CN112457070A - Environment-friendly biological agent for treating straws - Google Patents
Environment-friendly biological agent for treating straws Download PDFInfo
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- CN112457070A CN112457070A CN202011291659.9A CN202011291659A CN112457070A CN 112457070 A CN112457070 A CN 112457070A CN 202011291659 A CN202011291659 A CN 202011291659A CN 112457070 A CN112457070 A CN 112457070A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Soil Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to an environment-friendly biological agent for treating straws, which comprises 8-13 parts of alternaria powder, 10-15 parts of trichoderma viride powder, 13-16 parts of galacturonase, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of soybean rhizobium powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of high-temperature pseudomonas fuscogens powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino-oligosaccharin and 0.05-1 part of brassinolide; the method can accelerate the decomposition of cellulose and hemicellulose in the straws, has low requirement on working conditions, can start self-fermentation at normal temperature, does not need high-temperature high-pressure catalysis, greatly shortens the treatment period to 24 hours to a week, is environment-friendly and harmless, and finally treats the product as biological bacterial manure, thereby changing waste into valuable.
Description
Technical Field
The invention relates to the field of microbial agents, in particular to an environment-friendly biological agent for treating straws.
Background
In recent years, with the popularization and application of various new cultivation technologies, the crop yield is greatly improved, and the number of straws is increased. Except for partially using the straws for feed, retting and deep processing, most of the straws are abandoned in the field or directly burned, and the waste of straw resources is very serious. The method for decomposing straws and returning the straws to the field by applying the microbial technology is a novel efficient and environment-friendly crop waste resource recycling mode, and is widely popularized and applied nationwide at present. After the microbial inoculum is used for returning straws to the field, the microbial inoculum plays an important role in the utilization of straw resources, corrects the waste phenomenon of burning straws to a great extent, reduces atmospheric pollution and ecological damage, simultaneously fertilizes soil fertility, and effectively improves the organic matter content of soil.
The straw mainly comprises cellulose, hemicellulose, lignin and the like, and is difficult to decompose by microorganisms under natural conditions; the microbial agent product for straw degradation in the current market has the defects of single fungus, low degradation efficiency and weak decomposition capability on a cellulose tissue structure to a great extent.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide an environment-friendly biological agent for treating straws.
In order to achieve the purpose, the invention adopts the technical scheme that: an environment-friendly biological agent for treating straws comprises, by weight, 8-13 parts of alternaria powder, 10-15 parts of trichoderma viride powder, 13-16 parts of galacturonase, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of soybean rhizobium powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of pseudomonas fuscogramma powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino-oligosaccharide and 0.05-1 part of brassinolide.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
1. the invention can accelerate the decomposition of cellulose and hemicellulose in the straws, thereby achieving the purpose of quickly decomposing the straws, greatly shortening the treatment period to 24 hours to one week, having mild reaction conditions, no need of a high-temperature high-pressure container, starting fermentation and degradation at normal temperature and normal pressure, having simple reaction site, being applicable to rural cities, and only needing a basic stirring and heat-preserving device;
2. all the strains adopted by the invention do not contain pathogenic bacteria, have the function of regulating and repairing soil, are fermented into nutrient soil after the degradation of pollutants is finished, and can be returned to the field, thereby really realizing the resource treatment of garbage.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
The first embodiment is as follows:
the environment-friendly biological agent for treating straws comprises, by weight, 13 parts of alternaria powder, 15 parts of trichoderma viride powder, 16 parts of galacturonase, 10 parts of lactobacillus powder, 10 parts of bacillus megaterium powder, 12 parts of bacillus subtilis powder, 9 parts of bacillus amyloliquefaciens powder, 5 parts of saccharomyces cerevisiae powder, 1 part of bacillus mucilaginosus powder, 1 part of soybean rhizobia powder, 2 parts of azotobacter chroococcum powder, 3 parts of pseudomonas fusca powder, 1 part of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.01 part of 90% amino-oligosaccharide and 0.09 part of brassinolide.
A preparation method of an environment-friendly biological agent for treating straws comprises the following steps:
s1: generating flora: adding alternaria alternate powder, trichoderma viride powder, galacturonase, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, taking 100kg of fermentation as an example, additionally adding 10kg of peptone, 10kg of lactobacillus fermentation mother liquor, 1kg of brown sugar and 1kg of urea, and performing constant-temperature fermentation at 37 ℃ for 10 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 10kg of wort, 1kg of monohydrogenphosphate and 1kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 0.3kg of calcium carbonate and 0.5kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 24h on a shaking table at the speed of 100 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 5kg of corn germ meal, 10kg of tenebrio molitor sand and 5kg of corn bran into the microbial inoculum, carrying out 1-day industrial fermentation propagation at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
Example two:
the environment-friendly biological agent for treating straws comprises, by weight, 10 parts of alternaria powder, 13 parts of trichoderma viride powder, 13 parts of galacturonase, 9 parts of lactobacillus powder, 9 parts of bacillus megaterium powder, 10 parts of bacillus subtilis powder, 12 parts of bacillus amyloliquefaciens powder, 6 parts of saccharomyces cerevisiae powder, 3 parts of bacillus mucilaginosus powder, 3 parts of soybean rhizobia powder, 3 parts of azotobacter chroococcum powder, 5 parts of pseudomonas fusca powder, 2 parts of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.03 part of 90% amino-oligosaccharide and 0.07 part of brassinolide.
A preparation method of an environment-friendly biological agent for treating straws comprises the following steps:
s1: generating flora: adding alternaria alternate powder, trichoderma viride powder, galacturonase, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, taking 100kg of fermentation as an example, adding 12kg of peptone, 15kg of lactobacillus fermentation mother liquor, 1.5kg of brown sugar and 1.5kg of urea, and performing constant-temperature fermentation at 37 ℃ for 12 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 15kg of wort, 2kg of monohydrogenphosphate and 1.5kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 0.5kg of calcium carbonate and 0.8kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 18h on a shaker at the speed of 120 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 8kg of corn germ meal, 11kg of tenebrio molitor sand and 6kg of corn bran into the microbial inoculum, carrying out industrial fermentation propagation for 2 days at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
Example three:
the environment-friendly biological agent for treating straws comprises, by weight, 8 parts of alternaria powder, 10 parts of trichoderma viride powder, 13 parts of galacturonase, 8 parts of lactobacillus powder, 7 parts of bacillus megaterium powder, 10 parts of bacillus subtilis powder, 12 parts of bacillus amyloliquefaciens powder, 8 parts of saccharomyces cerevisiae powder, 5 parts of bacillus mucilaginosus powder, 5 parts of soybean rhizobia powder, 4 parts of azotobacter chroococcum powder, 5 parts of pseudomonas fusca powder, 3 parts of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.01 part of 90% amino-oligosaccharide and 0.09 part of brassinolide.
A preparation method of an environment-friendly biological agent for treating straws comprises the following steps:
s1: generating flora: adding alternaria alternate powder, trichoderma viride powder, galacturonase, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, taking 100kg of fermentation as an example, additionally adding 15kg of peptone, 20kg of lactobacillus fermentation mother liquor, 2kg of brown sugar and 2kg of urea, and carrying out constant-temperature fermentation at 37 ℃ for 14 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 20kg of wort, 3kg of monohydrogenphosphate and 2kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 1kg of calcium carbonate and 1kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 10 hours on a shaking table at the speed of 150 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 10kg of corn germ meal, 12kg of tenebrio molitor sand and 8kg of corn bran into the microbial inoculum, carrying out industrial fermentation propagation for 3 days at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
The environment-friendly biological agent obtained by the three embodiments can accelerate the decomposition of cellulose and hemicellulose in the straws, thereby achieving the purpose of quickly decomposing the straws, the treatment period is greatly shortened to 24 hours to one week, the reaction condition is mild, a high-temperature high-pressure container is not needed, the fermentation and degradation can be started at normal temperature and normal pressure, the reaction field is simple, the environment can be applied to rural cities, only a basic stirring and heat preservation device is needed, all strains do not contain pathogenic bacteria, the environment-friendly biological agent has the effect of adjusting and repairing soil, pollutants are degraded and then fermented into nutrient soil, the nutrient soil can be returned to the field, and the resource treatment of garbage is really realized.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (1)
1. An environment-friendly biological agent for treating straws is characterized in that: the composition comprises, by weight, 8-13 parts of alternaria powder, 10-15 parts of trichoderma viride powder, 13-16 parts of galacturonase, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of soybean rhizobium powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of pseudomonas fuscogramma powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino oligosaccharin and 0.05-1 part of brassinolide.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011291659.9A CN112457070A (en) | 2020-11-18 | 2020-11-18 | Environment-friendly biological agent for treating straws |
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| CN202011291659.9A CN112457070A (en) | 2020-11-18 | 2020-11-18 | Environment-friendly biological agent for treating straws |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113896581A (en) * | 2021-10-19 | 2022-01-07 | 上海玖沁环保科技有限公司 | Agricultural straw degradation treatment equipment |
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| CN104446709A (en) * | 2014-12-13 | 2015-03-25 | 张秀丽 | Biological fermentation organic fertilizer and preparation method thereof |
| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
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| CN108531416A (en) * | 2018-02-26 | 2018-09-14 | 佛山杰致信息科技有限公司 | A kind of environment-friendly stalk leavening and preparation method thereof |
-
2020
- 2020-11-18 CN CN202011291659.9A patent/CN112457070A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694410A (en) * | 2013-12-06 | 2015-06-10 | 大连三科生物工程有限公司 | Preparation method of straw decomposition agent |
| CN104446709A (en) * | 2014-12-13 | 2015-03-25 | 张秀丽 | Biological fermentation organic fertilizer and preparation method thereof |
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| CN108531416A (en) * | 2018-02-26 | 2018-09-14 | 佛山杰致信息科技有限公司 | A kind of environment-friendly stalk leavening and preparation method thereof |
Non-Patent Citations (1)
| Title |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113896581A (en) * | 2021-10-19 | 2022-01-07 | 上海玖沁环保科技有限公司 | Agricultural straw degradation treatment equipment |
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Application publication date: 20210309 |