtacaga gggcgaaagg gagggatgta caggctggtt tgaagtagag 2580
gcaataatag aaaaacaaac ggtacagagg gcgaaaggga gggatgtaca ggctggtttg 2640
aagtagaggc aataatagaa aaacaaacag gagataacat ttcagaggac gaggatgaaa 2700
ggtttgaagt agaggcaata atagaaaaac aaacaggaga taacatttca gaggacgagg 2760
atgaaacgta atatgatagt ggaacagatc taatagattt caggagataa catttcagag 2820
gacgaggatg aaacgtaata tgatagtgga acagatctaa tagattttat agatgattca 2880
aatataaata atgaacaggc cgtaatatga tagtggaaca gatctaatag attttataga 2940
tgattcaaat ataaataatg aacaggcaga acatgaggca gcccgggcat tgtttacgta 3000
tagattttat agatgattca aatataaata atgaacaggc agaacatgag gcagcccggg 3060
cattgtttac gtaacaggaa ggggaggatg attatagatg attcaaatat aaataatgaa 3120
caggcagaac atgaggcagc ccgggcattg tttacgtaac aggaagggga ggatgattta 3180
ccgtatgtgt cgcagaacat gaggcagccc gggcattgtt tacgtaacag gaaggggagg 3240
atgatttacc gtatgtgtct gcagtaaaac gaaagtttac aagcagtccg gcacaggaag 3300
gggaggatga tttaccgtat gtgtctgcag taaaacgaaa gtttacaagc agtccggaaa 3360
gtgctgggca agatggtgta gaaaaacatg gctgcagtaa aacgaaagtt tacaagcagt 3420
ccggaaagtg ctgggcaaga tggtgtagaa aaacatggta gtccgcgtgc aaaacacatt 3480
tgtgtaaata cagtgctggg caagatggtg tagaaaaaca tggtagtccg cgtgcaaaac 3540
acatttgtgt aaatacagag tgtgttttac caaaacgcaa accatgtcac gacagagtgt 3600
gttttaccaa aacgcaaacc atgtcacgta gaagacagcg gctatggcaa tagtgaagtg 3660
gaagcgcagc agatggcaga ccaggtagac gacgtagaag acagcggcta tggcaatagt 3720
gaagtggaag cgcagcagat ggcagaccag gtagacgggc aaaatggcga ctggcaaagt 3780
aacagtagtc atggaagcgc agcagatggc agaccaggta gacgggcaaa atggcgactg 3840
gcaaagtaac agtagtcaat caagtggggt gggggctagt aattcagatg tcgggcaaaa 3900
tggcgactgg caaagtaaca gtagtcaatc aagtggggtg ggggctagta attcagatgt 3960
aagttgtact agtatagagg acaatgagga acaatcaagt ggggtggggg ctagtaattc 4020
agatgtaagt tgtactagta tagaggacaa tgaggaaaat agtaatagaa cgctaaaaag 4080
catacaaaat agtaagttgt actagtatag aggacaatga ggaaaatagt aatagaacgc 4140
taaaaagcat acaaaatatt atgtgcgaaa atagcataaa aacaactgta tatagtaata 4200
gaacgctaaa aagcatacaa aatattatgt gcgaaaatag cataaaaaca actgtattat 4260
ttaaatttaa agaaacatat ggtgttagct tttatgtgcg aaaatagcat aaaaacaact 4320
gtattattta aatttaaaga aacatatggt gttagcttta tggaattagt aagaccattt 4380
aaaagtaata gttaaattta aagaaacata tggtgttagc tttatggaat tagtaagacc 4440
atttaaaagt aatagaagta gttgtacaga ttggtgtatt ataggaatgg ggaagtagtt 4500
gtacagattg gtgtattata ggaatgggag taacaccatc agttgcagaa ggattaaaag 4560
tattaataca gccctatagc atatcgtacc atgggagtaa caccatcagt tgcagaagga 4620
ttaaaagtat taatacagcc ctatagcata tcgtaccatt tgcaatgttt aacatgtgac 4680
agaggcgtgc taagtattaa tacagcccta tagcatatcg taccatttgc aatgtttaac 4740
atgtgacaga ggcgtgctta tactgctgct aattaggttt aaatgtggaa attgcaatgt 4800
ttaacatgtg acagaggcgt gcttatactg ctgctaatta ggtttaaatg tggaaaaaac 4860
agattaacag tgtccaaact aatgtcacag caaaacagat taacagtgtc caaactaatg 4920
tcacagctgt taaatatacc agaaacacat atggtaatag aaccaccaaa attacgaagt 4980
gctacctgtg ccagctgtta aatataccag aaacacatat ggtaatagaa ccaccaaaat 5040
tacgaagtgc tacctgtgca ttatattggt atagaacagg tttgtctaat atagaaccac 5100
caaaattacg aagtgctacc tgtgcattat attggtatag aacaggtttg tctaatatta 5160
gtgaggtata tggtaccacc ccagaatgga tgcattatat tggtatagaa caggtttgtc 5220
taatattagt gaggtatatg gtaccacccc agaatggata gaacaacaaa cagtattaca 5280
gcatagcttt gttagtgagg tatatggtac caccccagaa tggatagaac aacaaacagt 5340
attacagcat agctttgaca atagcatatt cgattttgga gaaatggtgc atagaacaac 5400
aaacagtatt acagcatagc tttgacaata gcatattcga ttttggagaa atggtgcaat 5460
gggcatatga tcatgatata acagatgata ggacaatagc atattcgatt ttggagaaat 5520
ggtgcaatgg gcatatgatc atgatataac agatgatagt gacatagcat ataaatcgta 5580
acagttagca gcaatgggca tatgatcatg atataacaga tgatagtgac atagcatata 5640
aatcgtaaca gttagcagat gtaaatagca cgtatgcagc attatgggca tatgatcatg 5700
atataacaga tgatagtgac atagcatata aatcgtaaca gttagcagat gtaaatagca 5760
cgtatgcagc attcctaaaa agcgtgacat agcatataaa tcgtaacagt tagcagatgt 5820
aaatagcacg tatgcagcat tcctaaaaag caattcgcaa gcaaaaatag taaaggactg 5880
tggtgacata gcatataaat cgtaacagtt agcagatgta aatagcacgt atgcagcatt 5940
cctaaaaagc aattcgcaag caaaaatagt aaaggactgt gcatgtaaat agcacgtatg 6000
cagcattcct aaaaagcaat tcgcaagcaa aaatagtaaa ggactgtgca accatgtgta 6060
gacattataa acgggcagaa agtaaaaagc aattcgcaag caaaaatagt aaaggactgt 6120
gcaaccatgt gtagacatta taaacgggca gaaagaaaac atatgaatat tggacaatgg 6180
atgcaattcg caagcaaaaa tagtaaagga ctgtgcaacc atgtgtagac attataaacg 6240
ggcagaaaga aaacatatga atattggaca atggatacag tacaaccatg tgtagacatt 6300
ataaacgggc agaaagaaaa catatgaata ttggacaatg gatacagtat agatgtgata 6360
gaatagatga tggtggagat tggaaaacat atgaatattg gacaatggat acagtataga 6420
tgtgatagaa tagatgatgg tggagattgg aggcctatag taagattttt aagatatcaa 6480
gaatagatgt gatagaatag atgatggtgg agattggagg cctatagtaa gatttttaag 6540
atatcaagac atagaattta cagccttttt agacgcattt aaggaggcct atagtaagat 6600
ttttaagata tcaagacata gaatttacag cctttttaga cgcatttaaa aaatttttaa 6660
aaggtatacc taaaaaaaat tggacataga atttacagcc tttttagacg catttaaaaa 6720
atttttaaaa ggtataccta aaaaaaattg tttagtatta tatggacctg caaacacagg 6780
aagcatttaa aaaattttta aaaggtatac ctaaaaaaaa ttgtttagta ttatatggac 6840
ctgcaaacac aggaaaatca tattttggaa tgagtttaat aaaaattttt aaaaggtata 6900
cctaaaaaaa attgtttagt attatatgga cctgcaaaca caggaaaatc atattttgga 6960
atgagtttaa ttaggttctt gtttagtatt atatggacct gcaaacacag gaaaatcata 7020
ttttggaatg agtttaatta ggttcttaag tggatgtgta atatcctatg taaactcaaa 7080
aatcatattt tggaatgagt ttaattaggt tcttaagtgg atgtgtaata tcctatgtaa 7140
actcaaaaag ccatttttgg ctacaaccat taacagcgta taagtggatg tgtaatatcc 7200
tatgtaaact caaaaagcca tttttggcta caaccattaa cagcgtaaaa agtgggtatg 7260
atagatgatg taacacctat caaaaagcca tttttggcta caaccattaa cagcgtaaaa 7320
agtgggtatg atagatgatg taacacctat atgttggaca tatatagatg attatatgag 7380
aaagccattt ttggctacaa ccattaacag cgtaaaaagt gggtatgata gatgatgtaa 7440
cacctatatg ttggacatat atagatgatt atatgagaaa cagcgtaaaa agtgggtatg 7500
atagatgatg taacacctat atgttggaca tatatagatg attatatgag aacgtaactg 7560
gatggaaatg agcaaaagtg ggtatgatag atgatgtaac acctatatgt tggacatata 7620
tagatgatta tatgagaacg taactggatg gaaatgatat atcagtagat gtatgttgga 7680
catatataga tgattatatg agaacgtaac tggatggaaa tgatatatca gtagatgtaa 7740
agcatagagc cttagtacaa ataaacgtac ccgtaactgg atggaaatga tatatcagta 7800
gatgtaaagc atagagcctt agtacaaata aacgtaccac cattaatttt aacaacaaat 7860
acaacgtaag gtaaagcata gagccttagt acaaataaac gtaccaccat taattttaac 7920
aacaaataca acgtaaggaa cagatcctag gtggccatat ttacatagta gcaccattaa 7980
ttttaacaac aaatacaacg taaggaacag atcctaggtg gccatattta catagtagat 8040
tggttgtgtt tcatttcaaa aacccatttc cggaacagat cctaggtggc catatttaca 8100
tagtagattg gttgtgtttc atttcaaaaa cccatttcca tttgatgaaa atggcaatcc 8160
tatatatgaa agattggttg tgtttcattt caaaaaccca tttccatttg atgaaaatgg 8220
caatcctata tatgaaatta acaacgaaaa ttggaaatcc tttttctcaa gccatttcca 8280
tttgatgaaa atggcaatcc tatatatgaa attaacaacg aaaattggaa atcctttttc 8340
tcaaggacgt ggtgcaaatt agatttaata ccatttgatg aaaatggcaa tcctatatat 8400
gaaattaaca acgaaaattg gaaatccttt ttctcaagga cgtggtgcaa attagattta 8460
atacaggaag tctcaaggac gtggtgcaaa ttagatttaa tacaggaaga ggacaaggaa 8520
aacgatggag tcgataccgg cacgtttaac gtaagtgcag gaaaaaatac aggacgtggt 8580
gcaaattaga tttaatacag gaagaggaca aggaaaacga tggagtcgat accggcacgt 8640
ttaacgtaag tgcaggaaaa aatactagag gacgtggtgc aaattagatt taatacagga 8700
agaggacaag gaaaacgatg gagtcgatac cggcacgttt aacgtaagtg caggaaaaaa 8760
tactagatca ggacaaggaa aacgatggag tcgataccgg cacgtttaac gtaagtgcag 8820
gaaaaaatac tagatctata cgaagctgat agtaatgacc taaacgcacc gtttaacgta 8880
agtgcaggaa aaaatactag atctatacga agctgatagt aatgacctaa acgcacaaat 8940
tgaacattgg aaattgactc gaatggaatc tatacgaagc tgatagtaat gacctaaacg 9000
cacaaattga acattggaaa ttgactcgaa tggaatgtgt tttgttttac aaagcaaagg 9060
aactgggaac acaaattgaa cattggaaat tgactcgaat ggaatgtgtt ttgttttaca 9120
aagcaaagga actgggaata actcatatag gccaccaggt ggtgccaaat tgaacattgg 9180
aaattgactc gaatggaatg tgttttgttt tacaaagcaa aggaactggg aataactcat 9240
ataggccacc aggtggtgcc accaatgtgt tttgttttac aaagcaaagg aactgggaat 9300
aactcatata ggccaccagg tggtgccacc aatggcagtg tctaaggcaa aggcctgcca 9360
agctaataac tcatataggc caccaggtgg tgccaccaat ggcagtgtct aaggcaaagg 9420
cctgccaagc tattgaacta caattggcat tggaggcatt aaacaatggc agtgtctaag 9480
gcaaaggcct gccaagctat tgaactacaa ttggcattgg aggcattaaa caaaacacaa 9540
tatagcacag atggatggac attacttgaa ctacaattgg cattggaggc attaaacaaa 9600
acacaatata gcacagatgg atggacatta caacaaacaa gtctagaaat gtggcgtgca 9660
gaacccaaaa cacaatatag cacagatgga tggacattac aacaaacaag tctagaaatg 9720
tggcgtgcag aaccacaaaa atactttaaa aaacatgggt atacacaaca aacaagtcta 9780
gaaatgtggc gtgcagaacc acaaaaatac tttaaaaaac atgggtatac aataacagtg 9840
