CN112391303B - Bifidobacterium powder and preparation process of probiotics thereof - Google Patents
Bifidobacterium powder and preparation process of probiotics thereof Download PDFInfo
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- CN112391303B CN112391303B CN201910751796.7A CN201910751796A CN112391303B CN 112391303 B CN112391303 B CN 112391303B CN 201910751796 A CN201910751796 A CN 201910751796A CN 112391303 B CN112391303 B CN 112391303B
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- bifidobacterium
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- 239000000843 powder Substances 0.000 title claims abstract description 96
- 241000186000 Bifidobacterium Species 0.000 title claims abstract description 83
- 239000006041 probiotic Substances 0.000 title claims abstract description 24
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000001694 spray drying Methods 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 28
- 229920001202 Inulin Polymers 0.000 claims abstract description 20
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims abstract description 20
- 229940029339 inulin Drugs 0.000 claims abstract description 20
- 239000011159 matrix material Substances 0.000 claims abstract description 20
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 19
- 229930195725 Mannitol Natural products 0.000 claims abstract description 19
- 239000000594 mannitol Substances 0.000 claims abstract description 19
- 235000010355 mannitol Nutrition 0.000 claims abstract description 19
- 235000013336 milk Nutrition 0.000 claims abstract description 19
- 239000008267 milk Substances 0.000 claims abstract description 19
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- 235000012239 silicon dioxide Nutrition 0.000 claims abstract description 19
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 19
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 16
- 239000002994 raw material Substances 0.000 claims abstract description 15
- 229920001983 poloxamer Polymers 0.000 claims abstract description 14
- 229960000502 poloxamer Drugs 0.000 claims abstract description 14
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims abstract description 13
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims abstract description 13
- 241001052560 Thallis Species 0.000 claims abstract description 7
- 239000000047 product Substances 0.000 claims description 29
- 230000000529 probiotic effect Effects 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 238000004806 packaging method and process Methods 0.000 claims description 15
- 235000020183 skimmed milk Nutrition 0.000 claims description 13
- 238000002156 mixing Methods 0.000 claims description 12
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- 230000008569 process Effects 0.000 claims description 10
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- 239000011812 mixed powder Substances 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 6
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 5
- 235000019700 dicalcium phosphate Nutrition 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 5
- 238000007873 sieving Methods 0.000 claims description 4
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 3
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 238000007689 inspection Methods 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 229920001993 poloxamer 188 Polymers 0.000 claims description 3
- 229940044519 poloxamer 188 Drugs 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000011049 filling Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 24
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 7
- 239000011575 calcium Substances 0.000 abstract description 7
- 229910052791 calcium Inorganic materials 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 239000003223 protective agent Substances 0.000 description 3
- 208000005577 Gastroenteritis Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 235000013365 dairy product Nutrition 0.000 description 2
- 235000003599 food sweetener Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
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- 235000012055 fruits and vegetables Nutrition 0.000 description 2
- 238000011194 good manufacturing practice Methods 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000003595 mist Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012946 outsourcing Methods 0.000 description 2
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- 239000000243 solution Substances 0.000 description 2
- 239000003765 sweetening agent Substances 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- 238000009777 vacuum freeze-drying Methods 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
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- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical class OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 240000001046 Lactobacillus acidophilus Species 0.000 description 1
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 description 1
- 201000010538 Lactose Intolerance Diseases 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
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- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 235000013361 beverage Nutrition 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
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- 210000001072 colon Anatomy 0.000 description 1
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- 238000010924 continuous production Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940095079 dicalcium phosphate anhydrous Drugs 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
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- 239000000945 filler Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229940039695 lactobacillus acidophilus Drugs 0.000 description 1
- 229960002160 maltose Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 229940116406 poloxamer 184 Drugs 0.000 description 1
- 229920001992 poloxamer 407 Polymers 0.000 description 1
- 229940044476 poloxamer 407 Drugs 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 239000007909 solid dosage form Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1234—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G1/00—Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/30—Cocoa products, e.g. chocolate; Substitutes therefor
- A23G1/32—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds
- A23G1/42—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
- A23G1/423—Cocoa products, e.g. chocolate; Substitutes therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
- A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
- A23G3/34—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof
- A23G3/36—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds
- A23G3/364—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
- A23G3/366—Sweetmeats, confectionery or marzipan; Processes for the preparation thereof characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins containing microorganisms, enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Genetics & Genomics (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicinal Preparation (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a bifidobacteria powder and a preparation process of probiotics thereof, wherein a protective matrix containing maltose, calcium hydrophosphate and poloxamer is selected, and thalli are mixed with the matrix before spray drying, so that the heat resistance and stress resistance activity of bifidobacteria is improved, and the survival rate of the bifidobacteria in spray drying is obviously improved; not only can play a role in improving the survival rate of the bifidobacteria, but also can ensure that the dried bifidobacteria powder has good fluidity, thereby facilitating the subsequent preparation processing. The bifidobacterium powder prepared by the method is used as a raw material, a proper amount of milk powder, inulin, mannitol and silicon dioxide are added as auxiliary materials, and the powder packaged in a single dose is prepared by applying modern industrial technology.
Description
Technical Field
The invention relates to a preparation process of bifidobacterium powder and a preparation process of probiotics containing the bifidobacterium powder.
Background
Probiotics refers to a micro-ecological preparation which can maintain the balance of flora in human body and has beneficial effect on human health. The oral administration of sufficient active probiotics is helpful for relieving acute and chronic gastroenteritis, treating diarrhea, improving digestion, relieving symptoms such as lactose intolerance and the like. Bifidobacteria are a common probiotic, and the thallus preparation of the bifidobacteria is divided into a liquid preparation and a solid preparation. The former is prepared through traditional fermentation culture to obtain bifidobacterium liquid as the preparation product, and the latter is prepared through proliferation of probiotics, freeze drying, spray drying, embedding and other steps. The liquid preparation is easy to be polluted or the strain is degenerated along with the prolonging of the preservation time, the activation culture and the expansion culture are needed before the use, the solid microbial inoculum can be directly put into the culture, the expansion culture is not needed before the fermentation, and the preservation cost is lower. At present, the bifidobacterium solid preparation process is usually vacuum freeze drying, spray drying, fluidized bed drying, microwave vacuum drying and the like. Vacuum freeze drying has little damage to thallus subcells and high viable bacteria rate, but is easily influenced by factors such as pre-freezing temperature, freezing speed, vacuum degree and the like, and has long time consumption and high cost; the fluidized bed has longer drying time, and when the material stays for uneven time, the drying is uneven; in the microwave vacuum drying, polar molecules (such as thallus moisture) synchronously rotate at high speed along with microwave frequency, so that friction heat is instantaneously generated on materials, the surfaces and the interiors of the materials are simultaneously heated, a large number of thallus water molecules escape, and drying is realized. The spray drying is to directly spray the prepared bacterial suspension into mist through an atomizing device, and the mist is directly contacted with high-temperature air in a drying chamber to carry out heat and mass transfer, the drying time is extremely short, the material temperature is low, the product dispersibility and dissolubility are good, the process is simple, the equipment cost is relatively low, and the spray drying is particularly suitable for industrial continuous production and is widely used in the fields of medicines and foods.
However, the solution is fully mixed with high-temperature air in the spray drying process, so that the temperature of the bacteria liquid is sharply increased, and a large amount of bifidobacteria are killed. Therefore, a series of works have been made in the prior art on how to improve the survival rate of bifidobacteria. CN108070542A firstly preparing a bifidobacterium biomembrane, and then carrying out spray drying on the bifidobacterium biomembrane to ensure that the survival rate of the bifidobacterium spray-dried can reach more than 55 percent; CN105341940A, CN1306021C and CN102210659B disclose methods for improving the survival rate of bifidobacteria by optimizing the formulation of dry protective agent through screening of sugar, protein, salt, etc., or improving the survival rate through microencapsulation.
In addition, the bifidobacterium powder obtained in the spray drying process is usually processed into solid dosage forms such as tablets and the like for convenient oral administration. It is therefore also desirable for the powder obtained after spray drying to have good flowability (no caking ) and good formability (the tablets produced have a relatively high breaking force). In the prior art, an anti-caking agent and an adhesive are additionally added in the forming process, so that the subsequent preparation process is complicated.
In order to overcome the defects of the prior art, the process for developing the novel bifidobacterium powder has wide application prospect. Meanwhile, the bifidobacterium powder prepared by the method is used as a raw material, a proper amount of milk powder, inulin, mannitol and silicon dioxide are added as auxiliary materials, and the powder packaged in single dose is prepared by applying modern industrial technology.
