CN112425509A - Sterile culture medium for waxy phalaenopsis industrial tissue culture - Google Patents
Sterile culture medium for waxy phalaenopsis industrial tissue culture Download PDFInfo
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- CN112425509A CN112425509A CN202011490729.3A CN202011490729A CN112425509A CN 112425509 A CN112425509 A CN 112425509A CN 202011490729 A CN202011490729 A CN 202011490729A CN 112425509 A CN112425509 A CN 112425509A
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- fixedly connected
- culture medium
- buffer
- sterile
- motor
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- 239000001963 growth medium Substances 0.000 title claims abstract description 62
- 241001505935 Phalaenopsis Species 0.000 title claims abstract description 22
- 230000005540 biological transmission Effects 0.000 claims abstract description 48
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 230000003139 buffering effect Effects 0.000 claims abstract description 5
- 230000008878 coupling Effects 0.000 claims description 10
- 238000010168 coupling process Methods 0.000 claims description 10
- 238000005859 coupling reaction Methods 0.000 claims description 10
- 244000127818 Phalaenopsis amabilis Species 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000010923 batch production Methods 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 11
- 238000011282 treatment Methods 0.000 abstract description 8
- 238000005286 illumination Methods 0.000 abstract description 2
- 230000002070 germicidal effect Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sterile culture medium for waxy phalaenopsis industrial tissue culture, which comprises a base, a transmission mechanism, a driving assembly, a buffering assembly and a locking assembly, wherein a sterile box is fixedly arranged on one side of the top surface of the base; a sliding plate is arranged at the top between the T-shaped guide rails, a motor box is arranged on the top surface of the sliding plate, and rotating columns are fixedly connected in inner rings of the first bearings; a culture medium is arranged in each guide pipe; the transmission mechanism is arranged on the bottom surface of the sliding plate; the driving assembly is arranged in the driving cavity; in the invention, the driving component is matched with the transmission mechanism for use, so that the sliding plate can conveniently transversely move towards the same direction on the T-shaped guide rail; the problem that the existing culture medium is inconvenient to move and move transversely when being sterilized is solved; the waxy phalaenopsis seeds in the culture medium are conveniently sterilized by the illumination of the sterilizing lamp through the rotation adjustment of the mounting plate; solves the problem that the existing culture medium is easy to have the culture medium shading from top to bottom when the sterilization treatment is carried out.
Description
Technical Field
The invention relates to the technical field of sterile culture of waxy phalaenopsis, in particular to a sterile culture medium for industrial tissue culture of waxy phalaenopsis.
Background
In the prior art, when waxy phalaenopsis is cultured, the waxy phalaenopsis is usually cultured by a culture medium, and when the waxy phalaenopsis is cultured, the culture medium needs to be sterilized so as to be convenient for long-time storage; at present, sterile culture media are often preserved by adopting a sterilization treatment and then transferring to a storage device, but the prior culture media have the following problems during the sterilization treatment: (1) the existing culture medium is inconvenient for carrying out a plurality of sterilization treatments together and simultaneously placing a plurality of culture mediums into a sterilization box for sterilization operation, so that the labor amount of workers is increased, and the sterilization efficiency of the culture medium is influenced; (2) when the existing culture medium is sterilized, the defect that shading occurs at the upper and lower parts easily exists, so that effective sterilization treatment cannot be carried out on each culture medium; therefore, the above problems need to be solved optimally.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a sterile culture medium for industrial tissue culture of waxy phalaenopsis.
In order to achieve the purpose, the invention adopts the following technical scheme: the sterile culture medium for the waxy butterfly orchid industrial tissue culture comprises a base, a transmission mechanism, a driving assembly, a buffering assembly and a locking assembly, wherein a sterile box is fixedly arranged on one side of the top surface of the base, a T-shaped guide rail is transversely and fixedly connected to the front end and the rear end of the top surface of the other side of the base, and one end of the T-shaped guide rail penetrates through and extends into the sterile box; a sliding plate is horizontally arranged at the top between the T-shaped guide rails, and the front end and the rear end of the top surface of the sliding plate are both transversely provided with T-shaped sliding chutes in a matched manner; the top surface of the sliding plate is provided with a motor box, a first bearing is fixedly connected to the top surface in the motor box, rotating columns are vertically and fixedly connected to inner rings of the first bearing, sleeves are sleeved on columns of the rotating columns at equal intervals, mounting plates are transversely and fixedly connected to two sides of each sleeve, circular openings are formed in the middle of the top surface of each mounting plate at equal intervals, guide pipes are vertically and fixedly connected to the inside of each circular opening, and a culture medium is vertically arranged in each guide pipe; the transmission mechanism is arranged on the bottom surface of the sliding plate; the middle part in the sliding plate is provided with a driving cavity, and the driving component is arranged in the driving cavity.
Preferably, a humidifier is fixedly arranged on one side of the sterile box, and a plurality of sterilizing lamps are transversely and fixedly connected to the inner top surface of the sterile box at equal intervals; a pipeline hole is transversely formed in the upper part of one side in the sterile box, a bent pipe-shaped connecting pipe is transversely arranged in the pipeline hole, the output end of the humidifier at the outer end of the connecting pipe is fixedly connected, and a spray head is vertically and fixedly arranged at the inner end, located in the box body, of the connecting pipe; the other side of the sterile box is provided with a rectangular opening, and the upper part of the other side of the sterile box is movably hinged with a closed door through a hinge matched with the rectangular opening; the fixed cover of lower part level of rotating the post is equipped with the disc, a plurality of mounting holes have been seted up to the top surface equidistance of disc, every all be fixed in the mounting hole and be equipped with the fan.
Preferably, an end cover is horizontally and fixedly connected to the top of each culture medium, and an annular supporting plate is horizontally arranged below the bottom surface of each end cover; every the cushion chamber of a plurality of rectangles, every are seted up to equal equidistance in the edge week side in the mounting panel all be equipped with the buffering subassembly in the cushion chamber.
Preferably, the buffer assembly comprises guide rods, buffer plates, buffer springs and buffer rods, wherein the guide rods are vertically and fixedly connected to two sides in each buffer cavity, the buffer plates are horizontally sleeved between the guide rods, guide holes are vertically formed in two sides of the top surface of each buffer plate, the buffer plates are movably sleeved on the guide rods through the guide holes, the buffer springs are movably sleeved on the guide rod bodies below the buffer plates, and two ends of each buffer spring are fixedly connected with the bottom surfaces of the buffer plates and the inner bottom surfaces of the buffer cavities respectively; every the equal vertical rigid coupling in top surface middle part of buffer board has the buffer beam, and every the top of buffer beam all moves about and runs through outside the cushion chamber and with the bottom surface rigid coupling of annular layer board.
Preferably, the transmission mechanism comprises a rotating shaft, a driven gear, a fixed rack, a transmission shaft and a driving gear, wherein the front end and the rear end of the bottom surface of the sliding plate are fixedly connected with second bearings, the inner ring of each second bearing is vertically and fixedly connected with the rotating shaft, a driven gear is horizontally and fixedly sleeved on the shaft body of each rotating shaft, the inner side surface of each T-shaped guide rail is transversely and fixedly connected with the fixed rack, and external teeth of each driven gear are meshed with the fixed rack for transmission; a third bearing is fixedly connected to the middle of the bottom surface of the sliding plate, a transmission shaft is vertically and fixedly connected to the inner ring of the third bearing, and a driving gear is horizontally sleeved on the shaft body of the transmission shaft; and the teeth at the front end and the rear end of the driving gear are respectively in meshed transmission with the front driven gear and the rear driven gear.
Preferably, drive assembly includes the second motor, takes the initiative awl tooth, driven awl tooth, the second motor vertically sets up in the motor intracavity, the coaxial rigid coupling in motor shaft rear end of second motor has the initiative awl tooth, the top activity of transmission shaft is run through into the motor intracavity, just the horizontal rigid coupling in top of transmission shaft has driven awl tooth, take the initiative awl tooth and driven awl tooth meshing transmission.
Preferably, a first motor is vertically arranged in the middle of the motor box, the bottom end of the rotating column penetrates into the motor box, and the bottom end of the rotating column is coaxially and fixedly connected with a motor shaft of the first motor through a coupler; and storage batteries are arranged on two sides in the motor box.
Preferably, the top and the bottom in each sleeve are horizontally and fixedly connected with a fourth bearing, and the inner ring of each fourth bearing is fixedly connected with the cylinder of the rotating column; a circle of limiting groove is formed in the rotating column body in the middle of each sleeve, an arc-shaped limiting plate is transversely arranged at the front end in each limiting groove, and a fifth bearing is vertically and fixedly connected to the front end of the outer arc surface of each limiting plate; each threaded hole is longitudinally formed in the middle of the front end face of each sleeve, each threaded hole is longitudinally and spirally connected with a locking bolt, and the inner end of each locking bolt is fixedly connected into the inner ring of the fifth bearing.
Compared with the prior art, the invention has the beneficial effects that: in the present invention, in the case of the present invention,
1. the driving assembly is convenient for driving the transmission mechanism to rotate, and the two driven gears of the transmission mechanism are meshed with the fixed rack for transmission, so that the sliding plate can move transversely on the T-shaped guide rail in the same direction through the opposite rotation of the two driven gears; the culture medium placed in the sterile box can be conveniently and transversely moved into the sterile box through the transverse movement of the sliding plate, so that the stability of the sliding plate during transverse movement can be effectively improved, and the workload of workers can be effectively reduced; the culture medium placed in the sterile box can be conveniently and automatically moved transversely, so that the problem that the existing culture medium is inconvenient to move transversely when being sterilized is solved;
2. the mounting plate is horizontally rotated and adjusted, so that the culture medium placed on the mounting plate can be horizontally and angularly adjusted, the culture medium on each layer of the rotating column can be vertically staggered, and waxy phalaenopsis seeds in each culture medium can be uniformly sterilized by the illumination of a sterilizing lamp; the situation that the upper culture medium is easy to shade the lower culture medium is effectively reduced; the buffer assembly is convenient for effectively reducing the impact force generated when the culture medium is placed and the shaking generated during the transverse movement, and is convenient for improving the stability of the culture medium during the transverse movement; solves the problem that the existing culture medium is easy to have the culture medium shading from top to bottom when the sterilization treatment is carried out.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without limiting the invention. In the drawings:
FIG. 1 is a schematic cross-sectional front view of the present invention;
FIG. 2 is a schematic cross-sectional view of the mounting plate of the present invention;
FIG. 3 is a schematic side sectional view of the sliding plate of the present invention;
FIG. 4 is a schematic top view of the transmission mechanism of the present invention;
FIG. 5 is a cross-sectional view of the sleeve of the present invention;
FIG. 6 is a schematic top sectional view of the sleeve of the present invention;
number in the figure: 1. a base; 2. a sterile box; 3. a T-shaped guide rail; 4. a sliding plate; 5. a motor case; 6. rotating the column; 7. a sleeve; 8. mounting a plate; 9. a guide tube; 10. a culture medium; 11. a humidifier; 12. a germicidal lamp; 13. a closing door; 14. an end cap; 15. an annular pallet; 16. a guide bar; 17. a buffer plate; 18. a buffer spring; 19. a buffer rod; 20. a rotating shaft; 21. a driven gear; 22. fixing a rack; 23. a drive shaft; 24. a driving gear; 25. a first motor; 26. a second motor; 27. driving bevel gears; 28. driven bevel gears; 29. a limiting plate; 30. locking the bolt; 31. a connecting pipe; 32. a spray head; 33. a fan.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1: referring to fig. 1-6, the sterile culture medium for waxy butterfly orchid industrial tissue culture comprises a base 1, a transmission mechanism, a driving assembly, a buffering assembly and a locking assembly, wherein the base 1 is in a horizontally arranged rectangular plate shape, a sterile box 2 is fixedly arranged on one side of the top surface of the base 1, a T-shaped guide rail 3 is transversely and fixedly connected to the front end and the rear end of the top surface of the other side of the base 1, and one end of the T-shaped guide rail 3 extends into the sterile box 2 in a penetrating manner; a sliding plate 4 is horizontally arranged at the top between the T-shaped guide rails 3, and the front end and the rear end of the top surface of the sliding plate 4 are both transversely provided with T-shaped sliding chutes in a matched manner; the top surface of the sliding plate 4 is provided with a motor box 5, the top surface in the motor box 5 is fixedly connected with a first bearing, rotating columns 6 are vertically and fixedly connected in inner rings of the first bearing, sleeves 7 are equidistantly sleeved on the cylinders of the rotating columns 6, mounting plates 8 are transversely and fixedly connected on two sides of each sleeve 7, circular openings are equidistantly formed in the middle of the top surface of each mounting plate 8, guide pipes 9 are vertically and fixedly connected in the circular openings, and a culture medium 10 is vertically arranged in each guide pipe 9; the transmission mechanism is arranged on the bottom surface of the sliding plate 4; the middle part in the sliding plate 4 is provided with a driving cavity, and the driving component is arranged in the driving cavity.
In the invention, a humidifier 11 is fixedly arranged on one side of the sterile box 2, the model of the humidifier 11 is YKX-3.0B, and a plurality of sterilizing lamps 12 are transversely fixedly connected with the inner top surface of the sterile box 2 at equal intervals; the model of the sterilizing lamp 12 is ES-UVZJ, the upper part of one side in the sterile box 2 is transversely provided with a pipeline hole, a bent pipe-shaped connecting pipe 31 is transversely arranged in the pipeline hole, the outer end of the connecting pipe 31 is fixedly connected with the output end of the humidifier 11, and the inner end of the connecting pipe 31 in the box body is vertically and fixedly provided with a spray head 32; the other side of the sterile box 2 is provided with a rectangular opening, and the upper part of the other side of the sterile box 2 is movably hinged with a sealing door 13 through a hinge matched with the rectangular opening; a disc is horizontally fixedly sleeved at the lower part of the rotating column 6, a plurality of mounting holes are formed in the top surface of the disc at equal intervals, a fan 33 is fixedly arranged in each mounting hole, and the model of the fan 33 is DC 3004; carry out germicidal treatment through opening sterilamp 12 to the waxy moth orchid seed of cultivateing in box inside and culture medium 10, carry out humidification treatment in to aseptic box 2 through connecting pipe 31 and shower nozzle 32 through control humidifier 11, fan 33 on the rethread control disc is convenient for make the air current in aseptic box 2 carry out the circulation flow, is convenient for accelerate the work efficiency to the humidification in the box, is convenient for improve the homogeneity to aseptic box 2 interior humidification.
In the invention, the top of each culture medium 10 is horizontally and fixedly connected with an end cover 14, and an annular supporting plate 15 is horizontally arranged below the bottom surface of each end cover 14; a plurality of rectangular buffer cavities are arranged on the periphery of the edge in each mounting plate 8 at equal intervals, and each buffer cavity is internally provided with a buffer assembly; the buffer assembly comprises guide rods 16, buffer plates 17, buffer springs 18 and buffer rods 19, wherein the guide rods 16 are vertically fixedly connected to two sides in each buffer cavity, the buffer plates 17 are horizontally sleeved between the guide rods 16, guide holes are vertically formed in two sides of the top surface of each buffer plate 17, the buffer plates 17 are movably sleeved on the guide rods 16 through the guide holes, the buffer springs 18 are movably sleeved on rod bodies of the guide rods 16 below each buffer plate 17, and two ends of each buffer spring 18 are fixedly connected with the bottom surfaces of the buffer plates 17 and the inner bottom surfaces of the buffer cavities respectively; the equal vertical rigid coupling in top surface middle part of every buffer board 17 has buffer beam 19, and the top of every buffer beam 19 all activity run through outside the cushion chamber and with the bottom surface rigid coupling of annular layer board 15.
In the invention, the transmission mechanism comprises a rotating shaft 20, a driven gear 21, a fixed rack 22, a transmission shaft 23 and a driving gear 24, wherein the front end and the rear end of the bottom surface of the sliding plate 4 are fixedly connected with second bearings, the inner ring of each second bearing is vertically and fixedly connected with the rotating shaft 20, the shaft body of each rotating shaft 20 is horizontally and fixedly sleeved with the driven gear 21, the inner side surface of each T-shaped guide rail 3 is transversely and fixedly connected with the fixed rack 22, and external teeth of each driven gear 21 are in meshing transmission with the fixed rack 22; a third bearing is fixedly connected to the middle of the bottom surface of the sliding plate 4, a transmission shaft 23 is vertically and fixedly connected to the inner ring of the third bearing, and a driving gear 24 is horizontally sleeved on the shaft body of the transmission shaft 23; the teeth at the front and rear ends of the driving gear 24 are respectively engaged with the front and rear driven gears 21 for transmission.
In the invention, the driving component comprises a second motor 26, a driving bevel gear 27 and a driven bevel gear 28, the model of the second motor 26 is YE2-112M-4, the second motor 26 is longitudinally arranged in a motor cavity, the rear end of a motor shaft of the second motor 26 is coaxially and fixedly connected with the driving bevel gear 27, the top end of the transmission shaft 23 movably penetrates into the motor cavity, the top end of the transmission shaft 23 is horizontally and fixedly connected with the driven bevel gear 28, and the driving bevel gear 27 is in meshing transmission with the driven bevel gear 28; the middle part in the motor box 5 is vertically provided with a first motor 25, the model of the first motor 25 is YE2-112M-4, the bottom end of the rotating column 6 penetrates into the motor box 5, and the bottom end of the rotating column 6 is coaxially and fixedly connected with a motor shaft of the first motor 25 through a coupler; the two sides in the motor box 5 are provided with storage batteries; the driving assembly is convenient for driving the transmission mechanism to rotate, and the two driven gears 21 of the transmission mechanism are meshed with the fixed rack 22 for transmission, so that the two driven gears 21 rotate in opposite directions to enable the sliding plate to transversely move on the T-shaped guide rail in the same direction; the culture medium placed is convenient to move into the sterile box 2 through the lateral movement of the sliding plate.
In the invention, the top and the bottom in each sleeve 7 are horizontally and fixedly connected with a fourth bearing, and the inner ring of each fourth bearing is fixedly connected with the cylinder of the rotating column 6; a circle of limiting groove is formed in the cylinder body of the rotating column 6 in the middle of each sleeve 7, an arc-shaped limiting plate 29 is transversely arranged at the front end in each limiting groove, and a fifth bearing is vertically and fixedly connected to the front end of the outer arc surface of each limiting plate 29; threaded holes are longitudinally formed in the middle of the front end face of each sleeve 7, locking bolts 30 are longitudinally and spirally connected in the threaded holes, and the inner ends of the locking bolts 30 are fixedly connected in the inner ring of the fifth bearing.
Example 2: in this embodiment, the present invention further provides an operating method of a sterile culture medium for industrial tissue culture of waxy phalaenopsis, including the following steps:
firstly, electrically connecting the humidifier 11 and the germicidal lamp 12 with an external power supply through leads, and then electrically connecting the first motor 25, the second motor 26 and the fan 33 with a storage battery through leads respectively; the culture mediums 10 which are workers and placed with waxy phalaenopsis seeds are respectively placed in the guide tubes 9, and an end cover 14 at the top of each culture medium 10 is pressed at the top of the annular supporting plate 15;
step two, the end cover 14 is pressed on the top of the annular supporting plate 15, so that the buffer rod 19 is pressed and lowered conveniently, the buffer plate 17 is pushed to lift and slide on the rod body of the guide rod 16 by the downward pressing of the buffer rod 19, the buffer spring 18 is pressed by the upward and downward pressing of the buffer plate 17, the impact force generated by the downward falling of the buffer plate 17 is reduced conveniently by the elastic action of the buffer spring 18, the end cover 14 pressed on the top of the annular supporting plate 15 is buffered conveniently, and the buffer protection effect is effectively realized when the culture medium 10 is placed;
step three, when the placed culture medium 10 needs to be horizontally adjusted, the sleeve 7 is conveniently driven to horizontally rotate on the column body of the rotating column 6 by rotating the mounting plate 8, so that the culture medium 10 on each layer of the rotating column 6 is conveniently vertically staggered, and the waxy phalaenopsis seeds in each culture medium 10 are conveniently sterilized by the light of the sterilizing lamp 12 uniformly; the locking bolt 30 is rotated inwards to push the limiting plate 29 to be pushed towards the limiting groove on the rotating column 6, so that the inner arc surface of the limiting plate 29 is locked in the limiting groove of the rotating column 6; the horizontal position of the adjusted culture medium 10 is convenient to limit and fix;
step four, when the mounted culture medium 10 needs to be moved into the sterile box 2, the closing door 13 is opened, the second motor 26 is controlled to drive the driving bevel gear 27 to rotate, the driving bevel gear 27 and the driven bevel gear 28 are in meshing transmission, the transmission shaft 23 is convenient to drive to rotate, the driving gear 24 is convenient to drive to rotate through the rotation of the transmission shaft 23, the two driven gears 25 are convenient to rotate in opposite directions through the meshing transmission of the driving gear 24 and the two driven gears 25, and the sliding plate 4 is convenient to move transversely on the T-shaped guide rail 3 in the same direction through the meshing transmission of the two driven gears 25 and the fixed rack 22; the transverse movement of the sliding plate 4 is convenient to drive the rotating column 6 arranged at the top of the motor box 5 to transversely move, and the transverse movement of the rotating column 6 is convenient to drive the culture medium 10 arranged on the mounting plate 8 to transversely move into the sterile box 2;
step five, after the culture medium 10 is moved into the sterile box 2, the closing door is closed, the sterilization lamp 12 is turned on to sterilize the inside of the culture box body and waxy butterfly orchid seeds in the culture medium 10, then the humidifier 11 is controlled to humidify the sterile box 2 through the connecting pipe 31 and the spray head 32, and then the fan 33 on the control disc is used for facilitating the air flow in the sterile box 2 to circularly flow, so that the work efficiency of humidifying the inside of the box body is accelerated, and the uniformity of humidifying the inside of the sterile box 2 is improved.
Step six, the rotating column 6 is conveniently driven to rotate by controlling the first motor 25, the mounting plates 8 on the two sides of the sleeve 7 are conveniently driven to rotate by rotating the rotating column 6, the culture mediums 10 are conveniently driven to horizontally rotate in the sterile box 2 by rotating the mounting plates 8, and each culture medium 10 is conveniently and uniformly irradiated by the light of the sterilizing lamp 12 by horizontally rotating the culture mediums 10; so as to effectively improve the sterilization effect on the culture medium 10.
And seventhly, after the waxy phalaenopsis seeds are cultured, the driving assembly is controlled to drive the transmission mechanism, so that the sliding plate 4 can move out of the sterile box 2 conveniently, and then the power supply is cut off.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (8)
1. Waxy butterfly orchid batch production group banks up with aseptic culture medium with earth, including base (1), drive mechanism, drive assembly, buffer unit and locking Assembly, its characterized in that: a sterile box (2) is fixedly arranged on one side of the top surface of the base (1), T-shaped guide rails (3) are transversely and fixedly connected to the front end and the rear end of the top surface of the other side of the base (1), and one ends of the T-shaped guide rails (3) penetrate through and extend into the sterile box (2); a sliding plate (4) is horizontally arranged at the top between the T-shaped guide rails (3), and T-shaped sliding grooves are transversely formed in the front end and the rear end of the top surface of the sliding plate (4) in a matched mode; a motor box (5) is arranged on the top surface of the sliding plate (4), a first bearing is fixedly connected to the top surface in the motor box (5), rotating columns (6) are vertically and fixedly connected to inner rings of the first bearing, sleeves (7) are sleeved on columns of the rotating columns (6) at equal intervals, mounting plates (8) are transversely and fixedly connected to two sides of each sleeve (7), circular openings are formed in the middle of the top surface of each mounting plate (8) at equal intervals, guide pipes (9) are vertically and fixedly connected to the circular openings, and a culture medium (10) is vertically arranged in each guide pipe (9); the transmission mechanism is arranged on the bottom surface of the sliding plate (4); the middle part in the sliding plate (4) is provided with a driving cavity, and the driving component is arranged in the driving cavity.
2. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: a humidifier (11) is fixedly arranged on one side of the sterile box (2), and a plurality of sterilizing lamps (12) are transversely and fixedly connected with the inner top surface of the sterile box (2) at equal intervals; a pipeline hole is transversely formed in the upper portion of one side in the sterile box (2), a bent pipe-shaped connecting pipe (31) is transversely arranged in the pipeline hole, the output end of the humidifier (11) at the outer end of the connecting pipe (31) is fixedly connected, and a spray head (32) is vertically and fixedly arranged at the inner end, located in the box body, of the connecting pipe (31); a rectangular opening is formed in the other side of the sterile box (2), and a closed door (13) is movably hinged to the upper portion of the other side of the sterile box (2) through a hinge matched with the rectangular opening; the fixed cover of lower part level of rotating post (6) is equipped with the disc, a plurality of mounting holes have been seted up to the top surface equidistance of disc, every all fixed fan (33) that are equipped with in the mounting hole.
3. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: an end cover (14) is horizontally and fixedly connected to the top of each culture medium (10), and an annular supporting plate (15) is horizontally arranged below the bottom surface of each end cover (14); every the cushion chamber of a plurality of rectangles, every are seted up to equal equidistance in all sides of edge in mounting panel (8) all be equipped with the buffering subassembly in the cushion chamber.
4. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: the buffer assembly comprises guide rods (16), buffer plates (17), buffer springs (18) and buffer rods (19), wherein the guide rods (16) are vertically fixedly connected to two sides in each buffer cavity, the buffer plates (17) are horizontally sleeved between the guide rods (16), guide holes are vertically formed in two sides of the top surface of each buffer plate (17), the buffer plates (17) are movably sleeved on the guide rods (16) through the guide holes, the buffer springs (18) are movably sleeved on the rod bodies of the guide rods (16) below the buffer plates (17), and two ends of each buffer spring (18) are respectively fixedly connected with the bottom surfaces of the buffer plates (17) and the inner bottom surfaces of the buffer cavities; every the equal vertical rigid coupling in top surface middle part of buffer board (17) has buffer beam (19), and every the top of buffer beam (19) all moves about and runs through outside the cushion chamber and with the bottom surface rigid coupling of annular layer board (15).
5. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: the transmission mechanism comprises a rotating shaft (20), a driven gear (21), a fixed rack (22), a transmission shaft (23) and a driving gear (24), wherein the front end and the rear end of the bottom surface of the sliding plate (4) are fixedly connected with second bearings, the inner ring of each second bearing is vertically and fixedly connected with the rotating shaft (20), the shaft body of each rotating shaft (20) is horizontally and fixedly sleeved with the driven gear (21), the inner side surface of each T-shaped guide rail (3) is transversely and fixedly connected with the fixed rack (22), and external teeth of each driven gear (21) are in meshing transmission with the fixed rack (22); a third bearing is fixedly connected to the middle of the bottom surface of the sliding plate (4), a transmission shaft (23) is vertically and fixedly connected to the inner ring of the third bearing, and a driving gear (24) is horizontally sleeved on the shaft body of the transmission shaft (23); the teeth at the front end and the rear end of the driving gear (24) are respectively in meshed transmission with the front driven gear and the rear driven gear (21).
6. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 5, which is characterized in that: drive assembly includes second motor (26), initiative awl tooth (27), driven awl tooth (28), second motor (26) vertically sets up in the motor cavity, the coaxial rigid coupling in motor shaft rear end of second motor (26) has initiative awl tooth (27), the top activity of transmission shaft (23) is run through into the motor cavity, just the horizontal rigid coupling in top of transmission shaft (23) has driven awl tooth (28), initiative awl tooth (27) and driven awl tooth (28) meshing transmission.
7. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: a first motor (25) is vertically arranged in the middle of the motor box (5), the bottom end of the rotating column (6) penetrates into the motor box (5), and the bottom end of the rotating column (6) is coaxially and fixedly connected with a motor shaft of the first motor (25) through a coupler; and storage batteries are arranged on two sides in the motor box (5).
8. The sterile culture medium for the industrial tissue culture of the waxy phalaenopsis according to claim 1, which is characterized in that: the top and the bottom in each sleeve (7) are horizontally and fixedly connected with a fourth bearing, and the inner ring of each fourth bearing is fixedly connected with the cylinder of the rotating column (6); a circle of limiting groove is formed in the cylinder body of the rotating column (6) in the middle of each sleeve (7), an arc-shaped limiting plate (29) is transversely arranged at the front end in each limiting groove, and a fifth bearing is vertically and fixedly connected to the front end of the outer arc surface of each limiting plate (29); threaded holes are longitudinally formed in the middle of the front end face of each sleeve (7), each threaded hole is internally and longitudinally spirally connected with a locking bolt (30), and the inner end of each locking bolt (30) is fixedly connected into an inner ring of a fifth bearing.
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| CN202011490729.3A CN112425509A (en) | 2020-12-17 | 2020-12-17 | Sterile culture medium for waxy phalaenopsis industrial tissue culture |
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