CN112470924A - Orchidaceae plant culture medium and preparation method thereof - Google Patents
Orchidaceae plant culture medium and preparation method thereof Download PDFInfo
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- CN112470924A CN112470924A CN202011379771.8A CN202011379771A CN112470924A CN 112470924 A CN112470924 A CN 112470924A CN 202011379771 A CN202011379771 A CN 202011379771A CN 112470924 A CN112470924 A CN 112470924A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
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Abstract
The invention provides an orchid culture medium and a preparation method thereof, and relates to the technical field of plant tissue culture. An orchid culture medium, which comprises the following components: banana, potato, peptone, activated carbon, white sugar, agar and nutrient mixture and water. The culture medium is adjusted on the formula aiming at the orchids, provides nutrition for the tissues of the orchids by using other common raw materials to enable the orchids to grow and reproduce, and has good effect and convenient use. In addition, the invention also provides a preparation method of the orchid culture medium, which comprises the following steps: preparing nutrient solution, processing materials, mixing raw materials and sterilizing. The method has simple preparation process, and the prepared culture medium has good culture effect.
Description
Technical Field
The invention relates to the technical field of plant tissue culture, in particular to an orchid culture medium and a preparation method thereof.
Background
The cymbidium plant is the first major family of all plants worldwide, the first major family in monocotyledonous plants. There is a long history of cultivation and numerous varieties. In nature, a plurality of wild orchids with ornamental value are yet to be developed, protected and utilized. Mainly comprises Chinese traditional orchid, Chinese traditional wild orchid and series orchid varieties.
In tissue culture of orchids, a culture medium is needed for tissue propagation, and a commonly used culture medium on the market at present is difficult to generate an excellent culture effect when being used for tissue culture of orchids, and the configuration process of the culture medium is complicated and inconvenient to use.
Disclosure of Invention
The invention aims to provide an orchid culture medium, which is adjusted according to the formula of an orchid plant, provides nutrition for the tissue of the orchid plant by using other common raw materials so as to enable the tissue of the orchid plant to grow and reproduce, and is good in effect and convenient to use.
The invention also aims to provide a preparation method of the orchid culture medium, which has simple preparation process and better culture effect of the prepared culture medium.
The technical problem to be solved by the invention is realized by adopting the following technical scheme:
in one aspect, the embodiment of the present invention provides an orchid culture medium, which comprises the following components: banana, potato, peptone, activated carbon, white sugar, agar and nutrient mixture and water.
Further, in some embodiments of the present invention, the components in parts by weight are: 2-80 parts of banana, 2-100 parts of potato, 1-20 parts of peptone, 0.5-10 parts of activated carbon, 1-100 parts of white sugar, 1-10 parts of agar, 1.6-6.2 parts of nutrient and 800 parts of water-activated sugar.
Further, in some embodiments of the present invention, the nutrient mixture comprises the following components: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride.
Further, in some embodiments of the present invention, the nutrient mixture comprises the following components in parts by weight: 0.5-2.5 parts of potassium nitrate, 0.5-2 parts of ammonium nitrate, 0.1-0.3 part of monopotassium phosphate, 0.2-0.6 part of magnesium sulfate and 0.3-0.8 part of calcium chloride.
Further, in some embodiments of the present invention, the water is deionized water.
On the other hand, the embodiment of the invention also provides a preparation method of the orchid culture medium, which comprises the following steps:
preparing a nutrient solution: adding water into the nutrient mixture, and then mixing the nutrient mixture by ultrasonic to obtain a nutrient solution for later use;
material treatment: peeling potatoes, cleaning the potatoes under ultrasonic waves, cutting the potatoes into blocks, cooking the blocks, putting the cooked potatoes and peeled bananas into a wall breaking machine, crushing and stirring the potatoes and the peeled bananas into mud for later use;
mixing raw materials: adding peptone, activated carbon, white sugar and agar into the nutrient solution, adding mashed potato and banana, heating while stirring, mixing, and placing into a culture bottle;
and (3) sterilization treatment: and (4) placing the culture bottle into a sterilization pot for sterilization, placing the culture bottle into a sterilization cabinet or a sterilization cooling workshop for cooling after the sterilization is finished, and obtaining a finished product after the cooling is finished.
