CN1123838A - Production of yeast with rich ergosterol - Google Patents
Production of yeast with rich ergosterol Download PDFInfo
- Publication number
- CN1123838A CN1123838A CN 95114213 CN95114213A CN1123838A CN 1123838 A CN1123838 A CN 1123838A CN 95114213 CN95114213 CN 95114213 CN 95114213 A CN95114213 A CN 95114213A CN 1123838 A CN1123838 A CN 1123838A
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- Prior art keywords
- yeast
- ergosterol
- remains
- rich
- urea
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The method for producing yeast with rich ergosterol features that yeast strain "E" is used as spawn, which, after multi-stage enlargement culture, is then inoculated in fermenting tank (50 cu.m) for two-stage culturing for 12 hrs. Its ergosterol content is up to 1.4-1.6%.
Description
The invention provides a kind of manufacture method that is rich in ergosterin yeast, belong to zymic zymotechnique technology.
Ergosterol is the polymer monohydroxy-alcohol that a class contains perhydrocyclopentanophenanthrene, is synthesis of vitamin d
2Precursor substance, after UV-irradiation, can be converted into vitamins D
2In industrial production, all from officinal dried yeast, extract ergosterol at present.For improving ergosterol content in the yeast, people adopted several different methods, and as interpolation Yelkin TTS in substratum or employing control yeast growth speed or the like, but in the gained yeast, ergosterol content generally all is difficult to surpass 0.6%.
The objective of the invention is to put forward a kind of manufacture method that is rich in ergosterin yeast of arch, in the gained yeast, ergosterol content can be up to 1.4～1.6%.
The objective of the invention is to realize that by such method it is carbon source with molasses, nutrient media components comprises amount of urea, ammonium sulfate, phosphoric acid etc., and be generally: substratum contains glucose 4% (w/v), ammonium sulfate (NH
4)
2SO
40.2% (w/v), phosphoric acid H
3PO
40.1 (w/v), sal epsom MgSO
40.01% (w/v), PH4.6,28～30 ℃ of culture temperature, bacterial classification is carried out multistage enlarged culturing, be characterised in that and adopt yeast strain " E " as bacterial classification, and will contain glucose 0.5～1.0% (w/v), (NH through the yeast strain " E " of multistage purebred enlarged culturing according to inoculum size 20% (v/v)
4)
2SO
4The molasses hydrolyzed solution of 0.0025% (w/v), urea 0.007% (w/v), PH4.8 is the requirement of substratum, inserts 50 cubic metres of fermentor tanks, more successively according to cultivating altogether 12 hours as the next stage:
(1), in the middle of 0～6 hour, by fed-batch mode, make that glucose concn remains on 0.8～1.0% (w/v) in the fermented liquid, conversion nitrogen content concentration remains on 0.008～0.01%, H
3PO
4Concentration remains on 0.005～0.01%, PH4.2～4.6, and leavening temperature remains on 30～32 ℃, culture volume: the ventilating ratio of volume of air is 1: 2.0～2.5 (v/v).
(2) in back six hours, make fermented liquid keep PH5.2～5.4,36 ℃ of leavening temperatures, ventilating ratio 1: 2.5.
Above-mentioned fermentation finishes, and therefrom collects required resultant---contain the yeast of ergosterol.
It is easy that the present invention has technology, operation is the characteristics of row easily, promptly adopt single-minded barms, carry out substep fermentation control, thereby the intravital ergosterol of gained yeast is accumulated reach the climax within a short period of time, its ergosterol content can reach dry cell weight 1.4～1.6%, exceeds more than one times than well-established law gained ergosterol content 0.6%.
Claims (1)
1, a kind of manufacture method that is rich in ergosterin yeast, with molasses is carbon source, nutrient media components comprises amount of urea, ammonium sulfate, phosphoric acid etc., it is characterized in that adopting yeast strain " E " as bacterial classification, and will contain glucose 0.5～1.0% (w/v), (NH through the yeast strain " E " of multistage purebred enlarged culturing according to inoculum size 20% (v/v)
4)
2SO
40.025 (w/v), the molasses hydrolyzed solution of urea 0.007% (w/v), PH4.8 is the requirement of substratum, inserts 50 cubic metres of fermentor tanks, more successively according to cultivating altogether 12 hours as the next stage:
(1), in the middle of 0～6 hour, by fed-batch mode, make that glucose concn remains on 0.8～1.0% in the fermented liquid, conversion nitrogen content concentration remains on 0.008～0.01%, H
3PO
4Concentration remains on 0.005～0.01%, PH4.2～4.6, and leavening temperature remains on 30～32 ℃, culture volume: the ventilating ratio of volume of air is 1: 2.0～2.5 (v/v).
(2) in back six hours, make fermented liquid keep PH5.2～5.4,36 ℃ of leavening temperatures, ventilating ratio 1: 2.5.
Above-mentioned fermentation finishes, and therefrom collects required resultant---contain the yeast of ergosterol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95114213 CN1123838A (en) | 1995-09-28 | 1995-09-28 | Production of yeast with rich ergosterol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95114213 CN1123838A (en) | 1995-09-28 | 1995-09-28 | Production of yeast with rich ergosterol |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1123838A true CN1123838A (en) | 1996-06-05 |
Family
ID=5080251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 95114213 Pending CN1123838A (en) | 1995-09-28 | 1995-09-28 | Production of yeast with rich ergosterol |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1123838A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100365010C (en) * | 2004-10-25 | 2008-01-30 | 陈国苹 | Ergosterol preparation method |
| CN113980825A (en) * | 2020-07-27 | 2022-01-28 | 安琪酵母股份有限公司 | Strain rich in ergosterol and method for co-producing ergosterol and extract by using same |
-
1995
- 1995-09-28 CN CN 95114213 patent/CN1123838A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100365010C (en) * | 2004-10-25 | 2008-01-30 | 陈国苹 | Ergosterol preparation method |
| CN113980825A (en) * | 2020-07-27 | 2022-01-28 | 安琪酵母股份有限公司 | Strain rich in ergosterol and method for co-producing ergosterol and extract by using same |
| CN113980825B (en) * | 2020-07-27 | 2023-12-12 | 安琪酵母股份有限公司 | Ergosterol-enriched strain and method for co-producing ergosterol and extract thereof |
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Legal Events
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |