CN112410180B - Nucleic acid extraction device - Google Patents
Nucleic acid extraction device Download PDFInfo
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- CN112410180B CN112410180B CN202011233591.9A CN202011233591A CN112410180B CN 112410180 B CN112410180 B CN 112410180B CN 202011233591 A CN202011233591 A CN 202011233591A CN 112410180 B CN112410180 B CN 112410180B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a nucleic acid extraction device, which comprises a bottom plate, a vertical plate arranged on the bottom plate and an extraction pipe arranged on the bottom plate, wherein an installation groove is formed in the bottom plate, the extraction pipe is arranged in the installation groove, a first liquid outlet is formed in the bottom of the extraction pipe, a second liquid outlet matched with the first liquid outlet is formed in the bottom of the installation groove, a first connecting pipe is arranged in the extraction pipe, a limiting disc is arranged at the top of the first connecting pipe, a first connecting plate is arranged on the vertical plate, a connecting ring matched with the limiting disc is arranged on the first connecting plate, and the connecting ring is made of an electromagnet; the vertical plate is provided with a mounting plate, the mounting plate is provided with a first driving motor, and the output shaft of the first driving motor is provided with a stirring rod.
Description
Technical Field
The invention belongs to the technical field of nucleic acid extraction, and particularly relates to a nucleic acid extraction device.
Background
In the operation of extracting nucleic acid by a magnetic bead method, whether the magnetic beads are fully mixed with free nucleic acid or not, whether the magnetic beads are completely adsorbed by a magnetic rod or not is important to the nucleic acid extraction effect, especially the magnetic bead mixing operation and the magnetic bead adsorption of a large-volume sample. For the conventional small-capacity nucleic acid extraction work, when nucleic acid is extracted, magnetic beads and free nucleic acid are uniformly mixed by means of up-and-down reciprocating motion of a magnetic rod and rotary motion of a magnetic rod sleeve and liquid suction and discharge modes of a liquid transfer device, the magnetic beads adsorb the nucleic acid, and then the magnetic beads are adsorbed on the outer wall of the magnetic rod sleeve by the magnetic rod, so that one step of the nucleic acid extraction work is completed.
Nucleic acid is usually extracted by a nucleic acid extractor, the nucleic acid extractor generally adsorbs the nucleic acid by using magnetic beads, and finally the nucleic acid is separated from the magnetic beads to finish nucleic acid extraction, so that the collection degree of the magnetic beads is in direct proportion to the extraction degree of the nucleic acid, and the magnetic beads are distributed at all positions of a test tube when a sample is mixed due to smaller magnetic bead particles, and are easy to miss when the magnetic beads are adsorbed by using a magnetic rod; in addition, as various liquids are required to be added during extraction, the volume of the liquid in the test tube is larger, the volume requirement on the test tube is higher, and if the volume of the test tube is increased, the distribution of the magnetic beads is wider, so that the collection difficulty of the magnetic beads is increased.
Disclosure of Invention
The invention provides a nucleic acid extraction mechanism for improving the extraction rate in order to overcome the defects in the prior art.
In order to achieve the above purpose, the present invention adopts the following technical scheme: the utility model provides a nucleic acid extraction device, includes the bottom plate, locates riser on the bottom plate and locate extraction tube on the bottom plate, be equipped with the mounting groove on the bottom plate, the extraction tube is located in the mounting groove, the extraction tube bottom is equipped with first liquid outlet, the mounting groove bottom be equipped with first liquid outlet matched with second liquid outlet, be equipped with first connecting tube in the extraction tube, first connecting tube top is equipped with the spacing dish, be equipped with first connecting plate on the riser, be equipped with on the first connecting plate with spacing dish matched with go-between, the go-between is made for the electro-magnet; the vertical plate is provided with a mounting plate, the mounting plate is provided with a first driving motor, and the output shaft of the first driving motor is provided with a stirring rod; when the nucleic acid extraction operation is performed, a sample is placed in an extraction tube, then a lysate is placed in the extraction tube, a stirring rod is inserted into the extraction tube, a first driving motor drives the stirring rod to rotate, the stirring rod rotates in the extraction tube to stir liquid in the extraction tube, after sample cells are cracked, a connecting ring is electrified to generate magnetic force, the magnetic force is transferred to a first connecting tube, magnetic beads are adsorbed on the first connecting tube, most of the magnetic beads are positioned at the inner wall of the extraction tube due to the stirring effect of the stirring rod, and the magnetic beads can be rapidly adsorbed after the first connecting tube is in magnetic communication; after the magnetic beads are adsorbed on the first connecting pipe, the first liquid outlet is opened, and liquid in the extracting pipe is discharged from the first liquid outlet, so that the liquid volume in the extracting pipe is prevented from being increased due to the fact that multiple liquids are mixed in the extracting pipe; after the liquid in the extraction tube is discharged, the connecting ring is powered off, washing buffer solution is put in from the liquid inlet tube, the magnetic beads and the nucleic acid are washed by using the washing buffer solution, and the stirring rod stretches out into the extraction tube again, so that the magnetic beads are fully mixed with the washing buffer solution; after the magnetic bead drug absorption is completed, the operation is repeated to discharge the liquid in the extraction tube, and the elution buffer solution is added into the extraction tube until the nucleic acid is separated from the magnetic beads, and then the nucleic acid is taken out to complete the nucleic acid extraction; the liquid in the extraction tube is timely discharged after each step of treatment, so that the increase of the volume of the liquid in the extraction tube is avoided, the extraction rate of nucleic acid is influenced, and the magnetic beads are easier to collect; the stirring rod and the first connecting pipe are matched with each other, so that a large number of magnetic beads are positioned at the inner wall of the first connecting pipe, the magnetic beads can be adsorbed quickly, the magnetic beads are prevented from being discharged together with liquid, and the extraction rate of nucleic acid is improved.
The extraction pipe is provided with a liquid inlet pipe, the vertical plate is provided with a first movable groove, a second connecting plate is rotatably connected in the first movable groove, and the second connecting plate is provided with a second connecting pipe matched with the liquid inlet pipe; the vertical plate is provided with a first installation block, an installation cavity is arranged in the first installation block, a partition plate is arranged in the installation cavity, a first liquid storage cavity is separated by the partition plate in the installation cavity, and a liquid storage tank is arranged on the first installation block; the first liquid storage cavity is communicated with the second connecting pipe.
The second connecting plate is provided with a first connecting block which is fixedly connected to the second connecting plate through a screw, the second connecting plate is provided with an infusion main pipe, one end of the infusion main pipe is provided with an infusion branch pipe, and the second connecting pipe is provided with a first liquid inlet matched with the infusion branch pipe; the first connecting block is provided with a second connecting block, the second connecting block is provided with a first sliding groove, and the side wall of the second connecting pipe is provided with a first sliding block matched with the first sliding groove.
The infusion branch pipe one end is equipped with the rubber circle, infusion is responsible for one end and is equipped with the retainer plate, first stock solution intracavity wears to be equipped with first fly leaf, be equipped with first movable chamber on the first fly leaf, be equipped with in the first movable chamber with infusion is responsible for matched with third connecting pipe, be equipped with on the first fly leaf and be used for the intercommunication first stock solution chamber with the infusion chamber of third connecting pipe.
A second sliding block is arranged on the side wall of the third connecting pipe, a second sliding groove matched with the second sliding block is arranged in the first movable cavity, a liquid outlet of the first liquid storage cavity is arranged at one end of the second sliding groove, and a second liquid inlet hole communicated with the third connecting pipe is arranged on the second sliding block; the bottom plate is provided with a driving component for driving the first movable plate to move downwards, and when the first movable plate moves downwards, the third connecting pipe is sleeved on the fixed ring.
The first movable plate is provided with a first groove matched with the fixed ring, the first movable cavity is formed in the inner wall of the first groove, a second movable cavity is formed above the first groove, the bottom of the second movable cavity is provided with a first through cavity communicated with the first groove and a second through cavity communicated with the first movable cavity, the second movable cavity is rotatably connected with a transmission plate, one end of the transmission plate is movably connected with a first push plate, the other end of the transmission plate is movably connected with a second push plate, the first push plate is arranged in the first through cavity in a penetrating mode, the second push plate is arranged in the second through cavity in a penetrating mode, the inner wall of the first movable cavity is movably connected with a third connecting plate, one end of the third connecting plate is movably connected with a fourth connecting plate, and the third connecting plate is movably connected with the fourth connecting plate.
The first movable plate is characterized in that a third sliding block is arranged on the side wall of the first movable plate, a third sliding groove matched with the third sliding block is arranged on the partition plate, a first connecting spring is arranged on the third sliding block, a plurality of second liquid storage cavities are arranged on the liquid storage box, the second liquid storage cavities are arranged at intervals, a second driving motor is arranged in the mounting cavity, the liquid storage box is arranged on an output shaft of the second driving motor, and a third liquid inlet is formed in the top of the first liquid storage cavity.
The novel movable floor comprises a bottom plate, and is characterized in that a third movable cavity is arranged on the bottom plate, a second movable groove is formed in the upper portion of the third movable cavity, the driving assembly comprises a first pushing block penetrating through the second movable groove and a second movable plate arranged in the third movable cavity, a fourth movable cavity is arranged above the third movable cavity, a third movable plate is arranged in the fourth movable cavity, a second connecting spring is arranged at the top of the third movable plate, a plurality of pushing rods are arranged at the bottom of the third movable plate, a supporting spring is arranged at the bottom of the first pushing block, the second movable groove is communicated with the fourth movable cavity, a connecting rope is arranged on the second movable plate, and the other end of the connecting rope is fixedly connected to the first movable plate.
The bottom plate is provided with a plurality of third connecting blocks, the third connecting blocks are arranged at the top of the second movable grooves, the third connecting blocks are provided with third movable grooves, limiting springs are arranged in the third movable grooves, one ends of the limiting springs are provided with limiting blocks, and the first pushing blocks are provided with limiting grooves matched with the limiting blocks; the limiting block is provided with a second push block, and the second push block penetrates through the third movable groove.
The extraction tube bottom is equipped with the fifth movable chamber, be equipped with in the fifth movable chamber with first liquid outlet matched with dog, be equipped with third connecting spring on the dog, the mounting groove bottom is equipped with the fourth movable groove, be equipped with the movable block in the fourth movable groove, be equipped with the lug on the movable block, the dog bottom be equipped with lug matched with second recess, be equipped with the head rod on the movable block, be equipped with the second connecting rod on the head rod, be equipped with the third ejector pad on the second connecting rod, fourth movable groove one side is equipped with the sixth movable chamber, sixth movable intracavity is equipped with first cylinder.