Disclosure of Invention
The invention aims to provide a spray drying process of bifidobacterium powder, which has the advantages of high survival rate of bifidobacterium and good fluidity and formability of dried powder and solves the problems of low survival rate of the bifidobacterium after spray drying and complex subsequent preparation process in the prior art.
To achieve the above object, a first aspect of the present invention provides a method for producing a bifidobacterium powder, comprising:
a preparation method of bifidobacterium powder is characterized by comprising the following steps:
a. culturing Bifidobacterium in culture medium, and collecting thallus;
b. uniformly mixing the thalli and a spray drying matrix to obtain a thalli mixed solution;
c. spray drying, and collecting Bacillus bifidus powder;
wherein in step b the spray dried matrix comprises maltose, dibasic calcium phosphate and poloxamer.
In one embodiment, the medium of step a is TPY medium; the bifidobacterium strain is Chr, Hansen BB-12; the method comprises the following specific steps: inoculating Bifidobacterium into TPY culture medium at 5%, culturing at 37 + -3 deg.C for 16-24 hr under anaerobic condition, and culturing at 4 deg.C for 5000--1Centrifuging for 10-20min, discarding supernatant, cleaning thallus with sterilized distilled water, and centrifuging under the same condition to obtain Bifidobacterium thallus.
In one embodiment, the spray dried matrix of step b comprises: 7-13% by weight of skim milk, preferably 10% by weight of skim milk; 5-10% by weight of maltose, preferably 7-8% by weight; 1-2% by weight of calcium hydrogen phosphate, preferably 1.4-1.6% by weight; 0.3-0.5% by weight of poloxamer, preferably 0.35-0.45% by weight; the balance being water.
In a certain embodiment the poloxamer is selected from poloxamer 184, poloxamer 188 or poloxamer 407.
In one embodiment, the spray drying conditions of step c are: and c, drying the thallus mixed solution obtained in the step b by a spray dryer at the speed of 4-6ml/min, wherein the inlet temperature is 130-150 ℃, and the outlet temperature is 50-70 ℃. Spray drying to obtain Bifidobacterium powder.
The second aspect of the invention provides bifidobacterium powder prepared by the method.
The third aspect of the invention provides the application of the bacterium powder in the fields of food, health products and biology, and the application comprises the preparation of solid beverages, dairy products, fermented fruits and vegetables, chocolate, candies, microecologics, probiotic tablets, probiotic capsules and probiotic powder.
In one embodiment of the invention, the use comprises preparing a probiotic powder comprising bifidobacteria prepared according to the present invention, said probiotic powder further comprising lactobacillus acidophilus; preferably the probiotic powder further comprises a prebiotic component, for example comprising at least one of fructooligosaccharides, galactooligosaccharides, xylooligosaccharides, breast milk oligosaccharides.
In one embodiment of the invention, the bifidobacterium powder is used for producing dairy products, bean products or fruit and vegetable products by fermentation.
In one embodiment of the invention, bifidobacterium powder prepared by the method is used as a raw material, a proper amount of milk powder, inulin, mannitol and silicon dioxide are added as auxiliary materials, and the probiotic powder packaged in a single dose is prepared by modern industrial technology.
The probiotic powder of the invention adopts milk powder, inulin, mannitol and silicon dioxide as auxiliary materials. Wherein, the milk powder: as a diluent of the product, the product also has the function of improving the mouthfeel; inulin: is a multiplication factor of bifidobacterium, and can greatly increase beneficial bacteria in colon and reduce pathogenic bacteria and putrefying bacteria by taking inulin every day; mannitol: the sweetener has the characteristics of no hygroscopicity, good chemical stability and the like, is used as a filling agent of the product, and also plays a role of a sweetener in the product due to certain sweetness; silicon dioxide: the product can be used as anticaking agent for improving powder flowability and preventing agglomeration.
The production process of the probiotic powder is simple, easy to operate and easy to control the product quality, and the main process route is as follows: weighing, mixing, subpackaging, packaging, obtaining finished products, inspecting and warehousing. Because the product has no terminal sterilization, except that the sanitary indexes of the raw and auxiliary materials and the packaged product are strictly controlled, the sanitary control (including production environment, equipment, containers and personnel) in the whole production process is strictly executed according to the GMP (good manufacturing practice) regulation of the health-care food, so as to ensure that the sanitary indexes of the product meet the quality requirements.
1) Preparation of raw materials: the bifidobacterium powder, the milk powder, the inulin, the mannitol and the silicon dioxide which are prepared by the method are strictly placed in a clean raw material workshop for standby after being subjected to outsourcing in a buffer room according to the procedure of entering a clean area of 10 ten thousand levels.
