CN112462081A - Full-automatic biological sample preservation analysis control system - Google Patents
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- 239000012472 biological sample Substances 0.000 title claims abstract description 33
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G05B19/41865—Total factory control, i.e. centrally controlling a plurality of machines, e.g. direct or distributed numerical control [DNC], flexible manufacturing systems [FMS], integrated manufacturing systems [IMS] or computer integrated manufacturing [CIM] characterised by job scheduling, process planning, material flow
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Abstract
A full-automatic biological sample preservation analysis control system comprises a main control unit, a motor driving module, a stepping motor, a sensor, a lithium battery, a power management module, an ultrasonic generation module and a semiconductor temperature control module; the ultrasonic generation module and the semiconductor temperature control module are in signal connection with the main control unit, and the main control unit is in control connection with the sensor and the motor driving module; the ultrasonic generation module is in control connection with the motor driving module, and the motor driving module is in control connection with the stepping motor; the power management module is connected with the lithium battery in a control mode, and the lithium battery is respectively and electrically connected with the main control unit, the motor driving module and the semiconductor temperature control module. The invention can realize the long-time preservation of the biological sample, and the inspection and detection, and the automatic control of the whole flow of preservation, transportation, uniform mixing, reaction and detection of the biological sample; the control system is used for realizing the control of the miniaturized automatic equipment of the biological samples, and meanwhile, cross contamination can be avoided by using different motors in different areas, and the cost is low.
Description
Technical Field
The invention relates to the technical field of biological detection, in particular to a full-automatic biological sample storage analysis control system.
Background
With the development of biotechnology, biological samples are more and more concerned about preservation, treatment and detection, the biological samples are usually preserved at-4 ℃, the treatment of the biological samples includes temperature increase and decrease, sample mixing, sample transfer, obtaining related products through various reactions after the treated samples, and detecting the products, thereby realizing the analysis and detection of the biological samples, and the miniaturization and automatic detection of the analysis of the biological samples are the development direction of the biological analysis and detection technology. Most of the bioanalytical systems currently used in test assays are large devices that, despite high test throughput, are expensive and have limited flexibility of application. In contrast, the miniaturized preservation automation biological analysis system has the advantages of small sample usage amount, full flow automation, rapid analysis and simple and convenient operation, and is a great revolution to the existing operation mode and equipment. Compared with a large biological analysis instrument, the miniaturized preservation automatic biological analysis system has the advantages of convenient use, modular combination, low price and more flexible application, thereby being very suitable for on-site, emergency treatment, small and medium-sized inspection and detection mechanisms, medium and small-sized medical mechanisms and the like. The development of the system is an urgent need of biological sample inspection work, can effectively reduce the detection cost, is beneficial to improving the popularization of biotechnology in the whole society, improves the service quality and better meets the requirements of the masses on biological inspection and detection; in order to solve the above problems, the present application provides a fully automatic biological sample preservation analysis control system.
Disclosure of Invention
Objects of the invention
In order to solve the technical problems in the background technology, the invention provides a full-automatic biological sample storage analysis control system which can realize the long-time storage of biological samples, carry out inspection and detection at selected time and realize the automatic control of the whole flow of storage, transportation, uniform mixing, reaction and detection of the biological samples. The scheme is used for realizing the control of the miniaturized automatic equipment of the biological samples, and meanwhile, different motors are used in different areas to avoid cross contamination. The scheme has low cost and is easy to popularize.
(II) technical scheme
The invention provides a full-automatic biological sample storage analysis control system, which comprises a main control unit, a motor driving module, a stepping motor, a sensor, a lithium battery, a power management module, an ultrasonic generation module and a semiconductor temperature control module, wherein the main control unit is connected with the motor driving module;
the ultrasonic generation module and the semiconductor temperature control module are in signal connection with the main control unit, and the main control unit is in control connection with the sensor and the motor driving module; the ultrasonic generation module is in control connection with the motor driving module, and the motor driving module is in control connection with the stepping motor; the power management module is in control connection with the lithium battery, and the lithium battery is respectively and electrically connected with the main control unit, the motor driving module and the semiconductor temperature control module;
the main control unit circuit adopts STM32F767IGT6 to output 4 paths of stepping motor pulse signals and motor running direction signals, control 4 stepping motors to run to accurate positions, detect temperature values of 2 temperature zones and output 2 paths of temperature control PWM signals to control the temperature of 2 temperature zones of the reaction chip;
the power management module comprises a chip TPS54331, a chip LDO and a chip REF 3030;
the chip TPS54331 is used for outputting DC5v from DC15v to supply power for the driving of the stepping motor;
the chip LDO is used for outputting 3.3V from DC5V to the main control unit;
the chip REF3030 is used for providing a stable 3.0V reference power supply for the sensor;
the motor driving module is used for controlling the stepping motor and is provided with a photoelectric switch signal;
the stepping motor is LV8728MR to drive a two-phase stepping motor, and the moving distance is controlled by isolating a stepping motor control signal through an optical coupler and an IO output port of the main control unit; the high and low levels of the running direction signals of the stepping motor are output by an io port of the main control unit to control the forward rotation and the reverse rotation of the motor;
the photoelectric switch signal is used for setting the operation zero position of the motor, and the transportation and the uniform mixing of the biological sample and the detection of the optical signal are realized through the control of the main control unit on the motor;
the ultrasonic generation module is used for receiving the control signal of the main control unit stepping motor and outputting PWM pulse;
the semiconductor temperature control module comprises a mos tube fds 8638;
the semiconductor temperature control module controls the on-off of the mos tube fds8638 through the PWM signal output by the main control unit to control the on-off of the heating film power supply, so that the reaction chip is heated through the heating film, the main control unit calculates a temperature value according to the characteristic that the PT100 resistance value changes along with the temperature, and the main control unit controls the pulse width of PWM through a PID algorithm, so that the temperature required by biological preservation and reaction is controlled;
the sensor is a PT100 sensor and is used for detecting the resistance value of the temperature sensor PT100 in a bridge mode, amplifying the signal through an AD623 and sending the signal to an AD input port of the main control unit.
Preferably, the master control unit employs a processor of stm 32.
Preferably, the kit further comprises a fluorescence generation and detection module; the fluorescence generation and detection module is connected with the main control unit through a serial port.
Preferably, the system also comprises a human-computer interface; the human-computer interface is connected with the main control unit through a serial port.
Preferably, the human-computer interface adopts a 7-inch LCD touch screen.
Preferably, the operating speed of the stepper motor is controlled by varying the frequency of the PWM pulses.
The technical scheme of the invention has the following beneficial technical effects: DC15V outputs DC5V to supply power for a stepping motor through a switch power chip TPS54331, DC5V outputs 3.3V to supply power for a main control unit through an LDO chip, the main control unit adopts an STM32 processor to accurately acquire parameters such as sample displacement, sample environment temperature and the like in real time, LV8728MR is adopted to drive a 2-phase stepping motor, a control signal of the stepping motor is isolated from an IO output port of a microprocessor through an optical coupler, a timer of the microprocessor outputs PWM (pulse-width modulation) pulses to control the moving distance of the stepping signal (such as STEP4), the frequency of the PWM pulses is changed to change the operating speed of the motor, the transmission of the sample is realized, an STM32F767IGT6 is adopted as a main control circuit, 4 paths of stepping motor pulse signals and motor operating direction signals are output, 4 stepping motors are controlled to operate to accurate positions, temperature values of 2 temperature zones are detected, 2 temperature control PWM signals are output to control the temperature of 2 temperature zones of a reaction chip, and the uniform mixing of biological samples, refrigeration and heating are realized by controlling the on-off of the mos tube fds8638 through the PWM signal output by the microprocessor to control the on-off of the heating film power supply, so that the reaction chip is heated through the heating film, and the temperature of the reaction chip is detected through the PT100 sensor. REF3030 provides a stable 3.0V reference power supply, the resistance value of a temperature sensor PT100 is detected in a bridge mode, signals are amplified through an AD623 and then sent to an AD input port of a microprocessor, the microprocessor calculates a temperature value according to the characteristic that the resistance value of the PT100 changes along with the temperature, the microprocessor controls the pulse width of PWM through a PID algorithm, so that the temperature required by biological preservation and reaction is controlled, an environment suitable for sample preservation and reaction is provided, an optical excitation and optical acquisition system is controlled according to the quantity of biological samples, biological signal collection is realized, the collected signals are processed through the processor, and a 7-inch LCD touch screen is adopted through a human-computer interface to display a data curve of a detection result; and alarming and prompting the emergency or abnormal condition.
Drawings
Fig. 1 is a block diagram of a control system of a fully automatic biological sample preservation and analysis control system according to the present invention.
Fig. 2 is a block diagram of a main control system in a full-automatic biological sample preservation and analysis control system according to the present invention.
Fig. 3 is a block diagram of a transmission and mixing control system in a full-automatic biological sample storage and analysis control system provided by the invention.
Fig. 4 is a block diagram of a power control system in a full-automatic biological sample preservation analysis control system according to the present invention.
Fig. 5 is a block diagram of a temperature rise and decrease and detection control system in a full-automatic biological sample storage analysis control system according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings in conjunction with the following detailed description. It should be understood that the description is intended to be exemplary only, and is not intended to limit the scope of the present invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
As shown in fig. 1-5, the full-automatic biological sample storage, analysis and control system provided by the present invention comprises a main control unit, a motor driving module, a stepping motor, a sensor, a lithium battery, a power management module, an ultrasonic generation module and a semiconductor temperature control module;
the ultrasonic generation module and the semiconductor temperature control module are in signal connection with the main control unit, and the main control unit is in control connection with the sensor and the motor driving module; the ultrasonic generation module is in control connection with the motor driving module, and the motor driving module is in control connection with the stepping motor; the power management module is in control connection with the lithium battery, and the lithium battery is respectively and electrically connected with the main control unit, the motor driving module and the semiconductor temperature control module;
the main control unit circuit adopts STM32F767IGT6 to output 4 paths of stepping motor pulse signals and motor running direction signals, control 4 stepping motors to run to accurate positions, detect temperature values of 2 temperature zones and output 2 paths of temperature control PWM signals to control the temperature of 2 temperature zones of the reaction chip;
the power management module comprises a chip TPS54331, a chip LDO and a chip REF 3030;
the chip TPS54331 is used for outputting DC5v from DC15v to supply power for the driving of the stepping motor;
the chip LDO is used for outputting 3.3V from DC5V to the main control unit;
the chip REF3030 is used for providing a stable 3.0V reference power supply for the sensor;
the motor driving module is used for controlling the stepping motor and is provided with a photoelectric switch signal;
the stepping motor is LV8728MR to drive a two-phase stepping motor, and the moving distance is controlled by isolating a stepping motor control signal through an optical coupler and an IO output port of the main control unit; the high and low levels of the running direction signals of the stepping motor are output by an io port of the main control unit to control the forward rotation and the reverse rotation of the motor;
the photoelectric switch signal is used for setting the operation zero position of the motor, and the transportation and the uniform mixing of the biological sample and the detection of the optical signal are realized through the control of the main control unit on the motor;
the ultrasonic generation module is used for receiving the control signal of the main control unit stepping motor and outputting PWM pulse;
the semiconductor temperature control module comprises a mos tube fds 8638;
the semiconductor temperature control module controls the on-off of the mos tube fds8638 through the PWM signal output by the main control unit to control the on-off of the heating film power supply, so that the reaction chip is heated through the heating film, the main control unit calculates a temperature value according to the characteristic that the PT100 resistance value changes along with the temperature, and the main control unit controls the pulse width of PWM through a PID algorithm, so that the temperature required by biological preservation and reaction is controlled;
the sensor is a PT100 sensor and is used for detecting the resistance value of the temperature sensor PT100 in a bridge mode, amplifying the signal through an AD623 and sending the signal to an AD input port of the main control unit.
In an alternative embodiment, the master control unit employs a processor of stm 32.
In an optional embodiment, the kit further comprises a fluorescence generation and detection module; the fluorescence generation and detection module is connected with the main control unit through a serial port.
In an optional embodiment, the system further comprises a human-computer interface; the human-computer interface is connected with the main control unit through a serial port.
In an alternative embodiment, the human-machine interface is a 7-inch LCD touch screen.
In an alternative embodiment, the operating speed of the stepper motor is controlled by varying the frequency of the PWM pulses.
In the invention, DC15V outputs DC5V to supply power to a stepping motor through a switch power supply chip TPS54331, DC5V outputs 3.3V to supply power to a main control unit through an LDO chip, the main control unit adopts a STM32 processor to accurately acquire parameters such as sample displacement, sample environment temperature and the like in real time, LV8728MR is adopted to drive a 2-phase stepping motor, a control signal of the stepping motor is isolated from an IO output port of a microprocessor through an optical coupler, a timer of the microprocessor outputs PWM pulse to control the moving distance of a stepping signal (such as STEP4), the frequency of the PWM pulse is changed to change the operating speed of the motor, the transmission of the sample is realized, an STM32F767IGT6 is adopted as a main control circuit, 4 paths of stepping motor pulse signals and motor operating direction signals are output, 4 stepping motors are controlled to operate to accurate positions, temperature values of 2 temperature zones are detected, and 2 paths of temperature control PWM signals are output to control the temperature of 2, the mixing of biological samples is realized through the control to the motor to the supersound module, thereby refrigeration and heating are through microprocessor output PWM signal control mos pipe fds 8638's break-make to control the switch of heating the membrane power and heat the reaction chip through heating the membrane, and the temperature detects the temperature that detects the reaction chip through the PT100 sensor. REF3030 provides a stable 3.0V reference power supply, the resistance value of a temperature sensor PT100 is detected in a bridge mode, signals are amplified through an AD623 and then sent to an AD input port of a microprocessor, the microprocessor calculates a temperature value according to the characteristic that the resistance value of the PT100 changes along with the temperature, the microprocessor controls the pulse width of PWM through a PID algorithm, so that the temperature required by biological preservation and reaction is controlled, an environment suitable for sample preservation and reaction is provided, an optical excitation and optical acquisition system is controlled according to the quantity of biological samples, biological signal collection is realized, the collected signals are processed through the processor, and a 7-inch LCD touch screen is adopted through a human-computer interface to display a data curve of a detection result; and alarming and prompting the emergency or abnormal condition.
It is to be understood that the above-described embodiments of the present invention are merely illustrative of or explaining the principles of the invention and are not to be construed as limiting the invention. Therefore, any modification, equivalent replacement, improvement and the like made without departing from the spirit and scope of the present invention should be included in the protection scope of the present invention. Further, it is intended that the appended claims cover all such variations and modifications as fall within the scope and boundaries of the appended claims or the equivalents of such scope and boundaries.
Claims (6)
1. A full-automatic biological sample preservation, analysis and control system is characterized by comprising a main control unit, a motor driving module, a stepping motor, a sensor, a lithium battery, a power management module, an ultrasonic generation module and a semiconductor temperature control module;
the ultrasonic generation module and the semiconductor temperature control module are in signal connection with the main control unit, and the main control unit is in control connection with the sensor and the motor driving module; the ultrasonic generation module is in control connection with the motor driving module, and the motor driving module is in control connection with the stepping motor; the power management module is in control connection with the lithium battery, and the lithium battery is respectively and electrically connected with the main control unit, the motor driving module and the semiconductor temperature control module;
the main control unit circuit adopts STM32F767IGT6 to output 4 paths of stepping motor pulse signals and motor running direction signals, control 4 stepping motors to run to accurate positions, detect temperature values of 2 temperature zones and output 2 paths of temperature control PWM signals to control the temperature of 2 temperature zones of the reaction chip;
the power management module comprises a chip TPS54331, a chip LDO and a chip REF 3030;
the chip TPS54331 is used for outputting DC5v from DC15v to supply power for the driving of the stepping motor;
the chip LDO is used for outputting 3.3V from DC5V to the main control unit;
the chip REF3030 is used for providing a stable 3.0V reference power supply for the sensor;
the motor driving module is used for controlling the stepping motor and is provided with a photoelectric switch signal;
the stepping motor is LV8728MR to drive a two-phase stepping motor, and the moving distance is controlled by isolating a stepping motor control signal through an optical coupler and an IO output port of the main control unit; the high and low levels of the running direction signals of the stepping motor are output by an io port of the main control unit to control the forward rotation and the reverse rotation of the motor;
the photoelectric switch signal is used for setting the operation zero position of the motor, and the transportation and the uniform mixing of the biological sample and the detection of the optical signal are realized through the control of the main control unit on the motor;
the ultrasonic generation module is used for receiving the control signal of the main control unit stepping motor and outputting PWM pulse;
the semiconductor temperature control module comprises a mos tube fds 8638;
the semiconductor temperature control module controls the on-off of the mos tube fds8638 through the PWM signal output by the main control unit to control the on-off of the heating film power supply, so that the reaction chip is heated through the heating film, the main control unit calculates a temperature value according to the characteristic that the PT100 resistance value changes along with the temperature, and the main control unit controls the pulse width of PWM through a PID algorithm, so that the temperature required by biological preservation and reaction is controlled;
the sensor is a PT100 sensor and is used for detecting the resistance value of the temperature sensor PT100 in a bridge mode, amplifying the signal through an AD623 and sending the signal to an AD input port of the main control unit.
2. The system of claim 1, wherein the main control unit employs a processor of stm 32.
3. The system of claim 1, further comprising a fluorescence generation and detection module; the fluorescence generation and detection module is connected with the main control unit through a serial port.
4. The system of claim 1, further comprising a human-machine interface; the human-computer interface is connected with the main control unit through a serial port.
5. The system of claim 4, wherein the human-computer interface is a 7-inch LCD touch screen.
6. The system of claim 1, wherein the operation speed of the stepping motor is controlled by varying the frequency of the PWM pulses.
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