Further, in some embodiments of the present invention, the above-mentioned nutrient solution is ultrasonically mixed for 15-30min at a frequency of 28KHZ during the preparation process.
Further, in some embodiments of the present invention, the above material treatment process comprises cutting potato into pieces and cooking at 80-95 deg.C for 15-30 min.
Further, in some embodiments of the present invention, the above raw materials are mixed while heating to 80-95 ℃ under stirring.
Further, in some embodiments of the present invention, during the sterilization process, the culture bottle is placed in a sterilization pot and sterilized at 120-.
Compared with the prior art, the embodiment of the invention has at least the following advantages or beneficial effects:
in view of the first aspect, the embodiment of the present invention provides an orchid culture medium, which includes the following components: banana, potato, peptone, activated carbon, white sugar, agar and nutrient mixture and water.
In the above examples, the potato is rich in nutrients such as carbohydrates, plant proteins, vitamin C and dietary fibers, the banana is rich in nutrients such as vitamin a, phosphorus, thiamine, riboflavin, potassium and magnesium, both of which contain plant hormones, and these substances can provide nutrients required by the orchid for tissue culture, thus being beneficial to the growth and propagation of the orchid; the peptone is rich in organic nitrogen compounds, vitamins and saccharides, contains rich amino acids, particularly more sulfur-containing amino acids, can provide a nitrogen source for tissue culture of orchidaceae plants, and is beneficial to growth and reproduction of plant tissues; the activated carbon has adsorption effect and can adsorb some harmful impurities, thereby reducing the influence of the harmful impurities on the growth of the tissue of the orchid and being beneficial to the growth and the propagation of the tissue of the orchid; the white sugar is used as a carbon source, can provide energy for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The agar can be used as a coagulator, which is beneficial to the use of a culture medium; the nutrient mixture contains various nutrient elements, can provide sufficient nutrition for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The orchid culture medium composed of the components is adjusted on the formula of the orchid plant, and other common raw materials are used for providing nutrition for tissues of the orchid plant to enable the orchid plant to grow and reproduce, so that the orchid culture medium is good in effect and convenient to use.
In view of the second aspect, the embodiment of the present invention further provides a preparation method of an orchid culture medium, which includes the following steps: preparing nutrient solution, processing materials, mixing raw materials and sterilizing.
In the embodiment, firstly, the nutrient solution is prepared through the nutrient solution preparation step, water is added into the nutrient mixture, and the nutrient mixture and the water are mixed through ultrasound, so that the nutrient mixture and the water can be more fully mixed, and the obtained nutrient solution is favorable for improving the effect of nutrients on tissue culture of orchids; then, the potatoes and the bananas are processed through the material processing steps, the potatoes are peeled and cut into blocks for cooking, the cutting into blocks can reduce the time required for cooking the potatoes, and some toxic substances in the potatoes can be removed through cooking, so that the growth and the propagation of orchid tissues are facilitated, meanwhile, the texture of the potatoes can be softened, the potatoes and the peeled bananas are conveniently put into a wall breaking machine together for crushing and stirring into mud in the subsequent steps, and the use is more convenient; through the raw material mixing step, peptone, activated carbon, white sugar and agar are added into the nutrient solution, and mashed potatoes and bananas are added, and the nutrient solution, the peptone, the activated carbon, the white sugar, the agar, the mashed potatoes and the bananas can be mixed more fully through heating and stirring, so that the mutual combination and the synergistic effect of the components are facilitated, the culture effect of the orchid plant tissue is improved, and the orchid plant tissue is more convenient to use; finally, through the sterilization treatment steps, the culture bottle is placed in a sterilization pot for sterilization, and is placed in a sterilization cabinet or a sterilization cooling workshop for cooling after sterilization is completed, so that harmful strains and impurities in the culture bottle are removed, the toxicity of the harmful strains and the impurities is reduced, and the tissue of the orchid can be cultured, so that the culture effect of the tissue of the orchid is improved, the growth and the propagation of the tissue of the orchid are facilitated, and the use is more convenient. The whole preparation method has simple flow, the prepared culture medium has good culture effect, is beneficial to the growth and the propagation of the tissue of the orchids, and is convenient to use.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present invention will be described in detail below with reference to specific examples.
The embodiment of the invention provides an orchid culture medium, which comprises the following components: banana, potato, peptone, activated carbon, white sugar, agar and nutrient mixture and water.
In the above examples, the potato is rich in nutrients such as carbohydrates, vegetable proteins, vitamin C, dietary fibers, etc., and the banana is rich in nutrients such as vitamin a, phosphorus, thiamine, riboflavin, potassium, magnesium, etc., and these nutrients can provide nutrients required by the orchid for tissue culture, thus being beneficial to growth and propagation of the orchid; the peptone is rich in organic nitrogen compounds, vitamins and saccharides, contains rich amino acids, particularly more sulfur-containing amino acids, can provide a nitrogen source for tissue culture of orchidaceae plants, and is beneficial to growth and reproduction of plant tissues; the activated carbon has adsorption effect and can adsorb some harmful impurities, thereby reducing the influence of the harmful impurities on the growth of the tissue of the orchid and being beneficial to the growth and the propagation of the tissue of the orchid; the white sugar is used as a carbon source, can provide energy for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The agar can be used as a coagulator, which is beneficial to the use of a culture medium; the nutrient mixture contains various nutrient elements, can provide sufficient nutrition for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The orchid culture medium composed of the components is adjusted on the formula of the orchid plant, and other common raw materials are used for providing nutrition for tissues of the orchid plant to enable the orchid plant to grow and reproduce, so that the orchid culture medium is good in effect and convenient to use.
Further, in some embodiments of the present invention, the components in parts by weight are: 2-80 parts of banana, 2-100 parts of potato, 1-20 parts of peptone, 0.5-10 parts of activated carbon, 1-100 parts of white sugar, 1-10 parts of agar, 1.6-6.2 parts of nutrient and 800 parts of water-activated sugar.
In the embodiment, the components are favorably combined and synergized by controlling the weight parts of the components, so that the culture effect of the culture medium on the tissue of the orchid plant is further improved, the growth and the propagation of the tissue of the orchid plant are favorably realized, the culture effect cannot be influenced due to improper parts, and the use is more convenient.
Further, in some embodiments of the present invention, the nutrient mixture comprises the following components: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride.
In the above embodiment, potassium nitrate can provide nitrogen and potassium, ammonium nitrate can also provide nitrogen, potassium dihydrogen phosphate contains phosphorus and potassium, magnesium sulfate can provide water-soluble magnesium and sulfur to the tissue of the orchid plant, calcium chloride can provide calcium, and can play a role in assisting agar coagulation, and the components can provide nutrient elements required by the tissue culture of the orchid plant, so that the growth and reproduction of the tissue of the orchid plant are facilitated.
Further, in some embodiments of the present invention, the nutrient mixture comprises the following components in parts by weight: 0.5-2.5 parts of potassium nitrate, 0.5-2 parts of ammonium nitrate, 0.1-0.3 part of monopotassium phosphate, 0.2-0.6 part of magnesium sulfate and 0.3-0.8 part of calcium chloride.
In the embodiment, by controlling the weight parts of the components of the nutrient mixture, the beneficial elements in the components can fully exert the effects, so that the culture effect on the tissue of the orchid plant can be further improved, the growth and the propagation of the tissue of the orchid plant can be further facilitated, and the culture effect cannot be influenced due to improper mixture ratio.