caatacgata atgataaaaa caataccaca aaaatacttt aaaaaacatg ggtatacaat 9900
aacagtgcaa tacgataatg ataaaaacaa tactatggat tatacaaact ggaaggaaat 9960
ttatttaaca gtgcaatacg ataatgataa aaacaatact atggattata caaactggaa 10020
ggaaatttat ttacttggtg agtgtgaatg tacaattgta gaaggctatg gattatacaa 10080
actggaagga aatttattta cttggtgagt gtgaatgtac aattgtagaa ggacaagtag 10140
attactatgg gttatattat tggtgtactt ggtgagtgtg aatgtacaat tgtagaagga 10200
caagtagatt actatgggtt atattattgg tgtgatggag aaaaaatata ttttgtaaaa 10260
tttagggaca agtagattac tatgggttat attattggtg tgatggagaa aaaatatatt 10320
ttgtaaaatt tagtaacgcg taaaagcaat attgtgtaac aggagtgtga tggagaaaaa 10380
atatattttg taaaatttag taacgcgtaa aagcaatatt gtgtaacagg agtatgggaa 10440
gtacatgtgg gtggtcaggt aattggtaac gcgtaaaagc aatattgtgt aacaggagta 10500
tgggaagtac atgtgggtgg tcaggtaatt gtttgtcctg catctgtatc tagtaacgaa 10560
gtatcgtatg ggaagtacat gtgggtggtc aggtaattgt ttgtcctgca tctgtatcta 10620
gtaacgaagt atccactact gaaactgctg tccacctcgt aaccgtgttt gtcctgcatc 10680
tgtatctagt aacgaagtat ccactactga aactgctgtc cacctcgtaa ccgaaacctc 10740
caagacctcc gcagtgtccg tgggtatcca ctactgaaac tgctgtccac ctcgtaaccg 10800
aaacctccaa gacctccgca gtgtccgtgg gtgccaaaga cacacaccta caaccaccac 10860
agaaagaaac ctccaagacc tccgcagtgt ccgtgggtgc caaagacaca cacctacaac 10920
caccacagaa acgacgacga ccagacgtca cagactccag aaacaaaaga cacacaccta 10980
caaccaccac agaaacgacg acgaccagac gtcacagact ccagaaacac caagtacccc 11040
aacaaccttt tgcggggaca acaataccaa gtaccccaac aaccttttgc ggggacaaca 11100
atccgtggac agtactacac ggggactcgt cactgcaact gagtgcacaa acaaaggacg 11160
ggttgcaatc cgtggacagt actacacggg gactcgtcac tgcaactgag tgcacaaaca 11220
aaggacgggt tgcacataca acttgtactg cacctataat acacccactg caactgagtg 11280
cacaaacaaa ggacgggttg cacatacaac ttgtactgca cctataatac acctaaaagg 11340
tgatcctaat agtttaaaat gtttagcaca tacaacttgt actgcaccta taatacacct 11400
aaaaggtgat cctaatagtt taaaatgttt aagatatagg gtaaaaacac ataaaagttt 11460
gtatgcctaa aaggtgatcc taatagttta aaatgtttaa gatatagggt aaaaacacat 11520
aaaagtttgt atgttcaaat ttcatctacc tggcattgga ccagtgatat agggtaaaaa 11580
cacataaaag tttgtatgtt caaatttcat ctacctggca ttggaccagt aatgaatgta 11640
caaataataa actaggtatt gtaacgttca aatttcatct acctggcatt ggaccagtaa 11700
tgaatgtaca aataataaac taggtattgt aacaataacg tacagtgatg aaacacaacg 11760
tcaacaatga atgtacaaat aataaactag gtattgtaac aataacgtac agtgatgaaa 11820
cacaacgtca acaattttta aaaactgtta aaataccaaa tactgacaat aacgtacagt 11880
gatgaaacac aacgtcaaca atttttaaaa actgttaaaa taccaaatac tgtgcaagtt 11940
atacaaggtg tcatgtcatt gtgatcaatt tttaaaaact gttaaaatac caaatactgt 12000
gcaagttata caaggtgtca tgtcattgtg atatttgtac atatgtatat atgtatatgt 12060
gtatggtgca agttatacaa ggtgtcatgt cattgtgata tttgtacata tgtatatatg 12120
tatatgtgta tggtaaacac ccaacacaag ccaatattgc tgctatattt gtacatatgt 12180
atatatgtat atgtgtatgg taaacaccca acacaagcca atattgctgc tattgtgtat 12240
atataacaat gttaggatta tttgtggtaa acacccaaca caagccaata ttgctgctat 12300
tgtgtatata taacaatgtt aggattattt gtattttgtt ttattttgct tatggtgttt 12360
tgtgcttgtg tatatataac aatgttagga ttatttgtat tttgttttat tttgcttatg 12420
gtgttttgtg cagtgcttag gccgctcttg ctatctatat cggtggtatt ttgttttatt 12480
ttgcttatgg tgttttgtgc agtgcttagg ccgctcttgc tatctatatc ggtgtcgtag 12540
caggtgttgg tgctggtgct tttgcgcagt gcttaggccg ctcttgctat ctatatcggt 12600
gtcgtagcag gtgttggtgc tggtgctttt gctatgggta tctattgggt caccatttaa 12660
agtgtcggtg tcgtagcagg tgttggtgct ggtgcttttg ctatgggtat ctattgggtc 12720
accatttaaa gtgttttttt tgtacctact gtttttatat tttcctatgg gtatctattg 12780
ggtcaccatt taaagtgttt tttttgtacc tactgttttt atattttcca atgttttgta 12840
ttcactgtcc gtaacagtat ttggcttttt tgtacctact gtttttatat tttccaatgt 12900
tttgtattca ctgtccgtaa cagtatttgg cacaactgca ataactgtac atgtagattg 12960
gctactgttt tgtattcact gtccgtaaca gtatttggca caactgcaat aactgtacat 13020
gtagattggc taccgtaata tcgtaaaaat atactttttc actttggcac aactgcaata 13080
actgtacatg tagattggct accgtaatat cgtaaaaata tactttttca cttttgtagt 13140
ttgtctaata aatactttta tatttaccgt aatatcgtaa aaatatactt tttcactttt 13200
gtagtttgtc taataaatac ttttatattt tttaatagct tgtcgcaatg agatacagac 13260
ggtctttgta gtttgtctaa taaatacttt tatatttttt aatagcttgt cgcaatgaga 13320
tacagacggt ctacacggca caaacgtgct tctgcaacac agctataata gcttgtcgca 13380
atgagataca gacggtctac acggcacaaa cgtgcttctg caacacagct atatcaaacc 13440
gtaaaagcct ctggcacctg ccccctacac ggcacaaacg tgcttctgca acacagctat 13500
atcaaaccgt aaaagcctct ggcacctgcc cccccgatgt tattcctaaa gtggaaggca 13560
caacttctgc aacacagcta tatcaaaccg taaaagcctc tggcacctgc ccccccgatg 13620
ttattcctaa agtggaaggc acaactattg cagatcaaca aaccgtaaaa gcctctggca 13680
cctgcccccc cgatgttatt cctaaagtgg aaggcacaac tattgcagat caacttttaa 13740
aatatggcag cctaggggtt gcagatcaac ttttaaaata tggcagccta ggggtgtttt 13800
ttggaggttt gggtataggt acaggtgcag gctctggtgg tagggcaggc tatgtgccag 13860
gggtgttttt tggaggtttg ggtataggta caggtgcagg ctctggtggt agggcaggct 13920
atgtgccatt gtccactcgt cctcccacta gtagtattag gtgcaggctc tggtggtagg 13980
gcaggctatg tgccattgtc cactcgtcct cccactagta gtattaccac gtccaccatt 14040
cgtccccctg taactgtagc cattgtccac tcgtcctccc actagtagta ttaccacgtc 14100
caccattcgt ccccctgtaa ctgtagaacc cattggtccc ttagaaccat ctatagttcc 14160
acgtccacca ttcgtccccc tgtaactgta gaacccattg gtcccttaga accatctata 14220
gtttctatga tagaagaaac aacatttatt gagtctggga acccattggt cccttagaac 14280
catctatagt ttctatgata gaagaaacaa catttattga gtctggcgca cctgctccat 14340
ctattccatc agcaacagct atgatagaag aaacaacatt tattgagtct ggcgcacctg 14400
ctccatctat tccatcagca acagggtttg atgttacaac atctgcaaat aatactccgg 14460
gtttgatgtt acaacatctg caaataatac tcctgcaata attaatgtaa catctatagg 14520
tgaatcatct gtacaatcag tttctacaca tttaaatccc tgcaataatt aatgtaacat 14580
ctataggtga atcatctgta caatcagttt ctacacattt aaatcctaca ttcactgaac 14640
catctataat acagccccat catctgtaca atcagtttct acacatttaa atcctacatt 14700
cactgaacca tctataatac agcccccggc acctgcagaa gcatctggtc atgtattgcc 14760
tacattcact gaaccatcta taatacagcc cccggcacct gcagaagcat ctggtcatgt 14820
attgttttct agtccaacta ttagtacaca cacctatgcc ccggcacctg cagaagcatc 14880
tggtcatgta ttgttttcta gtccaactat tagtacacac acctatgaag aaatccctat 14940
ggatacattt gttacctcgt tttctagtcc aactattagt acacacacct atgaagaaat 15000
ccctatggat acatttgtta cctctactga cagcagcagt gtaacaagta gtacacctta 15060
tgaagaaatc cctatggata catttgttac ctctactgac agcagcagtg taacaagtag 15120
tacacctatt ccagggtctc gccctacgac tgaagaaatc cctatggata catttgttac 15180
ctctactgac agcagcagtg taacaagtag tacacctatt ccagggtctc gccctacgac 15240
acgccttggt actgacagca gcagtgtaac aagtagtaca cctattccag ggtctcgccc 15300
tacgacacgc cttggtttat atagccgtgc cacacaacag gttaaggtag ctattccagg 15360
gtctcgccct acgacacgcc ttggtttata tagccgtgcc acacaacagg ttaaggtagt 15420
cgaccctgct tttatgtcat caccacagaa ttatatagcc gtgccacaca acaggttaag 15480
gtagtcgacc ctgcttttat gtcatcacca cagaaattag taacatataa caatcctgtt 15540
tttgagggcg agtcgaccct gcttttatgt catcaccaca gaaattagta acatataaca 15600
atcctgtttt tgagggcgtt gatacagatg aaactataat ttttgatcgt attagtaaca 15660
tataacaatc ctgtttttga gggcgttgat acagatgaaa ctataatttt tgatcgttca 15720
caacttttac ctgcaccgga tcctgatttt tcacaacttt tacctgcacc ggatcctgat 15780
tttttagaca ttatagcttt gcataggcct gcattaacct ctcgaagagg tactgttagg 15840
tttagcaggc tagacattat agctttgcat aggcctgcat taacctctcg aagaggtact 15900
gttaggttta gcaggcttgg taataaggcc accctacgta cacgtagtgg acctctcgaa 15960
gaggtactgt taggtttagc aggcttggta ataaggccac cctacgtaca cgtagtggaa 16020
aacaaattgg ggcacgggta cattattatc ggcttggtaa taaggccacc ctacgtacac 16080
gtagtggaaa acaaattggg gcacgggtac attattatca tgatattagt cctatccagc 16140
ctgctgaagt gtggaaaaca aattggggca cgggtacatt attatcatga tattagtcct 16200
atccagcctg ctgaagttca ggaagacata gaattgcaac ctttattacc tcatgatatt 16260
agtcctatcc agcctgctga agttcaggaa gacatagaat tgcaaccttt attaccacag 16320
tctgtgtccc cttacactat taatgatggt caggaagaca tagaattgca acctttatta 16380
ccacagtctg tgtcccctta cactattaat gatggtttgt atgatgtgtc gtaagattct 16440
ttgcagcaac cagtctgtgt ccccttacac tattaatgat ggtttgtatg atgtgtcgta 16500
agattctttg cagcaaccca cgtttcactt accttccaca ctttctaccc tgtatgatgt 16560
gtcgtaagat tctttgcagc aacccacgtt tcacttacct tccacacttt ctacccataa 16620
taatactttc actgtaccta ttaatagtgg acccacgttt cacttacctt ccacactttc 16680
tacccataat aatactttca ctgtacctat taatagtggt attgactttg tatatcaacc 16740
cactatgtcc ccataataat actttcactg tacctattaa tagtggtatt gactttgtat 16800
atcaacccac tatgtccatt gagtcaggtc ctgacattcc attaccttcg ggtattgact 16860
ttgtatatca acccactatg tccattgagt caggtcctga cattccatta ccttcgttac 16920
ccacacatac tccttttgtt cctatagccc ccattgagtc aggtcctgac attccattac 16980
cttcgttacc cacacatact ccttttgttc ctatagcccc tacagctcca tctacatcta 17040
ttattgttga cgttacccac acatactcct tttgttccta tagcccctac agctccatct 17100
acatctatta ttgttgatgg tacagatttt attttacatc ctagttattt ccttttgttc 17160
ctatagcccc tacagctcca tctacatcta ttattgttga tggtacagat tttattttac 17220
atcctagtta ttttttacta cgtcgcaggc gcccctacag ctccatctac atctattatt 17280
gttgatggta cagattttat tttacatcct agttattttt tactacgtcg caggcgtaaa 17340
cgttttccat ggtacagatt ttattttaca tcctagttat tttttactac gtcgcaggcg 17400
taaacgtttt ccatattttt ttacagatgt ccgtgtggcg gcctagtgag ttttatttta 17460
catcctagtt attttttact acgtcgcagg cgtaaacgtt ttccatattt ttttacagat 17520
gtccgtgtgg cggcctagtg aggccacatc ctagttattt tttactacgt cgcaggcgta 17580
aacgttttcc atattttttt acagatgtcc gtgtggcggc ctagtgaggc cactgtgtac 17640
ctgcctcgca ggcgtaaacg ttttccatat ttttttacag atgtccgtgt ggcggcctag 17700
tgaggccact gtgtacctgc ctcctgtacc tgtctctaag gttgttttta cagatgtccg 17760
tgtggcggcc tagtgaggcc actgtgtacc tgcctcctgt acctgtctct aaggttgtaa 17820
gcactgatga gtatgtagat gtccgtgtgg cggcctagtg aggccactgt gtacctgcct 17880
cctgtacctg tctctaaggt tgtaagcact gatgagtatg tgtctcgcac aagcatccgt 17940
gtggcggcct agtgaggcca ctgtgtacct gcctcctgta cctgtctcta aggttgtaag 18000
cactgatgag tatgtgtctc gcacaagcat ctattatgtg gcggcctagt gaggccactg 18060
tgtacctgcc tcctgtacct gtctctaagg ttgtaagcac tgatgagtat gtgtctcgca 18120
caagcatagg ccactgtgta cctgcctcct gtacctgtct ctaaggttgt aagcactgat 18180
gagtatgtgt ctcgcacaag catctattat tcgtaaggca gttctcgcta aggttgtaag 18240
cactgatgag tatgtgtctc gcacaagcat ctattattcg taaggcagtt ctcgattact 18300
aacagtagga catccctatt tttctatagg ttgtaagcac tgatgagtat gtgtctcgca 18360
caagcatcta ttattcgtaa ggcagttctc gattactaac agtaggacat ccctattttt 18420
ctattaacgc acaagcatct attattcgta aggcagttct cgattactaa cagtaggaca 18480
tccctatttt tctattaaaa acaccagtag tggtaatggt aaaaaaggat tactaacagt 18540
aggacatccc tatttttcta ttaaaaacac cagtagtggt aatggtaaaa aagttttagt 18600
tcccaaggtg tctggcctgc aatacagtta aaaacaccag tagtggtaat ggtaaaaaag 18660
ttttagttcc caaggtgtct ggcctgcaat acagggtatt tagaattaaa ttgccggacc 18720
ctaataattt tagttcccaa ggtgtctggc ctgcaataca gggtatttag aattaaattg 18780
ccggacccta ataaatttgg ttttccagat acatcttttt ataacccggg tatttagaat 18840
taaattgccg gaccctaata aatttggttt tccagataca tctttttata acccagaaac 18900
ccaaaggttg gtgtgggcct gtacaggttg gttttccaga tacatctttt tataacccag 18960
aaacccaaag gttggtgtgg gcctgtacag gcttggaaat tggtagggga cagcctttag 19020
gtgtggggct tggaaattgg taggggacag cctttaggtg tgggtattag tgggcatcct 19080
ttattaaaca agtttgatga tactgaaacc agtaacaaat cgtatggtgg gtattagtgg 19140
gcatccttta ttaaacaagt ttgatgatac tgaaaccagt aacaaatcgt atggtaaacc 19200
tggtatagat aatagggaat gtttatcttg atgatactga aaccagtaac aaatcgtatg 19260
gtaaacctgg tatagataat agggaatgtt tatctatgga ttataagcag actcagttcg 19320
taattttgta aacctggtat agataatagg gaatgtttat ctatggatta taagcagact 19380
cagttcgtaa ttttaggcgt aaaacctcct ataggtgaac attggggggg aatgtttatc 19440
tatggattat aagcagactc agttcgtaat tttaggcgta aaacctccta taggtgaaca 19500
ttggggtaag ggaacccctt gtaatctatg gattataagc agactcagtt cgtaatttta 19560
ggcgtaaaac ctcctatagg tgaacattgg ggtaagggaa ccccttgtaa taataattca 19620
ggaaaaggcg taaaacctcc tataggtgaa cattggggta agggaacccc ttgtaataat 19680
aattcaggaa atcctgggga ttgtcctccc ctacagctca ttaacgtaag ggaacccctt 19740
gtaataataa ttcaggaaat cctggggatt gtcctcccct acagctcatt aacagtgtaa 19800
tacaggatgg ggacatggta gatacatcct ggggattgtc ctcccctaca gctcattaac 19860
agtgtaatac aggatgggga catggtagat acaggatttg gttgcatgga ttttaatacc 19920
ttgcaacagt gtaatacagg atggggacat ggtagataca ggatttggtt gcatggattt 19980
taataccttg caagctagta aaagtgatgt gcccattgat atatgcagga tttggttgca 20040
tggattttaa taccttgcaa gctagtaaaa gtgatgtgcc cattgatata tgtagcagtg 20100
tatgtaagta tccagattat ttgcagcaag ctagtaaaag tgatgtgccc attgatatat 20160
gtagcagtgt atgtaagtat ccagattatt tgcaaatggc tagcgagcca tatggtgaca 20220
gtttggtagc agtgtatgta agtatccaga ttatttgcaa atggctagcg agccatatgg 20280
tgacagtttg ttcttttttc ttagacgtga gcaaatgttt gttagtggct agcgagccat 20340
atggtgacag tttgttcttt tttcttagac gtgagcaaat gtttgttaga cactttttta 20400
atagggccgg taccttaggt gacccgacac ttttttaata gggccggtac cttaggtgac 20460
cctgtgccag gtgatttata tatacaaggg tctaactctg gcaatactgc cactgtacaa 20520
agcaggaccc tgtgccaggt gatttatata tacaagggtc taactctggc aatactgcca 20580
ctgtacaaag cagtgctttt tttcctactc ctagtggttc tatggctaac tctggcaata 20640
ctgccactgt acaaagcagt gctttttttc ctactcctag tggttctatg gtaacctcag 20700
aatcccaatt atttaataaa ccgtagtgct ttttttccta ctcctagtgg ttctatggta 20760
acctcagaat cccaattatt taataaaccg tactggttac aacgtgcgca gggccacaat 20820
aatggcctag tggttctatg gtaacctcag aatcccaatt atttaataaa ccgtactggt 20880
tacaacgtgc gcagggccac aataatggca tatgttgggg gtaacctcag aatcccaatt 20940
atttaataaa ccgtactggt tacaacgtgc gcagggccac aataatggca tatgttgggg 21000
caatcagttg tttgtcacag actggttaca acgtgcgcag ggccacaata atggcatatg 21060
ttggggcaat cagttgtttg tcacagttgt ggataccact cgtagcacta acatgacttt 21120
ggcatatgtt ggggcaatca gttgtttgtc acagttgtgg ataccactcg tagcactaac 21180
atgactttat gtgctgaggt taaaaaggaa agcacatata gtggatacca ctcgtagcac 21240
taacatgact ttatgtgctg aggttaaaaa ggaaagcaca tataaaaatg aaaattttaa 21300
ggaatacctt cgtcatggcg atgtgctgag gttaaaaagg aaagcacata taaaaatgaa 21360
aattttaagg aataccttcg tcatggcgag gaatttgatt tacaatttat ttttcaattg 21420
aaaatgaaaa ttttaaggaa taccttcgtc atggcgagga atttgattta caatttattt 21480
ttcaattgtg caaaattaca ttaacagctg atgttatgac ggcgaggaat ttgatttaca 21540
atttattttt caattgtgca aaattacatt aacagctgat gttatgacat acattcataa 21600
gatggcgtac actattttag tgtgcaaaat tacattaaca gctgatgtta tgacatacat 21660
tcataagatg gcgtacacta ttttagagga ctggcaattt ggccttaccc caccaccgtc 21720
catacattca taagatggcg tacactattt tagaggactg gcaatttggc cttaccccac 21780
caccgtctgc atctttggag gacacataca gatttgtcac aggactggca atttggcctt 21840
accccaccac cgtctgcatc tttggaggac acatacagat ttgtcacttc tactgctata 21900
acttgtcaaa aaaacacacc ctgcatcttt ggaggacaca tacagatttg tcacttctac 21960
tgctataact tgtcaaaaaa acacaccacc taaaggaaag gaagatcctt taaaggacta 22020
cttctactgc tataacttgt caaaaaaaca caccacctaa aggaaaggaa gatcctttaa 22080
aggactatat gttttgggag gtggatttaa aagaaaagtt cacctaaagg aaaggaagat 22140
cctttaaagg actatatgtt ttgggaggtg gatttaaaag aaaagttttc tgcagattta 22200
gatcagtttc ctttaggtag gttttgggag gtggatttaa aagaaaagtt ttctgcagat 22260
ttagatcagt ttcctttagg taggaagttt ttgttacagg cagggctaca ggctaggccc 22320
ggaagttttt gttacaggca gggctacagg ctaggcccaa actaaaacgc cctgcatcat 22380
cggccccacg tacctccaca aagaagaaaa aggttaaaag ggcccaaact aaaacgccct 22440
gcatcatcgg ccccacgtac ctccacaaag aagaaaaagg ttaaaaggta accattgtct 22500
gttgggtaat tgtctgtgtc ggtaaccatt gtctgttggg taattgtctg tgtcatgtat 22560
gtgttgtgta tgtcaaacac aggttaaaag gtaaccattg tttgttatgt aattgttttg 22620
catgtatgtg ttgtgtatgt caaacacagg ttaaaaggta accattgttt gttatgtaat 22680
tgttttgtgt gtgtactgtg ttgtttgcat gttatgtatg aaaggtaacc attgtttgtt 22740
atgtaattgt tttgtgtgtg tactgtgttg tttgcatgtt atgtatgtgt gtgcatgttt 22800
gttgtatttg tcagttcctg gtgtgtgtac tgtgttgttt gcatgttatg tatgtgtgtg 22860
catgtttgtt gtatttgtca gttcctgtat gtatgttttg tgtatgtatt aataaagtac 22920
gtgtgtgcat gtttgttgta tttgtcagtt cctgtatgta tgttttgtgt atgtattaat 22980
aaagtactgt atttactaaa ctatttatag tagtcttatg gtatgtatgt tttgtgtatg 23040
tattaataaa gtactgtatt tactaaacta tttatagtag tcttatgtta tgttatggtt 23100
gcacccacat gagtaacaat ctgtatttac taaactattt atagtagtct tatgttatgt 23160
tatggttgca cccacatgag taacaataca gttgctccta atctattgca tctcctgccc 23220
gttatgttat ggttgcaccc acatgagtaa caatacagtt gctcctaatc tattgcatct 23280
cctgccctac cctgtgtccc ctgccctacc ctgtgtccta acagttgctc ctaatctatt 23340
gcatctcctg ccctaccctg tgtcccctgc cctaccctgt gtcctacttt gttacactac 23400
taattagcct tatactctcc actttgttac actactaatt agccttatac tctccatttt 23460
gtaccatttt gtactatcca ccattttaaa tcctaaccga attcggttgg tcttggcaca 23520
ccattttgta ccattttgta ctatccacca ttttaaatcc taaccgaatt cggttggtct 23580
tggcacaact ttggttgtcc ttggcacagt aacaactatt taaatcctaa ccgaattcgg 23640
ttggtcttgg cacaactttg gttgtccttg gcacagtaac aactattttt atataagttt 23700
cagcaaactg cttaatcctt caactttggt tgtccttggc acagtaacaa ctatttttat 23760
ataagtttca gcaaactgct taatcctttg gtttcctgca gtccactggt ctacacttgt 23820
ttatataagt ttcagcaaac tgcttaatcc tttggtttcc tgcagtccac tggtctacac 23880
ttgttgtccc gcctaaactg acttcttgct gactcacagg ttggtttcct gcagtccact 23940
ggtctacact tgttgtcccg cctaaactga cttcttgctg actcacaggt cctgcagtgc 24000
agctaaacaa tacattgcct tgtcccgcct aaactgactt cttgctgact cacaggtcct 24060
gcagtgcagc taaacaatac attgcctaac attgcatgtt ttaaactgct tttaggcaca 24120
caggtcctgc agtgcagcta aacaatacat tgcctaacat tgcatgtttt aaactgcttt 24180