2) Pretreatment of raw materials and auxiliary materials: respectively sieving Bifidobacterium powder, milk powder, inulin, mannitol and silicon dioxide with 60 mesh sieve.
3) Weighing: according to the total formula amount and the formula proportion of each batch of ingredients, the bifidobacterium powder, the milk powder, the inulin, the mannitol and the silicon dioxide are accurately weighed, and the check is performed by a person skilled in the art.
4) Mixing: firstly, the bifidobacterium powder and the mannitol are gradually and uniformly mixed according to the equal quantity to obtain mixed powder B, and the mixed powder B, the milk powder, the inulin and the silicon dioxide are added into a three-dimensional mixer to be mixed for 25-35 minutes.
5) Subpackaging: subpackaging with a particle packaging machine according to requirements.
6) And (3) outer packaging: and (3) putting the subpackaged samples into a small box of 10 bags/box in an outer packaging room, labeling, boxing and packaging.
7) And (4) checking: and (5) inspecting the finished product according to the delivery inspection requirement of the product.
8) Warehousing: and (5) warehousing and storing the finished product after the finished product is qualified.
The equivalent incremental operation method comprises the steps of firstly taking a small amount of components, adding an equivalent large amount of components, then taking a large amount of components which are equivalent to the mixture, uniformly mixing, increasing by the times until all the large amount of components are added, uniformly mixing, and sieving.
Advantageous effects
The invention selects a specific protective matrix, and the thalli and the matrix are mixed before spray drying, so that the heat resistance and stress resistance activity of the bifidobacterium is improved, the survival rate of the bifidobacterium spray drying is obviously improved, and the survival rate of the bifidobacterium spray drying can reach more than 60 percent. Solves the problem of low survival rate of the bifidobacterium in the spray drying process.
The matrix before spray drying not only can play a role in improving the survival rate of the bifidobacteria, but also can ensure that the dried bifidobacteria powder has good fluidity, thereby facilitating the subsequent preparation processing.
The bifidobacterium powder prepared by the method is used as a raw material, a proper amount of milk powder, inulin, mannitol and silicon dioxide are added as auxiliary materials, and the powder packaged in a single dose is prepared by applying modern industrial technology.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Bifidobacterium species: hansen BB-12.
Inoculating Bacillus bifidus BB-12 in TPY culture medium at 5%, culturing at 37 + -1 deg.C for 24 hr under anaerobic condition, and culturing at 4 deg.C for 6000r min-1Centrifuging for 15min, discarding supernatant, washing thallus with sterilized distilled water, and centrifuging again under the same conditions to obtain Bifidobacterium thallus.
The obtained cells and the dry protectant were mixed uniformly at a weight/volume ratio (1:100 g/mL). The formula of the spray drying matrix comprises: 10g of skimmed milk powder, 7g of maltose, 1.5g of calcium hydrophosphate, 0.4g of poloxamer and 81.1g of water.
And (3) carrying out spray drying on the mixed solution obtained in the previous step, wherein the spray drying conditions are as follows: the mixed liquid of the thalli is dried by a spray dryer at the speed of 5ml/min, the inlet temperature is 140 ℃, and the outlet temperature is 55 ℃. Spray drying to obtain Bifidobacterium powder. Survival rate of bifidobacterium: 60.1 percent.
The survival rate of bifidobacteria after spray drying was calculated according to the following formula: survival rate of bifidobacterium (viable count after drying) (cfu g)-1Dry matter)/viable count before drying (cfu g)-1Dry matter) × 100%.
Example 2
The spray-dried matrix formula is: 10g of skimmed milk powder, 7g of trehalose, 1.5g of calcium hydrophosphate, 0.4g of poloxamer and 81.1g of water. Other operating conditions were the same as in example 1. Survival rate of bifidobacterium: 52.8 percent.
Example 3
The spray-dried matrix formula is: 10g of skim milk powder, 7g of sucrose, 1.5g of calcium hydrophosphate, 0.4g of poloxamer and 81.1g of water. Other operating conditions were the same as in example 1. Survival rate of bifidobacterium: and (5) 55.3%.
Example 4
The spray-dried matrix formula is: 10g of skimmed milk powder, 7g of maltose, 1.5g of magnesium chloride, 0.4g of poloxamer and 81.1g of water. Other operating conditions were the same as in example 1. Survival rate of bifidobacterium: 48.7 percent.
Example 5
The spray-dried matrix formula is: 10g of skimmed milk powder, 7g of maltose, 1.5g of magnesium chloride, 0.4g of poloxamer and 81.1g of water. Other operating conditions were the same as in example 1. Survival rate of bifidobacterium: 51.4 percent.
Example 6
The spray-dried matrix formula is: 10g of skimmed milk powder, 7g of maltose, 1.5g of sodium dihydrogen phosphate, 0.4g of poloxamer and 81.1g of water. Other operating conditions were the same as in example 1. Survival rate of bifidobacterium: 57.6 percent.
Example 7
The spray-dried matrix formula is: 10g of skim milk powder, 5g of maltose, 1.5g of calcium hydrophosphate, 0.5g of poloxamer 188 and 83g of water. Other operating conditions were the same as in example 1. The angle of repose of the spray-dried bifidobacterium powder is measured by the following method: the funnel is fixed at a certain height above the coordinate paper (the paper is placed on a horizontal table), materials are added from the funnel until the top of the formed stacking cone is just contacted with the bottom of the funnel, the diameter of the cone is measured, and the ratio of the height of the bottom of the funnel to the radius of the cone is used as a tangent value to calculate the angle of repose. The angle of repose of the powder obtained in example 7 was 36.53 degrees.
Example 8
The spray-dried matrix formula is: 10g of skim milk powder, 5g of maltose, 1.5g of calcium hydrophosphate, 0.5g of Tween 80 and 83g of water. Other operating conditions were the same as in example 7. The angle of repose of the powder obtained in example 8 was 37.88 degrees.
Example 9
The spray-dried matrix formula is: 10g of skim milk powder, 5g of maltose, 1.5g of calcium hydrophosphate, 0.5g of SDS and 83g of water. Other operating conditions were the same as in example 7. The angle of repose of the powder obtained in example 8 was 38.47 degrees.
From examples 1 to 6, it can be seen that the dual protective agents of maltose and calcium hydrogen phosphate are selected, so that the heat resistance and stress resistance activity of the bifidobacteria is improved, the spray drying survival rate of the bifidobacteria is remarkably improved, and the spray drying survival rate can reach more than 60%. Solves the problem of low survival rate of the bifidobacterium in the spray drying process.
From examples 7 to 9, it can be seen that the protective agent of the present invention, when added with poloxamer as a surfactant, has the effect of improving the survival rate of bifidobacteria, and can also provide the dried bifidobacteria powder with good fluidity, thereby facilitating the subsequent preparation processing.
EXAMPLE 10 Probiotics powder production Process
1. Preparation of raw materials: the bifidobacterium powder, the milk powder, the inulin, the mannitol and the silicon dioxide which are prepared by the method are strictly placed in a clean raw material workshop for standby after being subjected to outsourcing in a buffer room according to the procedure of entering a clean area of 10 ten thousand levels.
2. Pretreatment of raw materials and auxiliary materials: respectively sieving Bifidobacterium powder, milk powder, inulin, mannitol and silicon dioxide with 60 mesh sieve.
3. Weighing: according to the total formula amount and the formula proportion of each batch of ingredients, the bifidobacterium powder, the milk powder, the inulin, the mannitol and the silicon dioxide are accurately weighed, and the check is performed by a person skilled in the art.
4. Mixing: firstly, the bifidobacterium powder and the mannitol are gradually and uniformly mixed according to the equal quantity to obtain mixed powder B, and the mixed powder B, the milk powder, the inulin and the silicon dioxide are added into a three-dimensional mixer to be mixed for 30 minutes.
5. Subpackaging: subpackaging with a particle packaging machine according to the requirement, wherein each bag is 1.5 g.
6. And (3) outer packaging: and (3) putting the subpackaged samples into a small box of 10 bags/box in an outer packaging room, labeling, boxing and packaging.
7. And (4) checking: and (5) inspecting the finished product according to the delivery inspection requirement of the product.
8. Warehousing: and (5) warehousing and storing the finished product after the finished product is qualified.
The production data of different batches of probiotic powder are as follows:
the names, models and manufacturers of the main production equipment are as follows:
name of production facility | Model number | Manufacturer of the product |
Multi-directional motion mixer | HD100 type | Jiangnan pharmaceutical machinery Co Ltd of Zhejiang Ruian |
Automatic powder packaging machine | F40Ⅱ | HONDON PACKAGING & FOOD MACHINERY Co.,Ltd. |
The main raw material of the probiotic powder is the bifidobacterium powder prepared by the invention, and the powder is prepared by adding a proper amount of auxiliary materials, mixing and directly subpackaging. Before subpackaging, the flowability of the prepared probiotic powder is considered by taking the repose angle as an investigation index, and the measurement result of the repose angle of the mixed powder is as follows:
as can be seen from the results of the angle of repose data in the table above: the average value of the repose angle alpha of the mixed powder measured for six times is 35.47 degrees, and the requirement of directly subpackaging the powder can be met when the alpha is generally considered to be less than or equal to 40 degrees. Therefore, the probiotic powder of the invention is feasible by directly subpackaging the mixed raw materials and auxiliary materials, and the obtained product has good fluidity.
Claims (5)
1. A preparation method of bifidobacterium powder is characterized by comprising the following steps:
a. culturing Bifidobacterium in culture medium, and collecting thallus;
b. uniformly mixing the thalli and a spray drying matrix to obtain a thalli mixed solution;
c. spray drying, and collecting Bacillus bifidus powder;
wherein the spray-dried matrix of step b comprises: 7-13% by weight of skim milk; 5-10% by weight of maltose; 1-2% by weight of calcium hydrogen phosphate; 0.3-0.5% by weight of poloxamer; the balance of water;
the poloxamer is selected from poloxamer 188;
the spray drying conditions of the step c are as follows: b, drying the thallus mixed solution obtained in the step b by a spray dryer at the speed of 4-6ml/min, wherein the inlet temperature is 130-;
wherein the culture medium in the step a is TPY culture medium; the bifidobacterium strain is Chr.Hansen BB-12;
the specific operation of the step a comprises the following steps: inoculating Bifidobacterium into TPY culture medium at 5%, culturing at 37 + -3 deg.C for 16-24 hr under anaerobic condition, and culturing at 4 deg.C for 5000--1Centrifuging for 10-20min, discarding supernatant, cleaning thallus with sterilized distilled water, and centrifuging under the same condition to obtain Bifidobacterium thallus.
2. The method for preparing bifidobacterium powder as claimed in claim 1, wherein the spray-dried matrix of step b comprises: 10% by weight of skim milk; 7-8% parts by weight of maltose; 1.4-1.6% by weight of calcium hydrogen phosphate; 0.35 to 0.45 percent of poloxamer; the balance being water.
3. A powder of bifidobacterium bacteria obtainable by a process according to any one of claims 1 to 2.
4. A method for preparing probiotic powder, comprising the steps of:
1) preparation of raw materials: placing bifidobacterium powder, milk powder, inulin, mannitol and silicon dioxide prepared by the method according to claim 1 into a clean area of 10 ten thousand grade according to a procedure, and immediately placing the bifidobacterium powder, the milk powder, the inulin, the mannitol and the silicon dioxide into a clean raw material workshop for standby after a buffer room is taken out of a package;
2) pretreatment of raw materials and auxiliary materials: respectively sieving bifidobacterium powder, milk powder, inulin, mannitol and silicon dioxide with a 60-mesh sieve for later use;
3) weighing: accurately weighing bifidobacterium powder, milk powder, inulin, mannitol and silicon dioxide according to the total formula amount and the formula proportion of each batch of ingredients;
4) mixing: firstly, sequentially and uniformly mixing bifidobacterium powder and mannitol in equal quantity to obtain mixed powder B, adding the mixed powder B, milk powder, inulin and silicon dioxide into a three-dimensional mixer, and mixing for 25-35 minutes;
5) subpackaging: subpackaging with a particle packaging machine according to requirements;
6) and (3) outer packaging: filling the subpackaged samples into small boxes of 10 bags/box in an outer packaging room, labeling, boxing and packaging;
7) and (4) checking: inspecting the finished product according to the delivery inspection requirement of the product;
8) warehousing: and (5) warehousing and storing the finished product after the finished product is qualified.
5. The method for preparing probiotic powder according to claim 4, characterized in that the mixing time of step 4) is 30 min.
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Denomination of invention: Preparation process of a bifidobacterium powder and its probiotics Effective date of registration: 20231227 Granted publication date: 20220415 Pledgee: Industrial and Commercial Bank of China Limited Chaozhou Branch Pledgor: GUANGDONG CHANGXING BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980074123 |