CN112501134B - Human respiratory syncytial virus strain and application thereof - Google Patents

Abstract

The invention discloses a human respiratory syncytial virus strain and application thereof, relating to the field of virus immunity. 4 strains of highly toxic mutant strains of the human respiratory syncytial virus are obtained by continuous passage screening, and all the mutant strains are determined to be A subtype hRSV by gene sequence determination. The highly toxic mutant strain of human respiratory syncytial virus has good genetic stability and strong immunogenicity after immunizing animals. Therefore, the highly toxic mutant strain of the human respiratory syncytial virus can be used for breeding of an attenuated strain, screening the safe and immunocompetent effective attenuated strain, can be applied to infecting mice and causing the mice to die, and thus a lethal hRSV infected animal model is constructed for screening and evaluating hRSV vaccine and anti-hRSV drugs.

Description

Human respiratory syncytial virus strain and application thereof

Technical Field

The invention relates to the field of virus immunity, in particular to a human respiratory syncytial virus strain and application thereof.

Background

At present, human Respiratory Syncytial Virus (hRSV) has no vaccine. The development of vaccines faces the following problems: the age of the infected is generally small (under 5 years), resulting in the use of vaccines with great care; there are various ways for viruses to evade host innate immunity; lack of persistent protective immunity induced by natural infection; in formalin inactivated whole virus vaccines developed in the 60's of the 20 th century, children vaccinated in natural infection have disease aggravation symptoms marked by alveolitis, namely Enhanced RSV Disease (ERD), and the hospitalization rate is obviously increased, even death is caused; in addition, the inability of animal models to fully fit the pathogenesis of viruses in humans has also affected vaccine development.

At present, the only drug licensed to prevent hRSV infection is palivizumab, which is a humanized mouse monoclonal antibody against the virus F protein, and the therapy is safe and effective for premature infants and infants with chronic diseases, but is also very limited in use, and the target for the therapy must be a high risk group (children) with strict indications, and the cost is very high, and the therapy is not applied in China.

In addition, the existing medicament (ribavirin) has the problems of high toxicity, rapid virus variation, increased virus resistance and the like. Modern medicine still cannot develop a safe and effective hRSV vaccine or antiviral drug, and a major bottleneck in development is the lack of a suitable animal model capable of fitting the virus infection in humans. The virus can also cause serious infection and even death of children, old people and adults with low immunity, so that the screening of the high-toxicity mutant strain hRSV is particularly important, and therefore, an animal model of lethal hRSV infection is constructed, and the virus is used for screening and evaluating hRSV vaccines and anti-hRSV drugs.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The invention aims to provide a human respiratory syncytial virus strain and application thereof to solve the technical problems.

The invention is realized by the following steps:

a human respiratory syncytial virus strain, the strain is a subtype A strain, and the human respiratory syncytial virus strain is selected from at least one of the following strains: strain 1, strain 2, strain 3 and strain 4;

the preservation number of the strain 1 is CCTCC NO: v201746; the preservation number of the strain 2 is CCTCC NO: v201755; the preservation number of the strain 3 is CCTCC NO: v202033; the strain 4 has a preservation number of CCTCC NO: v202034.

Aiming at the situation that no human respiratory syncytial virus strain vaccine is published at present, the inventor screens and obtains a high-toxicity mutant strain hRSV. The human respiratory syncytial virus strain comprises 4 strains. 4 strains of virus can cause BALB/c mice to die after being infected, the problem that no lethal animal model is available in the research and development of the hRSV vaccine is solved, and an excellent animal model platform is provided for the screening and evaluation of the hRSV vaccine and the anti-hRSV medicament.

The inventors obtained 4 highly virulent hRSV mutants by serial passage of a clinically isolated hRSV strain (KI strain) in mice after cumulative viral mutations. The KI strain is referred to the isolated human Respiratory Syncytial Virus strain described in Ke Zhang, et al complete genome sequences of one human Respiratory Syncytial Group A viruses from China and its four mouse-adapted isolates.

The screening method of the high-toxicity hRSV refers to the screening of a mouse adaptive strain of the high-toxicity human respiratory syncytial virus and the concrete steps of an animal continuous passage method recorded in the journal of Chinese pathogenic biology.

The 4 strains of hRSV highly toxic mutant strains screened by the inventor all have mutation sites. Specifically, 4 strains of high-toxicity mutant strains of hRSV are all preserved in China Center for Type Culture Collection (CCTCC) at the preservation address of Wuhan university. The strain 1 is preserved with the name of human respiratory syncytial virus A subtype R87-4-18, and the preservation number is CCTCC NO: v201746 with a preservation time of 2019, 1 month and 10 days; the strain 2 is preserved with the name of human respiratory syncytial virus A subtype R88-1-23, and the preservation number is CCTCC NO: v201755 with a preservation time of 2019, 1 month and 10 days; the strain 3 is preserved with the name of human respiratory syncytial virus A subtype R100-3, and the preservation number is CCTCC NO: v202033 with a preservation time of 2020, 9, 21 days; the strain 4 has a preservation name of human respiratory syncytial virus A subtype R100-5, and the preservation number is CCTCC NO: v202034 with a preservation time of 2020, 9, 21 days.

The whole genome sequence of the strain 1 is shown as SEQ ID NO.1, the whole genome sequence of the strain 2 is shown as SEQ ID NO.2, the whole genome sequence of the strain 3 is shown as SEQ ID NO.3, and the whole genome sequence of the strain 4 is shown as SEQ ID NO. 4.

The application of the human respiratory syncytial virus strain in preparing the attenuated strain of the human respiratory syncytial virus. For example, it is expected that the human respiratory syncytial virus strain provided by the invention is used as a mother strain for attenuated strain breeding, and can be used for subsequent attenuated breeding and future mass production, thereby being further used for attenuated live vaccine development. In addition, partial sequences in the strains provided by the invention can be used for developing component vaccines.

The application of the human respiratory syncytial virus strain in preparing the medicines for treating or preventing the respiratory syncytial virus infection.

In one embodiment, the medicament is a vaccine.

In one embodiment, the medicament is a live attenuated vaccine or a protein component vaccine.

A hybridoma cell is prepared by immunizing animals with the human respiratory syncytial virus strain as an immunogen.

The animal is a mouse. In one embodiment, the mouse is a BALB/c mouse.

For example, a mouse is immunized with the human respiratory syncytial virus strain, myeloma cells are fused with immune B lymphocytes of the immunized mouse, and hybridoma cells are cultured in a medium.

An antiserum, which is prepared by immunizing animals by taking the human respiratory syncytial virus strain as an immunogen. For example, after subcutaneous immunization of an animal, blood is collected from the carotid artery and/or fundus of the animal and serum is collected.

Preferably, the animal is a mouse, monkey, rabbit or sheep. In other embodiments, the above-mentioned immunized animal may be selected from a horse, a chicken, a donkey, or a pig. In one embodiment, the mouse is a BALB/c mouse.

An antibody, which is prepared by taking the human respiratory syncytial virus strain as an immunogen to immunize animals. The animal is mouse, monkey, rabbit or sheep. In other embodiments, the above-mentioned immunized animal may be selected from horses, chickens, donkeys or pigs. In one embodiment, the mouse is a BALB/c mouse.

The invention has the following beneficial effects:

the invention obtains 4 strains of human respiratory syncytial virus high-toxicity mutant strains through continuous passage screening, and all the mutant strains are A subtype hRSV determined by gene sequence determination. The highly toxic mutant strain of human respiratory syncytial virus has good genetic stability and strong immunogenicity after immunizing animals. Therefore, the highly toxic mutant strain of the human respiratory syncytial virus can be used for breeding attenuated strains, screening the attenuated strains with safety and effective immunocompetence, can be applied to infect mice and cause the mice to die, and is used for constructing a lethal hRSV infected animal model for screening and evaluating hRSV vaccines and anti-hRSV medicines.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a schematic view of the operation of continuous subculture;

FIG. 2 shows the body weight change and survival of mice infected with HRSV-A-GZ08-0 (wild strain), HRSV-A-GZ08-18 (highly virulent strain) and R87-4-18 (highly virulent strain);

FIG. 3 is Sup>A diagram showing the genomic alignment of R87-4-18 (Sup>A highly virulent strain) with HRSV-A-GZ 08-18;

FIG. 4 shows the change in body weight and survival of mice infected with R88-1-23;

FIG. 5 is Sup>A diagram of the genomic alignment of R88-1-23 with HRSV-A-GZ 08-18;

FIG. 6 shows the body weight change and survival of mice infected with R100-3;

FIG. 7 is Sup>A graph of the genomic alignment of R100-3 with HRSV-A-GZ 08-18;

FIG. 8 shows the body weight change and survival of mice infected with R100-5;

FIG. 9 is Sup>A diagram showing the genomic alignment of R100-5 with HRSV-A-GZ08-18.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The features and properties of the present invention are described in further detail below with reference to examples.

Example 1

And (3) inoculating the KI strain into a mouse, and continuously carrying out subculture. The KI strain is a strain of human respiratory syncytial virus clinically isolated from Guangzhou children hospital in 2008. The continuous subculture method refers to a flow chart shown in FIG. 1, and specific steps refer to screening of mouse adapted strains of the high-toxicity human respiratory syncytial virus and specific steps of an animal continuous subculture method recorded in the journal of Chinese pathogenic biology.

(1) Culture of hRSV in human HEp-2 cells: the virus solution is diluted in DMEM/F12-GlutaMAX according to the volume ratio of 1 ^TM Spreading the culture solution on pre-inoculated HEp-2 cells, culturing at 37 deg.C in a CO2 cell culture box at 5% for 1h, and gently shaking the culture bottle every 15min to prevent the cells from drying up; discarding the virus solution after 1h, adding DMEM/F12-GlutaMAX containing 2% FBS ^TM And (5) continuously culturing the culture solution for 3-5d, observing cytopathic effect (CPE) every day, evaluating the change of virus titer, and stopping virus culture at proper time. The virus supernatant was collected and centrifuged to remove cell debris for inoculation into mice.

(2) Tracheal inoculation of 100. Mu.l hRSV into 8-month-old BALB/c miceAnd on the same day with TCID ₅₀ The method determines the titer of the inoculated virus and records the body weight and clinical signs of the mice day by day.

(3) Three days later, mice were sacrificed and DMEM/F12-GlutaMAX containing vancomycin, amikacin, ciprofloxacin, nystatin, penicillin and streptomycin ^TM Culture fluid lavage of both lungs of mice, TCID ₅₀ The method and RT-Q-PCR method were used to detect viral titers in lung lavage fluid, while culturing hRSV in lung lavage fluid with HEp-2 cells, and if animals scored more than 16 points (shown in Table 1), the animals were tried to inoculate the next round of mice directly with lung lavage fluid.

TABLE 1 Scoring disease status after Virus infection in mice

(4) Recording TCID five days later ₅₀ Results of the method, compared with the results of the virus titer and RT-Q-PCR, the results of the passage of the virus in the animal lung were evaluated, and a new round of virus passage was continued using hRSV virus in lung lavage fluid cultured with HEp-2 cells as a source.

(5) Repeating (2) - (4) and continuously carrying out passage 60.

(6) For virus strains with special phenotypes (such as mice with obvious clinical symptoms, asthma, immobility, limb paralysis and the like) or virus strains with very high titer, mouse adapted strains are obtained by separation and purification of a virus Plaque test (Plaque assay), and are cultured by HEp-2 cells for whole genome sequencing.

(7) The obtained mouse adapted strain was inoculated to a mouse, correlation between clinical symptoms of the mouse and the base mutation was observed, and correlation between the viral titer in the mouse lung and the base mutation was determined.

Experimental example 1

This example provides the body weight change and survival of mice infected with HRSV-A-GZ08-0 (wild strain), HRSV-A-GZ08-18 (highly virulent strain) and R87-4-18 (highly virulent strain) (see FIG. 2). HRSV-A-GZ08-0 (wild strain) is Sup>A KI strain which is clinically isolated from Guangzhou children hospital in 2008, and the NCBI sequence number is as follows: KP218910.HRSV-A-GZ08-18 is Sup>A high-strain obtained by continuous passage of Sup>A KI strain, and the NCBI has Sup>A sequence number as follows: KP119747.

HRSV-A-GZ08-18 is described in published articles: complete genome sequences of one human resource analytical Group A viruses from Chinese and its four mouse-adapted oligonucleotides genome Annonanc 3 (2) e00062-15. Doi.

As can be seen from FIG. 2, the highly toxic mutant strain R87-4-18 obtained by screening can obviously reduce the body weight of the mouse within 7d after the mouse is infected, and the lethality rate is obviously higher than that of HRSV-A-GZ08-18.

It should be noted that R87-4-18 of 2 doses shown in FIG. 2 can significantly reduce the body weight of mice within 7d of infected mice and can also kill the mice; 5 doses of RSV-A-GZ08-18 can kill mice, but less than 4 doses of HRSV-A-GZ08-18 can not kill mice, so the high-toxicity mutant strain R87-4-18 obtained by screening has better lethality rate than HRSV-A-GZ08-18.

The sequence of R87-4-18 (CCTCC NO. V201746) is compared with HRSV-A-GZ08-18, and the similarity is up to 99 percent (refer to the figure 3).

Experimental example 2

The experimental example provides the body weight change and survival condition of mice infected with HRSV-A-GZ08-0 (wild strain), HRSV-A-GZ08-18 (highly virulent strain) and R88-1-23 (highly virulent strain). HRSV-A-GZ08-0 (wild strain) and HRSV-A-GZ08-18 were derived from the same sources as in Experimental example 1.

Referring to FIG. 4, it can be seen from FIG. 4 that the highly toxic mutant strain R88-1-23 (2 doses) obtained by screening can significantly reduce the body weight of the mice within 7d after the mice are infected, and the lethality rate is obviously higher than that of HRSV-A-GZ08-18.

It should be noted that R88-1-23 of 2 doses shown in FIG. 4 can significantly reduce the body weight of mice within 7d of infected mice, and can also kill the mice; 5 dose RSV-A-GZ08-18 can kill mice, but less than 4 dose HRSV-A-GZ08-18 can not kill mice, so the lethality rate of the highly toxic mutant strain R88-1-23 screened by the invention is better than that of HRSV-A-GZ08-18.

The sequence of R88-1-23 (CCTCC NO. V201755) is compared with HRSV-A-GZ08-18, and the similarity is up to 99 percent (shown in figure 5).

Experimental example 3

The experimental example provides the body weight change and survival condition of mice infected with HRSV-A-GZ08-0 (wild strain), HRSV-A-GZ08-18 (highly virulent strain) and R100-3 (highly virulent strain). HRSV-A-GZ08-0 (wild strain) and HRSV-A-GZ08-18 were derived from the same sources as in Experimental example 1.

Referring to FIG. 6, it can be seen from FIG. 6 that the highly toxic mutant strain R100-3 obtained by screening can significantly reduce the body weight of mice within 7d after the mice are infected, and the lethality rate is obviously higher than HRSV-A-GZ08-18.

It should be noted that R100-3 of 2 doses shown in FIG. 6 can significantly reduce the weight of the mice within 7d of infected mice, and can also kill the mice; RSV-A-GZ08-18 with 5 doses can kill mice, but HRSV-A-GZ08-18 with less than 4 doses can not kill mice, so the lethality rate of the high-toxicity mutant strain R100-3 obtained by screening is better than that of HRSV-A-GZ08-18.

The sequence of R100-3 (CCTCC NO. V202033) was aligned with HRSV-A-GZ08-18, and the degree of similarity was as high as 99% (see FIG. 7).

