CN112442444A - Novel nucleic acid amplification optical detection system - Google Patents
Novel nucleic acid amplification optical detection system Download PDFInfo
- Publication number
- CN112442444A CN112442444A CN202010480238.4A CN202010480238A CN112442444A CN 112442444 A CN112442444 A CN 112442444A CN 202010480238 A CN202010480238 A CN 202010480238A CN 112442444 A CN112442444 A CN 112442444A
- Authority
- CN
- China
- Prior art keywords
- optical
- module
- optical detection
- nucleic acid
- acid amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/13—Moving of cuvettes or solid samples to or from the investigating station
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a novel optical detection system for nucleic acid amplification, which comprises: amplification module, optical detection head, motion, right side opto-coupler, optical filtering module, signal processing analysis module, control module, left side opto-coupler, right side anti-dazzling screen, left side anti-dazzling screen wherein: the amplification module is provided with test tube hole sites which are arranged in a staggered manner; the optical detection head is a Y-shaped optical fiber; the motion mechanism comprises: the device comprises a stepping motor, a screw rod, a linear guide rail and a sliding block; the optical filtering module includes: the device comprises an optical fixing seat, an exciting light filter, an exciting light lens, a detection filter and a detection lens; the signal processing and analyzing module comprises: the device comprises a light source, a photoelectric detection element, a signal processing and analyzing circuit board and a shielding case; the control module is used for controlling the motion mechanism to perform back-and-forth scanning motion; the invention can greatly shorten the detection time for acquiring fluorescence data by adopting a hole site-by-hole site scanning mode, reduce the system cost and improve the system stability.
Description
Technical Field
The invention belongs to the field of molecular diagnosis, and relates to a novel optical detection system for nucleic acid amplification.
Background
The nucleic acid amplification detection technology is a process of providing in vitro amplification conditions for nucleic acid fragments, enabling the nucleic acid fragments to be amplified in an exponential manner in a large quantity, adding a fluorescent dye or a fluorescent marker in the nucleic acid amplification process, detecting the intensity of a fluorescent signal by adopting an optical device, and obtaining a nucleic acid amplification result by analyzing the fluorescent signal. Mainly comprises real-time fluorescence quantitative PCR (polymerase chain reaction) technology, constant temperature nucleic acid amplification detection technology and the like.
At present, the domestic fluorescent nucleic acid amplification detection technology mainly comprises the following steps:
shooting the amplification module for multiple times by adopting a CCD shooting technology to detect the fluorescence signal of the amplification module;
the bottom of the amplification module is scanned one by adopting an optical detection element to obtain fluorescence data.
The method of scanning the hole sites one by adopting the optical detection element has the advantages that each hole site of the amplification module needs to be scanned, the required fluorescence data acquisition time is long, the detection consistency is poor, and the technical requirements of specific fields such as high-resolution dissolution curve experiments and the like cannot be met. Meanwhile, the scanning detection mode in the X-axis direction and the Y-axis direction has high requirement on the positioning precision of the movement mechanism, and the precision error of the positioning mechanism can reduce the stability and the accuracy of the nucleic acid amplification analysis detection system.
Disclosure of Invention
In order to solve the defects of the prior art, the invention provides a novel nucleic acid amplification optical detection system, which can greatly shorten the detection time for acquiring fluorescence data by adopting a hole site-by-hole site scanning mode, reduce the system cost and improve the system stability.
A novel optical detection system for nucleic acid amplification, comprising:
the test tube hole sites of the amplification module are distributed in a staggered and crossed manner in a plurality of rows;
the optical detection head is a Y-shaped light guide optical fiber, one end of the optical detection head detects a test tube hole site, the other end of the optical detection head is bifurcated into two parts, one part is connected with a light source, and the other part is connected with a photoelectric induction element;
the optical filtering module comprises an excitation light lens, an excitation light filter, a detection filter and a detection lens;
the moving mechanism comprises a stepping motor, a sliding guide rail, a screw rod, a sliding block and a screw rod nut;
the signal processing and analyzing module is used for amplifying the fluorescence signal and analyzing and processing the fluorescence signal;
a control module for controlling the movement of the movement mechanism;
preferably, the amplification module is provided with a fluorescence detection hole at the side of each test tube reaction hole.
Preferably, a light guide element is arranged in the fluorescence detection hole.
Preferably, a module cover for isolating the reaction module from heat conduction with the environment is arranged above the amplification module.
Preferably, an optical fixing seat for fixing the optical detection head is arranged on the movement mechanism.
Preferably, a photoelectric detection element for optical signals is arranged in the signal processing and analyzing module.
Preferably, an excitation light source is arranged in the signal processing and analyzing module.
Preferably, a shielding cover for shielding the environmental noise interference is arranged outside the signal processing and analyzing module.
Preferably, the two sides of the optical fixing seat are provided with optical coupling shading sheets for shading an optical coupler.
Compared with the prior art, the gain effect of the invention is as follows:
the number of nucleic acid samples which can be detected within the same scanning distance is doubled, thereby reducing the detection time of the whole experiment;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the equipment cost is greatly reduced;
when the nucleic acid amplification detection scheme with the same number of reaction holes is adopted, the volume of the equipment is greatly reduced;
during detection, the equipment cost and the equipment volume are greatly optimized, so that the application range of the detection equipment is expanded.
Drawings
FIG. 1 is a perspective view of the overall structure of a novel optical detection system for nucleic acid amplification.
FIG. 2 is a side view of the overall structure of the novel optical detection system for nucleic acid amplification.
FIG. 3 is a perspective view showing the overall structure of the novel optical detection system for nucleic acid amplification, with the control part (6) hidden so as to view the lower structure.
FIG. 4 is a cross-sectional view of an amplification module.
FIG. 5 is a three-dimensional view of an amplification module.
Fig. 6 is a perspective view of the optical detection head, the optical filtering module, and the signal processing and analyzing module assembly.
Fig. 7 is a sectional view of the optical detection head, the optical filter module, and the signal processing and analyzing module assembly.
Fig. 8 is a schematic diagram of optical signal transmission.
Fig. 9 is a front view and a bottom view of the motion mechanism.
Detailed Description
The technical embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the present invention.
As shown in FIGS. 1 and 3, the present invention discloses a novel optical detection system for nucleic acid amplification, comprising: the device comprises an amplification module (1), an optical detection head (2), a movement mechanism (3), a right side optical coupler (7), an optical filtering module (4), a signal processing and analyzing module (5), a control module (6), a left side optical coupler (10), a right side light shielding sheet (8) and a left side light shielding sheet (9). Wherein:
the amplification module (1) is provided with test tube hole sites for holding reaction test tubes, the test tube hole sites are staggered in two rows as shown in figure 5, a detection hole (15) is arranged in each test tube hole, and the amplification module controls the temperature of the test tubes to realize nucleic acid amplification reaction. As shown in FIG. 4, the amplification module (1) is provided with a module cover (11), a metal reaction block (12), a light guide rod (13), and a lens (14).
The optical detection head (2) is a Y-shaped optical fiber as shown in fig. 6, one end of the optical detection head is used for detecting a fluorescent signal in the hole of the reaction module (1), the other end of the optical detection head is divided into two parts, one part is connected with an excitation light source, and the other part is connected with a photoelectric detection element.
The movement mechanism (3), as shown in fig. 9, includes: the device comprises a stepping motor (27), a screw rod (28), a linear guide rail (29) and a sliding block (30), wherein a moving mechanism (3) is used for driving an optical detection head (2), an optical filtering module (4) and a signal processing and analyzing module (5) to perform back and forth linear scanning movement, and a fluorescence signal of an amplification module (1) is detected.
The optical filter module (4), as shown in fig. 7, includes: the device comprises an optical fixing seat (17), an excitation light filter (18), an excitation light lens (19), a detection filter (21) and a detection lens (22), wherein the optical filtering module (4) is used for filtering useless excitation signals and detection signals.
The signal processing and analyzing module (5), as shown in fig. 7, includes: the device comprises a light source (20), a photoelectric detection element (23), a signal processing analysis circuit board (24) and a shielding cover (25).
And the control module (6) is used for controlling the motion mechanism (3) to perform back and forth scanning motion.
The flow of the novel optical detection system for nucleic acid amplification is described in further detail below with reference to FIG. 3.
Novel nucleic acid amplification optical detection system starts, control module (6) signal control motion (3) begin linear motion left, motion (3) drive optical detection head (2), optical filtering module (4), signal processing analysis module (5) move together, left side light-shielding piece (9) when motion (3) move to the leftmost position left side light-shielding piece (10) send position signal to control module (6) when inserting left side opto-coupler (10) inslot, control module (6) control motion (3) reverse motion, optical detection head (2) begin to carry out the first row to the test tube hole site in reaction module (1), the hole detection is pursued in the second row alternately, the single scanning can finish with the whole detections of the fluorescence signal of two rows. When the movement mechanism (3) moves to the rightmost position in the figure 3, the right side light shielding piece (8) is inserted into the right side optical coupler (7) groove, the right side optical coupler (7) sends a position signal to the control module (6), and the control module (6) controls the movement mechanism (3) to change the direction and return to the original position for next detection.
Now, the optical signaling process of the information nucleic acid amplification optical detection system will be described in further detail with reference to FIG. 8. In the scanning and detecting process of the novel nucleic acid amplification optical detection system, a light source (20) is lightened, exciting light is focused through an exciting light lens (19) and then filtered through an exciting light optical filter (18), the light passes through an exciting light interface of an optical detection head (16) and then irradiates a reaction test tube (27) in a test tube hole (26), a reaction system in the reaction test tube (27) emits light with other wavelength after receiving the exciting light, the emitted light is transmitted to the detection interface through the detection head (16), the light is filtered through a detection optical filter (21) and focused through a detection lens (22), finally, an optical signal is converted into an electric signal through a photoelectric detection element (23), and the electric signal is analyzed and processed through a signal processing analysis circuit board (24).
It is to be understood that: although the present invention has been described in detail, the present invention is only for the purpose of illustration and not limitation, and any invention that does not depart from the spirit of the present invention falls within the scope of the present invention.
Claims (10)
1. A novel optical detection system for nucleic acid amplification, comprising:
the test tube hole sites of the amplification module are distributed in a staggered and crossed manner in a plurality of rows;
the optical detection head is a Y-shaped light guide optical fiber, one end of the optical detection head detects a test tube hole site, the other end of the optical detection head is bifurcated into two parts, one part is connected with a light source, and the other part is connected with a photoelectric induction element;
the optical filtering module comprises an excitation light lens, an excitation light filter, a detection filter and a detection lens;
the moving mechanism comprises a stepping motor, a sliding guide rail, a screw rod, a sliding block and a screw rod nut;
the signal processing and analyzing module is used for amplifying the fluorescence signal and analyzing and processing the fluorescence signal;
a control module, the control component for controlling movement of the movement mechanism.
2. The novel optical detection system for nucleic acid amplification of claim 1, wherein the amplification module is provided with a fluorescence detection hole at the side of each reaction hole of the test tube.
3. The novel optical detection system for nucleic acid amplification of claim 2, wherein a light guide element is disposed in the fluorescence detection well.
4. The optical detection system for nucleic acid amplification of claim 1, wherein a module cover for isolating the reaction module from heat conduction with the environment is disposed above the amplification module.
5. The system of claim 1, wherein the moving mechanism is provided with an optical holder for fixing the optical detection head.
6. The optical detection system for nucleic acid amplification according to claim 1, wherein the right and left sides of the moving mechanism are provided with optical couplers for detecting two-sided position signals.
7. The optical detection system for nucleic acid amplification according to claim 1, wherein a shielding cover for shielding environmental noise is disposed outside the signal processing and analyzing module.
8. The optical detection system for nucleic acid amplification of claim 1, wherein a photodetector for optical signal is disposed in the signal processing and analyzing module.
9. The system according to claim 1, wherein an excitation light source is disposed in the signal processing and analyzing module.
10. The system of claim 4, wherein the optical fixing base is provided with two light-shielding sheets for shielding the light-coupler.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010480238.4A CN112442444A (en) | 2020-05-30 | 2020-05-30 | Novel nucleic acid amplification optical detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010480238.4A CN112442444A (en) | 2020-05-30 | 2020-05-30 | Novel nucleic acid amplification optical detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112442444A true CN112442444A (en) | 2021-03-05 |
Family
ID=74733305
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010480238.4A Pending CN112442444A (en) | 2020-05-30 | 2020-05-30 | Novel nucleic acid amplification optical detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112442444A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092433A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Fluorescent detection device with oscillation |
-
2020
- 2020-05-30 CN CN202010480238.4A patent/CN112442444A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113092433A (en) * | 2021-04-09 | 2021-07-09 | 广州新成生物科技有限公司 | Fluorescent detection device with oscillation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103901189B (en) | Immune detection micro-fluidic chip fluorescent quantitation automatic detection device | |
| CN106010954B (en) | Novel droplet type digital pcr Systems for optical inspection, device and method | |
| CN212293591U (en) | Novel nucleic acid amplification optical detection system | |
| CN102243165B (en) | Photonic crystal coded microsphere biochip detection device | |
| CN112442444A (en) | Novel nucleic acid amplification optical detection system | |
| Lu et al. | Time-gated orthogonal scanning automated microscopy (OSAM) for high-speed cell detection and analysis | |
| CN202110128U (en) | Photonic crystal coding microsphere biochip detection device | |
| CN211471428U (en) | Fluorescence scanning system for PCR instrument | |
| CN110935498A (en) | Fluorescence scanning system for PCR instrument | |
| CN211263193U (en) | Optical analysis device for detecting strip-shaped fluorescent signal | |
| US20230330664A1 (en) | Three-dimensional sample scanning system | |
| US10605657B2 (en) | Scanner photometer and methods | |
| CN216808854U (en) | Nucleic acid extraction PCR amplification all-in-one | |
| CN212483358U (en) | Nucleic acid optical detection system based on top scanning detection | |
| EP2344852B1 (en) | Scanner photometer head and associated method | |
| CN111592982A (en) | Novel portable nucleic acid amplification analyzer | |
| CN114062326A (en) | Nucleic acid optical detection system based on top scanning detection | |
| CN212483359U (en) | Multi-channel detection system for real-time nucleic acid fluorescence detection | |
| CN210269617U (en) | Optical detection device and capillary electrophoresis apparatus | |
| CN212025347U (en) | Fluorescence detection device | |
| CN202770771U (en) | Fluorescence quantitative detection system based on ectopic multi-wavelength detection | |
| CN103115901B (en) | Device for detecting biological chips based on resonance light scattering | |
| CN111189814B (en) | Fluorescence detection device based on micro-fluidic chip and micro-fluidic chip detection system | |
| CN220490664U (en) | Linear light source excitation device for fluorescence detection | |
| CN216837964U (en) | Nucleic acid analysis optical scanning device, full-automatic nucleic acid analysis detection device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |