CN112501019A - Multifunctional bioreactor - Google Patents

Multifunctional bioreactor Download PDF

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CN112501019A
CN112501019A CN202011394956.6A CN202011394956A CN112501019A CN 112501019 A CN112501019 A CN 112501019A CN 202011394956 A CN202011394956 A CN 202011394956A CN 112501019 A CN112501019 A CN 112501019A
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arm
culture
module
cylinder
fixing seat
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CN112501019B (en
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钟华强
张传杰
袁玉宇
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Medprin Regenerative Medical Technologies Co Ltd
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Medprin Regenerative Medical Technologies Co Ltd
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Abstract

The invention provides a multifunctional bioreactor. The utility model provides a multifunctional bioreactor, wherein, includes the main casing body, locates be used for on the inside bottom surface of main casing body circulating culture device who cultivates to the culture, locate be used for snatching on the inside top surface of main casing body the tissue tongs of culture among the circulating culture device locates on the inside top surface of main casing body and the ring locate tissue tongs first bionical arm module all around, the bionical arm module of second, electro photoluminescence module and growth factor supplement module, and locate environment regulation and control module on the inside lateral wall of main casing body. The invention can carry out diversified culture on large tissues or complete organs, and perfect the final functions of the large tissues or the organs.

Description

Multifunctional bioreactor
Technical Field
The invention relates to the technical field of tissue engineering, in particular to a multifunctional bioreactor.
Background
In 4 months of 2019, a scientific research team in Israel prints a first global complete heart by using human tissues taken from patients through a 3D printing technology, wherein the heart comprises cells, blood vessels, a heart cavity and a heart chamber, is equivalent to a rabbit heart in size, cannot pump blood at present and only can contract, and needs to be cultured in a culture device in a later stage for further experiments to perfect the heart function.
The existing cell culture devices and bioreactors are mostly prepared for two-dimensional culture of common cells or culture of specific small-sized tissues, such as stem cells, muscle tissues, blood vessels and the like, and specific culture means, such as addition of certain growth factors, certain mechanical stimulation and the like, are also needed; however, if the whole organ is cultured, the culture means to be considered needs to be more diversified, the culture period is longer, and some problems may occur in the culture process and need to be adjusted in time, so that it is necessary to develop a bioreactor which has diversified culture means, can adjust the functions flexibly in the culture process, and does not need to change the structure greatly.
Disclosure of Invention
In order to overcome the problems that most of the existing cell culture devices and bioreactors are prepared for two-dimensional culture of common cells or culture of tissues with specific small sizes and cannot be suitable for culture of a complete organ, the invention provides a multifunctional bioreactor. The invention can carry out diversified culture on large tissues or complete organs, and perfect the final functions of the large tissues or the organs.
In order to solve the technical problems, the invention adopts the technical scheme that: the utility model provides a multifunctional bioreactor, wherein, includes the main casing body, locates be used for on the inside bottom surface of main casing body circulating culture device who cultivates to the culture, locate be used for snatching on the inside top surface of main casing body the tissue tongs of culture among the circulating culture device locates on the inside top surface of main casing body and the ring locate tissue tongs first bionical arm module all around, the bionical arm module of second, electro photoluminescence module and growth factor supplement module, and locate environment regulation and control module on the inside lateral wall of main casing body. The first bionic arm module and the second bionic arm module can perform different mechanical stimulations on the culture grabbed by the tissue grabber, can temporarily fix the culture grabbed by the tissue grabber, and can also be used for removing hyperplastic tissues on the culture; the electrical stimulation module can electrically stimulate the culture; the growth factor supplementation module may be positioned to supplement some growth factors as needed during the culturing process. The environment regulation and control module provides a proper culture environment for the culture by optimizing the air in the main shell and regulating and controlling the temperature in the main shell. According to the invention, the culture function is modularized by the arrangement of the circulating culture device, the first bionic arm module, the second bionic arm module, the electrical stimulation module and the growth factor supplement module, a plurality of culture functions are integrated, and a certain module can be flexibly replaced to realize a new culture function, so that a bioreactor does not need to be greatly improved or redeveloped, new culture requirements can be realized by only adjusting a part of structure, further more diversified culture experiments can be carried out, and the final large-scale tissue or organ functions are perfected.