Experimental example 4

The experimental example provides the body weight change and survival condition of mice infected with HRSV-A-GZ08-0 (wild strain), HRSV-A-GZ08-18 (highly virulent strain) and R100-5 (highly virulent strain). HRSV-A-GZ08-0 (wild strain) and HRSV-A-GZ08-18 were derived from the same sources as in Experimental example 1.

Referring to FIG. 8, it can be seen from FIG. 8 that the highly toxic mutant strain R100-5 obtained by screening can significantly reduce the body weight of the mice within 7d after the mice are infected, and the lethality rate is obviously higher than that of HRSV-A-GZ08-18.

It should be noted that R100-5 of 2 doses shown in FIG. 8 can significantly reduce the body weight of mice within 7d of infected mice, and can also kill the mice; 5 dose RSV-A-GZ08-18 can kill mice, but less than 4 dose HRSV-A-GZ08-18 can not kill mice, so the lethality rate of the high-toxicity mutant strain R100-5 obtained by screening is better than that of HRSV-A-GZ08-18.

The sequence of R100-5 (CCTCC NO. V202034) was aligned with HRSV-A-GZ08-18, and the degree of similarity was as high as 99% (see FIG. 9).

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

SEQUENCE LISTING

<110> affiliated hospital of Guizhou medical university

<120> human respiratory syncytial virus strain and application thereof

<160> 4

<170> PatentIn version 3.5

<210> 1

<211> 15173

<212> DNA

<213> Artificial sequence

<400> 1

cgtacaacaa acttgcgtaa accaaaaaag tggggcaaat aagaatttga taagtaccac 60

ttaaatttaa ctcctttggt tagagatggg cagcaactca ttgagtatga taaaagttag 120

attgcaaaat ttatttgaca atgatgaagt agcattgtta aaaataacat gctatactga 180

caaattaata cagttaatta atgctttggc taaggcagtt atacatacaa tcaaattgaa 240

tggcatagta tttgtgcatg ttattacaag tagtgatatt tgccctaata ataatatagt 300

agtgaaatcc aatttcacaa caatgccagt attacaaaat ggaggttata tatgggaaat 360

gatggaatta acacactgct ctcaacctaa tggcctaata gatgacaatt gtgaaattaa 420

attctccaaa aactaagtga ttcaacaatg accaattata tgaatcaatt atctgaatta 480

cttggatttg accttaatcc ataaattata ataaatatca actagaaaat caatgtcact 540

aacaccatta gttaatataa aacttgacag aagataagaa tggggcaaat aaatcaactc 600

agccgaccca accatggaca caacacacaa tgataccaca ccacaaagac tgatgatcac 660

agacatgaga ccattgtcac ttgagactat aataacatca ctaaccagag acatcataac 720

acatagattt atatacttga taaatcatga atgcatagtg agaaaacttg atgaaagaca 780

ggccacattt acattcctgg tcaactatga aatgaaacta ttgcacaaag tgggaagcac 840

taaatataaa aaatatactg aatacaacac aaaatatggc actttcccta tgccaatatt 900

tatcaatcat gatgggttct tagaatgcat tggcattaag cctactaagc acacacccat 960

aatatacaag tatgatctca atccatgaat tttaaaacaa gattcaaaca atccgaaata 1020

acaacttcat gcataatcac actccatagt ccaaatggag cctgaaaatt atagtaattt 1080

aaaattaagg agagacataa gatgaaagat ggggcaaata caaaaatggc tcttagcaaa 1140

gtcaagttga atgatacact caacaaagat caacttctgt catccagcaa atacaccatc 1200

caacggagca caggagacgg cattgacact cctaattatg atgtgcagaa acacatcaat 1260

aagctatgtg gcatgttatt aatcacagaa gatgctaatc ataaattcac tgggttaata 1320

ggtatgttat atgctatgtc tagattagga agagaagaca ccataaaaat actcaaagat 1380

gcgggatatc atgttaaagc aaatggagtg gatgtaacaa cacatcgtca agacattaat 1440

gggaaagaaa tgaaatttga agtgttaaca ttagcaagct taacaactga aattcaaatc 1500

aacattgaga tagaatctag aaaatcctac aaaaaaatgc taaaagaaat gggagaggtg 1560

gctccagaat acaggcatga ctctcctgat tgtgggatga taatattatg tatagcggca 1620

ttagtaataa ccaaattagc agcaggggat agatctggtc ttacagctgt gattaggaga 1680

gctaataatg tcctaaaaaa tgaaatgaaa cgttataaag gcttactacc caaggatata 1740

gccaacagct tctatgaagt gtttgaaaaa tatcctcact ttatagatgt ttttgttcat 1800

tttggtatag cacaatcttc taccagaggt ggcagtagag ttgaagggat ttttgcagga 1860

ttgtttatga atgcttatgg tgcagggcaa gtgatgttac ggtggggggt cttagcaaaa 1920

tcagttaaaa atattatgtt aggacacgct agtgtgcaag cagaaatgga acaagttgtg 1980

gaggtgtatg aatatgccca aaaattgggt ggagaagcag gattctacca tatattgaac 2040

aacccaaaag catcattatt atctttgact caatttcctc acttctctag tgtagtatta 2100

ggcaatgctg ctggcctagg cataatggga gaatacagag gtacaccaag gaaccaagat 2160

ctatatgatg ctgcaaaagc atatgctgaa caactcaaag aaaatggtgt gattaactac 2220

agtgtattag acttgacagc agaagagcta gaggctatca aacatcagct taatccaaaa 2280

gataatgatg tagagctttg agttaataaa aaagtggggc aaataaatca tcatggaaaa 2340

gtttgctcct gaattccatg gagaagatgc aaacaacaga gccaccaaat ttctagaatc 2400

aataaagggc aaattcacat cacccaaaga tcccaagaaa aaagatagta tcatatctgt 2460

caactcaata gatatagaag taaccaaaga aagccctata acatcaaatt caaccattat 2520

aaacccaata aatgagacag atgatactgt agggaacaag cccaattatc aaagaaaacc 2580

tctagtaagt ttcaaagaag atcctacgcc aagtgataat cccttttcaa aactatacaa 2640

agaaaccata gaaacatttg ataacaatga agaagaatct agctattcat atgaagaaat 2700

aaatgatcag acaaacgata atataacagc aagattagat aggattgatg agaaattaag 2760

tgaaatactg ggaatgcttc acacattagt agtagcgagt gcaggaccca catctgctcg 2820

ggatggtata agagatgcca tggttggttt aagagaagaa atgatagaaa aaatcagaac 2880

tgaagcatta atgaccaatg acagactaga agctatggca agactcagga atgaggaaag 2940

tgaaaagatg gcaaaagaca catcagatga agtgtctctc aatccaacat cagagaaatt 3000

gaacaacctg ttggaaggga atgatagtga caatgatcta tcacttgaag atttctgatt 3060

agctaccaat ctgcacatca aaacacaaca ccaatagaag aacaacaaac aaaccaactc 3120

acccatccaa ccaaacatct atctgccgat tagccaacca gccaaaaaaa caaccagcta 3180

atccaaaact agccacccgg ataatatcaa tactatagtt acaaaaaaag atggggcaaa 3240

tatggaaaca tacgtgaaca aacttcacga gggctccaca tacacagctg ctgttcaata 3300

caatgtccta gaaaaagacg atgatcctgc atcacttaca atatgggtgc ccatgttcca 3360

atcatccatg ccagcagatt tacttataaa agaactagct aatgtcaata tactagtgaa 3420

acaaatatcc acactcaagg gaccctcatt aagagtcatg ataaactcaa gaagtgcagt 3480

actagcacaa atgcccagca aatttaccat atgtgccaat gtgtccttgg atgaaagaag 3540

caagctggca tatgatgtaa ccacaccctg tgaaattaag gcatgcagtc taacatgcct 3600

aaaatcagaa aatatgttaa ctacagtcaa agatctcacc atgaaaacac tcaacccaac 3660

acatgacatc attgctttat gtgaatttga aaatatagta acatcaaaaa aagtcataat 3720

accaacatac ctaagatcca tcagtgtcag aaataaagat ctgaacacac ttgaaaatat 3780

aacaaccact gaattcaaaa atgccattac aaatgcaaaa atcatccctt actcaggatt 3840

actgttagtc atcacagtga ctgacaacaa aggagcattc aaatacataa agccacaaag 3900

tcaattcata gtagatcttg gagcttacct agaaaaagaa agtatatact atgttacaac 3960

aaattggaag cacacagcta cacgatttgc aattaaaccc atggaagatt aaccttttct 4020

ctctacatca gtgagttgat tcatacaaac tttctactta cattcctcac cacacaatca 4080

taatcaccaa ctctctgtgg ttcaaccaat caaataaaat tcatcaagag tctcagatca 4140

tcccaaatca ttgttcatca gatccagtac tcaaataagt taataaaaaa tccacatggg 4200

gcaaataatc attgagggaa atccaaccaa tcacaacatc tgtcaacata gacaagtcaa 4260

cacgttagat aaaatcaacc aatggaaaat acatccataa caatagaatt ctcaagcaaa 4320

ttctggcctt actttacact aatacacatg ataacaacaa taatctcttt gctaatcata 4380

atctccatca tgattgcaat actaaacaaa ctctgcgaat ataatgtatt tcataacaaa 4440

acctttgagc taccaagagc ccgagtcaat acatagcatt caccaatctg atggctcaaa 4500

acagtaacct tgcatttgta agtgaactac catcacctct tcacaaaacc acatcaacat 4560

ctcaccatgc aagccatcat ctataccata aagtagttaa ttaaaaatag tcataacaat 4620

gaactaagat attaagacca acaacaacgt tggggcaaat gcaaacatgt ccaaaaccaa 4680

ggaccaacgc accgccaaga cactagaaag gacctgggac actcttaatc atctattatt 4740

catatcatcg tgcttataca agttaaatct taaatctata gcacaaatca cattatctat 4800

tttggcaatg ataatctcaa cttcacttat aattgcagcc atcatattca tagcctcggc 4860

aaaccacaaa gtcacactaa caactgcaat catacaagtt gcaacaaacc aggtcaagaa 4920

cacaacccca acatacctca cccagaatcc ccagcttgga atcagcttct ccaatctgtc 4980

cgaaactaca tcacaaccca ccaccatact agcttcaaca acaccaagtg ctgagtcaac 5040

cccacgatcc acaacagtca agaccaaaaa cacaacaaca acccaaatac aacctagcaa 5100

gtccaccaca aaacaacacc aaaacaaacc acaaaacaaa cccaataatg attttcactt 5160

tgaagtgctc aactttgtac cctgcagcat atgcagcaac aatccaactt gctgggctat 5220

ctgcaaaaga ataccaaaca aaaaacttgg agagaaaacc accaccaagc ccacaaaaaa 5280

accaaccacc aagacaacca aaaaagatct caaacctcaa accacaaaac caaaggaagt 5340

acttaccaca aagcccacag aaaagcaaac catcaacacc accaaaacaa acatcagaac 5400

tacactgctc acctccaaca ccacaggaaa tccagaacac acaagtcaaa aggaaaccct 5460

ccactcaacc acctccgaag gcaatccaag cccctcacaa gtctatacaa catccgagta 5520

cctatcacaa tctctatctc catccaacac aacatactag tagtcattaa aaagtgtatt 5580

attgcaaaaa accataacca aatcaaacag aatcaaaatc aactctgggg caaataacaa 5640

tggagttgcc aatcctcaaa gcaaatgcta ttaccacaat ccttgctgca gtcacactct 5700

gttttgcttc cagtcaaaac atcactgaag aattttatca atcaacatgc agtgcagtta 5760

gcaaaggcta tcttagtgct ctaagaactg gttggtatac tagtgttata actatagaat 5820

taagtaatat caaggaaaat aagtgtaatg gaacagatgc taaggtaaaa ttgataaaac 5880

aagaattaga taaatacaaa aatgctgtaa cagaattgca gttgctcatg caaagcacac 5940

cagcagccaa caatcgagcc agaagggaat taccaagatt tatgaattat acactcaaca 6000

atactgaaaa caccaatgta acattaagca agaaaaggaa aagaagattt cttggctttt 6060

tgttaggtgt tggatctgca atcgccagtg gcattgccgt atccaaggtc ctgcacctag 6120

aaggggaagt gaacaaaatc aaaagtgctc tactatccac aaacaaggct gtagtcagct 6180

tatcaaatgg agtcagtgtc ttaaccagca aagtgttaga tctcaaaaac tatatagata 6240

aacagttgtt acctattgtg aacaagcaaa gctgcagcat atcaaacatt gaaactgtaa 6300

tagagttcca acaaaagaac aacagactac tagagattac cagagaattt agtgttaatg 6360

caggtgtaac tacacctgta agcacttata tgttaactaa tagtgaatta ttatcattaa 6420

tcaatgatat gcctataaca aatgatcaga aaaagttaat gtccaacaat gttcaaatag 6480

ttagacagca aagttactct atcatgtcca taataaaaga ggaagtctta gcatatgtag 6540

tacaattacc actatatggt gtaatagata caccttgttg gaaactgcac acatcccctc 6600

tatgtacaac caacacaaag gaagggtcca acatctgctt aacaagaacc gacagaggat 6660

ggtactgtga caatgcagga tcagtatctt tcttcccaca agccgaaaca tgtaaagttc 6720

aatcgaatcg ggtattttgt gacacaatga acagtttaac attaccaagt gaggtaagtc 6780

tctgcaacgt tgacatattc aaccccaaat atgattgcaa aattatgact tcaaaaacag 6840

atgtaagcag ctccgttatc acatctctgg gagccattgt gtcatgctat ggcaaaacca 6900

aatgtacagc atccaataaa aatcgtggga tcataaagac attttctaac gggtgtgatt 6960

atgtatcaaa taagggggtg gatactgtgt ctgtaggtaa tacattatat tatgtaaata 7020

agcaagaagg caaaaatctc tatgtaaaag gtgaaccaat aataaatttc tatgacccat 7080

tagtgttccc ctctgatgaa tttgatgcat caatatctca agtcaatgag aagattaacc 7140

agagtctagc atttattcgt aaatcagatg aattattaca taatgtaaat gctgttaaat 7200

ccactacaaa tatcatgata actactataa ttatagtgat tatagtaata ttgttatcat 7260

taattgtagt tggactgctt ctatactgca aggccagaag cacaccagtc acactaagta 7320

aggatcaact gagtggtata aataatattg catttagtag ctgaataaaa ttagcaccta 7380

atcatattct tacaatggtt cactatctga ccatagataa cccatctatc attggatttt 7440

cttaaaattt gaacttcatc acaactttca tctataaacc atctcactta cactatttaa 7500

gtagattcct attttatagt tatataaaac aattgaatac cagattaact tactatttgt 7560

aaaaataaga actggggcaa atatgtcacg aaggaatcct tgcaaatttg aaattcgagg 7620

tcattgcttg aatggtaaga ggtgtcattt tagtcataat tattttgaat ggccacccca 7680

tgcactgctt gtaagacaaa actttatgtt aaacagaata cttaagtcta tggataaaag 7740

catagatact ttatcagaaa taagtggagc tgcagagttg gacagaacag aagagtatgc 7800

cctcggtgta gttggagtgc tagaaagtta tataggatca ataaataata taactaaaca 7860

