CN112388868A - Disposal method of disposable culture dish for experiment - Google Patents
Disposal method of disposable culture dish for experiment Download PDFInfo
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- CN112388868A CN112388868A CN202011180078.8A CN202011180078A CN112388868A CN 112388868 A CN112388868 A CN 112388868A CN 202011180078 A CN202011180078 A CN 202011180078A CN 112388868 A CN112388868 A CN 112388868A
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- culture dish
- culture
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- container
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29B—PREPARATION OR PRETREATMENT OF THE MATERIAL TO BE SHAPED; MAKING GRANULES OR PREFORMS; RECOVERY OF PLASTICS OR OTHER CONSTITUENTS OF WASTE MATERIAL CONTAINING PLASTICS
- B29B17/00—Recovery of plastics or other constituents of waste material containing plastics
- B29B17/02—Separating plastics from other materials
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/52—Mechanical processing of waste for the recovery of materials, e.g. crushing, shredding, separation or disassembly
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/62—Plastics recycling; Rubber recycling
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- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Mechanical Engineering (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a disposal method of a disposable culture dish for experiments. According to the difference of the culture in the sex dish: if the culture medium and the culture in the to-be-treated sexual culture dish are suitable to be separated from the culture dish before high-temperature sterilization, and the culture dish does not need high-temperature sterilization, taking out the culture medium and the culture, classifying kitchen garbage after high-temperature sterilization, and completely cleaning the sexual culture dish and then recycling; otherwise, the following method is adopted: s1, vertically and parallelly stacking culture dishes on a perforated plate of the container; s2, filling water into the container and submerging the container over a culture dish; s3, sterilizing the container; s4, pouring out liquid in the container, then flushing the deformed culture dish with a large amount of water while the culture dish is hot, and simultaneously injecting water to submerge the culture dish; s5, repeating S43-5 times. The invention can thoroughly separate the sex culture dish from other impurities to obtain pure plastic materials of the sex culture dish, thereby facilitating subsequent recycling, realizing the recycling of the plastic materials of the sex culture dish, and leading the culture medium and the culture to enter a reasonable disposal way.
Description
Technical Field
The invention relates to a disposal method of a disposable culture dish for experiments, belonging to the technical field of waste treatment.
Background
The disposable culture dish is low in cost, convenient to use, popular with workers in the scientific research and production line, and widely applied to the technical fields of medical treatment, medicines and biology. However, used culture dishes generally contain a culture medium and a residual living culture, such as bacteria, fungi, etc., which are required to be sterilized at high temperature for further disposal according to biosafety.
The disposable culture dish is mostly made of plastics such as polystyrene and the like as main materials, the thermal stability is poor, the culture dish is often deformed after high-temperature sterilization, the culture medium therein is melted and flows out when meeting high temperature, and the culture medium can be solidified again after cooling and is coagulated together with the plastic materials of the culture dish.
Because the main material of the disposable culture dish is plastic, the culture medium and the culture in the disposable culture dish usually have the main components of organic matters and contain a large amount of water. The properties of the two compositions are different, so that the waste disposal methods to which the compositions are applied after being discarded are different. If the waste is directly discarded, the waste is not suitable for subsequent waste treatment.
The garbage landfill is a conventional garbage disposal mode, if a waste disposable culture dish enters a garbage landfill system, organic matters such as a culture medium and the like can be gradually and naturally degraded, but the degradation of plastics often needs decades or even hundreds of years. Because the plastic is difficult to naturally degrade, the permeability of the soil can be influenced by the plastic in the soil, the growth of plant roots and the absorption of nutrients are hindered, and the broken plastic can also become micro plastic. It is reported that 90% of the current edible salts contain micro-plastics. Belgium scientists estimate that a person with seafood hobbies may have 1.1 million plastic granules to eat seafood a year. 500- & gt 1300 million tons of plastic wastes enter the sea every year, and the total weight of the plastic wastes in the sea is expected to exceed the total weight of the fishes by 2050. Therefore, natural degradation of waste plastics in nature is not necessarily an environmentally friendly approach. Waste incineration is another common waste treatment mode, plastic materials are easy to burn, and although the mode can meet the reduction requirement and simultaneously recover part of energy, culture media, cultures and the like contain a large amount of organic substances and moisture, and the burning process consumes energy. In addition, a large amount of light hydrocarbons, nitrides, sulfides, highly toxic dioxin and the like are easily released in the combustion process of organic substances, so that the health of human beings and the ecological environment is directly threatened, and the acid rain is aggravated by HCl gas generated in the incineration process.
Effective treatment of garbage becomes a key link of environmental protection, different types of garbage are classified and treated from the source, reasonable utilization of resources is realized, and the method is a fundamental way for solving the problems. The plastic material with simple components is easy to realize recovery and reuse, and the simple plastic regeneration technology needs to directly form and process the recovered waste plastic after sorting, cleaning, crushing, melting and granulating to produce regenerated products. The plastic is cleaned, selected and classified, which is the basis for the next recycling. The key point of recycling the disposable culture dish is to separate the plastic material of the disposable culture dish from other impurities to obtain a pure clean plastic material of the disposable culture dish.
Disclosure of Invention
The invention provides a disposal method of a waste disposable culture dish for experiments, and particularly relates to a method for separating a pure plastic material of the disposable culture dish from the waste culture dish for laboratory culture and realizing separation of the plastic material from a culture medium and a culture in the plastic material and respectively entering different garbage disposal approaches.
According to the difference of the culture in the disposable culture dish, the following different disposal methods are divided:
if the culture medium and culture in the disposable culture dish to be treated are suitably separated from the disposable culture dish before being autoclaved and the disposable culture dish does not need to be autoclaved, the treatment is performed according to the method 1), otherwise the treatment is performed according to the method 2):
1) opening the cover of the disposable culture dish, and taking out the culture medium and the culture in the disposable culture dish; then cleaning the disposable culture dish to obtain a cleaned disposable culture dish plastic material for recycling;
sterilizing the culture medium and the culture at high temperature, and then putting the culture medium and the culture into kitchen garbage;
the sterilization temperature is 100-121 ℃, and the time is 10-30 min;
2) that is, in the case where it is not appropriate to separate the culture medium, culture and culture dish in advance before the harmless high-temperature sterilization treatment is performed, such as culture for culturing filamentous fungi and the like, for the treatment of such disposable culture dishes, the present invention provides an apparatus and a method for recovering a plastic material in the disposable culture dish as follows:
a device for separating disposable culture dish from culture medium and culture substance therein comprises a container (the container can be a glass beaker or other barrel-shaped container made of high temperature resistant material such as metal) and a grid plate or a multi-hole plate;
the grid plate or the porous plate can be placed in the container, and a space is arranged between the grid plate or the porous plate and the bottom of the container;
the containers with proper sizes can be selected according to the number of disposable culture dishes to be treated, such as 1-5 liters; and matching with containers of different sizes to prepare mesh plates or porous plates with suitable sizes
The material of the grid plate or the porous plate can be metal or other high-temperature-resistant materials;
the aperture of the grid plate or the perforated plate can be 4-10 mm;
the grid plate or the porous plate can be arranged in the container through a triangular bracket or other modes, for example, three triangular brackets are used as supports, and a certain gap is formed between the three triangular brackets and the bottom of the container.
The steps of recovering the plastic material in the disposable culture dish by using the device are as follows:
s1, vertically stacking one or more disposable culture dishes to be processed on the grid plate or the multi-well plate in the device; multiple layers can be stacked;
s2, injecting water into the container to ensure that the water level is as much as possible over the height of the disposable culture dish so as to ensure that the culture dish can float in the water after sterilization;
s3, placing the container in an autoclave for sterilization; under the sterilization condition, the container and the grid plate or the multi-well plate are not damaged, but the disposable culture dish therein is deformed and floats in the liquid in the container; taking out the container when the temperature of the contents in the container is reduced to 70-100 ℃ after the sterilization is finished;
s4, pouring out the liquid in the container, rapidly washing the deformed disposable culture dish with a large amount of water while the container is hot, and simultaneously injecting water into the container to enable the water level to be over the height of the disposable culture dish;
s5, repeating the step S43-5 times, wherein the disposable culture dishes are rapidly cooled in the process, the deformed disposable culture dishes during sterilization and the covers and the bottoms of the single disposable culture dishes are mutually separated, and meanwhile, the culture medium contained in the disposable culture dishes is further dissolved in water; and thoroughly cleaning the separated disposable culture dish again, picking out other solid impurities such as fungus thalli and the like mixed in the disposable culture dish, obtaining a clean plastic material of the disposable culture dish, and collecting the plastic material after drying in the air, so that the plastic material is convenient for further recovery, processing and utilization.
In the above method, in step S1, the cover of the disposable culture dish does not need to be opened.
In the above method, in step S3, the sterilization temperature may be 100 to 121 ℃, and the sterilization time may be 10 to 30 min.
In the above method, the water is tap water.
According to the invention, the culture dishes needing to be subjected to harmless sterilization treatment are vertically placed in the drawer frame and stacked in the container instead of being horizontally stacked in the container layer by layer, so that mutual extrusion and stacking among the culture dishes during high-temperature sterilization can be avoided, and separation among the culture dishes and between the cover and the bottom of the culture dish is facilitated.
When heating sterilization is carried out, water in the container is enabled to submerge the disposable culture dish as much as possible, and the culture dish deformed during heating sterilization can float in the water, so that further deformation and mutual extrusion due to the action of gravity are reduced, and separation between the culture dishes and between the cover and the bottom of the culture dish is facilitated.
When the container is taken out from the autoclave, the temperature of the content of the container is between 70 and 100 ℃, so that the violent temperature drop of the disposable culture dishes in the container in the subsequent operation can be ensured, the deformation is obvious, and the separation effect between different culture dishes and between the cover and the bottom of each culture dish is ensured.
After pouring the liquid in the container, wash the disposable culture dish of deformation with a large amount of running water while hot, make it cool off fast, utilize the expend with heat and contract with cold that violent temperature variation brought, the different positions of the disposable culture dish that warp when high temperature sterilization further deformation still remain the same principle, separate between the different culture dishes and between the lid and the end of each culture dish, avoid interweaving into the caking each other, the clearance of impurity wherein is convenient for is got rid of and is remained the washing of culture medium. Finally obtaining the clean plastic material of the culture dish for subsequent recycling.
When the heating sterilization, disposable culture dish can take place to warp, and culture medium wherein can melt, flow, and the disposable culture dish that will deal with is put on the drawer frame, and when the culture medium flowed, can flow below the drawer frame along the hole on the drawer frame, more is favorable to culture medium and disposable culture dish plastic material's separation.
By the method, the disposable culture dish can be thoroughly separated from other impurities to obtain pure plastic materials of the disposable culture dish, so that the subsequent recycling is facilitated, the recycling of the plastic materials of the disposable culture dish is realized, and the culture medium and the culture substance in the disposable culture dish enter a reasonable disposal way.
Drawings
FIG. 1 is a flow chart of the disposal method of the waste disposable culture dish for experiments provided by the invention.
FIG. 2 is a schematic diagram of the structure of the device for separating the disposable culture dish from the culture medium and the culture substance therein according to the present invention.
The respective symbols in the figure are as follows:
1 container (glass beaker for example), 2 metal mesh plate, 3 metal perforated plate, 4 triangular support.
FIG. 3 is a photograph of disposable petri dishes stacked vertically in a container and submerged as much as possible in water.
FIG. 4 is a photograph of a clean disposable plastic culture dish isolated after sterilization in the method of the present invention.
FIG. 5 is a photograph of the plastic material of the disposable petri dish deformed and coagulated together after direct high temperature sterilization in the laboratory.
Detailed Description
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The treatment is performed according to the following two situations, and the flow chart is shown in fig. 1:
firstly, the culture medium and the culture are separated from the disposable culture dish before harmless high-temperature sterilization, and the culture dish does not need to be subjected to separate special high-temperature sterilization treatment. The method comprises the following steps:
the cover of the disposable culture dish is opened firstly, the culture medium and the culture are taken out, then the disposable culture dish is thoroughly cleaned, and the clean plastic material of the disposable culture dish is obtained for recycling.
Collecting the culture medium and culture, sterilizing at 121 deg.C for 30min, and collecting the sterilized culture medium and culture in kitchen garbage.
Secondly, it is not suitable to separate the culture medium, the culture and the petri dish in advance before the harmless high-temperature sterilization treatment. For example, for culturing filamentous fungi and the like. For the handling of this type of culture dish, the invention provides an apparatus and a method for recycling plastic material from disposable culture dishes as follows.
The device for separating the disposable culture dish from the culture medium and the culture in the disposable culture dish comprises a glass beaker 1, a metal grid plate 2 or a metal porous plate 3, wherein the metal grid plate 2 or the metal porous plate 3 can be placed in the glass beaker 1 and is supported by 3 triangular supports 4. Wherein, a glass beaker 1 with a proper size, such as 1-5 liters, can be selected according to the number of disposable culture dishes to be treated; and a corresponding metal mesh plate 2 or metal porous plate 3 with a suitable size and a pore diameter of 10 mm.
When the disposable culture dish is separated from the culture medium and the culture thereof by using the device, the method comprises the following steps:
(1) disposable petri dishes to be disposed of are stacked vertically in parallel on a metal mesh plate 2 or a metal multi-well plate 3 in a glass beaker 1, together with culture medium, cultures such as microorganisms, cells, etc. therein, without opening the petri dish lid.
(2) Tap water was poured into the glass beaker 1 and the water level was as low as possible above the height of the disposable petri dish, as shown in fig. 3.
(3) The beaker containing the disposable petri dish soaked in water is placed in an autoclave for sterilization at the temperature of 121 ℃ for 30 minutes.
(4) After the sterilization, the sterilized glass beaker 1 was carefully taken out from the autoclave. The temperature of the contents of the glass beaker 1, immediately after removal from the autoclave, was at 90 ℃. The disposable petri dish therein had amorphous deformations of different degrees, floating in the liquid in the glass beaker 1.
(5) The liquid in the glass beaker 1 was poured off while hot and immediately the deformed disposable petri dish was flushed with a large amount of tap water, while the beaker was filled with tap water, which was at a level higher than the deformed disposable petri dish, and stirred thoroughly.
(6) And (5) repeating the step (5) for 3-5 times to quickly cool the deformed culture dish.
(7) The deformed culture dishes are separated from each other and the cover and the bottom of each culture dish are separated from each other, so that the culture medium contained therein is further dissolved in water.
(8) And further thoroughly cleaning the separated deformed disposable culture dish, picking out other solid impurities such as fungus thalli, cells and the like which are included in the disposable culture dish, obtaining a pure plastic material of the disposable culture dish, and collecting the plastic material after air drying as shown in figure 3, so that the plastic material is convenient for further recovery, processing and utilization.
According to the scheme provided by the invention, whether the culture medium, the culture and the plastic material of the disposable culture dish are suitable to be separated before sterilization is judged in advance. And cleaning the disposable culture dish which is suitable for separation and does not need high-temperature sterilization, and then recovering, wherein the culture medium and the culture enter kitchen garbage after high-temperature sterilization. The remaining disposable petri dishes are sterilized and the plastic material is recovered, wherein the culture medium and the culture enter the sewage treatment route. In contrast, if the disposable culture dish is treated in the usual way, i.e. directly sterilized at high temperature, the plastic material of the deformed disposable culture dish after sterilization tends to coagulate into a large lump, and the culture medium attached thereon is cleaned as much as possible as shown in FIG. 5. In reality, the culture medium in the disposable culture dish melts and flows out when meeting high temperature during sterilization, and the culture medium can be solidified again after cooling, so that a large amount of culture medium can be attached to the plastic material of the culture dish which is usually solidified into a large block. If the plastic materials coagulated into large blocks enter soil, the plastic materials are difficult to degrade, and the growth of crop root systems can be influenced. However, burning plastic materials together with culture media and the like releases a large amount of harmful gases. Therefore, according to the solution provided by the invention, the wastes with different properties in the discarded disposable culture dish can enter a reasonable treatment way.
Claims (5)
1. The disposal method of the experimental waste disposable culture dish comprises the following steps:
if the culture medium and culture in the disposable culture dish to be treated are suitably separated from the disposable culture dish before being autoclaved and the disposable culture dish does not need to be autoclaved, the treatment is performed according to the method 1), otherwise the treatment is performed according to the method 2):
1) opening the cover of the disposable culture dish, and taking out the culture medium and the culture in the disposable culture dish; then cleaning the disposable culture dish to obtain a cleaned disposable culture dish plastic material for recycling;
sterilizing the culture medium and the culture at high temperature, and then putting the culture medium and the culture into kitchen garbage;
2)
s1, vertically and parallelly stacking one or more disposable culture dishes to be processed on a grid plate or a multi-hole plate of a container of the device for separating the disposable culture dishes from the culture medium and the culture in the disposable culture dishes;
s2, injecting water into the container to enable the water level to be over the height of the disposable culture dish, and ensuring that the culture dish can float in the water after sterilization;
s3, placing the container in an autoclave for sterilization; under the sterilization condition, the container and the grid plate or the multi-well plate are not damaged, but the disposable culture dish therein is deformed; after the sterilization is finished, when the temperature of the contents in the container is reduced to 70-100 ℃, taking out the container;
s4, pouring out the liquid in the container while the container is hot, then rapidly washing the deformed disposable culture dish by using a large amount of water, and simultaneously injecting water into the container to enable the water level to be over the height of the disposable culture dish;
s5, repeating the step S43-5 times, wherein the disposable culture dishes are rapidly cooled in the process, and the deformed disposable culture dishes and the cover and the bottom of each single disposable culture dish are further deformed and separated from each other during sterilization; meanwhile, the culture medium included in the culture medium is further dissolved in water; thoroughly cleaning the separated disposable culture dish again, picking out other solid impurities contained in the disposable culture dish to obtain a clean plastic material of the disposable culture dish, and collecting the plastic material after air drying for recycling;
the device for separating the disposable culture dish from the culture medium and the culture in the disposable culture dish comprises the container and the grid plate or the multi-well plate;
the grid plate or the porous plate can be horizontally placed in the container, and a space is arranged between the grid plate or the porous plate and the bottom of the container;
the container is a glass beaker or a barrel-shaped container made of other high-temperature resistant materials.
2. The treatment method according to claim 1, characterized in that: the grid plate or the porous plate is made of metal or other high-temperature-resistant materials;
the aperture of the grid plate or the perforated plate is 4-10 mm;
the grid plate or the porous plate is arranged in the container through a triangular bracket.
3. The disposal method as recited in claim 1 or 2, characterized by: in step S1, the lid of the disposable culture dish does not need to be opened.
4. The treatment method according to any one of claims 1-3, characterized by: in the step 1) or S3, the sterilization temperature is 100-121 ℃, and the time is 10-30 min.
5. The treatment method according to any one of claims 1-4, characterized by: the water is tap water.
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| CN202011180078.8A CN112388868A (en) | 2020-10-29 | 2020-10-29 | Disposal method of disposable culture dish for experiment |
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| CN202011180078.8A CN112388868A (en) | 2020-10-29 | 2020-10-29 | Disposal method of disposable culture dish for experiment |
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2020
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| EP3067072A1 (en) * | 2013-11-05 | 2016-09-14 | Fujifilm Corporation | Sterilization tray and moist heat sterilization method |
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Application publication date: 20210223 |