Furthermore, the first bionic arm module and the second bionic arm module are symmetrically arranged on two opposite sides of the tissue gripper, and the electrical stimulation module and the growth factor supplement module are symmetrically arranged on the other two opposite sides of the tissue gripper.
Further, organize the tongs including locating electric putter on the main casing body top lateral surface, one end through first connecting block with electric putter connects the other end and passes through preformed hole on the main casing body top lateral wall penetrates the inside slide bar of main casing body, through the second connecting block with the revolving cylinder that the slide bar other end is connected to and connect in through the third connecting block the last tongs of revolving cylinder. The slide bar can slide from top to bottom along with electric putter's motion, and revolving cylinder can drive the tongs and carry out 360 rotations to satisfy the cultivation demand of the different positions of culture, and the tongs can carry out the centre gripping to the culture, then slides along with the slide bar and picks up whole culture from circulation culture apparatus, then coordinates other functional module and carries out some operations.
Furthermore, the first bionic arm module comprises a first fixed seat fixedly connected to the inner wall of the top of the main shell, a first arm with one end connected with the first fixed seat through a first speed reduction motor, a second arm with one end connected with the other end of the first arm through a second speed reduction motor, a micro cylinder with a cylinder barrel end connected with the other end of the second arm through a third speed reduction motor, a finger fixed seat for fixing the micro cylinder on the end face of one end of the finger fixed seat through the cylinder fixed seat, and fingers symmetrically arranged on two sides of the other end of the finger fixed seat; a piston rod of the micro cylinder is connected with one end of the finger through a pull rod and can drive the finger to clamp or loosen; the structure of the second bionic arm module is the same as that of the first bionic arm module. The first arm and the second arm are arms simulating human beings, can swing up and down through the rotation of the first speed reducing motor and the second speed reducing motor, and the micro cylinder can swing along with the rotation of the third speed reducing motor to simulate the swing of human wrists.
Furthermore, the electric stimulation module includes a second fixing seat fixedly connected to the inner wall of the top of the main casing body, a third arm with one end connected to the second fixing seat through a fourth speed reduction motor, a fourth arm with one end connected to the other end of the third arm through a fifth speed reduction motor, an electric needle fixing seat connected to the other end of the fourth arm through a sixth speed reduction motor, and an electric needle arranged on the electric needle fixing seat, wherein the electric needle can electrically stimulate the culture.
Furthermore, the growth factor supplementing module comprises a third fixed seat fixedly connected to the inner wall of the top of the main shell, a fifth arm with one end connected with the third fixed seat through a seventh speed reducing motor, a sixth arm with one end connected with the other end of the fifth arm through an eighth speed reducing motor, a needle cylinder fixed seat connected with the other end of the sixth arm through a ninth speed reducing motor, and a needle cylinder arranged on the needle cylinder fixed seat; the one end that the cylinder is close to the cylinder fixing base is equipped with the cover, the cylinder is kept away from the one end of cylinder fixing base is equipped with some glue syringe needles, and growth factor extrudes through some glue syringe needles. The needle cylinder fixing seat is provided with a through hole for a pipeline, one end of the pipeline is connected to the cylinder cover, and the other end of the pipeline is connected with external equipment for providing production factors.
Further, circulation culture apparatus includes to be fixed in through the support frame the cultivation pond under the tissue tongs, locate cultivate the stock solution bottle of pond one side and locate the peristaltic pump of support frame below, cultivation pond, stock solution bottle and peristaltic pump form circulation circuit through hose end to end connection in proper order. Thus, the culture can be dynamically cultured in the culture pond, and the culture medium can be renewed.
Furthermore, the bottom surface of the culture pond is a gradient surface which gradually decreases from the periphery to the middle, so that the culture can be ensured to be kept at the middle position of the culture pond, and the tissue gripper can conveniently grip the culture.
Furthermore, at least one lateral wall of culture pond and main casing body is made by transparent material, conveniently observes the culture of culture pond inside.
Further, the environment control module comprises a belt filtering air inlet arranged on one inner side wall of the main shell, a temperature controller arranged on the other inner side wall of the main shell and a PTC heater arranged on the bottom surface inside the main shell. The air inlet with the filter can filter and purify air entering the main shell, and provides an aerobic environment for the inside of the main shell. The temperature controller and the PTC heater can be matched to control the temperature in the main shell, so that a proper temperature environment is provided for the culture process.
Compared with the prior art, the invention has the following beneficial effects:
the invention realizes the collection of a plurality of culture functions by simultaneously arranging the circulating culture device, the first bionic arm module, the second bionic arm module, the electrical stimulation module and the growth factor supplement module in one bioreactor, and can provide dynamic culture, mechanical stimulation, electrical stimulation and positioning supplement of growth factors for cultures. In addition, the invention carries out modularized treatment on the culture function, which is beneficial to flexibly replacing or adding the function of a certain module in the culture process so as to meet the new culture requirement, and further can carry out diversified culture on large tissues or complete organs so as to perfect the final functions of the large tissues or organs.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
FIG. 2 is a schematic view of the tissue gripper of the present invention.
FIG. 3 is a schematic structural diagram of the first/second bionic arm modules of the present invention.
Fig. 4 is a schematic structural diagram of an electrical stimulation module according to the present invention.
FIG. 5 is a schematic diagram of the structure of a growth factor supplementation module according to the present invention.
FIG. 6 is a schematic view showing the internal structure of a culture tank of the present invention.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and are not to be construed as limiting the present patent.
As shown in fig. 1, a multifunctional bioreactor, which comprises a main housing 1, a circular culture device arranged on the bottom surface inside the main housing 1 for circular culture of a culture, a tissue gripper 2 arranged on the top surface inside the main housing 1 for gripping the culture in the circular culture device, a first bionic arm module 3, a second bionic arm module 4, an electrical stimulation module 5 and a growth factor supplement module 6 arranged on the top surface inside the main housing 1 and around the tissue gripper 2, and an environment regulation and control module arranged on the side wall inside the main housing 1. The first bionic arm module 3 and the second bionic arm module 4 can perform different mechanical stimulations on the culture grabbed by the tissue grabber 2, can temporarily fix the culture grabbed by the tissue grabber 2, and can also be used for removing hyperplastic tissues on the culture; the electrical stimulation module 5 can electrically stimulate the culture; growth factor supplementation module 6 may be positioned to supplement some growth factors as needed during the culturing process. The environment regulating module provides a proper culture environment for the culture by optimizing the air in the main housing 1 and regulating the temperature in the main housing 1. According to the invention, the culture function is modularized by the arrangement of the circulating culture device, the first bionic arm module 3, the second bionic arm module 4, the electrical stimulation module 5 and the growth factor supplement module 6, a plurality of culture functions are integrated, and a certain module can be flexibly replaced to realize a new culture function, so that a bioreactor does not need to be greatly improved or redeveloped, new culture requirements can be realized by only adjusting a part of structure, further diversified culture experiments can be carried out, and the final functions of large tissues or organs are perfected.
As shown in fig. 1, the first and second bionic arm modules 3 and 4 are symmetrically disposed on two opposite sides of the tissue gripper 2, and the electrical stimulation module 5 and the growth factor supplement module 6 are symmetrically disposed on the other two opposite sides of the tissue gripper 2.
As shown in fig. 2, the tissue gripper 2 includes an electric push rod 201 disposed on the outer side surface of the top of the main housing 1, a slide bar 203 having one end connected to the electric push rod 201 via a first connecting block 202 and the other end penetrating into the main housing 1 via a preformed hole on the side wall of the top of the main housing 1, a rotary cylinder 205 connected to the other end of the slide bar 203 via a second connecting block 204, and a gripper 207 connected to the rotary cylinder 205 via a third connecting block 206. The sliding bar 203 can slide up and down along with the movement of the electric push rod 201, the rotating cylinder 205 can drive the gripper 207 to rotate for 360 degrees so as to meet the culture requirements of different parts of the culture, the gripper 207 can grip the culture, then the whole culture is gripped from the circulating culture device along with the sliding of the sliding bar 203, and then the operation is carried out by matching with other functional modules.
As shown in fig. 3, the first bionic arm module 3 includes a first fixing base 301 fixedly connected to the inner wall of the top of the main housing 1, a first arm 303 having one end connected to the first fixing base 301 through a first speed reduction motor 302, a second arm 305 having one end connected to the other end of the first arm 303 through a second speed reduction motor 304, a micro cylinder 307 having a cylinder end connected to the other end of the second arm 305 through a third speed reduction motor 306, a finger fixing base 309 fixing the micro cylinder 307 on the end surface of one end thereof through a cylinder fixing base 308, and fingers 3010 symmetrically disposed at two sides of the other end of the finger fixing base 309; a piston rod of the micro cylinder 307 is connected with one end of the finger 3010 through a pull rod 3011 and can drive the finger 3010 to clamp or loosen; the structure of the second bionic arm module 4 is the same as that of the first bionic arm module 3. The first arm 303 and the second arm 305 are arms simulating a human, and can swing up and down by the rotation of the first reduction motor 302 and the second reduction motor 304, and the micro cylinder 307 can swing along with the rotation of the third reduction motor 306, simulating the swing of a human wrist.
As shown in fig. 4, the electrical stimulation module 5 includes a second fixing base 501 fixedly connected to the inner wall of the top of the main housing 1, a third arm 503 having one end connected to the second fixing base 501 through a fourth gear motor 502, a fourth arm 505 having one end connected to the other end of the third arm 503 through a fifth gear motor 504, an electrical needle fixing base 507 connected to the other end of the fourth arm 505 through a sixth gear motor 506, and an electrical needle 508 disposed on the electrical needle fixing base 507, wherein the electrical needle 508 can electrically stimulate the culture.
As shown in fig. 5, the growth factor supplementation module 6 includes a third fixing seat 601 fixedly connected to the inner wall of the top of the main housing 1, a fifth arm 603 having one end connected to the third fixing seat 601 through a seventh speed-reducing motor 602, a sixth arm 605 having one end connected to the other end of the fifth arm 603 through an eighth speed-reducing motor 604, a syringe fixing seat 607 connected to the other end of the sixth arm 605 through a ninth speed-reducing motor 606, and a syringe 608 disposed on the syringe fixing seat 607; a syringe cover 609 is arranged at one end of the syringe 608 close to the syringe fixing seat 607, a dispensing needle 6010 is arranged at one end of the syringe 608 far from the syringe fixing seat 607, and growth factors are extruded out through the dispensing needle 6010. A through hole 6011 is formed in the syringe fixing seat 607, through which a pipeline, one end of which is connected to the syringe cover 609 and the other end of which is connected to an external device for providing production factors, passes.
As shown in fig. 1, the circulating culture apparatus includes a culture tank 8 fixed under the tissue gripper 2 by a support frame 7, a liquid storage bottle 9 disposed on one side of the culture tank 8, and a peristaltic pump 10 disposed under the support frame 7, wherein the culture tank 8, the liquid storage bottle 9, and the peristaltic pump 10 are sequentially connected end to end by a hose 11 to form a circulating loop. Thus, the culture can be dynamically cultured in the culture tank 8 and the medium can be renewed.
As shown in FIG. 6, the bottom surface of the culture pond 8 is a gradient surface 801 gradually decreasing from the periphery to the middle, so that the culture can be kept at the middle position of the culture pond 8, and the tissue gripper 2 can grasp the culture conveniently.
In this embodiment, the culture pond 8 and at least one side wall of the main housing 1 are made of transparent materials, so that the culture inside the culture pond 8 can be observed conveniently.
As shown in fig. 1, the environment control module includes a filtered air inlet 12 disposed on one inner sidewall of the main housing 1, a temperature controller 13 disposed on the other inner sidewall of the main housing 1, and a PTC heater 14 disposed on the bottom surface of the main housing 1. The filtered air inlet 12 may filter and purify air entering the interior of the main housing 1 to provide an aerobic environment to the interior of the main housing 1. The temperature controller 13 and the PTC heater 14 can cooperate to control the temperature inside the main housing 1, so as to provide a proper temperature environment for the culture process.
The working process of the embodiment is as follows: firstly, the temperature controller 13 is started, the PTC heater 14 starts to heat, the air inlet 12 with the filtering air inlet starts to feed air, the interior of the shell is heated to a proper temperature and a proper oxygen environment, the liquid storage bottle 9 is filled with a culture medium, the culture tissue is placed in the culture pond 8, the peristaltic pump 10 is started, and the culture medium flows through the hose 11 and enters the culture pond 8 to start circulating culture. Taking a heart capable of contracting as an example mentioned in the background art, after a period of time of culture, the contraction frequencies of different positions of heart tissues are found to be different, and after preliminary analysis, it is presumed that the printing material is not uniform in the printing process, which results in a large cell density at the left atrium position and a small cell density at the right atrium position, so that the contraction frequency at the left atrium position is fast, and the contraction frequency at the right atrium position is slow. The method comprises the steps that an electric push rod 201 moves downwards, a slide bar 203 drives a hand grip 207 to move downwards to grip heart tissues, after the gripping is finished, the electric push rod 201 moves upwards, the slide bar 203 drives the hand grip 207 to move upwards to grip the heart tissues to the upper side of a culture pond 8, a fourth speed reducing motor 502 rotates anticlockwise to enable a third arm 503 to be close to the heart tissues, a fifth speed reducing motor 504 rotates clockwise to enable the third arm 503 and a fourth arm 505 to be collinear, a sixth speed reducing motor 506 rotates clockwise to drive an electric needle 508 to come to the position of the left atrium and slowly contact the outer surface of the left atrium, the electric needle 508 inputs a gentle electrocardio frequency to reduce the contraction frequency of the left atrium, and meanwhile, a fifth arm 603 and a sixth arm 605 of a growth factor supplementing module 6 cooperatively move through a seventh speed reducing motor 602, an eighth speed reducing motor 604 and a ninth speed reducing motor 606 to enable a needle 6010 to come inside a dispensing chamber, the syringe 608 is loaded with some cardiac cell mixed liquid with growth factors in advance, the pipeline connected with the cover of the syringe 608 is ventilated, so that a part of the cardiac cell mixed liquid with the growth factors is injected into the right atrium, after the injection is finished, the eighth reducing motor 604 rotates to withdraw the dispensing needle 6010, the rotary cylinder 205 is ventilated to start rotating, the gripper 207 drives the cardiac tissue to rotate, the surface of the right atrium comes near the electrical stimulation module 5, and meanwhile, the electric needle 508 comes to the position of the right atrium and slowly contacts the outer surface of the right atrium, and the electric needle 508 inputs a gentle cardiac electrical frequency, so that the contraction frequency of the right atrium is increased and is consistent with the contraction frequency of the left atrium. If the hand grip 207 blocks certain parts of the heart tissue, the electric stimulation module 5 and the growth factor supplement module 6 cannot be operated, the first bionic arm module 3 and the second bionic arm module 4 come near the heart tissue, the micro cylinder 307 is ventilated to drive the pull rod 3011, so that the two fingers 3010 clamp the heart tissue and temporarily fix the heart tissue, the rotary cylinder 205 drives the hand grip 207 to adjust the angle, the heart tissue is grabbed again, and then the operations are performed.
After culturing for a period of time, if some hyperplastic tissues appear in the heart tissue, the operation of the tissue gripper 2 can be repeated, the first bionic arm module 3 comes near the heart tissue, the two fingers 3010 extend into the heart tissue, the micro cylinder 307 is ventilated to drive the pull rod 3011, the two fingers 3010 clamp the hyperplastic tissues and pull the hyperplastic tissues outwards to remove the hyperplastic tissues, and in addition, the two fingers 3010 can also be used for clamping and stimulating the heart tissue to simulate a mechanical experiment.
In addition, because the culture time of the complete organ is very long, results beyond some experimental designs or new culture requirements may appear in the culture process, the culture function is modularized, the function of a certain module can be flexibly replaced or added to meet the new culture requirements, and thus, the bioreactor does not need to be greatly improved or redeveloped, the new culture requirements can be realized only by adjusting a certain part of structure, further more diversified culture experiments can be carried out, and the functions of the final large tissues or organs are perfected.
It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. The utility model provides a multifunctional bioreactor, its characterized in that, includes main casing body (1), locates be used for on the inside bottom surface of main casing body (1) circulating culture device who cultivates that circulates, locate be used for grabbing on the inside top surface of main casing body (1) tissue tongs (2) of the culture among the circulating culture device locate on the inside top surface of main casing body (1) and the ring locate tissue tongs (2) first bionical arm module (3), the bionical arm module of second (4), electro photoluminescence module (5) and growth factor supplement module (6) all around, and locate environment regulation and control module on the inside lateral wall of main casing body (1).
2. The multifunctional bioreactor according to claim 1, wherein the first and second bionic arm modules (3, 4) are symmetrically disposed on opposite sides of the tissue gripper (2), and the electrical stimulation module (5) and the growth factor supplement module (6) are symmetrically disposed on the other opposite sides of the tissue gripper (2).
3. The bioreactor according to claim 1 or 2, wherein the tissue gripper (2) comprises an electric push rod (201) arranged on the outer side surface of the top of the main casing (1), a slide bar (203) with one end connected with the electric push rod (201) through a first connecting block (202) and the other end penetrating into the main casing (1) through a preformed hole on the side wall of the top of the main casing (1), a rotary cylinder (205) connected with the other end of the slide bar (203) through a second connecting block (204), and a gripper (207) connected with the rotary cylinder (205) through a third connecting block (206).
4. The multifunctional bioreactor according to claim 1 or 2, wherein the first bionic arm module (3) comprises a first fixing seat (301) fixedly connected to the inner wall of the top of the main shell (1), a first arm (303) with one end connected to the first fixing seat (301) through a first speed reduction motor (302), a second arm (305) with one end connected to the other end of the first arm (303) through a second speed reduction motor (304), a micro cylinder (307) with a cylinder end connected to the other end of the second arm (305) through a third speed reduction motor (306), a finger fixing seat (309) for fixing the micro cylinder (307) on the end face of one end thereof through a cylinder fixing seat (308), and fingers (3010) symmetrically arranged at two sides of the other end of the finger fixing seat (309); a piston rod of the micro cylinder (307) is connected with one end of the finger (3010) through a pull rod (3011) and can drive the finger (3010) to clamp or loosen; the structure of the second bionic arm module (4) is the same as that of the first bionic arm module (3).
5. The multifunctional bioreactor according to claim 1 or 2, wherein the electrical stimulation module (5) comprises a second fixing base (501) fixedly connected to the inner wall of the top of the main housing (1), a third arm (503) having one end connected to the second fixing base (501) through a fourth gear motor (502), a fourth arm (505) having one end connected to the other end of the third arm (503) through a fifth gear motor (504), an electrical needle fixing base (507) connected to the other end of the fourth arm (505) through a sixth gear motor (506), and an electrical needle (508) disposed on the electrical needle fixing base (507).
6. The multifunctional bioreactor according to claim 1 or 2, wherein the growth factor supplement module (6) comprises a third fixing seat (601) fixedly connected to the inner wall of the top of the main casing (1), a fifth arm (603) with one end connected to the third fixing seat (601) through a seventh speed reduction motor (602), a sixth arm (605) with one end connected to the other end of the fifth arm (603) through an eighth speed reduction motor (604), a syringe fixing seat (607) connected to the other end of the sixth arm (605) through a ninth speed reduction motor (606), and a syringe (608) arranged on the syringe fixing seat (607); a cylinder cover (609) is arranged at one end, close to the cylinder fixing seat (607), of the cylinder (608), a dispensing needle (6010) is arranged at one end, far away from the cylinder fixing seat (607), of the cylinder (608), and a through hole (6011) through which a pipeline, one end of which is connected to the cylinder cover (609) and the other end of which is connected with outside equipment for providing production factors, passes, is formed in the cylinder fixing seat (607).
7. The multifunctional bioreactor according to claim 1 or 2, characterized in that the circulating culture device comprises a culture tank (8) fixed under the tissue gripper (2) by a support frame (7), a liquid storage bottle (9) arranged on one side of the culture tank (8), and a peristaltic pump (10) arranged under the support frame (7), wherein the culture tank (8), the liquid storage bottle (9) and the peristaltic pump (10) are sequentially connected end to end by a hose (11) to form a circulating loop.
8. The multifunctional bioreactor according to claim 7, wherein the bottom surface of the culture tank (8) is a gradient surface (801) gradually decreasing from the periphery to the middle.
9. Multifunctional bioreactor according to claim 7, characterized in that the culture tank (8) and at least one side wall of the main housing (1) are made of transparent material.
10. The multifunctional bioreactor according to claim 1 or 2, wherein the environment control module comprises a filtered air inlet (12) provided on one inner side wall of the main housing (1), a temperature controller (13) provided on the other inner side wall of the main housing (1), and a PTC heater (14) provided on the inner bottom surface of the main housing (1).
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