CN112517093A - Fish saliva automatic sample separation detection disc and detection method thereof - Google Patents
Fish saliva automatic sample separation detection disc and detection method thereof Download PDFInfo
- Publication number
- CN112517093A CN112517093A CN202011287425.7A CN202011287425A CN112517093A CN 112517093 A CN112517093 A CN 112517093A CN 202011287425 A CN202011287425 A CN 202011287425A CN 112517093 A CN112517093 A CN 112517093A
- Authority
- CN
- China
- Prior art keywords
- saliva
- slide
- fish
- glass
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000003296 saliva Anatomy 0.000 title claims abstract description 59
- 238000001514 detection method Methods 0.000 title claims abstract description 51
- 238000000926 separation method Methods 0.000 title claims abstract description 21
- 239000011521 glass Substances 0.000 claims abstract description 48
- 239000000523 sample Substances 0.000 claims abstract description 46
- 239000000758 substrate Substances 0.000 claims abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 13
- 201000010099 disease Diseases 0.000 claims abstract description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 11
- 238000001917 fluorescence detection Methods 0.000 claims abstract description 3
- 238000011282 treatment Methods 0.000 claims description 8
- 238000001612 separation test Methods 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 108020004707 nucleic acids Proteins 0.000 abstract description 2
- 150000007523 nucleic acids Chemical class 0.000 abstract description 2
- 102000039446 nucleic acids Human genes 0.000 abstract description 2
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 238000005070 sampling Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 208000025157 Oral disease Diseases 0.000 description 2
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 210000000214 mouth Anatomy 0.000 description 2
- 208000030194 mouth disease Diseases 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 241000606749 Aggregatibacter actinomycetemcomitans Species 0.000 description 1
- 239000012103 Alexa Fluor 488 Substances 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 238000002738 Giemsa staining Methods 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 241000605862 Porphyromonas gingivalis Species 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 201000011529 cardiovascular cancer Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 238000011086 high cleaning Methods 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010340 saliva test Methods 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50855—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates using modular assemblies of strips or of individual wells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
The invention relates to a Fish saliva automatic sample separation detection disc and a detection method thereof, and solves the problems that in the prior art, a saliva detection mode is complex in process, insufficient in accuracy and difficult to detect quickly and simultaneously. The device is provided with a substrate, a central collecting area, a diversion capillary tube and a slide glass carrier to form a channel for holding saliva, separating samples and holding a slide glass, and the channel is of an integrated structure, wherein the slide glass is detachable. After the collected saliva is placed in the central collection area, the liquid and the capillary wall automatically flow into the diversion capillary to the glass slide on the glass slide carrier through the capillary phenomenon. After the glass slide is disassembled, the content of the microorganisms is judged according to the Fish technical principle and the species of the detected microorganisms, and the disease process is indicated. Multiple slide stages can detect multiple microorganisms simultaneously without interfering with each other. The saliva is automatically subpackaged quickly and uniformly, and is fused with a Fish fluorescence detection technology, and a specific fluorescent probe is combined with the microbial nucleic acid to quantitatively detect the microbes. Simplify saliva detection, improve saliva detection's accuracy.
Description
Technical Field
The invention relates to the field of medical technology diagnosis, in particular to a Fish saliva self-sampling detection disc and a detection method thereof.
Background
Saliva is an important body fluid secreted by the salivary glands of the oral cavity, is rich in biomolecules, and can be changed along with the physical health condition, wherein related biomarkers can be used for monitoring oral cavity and systemic diseases. Saliva detection has the advantages of being noninvasive, convenient, rapid, easy to accept by patients and the like, so that more and more attention is paid to the saliva detection. At present, the relation between various diseases such as new coronary pneumonia, oral diseases, cardiovascular diseases, cancers and the like and corresponding pathogenic bacteria and secretions thereof is clearly researched, and researches prove that the types and the proportions of flora have good correlation with the diseases. Therefore, the sensitivity and the accuracy of disease detection can be greatly improved by combining the specific microorganisms with some clinical indexes, and the detection of the abundance or the proportion change can also be used for judging the risk of diseases, helping patients to take treatment measures as soon as possible and avoiding unnecessary pain and burden caused by delay.
In addition, in recent years, the Fish technology has gradually emerged, and has the advantages of 1, economy and safety of a fluorescent reagent and a probe as a non-radioactive detection system; 2. the probe is stable and can be used within two years after being labeled once; 3. the experimental period is short, the result can be obtained quickly, the specificity is good, the positioning is accurate, and the sensitivity is high; 4. multicolor FISH can detect multiple sequences simultaneously, etc. by displaying different colors in the same nucleus.
In the prior art, the Fish saliva automatic sample separation detection disc is designed, and the saliva is automatically separated and placed on eight glass slides, so that each glass slide can be applied to detection of different microorganisms, the required saliva extraction times are greatly reduced, and the detection efficiency is improved. After the detachable glass slide is processed and the pre-configured fluorescent probe is placed in the detachable glass slide, the detachable glass slide is placed back to the device for fluorescent detection at the same time, so that various disease detection indexes can be obtained at one time, and diseases can be diagnosed efficiently and comprehensively.
Disclosure of Invention
In view of the above analysis, the present invention aims to provide a Fish saliva automatic sample separation detection tray, which is used for solving the problems of complicated procedures, insufficient accuracy, difficulty in rapid and simultaneous detection in the saliva detection mode in the prior art.
The purpose of the invention is mainly realized by the following technical scheme:
in the technical scheme of the invention, the Fish saliva automatic sample separation detection disc device comprises: the device comprises a substrate, a central collecting area, a diversion capillary tube and a slide carrying platform.
In the technical scheme of the invention, the substrate, the central searching and collecting area, the flow guide capillary tube and the slide carrying platform are of an integrated structure and cannot be disassembled.
Furthermore, the central searching and collecting area, the flow guide capillary tube and the slide glass carrying platform are sequentially arranged and embedded above the machine body to form a channel for placing saliva, separating samples and placing slide glass, and the channel is of an integrated structure.
Further, the slide stage may be for a slide to be placed.
Further, the slide is freely detachable.
A use method of a Fish saliva automatic sample separation detection disc is characterized in that after saliva is automatically separated and guided into a glass slide by the Fish saliva automatic sample separation detection disc, according to the Fish technical step principle, the glass slide is subjected to a series of treatments and then inserted back to a glass slide carrier for one-time fluorescence detection, so that the content of microorganisms is judged, and a disease process is indicated: comprises the following steps;
s1, collecting a proper amount of saliva and filling the saliva into a central collection area;
s2, automatically flowing saliva in the central collection area into a glass slide placed above the glass slide carrier along a diversion capillary tube through a capillary phenomenon;
s3, disassembling the glass slide, performing characteristic treatment on different glass slides according to the Fish technical step principle and the detected microorganism species, and putting specific probes;
and S4, inserting the processed slide back to the slide carrier, placing the slide carrier under a fluorescence microscope for scanning, and analyzing the result.
On one hand, the invention can use the Fish saliva automatic sample separation detection disc matched with probes and reagents to detect a plurality of common oral diseases, and can also use the Fish saliva automatic sample separation detection disc alone to select other microorganism specific probes and reagents to detect.
The matched probe and reagent of the invention and the application method are as follows:
Slide groove No. 2: detection of Streptococcus mutans, Gene Probe MUT590, 5'-ACTCCAGAC TTTCCTGAC-3'.
Slide groove No. 3: the gene probe 5'-CTGGAGAGACTAAGCCCTCC-3' modified by LNA, and the 5' end Cy5 marker (Abs/Em 550-592/633 nm, red) are used for detecting helicobacter pylori.
Slide No. 5 slide trough: detecting Porphyromonas gingivalis, wherein a gene probe 5 '-3': CACTGAACT CAAGCCCGGCAGTTTCAA and labeled with ALEXA Fluor 488.
No. 6 slide glass slide groove, detecting Actinobacillus actinomycetemcomitans, gene probe, sequence 5'-CACCAGGGCTAAACCCAAT-3', and labeling with FITC developing blue-green.
Slide slots No. 7 and No. 8 are spare slide slots or are used for other microbiological tests.
The matched reagent comprises: fluorescent medium oil, ethanol, paraformaldehyde, sodium dodecyl sulfate, Tris buffer solution, formalin, formamide/2 XSSC (sodium dodecyl sulfate)/denaturing solution, Giemsa staining agent, PI/anti-fiade staining agent, DAPI/anti-fiade staining agent and a universal probe.
The technical scheme of the invention can at least realize one of the following effects:
1. the Fish saliva automatic sample separation detection disc simplifies operation on the basis of the original saliva detection process, realizes simultaneous detection of multiple microorganisms in the same sample, and greatly improves saliva detection efficiency.
2. The symmetrical structure of the device enables the samples to be uniformly subpackaged, and the design of mutual separation of the device areas improves the operability of the experiment, provides sufficient variable experiment conditions, fully ensures the specificity of Fish technology detection conditions, and improves the accuracy of saliva detection.
3. The device has the advantages of flexible and simple design structure, easy disassembly and cleaning and high cleaning efficiency.
4. The device disclosed by the invention is combined with a fluorescein in-situ hybridization technology (Fish), and a mode of combining a specific fluorescent probe and microbial nucleic acid is adopted to realize rapid quantitative detection of microbes.
Additional features and advantages of the invention will be set forth in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. The objectives and other advantages of the invention will be realized and attained by the structure particularly pointed out in the written description and drawings.
Drawings
The drawings are only for purposes of illustrating particular embodiments and are not to be construed as limiting the invention, wherein like reference numerals are used to designate like parts throughout.
FIG. 1 is a schematic top view of the present invention;
FIG. 2 is an isometric view of the present invention;
FIG. 3 is a right side view of the present invention;
FIG. 4 is a schematic view of a slide assembly of the present invention.
Reference numerals:
1-a substrate; 2-a central collection region; 3-diversion capillary; 4-slide stage.
Detailed Description
The preferred embodiments of the present invention will now be described in detail with reference to the accompanying drawings, which form a part hereof, and which together with the embodiments of the invention serve to explain the principles of the invention and not to limit its scope.
In the description of the embodiments of the present invention, it should be noted that, unless otherwise explicitly stated or limited, the term "connected" should be interpreted broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection, which may be a mechanical connection, an electrical connection, which may be a direct connection, or an indirect connection via an intermediate medium. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "top," "bottom," "above … …," "below," and "on … …" as used throughout the description are relative positions with respect to components of the device, such as the relative positions of the top and bottom substrates inside the device. It will be appreciated that the devices are multifunctional, regardless of their orientation in space.
As shown in fig. 1 to 4, an embodiment of the present invention provides a Fish saliva automatic sample separation detection tray, including: a flow guide capillary tube (3) and a glass slide carrying platform (4) are arranged in the central collecting area (2) of the substrate (1); the central searching and collecting area (2), the flow guide capillary tube (3) and the slide carrying platform (4) are embedded above the substrate (1); the central searching and collecting area (2), the flow guide capillary tube (3) and the slide glass carrying platform (4) are sequentially connected and arranged to form a channel for placing saliva, automatically separating samples and placing slide glass, and the channel is of an integrated structure; the flow guide capillary tube (3) in the central collection area (2) of the substrate (1) and the slide carrying platform (4) are not detachable from the substrate (1); the slide glass carrier (4) can be used for placing the slide glass, and the slide glass can be freely detached. Wherein, the central collecting area (2) is a depression in the substrate (1) and is used for containing a saliva sample to be measured; the flow guide capillary tube (3) is connected with the central collecting area (2), and a part of saliva sample to be measured is guided to the slide glass carrying platform (4) by utilizing the capillary phenomenon; the slide glass carrier (4) is connected with the diversion capillary (3), a part of saliva samples are collected and then detached, different glass slides are processed differently according to the Fish technical step principle and the microbial species detection, then the slide glass carrier (4) is inserted back and placed under a fluorescence microscope for scanning, and the result is analyzed.
The invention relates to a Fish technology and an automatic sample separation technology, which are difficult to perform multiple examinations simultaneously due to the difficulty in sampling saliva and the small sampling amount in the traditional saliva detection, and have high time cost. According to the device, one saliva sample to be detected is automatically divided into eight parts on average, so that the novel saliva detection box collects the sample through the central collection area (2), the flow guide capillary tube (3) uniformly disperses the sample, and the sample is continuously drained to the slide glass carrying table (4) for subsequent operation.
After a sample to be detected is dripped in the central collecting area, liquid is in contact with the wall of the capillary tube to generate a capillary phenomenon, so that a part of liquid moves to the test paper detecting area and is absorbed by dry test paper, the sample is continuously and uniformly dispersed to each slide carrier, and the slide is disassembled for characteristic treatment and then inserted back to the slide carrier for scanning observation and result analysis.
The use method of the embodiment of the invention comprises the following steps:
step S1: collecting a proper amount of saliva and filling the saliva into a central collecting area (2);
step S2: saliva in the central collection area (2) automatically flows into a glass slide arranged above the glass slide carrying platform (4) along the diversion capillary tube (3) through capillary phenomenon;
step S3: disassembling the glass slide, performing characteristic treatment on different glass slides according to the Fish technical step principle and the detected microorganism species, and putting specific probes into the different glass slides;
step S4: the processed slide is inserted back into the slide stage (4) and placed under a fluorescence microscope for scanning and the results are analyzed.
Compared with the existing detection mode, the Fish saliva automatic sample separation detection disc provided by the embodiment of the invention has at least one of the following beneficial effects:
1. the traditional saliva test needs a large amount of saliva samples, and the testee may feel discomfort such as regurgitation during sampling. The invention can uniformly disperse the saliva sample into eight parts, reduce the times of saliva extraction and reduce the pain of the examined person.
2. One sample can be used for simultaneously detecting eight diseases, so that the detection efficiency is improved, and the time cost is saved.
3. By applying the Fish technology, the detection accuracy is improved, and the early diagnosis of the disease condition is facilitated, so that the early discovery, early diagnosis and early treatment are realized.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention.
Claims (5)
1. A Fish saliva automatic sample separation detection disc, comprising: a flow guide capillary tube (3) and a glass slide carrying platform (4) are arranged in the central collecting area (2) of the substrate (1); the central collection area (2), the flow guide capillary (3) and the slide carrying platform (4) are embedded above the substrate (1).
2. The Fish saliva automatic sample separation detection disc of claim 1, wherein the central collection area (2), the flow guide capillary (3) and the slide glass carrier (4) are sequentially connected and arranged to form a saliva placement, automatic sample separation and slide glass placement channel, and the structure is an integral structure.
3. A Fish saliva automatic sample separation detection disc according to claim 2, characterized in that the flow guide capillary (3) in the central collection area (2) of the substrate (1) and the slide carrier (4) of the substrate (1) are both non-detachable.
4. A Fish saliva automatic sample separation test tray according to claim 3, wherein the slide carrier (4) is adapted for holding a slide, and the slide is freely removable.
5. A use method of a Fish saliva automatic sample separation detection disc, which is characterized in that after saliva is automatically separated and guided into a glass slide by the Fish saliva automatic sample separation detection disc of claims 1-4, the glass slide is subjected to a series of treatments and then inserted back into a glass slide carrier (4) for disposable fluorescence detection according to the principle of Fish technical steps, so as to judge the content of microorganisms and indicate the disease process, and the method comprises the following steps:
step S1: collecting a proper amount of saliva and filling the saliva into a central collecting area (2);
step S2: saliva in the central collection area (2) automatically flows into a glass slide arranged above the glass slide carrying platform (4) along the diversion capillary tube (3) through capillary phenomenon;
step S3: disassembling the glass slide, performing characteristic treatment on different glass slides according to the Fish technical step principle and the detected microorganism species, and putting specific probes into the different glass slides;
step S4: the processed slide is inserted back into the slide stage (4) and placed under a fluorescence microscope for scanning and the results are analyzed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011287425.7A CN112517093A (en) | 2020-11-17 | 2020-11-17 | Fish saliva automatic sample separation detection disc and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011287425.7A CN112517093A (en) | 2020-11-17 | 2020-11-17 | Fish saliva automatic sample separation detection disc and detection method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112517093A true CN112517093A (en) | 2021-03-19 |
Family
ID=74980799
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011287425.7A Pending CN112517093A (en) | 2020-11-17 | 2020-11-17 | Fish saliva automatic sample separation detection disc and detection method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112517093A (en) |
Citations (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ219926A (en) * | 1986-06-26 | 1990-04-26 | Becton Dickinson Co | Apparatus and method for determining glucose in body fluids |
| CN1158657A (en) * | 1994-09-22 | 1997-09-03 | 伯纳德·查菲林恩 | Single-use device for detecting or analyzing a body fluid |
| CN1296525A (en) * | 1998-02-20 | 2001-05-23 | 内诺金有限公司 | Active device and method for molecular biological analysis and diagnostics |
| US20060257992A1 (en) * | 2004-02-27 | 2006-11-16 | Mcdevitt John T | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
| CN101036790A (en) * | 2006-03-17 | 2007-09-19 | 四川大学 | Protein genetic vaccines on the surface of mutans streptococci and its preparing method |
| AU2007329748A1 (en) * | 2006-10-25 | 2008-06-12 | Ikonisys, Inc. | Automated detection of cancer and high grade hyperplasias |
| CN101495869A (en) * | 2005-11-30 | 2009-07-29 | 麻省理工学院 | Pathogen detection biosensor |
| CN101583624A (en) * | 2006-09-29 | 2009-11-18 | 昂考梅德药品有限公司 | Compositions and methods for diagnosing and treating cancer |
| CN101661034A (en) * | 2008-08-28 | 2010-03-03 | 上海领潮生物科技有限公司 | Method for synchronously performing parallel detection of various biomarkers and chip test paper |
| CN104965096A (en) * | 2015-06-04 | 2015-10-07 | 南京普朗医疗设备有限公司 | Multi-sample immunity-detecting reaction disc capable of automatically separating serum |
| CN105518464A (en) * | 2013-07-05 | 2016-04-20 | 华盛顿大学商业中心 | Methods, compositions and systems for microfluidic assays |
| US9700888B1 (en) * | 2016-04-13 | 2017-07-11 | Ipmd Inc. | Point of care medical device for detecting infectious diseases |
| CN107805597A (en) * | 2017-09-29 | 2018-03-16 | 深圳国际旅行卫生保健中心 | Gene detection system and detection method based on micro-fluidic chip |
| CN107884561A (en) * | 2012-04-04 | 2018-04-06 | 辛辛那提大学 | sweat simulation, collection and sensing system |
| CN108070641A (en) * | 2018-01-24 | 2018-05-25 | 北京恩泽康泰生物科技有限公司 | It is used to detect the primer and probe of AR-V7 and AR in vesica based on qPCR or digital pcr technology |
| KR101905084B1 (en) * | 2017-07-13 | 2018-10-08 | 농업회사법인 주식회사 스피루리나팜스 | Light cultivating apparatus for culturing microalgae of self-circulating type |
| US20190078982A1 (en) * | 2017-09-14 | 2019-03-14 | Roy G. Whiteside, JR. | Biological fluid testing device |
| CN111108394A (en) * | 2017-12-12 | 2020-05-05 | 恩普乐股份有限公司 | Rack for placing analysis plate and analysis kit |
| CN111774103A (en) * | 2020-06-01 | 2020-10-16 | 东南大学 | Multi-core spiral inertia separation micro-fluidic device for high-throughput plasma separation |
-
2020
- 2020-11-17 CN CN202011287425.7A patent/CN112517093A/en active Pending
Patent Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ219926A (en) * | 1986-06-26 | 1990-04-26 | Becton Dickinson Co | Apparatus and method for determining glucose in body fluids |
| CN1158657A (en) * | 1994-09-22 | 1997-09-03 | 伯纳德·查菲林恩 | Single-use device for detecting or analyzing a body fluid |
| CN1296525A (en) * | 1998-02-20 | 2001-05-23 | 内诺金有限公司 | Active device and method for molecular biological analysis and diagnostics |
| US20060257992A1 (en) * | 2004-02-27 | 2006-11-16 | Mcdevitt John T | Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems |
| CN101495869A (en) * | 2005-11-30 | 2009-07-29 | 麻省理工学院 | Pathogen detection biosensor |
| CN103278647A (en) * | 2005-11-30 | 2013-09-04 | 麻省理工学院 | Pathogen detection biosensor |
| CN101036790A (en) * | 2006-03-17 | 2007-09-19 | 四川大学 | Protein genetic vaccines on the surface of mutans streptococci and its preparing method |
| CN101583624A (en) * | 2006-09-29 | 2009-11-18 | 昂考梅德药品有限公司 | Compositions and methods for diagnosing and treating cancer |
| AU2007329748A1 (en) * | 2006-10-25 | 2008-06-12 | Ikonisys, Inc. | Automated detection of cancer and high grade hyperplasias |
| CN101661034A (en) * | 2008-08-28 | 2010-03-03 | 上海领潮生物科技有限公司 | Method for synchronously performing parallel detection of various biomarkers and chip test paper |
| CN107884561A (en) * | 2012-04-04 | 2018-04-06 | 辛辛那提大学 | sweat simulation, collection and sensing system |
| CN105518464A (en) * | 2013-07-05 | 2016-04-20 | 华盛顿大学商业中心 | Methods, compositions and systems for microfluidic assays |
| CN104965096A (en) * | 2015-06-04 | 2015-10-07 | 南京普朗医疗设备有限公司 | Multi-sample immunity-detecting reaction disc capable of automatically separating serum |
| US9700888B1 (en) * | 2016-04-13 | 2017-07-11 | Ipmd Inc. | Point of care medical device for detecting infectious diseases |
| KR101905084B1 (en) * | 2017-07-13 | 2018-10-08 | 농업회사법인 주식회사 스피루리나팜스 | Light cultivating apparatus for culturing microalgae of self-circulating type |
| US20190078982A1 (en) * | 2017-09-14 | 2019-03-14 | Roy G. Whiteside, JR. | Biological fluid testing device |
| CN107805597A (en) * | 2017-09-29 | 2018-03-16 | 深圳国际旅行卫生保健中心 | Gene detection system and detection method based on micro-fluidic chip |
| CN111108394A (en) * | 2017-12-12 | 2020-05-05 | 恩普乐股份有限公司 | Rack for placing analysis plate and analysis kit |
| CN108070641A (en) * | 2018-01-24 | 2018-05-25 | 北京恩泽康泰生物科技有限公司 | It is used to detect the primer and probe of AR-V7 and AR in vesica based on qPCR or digital pcr technology |
| CN111774103A (en) * | 2020-06-01 | 2020-10-16 | 东南大学 | Multi-core spiral inertia separation micro-fluidic device for high-throughput plasma separation |
Non-Patent Citations (4)
| Title |
|---|
| BOYUTANG: "Deletion of cas3 gene in Streptococcus mutans affects biofilm formation and increases fluoride sensitivity", 《ARCHIVES OF ORAL BIOLOGY》 * |
| 刘赵淼等: "微流控液滴技术及其应用的研究进展", 《分析化学》 * |
| 姜云涛: "荧光原位杂交技术和流式细胞仪在菌斑中致龋性变形链球菌定量检测中的应用", 《口腔医学研究》 * |
| 洪潇: "变形链球菌检测技术新进展", 《广东牙病防治》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ludwig et al. | Comparison of expressed prostatic secretions with urine after prostatic massage—a means to diagnose chronic prostatitis/inflammatory chronic pelvic pain syndrome | |
| CN107505295A (en) | A kind of liver cancer monitoring kit and its application method | |
| CN109457032B (en) | Thyroid cancer molecular diagnosis kit | |
| JP4986449B2 (en) | Method for examining floating cells | |
| CN111662982A (en) | Biomarker for early diagnosis and/or recurrence monitoring of brain glioma and application thereof | |
| CN108841949B (en) | Reagent kit and device for early detection and diagnosis of Parkinson's disease | |
| CN112517093A (en) | Fish saliva automatic sample separation detection disc and detection method thereof | |
| JP7187081B2 (en) | Methods for early diagnosis and post-treatment monitoring of breast cancer using liquid biopsy multiplex oncogene biomarkers | |
| CN110023758A (en) | For separating equipment, system, method and the external member of analyte from body fluid sample | |
| CN107513494A (en) | A kind of detection of nucleic acids card and its application method | |
| US20130289443A1 (en) | Fluid Sample Collection and Testing Device and Method | |
| WO2002080775A1 (en) | Method of collecting a gastrointestinal tract sample | |
| KR102016385B1 (en) | Method for Providing Information for Diagnosing Tuberculosis Using GABA as a Biomarker | |
| CN112280665A (en) | Gene detection equipment integrating extraction, amplification and detection | |
| JP2012026997A (en) | Method and instrument for sampling, diluting and dispensing minute specimen by capillary | |
| Yaari et al. | Emerging technologies in cancer detection | |
| CN111638324B (en) | Coronary heart disease diagnosis biomarker composition and application thereof | |
| CN114487215B (en) | Biomarker and kit for detecting benign prostatic hyperplasia | |
| GB2552461A (en) | Method and assay kit to detect an analyte | |
| CN116196042B (en) | Periodontitis detection kit and application thereof | |
| ITMI20001002A1 (en) | METHOD AND EQUIPMENT FOR THE EARLY DIAGNOSIS OF BLADDER TUMORS ON URINE SAMPLES | |
| CN115980368B (en) | Marker group for periodontitis detection | |
| IVATURI et al. | Advanced Chairside Diagnostic Aids for Periodontal Diagnosis-A Review. | |
| Nam et al. | RESEARCH ON THE DETECTION OF HELICOBACTER PYLORI IN SALIVA OF GASTRITIS AND DUODENITIS PATIENTS BY REAL-TIME PCR TECHNIQUE | |
| CN113416780A (en) | Molecular marker for diagnosing and treating cerebral arterial thrombosis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20210319 |
|
| WD01 | Invention patent application deemed withdrawn after publication |