Further, in some embodiments of the present invention, the water is deionized water.
In the above embodiment, deionized water is adopted, which is beneficial to further improving the culture effect of the culture medium on the tissue of the orchid plant, so that the influence of impurities brought by water on the growth and propagation of the tissue of the orchid plant is avoided, and the quality of the culture medium is further improved.
The embodiment of the invention also provides a preparation method of the orchid culture medium, which comprises the following steps:
preparing a nutrient solution: adding water into the nutrient mixture, and then mixing the nutrient mixture by ultrasonic to obtain a nutrient solution for later use;
material treatment: peeling potatoes, cleaning the potatoes under ultrasonic waves, cutting the potatoes into blocks, cooking the blocks, putting the cooked potatoes and peeled bananas into a wall breaking machine, crushing and stirring the potatoes and the peeled bananas into mud for later use;
mixing raw materials: adding peptone, activated carbon, white sugar and agar into the nutrient solution, adding mashed potato and banana, heating while stirring, mixing, and placing into a culture bottle;
and (3) sterilization treatment: and (4) placing the culture bottle into a sterilization pot for sterilization, placing the culture bottle into a sterilization cabinet or a sterilization cooling workshop for cooling after the sterilization is finished, and obtaining a finished product after the cooling is finished.
In the embodiment, firstly, the nutrient solution is prepared through the nutrient solution preparation step, water is added into the nutrient mixture, and the nutrient mixture and the water are mixed through ultrasound, so that the nutrient mixture and the water can be more fully mixed, and the obtained nutrient solution is favorable for improving the effect of nutrients on tissue culture of orchids; then, the potatoes and the bananas are processed through the material processing steps, the potatoes are peeled and cut into blocks for cooking, the cutting into blocks can reduce the time required for cooking the potatoes, and some toxic substances in the potatoes can be removed through cooking, so that the growth and the propagation of orchid tissues are facilitated, meanwhile, the texture of the potatoes can be softened, the potatoes and the peeled bananas are conveniently put into a wall breaking machine together for crushing and stirring into mud in the subsequent steps, and the use is more convenient; through the raw material mixing step, peptone, activated carbon, white sugar and agar are added into the nutrient solution, and mashed potatoes and bananas are added, and the nutrient solution, the peptone, the activated carbon, the white sugar, the agar, the mashed potatoes and the bananas can be mixed more fully through heating and stirring, so that the mutual combination and the synergistic effect of the components are facilitated, the culture effect of the orchid plant tissue is improved, and the orchid plant tissue is more convenient to use; finally, through the sterilization treatment steps, the culture bottle is placed into a sterilization pot for sterilization, and is placed into a disinfection cabinet or a disinfection cooling workshop for cooling after the sterilization is finished, so that harmful strains and impurities in the culture bottle are removed, the toxicity of the harmful strains and the impurities is reduced, the culture effect on the orchid tissue is improved, the growth and the propagation of the orchid tissue are facilitated, and the use is more convenient. The whole preparation method has simple flow, the prepared culture medium has good culture effect, is beneficial to the growth and the propagation of the tissue of the orchids, and is convenient to use.
Further, in some embodiments of the present invention, the above-mentioned nutrient solution is ultrasonically mixed for 15-30min at a frequency of 28KHZ during the preparation process.
In the above embodiment, by controlling the frequency and the ultrasonic time of the ultrasonic, the nutrient mixture and the water can be mixed more sufficiently, and beneficial elements cannot be damaged due to improper ultrasonic frequency or duration, so that the using effect of the culture medium is influenced, the quality of the culture medium is further improved, and the growth and the propagation of the orchid tissue are facilitated.
Further, in some embodiments of the present invention, the above material treatment process comprises cutting potato into pieces and cooking at 80-95 deg.C for 15-30 min.