taggcacata ttttatttaa actttcacgt aactaattac gcctaacatt gcatgtttta 24240
aactgctttt aggcacatat tttatttaaa ctttcacgta actaattaca gtgttggctt 24300
acacaagtac atcctacgcc atattttatt taaactttca cgtaactaat tacagtgttg 24360
gcttacacaa gtacatccta cgccaaatat gtcttgtaaa acatgattaa atactgttac 24420
cagtgttggc ttacacaagt acatcctacg ccaaatatgt cttgtaaaac atgattaaat 24480
actgttactc accaggtgtg cactacacga ccggttacgg aatatgtctt gtaaaacatg 24540
attaaatact gttactcacc aggtgtgcac tacacgaccg gttacggtta ccgtacccac 24600
aaccactttt ttttataatt 24620
<210> 5
<211> 31405
<212> DNA
<213> Artificial Sequence
<220>
<223> 探针型别HPV58
<400> 5
ctaaactata cgtacaaatc ttgtaaaaac tagggtgtaa ccgaaaacgg tctgaccgaa 60
accggtgcat atataaagca gacatttttt ggtaggctac ccgaaaccgg tgcatatata 120
aagcagacat tttttggtag gctactgcag gactatgttc caggacgcag aggagaaacc 180
acggacattg catgatttgt cggtgcatat ataaagcaga cattttttgg taggctactg 240
caggactatg ttccaggacg cagaggagaa accacggaca ttgcatgatt tgtgtcaggc 300
ggctactgca ggactatgtt ccaggacgca gaggagaaac cacggacatt gcatgatttg 360
tgtcaggcgt tggagacatc tgtgcatgaa atcgaattga agaaaccacg gacattgcat 420
gatttgtgtc aggcgttgga gacatctgtg catgaaatcg aattgaacgt agttgacgta 480
aaaaagactt tgcagcgatc cgttggagac atctgtgcat gaaatcgaat tgaacgtagt 540
tgacgtaaaa aagactttgc agcgatctga ggtatatgac tttgtatttg cagatttaag 600
acgtagttga cgtaaaaaag actttgcagc gatctgaggt atatgacttt gtatttgcag 660
atttaagaat agtgtataga gatggaaatc catttgcagt ctgaggtata tgactttgta 720
tttgcagatt taagaatagt gtatagagat ggaaatccat ttgcagtatg taaagtgtgc 780
ttacgattgc tatctaaaat gaatagtgta tagagatgga aatccatttg cagtatgtaa 840
agtgtgctta cgattgctat ctaaaataag tgagtataga cattataatt attcgctata 900
tatgtaaagt gtgcttacga ttgctatcta aaataagtga gtatagacat tataattatt 960
cgctatatgg agacacatta gaacaaacac taaaaaagtg taagtgagta tagacattat 1020
aattattcgc tatatggaga cacattagaa caaacactaa aaaagtgttt aaatgaaata 1080
ttaattagat gtattatttg ggagacacat tagaacaaac actaaaaaag tgtttaaatg 1140
aaatattaat tagatgtatt atttgtcaaa gaccattgtg tccacaagaa aaaaaaaggc 1200
gtttaaatga aatattaatt agatgtatta tttgtcaaag accattgtgt ccacaagaaa 1260
aaaaaaggca tgtggattta aacaaaaggt ttcataatat tgtcaaagac cattgtgtcc 1320
acaagaaaaa aaaaggcatg tggatttaaa caaaaggttt cataatattt cgggtcgttg 1380
gacagggcgc tgtgcgtcaa agaccattgt gtccacaaga aaaaaaaagg catgtggatt 1440
taaacaaaag gtttcataat atttcgggtc gttggacagg gcgctgtgca gtgtgggcat 1500
gtggatttaa acaaaaggtt tcataatatt tcgggtcgtt ggacagggcg ctgtgcagtg 1560
tgttggagac cccgacgtag acaaacacaa gtgtacataa tatttcgggt cgttggacag 1620
ggcgctgtgc agtgtgttgg agaccccgac gtagacaaac acaagtgtaa cctgtaacaa 1680
cgccatgaga ggaaatttcg ggtcgttgga cagggcgctg tgcagtgtgt tggagacccc 1740
gacgtagaca aacacaagtg taacctgtaa caacgccatg agaggaaaca acccagttgg 1800
agaccccgac gtagacaaac acaagtgtaa cctgtaacaa cgccatgaga ggaaacaacc 1860
caacgctaag agaatatatt ttagatttac atcctaacct gtaacaacgc catgagagga 1920
aacaacccaa cgctaagaga atatatttta gatttacatc ctgaaccaac tgacctattc 1980
tgctatgagc aattaacgct aagagaatat attttagatt tacatcctga accaactgac 2040
ctattctgct atgagcaatt atgtgacagc tcagacgagg atgaaatagg cttggtgaac 2100
caactgacct attctgctat gagcaattat gtgacagctc agacgaggat gaaataggct 2160
tggacgggcc agatggacaa gcacaaccgg ccacagcaat tatgtgacag ctcagacgag 2220
gatgaaatag gcttggacgg gccagatgga caagcacaac cggccacagc taattactac 2280
attgtaactt gttgttacat gtgacagctc agacgaggat gaaataggct tggacgggcc 2340
agatggacaa gcacaaccgg ccacagctaa ttactacatt gtaacttgtt gttacactgg 2400
cttggacggg ccagatggac aagcacaacc ggccacagct aattactaca ttgtaacttg 2460
ttgttacact tgtggcacca cggttcgttt ggacgggcca gatggacaag cacaaccggc 2520
cacagctaat tactacattg taacttgttg ttacacttgt ggcaccacgg ttcgtttgtg 2580
tatcaacagt gctaattact acattgtaac ttgttgttac acttgtggca ccacggttcg 2640
tttgtgtatc aacagtacaa caaccgacgt acgaacccta cagcagctgc ttgtggcacc 2700
acggttcgtt tgtgtatcaa cagtacaaca accgacgtac gaaccctaca gcagctgctt 2760
atgggcacat gtaccattgt gtgccctagc acaacaaccg acgtacgaac cctacagcag 2820
ctgcttatgg gcacatgtac cattgtgtgc cctagctgtg cacagcaata aacaccatct 2880
gcaatggatg tgcttatggg cacatgtacc attgtgtgcc ctagctgtgc acagcaataa 2940
acaccatctg caatggatga ccctgaaggt acaaacgggg taggggcggg ctgtgcacag 3000
caataaacac catctgcaat ggatgaccct gaaggtacaa acggggtagg ggcgggctgt 3060
actggctggt ttgaggtaga agcggtaata gaccctgaag gtacaaacgg ggtaggggcg 3120
ggctgtactg gctggtttga ggtagaagcg gtaatagaac gaagaacagg agataatatt 3180
tcagatgatg agaacgaaga acaggagata atatttcaga tgatgaggac gaaacagcag 3240
acgatagtgg tacagattta atagagttta tagatgattc agtacaaagt tgaggacgaa 3300
acagcagacg atagtggtac agatttaata gagtttatag atgattcagt acaaagtact 3360
acacaggcag aagcagaggc agcccgagcg cagatttaat agagtttata gatgattcag 3420
tacaaagtac tacacaggca gaagcagagg cagcccgagc gttgtttaat gtacaggaag 3480
gggtttaata gagtttatag atgattcagt acaaagtact acacaggcag aagcagaggc 3540
agcccgagcg ttgtttaatg tacaggaagg ggtggacgat atactacaca ggcagaagca 3600
gaggcagccc gagcgttgtt taatgtacag gaaggggtgg acgatataac gtatgtgtgt 3660
gcactaaaac gaaagtttgc agccacaggc agaagcagag gcagcccgag cgttgtttaa 3720
tgtacaggaa ggggtggacg atataacgta tgtgtgtgca ctaaaacgaa agtgttgttt 3780
aatgtacagg aaggggtgga cgatataacg tatgtgtgtg cactaaaacg aaagtttgca 3840
gccgtatcag aaagtgctgt agaggactgt gtgttgttta atgtacagga aggggtggac 3900
gatataacgt atgtgtgtgc actaaaacga aagtttgcag ccgtatcaga aagtgctgta 3960
gaggacaacg tatgtgtgtg cactaaaacg aaagtttgca gccgtatcag aaagtgctgt 4020
agaggactgt gtggaccggg ctgcaaatgt gtgtgtatcg tggaaacagc cgtatcagaa 4080
agtgctgtag aggactgtgt ggaccgggct gcaaatgtgt gtgtatcgtg gaaatataaa 4140
aataaagacg taacacacag aaaacgggac cgggctgcaa atgtgtgtgt atcgtggaaa 4200
tataaaaata aagacgtaac acacagaaaa cgaaaaatta ttgagctaga agacagcgga 4260
tatggctata aaaataaaga cgtaacacac agaaaacgaa aaattattga gctagaagac 4320
agcggatatg gcaatactga agtggaaact gagcagatgg cacaccataa aaataaagac 4380
gtaacacaca gaaaacgaaa aattattgag ctagaagaca gcggatatgg caatactgaa 4440
gtggaaactg agcagatgga cgaaaaatta ttgagctaga agacagcgga tatggcaata 4500
ctgaagtgga aactgagcag atggcacacc aggtagaaag ccaaaatggc gacatggcaa 4560
tactgaagtg gaaactgagc agatggcaca ccaggtagaa agccaaaatg gcgacgcaga 4620
cttaaatgac tcggagtcta gtggggtggg ggaatactga agtggaaact gagcagatgg 4680
cacaccaggt agaaagccaa aatggcgacg cagacttaaa tgactcggag tctagtgggg 4740
tgggggctag ttccaggtag aaagccaaaa tggcgacgca gacttaaatg actcggagtc 4800
tagtggggtg ggggctagtt cagatgtaag cagtgaaacg gatgtagaca gtaaatgact 4860
cggagtctag tggggtgggg gctagttcag atgtaagcag tgaaacggat gtagacagtt 4920
gtaatactgt tccattacaa aatattagta atgttcagat gtaagcagtg aaacggatgt 4980
agacagttgt aatactgttc cattacaaaa tattagtaat attctacata acagtaatac 5040
taaagcaacg ctgttgtaat actgttccat tacaaaatat tagtaatatt ctacataaca 5100
gtaatactaa agcaacgcta ttatataaat tcaaagaagc ttatggagta aggtaatatt 5160
ctacataaca gtaatactaa agcaacgcta ttatataaat tcaaagaagc ttatggagta 5220
agttttatgg aattagttag accatttaaa agtattctac ataacagtaa tactaaagca 5280
acgctattat ataaattcaa agaagcttat ggagtaagtt ttatggaatt agttagacca 5340
tttaaaagtg attattatat aaattcaaag aagcttatgg agtaagtttt atggaattag 5400
ttagaccatt taaaagtgat aaaacaagct gtacagattg gtgtataaca ttatataaat 5460
tcaaagaagc ttatggagta agttttatgg aattagttag accatttaaa agtgataaaa 5520
caagctgtac agattggtgt ataacagggt aagttttatg gaattagtta gaccatttaa 5580
aagtgataaa acaagctgta cagattggtg tataacaggg tatggaataa gtccctccgt 5640
agcagaaagg ttttatggaa ttagttagac catttaaaag tgataaaaca agctgtacag 5700
attggtgtat aacagggtat ggaataagtc cctccgtagc agaaagtttg ttagaccatt 5760
taaaagtgat aaaacaagct gtacagattg gtgtataaca gggtatggaa taagtccctc 5820
cgtagcagaa agtttaaaag tgataaaaca agctgtacag attggtgtat aacagggtat 5880
ggaataagtc cctccgtagc agaaagttta aaagtactaa ttaaacagca aaaacaagct 5940
gtacagattg gtgtataaca gggtatggaa taagtccctc cgtagcagaa agtttaaaag 6000
tactaattaa acagcacagt atatatacac gtttaaaagt actaattaaa cagcacagta 6060
tatatacaca cctacaatgt ttaacgtgtg acagaggaat tatattatta ttgttaatta 6120
gatttaaaaa gtactaatta aacagcacag tatatataca cacctacaat gtttaacgtg 6180
tgacagagga attatattat tattgttaat tagatttaaa tgtagccaca cctacaatgt 6240
ttaacgtgtg acagaggaat tatattatta ttgttaatta gatttaaatg tagcaaaaat 6300
agattaactg tggcaaaatt aatgagtata ttattattgt taattagatt taaatgtagc 6360
aaaaatagat taactgtggc aaaattaatg agtaatttac tatcaattcc tgaaacatgt 6420
atgatttatt attgttaatt agatttaaat gtagcaaaaa tagattaact gtggcaaaat 6480
taatgagtaa tttactatca attcctgaaa catgtagcaa aaatagatta actgtggcaa 6540
aattaatgag taatttacta tcaattcctg aaacatgtat gattatcgag ccaccaaaat 6600
tacgaagtca agcatgtgag taatttacta tcaattcctg aaacatgtat gattatcgag 6660
ccaccaaaat tacgaagtca agcatgtgcc ttatattggt ttagaacagc aatgtcgtaa 6720
tttactatca attcctgaaa catgtatgat tatcgagcca ccaaaattac gaagtcaagc 6780
atgtgcctta tattggttta gaacagcaat gtcaaatgat tatcgagcca ccaaaattac 6840
gaagtcaagc atgtgcctta tattggttta gaacagcaat gtcaaatata agtgatgtgc 6900
aagggacaac accagatatc gagccaccaa aattacgaag tcaagcatgt gccttatatt 6960
ggtttagaac agcaatgtca aatataagtg atgtgcaagg gacaacacca gaatggccac 7020
caaaattacg aagtcaagca tgtgccttat attggtttag aacagcaatg tcaaatataa 7080
gtgatgtgca agggacaaca ccagaaccac caaaattacg aagtcaagca tgtgccttat 7140
attggtttag aacagcaatg tcaaatataa gtgatgtgca agggacaaca ccagaatggg 7200
tgccttatat tggtttagaa cagcaatgtc aaatataagt gatgtgcaag ggacaacacc 7260
agaatggata gatagattaa cagtgttaca gcatagctta taagtgatgt gcaagggaca 7320
acaccagaat ggatagatag attaacagtg ttacagcata gctttaatga tgatatattt 7380
gatttaagtg aaatgataca gtgatgtgca agggacaaca ccagaatgga tagatagatt 7440
aacagtgtta cagcatagct ttaatgatga tatatttgat ttaagtgaaa tacaccagaa 7500
tggatagata gattaacagt gttacagcat agctttaatg atgatatatt tgatttaagt 7560
gaaatgatac aatgggcata tgataatgag gatagataga ttaacagtgt tacagcatag 7620
ctttaatgat gatatatttg atttaagtga aatgatacaa tgggcatatg ataatgacat 7680
tacagatgag ctttaatgat gatatatttg atttaagtga aatgatacaa tgggcatatg 7740
ataatgacat tacagatgat agtgacattg catataaatc gtaacaaatg atgatatatt 7800
tgatttaagt gaaatgatac aatgggcata tgataatgac attacagatg atagtgacat 7860
tgcatataaa tcgtaacagt tagcagatga tatatttgat ttaagtgaaa tgatacaatg 7920
ggcatatgat aatgacatta cagatgatag tgacattgca tataaatcgt aacatgatac 7980
aatgggcata tgataatgac attacagatg atagtgacat tgcatataaa tcgtaacagt 8040
tagcagatgt taatagtacg taagcagcat ttttacaatg ggcatatgat aatgacatta 8100
cagatgatag tgacattgca tataaatcgt aacagttagc agatgttaat agtacgtaag 8160
cagcattttt aagagtgaca ttgcatataa atcgtaacag ttagcagatg ttaatagtac 8220
gtaagcagca tttttaagaa gcacgtaaca agcaaaaata gtaaaagact gtggtgacat 8280
tgcatataaa tcgtaacagt tagcagatgt taatagtacg taagcagcat ttttaagaag 8340
cacgtaacaa gcaaaaatag taaaagactg tggatgttaa tagtacgtaa gcagcatttt 8400
taagaagcac gtaacaagca aaaatagtaa aagactgtgg cgttatgtgc agacattata 8460
aaagagcaga aagaagcacg taacaagcaa aaatagtaaa agactgtggc gttatgtgca 8520
gacattataa aagagcagaa aagcgtggta tgacaatggg acaatggata caactgtggc 8580
gttatgtgca gacattataa aagagcagaa aagcgtggta tgacaatggg acaatggata 8640
caaagtaggt gtgaaaaaac aaatgatgga gggtggcgtt atgtgcagac attataaaag 8700
agcagaaaag cgtggtatga caatgggaca atggatacaa agtaggtgtg aaaaaacaaa 8760
tgatggaggt aagcagaaaa gcgtggtatg acaatgggac aatggataca aagtaggtgt 8820
gaaaaaacaa atgatggagg taattggaga ccaatagtac aatttttaag ataaagcgtg 8880
gtatgacaat gggacaatgg atacaaagta ggtgtgaaaa aacaaatgat ggaggtaatt 8940
ggagaccaat agtacaattt ttaagatatc aatacaaagt aggtgtgaaa aaacaaatga 9000
tggaggtaat tggagaccaa tagtacaatt tttaagatat caaaatattg aatttacagc 9060
atttttagtt gcaagtaggt gtgaaaaaac aaatgatgga ggtaattgga gaccaatagt 9120
acaattttta agatatcaaa atattgaatt tacagcattt ttagttgcat ttggtaattg 9180
gagaccaata gtacaatttt taagatatca aaatattgaa tttacagcat ttttagttgc 9240
atttaaacag tttttacaag gtgtaccaaa aattggagac caatagtaca atttttaaga 9300
tatcaaaata ttgaatttac agcattttta gttgcattta aacagttttt acaaggtgta 9360
ccaaaaaaaa gtaaatattg aatttacagc atttttagtt gcatttaaac agtttttaca 9420
aggtgtacca aaaaaaagtt gtatgttact gtgtggccca gcaaatacag ggtaaacagt 9480
ttttacaagg tgtaccaaaa aaaagttgta tgttactgtg tggcccagca aatacaggga 9540
aatcatattt tggaatgagt ttaatacatt ttttgtatgt tactgtgtgg cccagcaaat 9600
acagggaaat catattttgg aatgagttta atacattttt taaaaggcgt aattatttca 9660
tatgtaaatt ccatacaggg aaatcatatt ttggaatgag tttaatacat tttttaaaag 9720
gcgtaattat ttcatatgta aattccaaaa gtcatttttg gttggggaaa tcatattttg 9780
gaatgagttt aatacatttt ttaaaaggcg taattatttc atatgtaaat tccaaaagtc 9840
atttttggtt gcagccatta tcagggaaat catattttgg aatgagttta atacattttt 9900
taaaaggcgt aattatttca tatgtaaatt ccaaaagtca tttttggttg cagccattat 9960
ctttttaaaa ggcgtaatta tttcatatgt aaattccaaa agtcattttt ggttgcagcc 10020
attatcagcg tataaactag gtatgataga tgatgtaact ttaaaaggcg taattatttc 10080
atatgtaaat tccaaaagtc atttttggtt gcagccatta tcagcgtata aactaggtat 10140
gatagatgat gtaacagcct tccaaaagtc atttttggtt gcagccatta tcagcgtata 10200
aactaggtat gatagatgat gtaacagcca taagctggac atatatagat gattatatgc 10260
caaaagtcat ttttggttgc agccattatc agcgtataaa ctaggtatga tagatgatgt 10320
aacagccata agctggacat atatagatga ttatatgaga aagtcatttt tggttgcagc 10380
cattatcagc gtataaacta ggtatgatag atgatgtaac agccataagc tggacatata 10440
tagatgatta tatgagaaac agcgtataaa ctaggtatga tagatgatgt aacagccata 10500
agctggacat atatagatga ttatatgaga acgtaattag atggtaacga catttcaatg 10560
ctaaactagg tatgatagat gatgtaacag ccataagctg gacatatata gatgattata 10620
tgagaacgta attagatggt aacgacattt caatagatgc cataagctgg acatatatag 10680
atgattatat gagaacgtaa ttagatggta acgacatttc aatagatgta aaacataggg 10740
cattagtaca attaaaatga taagctggac atatatagat gattatatga gaacgtaatt 10800
agatggtaac gacatttcaa tagatgtaaa acatagggca ttagtacaat taaaattata 10860
tgagaacgta attagatggt aacgacattt caatagatgt aaaacatagg gcattagtac 10920
aattaaaatg tccaccatta ataatgagaa cgtaattaga tggtaacgac atttcaatag 10980
atgtaaaaca tagggcatta gtacaattaa aatgtccacc attaataatt acctcaaata 11040
caaatgcatt agatggtaac gacatttcaa tagatgtaaa acatagggca ttagtacaat 11100
taaaatgtcc accattaata attacctcaa atacaacgta aggctaaaac atagggcatt 11160
agtacaatta aaatgtccac cattaataat tacctcaaat acaacgtaag gcaaagattc 11220
acgatggcca tatttgcaaa tgtccaccat taataattac ctcaaataca acgtaaggca 11280
aagattcacg atggccatat ttgcacagta gactaacagt atttgaattt aacaatcccc 11340
accattaata attacctcaa atacaacgta aggcaaagat tcacgatggc catatttgca 11400
cagtagacta acagtatttg aatttaacaa tccatttcca cagtagacta acagtatttg 11460
aatttaacaa tccatttcca tttgcgtaaa atggtaatcc agtgtataaa ataaatgatg 11520
aaaattggaa atccttttgt agactaacag tatttgaatt taacaatcca tttccatttg 11580
cgtaaaatgg taatccagtg tataaaataa atgatgaaaa ttggaaatcc tttttctcaa 11640
tccatttcca tttgcgtaaa atggtaatcc agtgtataaa ataaatgatg aaaattggaa 11700
atcctttttc tcaaggacgt ggtgcaaatt accatttcca tttgcgtaaa atggtaatcc 11760
agtgtataaa ataaatgatg aaaattggaa atcctttttc tcaaggacgt ggtgcaaatt 11820
aggcttaata gccatttgcg taaaatggta atccagtgta taaaataaat gatgaaaatt 11880
ggaaatcctt tttctcaagg acgtggtgca aattaggctt aatagaggaa gccatttgcg 11940
taaaatggta atccagtgta taaaataaat gatgaaaatt ggaaatcctt tttctcaagg 12000
acgtggtgca aattaggctt aatagaggaa gaataaatga tgaaaattgg aaatcctttt 12060
tctcaaggac gtggtgcaaa ttaggcttaa tagaggaaga ggacaaggaa aacgatggag 12120
gaaatatctc tcaaggacgt ggtgcaaatt aggcttaata gaggaagagg acaaggaaaa 12180
cgatggagga aatatcagca cgtttaagtg cagtgcagga caaaatccct caaggacgtg 12240
gtgcaaatta ggcttaatag aggaagagga caaggaaaac gatggaggaa atatcagcac 12300
gtttaagtgc agtgcaggac aaaatcctag gacgtggtgc aaattaggct taatagagga 12360
agaggacaag gaaaacgatg gaggaaatat cagcacgttt aagtgcagtg caggacaaaa 12420
tcctagacgg acgtggtgca aattaggctt aatagaggaa gaggacaagg aaaacgatgg 12480
aggaaatatc agcacgttta agtgcagtgc aggacaaaat cctagaggaa gaggacaagg 12540
aaaacgatgg aggaaatatc agcacgttta agtgcagtgc aggacaaaat cctagacata 12600
tacgaagctg ataaaaatga tttaacagag gacaaggaaa acgatggagg aaatatcagc 12660
acgtttaagt gcagtgcagg acaaaatcct agacatatac gaagctgata aaaatgattt 12720
aacatctcag cacgtttaag tgcagtgcag gacaaaatcc tagacatata cgaagctgat 12780
aaaaatgatt taacatcaca aattgaacat tggaaactaa tacgcagcac gtttaagtgc 12840
agtgcaggac aaaatcctag acatatacga agctgataaa aatgatttaa catcacaaat 12900
tgaacattgg aaactaatac gcatggacat atacgaagct gataaaaatg atttaacatc 12960
acaaattgaa cattggaaac taatacgcat ggagtgtgct ataatgtata cagccagaca 13020
aatgggtacg aagctgataa aaatgattta acatcacaaa ttgaacattg gaaactaata 13080
cgcatggagt gtgctataat gtatacagcc agacaaacat cacaaattga acattggaaa 13140
ctaatacgca tggagtgtgc tataatgtat acagccagac aaatgggaat atcacatttg 13200
tgccaccagg tggtgccaca aattgaacat tggaaactaa tacgcatgga gtgtgctata 13260
atgtatacag ccagacaaat gggaatatca catttgtgcc accaggtggt gccgtcatgg 13320
agtgtgctat aatgtataca gccagacaaa tgggaatatc acatttgtgc caccaggtgg 13380
tgccgtcatt ggtagcatca aagactaaag cgtttcgagt gtgctataat gtatacagcc 13440
agacaaatgg gaatatcaca tttgtgccac caggtggtgc cgtcattggt agcatcaaag 13500
actaaagcgt ttcaagatgg gaatatcaca tttgtgccac caggtggtgc cgtcattggt 13560
agcatcaaag actaaagcgt ttcaagtaat tgaactgcaa atggcattag agacatggga 13620
atatcacatt tgtgccacca ggtggtgccg tcattggtag catcaaagac taaagcgttt 13680
caagtaattg aactgcaaat ggcattagag acattacatt ggtagcatca aagactaaag 13740
cgtttcaagt aattgaactg caaatggcat tagagacatt aacgtaatca ccatataaaa 13800
cagatgaatg gacattcaag taattgaact gcaaatggca ttagagacat taacgtaatc 13860
accatataaa acagatgaat ggacattgca acaaacaagc ttagaagtgt ggttgaactg 13920
caaatggcat tagagacatt aacgtaatca ccatataaaa cagatgaatg gacattgcaa 13980
caaacaagct tagaagtgtg gttatcagag ccggacattg caacaaacaa gcttagaagt 14040
gtggttatca gagccacaaa acgtatttaa aaaaaaaggc ataacagtaa ctgtacaata 14100
tgacaatgat aagcaacaaa caagcttaga agtgtggtta tcagagccac aaaacgtatt 14160
taaaaaaaaa ggcataacag taactgtaca atatgacaat gataaagcaa acgagccaca 14220
aaacgtattt aaaaaaaaag gcataacagt aactgtacaa tatgacaatg ataaagcaaa 14280
cacaatggat tatacaaatt ggagtgaaat ataacagtaa ctgtacaata tgacaatgat 14340
aaagcaaaca caatggatta tacaaattgg agtgaaatat atattattga ggaaacaaca 14400
tgtactttgg cagtaactgt acaatatgac aatgataaag caaacacaat ggattataca 14460
aattggagtg aaatatatat tattgaggaa acaacatgta ctttggtagc acacaatgga 14520
ttatacaaat tggagtgaaa tatatattat tgaggaaaca acatgtactt tggtagcagg 14580
agaagttgac tatgtggggt tgtattatat atattattga ggaaacaaca tgtactttgg 14640
tagcaggaga agttgactat gtggggttgt attatataca tggcaatgaa aagacgtatt 14700
ttaaatattt gcaggagaag ttgactatgt ggggttgtat tatatacatg gcaatgaaaa 14760
gacgtatttt aaatatttta aagaggcgta aaaaaagtac tctaaaacac tacatggcaa 14820
tgaaaagacg tattttaaat attttaaaga ggcgtaaaaa aagtactcta aaacacaatt 14880
atgggaggta catgtgggta gtcgggtaat ttaaagaggc gtaaaaaaag tactctaaaa 14940
cacaattatg ggaggtacat gtgggtagtc gggtaattgt atgtcctaca tctataccta 15000
gtgatcaaat ttatgggagg tacatgtggg tagtcgggta attgtatgtc ctacatctat 15060
acctagtgat caaatatcca ctactgaaac tgctgaccca aagaccaccg ttgtatgtcc 15120
tacatctata cctagtgatc aaatatccac tactgaaact gctgacccaa agaccaccga 15180
ggccaccaac aacgaaagta cacaggggac atatccacta ctgaaactgc tgacccaaag 15240
accaccgagg ccaccaacaa cgaaagtaca caggggacaa agcgacgacg actcgattta 15300
ccagactcca ccgaggccac caacaacgaa agtacacagg ggacaaagcg acgacgactc 15360
gatttaccag actccagaga caacacccag tactccacaa agtatacaga acaaagcgac 15420
gacgactcga tttaccagac tccagagaca acacccagta ctccacaaag tatacagact 15480
gcgccgtgga cagtagacca cgaggaggag gagacaacac ccagtactcc acaaagtata 15540
cagactgcgc cgtggacagt agaccacgag gaggaggact acacagtaca actaactgta 15600
catacaaagg ctgcgccgtg gacagtagac cacgaggagg aggactacac agtacaacta 15660
actgtacata caaagggcgg aacgtgtgta gttctaaagt ttcacctatc ggactacaca 15720
gtacaactaa ctgtacatac aaagggcgga acgtgtgtag ttctaaagtt tcacctatcg 15780
tgcatttaaa aggtgaccca aatagtttaa gggcggaacg tgtgtagttc taaagtttca 15840
cctatcgtgc atttaaaagg tgacccaaat agtttaaaat gtttaagata tagattaaaa 15900
ccatttaaag gtgtagttct aaagtttcac ctatcgtgca tttaaaaggt gacccaaata 15960
gtttaaaatg tttaagatat agattaaaac catttaaaga cgtgcattta aaaggtgacc 16020
caaatagttt aaaatgttta agatatagat taaaaccatt taaagactta tactgtaata 16080
tgtcatccac atggcattgg taaaaggtga cccaaatagt ttaaaatgtt taagatatag 16140
attaaaacca tttaaagact tatactgtaa tatgtcatcc acatggcatt ggaccagtaa 16200
aggtgaccca aatagtttaa aatgtttaag atatagatta aaaccattta aagacttata 16260
ctgtaatatg tcatccacat ggcattggac cagtgaaagg tgacccaaat agtttaaaat 16320
gtttaagata tagattaaaa ccatttaaag acttatactg taatatgtca tccacatggc 16380
attggacaat gtttaagata tagattaaaa ccatttaaag acttatactg taatatgtca 16440
tccacatggc attggaccag tgatgacaaa ggtgacaaag taggaatact tatactgtaa 16500
tatgtcatcc acatggcatt ggaccagtga tgacaaaggt gacaaagtag gaattgttac 16560
tgtaacatac acaacggaaa cacaacggga ccagtgatga caaaggtgac aaagtaggaa 16620
ttgttactgt aacatacaca acggaaacac aacgacaact gtttttaaac actgttaaaa 16680
taccacctgt tactgtaaca tacacaacgg aaacacaacg acaactgttt ttaaacactg 16740
ttaaaatacc acccactgtg caaataagta ctggtgttat gtcattggac aactgttttt 16800
aaacactgtt aaaataccac ccactgtgca aataagtact ggtgttatgt cattgtaatt 16860
gtattgtaca attactgtat gtaaaccccc actgtgcaaa taagtactgg tgttatgtca 16920
ttgtaattgt attgtacaat tactgtatgt aaaccacaag ccaatatgtg ctgctaagtg 16980
tatatacgta attgtattgt acaattactg tatgtaaacc acaagccaat atgtgctgct 17040
aagtgtatat acaatgatat tacctatttt tgttgtttgt tttataccca caagccaata 17100
tgtgctgcta agtgtatata caatgatatt acctattttt gttgtttgtt ttatactgtt 17160
tttcgtattg tgcatttttt tgcggccatg atattaccta tttttgttgt ttgttttata 17220
ctgtttttcg tattgtgcat ttttttgcgg ccattggtgc tatctatttc tatatcgtat 17280
tggttgcctg tttttcgtat tgtgcatttt tttgcggcca ttggtgctat ctatttctat 17340
atcgtattgg ttgctggtgt tggtgttgct gctttgggtg tctgtggtgt ttttcgtatt 17400
gtgcattttt ttgcggccat tggtgctatc tatttctata tcgtattggt tgctggtgtt 17460
ggtgttgctg ctttgggtgt ctgtggggct ggtgttggtg ttgctgcttt gggtgtctgt 17520
ggggtcggct ctacgaattt ttttctgtta cttaatattt ttatatatac caatgatgtg 17580
tattaatgct ggtgttggtg ttgctgcttt gggtgtctgt ggggtcggct ctacgaattt 17640
ttttctgtta cttaatattt ttatatatac caatgatgtg tattaatggg gtcggctcta 17700
cgaatttttt tctgttactt aatattttta tatataccaa tgatgtgtat taattttccg 17760
taacaatact taacccaaca agactaaaat atttttatat ataccaatga tgtgtattaa 17820
ttttccgtaa caatacttaa cccaacaaga ctaactgtat actggttctg cacatggtgg 17880
tatggtattt tccgtaacaa tacttaaccc aacaagacta actgtatact ggttctgcac 17940
atggtggtat ggtattgtaa atatttactg ttgtgtgtgt tgtttttact gtatactggt 18000
tctgcacatg gtggtatggt attgtaaata tttactgttg tgtgtgttgt ttttattatt 18060
tttatacatt tactaataaa tacttttatt gtaaatattt actgttgtgt gtgttgtttt 18120
tattattttt atacatttac taataaatac ttttatattt ttagcactgt cttattatga 18180
gacacaatat tatttttata catttactaa taaatacttt tatattttta gcactgtctt 18240
attatgagac acaaacggtc tacaaggcgc aagcgtgcat ctgctacata cttttatatt 18300
tttagcactg tcttattatg agacacaaac ggtctacaag gcgcaagcgt gcatctgcta 18360
cacaacttta ccaaaccgta aaggcctcat atttttagca ctgtcttatt atgagacaca 18420
aacggtctac aaggcgcaag cgtgcatctg ctacacaact ttaccaaacc gtaaaggcct 18480
caggcaccag acacaaacgg tctacaaggc gcaagcgtgc atctgctaca caactttacc 18540
aaaccgtaaa ggcctcaggc acctgcccac ctgatgttat aaacggtcta caaggcgcaa 18600
gcgtgcatct gctacacaac tttaccaaac cgtaaaggcc tcaggcacct gcccacctga 18660
tgttataccc aaagttgaag cacaacttta ccaaaccgta aaggcctcag gcacctgccc 18720
acctgatgtt atacccaaag ttgaaggcac tactatagca gatcaaatat tacgatatgg 18780
cctgcccacc tgatgttata cccaaagttg aaggcactac tatagcagat caaatattac 18840
gatatggtag cttaggggtg ttttttggag gtttaggcat gcactactat agcagatcaa 18900
atattacgat atggtagctt aggggtgttt tttggaggtt taggcattgg tacagggtcg 18960
ggtacaggtg gcaggactgg gtagcttagg ggtgtttttt ggaggtttag gcattggtac 19020
agggtcgggt acaggtggca ggactggata tgtgcccctt ggtagtaccc caccgtctga 19080
ttggtacagg gtcgggtaca ggtggcagga ctggatatgt gccccttggt agtaccccac 19140
cgtctgaggc tataccttta cagcccatac gtcccccagt atatgtgccc cttggtagta 19200
ccccaccgtc tgaggctata cctttacagc ccatacgtcc cccagttacc gttgatactg 19260
tggggccttt ggattcttct gaggctatac ctttacagcc catacgtccc ccagttaccg 19320
ttgatactgt ggggcctttg gattcttcta ttgtatcttt aatagaggaa tctagtttta 19380
ttaccgttga tactgtgggg cctttggatt cttctattgt atctttaata gaggaatcta 19440
gttttataga cgccggtgca ccagccccat caattcccac ctattgtatc tttaatagag 19500
gaatctagtt ttatagacgc cggtgcacca gccccatcaa ttcccactcc atctggtttt 19560
gatattacca cctctgcaga gttttataga cgccggtgca ccagccccat caattcccac 19620
tccatctggt tttgatatta ccacctctgc agatactaca cctgcaatac ttaatgtttc 19680
tagacgccgg tgcaccagcc ccatcaattc ccactccatc tggttttgat attaccacct 19740
ctgcagatac tacacctgca atacttaatg tttcctctat ctccatctgg ttttgatatt 19800
accacctctg cagatactac acctgcaata cttaatgttt cctctattgg agaatcatct 19860
atacaaactg tttctacaca ctgcagatac tacacctgca atacttaatg tttcctctat 19920
tggagaatca tctatacaaa ctgtttctac acatttaaat ccctccttta ctgagccatc 19980
ctacacctgc aatacttaat gtttcctcta ttggagaatc atctatacaa actgtttcta 20040
cacatttaaa tccctccttt actgagccat ccgtactccg catttaaatc cctcctttac 20100
tgagccatcc gtactccgcc ctcctgcacc tgcagaggcc tctggacatt taatattttc 20160
ctctcctact gttagcacac ctccgccctc ctgcacctgc agaggcctct ggacatttaa 20220
tattttcctc tcctactgtt agcacacata gttatgaaaa cataccaatg gatacctttg 20280
tttaatattt tcctctccta ctgttagcac acatagttat gaaaacatac caatggatac 20340
ctttgttatt tctactgaca gtggcaatgt cacgtctagc agcacacata gttatgaaaa 20400
cataccaatg gatacctttg ttatttctac tgacagtggc aatgtcacgt ctagcacacc 20460
cattccaggg tctcgccctg agttatgaaa acataccaat ggataccttt gttatttcta 20520
ctgacagtgg caatgtcacg tctagcacac ccattccagg gtctcgccct gtggcacgcc 20580
ttatttctac tgacagtggc aatgtcacgt ctagcacacc cattccaggg tctcgccctg 20640
tggcacgcct tggtttatac agtcgcaaca cccaacaagt agcacaccca ttccagggtc 20700
tcgccctgtg gcacgccttg gtttatacag tcgcaacacc caacaagtta aggttgttga 20760
ccctgctttt ttaacgtggc acgccttggt ttatacagtc gcaacaccca acaagttaag 20820
gttgttgacc ctgctttttt aacatctcct catagacttg taacatatga taatccgcct 20880
tggtttatac agtcgcaaca cccaacaagt taaggttgtt gaccctgctt ttttaacatc 20940
tcctcataga cttgtaacat atgataatcc agcatcccaa caagttaagg ttgttgaccc 21000
tgctttttta acatctcctc atagacttgt aacatatgat aatccagcat ttgaaggctt 21060
taaccctgag gacacgttaa ggttgttgac cctgcttttt taacatctcc tcatagactt 21120
gtaacatatg ataatccagc atttgaaggc tttaaccctg aggacacatt gcagtctcat 21180
agacttgtaa catatgataa tccagcattt gaaggcttta accctgagga cacattgcag 21240
tttcaacata gtgacatatc gcctgctcct gatccttgaa ggctttaacc ctgaggacac 21300
attgcagttt caacatagtg acatatcgcc tgctcctgat cctgattttc tagatattgt 21360
tgcattacac agaccttcaa catagtgaca tatcgcctgc tcctgatcct gattttctag 21420
atattgttgc attacacaga cctgcattaa cctctcgcag gggtactgta cgttactgat 21480
tttctagata ttgttgcatt acacagacct gcattaacct ctcgcagggg tactgtacgt 21540
tatagtaggg ttgggcaaaa ggctacactt cgtaccctgc attaacctct cgcaggggta 21600
ctgtacgtta tagtagggtt gggcaaaagg ctacacttcg tactcgcagt ggaaagcaaa 21660
taggggctaa agtacagtag ggttgggcaa aaggctacac ttcgtactcg cagtggaaag 21720
caaatagggg ctaaagtaca ttactaccaa gacttaagtc ccatacagcc tgtccctcgc 21780
agtggaaagc aaataggggc taaagtacat tactaccaag acttaagtcc catacagcct 21840
gtccaggaac aggtacaaca gcagcaacaa tttgacatta ctaccaagac ttaagtccca 21900
tacagcctgt ccaggaacag gtacaacagc agcaacaatt tgaattacaa tctttaaata 21960
cttctgtttc tcccttccag gaacaggtac aacagcagca acaatttgaa ttacaatctt 22020
taaatacttc tgtttctccc tatagtatta atgatggact ttatgatatt tcgtaaatta 22080
caatctttaa atacttctgt ttctccctat agtattaatg atggacttta tgatatttcg 22140
tatgacgcgt atgatactat acatgatttt cagagatagt attaatgatg gactttatga 22200
tatttcgtat gacgcgtatg atactataca tgattttcag agtcctctgc actcacatac 22260
gtcctttgcc accacctgac gcgtatgata ctatacatga ttttcagagt cctctgcact 22320
cacatacgtc ctttgccacc acacgtacca gtaatgtgtc cataccatta aatacgtcct 22380
ctgcactcac atacgtcctt tgccaccaca cgtaccagta atgtgtccat accattaaat 22440
actggatttg acactcctct tgtgtcattg gaacccacgt accagtaatg tgtccatacc 22500
attaaatact ggatttgaca ctcctcttgt gtcattggaa cctggtccag acattgcatc 22560
ttctgtaaca tctatctgga tttgacactc ctcttgtgtc attggaacct ggtccagaca 22620
ttgcatcttc tgtaacatct atgtctagtc catttattcc tatatctcca ctaaccctgg 22680
tccagacatt gcatcttctg taacatctat gtctagtcca tttattccta tatctccact 22740
aactcctttt aataccataa ttgtggatgg tgctgtgtct agtccattta ttcctatatc 22800
tccactaact ccttttaata ccataattgt ggatggtgct gattttatgt tgcaccctag 22860
ctattttatt ttgcgctcct tttaatacca taattgtgga tggtgctgat tttatgttgc 22920
accctagcta ttttattttg cgtcgcagac gtaaacgttt tccatatttt tttgcatttt 22980
atgttgcacc ctagctattt tattttgcgt cgcagacgta aacgttttcc atattttttt 23040
gcagatgtcc gtgtggcggc ctagtgaggc cactgcctag ctattttatt ttgcgtcgca 23100
gacgtaaacg ttttccatat ttttttgcag atgtccgtgt ggcggcctag tgaggccact 23160
gtgtacctgc ctcgcagacg taaacgtttt ccatattttt ttgcagatgt ccgtgtggcg 23220
gcctagtgag gccactgtgt acctgcctcc tgtgcctgtg tctaaggttg tcgtaaacgt 23280
tttccatatt tttttgcaga tgtccgtgtg gcggcctagt gaggccactg tgtacctgcc 23340
tcctgtgcct gtgtctaagg ttgtttttgc agatgtccgt gtggcggcct agtgaggcca 23400
ctgtgtacct gcctcctgtg cctgtgtcta aggttgtaag cactgatgaa tatgtgtcac 23460
gcacagatgt ccgtgtggcg gcctagtgag gccactgtgt acctgcctcc tgtgcctgtg 23520
tctaaggttg taagcactga tgaatatgtg tcacgcacaa gcatccgtgt ggcggcctag 23580
tgaggccact gtgtacctgc ctcctgtgcc tgtgtctaag gttgtaagca ctgatgaata 23640
tgtgtcacgc acaagcattt attatgtggc ggcctagtga ggccactgtg tacctgcctc 23700
ctgtgcctgt gtctaaggtt gtaagcactg atgaatatgt gtcacgcaca agcataggcc 23760
actgtgtacc tgcctcctgt gcctgtgtct aaggttgtaa gcactgatga atatgtgtca 23820
cgcacaagca tttattattc gtatggcagt tcgtgtacct gcctcctgtg cctgtgtcta 23880
aggttgtaag cactgatgaa tatgtgtcac gcacaagcat ttattattcg tatggcagtt 23940
ccagactttt gggcctcctg tgcctgtgtc taaggttgta agcactgatg aatatgtgtc 24000
acgcacaagc atttattatt cgtatggcag ttccagactt ttggctgttg gcgtaagcac 24060
tgatgaatat gtgtcacgca caagcattta ttattcgtat ggcagttcca gacttttggc 24120
tgttggcaat ccatattttt ccatcaaaag tcatttatta ttcgtatggc agttccagac 24180
ttttggctgt tggcaatcca tatttttcca tcaaaagtcc caataacaat aaaaaagtat 24240
tagttcccaa gggctgttgg caatccatat ttttccatca aaagtcccaa taacaataaa 24300
aaagtattag ttcccaaggt atcaggctta cagtataggg tctttagggt gccccaataa 24360
caataaaaaa gtattagttc ccaaggtatc aggcttacag tatagggtct ttagggtgcg 24420
tttacctgat cccaataaat ttggttttcc tgtattagtt cccaaggtat caggcttaca 24480
gtatagggtc tttagggtgc gtttacctga tcccaataaa tttggttttc ctgatacatc 24540
tttttataac ccggtatcag gcttacagta tagggtcttt agggtgcgtt tacctgatcc 24600
caataaattt ggttttcctg atacatcttt ttataaccct gatacacaac gtgcgtttac 24660
ctgatcccaa taaatttggt tttcctgata catcttttta taaccctgat acacaacgtt 24720
tggtctgggc atgtgtaggc cttgaaatag gtgatcccaa taaatttggt tttcctgata 24780
catcttttta taaccctgat acacaacgtt tggtctgggc atgtgtaggc cttgaaatag 24840
gtaggataca tctttttata accctgatac acaacgtttg gtctgggcat gtgtaggcct 24900
tgaaataggt aggggacagc cattgggtgt tggcgtaagt ggtccttttt ataaccctga 24960
tacacaacgt ttggtctggg catgtgtagg ccttgaaata ggtaggggac agccattggg 25020
tgttggcgta agtggtcatc cttggtctgg gcatgtgtag gccttgaaat aggtagggga 25080
cagccattgg gtgttggcgt aagtggtcat ccttatttaa ataaatttga tgacactgaa 25140
accggcatgt gtaggccttg aaataggtag gggacagcca ttgggtgttg gcgtaagtgg 25200
tcatccttat ttaaataaat ttgatgacac tgaaaccagt aacgaaatag gtaggggaca 25260
gccattgggt gttggcgtaa gtggtcatcc ttatttaaat aaatttgatg acactgaaac 25320
cagtaacaga tataggggac agccattggg tgttggcgta agtggtcatc cttatttaaa 25380
taaatttgat gacactgaaa ccagtaacag atatcccgca cagccagggt ctgcatcctt 25440
atttaaataa atttgatgac actgaaacca gtaacagata tcccgcacag ccagggtctg 25500
ataacaggga cgtattatct atggattata aacttgatga cactgaaacc agtaacagat 25560
atcccgcaca gccagggtct gataacaggg acgtattatc tatggattat aaacaaacac 25620
aattatgttt aatccagtaa cagatatccc gcacagccag ggtctgataa cagggacgta 25680
ttatctatgg attataaaca aacacaatta tgtttaattg gctgtaaacc tccgataaca 25740
gggacgtatt atctatggat tataaacaaa cacaattatg tttaattggc tgtaaacctc 25800
ccactggtga gcattggggt aaaggtgttg cctgggacgt attatctatg gattataaac 25860
aaacacaatt atgtttaatt ggctgtaaac ctcccactgg tgagcattgg ggtaaaggtg 25920
ttgcctgtac aaacacaatt atgtttaatt ggctgtaaac ctcccactgg tgagcattgg 25980
ggtaaaggtg ttgcctgtaa caatacgtaa gctgctactg attgtcctcc ccactggtga 26040
gcattggggt aaaggtgttg cctgtaacaa tacgtaagct gctactgatt gtcctccatt 26100
ggaacttttt aattctatta ttgaggatgt gtaacaatac gtaagctgct actgattgtc 26160
ctccattgga actttttaat tctattattg aggatggtga catggtagat acagggtttg 26220
gcgtaatggc cattggaact ttttaattct attattgagg atggtgacat ggtagataca 26280
gggtttggcg taatggactt tggtacattg caggctaata aaagtgatgc tattattgag 26340
gatggtgaca tggtagatac agggtttggc gtaatggact ttggtacatt gcaggctaat 26400
aaaagtgatg tgcctattgg gtgacatggt agatacaggg tttggcgtaa tggactttgg 26460
tacattgcag gctaataaaa gtgatgtgcc tattgatatt tgtaacagta ccgtaaaata 26520
cagggtttgg cgtaatggac tttggtacat tgcaggctaa taaaagtgat gtgcctattg 26580
atatttgtaa cagtaccgta aaatatccag atggactttg gtacattgca ggctaataaa 26640
agtgatgtgc ctattgatat ttgtaacagt accgtaaaat atccagatta tttaaaaatg 26700
gccagtgaac ctgtgcctat tgatatttgt aacagtaccg taaaatatcc agattattta 26760
aaaatggcca gtgaacctta tggggatagt ttgttctttt ttcttagacg tgatccagat 26820
tatttaaaaa tggccagtga accttatggg gatagtttgt tcttttttct tagacgtgag 26880
cagatgtttg ttagacactt ttttaatagg gctatgggga tagtttgttc ttttttctta 26940
gacgtgagca gatgtttgtt agacactttt ttaatagggc tggaaaactt ggcgaggctg 27000
tcccggatga ccgagcagat gtttgttaga cactttttta atagggctgg aaaacttggc 27060
gaggctgtcc cggatgacct ttatattaaa gggtccggta atactgcagt tagctggaaa 27120
acttggcgag gctgtcccgg atgaccttta tattaaaggg tccggtaata ctgcagttat 27180
ccaaagtagt gcattttttc caactcctag tgcctttata ttaaagggtc cggtaatact 27240
gcagttatcc aaagtagtgc attttttcca actcctagtg gctctatagt tacctcagaa 27300
tcacaattat ttatccaaag tagtgcattt tttccaactc ctagtggctc tatagttacc 27360
tcagaatcac aattatttaa taagccttat tggctacagc gtgcacaagg tcggctctat 27420
agttacctca gaatcacaat tatttaataa gccttattgg ctacagcgtg cacaaggtca 27480
taacaatggc atttgctggg gcaatcagtt ataataagcc ttattggcta cagcgtgcac 27540
aaggtcataa caatggcatt tgctggggca atcagttatt tgttaccgtg gttgatacca 27600
ctcgtagcac tacataacaa tggcatttgc tggggcaatc agttatttgt taccgtggtt 27660
gataccactc gtagcactaa tatgacattc gtaactgaag taactaagga agtggcattt 27720
gctggggcaa tcagttattt gttaccgtgg ttgataccac tcgtagcact aatatgacat 27780
tcgtaactga agtaactaag gaaggtacat attttgttac cgtggttgat accactcgta 27840
gcactaatat gacattcgta actgaagtaa ctaaggaagg tacatataaa aatgataatt 27900
ttaaggaata tgaatatgac attcgtaact gaagtaacta aggaaggtac atataaaaat 27960
gataatttta aggaatatgt acgtcatgtt gaagaatatg acttacagtt tgggtacata 28020
taaaaatgat aattttaagg aatatgtacg tcatgttgaa gaatatgact tacagtttgt 28080
ttttcagctt tgcaaaatta cactaactgc aggtacgtca tgttgaagaa tatgacttac 28140
agtttgtttt tcagctttgc aaaattacac taactgcaga gataatgaca tatatacata 28200
ctatggattc cagtttttca gctttgcaaa attacactaa ctgcagagat aatgacatat 28260
atacatacta tggattccaa tattttggag gactggcaat ttggtttaac acgagataat 28320
gacatatata catactatgg attccaatat tttggaggac tggcaatttg gtttaacacc 28380
tcctccgtct gccagtttac aggacacata taccaatatt ttggaggact ggcaatttgg 28440
tttaacacct cctccgtctg ccagtttaca ggacacatat agatttgtta cctcccaggc 28500
tattacttgc cacctcctcc gtctgccagt ttacaggaca catatagatt tgttacctcc 28560
caggctatta cttgccaaaa aacagcaccc cctaaagaaa aggaagatcc atagatttgt 28620
tacctcccag gctattactt gccaaaaaac agcaccccct aaagaaaagg aagatccatt 28680
aaataaatat actttttggg aggttaactt aaaaacagca ccccctaaag aaaaggaaga 28740
tccattaaat aaatatactt tttgggaggt taacttaaag gaaaagtttt ctgcagatct 28800
agatcagttt cccccctaaa gaaaaggaag atccattaaa taaatatact ttttgggagg 28860
ttaacttaaa ggaaaagttt tctgcagatc tagatcagtt tcctttttaa ataaatatac 28920
tttttgggag gttaacttaa aggaaaagtt ttctgcagat ctagatcagt ttcctttggg 28980
acgaaagttt ttattacaat caggccatat actttttggg aggttaactt aaaggaaaag 29040
ttttctgcag atctagatca gtttcctttg ggacgaaagt ttttattaca atcaggcctt 29100
aaagcaagga aaagttttct gcagatctag atcagtttcc tttgggacga aagtttttat 29160
tacaatcagg ccttaaagca aagcccagac taaaacgttc ggcccccctt tgggacgaaa 29220
gtttttatta caatcaggcc ttaaagcaaa gcccagacta aaacgttcgg cccctactac 29280
ccgtgcacca tccaccaaac gcaaaacctt aaagcaaagc ccagactaaa acgttcggcc 29340
cctactaccc gtgcaccatc caccaaacgc aaaaaggtta aaaaataatt gttgtggtac 29400
ttacacgccc ctactacccg tgcaccatcc accaaacgca aaaaggttaa aaaataattg 29460
ttgtggtact tacactattt tattatacat gtttgtttgt tttatgaagg ttaaaaaata 29520
attgttgtgg tacttacact attttattat acatgtttgt ttgttttatg tatgtgttgt 29580
ctgtttgttt atgtttgtgt atatgtctat tttattatac atgtttgttt gttttatgta 29640
tgtgttgtct gtttgtttat gtttgtgtat atgttgtatg tgttatgtgt catgtttgtg 29700
tacatgtgtg ttgtctgttt gtttatgttt gtgtatatgt tgtatgtgtt atgtgtcatg 29760
tttgtgtaca tgttctatgt ccttgtcagt ttcctgtttc tgtatagttg tatgtgttat 29820
gtgtcatgtt tgtgtacatg ttctatgtcc ttgtcagttt cctgtttctg tatatatgta 29880
ataaactatt gtgtgtattg taaactctat gtccttgtca gtttcctgtt tctgtatata 29940
tgtaataaac tattgtgtgt attgtaaact atttgtattg tttgggtgta tctatgagta 30000
aggtgcatat gtaataaact attgtgtgta ttgtaaacta tttgtattgt ttgggtgtat 30060
ctatgagtaa ggtgctgtcc ctaaattgcc ctaccctgcc ctgcctattt gtattgtttg 30120
ggtgtatcta tgagtaaggt gctgtcccta aattgcccta ccctgccctg cctattcgta 30180
atacctatgt aatagtattt gtatgagctg tccctaaatt gccctaccct gccctgccta 30240
ttcgtaatac ctatgtaata gtatttgtat gatatgtatt ttatagtttt taacagtact 30300
gcctccttcg taatacctat gtaatagtat ttgtatgata tgtattttat agtttttaac 30360
agtactgcct ccattttact ttacctccat tttgtgcatg taaccgccat tttactttac 30420
ctccattttg tgcatgtaac cgatttcggt tgctggcaca aacgtgtttt ttttaaacta 30480
caatttaaac aatacagtta atccttccga tttcggttgc tggcacaaac gtgttttttt 30540
taaactacaa tttaaacaat acagttaatc ctttcccttc ctgcactgct tttgcctata 30600
cttgcattaa actacaattt aaacaataca gttaatcctt tcccttcctg cactgctttt 30660
gcctatactt gcatatgtga ctcatatata ccgtaagtgc agttgctccc ttcctgcact 30720
gcttttgcct atacttgcat atgtgactca tatataccgt aagtgcagtt gcaaaatgtt 30780
taattatact catagtttaa accgtatatg tgactcatat ataccgtaag tgcagttgca 30840
aaatgtttaa ttatactcat agtttaaacc gtattatagg cacatatttt aacttacttt 30900
cacgtaactc atatataccg taagtgcagt tgcaaaatgt ttaattatac tcatagttta 30960
aaccgtatta taggcacata ttttaactta ctttcacgta ttaagcaaaa tgtttaatta 31020
tactcatagt ttaaaccgta ttataggcac atattttaac ttactttcac gtattaagtg 31080
cagttttggc ttgcacaata gtttggctta taggcacata ttttaactta ctttcacgta 31140
ttaagtgcag ttttggcttg cacaatagtt tgttcgtaca aactatgtct tgtaaaagtg 31200
actcagctta agtgcagttt tggcttgcac aatagtttgt tcgtacaaac tatgtcttgt 31260
aaaagtgact cactaacatt tattgccagg tgtggactaa ccgttgttcg tacaaactat 31320
gtcttgtaaa agtgactcac taacatttat tgccaggtgt ggactaaccg ttttgggtca 31380
cattgttcat gtttcaacat tttat 31405
<210> 6
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 6
caagcagaag acggcatacg agattgcatg acgtgactgg agttcagacg tgt 53
<210> 7
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 7
caagcagaag acggcatacg agattgctat cggtgactgg agttcagacg tgt 53
<210> 8
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 8
caagcagaag acggcatacg agatcacaag cagtgactgg agttcagacg tgt 53
<210> 9
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 9
caagcagaag acggcatacg agattcgctg atgtgactgg agttcagacg tgt 53
<210> 10
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 10
caagcagaag acggcatacg agatcgcttt gtgtgactgg agttcagacg tgt 53
<210> 11
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 11
caagcagaag acggcatacg agatccgatg aagtgactgg agttcagacg tgt 53
<210> 12
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 12
caagcagaag acggcatacg agattgacca cagtgactgg agttcagacg tgt 53
<210> 13
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 13
caagcagaag acggcatacg agatgaagcc atgtgactgg agttcagacg tgt 53
<210> 14
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 14
caagcagaag acggcatacg agatttctgg tggtgactgg agttcagacg tgt 53
<210> 15
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 15
caagcagaag acggcatacg agatccgaaa acgtgactgg agttcagacg tgt 53
<210> 16
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 16
caagcagaag acggcatacg agatcgaaaa gggtgactgg agttcagacg tgt 53
<210> 17
<211> 53
<212> DNA
<213> Artificial Sequence
<220>
<223> 引物
<400> 17
caagcagaag acggcatacg agataaccag ctgtgactgg agttcagacg tgt 53
<210> 18
<211> 60
<212> DNA
<213> Artificial Sequence
<220>
<223> 接头
<400> 18
ctacaataat tcatgtataa aactaagggc tagtataaaa gcagacattt tcgtaaccaa 60
Claims (3)
1.一种用于检测5种人***瘤病毒HPV分型和整合的探针组，其特征在于包含以下探针：SEQ ID NO.1、SEQ ID NO.2、SEQ ID NO.3、SEQ ID NO.4和SEQ ID NO.5；所述5种人***瘤病毒HPV为HPV16、18、33、52、58；所述探针均为叠瓦式设计，由首尾相连的多条探针组成。
2.一种用于检测人***瘤病毒HPV分型和整合的试剂盒，其特征在于包含权利要求1所述的探针组。
3.权利要求1所述的探针组用于非诊断非治疗目的人***瘤病毒HPV分型和整合检测。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011260473.7A CN112522439B (zh) | 2020-11-12 | 2020-11-12 | 一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011260473.7A CN112522439B (zh) | 2020-11-12 | 2020-11-12 | 一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN112522439A CN112522439A (zh) | 2021-03-19 |
| CN112522439B true CN112522439B (zh) | 2024-01-30 |
Family
ID=74982235
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011260473.7A Active CN112522439B (zh) | 2020-11-12 | 2020-11-12 | 一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112522439B (zh) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450885A (zh) * | 2014-10-29 | 2015-03-25 | 百世诺（北京）医疗科技有限公司 | 一种检测神经纤维瘤病相关基因突变的试剂盒 |
| CN107739761A (zh) * | 2016-08-12 | 2018-02-27 | 嘉兴允英医学检验有限公司 | 一种用于hpv分型及整合的高通量测序检测方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9809864B2 (en) * | 2013-03-01 | 2017-11-07 | The Johns Hopkins University | Dual sequence-capture method for quantifying trans renal HPV DNA in urine |
-
2020
- 2020-11-12 CN CN202011260473.7A patent/CN112522439B/zh active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450885A (zh) * | 2014-10-29 | 2015-03-25 | 百世诺（北京）医疗科技有限公司 | 一种检测神经纤维瘤病相关基因突变的试剂盒 |
| CN107739761A (zh) * | 2016-08-12 | 2018-02-27 | 嘉兴允英医学检验有限公司 | 一种用于hpv分型及整合的高通量测序检测方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN112522439A (zh) | 2021-03-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112195287B (zh) | 一种用于人***瘤病毒hpv分型和整合检测的探针组及其试剂盒 | |
| DK2644712T3 (en) | A method for diagnosing neoplasms | |
| CN109863251B (zh) | 对肺鳞状细胞癌亚型分型的方法 | |
| KR20180093902A (ko) | 태아와 임신 여성간에 상이하게 메틸화된 디엔에이 영역을 이용한 태아 염색체 이수성의 검출 | |
| CN109423515A (zh) | 一组用于肝癌检测的基因标志物及其应用 | |
| CN112266983B (zh) | 一种用于检测人***瘤病毒hpv16和hpv18的探针组及其试剂盒 | |
| CN116042678A (zh) | 一种用于检测神经梅毒的融合蛋白及其试剂盒 | |
| KR20220156899A (ko) | 결장직장 신생물의 스크리닝을 위한 방법 및 키트 | |
| CN112522439B (zh) | 一种用于检测5种人***瘤病毒hpv分型和整合的探针组及其试剂盒 | |
| KR102510442B1 (ko) | 극미량 중증열성혈소판감소증후군 바이러스, 아데노 바이러스 및 신종코로나 바이러스 검출용 프로브 세트, 이를 이용한 중증열성혈소판감소증후군 바이러스, 아데노 바이러스 및 신종코로나 바이러스의 전장 유전체 서열 분석 및 검출 방법 | |
| KR20200004977A (ko) | 암을 유발할 수 있는 유전자 변이를 검출하기 위한 프로브와 제조 및 검출 방법 | |
| CN106460047B (zh) | 用于鉴定癌前结肠直肠息肉和结肠直肠癌的方法及试剂盒 | |
| CN108676797B (zh) | 用于检测人***瘤病毒的成套试剂与方法 | |
| CN112195286B (zh) | 一种用于检测人***瘤病毒hpv26的探针及其试剂盒 | |
| CN114032298B (zh) | 用于检测遗传性胆红素代谢异常及肝内胆汁淤积症相关基因变异的探针组、试剂盒及其应用 | |
| KR102458473B1 (ko) | Snp를 검출 또는 증폭할 수 있는 제제를 포함하는 소의 등심단면적 판별용 조성물 및 이를 포함하는 키트 | |
| CN112195280B (zh) | 一种用于检测人***瘤病毒hpv39的探针及其试剂盒 | |
| KR102480128B1 (ko) | 면역력 강화 소 African Humped Cattle (AFH) 품종 특이적 단일염기다형성 및 그의 용도 | |
| KR102458466B1 (ko) | Snp를 검출 또는 증폭할 수 있는 제제를 포함하는 소의 등지방두께 판별용 조성물 및 이를 포함하는 키트 | |
| CN112301166A (zh) | 一种用于检测人***瘤病毒hpv51的探针及其试剂盒 | |
| CN112195281A (zh) | 一种用于检测人***瘤病毒hpv56的探针及其试剂盒 | |
| CN111172270B (zh) | 用于子***内病变分级的全血转录基因标志物及其应用 | |
| TW201827602A (zh) | 辨識轉移性腫瘤的原發位置之方法及系統 | |
| CN112301164A (zh) | 一种用于检测人***瘤病毒hpv66的探针及其试剂盒 | |
| CN108913761A (zh) | 一种用于筛查遗传性肝病的试剂盒 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |