CN112414826A - Smear dyeing machine - Google Patents
Smear dyeing machine Download PDFInfo
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- CN112414826A CN112414826A CN202011400879.0A CN202011400879A CN112414826A CN 112414826 A CN112414826 A CN 112414826A CN 202011400879 A CN202011400879 A CN 202011400879A CN 112414826 A CN112414826 A CN 112414826A
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- dyeing
- box
- smear
- waste liquid
- supply system
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- 238000004043 dyeing Methods 0.000 title claims abstract description 214
- 239000007788 liquid Substances 0.000 claims abstract description 86
- 239000002699 waste material Substances 0.000 claims abstract description 36
- 238000011084 recovery Methods 0.000 claims abstract description 30
- 238000010186 staining Methods 0.000 claims description 13
- 239000012192 staining solution Substances 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 7
- 239000011521 glass Substances 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 6
- 239000003085 diluting agent Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 239000011550 stock solution Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000015073 liquid stocks Nutrition 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
- G01N1/312—Apparatus therefor for samples mounted on planar substrates
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Fiber Materials (AREA)
Abstract
The invention discloses a smear dyeing machine which comprises a shell, a dyeing liquid supply system, a waste liquid recovery system and a plurality of dyeing boxes, wherein the dyeing liquid supply system, the waste liquid recovery system and the plurality of dyeing boxes are arranged in the shell, a platform used for supporting a smear is arranged in each dyeing box, the plurality of dyeing boxes are communicated with the dyeing liquid supply system and the waste liquid recovery system, the dyeing liquid supply system is used for conveying dyeing liquid to the dyeing boxes, and the waste liquid recovery system is used for recovering the dyeing liquid in the dyeing boxes. The slide glass of dry cell smear is placed on the platform of dyeing box in the working process, and dyeing liquor supply system carries the dyeing liquor to the dyeing box after the dyeing box is closed, then the waste liquid recovery system starts to retrieve the dyeing liquor in the dyeing box, takes out the smear that dyeing was accomplished. Realize mechanical intelligent dyeing through equipment, replace artifical dyeing, promote work efficiency, dyeing concentration and dyeing time accurate control promote the dyeing quality, and then promote the cell identification rate of accuracy.
Description
Technical Field
The invention relates to the field of detection equipment, in particular to a smear dyeing machine.
Background
A large number of medical detection cells need to be dyed, for example, marrow cells and the like can be dyed by Swiss, but in the prior art, the Swiss dyeing can only be used for drop dyeing and can not be used for dip dyeing, and large-scale dyeing has difficulty. And only manual dyeing can be carried out, mechanical intelligent dyeing cannot be realized, and the working efficiency is low. Meanwhile, the quality of the Ruhrstan's dyeing is closely related to the dyeing time, the concentration of the dye solution and the temperature and humidity of the environment, if the temperature is reduced in winter, the dyeing time is properly prolonged when the concentration of the dye solution is unchanged, and if the temperature is increased in summer, the three factors are opposite to each other, the three factors influence each other, the traditional manual dyeing process is very difficult to master, the dyeing concentration and the dyeing time are low in accuracy, the dyeing quality is difficult to control, and the cell identification accuracy is poor.
Therefore, how to provide a smear-dyeing machine that overcomes the above-mentioned problems is a technical problem that those skilled in the art need to solve at present.
Disclosure of Invention
The invention aims to provide a smear dyeing machine which realizes mechanical intelligent dyeing through equipment, improves the working efficiency, has accurate dyeing concentration and dyeing time, improves the dyeing quality and has high cell identification accuracy.
In order to solve the technical problem, the invention provides a smear dyeing machine, which comprises a shell, and a dyeing liquid supply system, a waste liquid recovery system and a plurality of dyeing boxes which are arranged in the shell, wherein a platform for supporting a smear is arranged in each dyeing box, the plurality of dyeing boxes are communicated with the dyeing liquid supply system and the waste liquid recovery system, the dyeing liquid supply system is used for conveying dyeing liquid to the dyeing boxes, and the waste liquid recovery system is used for recovering the dyeing liquid in the dyeing boxes.
Preferably, the platform is a grating plate, a gap is arranged between the grating plate and the dyeing box bottom plate, a magnetic stirrer is arranged in the gap, and the dyeing liquid supply system and the waste liquid recovery system are communicated with the dyeing box bottom plate through a pipeline.
Preferably, the dyeing box is fixed in the shell through a hollow connecting rod, and a line connected with the magnetic stirrer penetrates through the hollow connecting rod.
Preferably, the lid is installed to dyeing box top, the lid is connected with drive arrangement, drive arrangement is used for the drive the lid removes to open and shut dyeing box.
Preferably, lid downside is provided with downwardly extending's fore-set, the fore-set lower extreme is in during the lid lock the top tightly place in the smear of platform.
Preferably, the plurality of dyeing boxes are arranged in a straight line in sequence above the inside of the shell, and the dyeing solution supply system and the waste liquid recovery system are arranged below the inside of the shell.
Preferably, a cover capable of opening and closing is installed above the housing, and an operation panel is arranged outside the housing.
Preferably, a temperature and humidity detection and adjustment device is arranged in the shell.
Preferably, dyeing liquor supply system includes dyeing fluid reservoir and pressure generator, be provided with intake pipe and drain pipe on the dyeing fluid reservoir, the intake pipe upper end is connected pressure generator, the intake pipe lower extreme is located the inside top of dyeing fluid reservoir, the drain pipe lower extreme is located the inside below of dyeing fluid reservoir, the drain pipe upper end is connected the dyeing box, waste liquid recovery system includes waste liquid holding vessel and negative pressure generator.
Preferably, the dyeing device comprises three dyeing liquid tanks, wherein each dyeing liquid tank is connected with one pressure generator, and the three dyeing liquid tanks are a Rueger dyeing liquid stock tank, a Rueger dyeing liquid diluent tank and a distilled water tank respectively.
The invention provides a smear dyeing machine which comprises a shell, a dyeing liquid supply system, a waste liquid recovery system and a plurality of dyeing boxes, wherein the dyeing liquid supply system, the waste liquid recovery system and the plurality of dyeing boxes are arranged in the shell, a platform used for supporting a smear is arranged in each dyeing box, the plurality of dyeing boxes are communicated with the dyeing liquid supply system and the waste liquid recovery system, the dyeing liquid supply system is used for conveying dyeing liquid to the dyeing boxes, and the waste liquid recovery system is used for recovering the dyeing liquid in the dyeing boxes. The slide glass of dry cell smear is placed on the platform of dyeing box in the working process, and dyeing liquor supply system carries the dyeing liquor to the dyeing box after the dyeing box is closed, then the waste liquid recovery system starts to retrieve the dyeing liquor in the dyeing box, takes out the smear that dyeing was accomplished. Realize mechanical intelligent dyeing through equipment, replace artifical dyeing, promote work efficiency, dyeing concentration and dyeing time accurate control promote the dyeing quality, and then promote the cell identification rate of accuracy.
Drawings
FIG. 1 is a schematic external view of an embodiment of a smear dyeing machine according to the present invention;
FIG. 2 is a schematic diagram of the internal structure of one embodiment of the smear dyeing machine provided by the present invention;
fig. 3 is a schematic structural diagram of a staining box in one embodiment of the smear staining machine provided by the present invention.
Detailed Description
The core of the invention is to provide a smear dyeing machine, which realizes mechanical intelligent dyeing through equipment, improves the working efficiency, has accurate dyeing concentration and dyeing time, improves the dyeing quality and has high cell identification accuracy.
In order that those skilled in the art will better understand the disclosure, the invention will be described in further detail with reference to the accompanying drawings and specific embodiments.
Referring to fig. 1 to 3, fig. 1 is a schematic external structural view of an embodiment of a smear dyeing machine provided in the present invention; FIG. 2 is a schematic diagram of the internal structure of one embodiment of the smear dyeing machine provided by the present invention; fig. 3 is a schematic structural diagram of a staining box in one embodiment of the smear staining machine provided by the present invention.
The specific embodiment of the invention provides a smear dyeing machine, which comprises a shell 1, a dyeing liquid supply system 2, a waste liquid recovery system 3 and a plurality of dyeing boxes 4, wherein the dyeing liquid supply system 2, the waste liquid recovery system 3 and the plurality of dyeing boxes 4 are arranged in the shell 1, a platform for supporting a smear is arranged in each dyeing box 4, the plurality of dyeing boxes 4 are communicated with the dyeing liquid supply system 2 and the waste liquid recovery system 3, the dyeing liquid supply system 2 is used for conveying dyeing liquid to the dyeing boxes 4, and the waste liquid recovery system 3 is used for recovering the dyeing liquid in the dyeing boxes 4.
The slide glass of dry cell smear is placed on the platform of dyeing box 4 in the course of the work, and dyeing liquor supply system 2 carries the dyeing liquor to dyeing box 4 after dyeing box 4 seals, then waste liquid recovery system 3 starts the dyeing liquor of retrieving in the dyeing box 4, takes out the smear that the dyeing was accomplished. Realize mechanical intelligent dyeing through equipment, replace artifical dyeing, promote work efficiency, dyeing concentration and dyeing time accurate control promote the dyeing quality, and then promote the cell identification rate of accuracy.
Specifically, the platform is the grid plate 41 of horizontal setting, can make the dyeing liquid pass through, is provided with the interval between grid plate 41 and the 4 bottom plates of dyeing box, is provided with magnetic stirrers 42 in the interval, and dyeing liquid supply system 2 and waste liquid recovery system 3 pass through the 4 bottom plates of pipeline intercommunication dyeing box. The staining solution enters the staining box 4 from the bottom of the staining box 4 and overflows the smear through the grating plate 41, meanwhile, the magnetic stirrer 42 starts to stir the staining solution, and then the staining solution can also be discharged from the bottom of the staining box 4. The platform may take other configurations, such as a multi-point support structure, or may take other types of agitators. Further, the dyeing box 4 is fixed in the shell 1 through a hollow connecting rod, and a power supply line and a control line of the magnetic stirrer 42 penetrate through the hollow connecting rod, so as to be connected with a control system of the device.
In the smear dyeing machine provided by the embodiment of the invention, in order to facilitate the smear to enter and exit the dyeing box 4 and ensure the sealing effect of the dyeing box 4, an opening is arranged above the dyeing box 4, a box cover 43 is arranged at the opening, a rubber sealing strip is arranged below the box cover 43 in order to ensure the sealing effect, a driving device is arranged in the shell 1, the driving device is connected with the box cover through a connecting rod, the box cover 43 is driven to move up and down, and the dyeing box 4 is opened and closed. The driving device can be driven by a motor or air pressure, and the invention is in the protection scope.
Further, in order to guarantee that the smear is stable in the dyeing process, the lower side surface of the box cover 43 is provided with a downwardly extending jacking column 44, the lower end of the jacking column 44 tightly pushes the smear placed on the platform when the box cover 43 is buckled, the position of the smear is fixed, and particularly, the two jacking columns 44 are arranged and respectively push the two ends of the smear tightly.
In order to facilitate the arrangement of a plurality of smears, a plurality of staining boxes 4 are arranged in sequence in a straight line above the inside of the shell 1, and the staining solution supply system 2 and the waste liquid recovery system 3 are arranged below the inside of the shell 1, so that the space in the equipment is reasonably arranged. Further, a cover 11 which can be opened and closed is installed above the shell 1, the sealing performance of the equipment is improved, an operation panel 12 is further arranged outside the shell 1, the operation panel 12 is located above the shell 1 and used for displaying equipment information, and meanwhile, the starting and operating parameters of the equipment are controlled.
In order to ensure the dyeing quality, the temperature and the humidity of the dyeing environment need to be accurately controlled, a temperature and humidity detection and adjustment device which comprises a temperature sensor, a humidity sensor, a heater, a cooling fan, a humidity control fan and the like can be arranged in the shell 1, the temperature and the humidity in the shell 1 are detected in real time, and the temperature and the humidity are adjusted, so that the stability of the dyeing environment is ensured, and other types of detection and adjustment devices can be adopted and are within the protection range of the invention.
On the basis of the smear dyeing machine that each above-mentioned embodiment provided, dyeing liquor supply system 2 includes dyeing fluid reservoir and pressure generator 21, be provided with intake pipe and drain pipe on the dyeing fluid reservoir, pressure generator 21 is connected to the intake pipe upper end, the intake pipe lower extreme is located the inside top of dyeing fluid reservoir, the drain pipe lower extreme is located the inside below of dyeing fluid reservoir, dyeing box 4 is connected to the drain pipe upper end, push dyeing box 4 through pressure generator 21 with the dyeing liquid in the dyeing fluid reservoir, waste liquid recovery system 3 includes waste liquid holding vessel and negative pressure generator, the start-up of negative pressure generator is retrieved the dyeing liquid in dyeing box 4 to the waste liquid holding vessel after the dyeing is accomplished. Specifically, the dyeing method adopts the Rueger dyeing, and comprises three dyeing liquid tanks, wherein each dyeing liquid tank is connected with a pressure generator 21, and the three dyeing liquid tanks are a Rueger dyeing liquid stock tank 22, a Rueger dyeing liquid diluent tank 23 and a distilled water tank 24 respectively.
The working process is as follows: the slide of dry cell smear is placed on the grid plate 41 in the open staining cassette 4, the drive means moves the cassette cover 43 down, the cassette cover 43 closes the staining cassette 4, and the knock-out post 44 pushes the smear tightly. The pressure generator 21 operates and pressurizes, so that stock solution in the Rueger dyeing solution stock solution tank 22 is conveyed into the dyeing box 4, the liquid amount at least passes 0.2cm of smear in the dyeing box 4, the pressure generator 21 operates again after 1min, air is pressed into the Rueger dyeing solution diluent tank 23, diluent in the Rueger dyeing solution diluent tank 23 is conveyed into the dyeing box 4 through a pipeline, the liquid amount is consistent with the stock solution in the dyeing box 4, a magnetic stirrer 42 is started, and the liquid in the dyeing box 4 is mixed uniformly. After a period of time, the magnetic stirrer 42 is stopped, and the negative pressure generator is started to suck out all the liquid in the dyeing box 4 to the waste liquid storage tank, and then the pressure generator 41 is operated to press air into the distilled water tank 24, and the distilled water in the distilled water tank 24 is transported to the dyeing box 4 through the pipeline, so as to fill the dyeing box 4, and the magnetic stirrer 42 is started. After a period of time the magnetic stirrer 42 is stopped and simultaneously the negative pressure generator is started, all the liquid in the staining box 4 is sucked out to the waste liquid storage tank, the box cover 43 is opened and the smear is taken out to complete the staining. The operating parameters can also be adjusted according to the situation and are all within the protection scope of the invention.
The above details the smear dyeing machine provided by the present invention. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (10)
1. The utility model provides a smear dyeing machine, its characterized in that, including casing (1) and install in inside dyeing liquor supply system (2), waste liquid recovery system (3) and a plurality of dyeing box (4) of casing (1), inside being provided with of dyeing box (4) is used for supporting the platform of smear, and is a plurality of dyeing box (4) all communicate dyeing liquor supply system (2) with waste liquid recovery system (3), dyeing liquor supply system (2) be used for to dyeing box (4) carry the dyeing liquor, waste liquid recovery system (3) are used for retrieving the dyeing liquor in the dyeing box (4).
2. The smear dyeing machine according to claim 1, wherein the platform is in particular a grid plate (41), a space is provided between the grid plate (41) and the dyeing box (4) bottom plate, a magnetic stirrer (42) is provided in the space, the dyeing liquid supply system (2) and the waste liquid recovery system (3) are in communication with the dyeing box (4) bottom plate through a pipeline.
3. Smear dyeing machine according to claim 2 characterized in that the dyeing box (4) is fixed inside the shell (1) by a hollow connecting rod passing inside a line connecting the magnetic stirrer (42).
4. Smear dyeing machine according to claim 1 characterized in that above the dyeing box (4) is mounted a box cover (43), to which box cover (43) is connected a driving device for driving the box cover (43) to move for opening and closing the dyeing box (4).
5. A smear dyeing machine according to claim 4 characterized in that said box cover (43) is provided with a downwardly extending top pillar (44) at its lower side, said top pillar (44) at its lower end abutting the smear placed on said platform when said box cover (43) is snapped.
6. The smear dyeing machine according to claim 1, wherein a plurality of the dyeing boxes (4) are arranged in a straight line in sequence above the inside of the housing (1), the dyeing liquid supply system (2) and the waste liquid recovery system (3) being arranged below the inside of the housing (1).
7. Smear dyeing machine according to claim 6, characterized in that above the housing (1) is mounted a lid (11) that can be opened and closed, the housing (1) being externally provided with an operating panel (12).
8. Smear dyeing machine according to claim 1 characterized in that a temperature and humidity detection regulating device is provided inside the housing (1).
9. The smear dyeing machine according to any one of claims 1 to 8, wherein the staining solution supply system (2) comprises a staining solution tank and a pressure generator (21), the staining solution tank is provided with an air inlet pipe and a liquid outlet pipe, the upper end of the air inlet pipe is connected with the pressure generator (21), the lower end of the air inlet pipe is positioned above the interior of the staining solution tank, the lower end of the liquid outlet pipe is positioned below the interior of the staining solution tank, the upper end of the liquid outlet pipe is connected with the staining box (4), and the waste liquid recovery system (3) comprises a waste liquid storage tank and a negative pressure generator.
10. The smear dyeing machine according to claim 9, comprising three said stain liquid tanks, each connected to one said pressure generator (21), three said stain liquid tanks being a raw Reynolds stain liquid tank (22), a DirRunner stain liquid tank (23) and a distilled water tank (24), respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011400879.0A CN112414826A (en) | 2020-12-04 | 2020-12-04 | Smear dyeing machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011400879.0A CN112414826A (en) | 2020-12-04 | 2020-12-04 | Smear dyeing machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112414826A true CN112414826A (en) | 2021-02-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011400879.0A Pending CN112414826A (en) | 2020-12-04 | 2020-12-04 | Smear dyeing machine |
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| Country | Link |
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| CN (1) | CN112414826A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
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|---|---|---|---|---|
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| CN203732340U (en) * | 2013-12-23 | 2014-07-23 | 湖南省天骑医学新技术有限公司 | Automatic negative pressure staining and dyeing machine |
| CN203908840U (en) * | 2014-06-09 | 2014-10-29 | 马艳华 | Microcomputer-controlled liquid-based cell sheet preparation dyeing system |
| KR20160131786A (en) * | 2015-05-08 | 2016-11-16 | (주)로봇앤드디자인 | Auto cell stainer |
| CN106950094A (en) * | 2017-05-11 | 2017-07-14 | 麦克奥迪（厦门）医疗诊断***有限公司 | Mini multifunctional dyeing work station and colouring method |
| CN107796682A (en) * | 2016-08-31 | 2018-03-13 | 希森美康株式会社 | Film preparation preparation facilities and film preparation preparation method |
| CN208847561U (en) * | 2018-09-30 | 2019-05-10 | 南阳医学高等专科学校第一附属医院 | A kind of bone marrow smear dyeing apparatus |
| CN210638982U (en) * | 2019-08-27 | 2020-05-29 | 段昊 | Slide frame |
-
2020
- 2020-12-04 CN CN202011400879.0A patent/CN112414826A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202141618U (en) * | 2010-10-30 | 2012-02-08 | 湖南省天骑医学新技术有限公司 | Cytological and bacterial staining and preparation machine |
| CN203732340U (en) * | 2013-12-23 | 2014-07-23 | 湖南省天骑医学新技术有限公司 | Automatic negative pressure staining and dyeing machine |
| CN203908840U (en) * | 2014-06-09 | 2014-10-29 | 马艳华 | Microcomputer-controlled liquid-based cell sheet preparation dyeing system |
| KR20160131786A (en) * | 2015-05-08 | 2016-11-16 | (주)로봇앤드디자인 | Auto cell stainer |
| CN107796682A (en) * | 2016-08-31 | 2018-03-13 | 希森美康株式会社 | Film preparation preparation facilities and film preparation preparation method |
| CN106950094A (en) * | 2017-05-11 | 2017-07-14 | 麦克奥迪（厦门）医疗诊断***有限公司 | Mini multifunctional dyeing work station and colouring method |
| CN208847561U (en) * | 2018-09-30 | 2019-05-10 | 南阳医学高等专科学校第一附属医院 | A kind of bone marrow smear dyeing apparatus |
| CN210638982U (en) * | 2019-08-27 | 2020-05-29 | 段昊 | Slide frame |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114279798A (en) * | 2021-04-25 | 2022-04-05 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
| CN114279798B (en) * | 2021-04-25 | 2024-04-02 | 福州安林生物科技有限公司 | Full-automatic immunohistochemical staining instrument and staining method thereof |
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