atcagcatgt gttgccatga gcaaactcct cactgaactc aacagtgatg acatcaaaaa 7920

actaagagac aatgaagagc caaattcacc caagataaga gtgtacaata ctgtcatatc 7980

atatattgaa agcaacagga aaaacaataa acaaactatc catctgttaa aaagattgcc 8040

agcagacgta ttgaagaaaa ccatcaaaaa cacattggat atccacaaga gcataaccat 8100

caataaccca aaagaatcaa ctgttagtga tacgaacgac catgccaaaa ataatgatac 8160

tacctgacaa atatccttgt agtataaatt ccatactaat aacaagtagt tgtagagtta 8220

ctatgtataa tcaaaaaaac acactatata tcgatcaaaa caaccaaaat aaccttatat 8280

actcaccgaa tcaaccattc aatgaaatcc attggacctc tcaagacttg attgatgcaa 8340

ctcaaaattt tctacaacat ctaggtatta ctgatgatat atacacaata tatatattag 8400

tgtcataaca ctcaatccta atacttacca catcatcaaa ttattaactc aaacaattca 8460

agctatggga caaaatggat cccattatta atggaaattc tgctaatgtt tatctaactg 8520

atagttattt aaaaggtgtt atttctttct cagaatgtaa cgctttagga agttacatac 8580

tcaatggtcc ttatctcaaa aatgattata ccaacttaat tagtagacaa aatccattaa 8640

tagaacacat aaatctaaaa aaactaaata taacacaatc cttaatatct aagtatcata 8700

aaggtgaaat aaaaatagaa gaacctactt attttcagtc attacttatg acatacaaga 8760

gtatgacctc gtcagaacag attactacca ctaatttact taaaaagata ataagaagag 8820

ctatagaaat tagtgatgtc aaagtctatg ctatattgaa taaactgggg cttaaagaga 8880

aagacaagat taaatccaac aatggacaag atgaagacaa ctcagttatt acaaccataa 8940

tcaaagatga tatactttta gctgttaaag ataatcaatc tcatcttaaa gcagacaaaa 9000

atcactctac aaaacaaaaa gatacaatca aaacaacact cttgaagaaa ttaatgtgtt 9060

cgatgcaaca tcctccatca tggttaatac attggtttaa tttatacaca aaattaaaca 9120

gcatattaac acagtatcga tctagtgagg taaaaaacca tggttttata ttgatagata 9180

atcatactct taatggattc caatttattt tgaatcaata tggttgtata gtttatcata 9240

aggaactcaa aagaattact gtgacaacct ataatcaatt cttgacatgg aaagatatta 9300

gccttagtag attaaatgtt tgtttgatta catggattag caactgtttg aacacattaa 9360

ataaaagctt aggcttaaga tgtggattca ataatgttat cttgacacaa ttattccttt 9420

atggagattt tatactaaaa ctatttcaca atgaggggtt ctacataata aaagaggtag 9480

agggatttat tatgtctcta attttaaata taacagaaga agatcaattc agaaaacggt 9540

tttataatag tatgctcaac aacatcacag atgctgctaa taaagctcag aaaaatctgc 9600

tatcaagagt atgtcataca ttattagata agacagtatc cgataatata ataaatggca 9660

gatggataat tctattaagt aagttcctta aattaattaa gcttgcaggt gacaataacc 9720

ttaacaatct gagtgaattg tattttttgt tcagaatatt tggacaccca atggtagatg 9780

aaagacaagc catggatgct gttaaagtta attgcaacga gaccaaattt tacttgttaa 9840

gcagtttaag tatgttaaga ggtgccttta tatatagaat tataaaaggg tttgtaaata 9900

attacaacag atggcctact ttaaggaatg ccattgtttt acccttaaga tggttaactt 9960

actataaact aaacacttat ccttccttgt tggaacttac agaaagagat ttgattgttt 10020

tatcaggact acgtttttat cgagagtttc ggttgcctaa aaaagtggat cttgaaatga 10080

tcataaatga taaggctata tcacctccta aaaatttaat atggactagt ttccctagaa 10140

attatatgcc gtcacacata caaaattata tagaacatga aaaattaaaa ttctctgaga 10200

gtgataaatc aagaagagta ttagagtact atttaagaga taacaaattc aatgaatgtg 10260

gtttatacaa ctgtgtagtt aatcaaagtt atcttaacaa ccctaatcat gtggtatcat 10320

tgacaggcaa agaaagagaa ctcagtgtag gtagaatgtt tgcaatgcaa ccaggaatgt 10380

tcagacaagt tcaaatatta gcagaaaaat tgatagctga aaacatttta caatttttcc 10440

ctgaaagtct tacaagatat ggtgatctag aactacagaa aatattagaa ttgaaagcag 10500

gaataagtaa caaatcaaat cgatacaatg ataattacaa caattacatt agtaagtgct 10560

ctatcatcac agatctcagc aaattcaatc aagcatttcg atatgaaaca tcatgtattt 10620

gtagtgatgt actggatgaa ctgcatggtg tacaatctct attttcctgg ttacatttaa 10680

ctattcctca tgccacaata atatgcacgt ataggcatgc acccccctat ataagggatc 10740

atattgtaga tcttaacaat gtagatgagc aaagtggatt atatagatat catatgggtg 10800

gtatcgaagg gtggtgtcaa aaactatgga ccatagaagc tatatcacta ttagatctaa 10860

tatctctcaa agggaaattc tcaattactg ctttaattaa tggtgacaat caatcaatag 10920

atataagtaa accagtcaga ctcatggaag gtcaaactca tgctcaagca gattatttgc 10980

tagcattaaa tagtcttaaa ttactgtata aagagtatgc aggcataggc cacaaattaa 11040

aaggaactga gacttatata tcaagagata tgcaatttat gagtaaaacg atccaacata 11100

acggtgtata ttacccagct agtataaaga aagtcctaag agtggggccg tggataaaca 11160

ctatacttga tgatttcaaa gtgagtctag aatctatagg tagtttgaca caggaattag 11220

aatatagagg tgaaagtcta ttatgcagtt taatatttag aaatgtatgg ttatataatc 11280

aaattgcttt acaactaaaa aatcatgcat tatgtaacaa caaattatat ttagacatat 11340

taaaggttct aaaacactta aaaacctttt ttaatcttga taatattgat acagcattaa 11400

cattgtatat gaatttgccc atgttatttg gtggtggtga tcccaacttg ttatatcgaa 11460

gtttctatag aagaactcct gatttcctca cagaggctat agttcactct gtgttcatac 11520

ttagttatta tacaaaccat gatttaaaag ataaacttca agatctgtca gatgatagat 11580

tgaataagtt cttaacatgc ataatcacgt ttgacaaaaa ccctaatgct gaattcgtaa 11640

cactgatgag agatcctcaa gctttaggat ctgagaggca agctaaaatt actagcgaaa 11700

tcaatagact ggcagttact gaggttttga gcacagctcc aaacaaaata ttctccaaaa 11760

gtgcacaaca ctatacaact acagagatag atcttaatga tattatgcaa aatatagaac 11820

ctacatatcc tcacgggcta agagttgttt atgaaagttt acccttttat aaagcagaga 11880

aaatagtaaa tcttatatcc ggtacaaaat ctataactaa catactggaa aagacttctg 11940

ccatagactt aacagatatt gatagagcca ctgagatgat gaggaaaaac ataactttgc 12000

ttataaggat attaccatta gattgtaaca aagataaaag agaaatattg agtatggaaa 12060

acctaagtat tactgaatta agcaaatatg ttagagaaag atcttggtct ttatctaata 12120

tagttggtgt tacatcaccc agtatcatgt atacaatgga catcaaatat acaacaagca 12180

ctatagctag tggcataatc atagagaaat ataatgtcaa cagtttaaca cgtggtgaga 12240

gaggacccac taaaccatgg gttggttcat ctacacaaga gaaaaaaaca atgccagttt 12300

ataatagaca agttttaacc aaaaaacaga gagatcaaat agatctatta gcaaaattgg 12360

attgggtgta tgcatctata gataacaagg atgaattcat ggaggaactt agcataggaa 12420

ctcttgggtt aacatatgag aaagccaaaa aattatttcc acaatattta agtgttaact 12480

atttgcatcg tcttacagtc agtagtagac catgtgaatt ccctgcatca ataccagctt 12540

atagaactac aaattaccac tttgatacta gccctattaa tcgcatatta acagaaaagt 12600

atggtgatga agatattgat atagtattcc aaaactgtat aagctttggc cttagcttaa 12660

tgtcagtagt agaacaattt actaatgtat gtcctaacag aattattctt atacccaagc 12720

ttaatgagat acatttgatg aaaccaccta tattcacagg tgatgttgat attcacaagt 12780

taaaacaagt gatacaaaaa cagcatatgt ttttaccaga caaaataagt ttgactcaat 12840

atgtggaatt attcttaagt aataaaacac tcaaatctgg atctaatgtt aattctaatt 12900

taatattggc acataagata tctgactatt ttcataatac ttacatttta agtactaatt 12960

tagctgggca ttggattctg attatacaac ttatgaaaga ttctaagggt atttttgaaa 13020

aagattgggg agagggatat ataactgatc atatgttcat taatttgaaa gttttcttca 13080

atgcttataa gacatatctc ttgtgttttc ataaaggtta cggcagagca aagctggaat 13140

gtgatatgaa tacttcagat ctcctatgtg tattggaatt aatagacagt agttattgga 13200

agtctatgtc taaggtattt ttagaacaaa aagttatcaa atacattctt agccaggatg 13260

caagtttaca tagagtaaaa ggatgtcata gcttcaaatt atggttcctt aaacgcctta 13320

atgtagcaga attcacagtt tgcccttggg ttgttaacat agattatcat ccaacacata 13380

tgaaagcaat attaacttat atagatcttg ttagaatggg attgataaat atagatagaa 13440

tatacattaa aaataaacac aaattcaatg atgaatttta tacttctaat cttttttaca 13500

ttaattataa cttctcagat aatactcatc tattaactaa acatataagg attgctaatt 13560

ctgaattaga aagtaattac aacaaattat atcatcctac accagaaacc ctagaaaata 13620

tactaaccaa tccagttaaa agtaatgata aaaagacact aagtgactat tgtataggta 13680

aaaatgttga ctcaataatg ttaccattgt tatctaataa gaagcttatt aaatcgtcta 13740

caatgattag aaccaattac agcagacaag atttgtataa tttatttcct acggttgtga 13800

ttgataaaat tatagatcat tcaggtaata cagccaaatc taaccaactt tacactacta 13860

cttctcatca aatatcttta gtgcacaata gcacatcact ttattgcatg cttccttggc 13920

atcacattaa tagattcaat tttgtattta gttctacagg ttgtaaaatt agtatagagt 13980

atattttaaa agaccttaaa attaaagatc ctaattgtat agcattcata ggtgaaggag 14040

cagggaattt attattgcgt acagtagtgg aacttcatcc tgatataaga tatatttaca 14100

gaagtctgaa agattgcaat gatcatagtt taccaattga gtttttaagg ctgtacaatg 14160

gacatatcaa cattgattat ggtgaaaatt tgaccattcc tgctacagat gcaaccaaca 14220

acattcattg gtcttattta catataaagt ttgctgaacc tatcagtctt tttgtctgtg 14280

atgctgaatt gcctgtaaca gtcaattgga gtaaaattat aatagagtgg agcaagcatg 14340

taagaaaatg caaatattgt tcttcagtta ataaatgtac gttaatagta aaatatcatg 14400

ctcaagatga tatcgatttc aaattagaca acataactat attaaaaact tatgtatgct 14460

taggcagtaa gttaaaggga tctgaagttt acttagtcct tacaataggt cctgcaaata 14520

tgttccctgt atttgatgta gtacaaaatg ctaaattgat actatcaaga accaaaaatt 14580

tcatcatgcc taagaaggct gataaagagt ctattgatgc aaatattaag agtttgatac 14640

cctttctttg ttaccctata acaaaaaaag gaataaatac tgcattgtct aaactaaaga 14700

gtgttgttag tggagatata ctatcatatt ctatagctgg acgcaatgaa gttttcagca 14760

ataaacttat aaatcataag catatgaaca tcttaaagtg gttcaatcat gttttaaatt 14820

tcagatcaac agaattaaac tataatcatt tatatatggt agaatctaca tatcctcatc 14880

taagtgaatt gttaaacagc ttgacaacca atgaacttaa aaaactgatt aaaatcacag 14940

gtagtttgtt atacaacttt cataatgaat aatgaacaaa aatcttataa taaaaattcc 15000

catagctaca cactaacact gtattcaatt atagttattt aaaattaata attatataat 15060

ttttttaata acttctagtg aactaatcct aaaattatca ttttgatcta gaaggaataa 15120

atttaaatcc aaatctaatt ggcttatatg tatattaact aaactacgag ata 15173

<210> 2

<211> 15167

<212> DNA

<213> Artificial sequence

<400> 2

caacaaactt gcgtaaacca aaaaagtggg gcaaataaga atttgataag taccacttaa 60

atttaactcc tttggttaga gatgggcagc aactcattga gtatgataaa agttagattg 120

caaaatttat ttgacaatga tgaagtagca ttgttaaaaa taacatgcta tactgacaaa 180

ttaatacagt taattaatgc tttggctaag gcagttatac atacaatcaa attgaatggc 240

attgtatttg tgcatgttat tacaagtagt gatatttgcc ctaataataa tatagtagtg 300

aaatccaatt tcacaacaat gccagtatta caaaatggag gttatatatg ggaaatgatg 360

gaattaacac actgctctca acctaatggc ctaatagatg acaattgtga aattaaattc 420

tccaaaaaac taagtgattc aacaatgacc aattatatga atcaattatc tgaattactt 480

ggatttgacc ttaatccata aattataata aatatcaact agaaaatcaa tgtcactaac 540

accattagtt aatataaaac ttgacagaag ataagaatgg ggcaaataaa tcaactcagc 600

cgacccaacc atggacacaa cacacaatga taccacacca caaagactga tgatcacaga 660

catgagacca ttgtcacttg agactataat aacatcacta accagagaca tcataacaca 720

tagatttata tacttgataa atcatgaatg catagtgaga aaacttgatg aaagacaggc 780

cacatttaca ttcctggtca actatgaaat gaaactattg cacaaagtgg gaagcactaa 840

atataaaaaa tatactgaat acaacacaaa atatggcact ttccctatgc caatatttat 900

caatcatgat gggttcttag aatgcattgg cattaagcct actaagcaca cacccataat 960

atacaagtat gatctcaatc catgaatttt aaaacaagat tcaaacaatc cgaaataaca 1020

acttcatgca taatcacact ccatagtcca aatggagcct gaaaattata gtaatttaaa 1080

attaaggaga gacataagat gaaagatggg gcaaatacaa aaatggctct tagcaaagtc 1140

aagttgaatg atacactcaa caaagatcaa cttctgtcat ccagcaaata caccatccaa 1200

cggagcacag gagacggcat tgacactcct aattatgatg tgcagaaaca catcaataag 1260

ctatgtggca tgttattaat cacagaagat gctaatcata aattcactgg gttaataggt 1320

atgttatatg ctatgtctag attaggaaga gaagacacca taaaaatact caaagatgcg 1380

ggatatcatg ttaaagcaaa tggagtggat gtaacaacac atcgtcaaga cattaatggg 1440

aaagaaatga aatttgaagt gttaacatta gcaagcttaa caactgaaat tcaaatcaac 1500

attgagatag aatctagaaa atcctacaaa aaaatgctaa aagaaatggg agaggtggct 1560

ccagaataca ggcatgactc tcctgattgt gggatgataa tattatgtat agcggcatta 1620

gtaataacca aattagcagc aggggataga tctggtctta cagctgtgat taggagagct 1680

aataatgtcc taaaaaatga aatgaaacgt tataaaggct tactacccaa ggatatagcc 1740

aacagcttct atgaagtgtt tgaaaaatat cctcacttta tagatgtttt tgttcatttt 1800

ggtatagcac aatcttctac cagaggtggc agtagagttg aagggatttt tgcaggattg 1860

tttatgaatg cttatggtgc agggcaagtg atgttacggt ggggggtctt agcaaaatca 1920

gttaaaaata ttatgttagg acacgctagt gtgcaagcag aaatggaaca agttgtggag 1980

gtgtatgaat atgcccaaaa attgggtgga gaagcaggat tctaccatat attgaacaac 2040

ccaaaagcat cattattatc tttgactcaa tttcctcact tctctagtgt agtattaggc 2100

aatgctgctg gcctaggcat aatgggagaa tacagaggta caccaaggaa ccaagatcta 2160

tatgatgctg caaaagcata tgctgaacaa ctcaaagaaa atggtgtgat taactacagt 2220

gtattagact tgacagcaga agagctagag gctatcaaac atcagcttaa tccaaaagat 2280

aatgatgtag agctttgagt taataaaaaa gtggggcaaa taaatcatca tggaaaagtt 2340

tgctcctgaa ttccatggag aagatgcaaa caacagagcc accaaatttc tagaatcaat 2400

aaagggcaaa ttcacatcac ccaaagatcc caagaaaaaa gatagtatca tatctgtcaa 2460

ctctatagat atagaagtaa ccaaagaaag ccctataaca tcaaattcaa ccattataaa 2520

cccaataaat gagacagatg atactgtagg gaacaagccc aattatcaaa gaaaacctct 2580

agtaagtttc aaagaagatc ctacgccaag tgataatccc ttttcaaaac tatacaaaga 2640

aaccatagaa acatttgata acaatgaaga agaatctagc tattcatatg aagaaataaa 2700

tgatcagaca aacgataata taacagcaag attagatagg attgatgaga aattaagtga 2760

aatactggga atgcttcaca cattagtagt agcgagtgca ggacctacat ctgctcggga 2820

tggtataaga gatgccatgg ttggtttaag agaagaaatg atagaaaaaa tcagaactga 2880

agcattaatg accaatgaca gactagaagc tatggcaaga ctcaggaatg aggaaagtga 2940

aaagatggca aaagacacat cagatgaagt gtctctcaat ccaacatcag agaaattgaa 3000

caacctgttg gaagggaatg atagtgacaa tgatctatca cttgaagatt tctgattagc 3060

taccaatctg cacatcaaaa cacaacacca atagaagaac aacaaacaaa ccaactcacc 3120

catccaacca aacatctatc tgccgattag ccaaccagcc aaaaaaacaa ccagctaatc 3180

caaaactagc cacccggata atatcaatac tatagttaca aaaaaagatg gggcaaatat 3240

ggaaacatac gtgaacaaac ttcacgaggg ctccacatac acagctgctg ttcaatacaa 3300

tgtcctagaa aaagacgatg atcctgcatc acttacaata tgggtgccca tgttccaatc 3360

atccatgcca gcagatttac ttataaaaga actagctaat gtcaatatac tagtgaaaca 3420

aatatccaca ctcaagggac cctcattaag agtcatgata aactcaagaa gtgcagtact 3480

agcacaaatg cccagcaaat ttaccatatg tgccaatgtg tccttggatg aaagaagcaa 3540

gctggcatat gatgtaacca caccctgtga aattaaggca tgcagtctaa catgcctaaa 3600

atcagaaaat atgttaacta cagttaaaga tctcactatg aaaacactca acccaacaca 3660

tgacatcatt gctttatgtg aatttgaaaa tatagtaaca tcaaaaaaag tcataatacc 3720

aacataccta agatccatca gtgtcagaaa taaagatctg aacacacttg aaaatataac 3780

aaccactgaa ttcaaaaatg ccattacaaa tgcaaaaatc atcccttact caggattact 3840

gttagtcatc acagtgactg acaacaaagg agcattcaaa tacataaagc cacaaagtca 3900

attcatagta gatcttggag cttacctaga aaaagaaagt atatactatg ttacaacaaa 3960

ttggaagcac acagctacac gatttgcaat taaacccatg gaagattaac cttttctctc 4020

tacatcagtg agttgattca tacaaacttt ctacttacat tcctcaccac acaatcataa 4080

tcaccaactc tctgtggttc aaccaatcaa ataaaattca tcaagagtct cagatcatcc 4140

caaatcattg ttcatcagat ccagtactca aataagttaa taaaaaatcc acatggggca 4200

aataatcatt gagggaaatc caaccaatca caacatctgt caacatagac aagtcaacac 4260

gttagataaa atcaaccaat ggaaaataca tccataacaa tagaattctc aagcaaattc 4320

tggccttact ttacactaat acacatgata acaacaataa tctctttgct aatcataatc 4380

tccatcatga ttgcaatact aaacaaactc tgcgaatata atgtatttca taacaaaacc 4440

tttgagctac caagagcccg agtcaataca tagcattcac caatctgatg gctcaaaaca 4500

gtaaccttgc atttgtaagt gaactaccat cacctcttca caaaaccaca tcaacatctc 4560

accatgcaag ccatcatcta taccataaag tagttaatta aaaatagtca taacaatgaa 4620

ctaagatatt aagaccaaca acaacgttgg ggcaaatgca aacatgtcca aaaccaggga 4680

ccaacgcacc gccaagacac tagaaaggac ctgggacact cttaatcatc tattattcat 4740

atcatcgtgc ttatacaagt taaatcttaa atctatagca caaatcacat tatctatttt 4800

ggcaatgata atctcaactt cacttataat tgcagccatc atattcatag cctcggcaaa 4860

ccacaaagtc acactaacaa ctgcaatcat acaagttgca acaaaccagg tcaagaacac 4920

aaccccaaca tacctcaccc agaatcccca gcttggaatc agcttctcca atctgtccga 4980

aactacatca caacccacca ccatactagc ttcaacaaca ccaagtgctg agtcaacccc 5040

acgatccaca acagtcaaga ccaaaaacac aacaacaacc caaatacaac ctagcaagtc 5100

caccacaaaa caacgccaaa acaaaccaca aaacaaaccc aataatgatt ttcactttga 5160

agtgctcaac tttgtaccct gcagcatatg cagcaacaat ccaacttgct gggctatctg 5220

caaaagaata ccaaacaaaa aacttggaga gaaaaccacc accaagccca caaaaaaacc 5280

aaccaccaag acaaccaaaa aagatctcaa acctcaaacc acaaaaccaa aggaagtact 5340

taccacaaag cccacagaaa agcaaaccat caacaccacc aaaacaaaca tcagaactac 5400

actgctcacc tccaacacca caggaaatcc agaacacaca agtcaaaagg aaaccctcca 5460

ctcaaccacc tccgaaggca atccaagccc ctcacaagtc tatacaacat ccgagtacct 5520

atcacaatct ctatctccat ccaacacaac atactagtag tcattaaaaa gtgtattatt 5580

gcaaaaagcc ataaccaaat caaacagaat caaaatcaac tctggggcaa ataacaatgg 5640

agttgccaat cctcaaagca aatgctatta ccacaatcct tgctgcagtc acactctgtt 5700

ttgcttccag tcaaaacatc actgaagaat tttatcaatc aacatgcagt gcagttagca 5760

aaggctatct tagtgctcta agaactggtt ggtatactag tgttataact atagaattaa 5820

gtaatatcaa ggaaaataag tgtaatggaa cagatgctaa ggtaaaattg ataaaacaag 5880

aattagataa atacaaaaat gctgtaacag aattgcagtt gctcatgcaa agcacaccag 5940

cagccaacaa tcgagccaga agggaattac caagatttat gaattataca ctcaacaata 6000

ctgaaaacac caatgtaaca ttaagcaaga aaaggaaaag aagatttctt ggctttttgt 6060

taggtgttgg atctgcaatc gccagtggca ttgccgtatc caaggtcctg cacctagaag 6120

gggaagtgaa caaaatcaaa agtgctctac tatccacaaa caaggctgta gtcagcttat 6180

caaatggagt cagtgtctta accagcaaag tgttagatct caaaaactat atagataaac 6240

agttgttacc tattgtgaac aagcaaagct gcagcatatc aaacattgaa actgtaatag 6300

agttccaaca aaagaacaac agactactag agattaccag agaatttagt gttaatgcag 6360

gtgtaactac acctgtaagc acttatatgt taactaatag tgaattatta tcattaatca 6420

atgatatgcc tataacaaat gatcagaaaa agttaatgtc caacaatgtt caaatagtta 6480

gacagcaaag ttactctatc atgtccataa taaaagagga agtcttagca tatgtagtac 6540

aattaccact atatggtgta atagatacac cttgttggaa actgcacaca tcccctctat 6600

gtacaaccaa cacaaaggaa gggtccaaca tctgcttaac aagaaccgac agaggatggt 6660

actgtgacaa tgcaggatca gtatctttct tcccacaagc cgaaacatgt aaagttcaat 6720

cgaatcgggt attttgtgac acaatgaaca gtttaacatt accaagtgag gtaagtctct 6780

gcaacgttga catattcaac cccaaatatg attgcaaaat tatgacttca aaaacagatg 6840

taagcagctc cgttatcaca tctctgggag ccattgtgtc atgctatggc aaaaccaaat 6900

gtacagcatc caataaaaat cgtgggatca taaagacatt ttctaacggg tgtgattatg 6960

tatcaaataa gggggtggat actgtgtctg taggtaatac attatattat gtaaataagc 7020

aagaaggcaa aaatctctat gtaaaaggtg aaccaataat aaatttctat gacccattag 7080

tgttcccctc tgatgaattt gatgcatcaa tatctcaagt caatgagaag attaaccaga 7140

gtctagcatt tattcgtaaa tcagatgaat tattacataa tgtaaatgct gttaaatcca 7200

ctacaaatat catgataact actataatta tagtgattat agtaatattg ttatcattaa 7260

ttgtagttgg actgcttcta tactgcaagg ccagaagcac accagtcaca ctaagtaagg 7320

atcaactgag tggtataaat aatattgcat ttagtagctg aataaaatta gcacctaatc 7380

atattcttac aatggttcac tatctgacca tagataaccc atctatcatt ggattttctt 7440

aaaatttgaa cttcatcaca actttcatct ataaaccatc tcacttacac tatttaagta 7500

gattcctatt ttatagttat ataaaacaat tgaataccag attaacttac tatttgtaaa 7560

aataagaact ggggcaaata tgtcacgaag gaatccttgc aaatttgaaa ttcgaggtca 7620

ttgcttgaat ggtaagaggt gtcattttag tcataattat tttgaatggc caccccatgc 7680

actgcttgta agacaaaact ttatgttaaa cagaatactt aagtctatgg ataaaagcat 7740

agatacttta tcagaaataa gtggagctgc agagttggac agaacagaag agtatgccct 7800

cggtgtagtt ggagtgctag aaagttatat aggatcaata aataatataa ctaaacaatc 7860

agcatgtgtt gccatgagca aactcctcac tgaactcaac agtgatgaca tcaaaaaact 7920

aagagacaat gaagagccaa attcacccaa gataagagtg tacaatactg tcatatcata 7980

tattgaaagc aacaggaaaa acaataaaca aactatccat ctgttaaaaa gattgccagc 8040

agacgtattg aagaaaacca tcaaaaacac attggatatc cacaagagca taaccatcaa 8100

taacccaaaa gaatcaactg ttagtgatac gaacgaccat gccaaaaata atgatactac 8160

ctgacaaata tccttgtagt ataaattcca tactaataac aagtagttgt agagttacta 8220

tgtataatca aaaaaacaca ctatatatcg atcaaaacaa ccaaaataac cttatatact 8280

caccgaatca accattcaat gaaatccatt ggacctctca agacttgatt gatgcaactc 8340

aaaattttct acaacatcta ggtattactg atgatatata cacaatatat atattagtgt 8400

cataacactc aatcctaata cttaccacat catcaaatta ttaactcaaa caattcaagc 8460

tatgggacaa aatggatccc attattaatg gaaattctgc taatgtttat ctaactgata 8520

gttatttaaa aggtgttatt tctttctcag aatgtaacgc tttaggaagt tacatactca 8580

atggtcctta tctcaaaaat gattatacca acttaattag tagacaaaat ccattaatag 8640

aacacataaa tctaaaaaaa ctaaatataa cacaatcctt aatatctaag tatcataaag 8700

gtgaaataaa aatagaagaa cctacttatt ttcagtcatt acttatgaca tacaagagta 8760

tgacctcgtc agaacagatt actaccacta atttacttaa aaagataata agaagagcta 8820

tagaaattag tgatgtcaaa gtctatgcta tattgaataa actggggctt aaagagaaag 8880

acaagattaa atccaacaat ggacaagatg aagacaactc agttattaca accataatca 8940

aagatgatat acttttagct gttaaggata atcaatctca tcttaaagca gacaaaaatc 9000

actctacaaa acaaaaagat acaatcaaaa caacactctt gaagaaatta atgtgttcga 9060

tgcaacatcc tccatcatgg ttaatacatt ggtttaattt atacacaaaa ttaaacagca 9120

tattaacaca gtatcgatct agtgaggtaa aaaaccatgg ttttatattg atagataatc 9180

atactcttaa tggattccaa tttattttga atcaatatgg ttgtatagtt tatcataagg 9240

aactcaaaag aattactgtg acaacctata atcaattctt gacatggaaa gatattagcc 9300

ttagtagatt aaatgtttgt ttgattacat ggattagcaa ctgtttgaac acattaaata 9360

aaagcttagg cttaagatgt ggattcaata atgttatctt gacacaatta ttcctttatg 9420

gagattttat actaaaacta tttcacaatg aggggttcta cataataaaa gaggtagagg 9480

gatttattat gtctctaatt ttaaatataa cagaagaaga tcaattcaga aaacggtttt 9540

ataatagtat gctcaacaac atcacagatg ctgctaataa agctcagaaa aatctgctat 9600

caagagtatg tcatacatta ttagataaga cagtatccga taatataata aatggcagat 9660

ggataattct attaagtaag ttccttaaat taattaagct tgcaggtgac aataacctta 9720

acaatctgag tgaattgtat tttttgttca gaatatttgg acacccaatg gtagatgaaa 9780

gacaagccat ggatgctgtt aaagttaatt gcaacgagac caaattttac ttgttaagca 9840

gtttaagtat gttaagaggt gcctttatat atagaattat aaaagggttt gtaaataatt 9900

acaacagatg gcctacttta aggaatgcca ttgttttacc cttaagatgg ttaacttact 9960

ataaactaaa cacttatcct tccttgttgg aacttacaga aagagatttg attgttttat 10020

caggactacg tttttatcga gagtttcggt tgcctaaaaa agtggatctt gaaatgatca 10080

taaatgataa ggctatatca cctcctaaaa atttaatatg gactagtttc cctagaaatt 10140

atatgccgtc acacatacaa aattatatag aacatgaaaa attaaaattc tctgagagtg 10200

ataaatcaag aagagtatta gagtactatt taagagataa caaattcaat gaatgtgatt 10260

tatacaactg tgtagttaat caaagttatc ttaacaaccc taatcatgtg gtatcattga 10320

caggcaaaga aagagaactc agtgtaggta gaatgtttgc aatgcaacca ggaatgttca 10380

gacaagttca aatattagca gaaaaattga tagctgaaaa cattttacaa tttttccctg 10440

aaagtcttac aagatatggt gatctagaac tacagaaaat attagaattg aaagcaggaa 10500

taagtaacaa atcaaatcga tacaatgata attacaacaa ttacattagt aagtgctcta 10560

tcatcacaga tctcagcaaa ttcaatcaag catttcgata tgaaacatca tgtatttgta 10620

gtgatgtact ggatgaactg catggtgtac aatctctatt ttcctggtta catttaacta 10680

ttcctcatgc cacaataata tgcacgtata ggcatgcacc cccctatata agggatcata 10740

ttgtagatct taacaatgta gatgagcaaa gtggattata tagatatcat atgggtggta 10800

tcgaagggtg gtgtcaaaaa ctatggacca tagaagctat atcactatta gatctaatat 10860

ctctcaaagg gaaattctca attactgctt taattaatgg tgacaatcaa tcaatagata 10920

taagtaaacc agtcagactc atggaaggtc aaactcatgc tcaagcagat tatttgctag 10980

cattaaatag tcttaaatta ctgtataaag agtatgcagg cataggccac aaattaaaag 11040

gaactgagac ttatatatca agagatatgc aatttatgag taaaacgatc caacataacg 11100

gtgtatatta cccagctagt ataaagaaag tcctaagagt gggaccgtgg ataaacacta 11160

tacttgatga tttcaaagtg agtctagaat ctataggtag tttgacacag gaattagaat 11220

atagaggtga aagtctatta tgcagtttaa tatttagaaa tgtatggtta tataatcaaa 11280

ttgctttaca actaaaaaat catgcattat gtaacaacaa attatattta gacatattaa 11340

aggttctaaa acacttaaaa acctttttta atcttgataa tattgataca gcattaacat 11400

tgtatatgaa tttgcccatg ttatttggtg gtggtgatcc caacttgtta tatcgaagtt 11460

tctatagaag aactcctgat ttcctcacag aggctatagt tcactctgtg ttcatactta 11520

gttattatac aaaccatgat ttaaaagata aacttcaaga tctgtcagat gatagattga 11580

ataagttctt aacatgcata atcacgtttg acaaaaaccc taatgctgaa ttcgtaacac 11640

tgatgagaga tcctcaagct ttaggatctg agaggcaagc taaaattact agcgaaatca 11700

atagactggc agttactgag gttttgagca cagctccaaa caaaatattc tccaaaagtg 11760

cacaacacta tacaactaca gagatagatc ttaatgatat tatgcaaaat atagaaccta 11820

catatcctca cgggctaaga gttgtttatg aaagtttacc cttttataaa gcagagaaaa 11880

tagtaaatct tatatccggt acaaaatcta taactaacat actggaaaag acttctgcca 11940

tagacttaac agatattgat agagccactg agatgatgag gaaaaacata actttgctta 12000

taaggatatt accattagat tgtaacaaag ataaaagaga aatattgagt atggaaaacc 12060

taagtattac tgaattaagc aaatatgtta gagaaagatc ttggtcttta tctaatatag 12120

ttggtgttac atcacccagt atcatgtata caatggacat caaatataca acaagcacta 12180

tagctagtgg cataatcata gagaaatata atgtcaacag tttaacacgt ggtgagagag 12240

gacccactaa accatgggtt ggttcatcta cacaagagaa aaaaacaatg ccagtttata 12300

atagacaagt tttaaccaaa aaacagagag atcaaataga tctattagca aaattggatt 12360

gggtgtatgc atctatagat aacaaggatg aattcatgga ggaacttagc ataggaactc 12420

ttgggttaac atatgagaaa gccaaaaaat tatttccaca atatttaagt gttaactatt 12480

tgcatcgtct tacagtcagt agtagaccat gtgaattccc tgcatcaata ccagcttata 12540

gaactacaaa ttaccacttt gatactagcc ctattaatcg catattaaca gaaaagtatg 12600

gtgatgaaga tattgatata gtattccaaa actgtataag ctttggcctt agcttaatgt 12660

cagtagtaga acaatttact aatgtatgtc ctaacagaat tattcttata cccaagctta 12720

atgagataca tttgatgaaa ccacctatat tcacaggtga tgttgatatt cacaagttaa 12780

aacaagtgat acaaaaacag catatgtttt taccagacaa aataagtttg actcaatatg 12840

tggaattatt cttaagtaat aaaacactca aatctggatc taatgttaat tctaatttaa 12900

tattggcaca taagatatct gactattttc ataatactta cattttaagt actaatttag 12960

ctgggcattg gattctgatt atacaactta tgaaagattc taagggtatt tttgaaaaag 13020

attggggaga gggatatata actgatcata tgttcattaa tttgaaagtt ttcttcaatg 13080

cttataagac atatctcttg tgttttcata aaggttacgg cagagcaaag ctggaatgtg 13140

atatgaatac ttcagatctc ctatgtgtat tggaattaat agacagtagt tattggaagt 13200

ctatgtctaa ggtattttta gaacaaaaag ttatcaaata cattcttagc caggatgcaa 13260

gtttacatag agtaaaagga tgtcatagct tcaaattatg gttccttaaa cgccttaatg 13320

tagcagaatt cacagtttgc ccttgggttg ttaacataga ttatcatcca acacatatga 13380

aagcaatatt aacttatata gatcttgtta gaatgggatt gataaatata gatagaatat 13440

acattaaaaa taaacacaaa ttcaatgatg aattttatac ttctaatctt ttttacatta 13500

attataactt ctcagataat actcatctat taactaaaca tataaggatt gctaattctg 13560

aattagaaag taattacaac aaattatatc atcctacacc agaaacccta gaaaatatac 13620

taaccaatcc agttaaaagt aatgataaaa agacactaag tgactattgt ataggtaaaa 13680

atgttgactc aataatgtta ccattgttat ctaataagaa gcttattaaa tcgtctacaa 13740

tgattagaac caattacagc agacaagatt tgtataattt atttcctacg gttgtgattg 13800

ataaaattat agatcattca ggtaatacag ccaaatctaa ccaactttac actactactt 13860

ctcatcaaat atctttagtg cacaatagca catcacttta ttgcatgctt ccttggcatc 13920

acattaatag attcaatttt gtatttagtt ctacaggttg taaaattagt atagagtata 13980

ttttaaaaga ccttaaaatt aaagatccta attgtatagc attcataggt gaaggagcag 14040

ggaatttatt attgcgtaca gtagtggaac ttcatcctga tataagatat atttacagaa 14100

gtctgaaaga ttgcaatgat catagtttac caattgagtt tttaaggctg tacaatggac 14160

atatcaacat tgattatggt gaaaatttga ccattcctgc tacagatgca accaacaaca 14220

ttcattggtc ttatttacat ataaagtttg ctgaacctat cagtcttttt gtctgtgatg 14280

ctgaattgcc tgtaacagtc aattggagta aaattataat agagtggagc aagcatgtaa 14340

gaaaatgcaa atattgttct tcagttaata aatgtacgtt aatagtaaaa tatcatgctc 14400

aagatgatat cgatttcaaa ttagacaaca taactatatt aaaaacttat gtatgcttag 14460

gcagtaagtt aaagggatct gaagtttact tagtccttac aataggtcct gcaaatatgt 14520

tccctgtatt tgatgtagta caaaatgcta aattgatact atcaagaacc aaaaatttca 14580

tcatgcctaa gaaggctgat aaagagtcta ttgatgcaaa tattaagagt ttgataccct 14640

ttctttgtta ccctataaca aaaaaaggaa taaatactgc attgtctaaa ctaaagagtg 14700

ttgttagtgg agatatacta tcatattcta tagctggacg caatgaagtt ttcagcaata 14760

aacttataaa tcataagcat atgaacatct taaagtggtt caatcatgtt ttaaatttca 14820

gatcaacaga attaaactat aatcatttat atatggtaga atctacatat cctcatctaa 14880

gtgaattgtt aaacagcttg acaaccaatg aacttaaaaa actgattaaa atcacaggta 14940

gtttgttata caactttcat aatgaataat gaacaaaaat cttataataa aaattcccat 15000

agctacacac taacactgta ttcaattata gttatttaaa attaataatt atataatttt 15060

tttaataact tctagtgaac taatcctaaa attatcattt tgatctagaa ggaataaatt 15120

taaatccaaa tctaattggc ttatatgtat attaactaaa ctacgag 15167

<210> 3

<211> 15004

<212> DNA

<213> Artificial sequence

<400> 3

tggggcaaat aagaatttga taagtaccac ttaaatttaa ctcctttggt tagagatggg 60

cagcaactca ttgagtatga taaaagttag attgcaaaat ttgtttgaca atgatgaagt 120

agcattgtta aaaataacat gctatactga caaattaata cagttaatta atgctttggc 180

taaggcagtt atacatacaa tcaaattgaa tggcattgta tttgtgcatg ttattacaag 240

tagtgatatt tgccctaata ataatatagt agtgaaatcc aatttcacaa caatgccagt 300

attacaaaat ggaggttata tatgggaaat gatggaatta acacactgct ctcaacctaa 360

tggcctaata gatgacaatt gtgaaattaa attctccaaa aaactaagtg attcaacaat 420

gaccaattat atgaatcaat tatctgaatt acttggattt gaccttaatc cataaattat 480

aataaatatc aactagaaaa tcaatgtcac taacaccatt agttaatata aaacttgaca 540

gaagataaga atggggcaaa taaatcaact cagccgaccc aaccatggac acaacacaca 600

atgataccac accacaaaga ctgatgatca cagacatgag accattgtca cttgagacta 660

taataacatc actaaccaga gacatcataa cacatagatt tatatacttg ataaatcatg 720

aatgcatagt gagaaaactt gatgaaagac aggccacatt tacattcctg gtcaactatg 780

aaatgaaact attgcacaaa gtgggaagca ctaaatataa aaaatatact gaatacaaca 840

caaaatatgg cactttccct atgccaatat ttatcaatca tgatgggttc ttagaatgca 900

ttggcattaa gcctactaag cacacaccca taatatacaa gtatgatctc aatccatgaa 960

ttttaaaaca agattcaaac aatccgaaat aacaacttca tgcataatca cactccatag 1020

tccaaatgga gcctgaaaat tatagtaatt taaaattaag gagagacata agatgaaaga 1080

tggggcaaat acaaaaatgg ctcttagcaa agtcaagttg aatgatacac tcaacaaaga 1140

tcaacttctg tcatccagca aatacaccat ccaacggagc acaggagacg gcattgacac 1200

tcctaattat gatgtgcaga aacacatcaa taagctatgt ggcatgttat taatcacaga 1260

agatgctaat cataaattca ctgggttaat aggtatgtta tatgctatgt ctagattagg 1320

aagagaagac accataaaaa tactcaaaga tgcgggatat catgttaaag caaatggagt 1380

ggatgtaaca acacatcatc aagacattaa tgggaaagaa atgaaatttg aagtgttaac 1440

attagcaagc ttaacaactg aaattcaaat caacattgag atagaatcta gaaaatccta 1500

caaaaaaatg ctaaaagaaa tgggagaggt ggctccagaa tacaggcatg actctcctga 1560

ttgtgggatg ataatattat gtatagcggc attagtaata accaaattag cagcagggga 1620

tagatctggt cttacagctg tgattaggag agctaacaat gtcctaaaaa atgaaatgaa 1680

acgttataaa ggcttactac ccaaggatat agccaacagc ttctatgaag tgtttgaaaa 1740

atatcctcac tttatagatg tttttgttca ttttggtata gcacaatctt ctaccagagg 1800

tggcagtaga gttgaaggga tttttgcagg attgtttatg aatgcttatg gtgcagggca 1860

agtgatgtta cggtgggggg tcttagcaaa atcagttaaa aatattatgt taggacacgc 1920

tagtgtgcaa gcagaaatgg aacaagttgt ggaggtgtat gaatatgccc aaaaattggg 1980

tggagaagca ggattctacc atatattgaa caacccaaaa gcatcattat tatctttgac 2040

tcaatttcct cacttctcta gtgtagtatt aggcaatgct gctggcctag gcataatggg 2100

agaatacaga ggtacaccaa ggaaccaaga tctatatgat gctgcaaaag catatgctga 2160

acaactcaaa gaaaatggtg tgattaacta cagtgtatta gacttgacag cagaagagct 2220

agaggctatc aaacatcagc ttaatccaaa agataatgat gtagagcttt gagttaataa 2280

aaaagtgggg caaataaatc atcatggaaa agtttgctcc tgaattccat ggagaagatg 2340

caaacaacag agccaccaaa tttctagaat caataaaggg caaattcaca tcacccaaag 2400

atcccaagaa aaaagatagt atcatatctg tcaactcaat agatatagaa gtaaccaaag 2460

aaagccctat aacatcaaat tcaaccatta taaacccaat aaatgagaca gatgatactg 2520

tagggaacaa gcccaattat caaagaaaac ctctagtaag tttcaaagaa gatcctacgc 2580

caagtgataa tcccttttca aaactataca aagaaaccat agaaacattt gataacaatg 2640

aagaagaatc tagctattca tatgaagaaa taaatgatca gacaaacgat aatataacag 2700

caagattaga taggattgat gagaaattaa gtgaaatact gggaatgctt cacacattag 2760

tagtagcgag tgcaggacct acatctgctc gggatggtat aagagatgcc atggttggtt 2820

taagagaaga aatgatagaa aaaatcagaa ctgaagcatt aatgaccaat gacagactag 2880

aagctatggc aagactcagg aatgaggaaa gtgaaaagat ggcaaaagac acatcagatg 2940

aagtgtctct caatccaaca tcagagaaat tgaacaacct gttggaaggg aatgatagtg 3000

acaatgatct atcacttgaa gatttctgat tagctaccaa tctgcacatc aaaacacaac 3060

accaatagaa gagcaacaaa caaaccaact cacccatcca accaaacatc tatctgccga 3120

ttagccaacc agccaaaaaa acaaccagct aatccaaaac tagccacccg gataatatca 3180

atactatagt tacaaaaaaa gatggggcaa atatggaaac atacgtgaac aaacttcacg 3240

agggctccac atacacagct gctgttcaat acaatgtcct agaaaaagac gatgatcctg 3300

catcacttac aatatgggtg cccatgttcc aatcatccat gccagcagat ttacttataa 3360

aagaactagc taatgtcaat atactagtga aacaaatatc cacactcaag ggaccctcat 3420

taagagtcat gataaactca agaagtgcag tactagcaca aatgcccagc aaatttacca 3480

tatgtgccaa tgtgtccttg gatgaaagaa gcaagctggc atatgatgta accacaccct 3540

gtgaaattaa ggcatgcagt ctaacatgcc taaaatcaaa aaatatgtta actacagtta 3600

aagatctcac tatgaaaaca ctcaacccaa cacatgacat cattgcttta tgtgaatttg 3660

aaaatatagt aacatcaaaa aaagtcataa taccaacata cctaagatcc atcagtgtca 3720

gaaataaaga tctgaacaca cttgaaaata taacaaccac tgaattcaaa aatgccatta 3780

caaatgcaaa aatcatccct tactcaggat tactgttagt catcacagtg actgacaaca 3840

aaggagcatt caaatacata aagccacaaa gtcaattcat agtagatctt ggagcttacc 3900

tagaaaaaga aagtatatac tatgttacaa caaattggaa gcacacagct acacgatttg 3960

caattaaacc catggaagat taaccttttc tctctacatc agtgagttga ttcatacaaa 4020

ctttctactt acattcctca ccacacaatc ataatcacca actctctgtg gttcaaccaa 4080

tcaaataaaa ttcatcaaga gtctcagatc atcccaaatc attgttcatc agatccagta 4140

ctcaaataag ttaataaaaa atccacatgg ggcaaataat cattgaggga aatccaacca 4200

atcacaacat ctgtcaacat agacaagtca acacgttaga caaaatcaac caatggaaaa 4260

tacatccata acaatagaac tctcaagcaa attctggcct tactttacac taatacacat 4320

gataacaaca ataatctctt tgctaatcat aatctccatc atgattgcaa tactaaacaa 4380

actctgcgaa tataatgtat ttcataacaa aacctttgag ctaccaagag cccgagtcaa 4440

tacatagcat tcaccaatct gatggctcaa aacagtaacc ttgcatttgt aagtgaacta 4500

ccatcacctc ttcacaaaac cacatcaaca tctcaccatg caagccatca tctataccat 4560

aaagtagtta attaaaaata gtcataacaa tgaactaaga tattaagacc aacaacaacg 4620

ttggggcaaa tgcaaacatg tccaaaacca aggaccaacg caccgccaag acactagaaa 4680

ggacctggga cactctgaat catctattat tcatatcatc gtgcttatac aagttaaatc 4740

ttaaatctat agcacaaatc acattatcta ttttggcaat gataatctca acttcactta 4800

taattgcagc catcatattc atagcctcgg caaaccacaa agtcacacta acaactgcaa 4860

tcatacaagt tgcaacaaac caggtcaaga acacaacccc aacatacctc acccagaatc 4920

cccagcttgg aatcagcttc tccaatctgt ccgaaactac atcacaaccc accaccatac 4980

tagcttcaac aacaccaagt gctgagtcaa ccccacgatc cacaacagtc aagaccaaaa 5040

acacaacaac aacccaaata caacctagca agtccaccac aaaacaacgc caaaacaaac 5100

cacaaaacaa acccaataat gattttcact ttgaagtgtt caactttgta ccctgcagca 5160

tatgcagcaa caatccaact tgctgggcta tctgcaaaag aataccaaac aaaaaacttg 5220

gagagaaaac caccaccaag cccacaaaaa aaccaaccac caagacaacc aaaaaagatc 5280

tcaaacctca aaccacaaaa ccaaaggaag tacttaccac aaagcccaca gaaaagcaaa 5340

ccatcaacac caccaaaaca aacatcagaa ctacactgct cacctccaac accacaggaa 5400

atccagaaca cacaagtcaa aaggaaaccc tccactcaac cacctccgaa ggcaatccaa 5460

gcccctcaca agtctataca acatccgagt acctatcaca atctctatct ccatccaaca 5520

caacatacta gtagtcatta aaaagtgtat tattgcaaag agccataacc aaatcaaaca 5580

gaatcaaaat caactctggg gcaaataaca atggagttgc caatcctcaa agcaaatgct 5640

attaccacaa tccttgctgc agtcacactc tgttttgctt ccagtcaaaa catcactgaa 5700

gaattttatc aatcaacatg cagtgcagtt agcaaaggct atcttagtgc tctaagaact 5760

ggttggtata ctagtgttat aactatagaa ttaagtaata tcaaggaaaa taagtgtaat 5820

ggaacagatg ctaaggtaaa attgataaaa caagaattag ataaatacaa aaatgctgta 5880

acagaattgc agttgctcat gcaaagcaca ccagcagcca acaatcgagc cagaagggaa 5940

ttaccaagat ttatgaatta tacactcaac aatactgaaa acaccaatgt aacattaagc 6000

aagaaaagga aaagaagatt tcttggcttt ttgttaggtg ttggatctgc aatcgccagt 6060

ggcattgccg tatccaaggt cctgcaccta gaaggggaag tgaacaaaat caaaagtgct 6120

ctactatcca caaacaaggc tgtagtcagc ttatcaaatg gagtcagtgt cttaaccagc 6180

aaagtgttag atctcaaaaa ctatatagat aaacagttgt tacctattgt gaacaagcaa 6240

agctgcagca tatcaaacat tgaaactgta atagagttcc aacaaaagaa caacagacta 6300

ctagagatta ccagagaatt tagtgttaat gcaggtgtaa ctacacctgt aagcacttat 6360

atgttaacta atagtgaatt attatcatta atcaatgata tgcctataac aaatgatcag 6420

aaaaagttaa tgtccaacaa tgttcaaata gttagacagc aaagttactc catcatgtcc 6480

ataataaaag aggaagtctt agcatatgta gtacaattac cactatatgg tgtaatagat 6540

acaccttgtt ggaaactgca cacatcccct ctatgtacaa ccaacacaaa ggaagggtcc 6600

aacatctgct taacaagaac cgacagagga tggtactgtg acaatgcagg atcagtatct 6660

ttcttcccac aagccgaaac atgtaaagtt caatcgaatc gggtattttg tgacacaatg 6720

aacagtttaa cattaccaag tgaggtaagt ctctgcaacg ttgacatatt caaccccaaa 6780

tatgattgca aaattatgac ttcaaaaaca gatgtaagca gctccgttat cacatctctg 6840

ggagccattg tgtcatgcta tggcaaaacc aaatgtacag catccaataa aaatcgtggg 6900

atcataaaga cattttctaa cgggtgtgat tatgtatcaa ataagggggt ggatactgtg 6960

tctgtaggta atacattata ttatgtaaat aagcaagaag gcaaaaatct ctatgtaaaa 7020

ggtgaaccaa taataaattt ctatgaccca ttagtgttcc cctctgatga atttgatgca 7080

tcaatatctc aagtcaatga gaagattaac cagagtctag catttattcg taaatcagat 7140

gaattattac ataatgtaaa tgctgttaaa tccactacaa atatcatgat aactactata 7200

attatagtga ttatagtaat attgttatca ttaattgtag ttggactgct tctatactgc 7260

aaggccagaa gcacaccagt cacactaagt aaggatcaac tgagtggtat aaataatatt 7320

gcatttagta gctgaataaa aatagcacct aatcatattc ttacaatggt tcactatctg 7380

accatagata acccatctat cattggattt tcttaaaatt tgaacttcat cacaactttc 7440

atctataaac catctcactt acactattta agtagattcc tattttatag ttatataaaa 7500

caattgaata ccagattaac ttactatttg taaaaataag aactggggca aatatgtcac 7560

gaaggaatcc ttgcaaattt gaaattcgag gtcattgctt gaatggtaag aggtgtcatt 7620

ttagtcataa ttattttgaa tggccacccc atgcactgct tgtaagacaa aactttatgt 7680

taaacagaat acttaagtct atggataaaa gcatagatac tttatcagaa ataagtggag 7740

ctgcagagtt ggacagaaca gaagagtatg ccctcggtgt agttggagtg ctagaaagtt 7800

atataggatc aataaataat ataactaaac aatcagcatg tgttgccatg agcaaactcc 7860

tcactgaact caacagtgat gacatcaaaa aactaagaga caatgaagag ccaaattcac 7920

ccaagataag attgtacaat actgtcatat catatattga aagcaacagg aaaaacaata 7980

aacaaactat ccatctgtta aaaagattgc cagcagacgt attgaagaaa accatcaaaa 8040

acacattgga tatccacaag agcataacca tcaataaccc aaaagaatca actgttagtg 8100

atacgaacga ccatgccaaa aataatgata ctacctgaca aatatccttg tagtataaat 8160

tccatactaa taacaagtag ttgtagagtt actatgtata atcaaaaaac acactatata 8220

tcgatcaaaa caaccaaaat aaccttatat actcaccgaa tcaaccattc aatgaaatcc 8280

attggacctc tcaagacttg attgatgcaa ctcaaaattt tctacaacat ctaggtatta 8340

ctgatgatat atacacaata tatatattag tgtcataaca ctcaatccta atacttacca 8400

catcatcaaa ttattaactc aaacaattca agctatggga caaaatggat cccattatta 8460

atggaaattc tgctaatgtt tatctaactg atagttattt aaaaggtgtt atttctttct 8520

cagaatgtaa cgctttagga agttacatac tcaatggtcc ttatctcaaa aatgattata 8580

ccaacttaat tagtagacaa aatccattaa tagaacacat aaatctaaaa aaactaaata 8640

taacacaatc cttaatatct aagtatcata aaggtgaaat aaaaatagaa gaacctactt 8700

attttcagtc attacttatg acatacaaga gtatgacctc gtcagaacag attactacca 8760

ctaatttact taaaaagata ataagaagag ctatagaaat tagtgatgtc aaagtctatg 8820

ctatattgaa taaactgggg cttaaagaga aagacaagat taaatccaac aatggacaag 8880

atgaagacaa ctcagttatt acaaccataa tcaaagatga tatactttta gctgttaagg 8940

ataatcaatc tcatcttaaa gcagacaaaa atcactctac aaaacaaaaa gatacaatca 9000

aaacaacact cttgaagaaa ttaatgtgtt cgatgcaaca tcctccatca tggttaatac 9060

attggtttaa tttatacaca aaattaaaca gcatattaac acagtatcga tctagtgagg 9120

taaaaaacca tggttttata ttgatagata agcatactct taatggattc caatttattt 9180

tgaatcaata tggttgtata gtttatcata aggaactcaa aagaattact gtgacaacct 9240

ataatcaatt cttgacatgg aaagatatta gccttagtag attaaatgtt tgtttgatta 9300

catggattag caactgtttg aacacattaa ataaaagctt aggcttaaga tgtggattca 9360

ataatgttat cttgacacaa ttattccttt atggagattt tatactaaaa ctatttcaca 9420

atgaggggtt ctacataata aaagaggtag agggatttat tatgtctcta attttaaata 9480

taacagaaga agatcaattc agaaaacggt tttataatag tatgctcaac aacatcacag 9540

atgctgctaa taaagctcag aaaaatctgc tatcaagagt atgtcataca ttattagata 9600

agacagtatc cgataatata ataaatggca gatggataat tctattaagt aagttcctta 9660

aattaattaa gcttgcaggt gacaataacc ttaacaatct gagtgaattg tatttttgtt 9720

cagaatattt ggacacccaa tggtagatga aagacaagcc atggatgctg ttaaagttaa 9780

ttgcaacgag accaaatttt acttgttaag cagtttaagt atgttaagag gtgcctttat 9840

atatagaatt ataaaagggt ttgtaaataa ttacaacaga tggcctactt taaggaatgc 9900

cattgtttta cccttaagat ggttaactta ctataaacta aacacttatc cttccttgtt 9960

ggaacttaca gaaagagatt tgattgtttt atcaggacta cgtttttatc gagagtttcg 10020

gttgcctaaa aaagtggatc ttgaaatgat cataaatgat aaggctatat cacctcctaa 10080

aaatttaata tggactagtt tccctagaaa ttatatgccg tcacacatac aaaattatat 10140

agaacatgaa aaattaaaat tctctgagag tgataaatca agaagagtat tagagtacta 10200

tttaagagat aacaaattca atgaatgtga tttatacaac tgtgtagtta atcaaagtta 10260

tcttaacaac cctaatcatg tggtatcatt gacaggcaaa gaaagagaac tcagtgtagg 10320

tagaatgttt gcaatgcaac caggaatgtt cagacaagtt caaatattag cagaaaaatt 10380

gatagctgaa aacattttac aatttttccc tgaaagtctt acaagatatg gtgatctaga 10440

actacagaaa atattagaat tgaaagcagg aataagtaac aaatcaaatc gatacaatga 10500

taattacaac aattacatta gtaagtgctc tatcatcaca gatctcagca aattcaatca 10560

agcatttcga tatgaaacat catgtatttg tagtgatgta ctggatgaac tgcatggtgt 10620

acaatctcta ttttcctggt tacatttaac tattcctcat gccacaataa tatgcacgta 10680

taggcatgca cccccctata taagggatca tattgtagat cttaacaatg tagatgagca 10740

aagtggatta tatagatatc atatgggtgg tatcgaaggg tggtgtcaaa aactatggac 10800

catagaagct atatcactat tagatctaat atctctcaaa gggaaattct caattactgc 10860

tttaattaat ggtgacaatc aatcaataga tataagtaaa ccagtcagac tcatggaagg 10920

tcaaactcat gctcaagcag attatttgct agcattaaat agtcttaaat tactgtataa 10980

agagtatgca ggcataggcc acaaattaaa aggaactgag acttatatat caagagatat 11040

gcaatttatg agtaaaacga tccaacataa cggtgtatat tacccagcta gtataaagaa 11100

agtcctaaga gtgggaccgt ggataaacac tatacttgat gatttcaaag tgagtctaga 11160

atctataggt agtttgacac aggaattaga atatagaggt gaaagtctat tatgcagttt 11220

aatatttaga aatgtatggt tatataatca aattgcttta caactaaaaa tcatgcatta 11280

tgtaacaaca aattatattt agacatatta aaggttctaa aacacttaaa aacctttttt 11340

aatcttgata atattgatac agcattaaca ttgtatatga atttgcccat gttatttggt 11400

ggtggtgatc ccaacttgtt atatcgaagt ttctatagaa gaactcctga tttcctcaca 11460

gaggctatag ttcactctgt gttcatactt agttattata caaaccatga tttaaaagat 11520

aaacttcaag atctgtcaga tgatagattg aataagttct taacatgcat aatcacgttt 11580

gacaaaaacc ctaatgctga attcgtaaca ctgatgagag atcctcaagc tttaggatct 11640

gagaggcaag ctaaaattac tagcgaaatc aatagactgg cagttactga ggttttgagc 11700

acagctccaa acaaaatatt ctccaaaagt gcacaacact atacaactac agagatagat 11760

cttaatgata ttatgcaaaa tatagaacct acatatcctc acgggctaag agttgtttat 11820

gaaagtttac ccttttataa agcagagaaa atagtaaatc ttatatccgg tacaaaatct 11880

ataactaaca tactggaaaa gacttctgcc atagacttaa cagatattga tagagccact 11940

gagatgatga ggaaaaacat aactttgctt ataaggatat taccattaga ttgtaacaaa 12000

gataaaagag aaatattgag tatggaaaac ctaagtatta ctgaattaag caaatatgtt 12060

agagaaagat cttggtcttt atctaatata gttggtgtta catcacccag tatcatgtat 12120

acaatggaca tcaaatatac aacaagcact atagctagtg gcataatcat agagaaatat 12180

aatgtcaaca gtttaacacg tggtgagaga ggacccacta aaccatgggt tggttcatct 12240

acacaagaga aaaaaacaat gccagtttat aatagacaag ttttaaccaa aaaacagaga 12300

gatcaaatag atctattagc aaattggatt gggtgtatgc atctatagat aacaaggatg 12360

aattcatgga ggaacttagc ataggaactc ttgggttaac atatgagaaa gccaaaaaat 12420

tatttccaca atatttaagt gttaactatt tgcatcgtct tacagtcagt agtagaccat 12480

gtgaattccc tgcatcaata ccagcttata gaactacaaa ttaccacttt gatactagcc 12540

ctattaatcg catattaaca gaaaagtatg gtgatgaaga tattgatata gtattccaaa 12600

actgtataag ctttggcctt agcttaatgt cagtagtaga acaatttact aatgtatgtc 12660

ctaacagaat tattcttata cccaagctta atgagataca tttgatgaaa ccacctatat 12720

tcacaggtga tgttgatatt cacaagttaa aacaagtgat acaaaaacag catatgtttt 12780

taccagacaa aataagtttg actcaatatg tggaattatt cttaagtaat aaaacactca 12840

aatctggatc taatgttaat tctaatttaa tattggcaca taagatatct gactattttc 12900

ataatactta cattttaagt actaatttag ctgggcattg gattctgatt atacaactta 12960

tgaaagattc taagggtatt tttgaaaaag attggggaga gggatatata actgatcata 13020

tgttcattaa tttgaaagtt ttcttcaatg cttataagac atatctcttg tgttttcata 13080

aaggttacgg cagagcaaag ctggaatgtg atatgaatac ttcagatctc ctatgtgtat 13140

tggaattaat agacagtagt tattggaagt ctatgtctaa ggtattttta gaacaaaaag 13200

ttatcaaata cattcttagc caggatgcaa gtttacatag agtaaaagga tgtcatagct 13260

tcaaattatg gttccttaaa cgccttaatg tagcagaatt cacagtttgc ccttgggttg 13320

ttaacataga ttatcatcca acacatatga aagcaatatt aacttatata gatcttgtta 13380

gaatgggatt gataaatata gatagaatat acattaaaaa taaacacaaa ttcaatgatg 13440

aattttatac ttctaatctt ttttacatta attataactt ctcagataat actcatctat 13500

taactaaaca tataaggatt gctaattctg aattagaaag taattacaac aaattatatc 13560

atcctacacc agaaacccta gaaaatatac taaccaatcc agttaaaagt aatgataaaa 13620

agacactaag tgactattgt ataggtaaaa atgttgactc aataatgtta ccattgttat 13680

ctaataagaa gcttattaaa tcgtctacaa tgattagaac caattacagc agacaagatt 13740

tgtataattt atttcctacg gttgtgattg ataaaattat agatcattca ggtaatacag 13800

ccaaatctaa ccaactttac actactactt ctcatcaaat atctttagtg cacaatagca 13860

catcacttta ttgcatgctt ccttggcatc acattaatag attcaatttt gtatttagtt 13920

ctacaggttg taaaattagt atagagtata ttttaaaaga ccttaaaatt aaagatccta 13980

attgtatagc attcataggt gaaggagcag ggaatttatt attgcgtaca gtagtggaac 14040

ttcatcctga tataagatat atttacagaa gtctgaaaga ttgcaatgat catagtttac 14100

caattgagtt tttaaggctg tacaatggac atatcaacat tgattatggt gaaaatttga 14160

ccattcctgc tacagatgca accaacaaca ttcattggtc ttatttacat ataaagtttg 14220

ctgaacctat cagtcttttt gtctgtgatg ctgaattgcc tgtaacagtc aattggagta 14280

aaattataat agagtggagc aagcatgtaa gaaaatgcaa atattgttct tcagttaata 14340

aatgtacgtt aatagtaaaa tatcatgctc aagatgatat cgatttcaaa ttagacaaca 14400

taactatatt aaaaacttat gtatgcttag gcagtaagtt aaagggatct gaagtttact 14460

tagtccttac aataggtcct gcaaatatgt tccctgtatt tgatgtagta caaaatgcta 14520

aattgatact atcaagaacc aaaaatttca tcatgcctaa gaaggctgat aaagagtcta 14580

ttgatgcaaa tattaagagt ttgataccct ttctttgtta ccctataaca aaaaaaggaa 14640

taaatactgc attgtctaaa ctaaagagtg ttgttagtgg agatatacta tcatattcta 14700

tagctggacg caatgaagtt ttcagcaata aacttataaa tcataagcat atgaacatct 14760

taaagtggtt caatcatgtt ttaaatttca gatcaacaga attaaactat aatcatttat 14820

atatggtaga atctacatat cctcatctaa gtgaattgtt aaacagcttg acaaccaatg 14880

aacttaaaaa actgattaaa atcacaggta gtttgttata caactttcat aatgaataat 14940

gaacaaaaat cttataataa aaattcccat agctacacac taacactgta ttcaattata 15000

gtta 15004

<210> 4

<211> 15093

<212> DNA

<213> Artificial sequence

<400> 4

gggcaaataa gaatttgata agtaccactt aaatttaact cctttggtta gagatgggca 60

gcaactcatt gagtatgata aaagttagat tgcaaaattt gtttgacaat gatgaagtag 120

cattgttaaa aataacatgc tatactgaca aattaataca gttaattaat gctttggcta 180

aggcagttat acatacaatc aaattgaatg gcattgtatt tgtgcatgtt attacaagta 240

gtgatatttg ccctaataat aatatagtag tgaaatccaa tttcacaaca atgccagtat 300

tacaaaatgg aggttatata tgggaaatga tggaattaac acactgctct caacctaatg 360

gcctaataga tgacaattgt gaaattaaat tctccaaaaa actaagtgat tcaacaatga 420

ccaattatat gaatcaatta tctgaattac ttggatttga ccttaatcca taaattataa 480

taaatatcaa ctagaaaatc aatgtcacta acaccattag ttaatataaa acttgacaga 540

agataagaat ggggcaaata aatcaactca gccgacccaa ccatggacac aacacacaat 600

gataccacac cacaaagact gatgatcaca aacatgagac cattgtcact tgagactata 660

ataacatcac taaccagaga catcataaca catagattta tatacttgat aaatcatgaa 720

tgcatagtga gaaaacttga tgaaagacag gccacattta cattcctggt caactatgaa 780

atgaaactat tgcacaaagt gggaagcact aaatataaaa aatatactga atacaacaca 840

aaatatggca ctttccctat gccaatattt atcaatcatg atgggttctt agaatgcatt 900

ggcattaagc ctactaagca cacacccata atatacaagt atgatctcaa tccatgaatt 960

ttaaaacaag attcaaacaa tccgaaataa caacttcatg cataatcaca ctccatagtc 1020

caaatggagc ctgaaaatta tagtaattta aaattaagga gagacataag atgaaagatg 1080

gggcaaatac aaaaatggct cttagcaaag tcaagttgaa tgatacactc aacaaagatc 1140

aacttctgtc atccagcaaa tacaccatcc aacggagcac aggagacggc attgacactc 1200

ctaattatga tgtgcagaaa cacatcaata agctatgtgg catgttatta atcacagaag 1260

atgctaatca taaattcact gggttaatag gtatgttata tgctatgtct agattaggaa 1320

gagaagacac cataaaaata ctcaaagatg cgggatatca tgttaaagca aatggagtgg 1380

atgtaacaac acatcatcaa gacattaatg ggaaagaaat gaaatttgaa gtgttaacat 1440

tagcaagctt aacaactgaa attcaaatca acattgagat agaatctaga aaatcctaca 1500

aaaaaatgct aaaagaaatg ggagaggtgg ctccagaata caggcatgac tctcctgatt 1560

gtgggatgat aatattatgt atagcggcat tagtaataac caaattagca gcaggggata 1620

gatctggtct tacagctgtg attaggagag ctaacaatgt cctaaaaaat gaaatgaaac 1680

gttataaagg cttactaccc aaggatatag ccaacagctt ctatgaagtg tttgaaaaat 1740

atcctcactt tatagatgtt tttgttcatt ttggtatagc acaatcttct accagaggtg 1800

gcagtagagt tgaagggatt tttgcaggat tgtttatgaa tgcttatggt gcagggcaag 1860

tgatgttacg gtggggggtc ttagcaaaat cagttaaaaa tattatgtta ggacacgcta 1920

gtgtgcaagc agaaatggaa caagttgtgg aggtgtatga atatgcccaa aaattgggtg 1980

gagaagcagg attctaccat atattgaaca acccaaaagc atcattatta tctttgactc 2040

aatttcctca cttctctagt gtagtattag gcaatgctgc tggcctaggc ataatgggag 2100

aatacagagg tacaccaagg aaccaagatc tatatgatgc tgcaaaagca tatgctgaac 2160

aactcaaaga aaatggtgtg attaactaca gtgtattaga cttgacagca gaagagctag 2220

aggctatcaa acatcagctt aatccaaaag ataatgatgt agagctttga gttaataaaa 2280

aagtggggca aataaatcat catggaaaag tttgctcctg aattccatgg agaagatgca 2340

aacaacagag ccaccaaatt tctagaatca ataaagggca aattcacatc acccaaagat 2400

cccaagaaaa aagatagtat catatctgtc aactcaatag atatagaagt aaccaaagaa 2460

agccctataa catcaaattc aaccattata aacccaataa atgagacaga tgatactgta 2520

gggaacaagc ccaattatca aagaaaacct ctagtaagtt tcaaagaaga tcctacgcca 2580

agtgataatc ccttttcaaa actatacaaa gaaaccatag aaacatttga taacaatgaa 2640

gaagaatcta gctattcata tgaagaaata aatgatcaga caaacgataa tataacagca 2700

agattagata ggattgatga gaaattaagt gaaatactgg gaatgcttca cacattagta 2760

gtagcgagtg caggacctac atctgctcgg gatggtataa gagatgccat ggttggttta 2820

agagaagaaa tgatagaaaa aatcagaact gaagcattaa tgaccaatga cagactagaa 2880

gctatggcaa gactcaggaa tgaggaaagt gaaaagatgg caaaagacac atcagatgaa 2940

gtgtctctca atccaacatc agagaaattg aacaacctgt tggaagggaa tgatagtgac 3000

aatgatctat cacttgaaga tttctgatta gctaccaatc tgcacatcaa aacacaacac 3060

caatagaaga acaacaaaca aaccaactca cccatccaac caaacatcta tctgccgatt 3120

agccaaccag ccaaaaaaca accagctaat ccaaaactag ccacccggat aatatcaata 3180

ctatagttac aaaaaaagat ggggcaaata tggaaacata cgtgaacaaa cttcacgagg 3240

gctccacata cacagctgct gttcaataca atgtcctaga aaaagacgat gatcctgcat 3300

cacttacaat atgggtgccc atgttccaat catccatgcc agcagattta cttataaaag 3360

aactagctaa tgtcaatata ctagtgaaac aaatatccac actcaaggga ccctcattaa 3420

gagtcatgat aaactcaaga agtgcagtac tagcacaaat gcccagcaaa tttaccatat 3480

gtgccaatgt gtccttggat gaaagaagca agctggcata tgatgtaacc acaccctgtg 3540

aaattaaggc atgcagtcta acatgcctaa aatcaaaaaa tatgttaact acagttaaag 3600

atctcactat gaaaacactc aacccaacac atgacatcat tgctttatgt gaatttgaaa 3660

atatagtaac atcaaaaaaa gtcataatac caacatacct aagatccatc agtgtcagaa 3720

ataaagatct gaacacactt gaaaatataa caaccactga attcaaaaat gccattacaa 3780

atgcaaaaat catcccttac tcaggattac tgttagtcat cacagtgact gacaacaaag 3840

gagcattcaa atacataaag ccacaaagtc aattcatagt agatcttgga gcttacctag 3900

aaaaagaaag tatatactat gttacaacaa attggaagca cacagctaca caatttgcaa 3960

ttaaacccat ggaagattaa ccttttctct ctacatcagt gagttgattc atacaaactt 4020

tctacttaca ttcctcacca cacaatcata atcaccaact ctctgtggtt caaccaatca 4080

aataaaattc atcaagagtc tcagatcatc ccaaatcatt gttcatcaga tccagtactc 4140

aaataagtta ataaaaaatc cacatggggc aaataatcat tgagggaaat ccaaccaatc 4200

acaacatctg tcaacataga caagtcaaca cgttagataa aatcaaccaa tggaaaatac 4260

atccataaca atagaaatct caagcaaatt ctggccttac tttacactaa tacacatgat 4320

aacaacaata atctctttgc taatcataat ctccatcatg attgcaatac taaacaaact 4380

ctgcgaatat aatgtatttc ataacaaaac ctttgagcta ccaagagccc gagtcaatac 4440

atagcattca ccaatctgat ggctcaaaac agtaaccttg catttgtaag tgaactacca 4500

tcacctcttc acaaaaccac atcaacatct caccatgcaa gccatcatct ataccataaa 4560

gtagttaatt aaaaatagtc ataacaatga actaagatat taagaccaac aacaacgttg 4620

gggcaaatgc aaacatgtcc aaaaccaagg accaacgcac cgccaagaca ctagaaagga 4680

cctgggacac tcttaatcat ctattattca tatcatcgtg cttatacaag ttaaatctta 4740

aatctatagc acaaatcaca ttatctattt tggcaatgat aatctcaact tcacttataa 4800

ttgcagccat catattcata gcctcggcaa accacaaagt cacactaaca actgcaatca 4860

tacaagttgc aacaaaccag gtcaagaaca caaccccaac atacctcacc cagaatcccc 4920

agcttggaat cagcttctcc aatctgtccg aaactacatc acaacccacc accatactag 4980

cttcaacaac accaagtgct gagtcaaccc cacgatccac aacagtcaag accaaaaaca 5040

caacaacaac ccaaatacaa cctagcaagt ccaccacaaa acaacgccaa aacaaaccac 5100

aaaacaaacc caataatgat tttcactttg aagtgttcaa ctttgtaccc tgcagcatat 5160

gcagcaacaa tccaacttgc tgggctatct gcaaaagaat accaaacaaa aaacttggag 5220

agaaaaccac caccaagccc acaaaaaaac caaccaccaa gacaaccaaa aaagatctca 5280

aacctcaaac cacaaaacca aaggaagtac ttaccacaaa gcccacagaa aagcaaacca 5340

tcaacaccac caaaacaaac atcagaacta cactgctcac ctccaacacc acaggaaatc 5400

cagaacacac aagtcaaaag gaaaccctcc actcaaccac ctccgaaggc aatccaagcc 5460

cctcacaagt ctatacaaca tccgagtacc tatcacaatc tctatctcca tccaacacaa 5520

catactagta gtcattaaaa agtgtattat tgcaaagagc cataaccaaa tcaaacagaa 5580

tcaaaatcaa ctctggggca aataacaatg gagttgccaa tcctcaaagc aaatgctatt 5640

accacaatcc ttgctgcagt cacactctgt tttgcttcca gtcaaaacat cactgaagaa 5700

ttttatcaat caacatgcag tgcagttagc aaaggctatc ttagtgctct aagaactggt 5760

tggtatacta gtgttataac tatagaatta agtaatatca aggaaaataa gtgtaatgga 5820

acagatgcta aggtaaaatt gataaaacaa gaattagata aatacaaaaa tgctgtaaca 5880

gaattgcagt tgctcatgca aagcacacca gcagccaaca atcgagccag aagggaatta 5940

ccaagattta tgaattatac actcaacaat actgaaaaca ccaatgtaac attaagcaag 6000

aaaaggaaaa gaagatttct tggctttttg ttaggtgttg gatctgcaat cgccagtggc 6060

attgccgtat ccaaggtcct gcacctagaa ggggaagtga acaaaatcaa aagtgctcta 6120

ctatccacaa acaaggctgt agtcagctta tcaaatggag tcagtgtctt aaccagcaaa 6180

gtgttagatc tcaaaaacta tatagataaa cagttgttac ctattgtgaa caagcaaagc 6240

tgcagcatat caaacattga aactgtaata gagttccaac aaaagaacaa cagactacta 6300

gagattacca gagaatttag tgttaatgca ggtgtaacta cacctgtaag cacttatatg 6360

ttaactaata gtgaattatt atcattaatc aatgatatgc ctataacaaa tgatcagaaa 6420

aagttaatgt ccaacaatgt tcaaatagtt agacagcaaa gttactccat catgtccata 6480

ataaaagagg aagtcttagc atatgtagta caattaccac tatatggtgt aatagataca 6540

ccttgttgga aactgcacac atcccctcta tgtacaacca acacaaagga agggtccaac 6600

atctgcttaa caagaaccga cagaggatgg tactgtgaca atgcaggatc agtatctttc 6660

ttcccacaag ccgaaacatg taaagttcaa tcgaatcggg tattttgtga cacaatgaac 6720

agtttaacat taccaagtga ggtaagtctc tgcaacgttg acatattcaa ccccaaatat 6780

gattgcaaaa ttatgacttc aaaaacagat gtaagcagct ccgttatcac atctctggga 6840

gccattgtgt catgctatgg caaaaccaaa tgtacagcat ccaataaaaa tcgtgggatc 6900

ataaagacat tttctaacgg gtgtgattat gtatcaaata agggggtgga tactgtgtct 6960

gtaggtaata cattatatta tgtaaataag caagaaggca aaaatctcta tgtaaaaggt 7020

gaaccaataa taaatttcta tgacccatta gtgttcccct ctgatgaatt tgatgcatca 7080

atatctcaag tcaatgagaa gattaaccag agtctagcat ttattcgtaa atcagatgaa 7140

ttattacata atgtaaatgc tgttaaatcc actacaaata tcatgataac tactataatt 7200

atagtgatta tagtaatatt gttatcatta attgtagttg gactgcttct atactgcaag 7260

gccagaagca caccagtcac actaagtaag gatcaactga gtggtataaa taatattgca 7320

tttagtagct gaataaaaat agcacctaac catattctta caatggttca ctatctgacc 7380

atagataacc catctatcat tggattttct taaaatttga acttcatcac aactttcatc 7440

tataaaccat ctcacttaca ctatttaagt agattcctat tttatagtta tataaaacaa 7500

ttgaatacca gattaactta ctatttgtaa aaataagaac tggggcaaat atgtcacgaa 7560

ggaatccttg caaatttgaa attcgaggtc attgcttgaa tggtaagagg tgtcatttta 7620

gtcataatta ttttgaatgg ccaccccatg cactgcttgt aagacaaaac tttatgttaa 7680

acagaatact taagtctatg gataaaagca tagatacttt atcagaaata agtggagctg 7740

cagagttgga cagaacagaa gagtatgccc tcggtgtagt tggagtgcta gaaagttata 7800

taggatcaat aaataatata actaaacaat cagcatgtgt tgccatgagc aaactcctca 7860

ctgaactcaa cagtgatgac atcaaaaaac taagagacaa tgaagagcca aattcaccca 7920

agataagagt gtacaatact gtcatatcat atattgaaag caacaggaaa aacaataaac 7980

aaactatcca tctgttaaaa agattgccag cagacgtatt gaagaaaacc atcaaaaaca 8040

cattggatat ccacaagagc ataaccatca ataacccaaa agaatcaact gttagtgata 8100

cgaacgacca tgccaaaaat aatgatacta cctgacaaat atccttgtag tataaattcc 8160

atactaataa caagtagttg tagagttact atgtataatc aaaaaaacac actatatatc 8220

gatcaaaaca accaaaataa ccttatatac tcaccgaatc aaccattcaa tgaaatccat 8280

tggacctctc aagacttgat tgatgcaact caaaattttc tacaacatct aggtattact 8340

gatgatatat acacaatata tatattagtg tcataacact caatcctaat acttaccaca 8400

tcatcaaatt attaactcaa acaattcaag ctatgggaca aaatggatcc cattattaat 8460

ggaaattctg ctaatgttta tctaactgat agttatttaa aaggtgttat ttctttctca 8520

gaatgtaacg ctttaggaag ttacatactc aatggtcctt atctcaaaaa tgattatacc 8580

aacttaatta gtagacaaaa tccattaata gaacacataa atctaaaaaa actaaatata 8640

acacaatcct taatatctaa gtatcataaa ggtgaaataa aaatagaaga acctacttat 8700

tttcagtcat tacttatgac atacaagagt atgacctcgt cagaacagat tactaccact 8760

aatttactta aaaagataat aagaagagct atagaaatta gtgatgtcaa agtctatgct 8820

atattgaata aactggggct taaagagaaa gacaagatta aatccaacaa tggacaagat 8880

gaagacaact cagttattac aaccataatc aaagatgata tacttttagc tgttaaggat 8940

aatcaatctc atcttaaagc agacaaaaat cactctacaa aacaaaaaga tacaatcaaa 9000

acaacactct tgaagaaatt aatgtgttcg atgcaacatc ctccatcatg gttaatacat 9060

tggtttaatt tatacacaaa attaaacagc atattaacac agtatcgatc tagtgaggta 9120

aaaaaccatg gttttatatt gatagataag catactctta atggattcca atttattttg 9180

aatcaatatg gttgtatagt ttatcataag gaactcaaaa gaattactgt gacaacctat 9240

aatcaattct tgacatggaa agatattagc cttagtagat taaatgtttg tttgattaca 9300

tggattagca actgtttgaa cacattaaat aaaagcttag gcttaagatg tggattcaat 9360

aatgttatct tgacacaatt attcctttat ggagatttta tactaaaact atttcacaat 9420

gaggggttct acataataaa agaggtagag ggatttatta tgtctctaat tttaaatata 9480

acagaagaag atcaattcag aaaacggttt tataatagta tgctcaacaa catcacagat 9540

gctgctaata aagctcagaa aaatctgcta tcaagagtat gtcatacatt attagataag 9600

acagtatccg ataatataat aaatggcaga tggataattc tattaagtaa gttccttaaa 9660

ttaattaagc ttgcaggtga caataacctt aacaatctga gtgaattgta ttttttgttc 9720

agaatatttg gacacccaat ggtagatgaa agacaagcca tggatgctgt taaagttaat 9780

tgcaacgaga ccaaatttta cttgttaagc agtttaagta tgttaagagg tgcctttata 9840

tatagaatta taaaagggtt tgtaaataat tacaacagat ggcctacttt aaggaatgcc 9900

attgttttac ccttaagatg gttaacttac tataaactaa acacttatcc ttccttgttg 9960

gaacttacag aaagagattt gattgtttta tcaggactac gtttttatcg agagtttcgg 10020

ttgcctaaaa aagtggatct tgaaatgatc ataaatgata aggctatatc acctcctaaa 10080

aatttaatat ggactagttt ccctagaaat tatatgccgt cacacataca aaattatata 10140

gaacatgaaa aattaaaatt ctctgagagt gataaatcaa gaagagtatt agagtactat 10200

ttaagagata acaaattcaa tgaatgtgat ttatacaact gtgtagttaa tcaaagttat 10260

cttaacaacc ctaatcatgt ggtatcattg acaggcaaag aaagagaact cagtgtaggt 10320

agaatgtttg caatgcaacc aggaatgttc agacaagttc aaatattagc agaaaaattg 10380

atagctgaaa acattttaca atttttccct gaaagtctta caagatatgg tgatctagaa 10440

ctacagaaaa tattagaatt gaaagcagga ataagtaaca aatcaaatcg atacaatgat 10500

aattacaaca attacattag taagtgctct atcatcacag atctcagcaa attcaatcaa 10560

gcatttcgat atgaaacatc atgtatttgt agtgatgtac tggatgaact gcatggtgta 10620

caatctctat tttcctggtt acatttaact attcctcatg ccacaataat atgcacgtat 10680

aggcatgcac ccccctatat aagggatcat attgtagatc ttaacaatgt agatgagcaa 10740

agtggattat atagatatca tatgggtggt atcgaagggt ggtgtcaaaa actatggacc 10800

atagaagcta tatcactatt agatctaata tctctcaaag ggaaattctc aattactgct 10860

ttaattaatg gtgacaatca atcaatagat ataagtaaac cagtcagact catggaaggt 10920

caaactcatg ctcaagcaga ttatttgcta gcattaaata gtcttaaatt actgtataaa 10980

gagtatgcag gcataggcca caaattaaaa ggaactgaga cttatatatc aagagatatg 11040

caatttatga gtaaaacgat ccaacataac ggtgtatatt acccagctag tataaagaaa 11100

gtcctaagag tgggaccgtg gataaacact atacttgatg atttcaaagt gagtctagaa 11160

tctataggta gtttgacaca ggaattagaa tatagaggtg aaagtctatt atgcagttta 11220

atatttagaa atgtatggtt atataatcaa attgctttac aactaaaaaa tcatgcatta 11280

tgtaacaaca aattatattt agacatatta aaggttctaa aacacttaaa aacctttttt 11340

aatcttgata atattgatac agcattaaca ttgtatatga atttgcccat gttatttggt 11400

ggtggtgatc ccaacttgtt atatcgaagt ttctatagaa gaactcctga tttcctcaca 11460

gaggctatag ttcactctgt gttcatactt agttattata caaaccatga tttaaaagat 11520

aaacttcaag atctgtcaga tgatagattg aataagttct taacatgcat aatcacgttt 11580

gacaaaaacc ctaatgctga attcgtaaca ctgatgagag atcctcaagc tttaggatct 11640

gagaggcaag ctaaaattac tagcgaaatc aatagactgg cagttactga ggttttgagc 11700

acagctccaa acaaaatatt ctccaaaagt gcacaacact atacaactac agagatagat 11760

cttaatgata ttatgcaaaa tatagaacct acatatcctc acgggctaag agttgtttat 11820

gaaagtttac ccttttataa agcagagaaa atagtaaatc ttatatccgg tacaaaatct 11880

ataactaaca tactggaaaa gacttctgcc atagacttaa cagatattga tagagccact 11940

gagatgatga ggaaaaacat aactttgctt ataaggatat taccattaga ttgtaacaaa 12000

gataaaagag aaatattgag tatggaaaac ctaagtatta ctgaattaag caaatatgtt 12060

agagaaagat cttggtcttt atctaatata gttggtgtta catcacccag tatcatgtat 12120

acaatggaca tcaaatatac aacaagcact atagctagtg gcataatcat agagaaatat 12180

aatgtcaaca gtttaacacg tggtgagaga ggacccacta aaccatgggt tggttcatct 12240

acacaagaga aaaaaacaat gccagtttat aatagacaag ttttaaccaa aaaacagaga 12300

gatcaaatag atctattagc aaaattggat tgggtgtatg catctataga taacaaggat 12360

gaattcatgg aggaacttag cataggaact cttgggttaa catatgagaa agccaaaaaa 12420

ttatttccac aatatttaag tgttaactat ttgcatcgtc ttacagtcag tagtagacca 12480

tgtgaattcc ctgcatcaat accagcttat agaactacaa attaccactt tgatactagc 12540

cctattaatc gcatattaac agaaaagtat ggtgatgaag atattgatat agtattccaa 12600

aactgtataa gctttggcct tagcttaatg tcagtagtag aacaatttac taatgtatgt 12660

cctaacagaa ttattcttat acccaagctt aatgagatac atttgatgaa accacctata 12720

ttcacaggtg atgttgatat tcacaagtta aaacaagtga tacaaaaaca gcatatgttt 12780

ttaccagaca aaataagttt gactcaatat gtggaattat tcttaagtaa taaaacactc 12840

aaatctggat ctaatgttaa ttctaattta atattggcac ataagatatc tgactatttt 12900

cataatactt acattttaag tactaattta gctgggcatt ggattctgat tatacaactt 12960

atgaaagatt ctaagggtat ttttgaaaaa gattggggag agggatatat aactgatcat 13020

atgttcatta atttgaaagt tttcttcaat gcttataaga catatctctt gtgttttcat 13080

aaaggttacg gcagagcaaa gctggaatgt gatatgaata cttcagatct cctatgtgta 13140

ttggaattaa tagacagtag ttattggaag tctatgtcta aggtattttt agaacaaaaa 13200

gttatcaaat acattcttag ccaggatgca agtttacata gagtaaaagg atgtcatagc 13260

ttcaaattat ggttccttaa acgccttaat gtagcagaat tcacagtttg cccttgggtt 13320

gttaacatag attatcatcc aacacatatg aaagcaatat taacttatat agatcttgtt 13380

agaatgggat tgataaatat agatagaata tacattaaaa ataaacacaa attcaatgat 13440

gaattttata cttctaatct tttttacatt aattataact tctcagataa tactcatcta 13500

ttaactaaac atataaggat tgctaattct gaattagaaa gtaattacaa caaattatat 13560

catcctacac cagaaaccct agaaaatata ctaaccaatc cagttaaaag taatgataaa 13620

aagacactaa gtgactattg tataggtaaa aatgttgact caataatgtt accattgtta 13680

tctaataaga agcttattaa atcgtctaca atgattagaa ccaattacag cagacaagat 13740

ttgtataatt tatttcctac ggttgtgatt gataaaatta tagatcattc aggtaataca 13800

gccaaatcta accaacttta cactactact tctcatcaaa tatctttagt gcacaatagc 13860

acatcacttt attgcatgct tccttggcat cacattaata gattcaattt tgtatttagt 13920

tctacaggtt gtaaaattag tatagagtat attttaaaag accttaaaat taaagatcct 13980

aattgtatag cattcatagg tgaaggagca gggaatttat tattgcgtac agtagtggaa 14040

cttcatcctg atataagata tatttacaga agtctgaaag attgcaatga tcatagttta 14100

ccaattgagt ttttaaggct gtacaatgga catatcaaca ttgattatgg tgaaaatttg 14160

accattcctg ctacagatgc aaccaacaac attcattggt cttatttaca tataaagttt 14220

gctgaaccta tcagtctttt tgtctgtgat gctgaattgc ctgtaacagt caattggagt 14280

aaaattataa tagagtggag caagcatgta agaaaatgca aatattgttc ttcagttaat 14340

aaatgtacgt taatagtaaa atatcatgct caagatgata tcgatttcaa attagacaac 14400

ataactatat taaaaactta tgtatgctta ggcagtaagt taaagggatc tgaagtttac 14460

ttagtcctta caataggtcc tgcaaatatg ttccctgtat ttgatgtagt acaaaatgct 14520

aaattgatac tatcaagaac caaaaatttc atcatgccta agaaggctga taaagagtct 14580

attgatgcaa atattaagag tttgataccc tttctttgtt accctataac aaaaaaagga 14640

ataaatactg cattgtctaa actaaagagt gttgttagtg gagatatact atcatattct 14700

atagctggac gcaatgaagt tttcagcaat aaacttataa atcataagca tatgaacatc 14760

ttaaagtggt tcaatcatgt tttaaatttc agatcaacag aattaaacta taatcattta 14820

tatatggtag aatctacata tcctcatcta agtgaattgt taaacagctt gacaaccaat 14880

gaacttaaaa aactgattaa aatcacaggt agtttgttat acaactttca taatgaataa 14940

tgaacaaaaa tcttataata aaaattccca tagctacaca ctaacactgt attcaattat 15000

agttatttaa aattaataat tatataattt ttttaataac ttctagtgaa ctaatcctaa 15060

aattatcatt ttgatctaga aggaataaat tta 15093

Claims (12)

1. A human respiratory syncytial virus strain, which is a subtype A strain, and is selected from at least one of the following strains: strain 1, strain 2, strain 3 and strain 4;

the preservation number of the strain 1 is CCTCC NO: v201746; the preservation number of the strain 2 is CCTCC NO: v201755; the preservation number of the strain 3 is CCTCC NO: v202033; the preservation number of the strain 4 is CCTCC NO: v202034.

2. Use of the human respiratory syncytial virus strain of claim 1 in the preparation of an attenuated strain of human respiratory syncytial virus.

3. Use of the human respiratory syncytial virus strain of claim 1 in the manufacture of a medicament for the treatment or prevention of respiratory syncytial virus infection.

4. The use of claim 3, wherein the medicament is a vaccine.

5. The use according to claim 4, wherein the medicament is a live attenuated vaccine or a protein component vaccine.

6. A hybridoma cell produced by immunizing an animal with the human respiratory syncytial virus strain of claim 1 as an immunogen.

7. The hybridoma cell according to claim 6, wherein the animal is a mouse.

8. The hybridoma cell according to claim 7, wherein the mouse is a BALB/c mouse.

9. An antiserum produced by immunizing an animal with the human respiratory syncytial virus strain of claim 1 as an immunogen.

10. The antiserum according to claim 9, wherein said animal is a mouse, monkey, rabbit or sheep.

11. An antibody produced by immunizing an animal with the human respiratory syncytial virus strain of claim 1 as an immunogen.

12. The antibody of claim 11, wherein the animal is a mouse, monkey, rabbit, or sheep.

Images