The invention has the following advantages: the liquid in the extraction tube is timely discharged after each step of treatment, so that the increase of the volume of the liquid in the extraction tube is avoided, the extraction rate of nucleic acid is influenced, and the magnetic beads are easier to collect; the stirring rod and the first connecting pipe are matched with each other, so that a large number of magnetic beads are positioned at the inner wall of the first connecting pipe, the magnetic beads can be adsorbed quickly, the magnetic beads are prevented from being discharged together with liquid, and the extraction rate of nucleic acid is improved.
Drawings
Fig. 1 is a schematic structural view of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 is a cross-sectional view taken along A-A in fig. 2.
Fig. 4 is an enlarged view at a in fig. 3.
Fig. 5 is an enlarged view at B in fig. 3.
Fig. 6 is a cross-sectional view taken along B-B in fig. 2.
Fig. 7 is an enlarged view at C in fig. 6.
Fig. 8 is a cross-sectional view taken along S-S in fig. 2.
Fig. 9 is an enlarged view at M in fig. 8.
Fig. 10 is a cross-sectional view taken along line C-C of fig. 2.
Fig. 11 is an enlarged view of D in fig. 10.
Fig. 12 is a cross-sectional view taken along D-D in fig. 2.
Fig. 13 is an enlarged view at E in fig. 12.
Fig. 14 is a right side view of the present invention.
Fig. 15 is a cross-sectional view taken along F-F in fig. 14.
Fig. 16 is an enlarged view at F in fig. 15.
Fig. 17 is a cross-sectional view taken along G-G in fig. 14.
Fig. 18 is an enlarged view at G in fig. 17.
Fig. 19 is a cross-sectional view taken along H-H in fig. 14.
Fig. 20 is an enlarged view at H in fig. 19.
Fig. 21 is a cross-sectional view taken along line I-I in fig. 14.
Fig. 22 is an enlarged view of fig. 21 at I.
Fig. 23 is a cross-sectional view taken along J-J in fig. 14.
Fig. 24 is an enlarged view at J in fig. 23.
Fig. 25 is an enlarged view at K in fig. 24.
Fig. 26 is a cross-sectional view taken along line K-K in fig. 14.
Fig. 27 is an enlarged view at L in fig. 26.
Detailed Description
As shown in fig. 1-27, a nucleic acid extraction device comprises a bottom plate 1, a vertical plate 2 arranged on the bottom plate 1 and an extraction tube 3 arranged on the bottom plate 1, wherein an installation groove 11 is arranged on the bottom plate 1, the extraction tube 3 is arranged in the installation groove 11, a first liquid outlet 31 is arranged at the bottom of the extraction tube 3, a second liquid outlet 15 matched with the first liquid outlet 31 is arranged at the bottom of the installation groove 11, a first connecting tube 4 is arranged in the extraction tube 3, a limiting disc 41 is arranged at the top of the first connecting tube 4, a first connecting plate 231 is arranged on the vertical plate 2, a connecting ring 23 matched with the limiting disc 41 is arranged on the first connecting plate 231, and the connecting ring 23 is made of an electromagnet; the vertical plate 2 is provided with a mounting plate 21, the mounting plate 21 is provided with a first driving motor 251, and the output shaft of the first driving motor 251 is provided with a stirring rod 26; when the nucleic acid extraction operation is performed, a sample is placed in an extraction tube, then a lysate is placed in the extraction tube, a stirring rod is inserted into the extraction tube, a first driving motor drives the stirring rod to rotate, the stirring rod rotates in the extraction tube to stir liquid in the extraction tube, after sample cells are cracked, a connecting ring is electrified to generate magnetic force, the magnetic force is transferred to a first connecting tube, magnetic beads are adsorbed on the first connecting tube, most of the magnetic beads are positioned at the inner wall of the extraction tube due to the stirring effect of the stirring rod, and the magnetic beads can be rapidly adsorbed after the first connecting tube is in magnetic communication; after the magnetic beads are adsorbed on the first connecting pipe, the first liquid outlet is opened, and liquid in the extracting pipe is discharged from the first liquid outlet, so that the liquid volume in the extracting pipe is prevented from being increased due to the fact that multiple liquids are mixed in the extracting pipe; after the liquid in the extraction tube is discharged, the connecting ring is powered off, washing buffer solution is put in from the liquid inlet tube, the magnetic beads and the nucleic acid are washed by using the washing buffer solution, and the stirring rod stretches out into the extraction tube again, so that the magnetic beads are fully mixed with the washing buffer solution; after the magnetic bead drug absorption is completed, the operation is repeated to discharge the liquid in the extraction tube, and the elution buffer solution is added into the extraction tube until the nucleic acid is separated from the magnetic beads, and then the nucleic acid is taken out to complete the nucleic acid extraction; the liquid in the extraction tube is timely discharged after each step of treatment, so that the increase of the volume of the liquid in the extraction tube is avoided, the extraction rate of nucleic acid is influenced, and the magnetic beads are easier to collect; the stirring rod and the first connecting pipe are matched with each other, so that a large number of magnetic beads are positioned at the inner wall of the first connecting pipe, the magnetic beads can be adsorbed quickly, the magnetic beads are prevented from being discharged together with liquid, and the extraction rate of nucleic acid is improved.
The bottom of the mounting plate is provided with a second air cylinder 22, a piston rod of the second air cylinder is provided with a fifth connecting plate 24, the bottom of the fifth connecting plate is provided with a second mounting block 25, the bottom of the second mounting block is provided with an equipment cavity, a first driving motor is arranged in the equipment cavity, an output shaft of the first driving motor is provided with a third mounting block 252, the bottom of the third mounting block is of an arc-shaped structure, the third mounting block is provided with a connecting groove, a stirring rod is arranged in the connecting groove, the connecting groove is obliquely arranged, the stirring rod is obliquely arranged along with the connecting groove, the inner wall of the connecting groove is provided with a rubber film, the connecting effect of the stirring rod and the connecting groove is increased under the action of the rubber film, and the stirring rod is prevented from falling out of the connecting groove; utilize the slope setting of puddler, increase the contact effect of puddler and liquid to increase the stirring effect of puddler to liquid, make the liquid in the extraction tube produce more violent motion under the puddler effect, promote the mixing effect of liquid and magnetic bead.
The top of the mounting groove is provided with a fourth connecting pipe 12, the fourth connecting pipe is provided with a heating block, and the depth of the mounting groove is increased by the fourth connecting pipe, so that the fourth connecting pipe can be stably fixed in the mounting groove, and the use reliability of the extraction pipe is ensured; when the stirring rod is inserted into the extraction pipe, the heating block works to heat the liquid in the extraction pipe, and the heating block and the stirring rod are matched with each other to improve the stirring effect on the liquid.
The extracting pipe 3 is provided with a liquid inlet pipe 33, the vertical plate 2 is provided with a first movable groove 210, a second connecting plate 220 is rotatably connected in the first movable groove 210, and the second connecting plate 220 is provided with a second connecting pipe 240 matched with the liquid inlet pipe 33; the vertical plate 2 is provided with a first mounting block 27, a mounting cavity is arranged in the first mounting block, a partition 274 is arranged in the mounting cavity, the partition 274 partitions a first liquid storage cavity 272 in the mounting cavity, and the first mounting block 27 is provided with a liquid storage tank 28; the first liquid storage cavity 272 is communicated with the second connecting pipe 240; when the extraction pipe is not used or the extraction pipe is disassembled, the second connecting plate is turned upwards, and the second connecting plate is turned to be in a vertical state so as to directly disassemble the extraction pipe; after the extraction pipe is installed, the second connecting plate is turned downwards, the second connecting plate is turned to be in a horizontal state, the second connecting pipe rotates to the top of the feeding pipe, the second connecting pipe is pushed downwards, the second connecting pipe is sleeved on the feeding pipe, liquid in the liquid storage tank enters the second connecting pipe through the feeding pipe, the liquid in the second connecting pipe enters the liquid inlet pipe, and the liquid is added into the extraction pipe so as to process a sample in the extraction pipe; under the setting of the liquid storage tank, liquid can be automatically added into the extraction tube, so that the sample treatment is automatically carried out, and the nucleic acid extraction difficulty is reduced.
The first movable groove top is equipped with fixed spring 2101, and the fixed spring bottom is equipped with the fixed plate 2102, and the second connecting plate rotates to on the vertical state after, and the second connecting plate supports on the fixed plate, utilizes the cooperation of fixed plate and fixed spring to play fixed action to the second connecting plate, prevents that the second connecting plate from automatic turning out from first movable groove.
The top of the extraction tube is provided with a positioning block 35, the bottom of the limiting disc is provided with a positioning groove matched with the positioning block, the first connecting tube is provided with a first liquid inlet matched with the liquid inlet, and the liquid inlet is aligned with the first liquid inlet under the matching of the positioning block and the positioning groove, so that liquid is added into the extraction tube from the liquid inlet, and the extraction of nucleic acid is completed.
The second connecting plate 220 is provided with a first connecting block 230, the first connecting block 230 is fixedly connected to the second connecting plate 220 through a screw 2201, the second connecting plate 220 is provided with an infusion main pipe 250, one end of the infusion main pipe 250 is provided with an infusion branch pipe 2502, the second connecting pipe 240 is provided with a first liquid inlet 2402 matched with the infusion branch pipe 2502, the first connecting block 230 is provided with a second connecting block 2301, the second connecting block 2301 is provided with a first sliding groove 2302, and the side wall of the second connecting pipe 240 is provided with a first sliding block 241 matched with the first sliding groove 2302; the first connecting block is provided with a first through hole matched with the second connecting pipe, the second connecting plate is provided with a second through hole corresponding to the first through hole, and the transfusion branch pipe penetrates through the inner wall of the second through hole; the port of the main infusion tube is made of hard material, and the main infusion tube body is made of soft material, namely, the main infusion tube body can deform, so that the main infusion tube is prevented from obstructing the rotation of the second connecting plate; after the extraction tube is installed, the second connecting plate is turned downwards, the second connecting plate is turned to be in a horizontal state, the second connecting plate is located at the top of the liquid inlet tube, the second connecting tube is pushed downwards, the liquid inlet tube is inserted into the second connecting tube, the first liquid inlet hole on the second connecting tube is moved to one side of the liquid conveying branch tube, the liquid conveying branch tube is aligned with the first liquid inlet hole, liquid in the liquid conveying branch tube enters the second connecting tube, so that the liquid is automatically added into the extraction tube, and the nucleic acid extraction operation on a sample is completed.
A rubber ring 2503 is arranged at one end of the transfusion branch pipe 2502, the side wall of the second connecting pipe is propped against the rubber ring, the rubber ring is deformed under the action of the second connecting pipe, the connection effect of the transfusion branch pipe and the first liquid inlet hole is improved, and liquid is prevented from leaking from the connection part of the second connecting pipe and the transfusion branch pipe; a fixed ring 2501 is arranged at one end of the main infusion tube 250, a first movable plate 260 is arranged in the first liquid storage cavity 272 in a penetrating manner, a first movable cavity 2609 is arranged on the first movable plate 260, a third connecting tube 2607 matched with the main infusion tube 250 is arranged in the first movable cavity 2609, and an infusion cavity 2741 used for communicating the first liquid storage cavity 272 with the third connecting tube 2607 is arranged on the first movable plate 260; after the extraction tube is installed, the fixing ring enters the first groove, the third connecting tube moves towards the outer side of the first movable cavity, the third connecting tube is sleeved on the fixing ring, and the liquid storage tank is communicated with the infusion main tube, so that the solution in the liquid storage tank is fed into the extraction tube.
A second sliding block 26071 is arranged on the side wall of the third connecting pipe 2607, a second sliding groove matched with the second sliding block 26071 is arranged in the first movable cavity 2609, a liquid outlet of the first transfusion cavity 272 is arranged at one end of the second sliding groove, and a second liquid inlet 26072 communicated with the third connecting pipe 2607 is arranged on the second sliding block 26071; the bottom plate 1 is provided with a driving component for driving the first movable plate 260 to move downwards, and when the first movable plate 260 moves downwards, the third connecting pipe 2607 is sleeved on the fixed ring 2501; when the first movable plate is not stressed, the first movable plate is positioned above the fixed ring, and the third connecting pipe is positioned in the first movable cavity; after the extraction pipe is installed, the second connecting plate is turned downwards, the second connecting plate is rotated to a horizontal position and then pushes the second connecting pipe downwards, the second connecting pipe is sleeved on the liquid inlet pipe, the driving assembly drives the first movable plate to move downwards, the first groove moves to the position of the fixed ring, and the third connecting pipe moves to one side of the fixed ring; when the third connecting pipe is aligned with the fixing ring, the third connecting pipe moves outwards, so that the third connecting pipe is sleeved on the fixing ring, the connection between the third connecting pipe and the infusion main pipe is completed, and the liquid storage tank in the extraction pipe is communicated, so that required liquid is automatically added into the extraction pipe; when the third connecting pipe moves to the outer side of the first movable cavity, the second sliding block moves in the second sliding groove, the second sliding block moves to one end of the second sliding groove, a second liquid inlet hole on the second sliding block is aligned with a liquid outlet of the first liquid storage cavity, liquid in the first liquid storage cavity flows into the third connecting pipe through the first liquid storage cavity, the liquid is added into the extraction pipe by the third connecting pipe, and samples in the extraction pipe are processed.
The first movable plate 260 is provided with a first groove 26010 matched with the fixed ring 2501, the first movable cavity 2609 is arranged on the inner wall of the first groove 26010, a second movable cavity 2601 is arranged above the first groove 26010, the bottom of the second movable cavity 2601 is provided with a first through cavity communicated with the first groove 26010 and a second through cavity communicated with the first movable cavity 2609, a transmission plate 2602 is rotatably connected in the second movable cavity 2601, one end of the transmission plate 2602 is movably connected with a first push plate 2603, the other end of the transmission plate 2602 is movably connected with a second push plate 2604, the first push plate 2603 is arranged in the first through cavity in a penetrating mode, and the second push plate 2604 is arranged in the second through cavity in a penetrating mode; a fourth sliding block is arranged on the side wall of the first push plate, a fourth sliding groove matched with the fourth sliding block is arranged on the inner wall of the first through cavity, and a reset spring is arranged on the fourth sliding block; a third connecting plate 2605 is movably connected to the inner wall of the first movable cavity 2609, one end of the third connecting plate 2605 is movably connected to a fourth connecting plate 2606, and the third connecting pipe 2607 is movably connected to the fourth connecting plate 2606; when the first movable plate moves downwards, the first groove moves downwards relative to the fixed ring, the fixed ring enters the first groove, the first push plate is pushed to move upwards under the action of the fixed ring, one end of the first push plate pushes the transmission plate to move upwards, the transmission plate rotates around the connecting point to enable the other end to push the second push plate to move downwards, the second push plate abuts against the connecting point of the third connecting plate and the fourth connecting plate, the third connecting plate and the fourth connecting plate are pushed to rotate under the action of the second push plate, the third connecting plate and the fourth connecting plate rotate in the straight line direction, the third connecting plate and the fourth connecting plate push the third connecting pipe to extend out of the first movable cavity, the third connecting pipe is pressed on the side wall of the vertical plate, the third connecting pipe is sleeved on the fixed ring, and the liquid storage tank and the extraction pipe are conducted, so that liquid is automatically added into the extraction pipe.