In the embodiment, the cooking temperature and time are controlled, so that toxic substances in the potatoes can be better removed, meanwhile, the texture of the potatoes is softened through cooking, the potatoes can be more conveniently crushed and stirred into mud, and the potato-shredding-and-baking-type potato-and-baking machine is more convenient to use.
Further, in some embodiments of the present invention, the above raw materials are mixed while heating to 80-95 ℃ under stirring.
In the above-mentioned embodiment, through control heating temperature, be favorable to combining the better stirring of each component together, make each component full play beneficial component, and can not make beneficial component suffer destruction because of the temperature is uncomfortable, influence the cultivation effect to further promote the quality of culture medium, further improve the cultivation effect to the orchid tissue, it is more convenient to use.
Further, in some embodiments of the present invention, during the sterilization process, the culture bottle is placed in a sterilization pot and sterilized at 120-.
In the above embodiment, the temperature and time of sterilization and disinfection are controlled, so that the toxic and harmful strains and impurities are completely eliminated, the culture medium components cannot be damaged due to improper temperature or time, the culture effect of the culture medium is not affected, the quality of the culture medium is further improved through sterilization and disinfection, the growth and the propagation of orchid tissues are facilitated, and the use is more convenient.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
This example provides an orchid culture medium prepared by the following steps:
preparing a nutrient solution: adding 800g of water into a nutrient mixture consisting of 0.5g of potassium nitrate, 0.5g of ammonium nitrate, 0.1g of monopotassium phosphate, 0.2g of magnesium sulfate and 0.3g of calcium chloride, and ultrasonically mixing for 15min at the frequency of 28KHZ to obtain a nutrient solution for later use;
material treatment: peeling 30g of potato, cleaning under ultrasonic, cutting into pieces, steaming at 80 deg.C for 15min, peeling the cooked potato and 20g of banana, pulverizing in a wall breaking machine, and stirring to obtain paste;
mixing raw materials: adding 1g of peptone, 0.5g of activated carbon, 1g of white sugar and 1g of agar into the nutrient solution, adding mashed potato and banana, heating to 80 ℃, stirring, mixing, and placing into a culture bottle after stirring;
and (3) sterilization treatment: placing the culture bottle into a sterilizing pot, sterilizing at 120 deg.C for 15min, placing into a sterilizing cabinet or sterilizing cooling workshop, cooling, and getting the final product.
Example 2
This example provides an orchid culture medium prepared by the following steps:
preparing a nutrient solution: adding 1000g of water into a nutrient mixture consisting of 2.5g of potassium nitrate, 2g of ammonium nitrate, 0.3g of monopotassium phosphate, 0.6g of magnesium sulfate and 0.8g of calcium chloride, and then ultrasonically mixing for 30min at the frequency of 28KHZ to obtain a nutrient solution for later use;
material treatment: peeling 100g of potato, cleaning under ultrasonic, cutting into pieces, steaming at 95 deg.C for 30min, peeling the cooked potato and 80g of banana, pulverizing in a wall breaking machine, and stirring to obtain paste;
mixing raw materials: adding 20g of peptone, 10g of activated carbon, 100g of white sugar and 10g of agar into the nutrient solution, adding mashed potato and banana, heating to 95 ℃, stirring, mixing, and filling into a culture bottle after stirring;
and (3) sterilization treatment: placing the culture bottle into a sterilizing pot, sterilizing at 130 deg.C for 50min, placing into a sterilizing cabinet or sterilizing cooling workshop, cooling, and getting the final product.
Example 3
This example provides an orchid culture medium prepared by the following steps:
preparing a nutrient solution: adding 900g of water into a nutrient mixture consisting of 1.5g of potassium nitrate, 1.2g of ammonium nitrate, 0.2g of monopotassium phosphate, 0.4g of magnesium sulfate and 0.5g of calcium chloride, and ultrasonically mixing for 25min at the frequency of 28KHZ to obtain a nutrient solution for later use;
material treatment: peeling 50g of potatoes, cleaning the potatoes under ultrasonic waves, cutting the potatoes into blocks, cooking the potatoes and 40g of bananas for 25min at 90 ℃, peeling the cooked potatoes and the bananas, putting the potatoes and the bananas into a wall breaking machine, crushing and stirring the potatoes into paste for later use;
mixing raw materials: adding 10g of peptone, 5g of activated carbon, 50g of white sugar and 5g of agar into the nutrient solution, adding mashed potato and banana, heating to 90 ℃, stirring and mixing, and filling into a culture bottle after stirring;
and (3) sterilization treatment: placing the culture bottle into a sterilizing pot, sterilizing at 125 deg.C for 30min, placing into a sterilizing cabinet or sterilizing cooling workshop, cooling, and getting the final product.
Example 4
This example provides an orchid culture medium prepared by the following steps:
preparing a nutrient solution: adding 1000g of water into a nutrient mixture consisting of 2g of potassium nitrate, 1.5g of ammonium nitrate, 0.25g of monopotassium phosphate, 0.35g of magnesium sulfate and 0.6g of calcium chloride, and then ultrasonically mixing for 20min at the frequency of 28KHZ to obtain a nutrient solution for later use;
material treatment: peeling 80g of potato, cleaning under ultrasonic, cutting into pieces, steaming at 90 deg.C for 25min, peeling the cooked potato and 50g of banana, pulverizing in a wall breaking machine, and stirring to obtain paste;
mixing raw materials: adding 12g of peptone, 7g of activated carbon, 30g of white sugar and 4g of agar into the nutrient solution, adding mashed potato and banana, heating to 92 ℃, stirring and mixing, and filling into a culture bottle after stirring;
and (3) sterilization treatment: placing the culture bottle into a sterilizing pot, sterilizing at 120 deg.C for 30min, placing into a sterilizing cabinet or sterilizing cooling workshop, cooling, and getting the final product.
Example 5
This example provides an orchid culture medium prepared by the following steps:
preparing a nutrient solution: adding 100g of water into a nutrient mixture consisting of 1.7g of potassium nitrate, 1.4g of ammonium nitrate, 0.23g of monopotassium phosphate, 0.45g of magnesium sulfate and 0.55g of calcium chloride, and ultrasonically mixing for 20min at the frequency of 28KHZ to obtain a nutrient solution for later use;
material treatment: peeling 80g of potato, cleaning under ultrasonic, cutting into pieces, steaming at 90 deg.C for 30min, peeling the cooked potato and 80g of banana, pulverizing in a wall breaking machine, and stirring to obtain paste;
mixing raw materials: adding 12g of peptone, 5g of activated carbon, 15g of white sugar and 8g of agar into the nutrient solution, adding mashed potato and banana, heating to 85 ℃, stirring, mixing, and filling into a culture bottle after stirring;
and (3) sterilization treatment: placing the culture bottle into a sterilizing pot, sterilizing at 125 deg.C for 45min, placing into a sterilizing cabinet or sterilizing cooling workshop, cooling, and getting the final product.
In summary, according to the culture medium for orchids and the preparation method thereof provided by the embodiments of the present invention, the potato is rich in nutrients such as carbohydrates, plant proteins, vitamin C, dietary fibers, etc., and the banana is rich in nutrients such as vitamin a, phosphorus, thiamine, riboflavin, potassium, magnesium, etc., which can provide nutrients required by orchids for tissue culture, and are beneficial for growth and propagation of orchids; the peptone is rich in organic nitrogen compounds, vitamins and saccharides, contains rich amino acids, particularly more sulfur-containing amino acids, can provide a nitrogen source for tissue culture of orchidaceae plants, and is beneficial to growth and reproduction of plant tissues; the activated carbon has adsorption effect and can adsorb some harmful impurities, thereby reducing the influence of the harmful impurities on the growth of the tissue of the orchid and being beneficial to the growth and the propagation of the tissue of the orchid; the white sugar is used as a carbon source, can provide energy for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The agar can be used as a coagulator, which is beneficial to the use of a culture medium; the nutrient mixture contains various nutrient elements, can provide sufficient nutrition for the growth of the tissue of the orchidaceae plant, and is beneficial to the growth and the propagation of the orchidaceae plant. The orchid culture medium composed of the components is adjusted on the formula of the orchid plant, and other common raw materials are used for providing nutrition for tissues of the orchid plant to enable the orchid plant to grow and reproduce, so that the orchid culture medium is good in effect and convenient to use. The preparation method comprises the steps of firstly preparing the nutrient solution through the nutrient solution preparation step, adding water into the nutrient mixture, and mixing the nutrient mixture and the water through ultrasound, so that the nutrient mixture and the water can be more fully mixed, and the obtained nutrient solution is favorable for improving the effect of nutrients on tissue culture of orchids; then, the potatoes and the bananas are processed through the material processing steps, the potatoes are peeled and cut into blocks for cooking, the cutting into blocks can reduce the time required for cooking the potatoes, and some toxic substances in the potatoes can be removed through cooking, so that the growth and the propagation of orchid tissues are facilitated, meanwhile, the texture of the potatoes can be softened, the potatoes and the peeled bananas are conveniently put into a wall breaking machine together for crushing and stirring into mud in the subsequent steps, and the use is more convenient; through the raw material mixing step, peptone, activated carbon, white sugar and agar are added into the nutrient solution, and mashed potatoes and bananas are added, and the nutrient solution, the peptone, the activated carbon, the white sugar, the agar, the mashed potatoes and the bananas can be mixed more fully through heating and stirring, so that the mutual combination and the synergistic effect of the components are facilitated, the culture effect of the orchid plant tissue is improved, and the orchid plant tissue is more convenient to use; finally, through the sterilization treatment steps, the culture bottle is placed into a sterilization pot for sterilization, and is placed into a disinfection cabinet or a disinfection cooling workshop for cooling after the sterilization is finished, so that harmful strains and impurities in the culture bottle are removed, the toxicity of the harmful strains and the impurities is reduced, the culture effect on the orchid tissue is improved, the growth and the propagation of the orchid tissue are facilitated, and the use is more convenient. The whole preparation method has simple flow, the prepared culture medium has good culture effect, is beneficial to the growth and the propagation of the tissue of the orchids, and is convenient to use.
The embodiments described above are some, but not all embodiments of the invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (10)
1. An orchid culture medium is characterized by comprising the following components: banana, potato, peptone, activated carbon, white sugar, agar and nutrient mixture and water.
2. The orchid culture medium according to claim 1, wherein the orchid culture medium comprises the following components in parts by weight: 2-80 parts of banana, 2-100 parts of potato, 1-20 parts of peptone, 0.5-10 parts of activated carbon, 1-100 parts of white sugar, 1-10 parts of agar, 1.6-6.2 parts of nutrient and 800 parts of water-activated sugar.
3. The orchid culture medium according to any one of claims 1-2, wherein the nutrient mixture comprises the following components: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride.
4. The orchid culture medium according to claim 3, wherein the nutrient mixture comprises the following components in parts by weight: 0.5-2.5 parts of potassium nitrate, 0.5-2 parts of ammonium nitrate, 0.1-0.3 part of monopotassium phosphate, 0.2-0.6 part of magnesium sulfate and 0.3-0.8 part of calcium chloride.
5. The orchid culture medium as claimed in claim 1, wherein the water is deionized water.
6. A method for preparing the orchid culture medium according to any one of claims 1 to 5, comprising the following steps:
preparing a nutrient solution: adding water into the nutrient mixture, and then mixing the nutrient mixture by ultrasonic to obtain a nutrient solution for later use;
material treatment: peeling potatoes, cleaning the potatoes under ultrasonic waves, cutting the potatoes into blocks, cooking the blocks, putting the cooked potatoes and peeled bananas into a wall breaking machine, crushing and stirring the potatoes and the peeled bananas into mud for later use;
mixing raw materials: adding peptone, activated carbon, white sugar and agar into the nutrient solution, adding mashed potato and banana, heating while stirring for mixing, and putting into a culture bottle after stirring;
and (3) sterilization treatment: and (4) placing the culture bottle into a sterilization pot for sterilization, placing the culture bottle into a sterilization cabinet or a sterilization cooling workshop for cooling after the sterilization is finished, and obtaining a finished product after the cooling is finished.
7. The method for preparing the orchid culture medium according to claim 6, wherein the nutrient solution is ultrasonically mixed at a frequency of 28KHZ for 15-30min during the preparation process.
8. The method for preparing the orchid culture medium according to claim 6, wherein the potatoes are cut into pieces and cooked at 80-95 ℃ for 15-30min during the material treatment.
9. The method for producing an orchid culture medium according to claim 6, wherein the raw materials are mixed while being heated to 80-95 ℃ under stirring.
10. The method for preparing the culture medium of Orchidaceae plant as claimed in claim 6, wherein the sterilization treatment is carried out by placing the culture flask in a sterilization pot and sterilizing at 120-130 deg.C for 15-50 min.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115211372A (en) * | 2022-08-23 | 2022-10-21 | 湖南省园艺研究所 | Culture medium applied to tissue culture of cymbidium and processing method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
| CN103718948A (en) * | 2013-12-06 | 2014-04-16 | 广西壮族自治区林业科学研究院 | Cultivation method of new germplasm of ornamental Dendrobium spp. |
| CN104145815A (en) * | 2014-07-25 | 2014-11-19 | 庄国钟 | Dendrobium candidum culture medium and preparation method thereof |
| CN104823856A (en) * | 2015-05-18 | 2015-08-12 | 江苏丰收大地种业发展有限公司 | Rapid proliferation culture medium of dendrobium candidum tissue culture seedlings |
| US20160143236A1 (en) * | 2013-07-08 | 2016-05-26 | South China Botanical Garden, Chinese Academy Of Sciences | Dendrobium in vitro crossbreeding method |
-
2020
- 2020-11-30 CN CN202011379771.8A patent/CN112470924A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20160143236A1 (en) * | 2013-07-08 | 2016-05-26 | South China Botanical Garden, Chinese Academy Of Sciences | Dendrobium in vitro crossbreeding method |
| CN103416305A (en) * | 2013-07-19 | 2013-12-04 | 福建省农业科学院农业生物资源研究所 | Hormone-free tissue culture and rapid propagation method of anoectochilus formosanus seedlings |
| CN103718948A (en) * | 2013-12-06 | 2014-04-16 | 广西壮族自治区林业科学研究院 | Cultivation method of new germplasm of ornamental Dendrobium spp. |
| CN104145815A (en) * | 2014-07-25 | 2014-11-19 | 庄国钟 | Dendrobium candidum culture medium and preparation method thereof |
| CN104823856A (en) * | 2015-05-18 | 2015-08-12 | 江苏丰收大地种业发展有限公司 | Rapid proliferation culture medium of dendrobium candidum tissue culture seedlings |
Non-Patent Citations (2)
| Title |
|---|
| 冉懋雄: "《中药组织培养实用技术》", 31 August 2004, 北京科学技术文献出版社 * |
| 陈宇勒: "《兰花繁育技术图解》", 30 November 2009, 广东科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115211372A (en) * | 2022-08-23 | 2022-10-21 | 湖南省园艺研究所 | Culture medium applied to tissue culture of cymbidium and processing method thereof |
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