A third sliding block 2608 is arranged on the side wall of the first movable plate 260, a third sliding groove matched with the third sliding block 2608 is arranged on the partition 274, a first connecting spring 26081 is arranged on the third sliding block 2608, and the first connecting spring provides supporting force for the third sliding block, so that the first movable plate is positioned above the fixed ring when not stressed, and the first liquid storage cavity is in a closed state; the liquid storage tank 28 is provided with a plurality of second liquid storage cavities 281, the plurality of second liquid storage cavities 281 are arranged at intervals, a second driving motor 271 is arranged in the mounting cavity, the liquid storage tank 28 is arranged on an output shaft of the second driving motor 271, the top of the first liquid storage cavity 272 is provided with a third liquid inlet 273, and the bottom of the second liquid storage cavity is provided with a liquid outlet 282; the number of the preferred second liquid storage cavities is three, the top of the second liquid storage cavity is provided with a second liquid inlet, and the three second liquid storage cavities are respectively provided with a pyrolysis liquid, a washing buffer liquid and an elution buffer liquid; after the third connecting pipe is matched with the fixed ring, placing a sample into the extraction pipe, driving the liquid storage tank to rotate by the second driving motor, enabling the second liquid storage cavity filled with the pyrolysis liquid to rotate to the position above the third liquid inlet hole by the second driving motor, enabling the liquid outlet hole to be aligned with the third liquid inlet hole, and enabling the pyrolysis liquid to enter the extraction pipe through the third connecting pipe, so that cells in the extraction pipe are subjected to a pyrolysis step; after enough pyrolysis liquid is added into the extraction tube, the second driving motor drives the liquid storage tank to rotate, so that the side wall of the liquid storage tank rotates the top of the third liquid inlet hole, and the pyrolysis liquid does not enter the extraction tube any more; after the cracking step in the extraction tube is finished, discharging the waste liquid in the extraction tube, and driving the liquid storage tank to rotate by the second driving motor again, so that the second liquid storage cavity filled with the washing buffer solution rotates to the position of the third liquid inlet hole, and adding the washing buffer solution into the extraction tube; after the magnetic bead washing is finished, the elution buffer solution is added into the extraction tube by repeating the above operation, and the second liquid storage cavity filled with the lysate rotates to one side of the third liquid inlet hole, so that the samples of the next group are processed, and the samples are processed more conveniently.
The bottom plate 1 is provided with a third movable cavity 190, a second movable groove 120 is arranged above the third movable cavity 190, the driving assembly comprises a first push block 18 penetrating through the second movable groove 120 and a second movable plate 130 arranged in the third movable cavity 190, a fourth movable cavity 140 is arranged above the third movable cavity 190, a third movable plate 150 is arranged in the fourth movable cavity 140, a second connecting spring 1501 is arranged at the top of the third movable plate 150, a plurality of push rods 1502 are arranged at the bottom of the third movable plate 150, a supporting spring 181 is arranged at the bottom of the first push block 18, the second movable groove 120 is communicated with the fourth movable cavity 140, a connecting rope 1301 is arranged on the second movable plate 130, and the other end of the connecting rope 1301 is fixedly connected to the first movable plate 260; after the extraction pipe is installed, the pushing block is pushed downwards to move towards the bottom of the second movable groove, the air flow in the second movable groove is pushed to move towards the fourth movable cavity under the action of the pushing block, the air flow enters the fourth movable cavity to increase the air pressure at the top of the third movable plate, the third movable plate is pushed to move downwards under the action of the air pressure, the push rod moves downwards along with the third movable plate, the push rod pushes the second movable to move downwards after entering the third movable cavity, the second movable plate pulls the connecting rope when moving towards the bottom of the third movable cavity, the connecting rope pulls the first movable plate to move downwards to provide downward movement power for the first movable plate, the first movable plate moves downwards under the driving component, and therefore the third connecting pipe and the fixed ring form connection fit, and the connection of the liquid storage tank and the extraction pipe is completed.
The bottom plate 1 is provided with a plurality of third connecting blocks 19, the third connecting blocks 19 are arranged at the top of the second movable grooves 120, the third connecting blocks 19 are provided with third movable grooves 191, limiting springs 192 are arranged in the third movable grooves 191, one ends of the limiting springs 192 are provided with limiting blocks 193, and the first pushing blocks 18 are provided with limiting grooves 182 matched with the limiting blocks 193; a second push block 194 is arranged on the limiting block 193, and the second push block 194 is arranged in the third movable groove 191 in a penetrating manner; when pushing down the ejector pad, the ejector pad moves to second movable groove bottom, and the spacing groove removes to stopper one side, promotes stopper in the spacing inslot motion under spacing spring effect, and the stopper is embedded into the spacing inslot and is fixed the ejector pad to fix the ejector pad on the assigned position, guarantee the fixed effect of second fly leaf to first fly leaf, make liquid reserve tank and extraction tube form stable cooperation.
The bottom of the extraction tube 3 is provided with a fifth movable cavity 34, a stop block 32 matched with the first liquid outlet 31 is arranged in the fifth movable cavity 34, a third connecting spring 321 is arranged on the stop block 32, a fourth movable groove 17 is arranged at the bottom of the mounting groove 11, a movable block 16 is arranged in the fourth movable groove 17, a bump 164 is arranged on the movable block 16, a second groove matched with the bump 164 is arranged at the bottom of the stop block 32, a first connecting rod 161 is arranged on the movable block 16, a second connecting rod 162 is arranged on the first connecting rod 161, a third pushing block 163 is arranged on the second connecting rod 162, a sixth movable cavity 160 is arranged on one side of the fourth movable groove 17, and a first cylinder 1601 is arranged in the sixth movable cavity 160; after the extraction pipe is arranged in the mounting groove, the convex block is inserted into the first groove, and the movable block and the stop block form connection fit; after the processing of the magnetic beads in the extraction tube is completed, the first air cylinder pushes the third pushing block to move, the third pushing block drives the movable block to move, the stop block is made to move to one end of the Du Wei movable cavity, the liquid in the extraction tube is discharged after the first liquid outlet is opened, the liquid is prevented from accumulating in the extraction tube and increasing the liquid volume, and therefore the magnetic beads are fully collected.
The bottom plate bottom is equipped with supporting leg 170, and supporting leg one side is equipped with first material receiving box 13, and first material receiving box bottom is equipped with the second material receiving box, and first material receiving box length is greater than second material receiving box length, and the waste liquid is collected in first material receiving box, and after magnetic bead and nucleic acid separation, the second material receiving box removes to second leakage fluid dram bottom, utilizes the second material receiving box to collect nucleic acid, separates nucleic acid and waste liquid mutually, reduces the nucleic acid and collects the degree of difficulty.
The driving motor and the air cylinder in the drawings of the application are schematic diagrams, and the specific structure of the driving motor and the air cylinder is the same as that of the motor and the air cylinder in the prior art.
Claims (4)
1. The utility model provides a nucleic acid extraction device, includes bottom plate (1), locates riser (2) on bottom plate (1) and locate extraction tube (3) on bottom plate (1), be equipped with mounting groove (11) on bottom plate (1), extraction tube (3) are located in mounting groove (11), its characterized in that: the device is characterized in that a first liquid outlet (31) is formed in the bottom of the extraction pipe (3), a second liquid outlet (15) matched with the first liquid outlet (31) is formed in the bottom of the mounting groove (11), a first connecting pipe (4) is arranged in the extraction pipe (3), a limiting disc (41) is arranged at the top of the first connecting pipe (4), a first connecting plate (231) is arranged on the vertical plate (2), a connecting ring (23) matched with the limiting disc (41) is arranged on the first connecting plate (231), and the connecting ring (23) is made of an electromagnet; the vertical plate (2) is provided with a mounting plate (21), the mounting plate (21) is provided with a first driving motor (251), and the output shaft of the first driving motor (251) is provided with a stirring rod (26);
the extraction pipe (3) is provided with a liquid inlet pipe (33), the vertical plate (2) is provided with a first movable groove (210), a second connecting plate (220) is rotatably connected in the first movable groove (210), and the second connecting plate (220) is provided with a second connecting pipe (240) matched with the liquid inlet pipe (33); a first mounting block (27) is arranged on the vertical plate (2), a mounting cavity is arranged in the first mounting block, a partition plate (274) is arranged in the mounting cavity, a first liquid storage cavity (272) is partitioned by the partition plate (274) in the mounting cavity, and a liquid storage tank (28) is arranged on the first mounting block (27); the first liquid storage cavity (272) is communicated with the second connecting pipe (240);
the infusion device comprises a first connecting plate (220), a second connecting plate (220) and a second connecting block (2301), wherein the first connecting block (230) is fixedly connected to the second connecting plate (220) through a screw (2201), an infusion main pipe (250) is arranged on the second connecting plate (220), an infusion branch pipe (2502) is arranged at one end of the infusion main pipe (250), a first liquid inlet (2402) matched with the infusion branch pipe (2502) is arranged on the second connecting pipe (240), a second connecting block (2301) is arranged on the first connecting block (230), a first sliding groove (2302) is arranged on the second connecting block (2301), and a first sliding block (241) matched with the first sliding groove (2302) is arranged on the side wall of the second connecting pipe (240);
a rubber ring (2503) is arranged at one end of the infusion branch pipe (2502), a fixed ring (2501) is arranged at one end of the infusion main pipe (250), a first movable plate (260) is arranged in the first liquid storage cavity (272) in a penetrating mode, a first movable cavity (2609) is arranged on the first movable plate (260), a third connecting pipe (2607) matched with the infusion main pipe (250) is arranged in the first movable cavity (2609), and an infusion cavity (2741) used for communicating the first liquid storage cavity (272) with the third connecting pipe (2607) is arranged on the first movable plate (260);
a second sliding block (26071) is arranged on the side wall of the third connecting pipe (2607), a second sliding groove matched with the second sliding block (26071) is arranged in the first movable cavity (2609), a liquid outlet of the first liquid storage cavity (272) is arranged at one end of the second sliding groove, and a second liquid inlet (26072) communicated with the third connecting pipe (2607) is arranged on the second sliding block (26071); the bottom plate (1) is provided with a driving component for driving the first movable plate (260) to move downwards, and when the first movable plate (260) moves downwards, the third connecting pipe (2607) is sleeved on the fixed ring (2501);
the first movable plate (260) is provided with a first groove (26010) matched with the retainer ring (2501), the first movable cavity (2609) is arranged on the inner wall of the first groove (26010), a second movable cavity (2601) is arranged above the first groove (26010), the bottom of the second movable cavity (2601) is provided with a first through cavity communicated with the first groove (26010) and a second through cavity communicated with the first movable cavity (2609), a transmission plate (2602) is rotatably connected in the second movable cavity (2601), one end of the transmission plate (2602) is movably connected with a first push plate (2603), the other end of the transmission plate (2602) is movably connected with a second push plate (2604), the first push plate (2603) is arranged in the first through cavity in a penetrating mode, the second push plate (2604) is arranged in the second through cavity in a penetrating mode, the inner wall of the first movable cavity (2609) is movably connected with a third connecting plate (2605), and the third connecting plate (2606) is movably connected with the fourth connecting plate (2606);
be equipped with third slider (2608) on first fly leaf (260) lateral wall, be equipped with on baffle (274) with third slider (2608) matched with third spout, be equipped with first coupling spring (26081) on third slider (2608), be equipped with a plurality of second liquid storage cavities (281) on liquid reserve tank (28), a plurality of second liquid storage cavities (281) interval sets up, be equipped with second driving motor (271) in the installation cavity, liquid reserve tank (28) are located on second driving motor (271) output shaft, first liquid storage cavity (272) top is equipped with third feed liquor hole (273).
2. The nucleic acid extraction apparatus according to claim 1, wherein: be equipped with third movable chamber (190) on bottom plate (1), third movable chamber (190) top is equipped with second movable groove (120), drive assembly is including wearing to locate first ejector pad (18) in second movable groove (120) and locate second movable plate (130) in third movable chamber (190), third movable chamber (190) top is equipped with fourth movable chamber (140), be equipped with third movable plate (150) in fourth movable chamber (140), third movable plate (150) top is equipped with second coupling spring (1501), third movable plate (150) bottom is equipped with a plurality of push rods (1502), first ejector pad (18) bottom is equipped with supporting spring (181), second movable groove (120) with fourth movable chamber (140) are communicated with each other, be equipped with connecting rope (1301) on second movable plate (130), connecting rope (1301) other end link firmly on first movable plate (260).
3. The nucleic acid extraction apparatus according to claim 2, wherein: the bottom plate (1) is provided with a plurality of third connecting blocks (19), the third connecting blocks (19) are arranged at the tops of the second movable grooves (120), the third connecting blocks (19) are provided with third movable grooves (191), limiting springs (192) are arranged in the third movable grooves (191), one ends of the limiting springs (192) are provided with limiting blocks (193), and the first pushing blocks (18) are provided with limiting grooves (182) matched with the limiting blocks (193); the limiting block (193) is provided with a second pushing block (194), and the second pushing block (194) penetrates through the third movable groove (191).
4. The nucleic acid extraction apparatus according to claim 1, wherein: the extraction tube (3) bottom is equipped with fifth movable chamber (34), be equipped with in fifth movable chamber (34) with first liquid outlet (31) matched with dog (32), be equipped with third connecting spring (321) on dog (32), mounting groove (11) bottom is equipped with fourth movable groove (17), be equipped with movable block (16) in fourth movable groove (17), be equipped with lug (164) on movable block (16), dog (32) bottom be equipped with lug (164) matched with second recess, be equipped with head rod (161) on movable block (16), be equipped with second connecting rod (162) on the head rod (161), be equipped with third ejector pad (163) on the second connecting rod (162), fourth movable groove (17) one side is equipped with sixth movable chamber (160), be equipped with first cylinder (1601) in sixth movable chamber (160).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011233591.9A CN112410180B (en) | 2020-11-06 | 2020-11-06 | Nucleic acid extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011233591.9A CN112410180B (en) | 2020-11-06 | 2020-11-06 | Nucleic acid extraction device |
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| CN112410180A CN112410180A (en) | 2021-02-26 |
| CN112410180B true CN112410180B (en) | 2023-07-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202011233591.9A Active CN112410180B (en) | 2020-11-06 | 2020-11-06 | Nucleic acid extraction device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005323516A (en) * | 2004-05-13 | 2005-11-24 | Hitachi Maxell Ltd | Device for extracting nucleic acid |
| CN110272808A (en) * | 2018-03-13 | 2019-09-24 | 武汉医蒂生物科技有限公司 | A kind of nucleic acid extraction system |
| CN110564607A (en) * | 2019-09-11 | 2019-12-13 | 长春技特生物技术有限公司 | Full-automatic nucleic acid extraction amplification micro-fluidic chip dynamic quantitative detection integrated device |
| CN111100860A (en) * | 2018-10-26 | 2020-05-05 | 南京金斯瑞生物科技有限公司 | Large-scale magnetic purification system |
| CN111304290A (en) * | 2020-03-16 | 2020-06-19 | 通用生物***（安徽）有限公司 | Nucleic acid extraction method for gene sequencing |
-
2020
- 2020-11-06 CN CN202011233591.9A patent/CN112410180B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005323516A (en) * | 2004-05-13 | 2005-11-24 | Hitachi Maxell Ltd | Device for extracting nucleic acid |
| CN110272808A (en) * | 2018-03-13 | 2019-09-24 | 武汉医蒂生物科技有限公司 | A kind of nucleic acid extraction system |
| CN111100860A (en) * | 2018-10-26 | 2020-05-05 | 南京金斯瑞生物科技有限公司 | Large-scale magnetic purification system |
| CN110564607A (en) * | 2019-09-11 | 2019-12-13 | 长春技特生物技术有限公司 | Full-automatic nucleic acid extraction amplification micro-fluidic chip dynamic quantitative detection integrated device |
| CN111304290A (en) * | 2020-03-16 | 2020-06-19 | 通用生物***（安徽）有限公司 | Nucleic acid extraction method for gene sequencing |
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