CN112359107A - Application and detection method of LINC02085 gene - Google Patents

Application and detection method of LINC02085 gene Download PDF

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CN112359107A
CN112359107A CN202011274190.8A CN202011274190A CN112359107A CN 112359107 A CN112359107 A CN 112359107A CN 202011274190 A CN202011274190 A CN 202011274190A CN 112359107 A CN112359107 A CN 112359107A
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linc02085
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刘健
姜辉
万磊
文建庭
孙艳秋
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First Affiliated Hospital of AHUTCM
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Abstract

An application of LINC02085 gene and a detection method thereof, belonging to the technical field of rheumatism molecular biology. According to the invention, specific PCR primers are designed and synthesized according to the extracted total RNA sequence, and by utilizing the Rt-qPCR technology, the expression of LINC02085 in peripheral blood mononuclear cells of patients with Rheumatoid Arthritis (RA) is found to be remarkably increased, so that the preparation can be applied to preparation of preparations for auxiliary diagnosis, treatment or prognosis evaluation of the rheumatoid arthritis. The invention simultaneously designs and synthesizes pcDNA3.1-LINC02085 specifically over-expressing the gene and si-LINC02085 sequence slightly interfering with the gene, can obviously inhibit the proliferation of synovial Fibroblast (FLS) and inflammatory reaction of the FLS induced by TNF-alpha by regulating PI3K/AKT signal path, and can become a molecular targeted therapeutic tool for RA.

Description

Application and detection method of LINC02085 gene
Technical Field
The invention belongs to the technical field of rheumatism molecular biology, and relates to the fields of expression, application and detection of long-chain non-coding RNA (LINC02085), in particular to application and a detection method of a LINC02085 gene.
Background
Rheumatoid Arthritis (RA) is mainly manifested by symmetrical, peripheral and polyarthritis, has the characteristics of being chronic, progressive and invasive, and can cause autoimmune and inflammatory diseases with systemic multisystem damage. The global incidence of RA is higher, female incidence is higher than male, and synovitis and pannus formation are the basic pathological changes of RA. A great deal of research in the past suggests that the pathogenesis of RA is complex, and the occurrence and development of RA can be closely related to heredity, the surrounding environment, the disturbance of immune function, platelet activation and the like. If the RA patients cannot be diagnosed and treated properly in an early stage, great hidden danger is brought to the patients, joint deformity and function loss can be caused finally, and the emotion, the life quality, the social function and the like of the patients are seriously influenced. In recent years, the development of non-steroidal anti-inflammatory drugs, glucocorticoids, biologicals, and the like have had a great influence on the treatment of RA, but RA has not been cured so far and its pathogenesis is not completely understood. Therefore, the search for biomarkers for early diagnosis, targeted therapy and prognosis evaluation of RA has great significance for the treatment and prognosis of RA.
In recent years, due to the development of high throughput sequencing technologies and various biological information technologies, a growing number of non-coding RNAs (non-coding RNAs), such as circular RNAs (circular RNAs), long non-coding RNAs (long non-coding RNAs), micro RNAs (micro RNAs, mirnas), and the like, play an important role in RA pathogenesis. LncRNA is a regulatory non-coding RNA > 200nt in length, produced by polymerase II transcription, and is an RNA transcript without the function of coding for a protein due to the lack of an open reading frame. LncRNA was originally thought to be a transcribed "noise" or garbage sequence with no biological function, but is now under increasing research with the belief that it may play a regulatory role in whole gene expression, participating in a wide range of physiological and pathological processes in organisms. Most lncRNA consists of a 5 'cap structure and a 3' poly A tail, similar to mRNA encoding a protein. Some studies have shown that lncRNA is involved in RA immunoinflammation, synovial cell proliferation, migration, invasion, and apoptosis, but the mechanism of the same is less studied. The human LINC02085 is located at 3q12.3, and at present, the application of the LINC02085 gene and RA in detection and treatment is not reported.
Disclosure of Invention
Aiming at the defects of lack of diagnosis markers and lack of targeting and pertinence of therapeutic drugs in RA at present, the invention provides application of a LINC02085 gene and a detection method thereof.
The technical scheme adopted by the invention is as follows: an application of a preparation for detecting the expression quantity of a LINC02085 gene in preparation of a rheumatoid arthritis detection preparation, wherein the preparation for detecting the expression quantity of the LINC02085 gene comprises a primer pair with a nucleotide sequence shown as SEQ ID No.1 and SEQ ID No. 2.
Secondly, the preparation for detecting the expression level of the LINC02085 gene further comprises: 5uL of 2 XSSYBR Green mix, 1uL of Forward primer (10uM), 1uL of Reverse primer (10uM), 1uL of cDNA at a concentration of 2.5 ng/. mu.l, 1. mu.l each of SEQ ID NO.1 and SEQ ID NO.2 at a concentration of 10. mu.l/L, and 2uL of RNase Free water.
Thirdly, the invention also provides a detection method of the LINC02085 gene, which comprises the steps of extracting RA Peripheral Blood Mononuclear Cells (PBMCs), extracting total RNA by a TRIzol method, measuring the concentration and purity of the RNA, synthesizing cDNA and amplifying PCR.
Fourthly, the invention also provides application of the LINC02085 gene in preparing a RA targeted therapeutic preparation, and particularly provides a specific overexpression sequence and a small interference RAN sequence SEQ ID NO.3 prepared according to the LINC02085 gene and used for overexpression of the LINC02085 gene in synovial fibroblasts.
Fifth, the present invention provides a diagnostic agent for RA, which comprises a specific over-expression sequence pcDNA3.1-LINC02085 and a small interference sequence si-LINC02085, wherein the nucleotide sequence of the agent is shown in SEQ ID NO. 3. The specific overexpression sequence pcDNA3.1-LINC02085 and the small interference sequence si-LINC02085 are designed and synthesized aiming at the full-length sequence of LINC02085, and can be used for infecting synovial fibroblasts, so that the LINC02085 gene is overexpressed and small-interfered in the cells.
The beneficial effects of the invention are as follows:
according to the invention, specific PCR primers are designed and synthesized according to the extracted total RNA sequence, and by utilizing Rt-qPCR technology, the expression of LINC02085 in peripheral blood mononuclear cells of patients with Rheumatoid Arthritis (RA) is remarkably increased, and the LINC02085 has obvious positive correlation with the age of RA, disease activity indexes ESR, CRP, RF and CCP and negative correlation with IGG, so that the LINC02085 gene can be applied to preparation of auxiliary diagnosis, treatment or prognosis evaluation preparations of the rheumatoid arthritis.
The invention simultaneously designs and synthesizes pcDNA3.1-LINC02085 specifically over-expressing the gene and si-LINC02085 sequence slightly interfering with the gene, can obviously inhibit the proliferation of synovial Fibroblast (FLS) and inflammatory reaction of the FLS induced by TNF-alpha by regulating PI3K/AKT signal path, and can become a molecular targeted therapeutic tool for RA. The invention is expected to further enrich and perfect the research of RA pathogenesis, and also bring hope for developing novel biomarkers for RA early diagnosis, targeted therapy and prognosis evaluation.
Drawings
FIG. 1 is a schematic representation of the expression of LINC02085 in PBMCs from RA patients.
FIG. 2 is a schematic diagram of the correlation analysis of LINC02085 with clinical indicators.
FIG. 3 is a schematic representation of the expression of LINC02085 in FLS.
Fig. 4 is a schematic representation of the effect of LINC02085 on inflammatory factors.
FIG. 5 is a schematic diagram of WB method to detect the effect of LINC02085 on PI3K/AKT pathway.
FIG. 6 is a schematic diagram of the IF method for detecting the effect of LINC02085 on PI3K/AKT pathway.
Detailed Description
The following examples are examples of experimental methods in which specific conditions are not specified, and the tests are usually carried out under conventional conditions or under conditions recommended by the manufacturers.
Example 1
In order to explore the expression of LINC02085 in PBMCs of RA patients.
After the project is examined by ethical committee, the applicant systematically studies new diagnosis and treatment targets of human RA, extracts PBMCs by extracting peripheral blood of RA patients, and detects the expression of LINC02085 gene by RT-qPCR method.
In this example, 30 blood samples of RA patients, 30 blood samples of healthy persons in a control group, and 60 samples in total were collected (the specific information of the samples from 30 patients is shown in Table 1, and the characteristics of the samples are shown in Table 2)
TABLE 1 sample information
Figure BDA0002778610570000031
Figure BDA0002778610570000041
TABLE 2 sample characterization
Figure BDA0002778610570000042
In this example, 30 RA and 30 healthy control PBMCs were detected by RT-qPCR technique, and the specific detection steps were as follows:
1. extraction of PBMCs from RA patients
Adding 6mL of Ficoll-Paque PLUS into a 50mL centrifuge tube at room temperature; adding 4mL of fresh anticoagulated blood and PBS with the same amount into a 10mL centrifuge tube, uniformly mixing, carefully spreading the diluted blood sample on the Ficoll-Paque separating medium, then placing the centrifuge tube in a horizontal centrifuge, and centrifuging for 35min at the temperature of 19 ℃ and 400 g; after centrifugation, the material is divided into 4 layers which are sequentially from top to bottom: absorbing the plasma layer, the monocyte layer, the Ficoll-Paque PLUS and the erythrocyte layer, transferring the monocyte layer to another centrifuge tube, adding PBS with at least 3 times of volume, mixing uniformly, centrifuging at 19 ℃ and 400g for 15min, and discarding the supernatant; adding 6mL of PBS for resuspending the cells, centrifuging for 10min at the temperature of 19 ℃ by 100g, and removing the supernatant; then 1mL of PBS was added to resuspend the cells, and then the cells were transferred to a 1.5mL EP tube, centrifuged at 100g at 19 ℃ for 10min, and the supernatant was discarded and stored in a freezer at-80 ℃.
2. RNA extraction
Adding 1ml of TRIzol into the collected cell sediment at room temperature to crack the cell sediment until the liquid is clear and has no cell mass; adding 0.2mL of chloroform, violently shaking for 15 seconds, and standing for 5 minutes at room temperature; centrifugation was carried out at 12000rpm for 10 minutes at 4 ℃ and the supernatant (about 500ul) was taken and added to another EP tube; adding 0.5mL of precooled isopropanol, mixing the mixture gently and uniformly, and incubating the mixture for 30 minutes on ice; centrifuging at 12000rpm at 4 deg.C for 15min, and discarding the supernatant; 1mL of precooled 75% ethanol was added, centrifuged at 12000rpm at 4 ℃ for 5 minutes, and the supernatant was discarded; washing was repeated 2 times. The RNA precipitate was dried at room temperature, 20-50. mu.L of DEPC water was added, and the mixture was stored at-80 ℃ for further use.
3. Determination of RNA concentration and purity
mu.L of the RNA sample was pipetted into the buffer and the absorbance at 260nm and 280nm was determined. Based on the ratio of OD260/OD280, RNA quality was estimated. The ratio of OD260/OD280 is 1.8-2.0, and can meet the experimental requirements. When OD260/OD280 is less than 1.8, the pollution of protein in the solution is obvious; when OD260/OD280>2.2, it indicates that RNA has been degraded.
4. RT reaction
The reverse cassette PrimeScriptRT reagent Kit (supplied by TaKaRa). The method comprises the following two steps:
the first step is to remove genomic DNA.
Reaction system: add total RNA (1. mu.g), 5 Xg DNA Eraser Buffer 2.0. mu.L, gDNA Eraser 1.0. mu.L, DEPC water make up to 10. mu.L, then PCR instrument 42 ℃ 2 min.
And a second step of reverse transcription.
Reaction system: 10. mu.L of the first-step reaction solution, 1.0. mu.L of PrimeScript RT Enzyme Mix I, 1.0. mu.L of RT Primer Mix, and RNase Free dH2O4.0. mu.L, PrimerScript Buffer 24.0. mu.L, 37 ℃, 15 min; taking out the reaction solution at 85 ℃ for 5s to obtain cDNA, and storing at-80 ℃ for later use.
5. Fluorescent quantitative PCR reaction
Taking out cDNA as a template for fluorescence quantification, wherein the reaction system comprises: 2 × SYBR Green mix 5uL, forward and reverse primers (10uM) each 1uL, template cDNA 1uL, RNase Free water 2uL, PCR amplification reaction is carried out on a fluorescent quantitative PCR instrument under the reaction condition of 95 ℃ for 1min, and then 40 cycles of 95 ℃ for 20s and 60 ℃ for 1min are carried out to obtain the productTo Ct value of each sample, beta-actin is used as internal reference gene, and 2 is adopted as experimental result-△△CTThe method of (3) for analysis.
Each detection index primer
Figure BDA0002778610570000051
The results of the experiment are shown in fig. 1, and it was found that the mRNA expression of LINC02085 was significantly up-regulated (P <0.001) in RA patients compared to healthy controls, with statistical significance for the difference.
Spearman correlation analysis showed that LINC02085mRNA expression levels were significantly positively correlated with RA age, disease activity indicators ESR, CRP, RF, CCP, and negatively correlated with IGG, with the results shown in fig. 2.
Example 2LINC02085 overexpression sequences and Small interference sequences and their uses
This example was designed for the full-length sequence of LINC02085, and synthesized its specific over-expression sequence pcDNA3.1-LINC02085 and small interference sequence si-LINC02085 (the nucleotide sequence is shown in SEQ ID NO. 3), infected FLS, so that LINC02085 gene was over-expressed and small interference in cells, and further applied to regulate the inflammatory response of FLS.
The specific virus packaging and cell infection method is as follows:
(1)293T cells were cultured in 6cm dish to 80-90% confluency, the culture broth was decanted and the cells were washed twice with 3ml PBS; (2) adding 1mL of Trypsin-EDTA solution, mixing uniformly, carefully absorbing pancreatin solution, and standing at 37 ℃ for 3 minutes; (3) adding 2mL of DMEM culture solution containing 10% FBS, and blowing to make the cells form single cell suspension; (4) count on a hemocytometer plate, dilute cells to 3X105cell/mL; (5) press 5x103Inoculating 96-well plate with cell/well concentration, mixing well at 37 deg.C with 5% CO2Culturing for 24 h; (6) the transfection reagent Lipofectamin2000 was used to transfect the overexpression plasmids at the following doses, each dose setting three more wells;
Lipo(uL) 0.2 0.3 0.4
plasmid (ug) 0.2 0.3 0.5
(7) Adding 75 mu L (25 mu L/well x 3well) of serum-free DMEM into a 1.5mL EP tube, adding plasmids with different dosages calculated according to the table, uniformly mixing, taking another 1.5mL EP tube, adding 75 mu L (25 mu L/well x 3well) of serum-free DMEM, adding Lipofectamin2000 with corresponding dosages calculated according to the table, uniformly mixing, standing at room temperature for 5 minutes, mixing the two groups of tubes, standing at room temperature for 20 minutes, sucking out the culture solution in a 96-well plate, and adding 50 mu L of serum-free DMEM culture solution into each well; (8) adding the transfection mixture dropwise into a 96-well plate, uniformly mixing, and incubating for 5 hours in an incubator; (9) transfecting the plasmid group, sucking and discarding the transfection solution, replacing the transfection solution with a complete culture medium, and observing the transfection solution after incubating in an incubator for 24 hours; (10) after 48h, detecting the infection efficiency of 293T by RT-qPCR; (11) after 48h, collecting 293T cell supernatant into a 15ml centrifuge tube, centrifuging at 4 ℃ and 1500rpm for 5min, removing cell precipitate, filtering by a 0.45 mu m filter, transferring to a 1.5ml centrifuge tube, and freezing and storing in a refrigerator at-80 ℃; (12) inoculating FLS of recombinant lentivirus to be infected into a six-well plate, wherein the density is 5-8 multiplied by 105Culture in a well; (13) sucking up the culture solution, adding 1mL of recombinant lentivirus solution and 8 mu Lpolybrene (final concentration is 1 mu g/mu L), and placing the mixture into an incubator for culture; (14) changing the liquid after 4 h; (15) after 24h, the liquid is changed in full amount; (16) after 48h, RT-qPCR is used for detecting the infection efficiency of 293T. This gave a FLS with LINC02085 overexpression and small interference.
After the FLS is cultured in a serum-free medium overnight, 10ng/ml TNF-alpha is added for stimulation respectively, and the expression level of LINC02085mRNA is detected by RT-qPCR, and the result is shown in figure 3, and the TNF-alpha stimulation can improve the LINC02085mRNA expression, the LINC02085mRNA expression is remarkably increased after the LINC02085 is over-expressed, and the LINC02085mRNA expression is remarkably reduced after the LINC02085 is small-interference.
FIG. 4 shows that after FLS is stimulated by TNF-alpha, the expressions of TNF-a and TNFAIP2 are respectively detected by ELISA preparation boxes, compared with the NC group, the expressions of TNF-a and TNFAIP2 in the TNF-a group are increased, compared with the TNF-a group, the expressions of TNF-a and TNFAIP2 in the pcDNA3.1-LINC02085 group are obviously increased, and the expressions of TNF-a and TNFAIP2 in the si-LINC02085 group are obviously reduced.
FIG. 5 shows that after FLS is stimulated by TNF-alpha, the expression of p-AKT and p-PI3K proteins in TNF-a group is reduced compared with NC group, and the expression of p-AKT and p-PI3K proteins in pcDNA3.1-LINC02085 group is obviously reduced and the expression of p-AKT and p-PI3K proteins in si-LINC02085 group is obviously improved compared with TNF-a group.
FIG. 6 shows that after FLS is stimulated by TNF-alpha, the expression of p-AKT and p-PI3K proteins in TNF-a group is reduced compared with NC group, and the expression of p-AKT and p-PI3K proteins in pcDNA3.1-LINC02085 group is obviously reduced and the expression of p-AKT and p-PI3K proteins in si-LINC02085 group is obviously improved compared with TNF-a group.
Thus, LINC02085 inhibits TNF-a mediated inflammatory response of FLS by modulating the PI3K/AKT signaling pathway.
Sequence listing
<110> Anhui TCM university first subsidiary hospital (Anhui province TCM college)
Application and detection method of <120> LINC02085 gene
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 22
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
gagtctgatt ctttcaaggt gg 22
<210> 2
<211> 21
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
tttcattctt ctgtctgtgg c 21
<210> 3
<211> 74306
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 3
agtggaaaac ttactgtcca ttttaaatac acattacttt attagaaaca tttagctctt 60
ttggggggtc aaagaacaca ttgaagcttt gattttgagc tattgttatt tttttcataa 120
agcttccaga agctctgcca tgtaaaaaga aagggagcag ctggactcct ccactggaaa 180
gagggcagca ttcactgcag tcacgtgggc ccaggtggcg tctgggcacc agggtgcctc 240
gccttcagga ccagcttcag agctggtttc ctggtagaga atcagactta cagagaggga 300
aggaaggact ctgtgaaagc tgtatggcat gttaacaaaa tcacttaact agaaatattt 360
tctcaattgc tttccctgac agaaaagtga aattctggaa aacactaatt tgagacactg 420
gacaatgcta caggattgga gaaggctcct cagtctccca tctttccttc tctacagcac 480
actcccacac tattccccac acaacagctg gagaaatcct tctaaaaatg ccacagttag 540
atcatgcaac ctccctattc aaaagaaccc agatcccagg gctttgctta atgttcacga 600
ataaaaccca aaagtcctcc tggactctgg ccagctctca aaaaacccat ctcatgttat 660
tcccctttgg gctctcagct catcgatact gaaagttccc ttgctgtttc tggaatgctc 720
gaatcccact gtcttgtgaa ggcctctaca cttgctgctc cctctgtcat gaacactgct 780
ccccagatgt ttaacatagc tcatctcctg gcttacttag gtctttgctc aaatgtcact 840
ttttcagagg cctgaccacc ctgtcctcaa tccccatctt aacactcact cctgtctgac 900
tgtttatctg actgtttacc aggtgtatat ctctttactg tctaccccac tgaaaatgaa 960
tactgatata tccccaatgc ctggaatggt gtctggtatg tagctagtgt caactatttg 1020
tggaatggat gaatgaatga atgaataagt gagagcaaca aaaaatgaca aatatgatga 1080
aagaaaatca ggtaaatggg tttataaatg ctttttgaga atattccctc tatacagata 1140
attttatgag cagtttatgg gaattgagct ttgcagaaaa aaagagaagg gaaggaaaga 1200
aatgaggcag ggacagagag acagtggtag agatggaaag acagagatgg gctcagtggt 1260
taggagaagt agtggtgtaa ttatttgttc attctagcag gcaaaagatt tagtcaagcc 1320
ccaaatggtc atgtcttaag taaaaatcta tggaacgggc tgggcgtggt ggctcatgcc 1380
tgtaatccca gtactttggg aggccgagga aggcggatca cttgaggtca ggagttcgag 1440
accagcctgg ccaacatggt gaaaccccat ctccactaaa aacacaagaa ttagctgggc 1500
atggtggtgg gcacctgtaa tcccagccac tcgggaggct gaggcaggag aatcacccaa 1560
acccgggagg cagacgttgc agtgagctga gattatgcca ctgcactcca gcctgggcga 1620
cagagcgaga ctccagctca aaaaaaaaaa aaaaaaaaaa attccatgga acgattgtat 1680
gaatgatgtt aggggttttc ctggtcaaca ataaagatgt gaaacagatt ttcaagggac 1740
ttgttaagtg ggtggtgaag gatttgttga catgctcaat gtaggaattg aaaattaatc 1800
aaaagcctta gagaactatt agaggaactg ataagatcac caacagaaga cattgcactc 1860
tatgggggac ttatggggtt ttcaggggta atttgaattg cgtaaaattt tcactttatg 1920
ggctaaccca cctagtcctg gagtaagaca tgactccatt tcaaagggag aaagaaagat 1980
taagatttgt tctagttgta cactcaaatt ggtagggtct ctcattttag gttaaggagg 2040
aaaaaaaaat tggtaaggtc gagagaagat gatgttcacc gggccaggag tggtggttta 2100
tgcctgtaat cccagcactt cgggaggccg aggtgggtgg atcacctgag gtcaggagtt 2160
cgagaccagc ctgaccaaca tggaaaaacc ctgtctctac taaaaataca aaattagcca 2220
ggcgtggcga cacatgcctg taatcccagc taatcgggag gccgaggcag gagaatcgct 2280
tgaacctggg aggtggaggt tgtggagagc caagattgtg ccactgcgtt ccagcctggg 2340
caacaagagt gaaactccgt ctcaaaaaaa aaaaaaaaga aaatatgatg ttcaccctag 2400
aagtagttat gtgtcccacc catcttccca actgataagt atttaggaag gaccttgatt 2460
ttgaccaaaa ccaaactatc ggaagttgtg aaatctaagc acttcttttt tttttttttt 2520
tttttttttt ttttgagacg gagtctcgct ctgtcgccca ggctggagtg cagtggcggg 2580
atctcggctc actgcaagct ccgcctcccg ggttcacgcc attctcctgc ctcagcctcc 2640
caagtagctg ggactacagg cgcccgccac tacgcccggc taattttttg tatttttagt 2700
agagacgggg tttcaccgtt ttagccggga tggtctcgat ctcctgacct cgtgatccgc 2760
ccgcctcggc ctcccaaagt gctgggatta caggcgtgag ccaccgcgcc cggccgaaat 2820
ctaagcactt ctatctggct tatcgtcact tctgtgttac agagaataaa gaggttgagt 2880
acaggctgtc aatttccttg cttgccatat agttggtgat tcattaggat tttccagtga 2940
gcactacatg aactaaattt cttgtcttga tgcatgttgg tgccactgga agccatgacg 3000
tggtttgcat gtgatctgca gcctcagggc tggcctctgc gaaggtttta aaaattcaga 3060
ctcactggtc tgtgcattat ttacacaacc ttagcttgga aggcctgaag tagaaatgtg 3120
tcttcacaac tttgagaagt gacaatagca gtgaagggaa ggagtatgga agctgagata 3180
atgcaagatc acataaaaga tttggtgtgt gtgagaacac acagaaatgc ttttaaaaaa 3240
ttagctaaaa gttgaggtga cggtgaattt gtttctgcat tagtgagata taatttctct 3300
ctctcctttt gtgctatgtg tgtgtgtttt gatgtgtcag ggtggggctg atgaagtcta 3360
atggtgctta ctgtgtgatt ggttcacact aacttgtgtt ctaacccaga gaaattactg 3420
aagctttttt tttttcaaga gaaggacggg aaacagacaa gcagctatgg cattacccac 3480
atggcagagg aagctacctt tatggcatct tcacaaacac tgaaatgtgt tattggtctg 3540
catctatgct tcactccaag atttcctagc tctatttttt ttttgcattc caaagtgaaa 3600
agatgtagcc cacatggcag aggaagctac ctttatggca tcttcacaaa cactgaaatg 3660
tgttattggt ctgcatctat gcttcactcc aagatttcct agctctattt tttttttgca 3720
ttccaaagtg aaaagatgta gccttttgtt aacttacaaa attctatagc tcacacaata 3780
gagtaaaata gtaattaata caggttaggc tgtgaggcta atcactgtgg aaaaattgtg 3840
atcaaatcgc ttaatttcct ctgttatcta ggtcagtgag gaaaataatt tctcccactt 3900
tctttgccct ttgttccaaa ctgtttttag tgactgtgga gagttgcagg ttttatcatt 3960
atagtaaaaa gaggagccat tctgacaatt atcagctaaa ttaaaatgaa tggttaaaaa 4020
cagttcagcc cgagaaatac ataatgcagc ttaatgatag aaaagaccca gccaggctgg 4080
gcacggtggc tcacgcctgt aatcccagca ctttgggagg ccgagtcggg tgcatcacct 4140
gaggtcggga gtttgagacc agcctgacca acacggaaaa accctgtctc tactaaaaat 4200
acaaaattag ctgggcgtgg tggcacttgc ctgtaatccc atctactcag gaggctgagg 4260
caggaaaatt gcttgaaccc aggaggtgga ggttgcggtg agccaagatc gtgtcattgc 4320
actccagcct gggcaacaag agcaaaactc tgtctcaaaa aaaaaaaaaa aaaaaaaaaa 4380
gaaagaaaga aaaagaccca gccagtcttt caaccactgg gattgaaatt tcatggtctc 4440
tgaggatgac tcacttctta gctgtgtcat gagtttcatt ttgaaatgaa atacactgtg 4500
tggtttcatt tgaggcttca tgttgatgca aaaaggaaat gagtccatat tgggaagtta 4560
ggttgaaata catatattaa tgatgaaaaa gtgtcaggct gtcgttcagc catccttact 4620
acttctgttc cggcattacc aggctcttct gtaattgtat aacacctgtt tgtctcccct 4680
acttgtctga gaactcagaa ggcaggaact gggtgaggca agtagaagac actcaatgaa 4740
cagctgttga gtgaaccagg gaacatcatc tctaagggga aggaatcagg gaattttcag 4800
ctccaatctt cctgtgtaat tgttgatgag aaaaatctat attcatcttc actgcattta 4860
cacagttgac aagaacggtc ttatttatag aagccacttt atggtgttat ttttcacgat 4920
atagttacat tctcaaactt gcatgttctc ttgaaaccaa tgagaggggt ggtgagcata 4980
ggaagtgtag tggaaacaaa gtagatgtga tgaaaaatgg cgcaagattc tgggttgatt 5040
ttactcttat ttcaatttac atttacattt tttgagtatg tacaatattc tagactgttt 5100
caaggatact agactttgta tctgatttca aagaatttat actgtcatgg tgaggagggg 5160
gacagaaagg agacttctta agtaaccaaa gaaattagag aacaagaaaa aactgtatgt 5220
aacaaaatat ccaactgaat tgtgaggtgg tacagcttag tctttagagg cacaggcttt 5280
taaatcagac taggactggt actggttcta ccattactag ctctgagatc ctggagaagt 5340
tatgtgacct aatcttattt atttatttgc aaagtgggaa tgacaataat tatatttaca 5400
ctataaggtt atgagattaa ataatattca tgtacactgt atatcccaca aggagtatca 5460
gatcaatgaa tgataatgat aattatataa taattataat cttagtgatt aaaggtgaca 5520
atggaaagga ctaggaaaac agctccagta actgaggcta ttttaaaatt tctgtttcta 5580
atccacatga aatagaatgt tttgctaaaa gatggtattg gttacttgta gacaaattaa 5640
aatcatataa tcgataggct ttgataaaaa acattgagaa cagtgcttgc tttggcaaac 5700
attactaagt gaacatcttg aactttcttc tcaaaagaac acagttaaga gtccagagag 5760
ttgctttatt tctgacaacc aaaagggcta tcttccttgt ccaaattata accatgcagt 5820
ccaagggaaa tacgtgtact tttagagaac aacagctttg attctgggaa aggaaataca 5880
tgctatagag agtagccctg agaaattgtt gaagtttgat aatgttgcaa aactgaattt 5940
ctatttgtga ccaacataaa catttttctt ccctttagaa tatgagctcc agtcttttgg 6000
agaaaatctt tttctcttcc tgatattggc tattcatttt atgttttact gagaatgagg 6060
acagtccacg tgaattctct caatatctct tgaagggtta ccctcttttc cttttttcta 6120
cctcagcaca aggtaacttg cttttttcct tgccaagaac actgctctta attctattac 6180
tgctcacctc ctctcagttt tgctccatcc tttaagccct atctttcttc catcttcaat 6240
ctccaatctc tcctcctctg ccacaaatat ggccagccct ctgtatccta aaataacccc 6300
cctctgaccc tgctgctgta gcaggttact gccttatctt tcttccgttt attaccacac 6360
ttcttctaaa agaatacaaa ttctgctttc acttcctgct tatacattca cttcttaacc 6420
ccttgtaata tggtttctac ctttacccct ctatgaaacc accttctgga aaatcactga 6480
gagtttagtt tcatatccaa caaatctaat ggacttgtct cagtcctcat tctccctgtt 6540
gtctctgcag aaatttcagt ttgttgaatg ttctccttct atagcctctg tgataccata 6600
gcctgctggt tcttttatct ttctggaaat ctctcttcca cctccttcac tagctgcttt 6660
ttctttctac tgctccataa ataaaataaa atcttctcca cattctttgc ctcatgtgac 6720
ttcttatcta ctcacattgc tttacacatg aatttcttca atcttttttc ccccttcatc 6780
tcaaaatctt tctcctgatg attctaaaat ctagcaattt ccaattgctg ttaagacttt 6840
cttacttagg taaccagcca ggattttaaa ctcagtgtat ctaaaatgaa gcccattaac 6900
ttatgttcat caaactccaa actccaactg attgtctcta ttttgttttt ataaatgaca 6960
ccactattaa tgaatcatct ttcacaactc acaagtaatt ttttattcgt ccttacttct 7020
cacaccctgt gcctactaaa ttatggagta aggaagattc tataggcttt gtggtacaca 7080
gggtgggaag agcatctatt ttgctgtcag ataagtctga gttcagatca tggttttgct 7140
acttactacc cttttggtct tgagccagtt agttaagctc accagagtct tgatatcctt 7200
atgtataaag cagggaatat aacattccct acctagccaa ttgagaggat tggatgagag 7260
ctgcacattt tatgtggagt cctaaataaa tactgatctc attcttttcc ctaatcgtgg 7320
ttatgcctct catactcact tttcttttcc attaaatgga ataatatgca tcaaggacct 7380
catgcagttc ctgaaatgca gatgctcatt ccacgttttt tttctcctct cttctccttc 7440
cgttgtggcc tgctcagttc aggactgtgt tgcttcttgt ctggacaatt gttgcggctc 7500
ccgacatggc tcttgctcct gcttcagaca tttggattca ccgcattcac tgctggttgg 7560
tttttcttcc tcatgtgtgc tctgattagg acacccttct gctcagaaga cttcacttat 7620
tcatcatttc caagaaaatg aagtaggaaa acatgtggac caagttatct tagagacata 7680
tgtgccatga ctttcttaca tgtgaggtct attttggcca agtggtacca ttcactgttc 7740
cttcaacatg tccttacttt tctattctat taactttcct cctgggtcta ggattgacaa 7800
cgatgcagtc tcaatctttg aaatctattt ctaccatgat gctttccctg attctttaaa 7860
catatgggat ttctctctgc tttggccccc aaaagacttt gcacctctca tgttcacctt 7920
agttctcatt ttagtggtta tttatttact tgttcacact tacccattcc tcccttcatg 7980
atacaataag gttttgagcc tgtgtctaat taatatatct atatttttaa gtagctagtc 8040
ttgcaggccc tgaaaacctt ttggttcaat taaaatagtt taactttgat atacagacct 8100
atgcttgaaa gtgaaagtct tatagacaac agaaccactg tttatgagaa gagaaagaga 8160
aaagataaga ggaataataa gaaaaaaggg cagcttcctt gggttatgag gtaattattg 8220
gaattcggtc agaaaagtaa gaaatagaaa taagtacgtg tcccaagaga agcagcaata 8280
cctaggatta tgctgacaga gtgaggggga tttaaaagca gtctccattc taatgctttt 8340
taaattctcg gcatagtgta atgattacta ttccaaaaca gaagatatgt tggcaaactg 8400
ccctgtattt gaaaatagag gaaatgccct ggaaagaatg agatgtggta aagagaggaa 8460
atgacaatgt actgaaaaga ctgggctgta agtcatcaca gcggcgccct gaaacccgat 8520
atgtcaggca agctggtggg atcctgggat ccctcatgta acggcactca ttctgacaga 8580
agcagagata aaacagacta agtacacaga ggagtttaag gtttcgtgaa tgtaagtgct 8640
gagtacagaa tggtgctcaa taaaagcttg ttaattgatg gaccacaggt ttgtgacaag 8700
acttctcaga gtctgattct ttcaaggtgg gatataagag taaggtaagc acctcagaaa 8760
atgcctgggg caggtcactc agtgttgctg ggactgatgg tgttggcttg gcttgactag 8820
gatttaataa tacagatgtg cctcaactta tgaaggagtt aagtcccgat aaacccatag 8880
caaacttgaa ataccatgag ttcaaaatgc atttaataca cctaactgac tgaacattat 8940
cgattagcct ggcctacctt actcatgctc tgaacactta cattagccta cagttgggca 9000
aaataatcta acacaaagcc tattttataa taaagtattg aatattacat gtactttatt 9060
gactactgca ctgaaagtga aaaacagaat gattgcatag gtatgcaatc attgaagtat 9120
ggtttctact gaacgcatgt tgctttcaca ccattgttaa gtcgaactaa cacaaatctt 9180
ggacggtttg tagtcatatc ttacccttac ataagccact tatttcacaa acttaaatca 9240
taagtaagaa cttggatatc atccaagaag gcctttgagt agccaaatta agtccgatta 9300
aaaaaaaagt tctttaagat gaaaaatagc agtttaaccc tgaccagtcg tttcttctgg 9360
ttaagacagc acacatctat atggttgaca aagtttcatt tcattataaa cttgtctaaa 9420
aagaatagta ttcaaaacac atttttaagc ataacataga gcatttactg ctccattaga 9480
cacactttga aaatcagaag gcaatctctg agatggtgtg gttttggtga attctgctta 9540
agttgcttgg cattcaggtt ttgtgagggc ttgggctgtg atcgtcatcc ctccaacagc 9600
tttgtgtgtc tgagattgaa tatataccca taactcaggg aaaatttgtt tactcatcat 9660
tttatcatca aatacaccat tataagttta gcaagcaaag accaagaaaa aggttaaatt 9720
aatattgatg ttttaattta aatgcaagat aaaaaaaatc tggttaactt tattctccta 9780
aacagttgtt gatgcttttt gaactctaaa aatgaattat ttgaaatgat gcctttaaag 9840
ctttcctttc tctatttcac tttagtttct acaagtatat aggcttctct ttctaagaag 9900
attcagaaat tatctttgag gaaaacttca agaaatttct ttttggcctc ttaaactggg 9960
cttttcattg aattctggtg ctctttgtta aaatttgcat gattggtttt taaataattc 10020
tgctagcatc gtggtctcat acaattatta ttccaggatt aacgagagtc acaaaacttt 10080
tattaaatat ccaaaatact aagaaaatca ctttcaatca gcatgtgaga acagcctatg 10140
attgccttct gctgatttac tgcttgtttg gcatgtttgg caggatattg caatcttgct 10200
ttccactacc aggttcatgt gggaaaactt aggaactgct ccataactgt cacatctgtc 10260
gccgatttca gatttttctg aatttccctc attatcttct gtattttcac tggtttcaag 10320
tttgctcatc aaacttcctg tttttaacca ctgatccatg ctcccagttg aaaatgtaat 10380
ttgaaactat tcacataaaa aagttaaatg cagtataact ttacaacatt aaaaaaacct 10440
cagaatttgg gcttattttg tgttatggaa gaattcactg tgtttagaga attaagcata 10500
agtatgtatt tgtaacaaaa agtatctggt ctcataaact gcagtgataa tttaactgat 10560
ctgtggggaa ttgggtgtgt gtgcatcatg caaaagttta aaaaaaactc actgtttttt 10620
ttttaaattg tattttattt ttacattaac agataaaatc ccaacttttt tattttattt 10680
atttatttat ttatttattt tagatgtggt ctgtctctgt tgcccaggct ggcctcgaac 10740
tcctgggctt aaacaatcct gcctttctcc tgagtagcag ggactacagg tgtggccatg 10800
gccatgtttt tattaataac tacaccattg atcttaatga gacagatgag acagactcat 10860
taaaagaaac ccaactgtaa agtctctgtg gaattgccca gagattatgt aacctgatag 10920
actagcactc ttcctgccag gtagtgatat taccataacc tattctcgta gatatgtttt 10980
caggttttct tactgtaaga gtcatgttta tactatatga tgtggtccac tgtgattgga 11040
gcagaggtga taacttgatt ggcctggatc aatcagatac tcttctataa ttagggaatt 11100
ggaactaaaa ccttctagtt tagtctagtt acattttatg gtagtcagaa tgctaagatg 11160
tccctaatct cactgtagct ggatatgatg ctaacctgca gctactttag aaaagaaata 11220
ttggtggata acactccttt aataccctct gagacatgca gaaaaatctg ctcctttgca 11280
aaatatgcac tgactactat gtctttttgg gttcttgtat ctactttttg tgcttttctg 11340
acttggaact tttagctctc acttcttgtt gccattttgg tgtttatcca agaggtgaag 11400
aatagtcatg agtctctcca gttctgccca ttctgggtcc atactttcta tactggactt 11460
tctgagcttt ttaattgaag gtataccaca aagacatctg attattgaca gcatactgca 11520
tactgatttt ccaacctacc ttggcagagt tggagccttg cttactttta gttcagttga 11580
gataatactg gaatcttcca aaagaaactt tgacaaaaac tagggatgga gtttggtggg 11640
agtggagggg tgggatggtg gaaaagcata gaaatcaatg aattcctgta gggcagggtt 11700
ctgatctgta ctttatgtcc agtcaagatt cagtttggct aggacagctt tagggcaggt 11760
agcctcttgt tcctggacta actttaaact tctctgtatt gggcatatat atttgaatga 11820
gcacaaaatt ttttcttcta cagaagaaaa aaaggtaagt gggatgtcat caaaatttaa 11880
agttttgtgc ttcaaataac atcaagaatg taaaaagaca acaaatggaa tgagagaaaa 11940
tatttagaaa gcatatctct aataaggaat ttgtattcag aatatataaa gaacttttac 12000
aattaacaat aaaagacaaa taattcaatt taaaattggg agataaattg catagacgtt 12060
tctccaaaga gttacgaatg gtgactcagg acatgaaaag acacttaaca ttgttagaca 12120
ttaaggaaat gcaaatcaaa accactataa cactctcatc cattaggatg gctaaaacag 12180
aaaaaaatca aacaccagac aataacaatg tgtgtcagca tgcatgtttt tttttttttt 12240
ttctggactg cttttttttt tctttttctt taataacact ctgtgtaatt gagccacatg 12300
aagcattagt tatgtttggg tttcattgcc atagcacttt tcagcttatt atgactttta 12360
ccatgtatta tgtgcctcat tgatctttac aaccacgtaa ggttggtgaa atagtacatt 12420
attttctctt ttttgaagat aaagacactc aggctcagag atattttgtg acttggccaa 12480
gtcatcctat acgtggttaa actaggactt aaattcaggt tttctttaca atataccata 12540
ctgcttccta aataatactg gcttatgttt aattgcaatg tttagatggc tttaaaatat 12600
tatattcatg gatatcactt cattttaaac ttcacaacca ccctatgaga tgagtaggcc 12660
aaggaatgtt atattaattt tacagttgaa tatgttgctc agaataatca agttttattt 12720
caagctctgc cacccactta ccttggacag attatataat ttctgaaatt ctaagctttt 12780
ttttatgtgt aaactgaagt ctcctactag atgatctcaa agatccctcc taagcctact 12840
attttgtgat cttatccttt agtatgtcct tccatcatca gcatatgtac tttgaggtga 12900
ttttctggct gtggtgaatg agtttctgta ggatgacctg tactttctta ctggattatt 12960
tctgtaaccc ttatatacat ctttaaaaag catggtcaag atcaggggag caggctttag 13020
ccatataaca gaacagggct caaataacgg aacaatatgg ggtttaaatt tatggaacca 13080
aggctttggt ttcattaact tgggctctga agtaattaaa ctacacatag acattctaga 13140
atgtcataat ttcatttagt tggatgacaa catcctaaca catcagagtt aacttcttat 13200
ggtaaataat gtgcctcaca tttttgtaac aatgagtttc tgctgtacca acactttgca 13260
acctggctgt agaaaaaata tccttaattt gtacgttagt atgaagagtg catcagagga 13320
gactcttttt atttcccatt tactttctgc gttatatatc acttgttttt atttattcac 13380
aatgataata aatagatttt aaaagatatg tttaaagata attcttatgt tttcacattt 13440
cctaggcatc taaaaataaa ataatgaact agatatttaa taaatgtttc tagctaaata 13500
attctgtgat cttttccctt cattgatttt gaagaggtaa atttcagctt tttccaggat 13560
attagttcag gaaaataggt gggtgtgaat gttgcctaac agtttttgag ccatctgcac 13620
aatcttttga taacaatcaa ttaggtttca gattctgtca atatttcccc tacaacccaa 13680
gaaccaatga tcttatggaa aataaaaatt gcccttttcc tttttagcag ttatctttgt 13740
ctctctctgc ccttttctct ctctcctctg ttgaagaaag ctataaatgc actgatagta 13800
ttttcagttc atcaaccatg tgggaaatta tcatccttct actttcttaa gaactgaaag 13860
aagttccctc aaagaaaaga gctctctcag tggttggctg agttaaatac agaaagtcaa 13920
aacatatggg aaaatattga agccttttcc tacaacttcc tagagttgtt ctcatgagaa 13980
atgagagtca gcttcaggac aacttaattt tggctgaatt tttgccatct tcccttccat 14040
gtctctataa aggccaccaa gattcaagag tagtagtatg cctgcctaag atagttcaga 14100
gatcaagctt atcagaagag ataagagtat aaagttttct ttgaaaactg tgaagaacta 14160
gtcaaatgta taatactaaa ttccaactaa cttcttaaaa cggcaatggc tgtttctgag 14220
atgtgtttaa agattagtag ttacacatta cttaataaga tagttaaaac ctatttcatc 14280
attttttttt ttatatgaag tcttgctctg tcacccaggc tggagtgcaa tgcagcaatc 14340
tcagctcact gcaacctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccaa 14400
gtagctggga ttacagacac ccaccatcat gcctggctaa tttttttttg catttttgta 14460
gagacggggt ttcaccatgt tggccaggct ggtcttgaac tccttacctc aggtgatctg 14520
cccgcctcag cctcccacag tgctgggatt acaggtgtga gccaccgtgc ctggcctcat 14580
caaattatat tatgatgatt tattcccatt agaccacatt catttcatga aatgtataca 14640
ttcctctttc ctaataataa gaaataaagt aaaaaatcct ataataaaaa agtaacttaa 14700
atatgcaaag agttacattg gtttttaaaa aatcttttag agggattatg ggccaaaagt 14760
ttaaaatcat gatacaagaa tatgctgaac tagaacaatt gagcctgacc attgtttcac 14820
aatactatgg ggccgtggga agttttataa cttaaacttt caaaagatgg ttttcttatg 14880
gatctcttcc aaaagaacat acttcactga atatagttcc gaactatctt gcttgaactt 14940
tttttttttt tcagaattta caatattttc gtatatattc attactactc tttggtattt 15000
actggttgct tgtgtatata aatatagaaa acattctgct aagcactttc catgtacaat 15060
cctgtgtaat agcagcacaa tctgaatgag gctggtacta ttactaattg caaatgagaa 15120
aactgaggct caggaaagtt aattgacttg tcctaagagt gtacatagca aaaatgtggt 15180
agtggggttt tttttttttt ttctggagag tctgacttaa gggttcctgt taactacttt 15240
tttttttttt attattactt taagttttag ggtacatgtg cacattgtgc aggttagtta 15300
catatgtata catgtgccat gctggtgcgc tgcacccact aactcgtcat ctagcattag 15360
gtgtatctcc cgatgctatc cctcccccaa ccccacaaca gttcccagag tgtgatattc 15420
cccttcctgt gtccatgtga tctcattgtt caattcccac ctatgagtga gaatatgcgg 15480
tgtttggttt tttgtgttct tgcgatagtt tactgagaat gatgatttcc agtttcatcc 15540
atgtccctac aaaggacatg aactcatcat tttttatggc tgcatagtat tccatggtgt 15600
atatgtgcca cattttctta atccagtcta tcattgttgg acatttgggt tggttgcaag 15660
tctttgctat tgtgaataat gccgcaataa acatacgtgt gcatgtgtct ttatagcagc 15720
atatagcgcc tccgtggtat tacttctgcc taacttccga ctaatagtat ttccatttgt 15780
aagatagtct tcatattata gaaccattat ttttatcatc ttgggactta tgctaacagt 15840
tcttatggtg ggccactctg acttttagaa tcatggttgc tacacgaact tgaactcatt 15900
caagcatgtt tgggtatcta attttgggaa gtcccactag tttctcatca gtgcattcaa 15960
acaaactcat tcttcatggg ataaatatgt gaaatctttt gtgtaagtag taactccata 16020
tcaggcccag ataatggcat tttttgaggt ataggcgttt gtacttaaga acggctgtaa 16080
accaatcagt gttactgggt ctttatatta atgttaaaat tccatactgt cattaaaggg 16140
aatttagtgt gtgtcaggca cagctaggac ctgttaacta taatactgtt aactgtagca 16200
ctattttacc tcattctagt attagcaata ctagagaggc agctagtgtt atcatgaggc 16260
tgagtatgtt tctatcagcc tcctagggga aagcaatgga tccaagcctt cttagtttta 16320
tttaaatcaa tatgttgagt gatttatcca atatagctag gcagaataaa tgatagaaat 16380
tgtattttag atacataatt ttcccaataa cttccccaag taatttaaaa aatattcaag 16440
acttagtgac ttgactttct tggttaaata gcattgttga cttatttata tatatattca 16500
cttcattggc catatgtgtg gggattattt ttttagatta gattgtggag gggatactat 16560
ggtgtgccac ccaggtcctc cacttcaata ctgaggtact cattctttca gcacctttag 16620
agtgttacct gttgccgata gcttttaccc aagtcccact ctagggattg tcctcagctt 16680
ccttgcccaa gactattggt atggtttagc tgtgtccaca cccaaatctc atcttgaatt 16740
ttagctccca cacaattccc acctgttgtg ggagggaccc agtgggacgt aattgaatcg 16800
tgtgggcagg tctttcccgt gctattctcg tggtagtgaa taagtctcat gagatctgat 16860
ggttttataa aggggagttt ccctacacaa gtactcttct cttgtctgcc accatgtgag 16920
acatgcgtat caccttcagc catgattgtg aggcctcccc agccacgtgg aactgtgagt 16980
ccactaaatc tctttctttt gtaaattgcc tagtctcagg tatgtcttta taaacagtgt 17040
gaaaacagat gaatacacct atgctccttg ctcagaagca gcctgcaccc atgactatgt 17100
gatacgcaaa atgttccagg ctcctttgcc tccatttgga acaactctga agggctattc 17160
cagcccaccc tgacatcagc tgagaccact gttgtttcta cataatggtt cagtctctcc 17220
tcttgtgcac aatccttttt ttcttcagcc ccttacagat actcctgaga tcactctcca 17280
ataaaatcct catgcaaatc cccaccatag tctcttttct ggggaacatg acctaatata 17340
gctagggaag agttttaatt ccaaaattat ttattcttgc cactcatgca gcattcttct 17400
ggtctatctt cctccttttg acagttgtat actctgggaa atcatttttt ttttttaacc 17460
tgagacagag tcttgctctg tcacccaggc tggagtgtag tggtgccacc agggcccact 17520
gcagcctcaa cctcctggac tcaacagatc ctcccacctc agcctcggga gtagctggga 17580
ctacaggcct gtgccataaa gcctggctaa tttttgtact ttttgtagag acagtgtttt 17640
gccatgttgc ccaggctggt ctcaaactcc taggttcaag tgatccacct acctcagcct 17700
cctaaagtgt taggattaca ggtctgagcc atcctgccct gttgaaatca agcttttgat 17760
aattagccta attacaatgt aagtgggaat gcaaatgcac taaaaataaa atttaagaga 17820
aatttggatt cttgccaata cttcctcagc attaaaaatg attagagatc tttcaatata 17880
ataggacatg acaggaaaat taaagccatt cactcaatat tttgggtgcc tactctacat 17940
aaaaatctat gaaaaattaa agaaaagtaa gatatggctc ctgccctcaa ggaaaagaaa 18000
caaataacta gaaaataaat agataagaga aacattagtt gttttaataa agtattgtga 18060
gttctgatta gaaagcaatt tagctgggat aatcagagcg ggtatccgga aaactttata 18120
ttaagagggc attgcaggaa ggctcagtgt gtctgaggac accttagtca acttagcatt 18180
ttaaacccat taaccaactt tttataaatt cctatatgat acaattgtca aatcttcttg 18240
gcccaatctg agtcagttgg agatgagctg attttgccaa tggtagcgtc agctaccatt 18300
gacgtgcctc cacactccca agtctttcct ttcataagcc acaagatcaa atgaccttct 18360
cagtaactta cattttcagt ttgacaaaag tattttcact ggttccttgt aactctgcat 18420
gggagaaatc atgagaaaat ttaacaagga cttaagtgtg tccacttatg tcgatgaatc 18480
atctgctcct agccctgaac cacttcttcc tgccttgttg gctctgactg cttctactct 18540
gttaataagt ggttttgtct gagttgtgct gaagaaaaag atctggtgag ggtggaatgc 18600
gtcatgggta aatgaaagtg ccaattgaag tgagaacaca gaggtgaatg gagagattga 18660
acccatattc ctttaaattt agacctgaac ttatcatttc aagatggggc ttcaatattt 18720
taagttggaa acagttctga gttaatgctc aggggatcca ataataatct gtcaataaag 18780
tagtaatctt gggagatttt ccctaagtgt aatttctgga ctttgtacat atgtcttatg 18840
gtagatatgt acccaaagca gcctaaccaa actgaccaaa ctggtggatt taccaattat 18900
gttttctttg aagccacaca tagaaacctc tgaatctatt agtggactca attcagctgt 18960
tgtaatttgt caactgaagg aacatatcaa gaaccaagac caacttatct aatccacatg 19020
ctactttagc tctttctttc atttcacctg ggattttatt aaaattgtag ttttggaaac 19080
cagggcagga gcagggcagg ggtaggggag cggggcaggg taggggagtg gggcaggggt 19140
aggggagtgg agcagggaga aaggaagaaa aacctaggat tgaagataga gcttcccttc 19200
attgctcaat ccctgatatc ctgtggcatt ggctcccatt ccttgccatt taattccaga 19260
caatagaacc agtcagacct gcagtgctgt atttcagaca ttgcatccta tgacaaacat 19320
gtcaacagat cataaccatg tggcttctct attctttcct gaagcaatat ttctttatta 19380
ttggagcttg ggaattaaat aaagagaaac tttccaaaga attaaggcat ttaagctgtg 19440
tgttttcaaa gcttattttt tgttctattt ggcagcagga gtgctccaag gatttctgga 19500
taatcagcac catagaaaat gattcctcct ttaaagtttc tactaaagaa tttacagtaa 19560
atttttacat tttctattat atagctccaa ccatctaaag caaaactgtg gtgtattgct 19620
tgtgtttaaa acatgcagtc actggactgg aagcaagctt tatggaaggt catatttctc 19680
gaatgttttt aaatctctgg ggctatcttt tttttaaaag agaaagttaa acactgatat 19740
gataatatta aaagttaatt tttattcagc tcctcttagg taccagattt cattctaagg 19800
gcttcacaag cattaactca tttaatcctc attaaacaca ttagttaaat atttttatta 19860
tcctcatttt ttagcttagg aaactaaggc ctagaaagag taagaaggtt gatcaagatc 19920
tcacagctgg taggtgggag agccaggatt tgtatctagg caggctgctt ctaggactgt 19980
gctacatggc gctacatgat ggcaagctgc cattttatag atgataaaat tggtatctaa 20040
aggcatgaaa taacggccag gcgcggtggc ttatgcctgt aatcccagca ctttgggagg 20100
ccagtgcagg cggatcacaa ggtcaggaga tcaagaccat cctggccaac atggtgaaac 20160
gccatctcta ctgaaagtcc aaaaattagc ttgtcatggt ggcgcatgcc tgtagtccca 20220
gctactcgag aggctgaggc aggagaatcg cttgaaccag ggagtcagag gttgcagtga 20280
gcagagattg cgccactgca ctccagcctg gtgacagagc gagactgtgt ctcaaaacaa 20340
caacaacaat aacaacaaca aaaaaaacct taaataactt gctaagatca tatacctggt 20400
aagtcgtgga gctgggattt gaccccaagt cctttgggcc tctttccaat gcctttgttt 20460
tcatttcaag ttgacttata ggtaaaagtc acagaaactg gaatcataca gtcagaagaa 20520
cagccgaaag gcagtttggg tttttcccat tatgctagac aaacaaaacc caaaaaatgt 20580
gggcatttat tttcatattt ccaaatctaa tataaatata ggctcagcag cagtttttcc 20640
caaccaagcg tctacctttg ataagtcctc attgttcctt cttcccccta gaccccaacc 20700
tccaaaagaa ggggtggagg ccagggagaa atcttgacta taaaagtgaa caattaaacg 20760
tacttggggc tgcactgatg aggaatctcc cccattgctc acctgtctag cgccagtagg 20820
gcatctttga agagagtccc aagtccagtg ttgtagagct gtctcatggg acctcagggt 20880
caacccggtg gttgtagtca ccaacgggct tcagcttgcc ctacagaagc tacctgacat 20940
ctatctgacc cacatctact caaaatcata tcacatgttg acttcttgat aaacatgaag 21000
atgacacgac atgtaaaaat actgacagta agtgaacaaa gcaggatacc cagttgaagt 21060
cctgatcatg atgtgaagtt ctttttgaat tttatgtctt taaggattaa gtgaattaga 21120
gtatcttaag cctctaacag tgtttgggac atagtagata ctaatattct tttctttgct 21180
tgtcttccat tcccttccct gttcatgtaa ataatgatcc tggcaaccaa gaaacatacc 21240
agctgatcaa ttattgagca aatatattta aaatacccac tgtgtgcctg tcattcctag 21300
gctttgtgta gaatactaga gttgttgctg gctagccaag tccttaagaa atatactttt 21360
aaaaattatt ttttaaatca tggtaaaatt tacataacac agaaatgatt atttaaatga 21420
tttaattgta cagttcagtg gcattaagta catttacagt gttgtgcaac catcactatc 21480
catttccaga aattttttat tatcacaaac agaaactctg tacccattac ataatgtctt 21540
cattcttctc ttagtagcac ctggtaacgc tttctgtctc catgaatttg cctgttctgg 21600
gtatttctta ttagtggaat aatacaatat ttgtttcttt gtgtatggct tatttcactt 21660
agcataatgt ttttaaagtt tatccatgtt gcagcatgta tcagaatttc ctttttgttg 21720
agatggggtc ttactctgtc actcaggctg gagtgcagtg acatgatctt ggctcactgc 21780
agcctcaact tcccaagctc aggtgatcct cccacctcag cctcctgagt agctgggact 21840
acaggcatgt gccaccatgc ctggctaatt ttttgtattt ttggtagaga tggggtttca 21900
ccatcttgcc caggctgctc gaactcctga gctcaagcga tctgcccgcc tcggcctccc 21960
aaattgctgg gatttcactg caccaggcca gaatttcctt cctttttaag gctagtatac 22020
tattgtatgt gtttgccaca tttgtttatc catttatctg atgatagaca cttaggtttt 22080
ttctaacttt tgtctattgt gaatagtgct gctatgaata tgggtataca agtatctgag 22140
tccctgcttt caattttttt gggcataaac ctgcaagtgg aattgctgga tcatatggta 22200
attttatttt taactttttg aggaaacgtc gtacattttc catagtggct gtgccatttt 22260
actttcccac cagtgacgcg catgagttcc agtttctaca catccttgcc aacacagctt 22320
gtattttagt aaacgcactt cttgcagtat tttccataaa aaatcaccat cctaatggat 22380
gtaaagtaat atctcattgt ggttttgact tgcatttccc taatgactac ggatattgac 22440
atctttttat gtgcttgttg gccatttgtg tgtcttcttt ggagaaatgt ttattcaaat 22500
cctttgttcc ttcctgaatt ggattgtttt tattttcatt gcagagttgt aggagttctt 22560
tatatattct ggatattaat ctgtagtcag atatatgact tacgaatgtt ttctctccca 22620
tcctgtgggg ggtgttttta aaatctcttg atagtatgcc ttgatgcatg aaagttttta 22680
attttgatgg agtccaattt atctatttct tcttttgttg tctgtatttt tggtatagta 22740
tccaagaaat catcgccaaa ttcaacatcc ttcagaaaca tactttttgt ttgtttattt 22800
tgtaaagaaa agtcttggag ctggtccaaa gggagtaagt tatctacact gttcacagtc 22860
agttacagat caaactcctt gttctgctat ttcctggctt ctcactgctg cacttgacta 22920
gtcttaaaaa aaaaattgaa aaacgtcttg ggggagtaaa ggaggagggc aagggctgaa 22980
aaactgagta ttgtgcttgg tatttgggtg actgaatcaa tagtacccca aacctcagca 23040
tgacacaata tacccaggta acaaatatgc acgtgtacac cctgaatcta aaataaaagt 23100
tgaaattatt taaaaaaaga gaaaaatctc aagggaaata gagaataatt gaggtcaatg 23160
tataataatt tctagttgaa agtagtagat aatgaatata tagttgttca gagaagaggg 23220
aaaacaatcc aagtacctta ataaaatctt aactttacat cagtttacta tgagcaagaa 23280
gaaataaagt gcaagtacgg aagacatagt gcttgcccaa ctaaagagga gttctgggaa 23340
taagccaagc acttggaata agtgacagtt ggataatcca aataaacaat ttggatgtac 23400
ataggatctt gtatatatat ttatttattg cataaacttt tatctcccaa atccaattat 23460
gcttttaaga ataggcaaaa gcttgttgta aggtgatgga ttgatggtga atgggagtga 23520
gaagtaaagc aatttttgca agcaagaaaa tcagttttgc aatctgggag gccccttact 23580
ttatactaaa atatctattt gtatagtttt gttccattga tgagaggaaa atatttgcga 23640
tgacatccat gtgtcctgaa gtttctatgg cacttaccac ttgagatatt atgtcctgtt 23700
gtaaaagaag agaccatgat gtttggttta gaaaagaagg tttccctata tatctagatt 23760
atctatctgt ctgtctgtct atctatctta atgccatgcc aatcacctaa tcaattatct 23820
ttcaattcag agtatgaatt tgcaattaaa ttctgggtag ttccttgaag tggctctggg 23880
agtgttaaga actgtaaaca gagttgctat cctattatcc aaagcctgac tccaaattcc 23940
tatttgttat ataattcttc gttggactgg tgtttaaaca cacattcctt tgtatttatc 24000
ttccatttcc tggagcttaa ttttcttctt ggagcaaatc gatcttcatc tttgggaggg 24060
tccagatcac aaagggtctt gtgtgtctgg tcaagatgtg cagatattat atgtcataga 24120
ttgggaagcc agtgagaaga caggattata ttgatttccc ttcatattcc cttcatattc 24180
caacctcact ggggaaagga gccttatgtt ggatattata atcccataaa gaaacaggga 24240
gaaaggggtt tgagaaagag aaacgtaatc aatactcaca tacaaacatg ttcctatggg 24300
gatctattgt caaaaccctg agatttcttt tgcaatgttt accaagcact cagcccatca 24360
gacattaaac acataggctc atcactcctc tttgattctg gcaggcaggt ttcctaactt 24420
ccaatttatc tttcctatag agcaactggt cagatgctga gtgttgggga ttgggacagg 24480
caatttatat tctaaaatgg aggctttaat acttttaatt ttcccttgaa aagtttcaaa 24540
tgtacagaac aatagagata aaaaattatt aaaataaaat caataattta aaataattaa 24600
atatttaatt taatattaaa atagcagaga aaggaacttt gcgattcctg tcacccatgt 24660
tcaataatta gtaatatttt gccatacttg ctttattact aaatctacat ttttgctggt 24720
cttttttatt attattattt tgattgacaa atcataatta taactattta tgaggtacaa 24780
tgtggtattt tgatatatgt ataaaatgtg aaatgattaa atcaagtaaa ttaacatatc 24840
catcacctca attatgtatc attttttatg gtgagacatt tgaagtttac tctgttattt 24900
tgaaatacat attattgttg actatagtca ccctgctctg caatagatct taaaacctat 24960
tcctcctgtc tgtctgacac tttataccct ttgatcaaca gctccctatt gcctctccca 25020
gccactctct ggtaaccacc attctactct ctacttgtat gagttcaata cttgttagat 25080
tccacacaaa aatgagatca catagtattt gtctttctgt gcctggttta tttcactttt 25140
tgctgaagta ttttaaagca aattttctca tgacctcatg tcattacacc tctacatgct 25200
tcattatgca cctcttaaaa agaaacattt tcttacataa ccatagttat cacatctaac 25260
aatattaaca gtgattcttg ggtaacctct agtatatcca gtctacattt aaatgtccct 25320
gcttgtctta aaaatatatt tgtatagttg atttgttgag ctaggattca aacaaaatca 25380
aaaatatttt ctaagcattt cattatactc cagatattag gttttctctg acaacttctt 25440
taaccccacc ttttcagttt gtattggagt gcttttatgc aggtcataat tttggaagcc 25500
atttctatcc atttcattaa atcagttacc atgtatagta ggctgaataa tggcctctcc 25560
cctctcttcc ccatgatatc caggtcctaa tccctggaac ctgtgactgt taacctacat 25620
ggcaagagag attttgtaga tgtgattaag tcaaaaatct tgagatgggg agattatcct 25680
ggattatatg agtgaacatt aaatgcactt acatgtgtcc ttataagaag gaagtggagg 25740
ttgggtgtgg tggctcatgc ctgtaatccc agcattttgg gaggttgagg tgggcagatc 25800
acctgaggtc aggagttcga gaccagtctg gccaacatga tgaaaacccg tctctactaa 25860
aaatacacaa attagcctgg tgtggtggtg ggtgtctgta atcttagcta cttgggaggc 25920
tgaggcagga gaattgcttg aacccgggag gtggaggttg cagtgagctg agatcatgcc 25980
actgtattcc agccagcgag actctgtctc aaaataaaat aaaataaaat aaaataaaat 26040
aaaataaaat aaaataaata aagagaagga agtgaagaga gatttaatac agaagacaga 26100
agacagaagg ccatgtgaca gaagcagaag gaaacagagt cacagagaga acatgctatg 26160
ctgctggctt tgaggatgga ggaaagggcc agagccaaag aatgcaggtg gctactagaa 26220
acaggaaaag gcaatgcaag ggattctttc cttgagactt cagggagctc tacctacatc 26280
ttaatttcac tccagtgaaa ctgattttag agttctggac tccagaacta taagaaaata 26340
tatttgtgct gttttaagcc accaatctat tagcagtttg ttagagcagc cataggaatc 26400
taatacacca tgggtcaaag atgtctatta agtaggaatt ctattctctg tagaagataa 26460
ttcagagacc agagagctac tggagaggca acatgcagtt gaggaaatgg taatgaactt 26520
ggaactggat tctagactac attctgtcaa agactttgag ccaagttgac ctttctgata 26580
ttcatttccc acatatataa aaatcaaagg aatgggccag gcacagtagc tcacacctgt 26640
aagcccagct gtttgggaag ttgaggcagg aggattgctt gggcctaggg gtttgaggct 26700
gcagtgagcc atgatcatgc cagtgcactc cagcctgggt gacagagtaa gactccgact 26760
caaaaacaaa caagacaaaa cacaaaagtc aaaggaatgg gtgggataaa ttatccttga 26820
gggcccttta aactccctac tctttcatca gaaaaaatca agtaagatgg acaaagcaat 26880
agaacaatga gggcagagga aagcaacata ggacaccaaa ttcttataaa aggcctcaaa 26940
gcttaaggga atgtgtggaa ggacatgggg aaggtcgggg ggagagtaac attgcaaagt 27000
aaccaaaaca atcttattac tttatgttgg tgtgtttgtg ctggtatgga gtgtgtttaa 27060
gtggtgtgga cggaaatgcc tcgggtctta ctgagaggca tcctcctttt cttccctgtt 27120
tcttcactat gtcacagcat acccacaaca gcctgatctg caaatcaaca gtgtaaagag 27180
ctcaaatcca gaggctgaag gcttattgtg ttgactttct catcctagag gtcactgaat 27240
gcagaacttg ggaacttctg gacttcctat gcaacagctt gcttaaacct ggaagggacc 27300
cctggctggg aatagtcaca tttatcaaat ggttaataga tcttctactg ggttaaaagc 27360
tttacattcg aaatcatcct atcttcacaa aaaaatctct cttagagtag ttattggcac 27420
ctctgctgtg ttagtaagga aacgaagttc agcagaagat acgattttac tcaagatgac 27480
acagctggca agtagtcaag tctgaatcct tttctaatat taatttatgg ttataaattt 27540
ctctttaagc acaactttaa ttacacataa taaatttaat gtgtcatatt ttcattatca 27600
ttagttcaaa atgctttcta atttccatta tgtttttttc actaatctgt aggttatttt 27660
gaagtatatt ttaaacttcc aggtgggtga gattttcact tatctgttag ttattaattc 27720
taccttactt gaactgtggt tagaaaacat ttttatgaat actattaaac ttagaaatgt 27780
gtaagacttg cttatggctt tcaatttttg taaatatctg tttatacttg aaaagaatgt 27840
gtattctgaa gttcttaggt gtcctctgtg tgtcagttaa atcaaattat ttgttgtgct 27900
gttcagatct tcaatatctt tagtaagata ttgctaagat tctactaaga tggtggattt 27960
gtccattttc tcttttagtt tggtcatttt tttgccttat atattttgat gctatgttac 28020
taagggtata tgaattatat ttgttatata tgtctatgga ctgaaacttt tatgttttta 28080
aagtatccct cttgcaccct tttcagtaaa tggcatgggg aaaatctgat tgccacagac 28140
agaagaatga aactggatgc ctatttctca caatacacaa aagcgattca agattaaaga 28200
cttaactgta agacttgaaa ctgtaaaaat actagaagaa aatctaggga aaactgttct 28260
ggacattggc ctagaaaaag aatttctgac taagacttca aaagcacagt aaacaaaaat 28320
agacaaacgg gacttaatga aactaaaact cttctgcaga gcaaagaaaa taagaaacag 28380
agtgaacaga caacctgcaa aatgggagat agacaatgga gactaagaag agcaaaggaa 28440
tgacagagag gtggaggatg agaaattact taatgggtac aaagtacatt atttgagtga 28500
tggatgccct aaaagctctg acttgaccat tatgcaatct ctgcatgcaa taaaattgca 28560
cttgcacccc atacatttat ataaattaaa aagtaaaata tccctcttta ccactagtaa 28620
tgattttccc cttaaaatct attttaacta tgtagcagac cctcttaata ttctgctcat 28680
attttcctgt cacttgccat tgctgtgcac actgatggtt ttcctactgc catcaccagt 28740
actccatttg agagctttct ctggtaagct tggttcgtgt gcagggaagg gaggctaaag 28800
gttctgggaa gttgaaacac cagtagcagt cctcatccag tgacagatgg aaactggagg 28860
ataaataact cagcttcgtc atctattggg tgggaccact gtgagggata ttctgtatta 28920
tctctcagag gtcctcagca gggctgactc ccaggtgtcc tttgtggtaa cctgctcact 28980
aatacctggc attggcttct ttctcttcct tttatcaccc tttccccttc ccagttctcc 29040
tattagagct tcttgtgatc actttccaaa tgaactatgt gctatggttt gaatatttgt 29100
cacctccaaa actcatgctc aatttaatcc ctaatgtagg aatattgagt ggtgcagcct 29160
ttaagatgtg attggatcat gaggactctc ccctcatgaa tggattaatt cattcatgga 29220
ttaattgatt aatgaattaa tgagttaatg gattaatggg ttatcattgg aggggaactt 29280
gtggtttata agagagagac ctgagctagc atcttagtat gctcagcccc ctgtctgtgt 29340
gatgctctgc actaccttgg gattgtgtag aaagtcttca ccaacaagaa ggcttttacc 29400
agatatgtcc ccttggcctt gggcttccca gtctcaagaa ctgcaagaaa taaattcctt 29460
ttcttgataa attatcaagt ttcaagtatt tcattataag caacagaaaa tgaatgaata 29520
cactatgtgt tcctaaatat ttgtctgagg tctgtatcag agagaatcac agatagtttt 29580
atgttttagt gtttgcttga catatctttt tctatctttt tatttccaac ctttctatat 29640
cctcttattt tagacatgtc tctagtaagt atatagcatg ttttgttttt tttttaataa 29700
taatacaata atttctcttt taattgaatc atttatttca tttctatttg atgcaatcac 29760
tgataatctt tgttttcttc gctccccttt tgttctttct taacttttgg ttttatattc 29820
atctaatgtt ttcattattc aacttctcct cctatcagct tgatacttac acaccgtctt 29880
actattttag tgattagaga ttaccatagc tatcctcaat ttatcaaaat caactttcct 29940
agttaaagct gtgagtcatt gttcatagct gacatccaca gtggtgtggg tttaattcct 30000
atcagcagct catgtcaact ctttctttgc tttcactaca gttttctttt tacttgggta 30060
taactgacca acaatgttat gggatcctta aaagagagag tagtggtgag aatggtaagg 30120
ttggagataa ggggaggtgt catcatggaa tcaaagaaac agtttttggt ttttggggtc 30180
ttggtgagac acaccttttt ctttgctcct ggcctcccta aaacctccct tctgtctcac 30240
ccttcctgct ctgtctcctg ctcttttttt ctctagctct atggtagttt actagggctg 30300
ctggaatgaa gtaccgtaag cttggtgtct taaacaacag aaatttattg tctcacagag 30360
ctggaggcta gaattctgaa ataaaggtgg cagggccata tttcctccta agattcaatg 30420
gcagaatatt ttcttgcctc ttcctatctt cctagcttct tctggtggtt gccagcaatc 30480
cttgccatat cttggcttgt agacatgtta ttccaatctt tgccctcatc tttatagggc 30540
attcttccct gtgcgtctct atgtctcctc acatggcatt ctcattgtgt gtctgtgttc 30600
tcattttctt cttcttataa gaataccagt tactggattg gggcccaccc taatccagca 30660
tgacctcatc ctaacttgat tacatcttca agatcttatt accaaacaag gtcacattct 30720
gaggttccat gtggacataa attttggggg atgctcttca acccagtaca agctcactta 30780
ctctgtagag tgcagtaaaa gtagcactga cttagaaatt agacattcta agtactaatt 30840
gtgcttgtgc tactaattat ataatacaga ctttggagtt cacaagcagg ggttcaaatc 30900
tcagctttgt catttaacta ccttgagcct cagagtagta aaatcaaatg attttatttt 30960
tgagattcag ctttcaaatt ctagtcactc ctttgggcta tttctttttc ttactctgcc 31020
atgcccatct aatccctttc ctctttaaca acttgacctc ttaattccag ttttcttcct 31080
ccttaattct cctctttatc cttttcttcc cactttagaa aaacattatt tcttttaaat 31140
aatagagaca gggtctcacc gtgttgccca ggctagtctc aaactcctgg gctcaagcag 31200
tcccccaaaa ctctggcctt ctaaagtgct gggattacag gcattagcta ccatgcctgg 31260
cctctcccca gtttttttcc ttacaactag gatgatattc atcctggttt gtccaggaac 31320
aatcccagtt tatgcccatt gtcacagtat aattattgat aatgccccct tctactttca 31380
aagcattctg gtttaatgat gagttaaatg attgccctac ttagagtgtt ttcataacac 31440
ttagacctgc ctctatcact gcatgattca caatgtacta tgttattgta ttttaattgt 31500
tttttggatt tgtctccttg gtgggattta gggtagtcac tgggaatgat tttcttcatt 31560
tctgcagact ccaccatata tacctggcat tgggaggagc tcagtcatgc tgcttggaca 31620
tgggaatgtt tttgaaacct tgcttctttc attttgtgca tagcaggtgt tcaataaatc 31680
atttttaatt gaagtagata gctgtctatt atgaaaatga gaaatgaact tgacaagaac 31740
actgcttctt ctctctgccc tcctttattt gtcatcatta gctcagagat ctcattttac 31800
ctccagattt gtgagtcatt tcttcctctt gcctcaccca tagctatttt aattctttga 31860
aaactggagt tagttatcct tttcaagcct ccagtgagtg ctgcggtgac ctgatttcca 31920
gttgctgggt gcttgctttg ggcaagtgta cctccacgat tgaaatcttc cctccacccc 31980
gagctcctga ggctctctcc actctgtcat ctgtttcaga agcagttttt gtaccacctt 32040
gcttttacct ttgaggccat acccactgtt ggttacttca tcctgtggct atgtctatac 32100
aagtgaaatt aagtgcataa tgaagtcaat ggtgggttta attcaggcca cttttgcctg 32160
acacttcttt cttttgctgt cagctcatca cctcagcacc cccaacacac tacttattgt 32220
ttggaggctg ggatctgcta tgccacagga gtattttctg agccttgtca tttacttcaa 32280
atgatggaag acattttctt tcttcaggaa ttatgagacc taatccacct ggatttagca 32340
aatgggttgt tcttcctgct ggttgctaaa aaccatcacg tattcagttt tcgacaaaca 32400
attgttgaga acttactagg tacaagcaat aataccaaca caattattgt cttcagggag 32460
ttctctagag gctgcgggcg tgcactgctc aaatccactt gcgtgagaag ctcatgagga 32520
gggtagttag ctgacagttt ccagctgcgg cccctctggc tctgcccctg cattcacgct 32580
gacaagtcac agacttctgg ggattcttct agctagtaac tgaatgtggt agttgtatgg 32640
taccatggtc aggctatttc tggctggcat gggactcctc taatgaggag tctttgcttg 32700
aggcctcctg gtcggcctgg ctcagactca gtgctgcaat gccgtctgac tcctactacc 32760
cagtcctcct ccttccctcc tattattgag agccctccct gcccatgctg gttgcccctc 32820
cccaaaaaat ccttcacagg cgtttctccc aagaaatctc ctgcatgact aattttgtct 32880
tggcatcttg ttcgtagaag agttgagctg acacaagttt ccagtccagt attgtagatt 32940
aaacatgtgc acaaagaaca cagcatactc ttcattatat tcctctccaa cattaagtgg 33000
tgagattaaa gtacaacagg gacaggtaat cttagattgc gaaaagaggt atattttata 33060
tttaccatag tctgtcattt ccgagaaggc aaagaccatg accaacataa ttatttgttc 33120
aggtgaacag ttctttgttc acttgtcatg ggtgataagt aaaagcactt aagagaagaa 33180
gaataataat agttgtgtaa ttgtaataat aataataata ataataataa taatttattg 33240
agggccctgt ctctgccagg cattaggtac tttgtgtatc tttttttcca gtcttcataa 33300
caaccaagca aagaaggtat tgctgtcatc ctcattttgc agaagaggaa agtgaaaaat 33360
gaaagaggtt aaatagattg ctcaaggttg tccggacgca gtggctcaca cctgtaatcc 33420
cagcactttg ggaggccgag gtgggaggat cacctgaggt caggagttca agaccagcct 33480
ggccaacatg gtgaaacccc atctctactg aaaatacaaa aattagccag gcatggtggc 33540
gcatgcctgt aatctcagct actcaagagg ctgaggcagg agaattgctt gaacccggga 33600
ggtggacgtt gcagtgaatt gagattgcac cactgtactc cagcctgtgc aacaccacgc 33660
ctggcttaat ttttgtattt ttagtaaaga tggggttttg ccatgtcggc cgggctggtc 33720
tcacactcct gacctcaggt gatctgccca cctctgcctc agattattct ttttcctgtc 33780
ttagaaaaaa aaaaaaaaag attgctcaag gtcaccctga agctagtgag tggctgtgcc 33840
agggtttggg tttgtgaagc tctgatgctc ttgaatttcc catgatacca tgctctgtct 33900
ttagggtgat cttcagactt aggccacatg gtaatcagtc tggggaattt gaggaatatc 33960
cctcccaatg ggctagggag tctatagcct agaccagatc ttagtccatg agttctacat 34020
tcttaatatt tcacagtgta tttttaggaa cttctgaaaa agaagaatga tgtcttcaag 34080
atgggcttct ccttttttat tagatcctgt tgtccagagt tgtagagcta gaaagtaccc 34140
tagaaaaaaa gccagataca aaaagccaat atggtatgat tccatttata tgaaatttcc 34200
agaataggga agtgacttaa tgagggtggg gtttcttttt cgggtaatga aaatgttctg 34260
aaattagata gtagtaatga ttgcacaact ctttgaatat actaaaaacc actgagtaag 34320
tatagtttaa aagagtaaat ataataaata tatatacagt gtattaggct tccaggctct 34380
ctttaagaac tcaaaaactt gagttagata aaacttctta cattacaaat gtttaagcta 34440
aactaaatta tgattcatct ctaggctttt accttacttc tagaggtcga atggctttct 34500
tactctaaaa tgggtgcaca cagctcacac ttccagatta tttctttttt attttttgga 34560
gatggagtct tgctctgttg cccaggctgg agtgcagtgg cgtgatcttg gcttactgca 34620
acctctgcct cctgggttca agtgattctc ctgccttagc cccctgggta gctgggatta 34680
caggcacgtc caccatgccc gagtaatttg tttttatttt tattttttat tattacttta 34740
ggttctaggg tacatgtgca caatgtgcag ctttgttaca taggtataca tgtgccaagt 34800
tggtttgctg cacccatcaa ctcgtcattt acattaggta tttctcctaa tgctatccct 34860
cccccagccc cccaccctac gacaggccct ggtgtatgat gttcccctcc ttgtgtccat 34920
gtgttctcat tgctcagctc ccacttatga gtgaaaacgt ggtgtttggt tttctgtcct 34980
tgtgatattt tgctgagaat gatggtttcc agcttcatcc atgtctctgc aaaaaagaga 35040
catgaactca tcctttttta tggctgcata gtattccagg gtgtatatgt gccacatatt 35100
ctttatccag cctatcattg atgggcattt tggttggatc cctttatttc tttctcttgc 35160
ttgattgccc tggccagaac ttccaatact atgttgaata ggagtgatga gagagggcat 35220
ccttgtcttg tgctggtttt caaagggaat gcttccagtt cttgcccatt cagtatgata 35280
ttggctgtgg atttgtcata aatagctctt attattttga gatacattcc atgaatacct 35340
agtttattga gagtttttag catgaaaggc tgttgaattt tgtcaaaggc cttttctgta 35400
tctattgaga taatcatgcg gtttttgtct ttggttctgt ttatgtgata gattatgttt 35460
attgatttac atatgttgaa ccagccttgc atcccaggga tgaagccgac ttgtttgtgg 35520
tggataagct tttggatgtg ctgctgggtt tggtttgcct gtattttatt gaggactttt 35580
acatcgatgt tcatcaggga tattggccta aacttccctt tttttttgtt ttgcctttgc 35640
caggctttgg tatcatgatg atgctggctt cataaaatga gttagggagg attccctctt 35700
tttctattga ttggaatagt ttcagaagga atggtgccag ctcctctttg tacctctgat 35760
agaatttggc tgtgaatccg tctggtcctg gatttttttg gttggtaggc tattaattac 35820
tgcctcaatt tcagaacctg ttattggtct gttcagatat tcaacttctt cctggtttag 35880
tcttgggagg gtgtatgtgt cgaggaattt atccatttct tttagatttt ctagtttatt 35940
tgcatagaga tgtttatagt attctctgat ggtagtttgt atttctgtgg gatcagtggt 36000
gatatcctct tatcattttg tattgtgtct atttgattct tctctctttt cttctttatt 36060
agtcttgcta gcggtctatc aattttgttg atcttttcaa aaaaccagct cctggattca 36120
ttgatttttt gaagggtttt tgtgtctcta tctcctctag agctctgctc tgatcttagt 36180
tatttcatgt cttctgctag cttttgaatt tgtttgctct tgcttctcta gtagctctta 36240
taattgtgat gttagggtgt caatattaga tctttcctgc tttctcttgt ggacatttag 36300
tgctataaat ttcccactac acactgcttt aaatgtatcc cagagattct ggtacattgt 36360
gtctttgttc tcattggttt caaagaacat ctttatttct gccttcattt cgttatttac 36420
tcagtagtca ttcaggagca ggttggtctg tttccatgta gttgtgtggt tttgagtgag 36480
tttcttaatc ctgatttcta atttgattgc actgcggtct gagacacact ttgttataat 36540
ttctgttctt ttacatttgc tgaggagtgt tttacttcca attatttggt caattttaga 36600
ataagtgcaa tgtggtgctg agaagaatgt attttctgtt gatttggggt ggagagttct 36660
gtagatgtct attaggtctg cttggtccag agctgagttc aagtcctgga tatccttgtt 36720
aattgtttgt ctcattgatc tgtttatatt gacagtgggg tgttaaagtc tcccattatt 36780
attgtgtagg actctaagtc tctttgtagg tctctaagaa cttgctttat gaatctgggt 36840
gctcctgtaa tggatgcata tatatttagg atagttagct ctttttgttg aattgacccc 36900
tttaccatta tgtaataccc ttctttgttt cttttgatct ttgttggttt aaagtctgtt 36960
ttatcagaga ccaggattgc aacctctgct tttttttttt tttttttttt gctttccatt 37020
tgcttggtag atcttcctcc acccttttat tattgagcct atgtgtgtgt ctttgcatgt 37080
gagatgggtc tcctctgaat gcaacacacc aatgggtctt gactctttat ccaatttgcc 37140
agtctgtttc ttttaattgg aggcattcag cccatttaca tttaaggtta atattgttat 37200
gtgtgaattt gatcctgtca ttatgatgtt agctggttat tttgcctgtt aattgatgca 37260
gtttcttcat agtgtcaatg gtctttacaa tttggcatgt gttttcagtg gctggtactg 37320
cttgtccttt ccatgttcag tacttccttc aggagctctt gtaaggcagg cctggtggtg 37380
acaaaatctc tcagagcatt tgcttgtctg taaaggattt tatttctcct tcatttatga 37440
aacttagttt ggatggatat gaaattttgt ggtgaaaatc ctttaagaat gttggatgtt 37500
cgcccccact cttttctggc ttttagggtt tctgcagaga gatctgctgt tagtctgatg 37560
ggcttccctt tgtgggtaac ccgacttttc tctctggctg cccttaacat tttttccttc 37620
atttcaacct tggtgaatct gacaattatg tctcttgggg ttgctcttct tgaggagtat 37680
ctttgtggtg ttctctatat ttcctgaatt tgaatgttgg cctgcctggc taggttgggg 37740
aaattctcct ggataatatc ctgaagagtg tcttctaact tggttccatt ctccccatca 37800
ctttcaggta caccaatcaa acgtagattt ggtcttttca cagagtccca tatttcttgg 37860
aggcttcgtt tgtttctttt cactcttttt ttctctaatc ttgtcttctc tgtttatttc 37920
agtaacttga ccttcaacca ctgacatcct ttcttcctct tgttggaatt ggctattaaa 37980
gcttgtgtgt gcttcacaaa gttctcatac tgtggttttt agctctatca ggtcatttaa 38040
gcttttctca acattggtta ttctagttag ccattcgtct aaccattttt caatgttttt 38100
agcttcctgt gataggtgag aacatgcttc tttagcttga cgaagtttgt tattaccgac 38160
cttctgaaac ctatttctgt caactcatca aactcattct cagagcatgt tgttcccttg 38220
ctggcaagga gttgtgtttc tttggaggag aagaggcgtt ctggtttttg aattttcagc 38280
ctttctgctc tggtttctcc ccatcttcat ggttttatct acctttggtc tttgatgttg 38340
gtgacctatg gatggggttt tggtgtggat gtcttttttg ctgatgttga tgctattcct 38400
ttctgtttgt tagttttcct tccaacaggc aggcacctca gctgcagatc tattggagtt 38460
tgctggaggt ccactccaga tcctgtttgc ctgtgtatca ccagcggacg ctgcagaaca 38520
gcaaatattg ctgcctgatc cttcctctgg aagctttgtc ccagaggggc acccacctgt 38580
atgaggtgtc tgttggtccc tactgggagt tgtctcccag tcaggctaca cggggttcag 38640
ggacccactt gaggaggcag tctgtctgtt atcagagctc gaacaccatg ctgggagaac 38700
cacttctctc ttcagagctg tcaggcaggg acatttaagt ctgcagaagc tgtgctgcct 38760
tttgttcaga tatgccctgc ccccagaggt ggaatctaga gaggcagtag gctttgctga 38820
gctgtggtgg gctccaccca gttcaagctt ctctgccgct ttgtgtacac tgtgagcata 38880
gaaccgcctc aggcctcagc aatagtggat gcccctcccc ctgccaagct ccagcatccc 38940
agattgatgt cagactgctg tgctagcagc gaacaaggct ctgtggccgt gggacccact 39000
gagctaggca tgggagggaa tctcctggtc taccagttgc gaagaccatg gcaaaagcgc 39060
agtatttggg caggagtgta ctgttcctcc aggtacagtc actcacggct tcccttggtt 39120
aggaaaggga aaacccccaa ccccttgtgc ctcccgggtg aagtgacgcc ctgccctgct 39180
tcatcttgcc ctctgtgagc tgcacccact gtccaaccag tcccagtgag atgaaccagg 39240
tacctcagtt ggaaatgcag aaatcaccag tcttctgcgt caatcttgct gggagctgta 39300
gaccagaggt gttcctattc ggctattttg ctatttttat ttatttactt ttttgagaca 39360
gagtctcgct ctgttgccag gctggagtgc agtggtggaa tctcggctca ctgcaacctc 39420
tgccttgcag gtcccagttc aatcaattct cctgcctcag cctcctgagt agctggcatt 39480
acaggtgtgc accaccatac ccagctaatt tttgtatttt tagaggagat ggggtttcac 39540
catgttggcc aagctggtct tgaattcctg accttgtcat ctgcccacct tggcctcccg 39600
aagtgctggg attacaggtg tgaaccacca cgcctggcct aatttttgta tttttagtag 39660
agatggggtt ttgccatgtt ggccaggatg gtctcacact cctgacctca ggtgatccgc 39720
ccacctctgc ctcagagatt attctttttc aaggttttca agtattctgt ttgaatgggg 39780
tgtttaagtc aagtggccaa tcaggttctc aactttggac ttggtcctgg atctagttgt 39840
cagacaccag tgaagctcca gtgactgggg aaaataggaa gacctagaca agactcctgt 39900
tttgataggt ggggcttgag caatttctgc caattgctta acattgatta agacatccct 39960
gcacagggac attaattaag acattcttgc atgtctacca ggaatccagg agctgcgatt 40020
gtactgaaag ctgatactat gcaattagtt ggctcccaca gatgtgggaa cttcctgttc 40080
ttggtggagg tcagagaagc tttgcttttt cctataactc ccttgtccca ccaggtgtag 40140
ggtgactctg cagggctggg ctccagacag ggagtctttc catccatctt caatcactag 40200
ctataggtgc cagttgctgt aaaattcttg aatgagttgg ttgtggtctt tgtgtgtctc 40260
aattgtccta gctgtagaat gaggttaatg gcaatggatc cagatattgt gcttgtgtga 40320
agagaagttt gaagagacct taggatccat tatgtctgac acagacttag agacaggaag 40380
tgacattggc agttgatagc agagctggga ctaaacccag gtctctaaat tcatagtcta 40440
aggtcgtttc cactttgact tattcacaag cagacatgtg atcacagaag aggaaaccaa 40500
aatagagcag ggattgattt acaaacccag aatgttctac ttgctatatt atatctacac 40560
caatccccca atcttgtttc tgtacttttt ttcctctgga tttctctgta ggctgttttc 40620
tagccatttt ggggataatt tttccagata tttctcgtat tgaacctcaa aattcaattt 40680
atttctaatc tattccacaa acacttttaa aagtgtgttc taggcattgt ggtagtagga 40740
gatgaaagat gaatcagacc tagaacctgc ccctgaggca tgtacaatct agaagaggaa 40800
acaggcatca atatttgaca acacacaatc ctacttacta caatgaaaac atgccaaagg 40860
tacagaaaag agaaaatgag tgagtaactt cagggcatgg ggcatgaaag gcagggagat 40920
ggaagtagga ttcacagaag agagtggcat ctaaattggg cttgaaagaa aaacagcaat 40980
tagaataaaa aatttgctga tatgctttta cttattatgc ttctattcaa gcactattta 41040
ttgcatttgg tgtaacaaaa attgtatgac aaatgcttct agaagatgac agatagatag 41100
gtacaagttg gaacttggct ttcatactga aaagctcacc agagaaagtt tagaacttaa 41160
ctcagatact gtttccataa catgccattg ccgaggaggc taattgcaag aagagtgctt 41220
tgcctgtagc cagagaaaag atggatatga ccattatgtt tatttcaagg gcagcaacaa 41280
tattggtaat taaacttttt caagcattta ccatgtgctg tacttttttt ttttcttttt 41340
tttttttgat acggagtctc gctttgtagc ccaggttgga atgcggtgga cgatcttggc 41400
tcactgcaac ctctgccacc tgggttcaag caattctcct gcctcagcct cttgagtagc 41460
tgggattaca ggtgtgtgcc accacaccca gctaattttt gtatttttag tagagatgtg 41520
gtttcaccat gttgggcagg ctggtttcga actcctgacc tcaagtgatc tgcccacctc 41580
ggcctcccaa agtgctggga ttacaggtgt gagccaccgg gcccagccca tgtgctgtat 41640
tttgtatatg ttacaagcat cgtgttattt aattctcata ataaccatat acaaagtagg 41700
taccagtatt actggtggct cagagaggga aagcaataac ttgtctaagg tcagagaact 41760
aaaaaatgac aagccagaat tagaacccag gggtttctaa ctccagggac tgaattttaa 41820
ctcctatcct gacccatggt aggaacagag ttggattgta acccagaatt catctggatc 41880
tgttaaatct ctgcatagaa gtcctagcca tgagaatatg ctaggtccat ggggttagac 41940
tcatgagtga atacttgctg agtccctgct taatactgta tgccctgtaa ggagtgcaga 42000
gaaatgtgag ctgatatcct ggaggatgaa acaaaacaaa gctgtggccg taagagaata 42060
agtgcctggc ctaaggtcac atggctagtg aatgagaccc agaacttgaa tggaggactt 42120
ttaacattga aactagtgct cttttatcta caatccttct agataggctt cgacatttat 42180
gcataggaga gtcggctcct gtaggaccag aacctcaaaa aaatcttaca caacttttag 42240
ggtcaaggtt tattatagtt tcttttctga cctagaacag gcattcacaa aatttataat 42300
agtggtgatg ccagaaaaag atgttttgag actgtgagcc agaaagattt ctaagtctcc 42360
ctttgatgtg accccttctt ttcttttcta ctcttcaatt ctgacccatg aactacttga 42420
tattaaaaaa gtcctgctac ataaacagac acttctcaaa agaagacatt cacatagcca 42480
acaaacatga aaaaaagctc aacatcactg atcattagag aaatgcaaat caaaatcaca 42540
atgagatacc atctcacgcc agtcagaatg gacattatta aaaagtcaag aaacaacaga 42600
tgctggtgag gttgtggaga aacaggaacg cttttacact gttggtggga atgtaaatta 42660
gttcaaccat tgcgggagac agtgtggtga ttcctcaaag atctagaacc agaaacacca 42720
tttgacccag caatcccatt actgagtata tacccaaagg aatataaatc attctgttac 42780
aaagatacct gcacacgtat gttcattgca gcactattca caatagaaga gacacgggct 42840
caactcaaat gcccatcact gatagagtgg ataaagaaaa tgtggtgcat atatatacca 42900
tggaacacta tacagccata aaaaggaatg agttcatgtc ctttgcaggg acatggatga 42960
agctggaagc cattatcccc ggcaaactaa cacaggaaca gaaaaccaaa cactgcatgt 43020
tctccatgtt ctcacttata agtgggagct gaacaataag atcacatgga cccagggagg 43080
ggaacaacat acactgtggc ctgttggggg ttgggtgggg gagggacagt attagaaaaa 43140
atagctaatg catgctgggc tttacaccta ggtgatgggt taacaggtac agtaaaccac 43200
tatggtacat gtttacctat gtaacaaacc tgcacatcct gcacatgtac cctagaacta 43260
aaaattaaga aaaaatgggg aaagaacata aacagatact tctcaaacca agacatacat 43320
atggccagca aacatgaaaa aatgctcagc atcactaatc attagagaaa tgcaaattaa 43380
aaccacagtg agataccatc tcacaccagt cagaatggct attaataaaa attcaaaaaa 43440
caacaaatat tagttaagct gtggagaaat gagaatgctt atatactgcc ggagggaatg 43500
taaattagtt cagccactgt agaaagccat ttggagattt ctcaaagaac ttagaactac 43560
cattccactt agcaatccca tgactgggta tatatcgaaa agaaaataaa ttgttctacc 43620
aaaaagacac atacacttgc atgttcattg cagcaccatt cacaatagca aagacatgga 43680
accaaccaaa atatccatca atagtggact gaataaataa aatgtggtac atatacacta 43740
gccacaaaaa atagaaatac tacacagcca caaaaaagtg aaaatatgtc ctttgcagca 43800
acatgcatgc agctggaggc cattatccta agtgaattaa tacagaaaca gaaaaccaaa 43860
tatcacatat tctcacttac aaatgggagg taaacattgg atgctcatgg acataaaagt 43920
gggaacaaca aacatgggag atttctaata gtggggagag agggaatggg gcaagtgttg 43980
aaaaactaac tattgggtac tatgcttact acttgggtga tgggatcaat catgaccaaa 44040
acctcagcat cacgcaaaat gcccttttaa taaacctgta catgtacccc tgaatctaaa 44100
ataaaacaac attataaata aaataaaaca aaacgagaaa agtcctgctt agctaacttt 44160
tgaaatcatg ctggtacata ttctttccat atttcaacag caatataact ataggatgag 44220
ctaaactagt tttcatatag ggcagagttt aaaatctgtg gtgtaaaaaa tctaaaattg 44280
tatttatctc ctgactttag ggagaaaaat gagtaacagc tctggtcaac aaaaaagagt 44340
aaaaattagt tgaaagataa gctagaaaag gaggttagta ttacacaata ttctggaaag 44400
ctgatgggac aatttgatta atgtgttcag agctgtgaag gcctttgtga aagccatgac 44460
cttatgtggt catgtaacac tatcatgact ggagtgtagc caagaccagc tttaaaatta 44520
ggtgtctgtg atggagggac ttcagaaagg gtttctaaag aaaaccttct ctacccctga 44580
aaccctacta ttagtttcat tctcctgatt ctaaaaaatt tttcgagagt tttctactaa 44640
aacatagttc ctctttaaat tggtagcaca tcatgctctt gcttaaaatt tcaaggctgg 44700
ctaataattc atttaccatt gtattgtgtt atgaattaat agattcaatt cttggactat 44760
caagcaggaa tggcttcaag tatcacttag tccaactctt tcactttata catgagggaa 44820
ttgaggctgc agggggttaa gtgatttgcc caaggtccat tcctagacca ggaatgagaa 44880
ctaaggattt cttattccca ttcatgtttt tggaagtttt ttttttaact tcttttatga 44940
gactacatac atacatacat atatacatac atatatatgt gatatgtata tatatgtgtg 45000
tgttttcttt acaaaaattt ttacaatatt aagccataaa tggtaaaaag attaaaatcc 45060
tctttctgcc tttccactcc cccacaggga tactgttgga accttttctg tacacatatg 45120
tacatgaata catacataac aaaataataa aatatagttt ttaatattac tagagctcac 45180
attgcatgta ttgttctgca gcttgcttta tcttaaaaat gtgtatttta tatacatatc 45240
acatatttaa tacatgtaaa tattgcatat taaatatttt atacgcatat tttatgtaca 45300
cattaaataa ttacatgcta tatatgtata aatccaaaag attgaaaaga agtgaaaagt 45360
ctctcactta ctacttcagt gtctcaatca tgtagttttc cttggagtca accagtgtta 45420
ccagtttctt gtgtgactgc ctacagttat tctttgcata tacaagtctg taaacccctg 45480
ctttcaaaaa acaaactgta gcatactcta catatttatt tgcaccttgt tctttttaaa 45540
cttaatgtgt cttgatgatt atcccatact agcagataaa aagcttcctc tttttctttt 45600
aatagctgta gtagtatagt atgtcagaca gttgatacta aggcatttta taaatagcat 45660
tgtgtagtat tgcattttgt gaatttctca tcctttattt aacctgtcac ctactgattt 45720
agtttgtttt caatattttc ctattgaaaa attgtggcaa taaagagctt tgtatatagg 45780
gcatttcatg catactagag aacatttata gaataaattt ctggaagtgg catttctgga 45840
tcaattgtgt gcatttataa tcttgacagt gaaacaatta aaaatatgtc actctggtat 45900
attgactatt taggttaaag gcacttgaaa aacagtaggg gcaaaaagaa tactctgctg 45960
tttcttaaaa gcaacagatg aaatttccca tgaaaaagat cctcaaaagc tgagaagttg 46020
agaccaagag aattctgtac aacctttttt tttttttaac aaggcttaac tcatctttta 46080
agcctaccaa cacaatttaa ttccttcttt acaaattact gttttttgcc taatttaata 46140
tataagcaac tgactaacta cttctttggg tcttcatttc cttctgaggg agggctcccc 46200
atgccatgtc aaacttaaat aaataaatct gtgtgctttt cttctgttaa tctatcttat 46260
gtaaatttaa ttaccgggcc cagcagggac actaagagga tggaggttaa attttgccat 46320
ccctacaaca gatattgcca aattgccctg catggaggta tggaggctgt atcaatttac 46380
ttccctccag caatgtatgg gagcaacttg tcttcccatg acagtgcata gaaattgttc 46440
tcctcttttt cacaccttct tggtcttcca cagaatggct acaccgttgt ttatttaagc 46500
attctcctta ttgatggaca tttaacttat ttccagtttt tcagttttac aaacagagct 46560
gcactgaacg ttcttgtgca aattttcaaa catctcttta ggtatttttc caaagggcat 46620
gtgtttccaa gaactgaaca gtcttgtgca agtttgcaaa catcttttta gaaaatgcac 46680
aaaagtggaa aacttgtttc aaaagttatg agcacattat actttgatag ataatgtcaa 46740
attgctccat gaataggctg cagcagatca tgtcccagac aaagcataaa gggtatctgt 46800
ttccaaaatt atcagtttac ttaatttttg ccactctgat agttaaaaac ttaactcctg 46860
ttttaatttt catttccctg atactagtga tgtcaagtgt attttcatgt attcatttct 46920
tgaaaaaggt agtcagtaag agcaaagctt ctggagtaat agtgtttggt tgcagactcc 46980
aactgccact tggcgacttc cgtcaaaaca attactggtg ggcaggttac ttaacctctc 47040
tgtgcctcaa gggatgttat gaagacttta tgagataaca attgcaaagt tcttagaaag 47100
gtttctggtt tatattaagt gctcactaaa tgttactctt tttgttgata taattatttg 47160
tatttcctct tctgtgaaaa acaccaattt gtatcctttg atataagaat ggtcggtgtc 47220
ttgttaattc acataccctg ttttatattc tggttattaa gcgttttttc ttttgattta 47280
tctgtatcgc taatattttc ttccagtttg ttggttggtt gcttatcttg tttttgtttt 47340
tggtgttttt taaaaaaaat aatgcagaag gtttaaattt ttacatagtt gtataattag 47400
tcaattaaaa atgaatttta ggttttatga agaactttac tactctaata ttgtaattat 47460
atcctcccat aattctttct aatgttttta atttctttta aatgcctaac tctttaatgc 47520
ctcttagcag gtaagaattt tacttctttt attttgtttt aaacaaacga atagcaaatt 47580
gttccatagt atagctacac cagaatttat ctgattttca tttaatttca ctggagaaaa 47640
tttatattag tgtactctgt gtcctcctat ccggttatgt ggtgattttg ctcatcttta 47700
agtttctttt ctttatttag gtcttacacg tttcttatta attttattcc taggcatttt 47760
atagtttcat tactgctgtg aaaagagaat ctttctttgg atatttttca actcgctata 47820
gttcatagca gaaaggaaca gacttttcta tgctctattt taattaaatt ttctaagata 47880
tgtacattct gagaaaagtg taaaaagtct cccattgttt accgatttgt aattttctta 47940
taattcttgt gaattttgat tgatatattt caaaactatg taaagaacac actactctct 48000
aaatagattt ttttcctttg tcttgattag acattggcta ttaacattgc cacatttgct 48060
ttctttcgct taggatttgc acactgtatc ttttcacatc tgtatttttt aacattttgt 48120
cattttcttt tacatctaaa actagcatgg tttcgtgctt tcttcctaac ttgagcacct 48180
taataggtaa atttagtgcc ttcaaattta ttgtgatttc taacatattt gaacttatct 48240
tgtcatcttc tctctctctc tctccacgtc tctccctttc tcctccctct tttcttcctt 48300
cgtcctttct ttccttcttc attctttctg tccttttctg gttcttcaga gggggtgaga 48360
tgtgtgggaa cttctttctt attgtgtctt ggagaggata gaagtgttgg tggaagctca 48420
taatttgtgt ctgaaggttt tccctacaca gcttctatgc tgatctgcag agttgcacct 48480
tggagcttat ataaaaatag atattggact ctacatcaag cccactgaat ctgctggaca 48540
ggattgatgt gcccgtgttc tgaatccttt cttttgtata taaccctata ttttaagctt 48600
tcccagtgct attacatttt taataagcat ttattatata atgtttaatg cttgtgtaat 48660
tttttattcc actattattc agtattaaat tgtttcctgc ttttatgcta ttataaataa 48720
ttctgatata aacctctaaa tgaattaaag gtttttacat aattaataat atttaatttc 48780
ctaggaaatc ttaaaagtgt gattatttta tcaattgaga ctgaatagtt tgaagactat 48840
atacaaaaat tatattaggc tgggtgaggc agctcacgcc tgtaatccca gcactttgga 48900
aggctgaggt gggtggacca cgaggtcaag agattgagac catcctggcc aacatgtcaa 48960
aaccccatct ctactaaaag tacaaaaatt agctgggtgt ggttctgcgt gcctgtagtc 49020
ccagctactc aggaggctga ggcaggagaa tcgcttgaac ctgggaggca gaggttgcag 49080
tgagccgaga ttgtgccact gcactccagc ctggcaacag ggtgagactc cgtctcaaaa 49140
aaatatatat taatatacaa tattacaagt attatgtatt ttatattaat ataattacta 49200
taaattaaat ataaattaaa tttataaatc attatatttg tataaatatt tacacattat 49260
atattattat aaacatatat tatatatatt taaatatata tttattatat atattttaat 49320
agtctaatgg tttattttat ttttaaaggt aaagtttcac atttattgca gtatttatgt 49380
gttttataat catccaatac atgtaaaagt gtgccacctt tttactggtt ttctatcttt 49440
atgtccactg atgcagtttt tttattaact tttattttgg gttcaagagt acatatgtag 49500
gtttgttata taggtaaaca ggggtttgtt gtacaggtta tttcatcgcc caggtattaa 49560
acctagtacc ctacagttat ttattctgtt cctctccctc ctcccaccct ccaccctcca 49620
ccctccaccc tcaagtagac cccagtgtcc attgctccct cctttgtgtt catgagttct 49680
catcattcag ctcccactta taagtgagaa catgcagtat ttggttttct gttcctgtgt 49740
cagttttcta aggatgatat cctccagtcc atccatgttc ctgcaaagac atgatctcat 49800
tctttttttt ggctgcatag tattccatgg tgtatatata ccacatttcc tttatccaat 49860
ctgtcactga tgagcattta ggttgattcc atgtctttgc tattgtgaac agtgctgcaa 49920
tgaacattca cgtgcatgtg aaagactatg tattacgaga atgatttcta caagctatta 49980
tacaataaga tctagaactt ttatgtgtta ataaaaggac cattttctca aaaagcaaag 50040
gcaggaacaa taaaaataga atataaattt aagagaattt aaaactgaag ataacttaaa 50100
ctatatttct cataattgcc cacatggctt tacttcagag tgatgcttga ttaataaaac 50160
atagtccagc aagcaagaac taattctaaa atactttaga attacttaaa tacaaatact 50220
gaatttgtat ttcttaagtt ctggaagaag atacaccgct cagagccacc cctcttactg 50280
ttctctttca tttctcatcc tcttcttttg caccttgtgg tgcttctctt ttccaacgct 50340
ggagaacttt tcctgcgtcc tttgtgttga atctccctca ccatttcctt ctgattctgt 50400
attacaaaag acagggtgag tctggctatg gcagagagat tctccgagcg cagggttggc 50460
agaagccagg accccatgta aatgggaaaa ggcaggaaaa atgccaggaa cattcccact 50520
taaacaacat gtccctttat acctgggaat ctcatctctg tggctaccta taatcatcca 50580
atgtatgtaa aagtatgcca gcattttaat tggttttctc tatctttatg tgtcactgat 50640
gcagtttaaa aaaattctta ttttaggttc aggagtacac atgaaggttt gttatatagg 50700
taaattcgtg tcacaggggt ttgctgtaca ggttatttca tcacccaggt attaaaccta 50760
gttcccaata gttatttttt gtgcttctct ccctcctccc accttccacc ctcaagtaaa 50820
ccccagtgtc tattgtttcc ttctttatgt cgatgagttc ttatcattta gctcccccca 50880
cttataaggt ttgttatatt gcccagggaa ggcaagatac aaactcacct cttctctgta 50940
gtatgattaa ccttgttata agtttataaa atgcaactca tgggaaggaa agaataaaca 51000
aactgttaat tgttcctgtt agtagctcag acacacctga gccttgtctg attgtccaaa 51060
gagtttaaca cactagagtt acacagagaa taatttgtaa taaaataata aacattgttg 51120
ctattaagca ttgattggtt gtgttaatag tttataaaat gttataattt ctcaggttac 51180
ttttattact atgtatcatt gcactagaac ccagtgtcaa tataattagg aggtctggat 51240
tcaaatccta aattctgatt ttaatgagct ttgtgaccat ctaattttac ctttcttggt 51300
catgtttcct tactttaagt ccaaaaatta gaatccacca tcttaaggtc cctctaaaat 51360
tttattatat tttatattct caaaggacct agtggtccaa ttgcctaacc tgtgcattga 51420
ggttaagtta tcacaaatat tttagcagga aatcctacgg acacagggta tagtgaacaa 51480
gagagtagaa attcatcttc cctaatccca tgttctctag taagatgtgc actttgcttt 51540
gagaaagttg ctcatcatat tcttccagaa agtcatgata aatgcatatc tcagaggctt 51600
agttcaaagc tcttctctgg aagacgtcct cctattagct tcctttgata tagcaaatat 51660
cacagtggat gagaaaaggt ttccctcttc ttccaaccca tggtacaaat ccatacacaa 51720
tagaatgttg aaacccagaa ctctagagaa gatacagaat ctgataggat ttaagttcca 51780
ggcaaacttg ggggtgggtt tgaaataaaa tgcctctgta tgaaacatgg caccatgatt 51840
tctttctaag gctgatgtgg cccaatggct acagatatta ggtcaaccca aatggaattg 51900
agtaagtgat ctctttataa aggtggtgta ttgaaacatc ctcatattac atttataatt 51960
ttatatttgg atccttaagt ggttaactgg gtaggggttg tggtgtgatg aggaatacca 52020
tggaaggaac tgcagaaagt agtaatgggg ccagtatgtg acccccaagg tacatctcct 52080
cctttggaag agacacttcc aggtctttgg ttagggatag caaaatgtta gttgcaataa 52140
aagctggaac gtgtttggtt tggggtttgc cctgggccat tctgtgatct ctctagagca 52200
gccgtaaagc taggaaatag agccaggtgg taaggaccca gtgaggtgag ttctaagaga 52260
cagagagaaa gaagcagagg taaccgggca aatagaacag tcaagatgag tccagaatga 52320
taagccacac ctgctgtggt gacagaagaa tggggaaata aaaagatcag cctgttgggt 52380
gggacgcaca tatttttcaa tgagtcaata aaatgctttt cttggagtgc aggctagata 52440
ttatctagtt agtaacttac tttttaaact taagctgagc ttcttaactc cacctcttca 52500
taccttttgc tttatttatt cactagaatt tccacactta tatatgatca ggaatgtgct 52560
atttactatt agagtctctg gtctcaaaga atttgcaatc acatattaaa ccaaccagaa 52620
caaggagatt tatatacaag tcaagtgctg caatattgta ggggaaggaa ttagtaataa 52680
tccttaaaga cagagagata tcaagtaagg aatacaaaag aaagttagaa tagctgcctt 52740
ccaaaaaccc ttctagttct taacagtata taattttgct tgtttctgtc tttccttttc 52800
aagtcccatt acctcgttta tttattaagc aaacatttaa tatacgcgtt gccatgtttc 52860
aggcattaca tatgcctatt cattttatgt tcataataac cctttgtggt aggtgctgtt 52920
accttcattt tatctgtgag taaatgacag cacagagaag ttaagttact cttaacagct 52980
ggtaagtggc tgagctgaga tttgaaccag agactatctc cagagtctgt ggtcttaacc 53040
gttttatcat gttgcatggt tttgaatcga aatatatatt tgtttacaga aaaaggtaaa 53100
atttgaatga tgaacaaaca cctaattctt attaatttcc tcttggatag aaaaagagtt 53160
cattttctct ctttttaaaa aaacaagagc caatttattc tgagtagttt tggaacctat 53220
atcattttaa tttttcactg ttcaggaaat gaaaacacag gatgagatga gggaatgatt 53280
taggtgtcct ttaggatttt ttccaaagtg caaactatac ttttgtggag ccaaaagggg 53340
ccattcttgt gtacacttgg gctaaagtga ctcttagatc cactgttctc taggatattc 53400
cagtctttcc ctcaccaaac aactactgat gggatgacat ttatgcattc atactatgct 53460
agttgagaga tgggtatacg tgggaatgta ggcttggtac ctgctattaa tgagcttata 53520
atttaattgg ggagacaaca cacacacaca cacacacaca cacacacaca cacacacaca 53580
catacttaat taaccttcac aatcacaata taattcaaga accgaaagct ccagtgggca 53640
aacattatgg ctgggctgtt tggctagaag agtagaaggc atattgaaca tatacagttc 53700
tgcagattct cttggctgat ctgtctctag aaccttaacc tcttccaccc atttgctctg 53760
ccttagctgc tgctgctgcg cctcatgaca caacctcatg acaggtccat gagtgtctag 53820
cacctgagac agaccagttt cttgcctgga gtctccggct tcttccacca ctttgagatt 53880
tgtatattgt atcacacagg ctacgcatga gctgtggctt ccgaagcagc ttcccaaaga 53940
gtccagagaa tacaatctag aagtgtggga aagttgactt ttgacagaga aaaacaggag 54000
ctagaaaaat ttgggcagat aagtattgtc atcccttggt atccatgggg gatttgttcc 54060
tggacctccc ttggatacca aaatccatga atgctcaatg ccccagtata aaatggcata 54120
atattagcat ataacctgta cacatcttcc cacatacttt aaatctctct gtgtgtgctt 54180
acagtactta atatgatata aatgctaagt aaatagttgt catactgtgt tgtttagaga 54240
ataatgacaa gaaaaagtgt acatgttcag tacagatgca attaaaaaat atttttgagt 54300
gggtgcagcg caccagcatg gcacatgtat acatatgtaa ctaacctgca caatgtgcac 54360
atgtacccta aaacttaaag tgtaataaaa caaaaaaaaa agaaaaagaa aaaaagaaaa 54420
aaaaatattt ttgatctaca gttggttgaa ttcacagatg aagaactcaa ggatatggag 54480
gaccaaccac actctttccc ttccttcctc taaaaactac taatgtgaga cacagcttct 54540
ctgatcagtc tgtctagaga catgctgggt agctgagcag gtgacctcca agggagtggc 54600
tgagactctt ccagctcatt gtgaagaaga ggccattgtt atgcattatc tcacattgct 54660
tgatgtcttt ctctgtctca ttgtccttct cctccactct tgttgtccca ggattgtact 54720
tccccaaata aaggaatacc ctgtaatctt tgctccagtt tccgttttct agaggtcttg 54780
gaataaggag gaagaaagga cagtctatgc agagtgtaga atatagaaag gaatgatctg 54840
ggctgaaaac atttatgttg gaggtagcca gcgtcaagtt ttggagggcc atactgaggg 54900
ccatgtagat tctttgaagg cctttgagca gaggagtgac atgatggaaa gtgggattta 54960
ggaaggtaga tcaggtggga atagaaaatt ggagtgctat gaagttggga gaactgcagg 55020
cagaaagact agtaggatga gtatagggcc aagctagaga aatgaaatgg cagatggaga 55080
atcttaacat ggctttaact accttggtga actaggaatc aaggtaattc actagaggtg 55140
agtaagagga agataaagtt gaggggtgga tggggaggag aacttcagtg tactctcctg 55200
aggtcaggtg gcatcatatt gtttcttgct gggcctggct gtgtctaggt cttctgctct 55260
ccatcagtac gaaatctagg gcaactgcag aatttctcgt ttcatgatta gtttgtgtat 55320
tatatgcaaa ttgccaaaag actgatcaga tatttacatg gtctattcaa tttggatttt 55380
gttaactaaa tgctgtgaat ttttttaatt aatttttttt ttgagacaga gtctcgctct 55440
gtgttgccca ggctggagtg cagtggcaca acctcggttc actgcaaact ccgcctcttg 55500
ggttcaagca attctcttgc ctcagcctcc caagtagctg ggattacaag catgtgccac 55560
cacgcctggc taatttttgt atttttagta ggaacgaggt ttctcaccat gttggtcagg 55620
ctggtcttgg actcctgacc tcaagtgatc tgctcacctc agcttcccaa agtgctggga 55680
ttacaggagt gagccacagc aaattaaaat taaaattaat ttgttaattt ttgttaatta 55740
aaactactat cacagaaact gttatttaca gcacttttaa tgaacaaaca gataagccct 55800
gtggttacat agcaattgac ttttcttctg gctgtgaagc cagagtaacc acagaagagt 55860
catttagtca tgacttttgt ctagattttg ctgttcatga gaaggcagaa gtgggctgaa 55920
caagccatat attcattctg gacttgaaat gcagaagtgt gtgctcagtt agtttaatta 55980
aaaaatttct cagttaacat ttaaaaatat ttttgaatat tttaaaaatt ttaagtactt 56040
tatacactgg ccagttattg aatgcttgaa tgtttgttga agttccctgt gttcaagact 56100
gtttgcttcc ctagaggaga aaataattaa tgttctaaaa cacagcatga acatatttta 56160
tatgttgaga tagataggca gcaataaact ataaactaga tgagatagta tatctacaca 56220
gcctctgagt tgagaaaaat taaagaatcc ttaagttaaa cacacacaca tgtgcacgca 56280
cacacacaca cacacatacc cagaaacaca gaaatgggtc ccataaggaa aaaggtaatt 56340
tattgtacat gatgctgtag tttctagaga cttagagtgt tgtctggcta gcgactaaag 56400
taagagtctg gttttgactc ctagttgagc acaggtgcca aggtgggtca tttttcaaag 56460
ggtggtgttc tcatagtggt accctttttg tctctagtgt tgaagtggtg atgttgatgc 56520
ttctgaggag atagtatttt ctcccagccc cgacaactgc gcttgcagag atcaagcctg 56580
acccagagat agtgtctcac agctttttgg ctgtattcat tggtaaagag tcagttgctt 56640
gtcatcagta ataacaagtt tcagtttcat cacaggaaaa tgtgagccag gaagggcatt 56700
tagaaatcaa ccaaagttgg ctgggtgtgg tcgctcatgc ctgtaatccc agtgctttgg 56760
gaggccaagg tgggatgatt gcctgagctt aggagtttga gaccatactg ggtaacatag 56820
tgagactgac tacaaaaaaa aaaaaaaaaa aaaaaaaagg tgagagagag aaatcaacca 56880
aaggggtgtg ggagactgac cattgtcata ggtggaaggc taaacagggt tgtgggagtt 56940
tcagaaatgg gggagcattg tagtttgaag tgatggagga gggccaaaca gaaaaataaa 57000
aaatcacctc agcagggaag cgtgaagtac aataaacata tggatgaaaa cagaacaaga 57060
catgaaaact tatttagttt ttaaatttct gcacacagga gaaagtacgt gctatgagtt 57120
ggctcctttc accataccaa gatgcctatg ctagaagctt tgagtgctgc ttaaaatatt 57180
gctacaaggt catgcagcct ctgaaggagc ttcttaagct ggccctaagt attatgttac 57240
tatctttcta tgctttcttt tttccctgga gaataggttt agtttatgat cccaactcct 57300
acttctctgt agataatgct tgcctctatt atttctccag cccccaattt tcacctgcgt 57360
tcaaacacta tttttagctg ccttcttaga tgccttcatt attatgaact cattcagaaa 57420
tcattgtgtc caagcctgaa tttatcttcc tttaaaacct ccctaagttt ctcccagttt 57480
tatctgtgta atactgaaat agtttaagtc cctaaaaaca ttcattacct catcctatac 57540
aatttccttg aaatagttct ttacttactt tgttttatct tccttcccct ttttactgtc 57600
acctttaaaa cctcacattc tcaagtaata acctcttacc ttatcttcct actgctagat 57660
agagactctc caaacattat ttctactatg aaaccattta ttgctttcca tagcctaaag 57720
gggagaccat ggatcctgag tggcctacag agaggtaggc tcgataagaa ggagatggag 57780
cgtgttataa aggcgtctgt caccacacac atatggattt ctcaagtttc atattgagct 57840
ggctcagtgc tccatcaaag ttggaggcaa tgattgaagt cagagacctg gtgcaaattt 57900
agagacttgg tgcaacccag tcagagacct gggttaagca gcctggtaca aatttcggat 57960
aaatacgtgg tctctgttgt acttatcaca catgaaatgg gaaggtgagg agtaaaaatg 58020
ggattatgcc actgcagggt ggatttgaat tagacaaagg agagacagct gaagagatac 58080
agggctgtga gaacatgtat aagttagttt ttccattcct taaacccaca ctaaggcctt 58140
tcctgacagg catttctccc ttccttgatt tatttttctc cataccagtt attcctacct 58200
gacacattct atatttttct tttgttttgt ctccccccaa gtagaatgaa agctccatca 58260
aggcaggaat tttgtctgtt ttgttcactg ctgtctcccc agttcccaga accataactg 58320
gcacaaggca tatgctcaat atttatgtaa atatttataa atatttgttg aacaaatgaa 58380
tggatacact ctctagcatg acatcttatt tatttattgg taagtctgat cacttctacc 58440
ctcaaacatg ttaaatttat ttcttttcta aagaattcct tgtagaattt tctactttct 58500
cctcagctat ccaaattcca tcctattttg ggttctggtc aggctgcatt attactggga 58560
agctttacct gatggaaagc gggaaacctg aaaacatccc agggcacagt aatcacaagt 58620
ctctcagcat cctggagact cttgcttttt gtatcatttg tctttggaat tatcacatcc 58680
tcagaggctg gtgtgcattg cttttcactg ccttcttctg atgctaatca caccttttct 58740
gaacttgatc ttctcttact ttcagtgtta tactcccaca ggtgttggct ttaggcatgt 58800
gcatttttgc tcattcatgc attatttaat tatttaatac acactcctct aaacactgtg 58860
ggagacagaa ataaattagg cccaatacaa gcccttaagg aaatcatagt ctagtttgat 58920
ccagagtatt aactaattaa gttttaaact ccttgaaggt ggaggcattt aaaaatatat 58980
ttgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttccac catgcctcca ataatgctag 59040
gtacttagag agctcaataa atgtaccaaa tgatggaaga accttagaat tagctggaat 59100
ttagactatt ctaatctaac caccccactg tatgggtgaa gatataaaag gagtggtttg 59160
tttattactt gcctgccaga tagctaacca aataactatg ggagggtctg ggttgagaga 59220
aaccagggtc ctaaactacc aggtcaaata cattattgct aaatctctat accattgtca 59280
gaaacatgct acagaaaacc aggctggttt atgtaaaaat aattgtgtta gtactgatta 59340
aaattctaaa gggctagtat taataaaatg aaatatcttg ggtaaaagtg cttaatattc 59400
cttggatgct ctgtttcttt agaattagag agtacaatgg gagtaaatgc atcaaaacaa 59460
tgtaaaattc tttgtgatca ccagggtgtg accctgcctc tggtaacgta cacagcagtt 59520
acttctggaa ccattgcttg agatatctag aaacactctg gtggccctac cttggctatt 59580
cctggctatg actctctgct ccaatctcct gccttatcct ctttacttct aattagttag 59640
tagacatcct tgggaagtaa gaaactagag cttcagggga cccagcttcc tggctcctcc 59700
tgtgccgtct ttagaatact gtggcagaaa ccccaatgat cttggaaatt tgggtttgca 59760
tatttaatct ctcttctgga tctatgatct tcattctcag caaggatcag ccctcgctca 59820
ctctatgtat tttttcaaat tcctggtggt gtgccagttg gtacatgaag aagggaaggt 59880
ttggtgacag agagggtgat acggtttggc tatgtcccca tacaaaatct catctggaat 59940
tataatcccc ataatcccca catgtcaagg gtgggatcag gtggaggtaa ttggatcatg 60000
ggggtggttt cccctgtgct gttcttgtga cagtgagtga gtctcacaag atctgatggt 60060
tttataagtg tctggcattt cccctgcttg cactcagtgt attctccagc catgtgaaga 60120
aggatgtgtt tgctccccct tcagccatga ttgtaagttt cctgaggtct ccccagccct 60180
gcagaactat gaggcaatta aacctctttc ttttataaat tacccattct tgggcagttc 60240
tttatagcag tgtgagaaag gactaataca gagggcttct tctgaagtag actttaagag 60300
aagagtttag aagtagattt ccccattatt aaaaactgac taattttgag cggtaggtaa 60360
gttacagtac ctctttgact gcatttattt ctgagaatct tgtgtgtaga aaatcaatga 60420
aagacaaaat atatctgtaa atataagttc ataaagtaag gctgggagct gtatgtttgc 60480
cattcaagag tgggattaaa aaactattag ctgaccatag agacctagga gtagcaggtg 60540
aaagagaatt ttgagggagg cactagcaac ccagaggaat gtggtcattt tttttttttt 60600
tttttttttt ttacattttc cctatggtta tcacatactg tgtggcatgt aaagactgag 60660
ttctgcacag agatggagca tttcccagag ctgttaggga gatgaattgc atttatctag 60720
gagaggctgt tacttttcct caatttattc agtgattaga aaatagcttg tggggttttt 60780
gtcttcagag acgcatagtt gttttccatg aacaataacc cactggccag atttggtcta 60840
atgttctgag aggctctggc tgtggcagag acagaaaaac tctaccaccc acaggcatag 60900
gaggctgcac cagatttgaa ggatagaagt caggaacaat taaagggcat gcaggcactg 60960
gagtgaaatg ctgatttgga agcatgattt acatgtgggt gttcccagcc tggcatgtgg 61020
ataggcgtcc taaggctaag tttatccttt catttttgaa gtacactgag tgctagtctc 61080
tgtggatagt tcctttgcag aagagaacat ggcagcagtt gggctggtgg cttgctcata 61140
gccacagagg ccaacccata atcctgggta tgttagtgat ggtggtgaga tgaggggaga 61200
ggcaagtgct ttgtacattg agtacctcac cttccagcat gggatccata gcagcttttg 61260
aacattggtc cctttagaat caaacactgg caatgtaact ttaatcatag ggctatttac 61320
tttttttccg cgctacaatc ttgcaaattt tcaaaaagat tatgtgaaac tgcagttaac 61380
aatctgtaaa ttgtgtctct tcagactaaa ttgttctttg ctgttgatga aaggctgtaa 61440
aagcattctg gtttttagtc cttagatgag ttaaaactga tggtgactct agctaaactg 61500
aaacaaactg tctttatctc atctgacctt ttttaggaga tgtttatgta tctttcttac 61560
attattagag tacatagttt agttggagga caggacaggg gcattcttgt tgcatgttgt 61620
tgattgagaa agaagcaagc aagtggcctt atccttatga tgcttggtga agcaattagc 61680
attgtggaat caatgttata aaaaccggct ccgcataacc tattgcaggc atctaaaaat 61740
ctagattagg tctaacccag acacagtttc aagtaaggag gggggtcata taatatgata 61800
ttgtcactca tccctatcat ttcttattac tgttataaac aggaagattt cgaatctaaa 61860
agtaagactt acttgtttga acctgggatt ctggcccaaa ctatacccac cacatttcca 61920
cagagaaggt tcaagtccct ggatgcctat gccaaatgga ttcccccatt ctcccaagaa 61980
tgcaaatgat gacaaattga ccaaagcttc tggactttcc tctctaaaac agggtcaata 62040
cctgagttaa gacagagaat ttcaagtctg tgaatctcag ctttggttct gcttcttctc 62100
tgcagaggag agaatttatg aagccaaaaa aagtacgatg ccaccccata cagaatcaga 62160
gctgtattta ttttgccctt tggtattcca ttcaacattg agatttgtat ttctccaaat 62220
cagggtggga aaagattttc taaggcacag tgggagaggt gctgattggt aaagagtttg 62280
cggaggtgaa agtagaaaga ttgacttgtg agctgtctcc aagaggaatg aatgcaaaac 62340
atttgatttc cctgtttgtt ttttgttgca gataccttga ctatgaataa tagtttcaat 62400
aaggaagaca gaatgagttc tgacacgatg gttgggagct gtgacaggca gacgaaaaat 62460
ggagccaaat ggcatggagg tgtgtcatca ctgctggatt ttaccttgat ctacattcag 62520
ttatctactt cttttcaaaa tgctgggcat tcattcaaga aacagcatat atgttctgac 62580
tttgaagtaa tggatgaact gtcatgtgca gtatatggaa ataagtttta ttaccttctt 62640
cccacattaa ctcatccatc cattcaatag ccatttattg agaagctcac tctgtgttag 62700
gcacagtggt aagtactata ggttatcaat cacgtatttc ctagaaatta tatagtaatg 62760
aacaacgtgc ttttattttt aaattggctt tggttttatg acacatgata aagccttcaa 62820
acaaggaatc gctgcatgaa tagaaaaggt gaagataatt gtgattaatt aatgagcttt 62880
atagcttttt agatccatat ttcagtggac ctaaaaagga gctgccttat tcactatttg 62940
aaatattgtc ttgttattgc aagccagtta agagtaagat aagacattga ttgactctag 63000
agatgctcta gttgttgctt agaagtaaag gtaaatagtc ttctgtgtgc ctacccttct 63060
gcataaccag cagcaattac agcaactaaa actttctatt tttcagcatg gattttgtgc 63120
ctatcattat ttgctatgtg atgctaccat aatacctgcg taagctcaca gccatgaatg 63180
taggaggtgt aataggaaaa tgagactgtg gatcaaaaaa atattgaaac aggatagaaa 63240
gtcactgatg aaagcataga attacatatt attgggcagg atggtggagg aggagaaaca 63300
aaactgtgac caccagtgaa agaaattatg aataaattgc ctttctgatg gttacaatga 63360
aaggaatgta ggtgatgagg gcctccagtg gaaaggaagc atggtaagca aaaatcagta 63420
ggtgacccat gcccttctag tatctgggaa gggaaaagat caaaacctat gcaactgaag 63480
ccagagtcac tgttagtgga gaggaaagtt agtgtgcagc tgaggggctc gagggaggtt 63540
ttcctggcca cttccaaggt ctagtatatg gagtgccaga gggtgttcta tggaaagaat 63600
gggacagcta cccagtgaag aatgtggctg agtgtgggag aggaccgaat atataatttg 63660
cagcaatgtc tcgggaaagc tgagaggacc cagatcttgt gtgtctgtca gagaaaggaa 63720
gaatgagtca ctagttttag cactaaggtt acccaggaaa ttttaacatc tacttgtaaa 63780
ccgtcactta atgtttgact tatgtgataa tttggaattt tatttaaaca tcttaataaa 63840
actttgttaa taaacttcct agtaggagga tttctcccat attttgaatg tttctcaatg 63900
ctgggaaatg aaaatttcca actggcagta tttcataata ctgtttacct gggaaaatta 63960
gtataacaaa tagagtgatt taagttttat tctggaatta cacatccccc ttctccctgg 64020
aaatgtcccc ttaattgttt acagctgctc aaaactcagt ttcatagaag gttgagcagc 64080
agcatatcct agataagaac aatgtgtcat ggagcaatct aacgtgagct ctaacatgat 64140
ctctcaaacc ttgaagaatc aaagaaagaa ccttagtttg gaataaacag tcaattctca 64200
agtaaagctt ataggcattt gaaaaacact ctggtgctat ttcttcagag gtgcacatta 64260
ataaatgtct tgggccctct tttgactgca ggtgtcatga tgttggaaca gtaacaataa 64320
ctcaaccaac cagggcttgt ctgaatacaa tcctacagca gcaggagctg tggagatata 64380
gaaggcaagg aagaccaggt tgtgagggaa gacgaattta ctcccaggaa tgttgggatt 64440
gagagtaata ttctccaata atggtggaag acagaacaat aaaagcccac cagctccttc 64500
tgcatggtgg gataagagaa aagccaaggg cagaagtgag aaaaataacc atcaccaaac 64560
tgaaaagaaa acctttataa aaaaactgga ttgataaatg acctttagta gtggaaagat 64620
accattccaa tgaagcatga attgaaagga tttcttctga aagctaattt acatttttct 64680
tttttttttt ttcattttca gtgtgtttta gcaggtgtat tgttctacat ggttagtttc 64740
tttacctctt tgagaaaaat gggtcagctt gacacatctg gatgaaagaa tccgttaggt 64800
gattatgatt gaagacagat taagccttgt tatgagggga atatttgggg gcttaccctg 64860
aggtgatatt tgatgattta gctgtgggag aatttccctc ccaaaatcag cgtgactgac 64920
actgaaattg ctacagggga accggacctt tccaagaggt gacaaggaca tttacctggt 64980
acaacacaat ggttctggac tggaagtcta gtcccattga tgaagttccc aaaagatgtc 65040
caggtcctaa tccctggaat ctgtgaatat gtcatcttac acatcaaaag aactttgaaa 65100
atgtgattga ggttaaggac cttggaatga ggagattacc ttggattatc tggttgggtc 65160
caagctaatc acatgagtcc ttaaaggaga agaacctttt ttagctgggt cagagagaga 65220
tgtgatgatg aaaacaggct cagggagatg cactgtaaga agggcttgac cagttgtttt 65280
tgactttgaa gatggaggga agggccagga gccaaggaag gtgggtggct tttggaaggt 65340
ggaaaaagca agggaacatt ctctccagag cctccagaaa ggagctcagc cctgttgaca 65400
tctttgtttt tattttttat ttttagtagc attgaggtct cactatgttg cacaggctgg 65460
tcttgaactc ttgagctcaa gcactcctcc cgccttggct tcccaaagtg ctgggattat 65520
aggcatgtgc caccacatct ggcttcctac tgacatctta actttagtgc agtgagactc 65580
atatttgaat ttctgacctt atattataga accataagac attatagaat tatatagaat 65640
tatagaacca taagacaata actttgtgtt gttttaagat actaaatttt tggtaatttg 65700
ttatggcagc acaataaaac caatatagca ttatattatg ccaatatcat ctctttgttg 65760
actattgcta ttatagtttg ttttatgggt cttgtgtaaa caatcaagct ataatattgg 65820
gttaactccc taaggaaaac ttggaatata gaacattatt ttaaagactg aattaggtaa 65880
atacacctgc cagtgaatac attaacaaat taattaatat attcactctt ttcactcaca 65940
aatacttttt gagcccctat tactagccaa acattttgtt agattctgag aagggtctgc 66000
tagacaacat tgtgactata gttaataaca atatattgta ctcttgaaaa ttactaagag 66060
agtagatttt aagtgttctc accacaaaaa ataagtatgt gaggtaatgc agatgttagt 66120
tagcttgatt tagccattcc ccagtgtata catatttcaa accttcatat tatacatgga 66180
taaatatata caatttttat ttgtcaatta aaagcccctc gatgaataca gtttccattc 66240
tgtccttgag gtggagcttg tagacttggt ggggtccggg gatcaacttt taaacaggca 66300
gttccaaagt gtggagtgga taggcttatt ctctttgttg cttttatgat aggtatttag 66360
aatgcacttt cctctgttaa gttaagccag ccaccattcc ctcattcagt ttccatcctc 66420
catgaatttg ctcacatctg cggtctactg tcactcattc tttccttctc ttcctgaggg 66480
cttgtgtgac ttatgttctt ggctttctct taggaacatg gtgcagcttg gccctgcatt 66540
atccatctgg aaaaacagat cataaaacag tccttctctg tactaagtgc taaagtagga 66600
ggaagggcat aagtcagctc cctgagctat atatccttgt tttcaggagc tgagctagac 66660
acaagcagaa cttgcaaata atttacattt ctttctgctg aggtggttca gaataagttg 66720
caacaaacat gaacaggaat ggtgcagtaa ataaaataat aattattgga ttaaaaatta 66780
ctacccatta ttttctagat tgtaaatcct aagggtgtta gctgattaaa ttggcgaagg 66840
cacatctcac caagtcatca gcttcctaat tatgagagat acatctactc aggcaggtac 66900
ctagacaaag aataactaga ttaattactc aagagtcttt ttatttcttc ctgccttcac 66960
ttgttaacta tactaataga gagatggtat taggcatagg gaaaaataca tctgttacca 67020
agaaaaaatg tctgggagtc ttaaacctgt ggaatcctct gcctgtaaac atctagttga 67080
atatttttgt ctggagggtt ggcctttaat ttttttccct gtgttttaag atgatatact 67140
ttacaaaatg tggtcctgat ttatagatcc agcagtgttt aaattggcca gaaagagagt 67200
gtgagaaatg gatgtactat agctcatttg ttttctgttg gtcttccttt ttcattgctg 67260
tcatggtaca tcacagaggc aagtctggtt tctttggcta acctctgaat aaagggcact 67320
gtggctactg caggggagca tgcagaagtg gtgaatgaat gacgaaaaaa atgcgagttg 67380
agtccattca gggaggtgat ttgtggccat atgataaaat gtgcagatgt taattgcgct 67440
tctgtttctc acgcatgatt accttgttgc tgtacactgc ctgtgtgtgc ctgcagaaag 67500
tacataatag attttgcaga taggaggtac aaagagggtg tagtgtgaga acaaaggcac 67560
tgggagtggg caagtgacag atcagaggaa gactaggtgg caagactggg cagagaaaaa 67620
gtacaatatt caggctagga gggcaggcaa ctacagcttg agaatatcac aaagccccta 67680
aggcaagact atgaacttga tatggggttt cccagtattc caccttgcac atgaataaat 67740
catttcatct attctgaagt ctcaagtatc taaccagaaa tttcatttga aatggaaaag 67800
tcagaaatcg tccccaccct ctaactgatt tgttcatggc agtttctata ctgaagcaac 67860
atgtcaagcc tggcatagat tgcatgggtt gcctaaggag agtgaatgcc caggatccat 67920
ctctgggcca ctcaaaatgt aatcatatcg agacaatgag gagcctataa agagtatcta 67980
ataaaagaaa acacagtgga accagggaaa aatctggaaa gagtggtctc atgaaagcca 68040
aaagaggaga gtttcaaaga gtgaattgtg gactaattgg gaaatggaat aacatgtata 68100
gagaaatgtg tctattggat ttggaaacat gcaacagttt catgtgcatg atttggaagg 68160
aagccagatt agagtgggtt tgtccattaa ctgattcatg atgttcacac atatttcttt 68220
gagtaccgaa catgcatcag atattacttc ggttgttggg ggaatcattg gtgcaaaagc 68280
aagcatgatt ctttacggga tcatggtagc ttatgtggga agacaatttt ccaatcacat 68340
tacttctagg ttcctggcca tctagccaaa ccataggtca cagcccttga actggtatat 68400
aatcaactat ttggctattt ctccttccaa gcaatgaaca gtcaggtgca ctgctcagat 68460
ttctgccccc tgggaaaatt tcctttcact actttcctcc aggaatgttc caaagagggc 68520
ctgttgtact gtagccatcc actttcgagt agtgcctgca tgtcatgtag aaccatctgc 68580
aaaccaggcc caagtcttct ggtcttctgt cagttgatca tagggaactc cccatgaggc 68640
cctaggtgca gtctgggaga gagaagttag catagcagaa gtgggagcca cgggcatttg 68700
ggtcacttct ttatgtaatt tacttatgcc ttcagggtct tcttgggctc catcacttat 68760
attacttcca tttgatgatg gagtactgct gtgcacaccc aactttatgg cttggtaggc 68820
gagataatat ctagtttgta atggggagct cagattgcat ggtaagttgg tggctcataa 68880
gcatcctgtc tccaccaagg cccagtagca ggctaggagc tgtttctcaa aaaaagaggg 68940
tagtcatcca caggggatgg gcaggtcttt gctccaaaat cctaagggct tgtgctgcag 69000
gtgcttgcaa aaggctttaa acagcattcc tatctgtcac tgacacttca agcacaattt 69060
gatctgctgg atcttatggt taaagtgcca gagcagcttg catggcagcc tggacctgtt 69120
gtagagcttt ctcttgttct gagccctttt caaactggct gcttttttgg gtcactcagc 69180
aaatgagcca gagtaacaca gtcaaaagaa aaatatgttg ccttcgaatt caaagaggct 69240
cactagttgt tttgtcacct ttccggtcat acaagggtcc agttgcaaca acataccctt 69300
caccttagaa gggctatctc aacttgccac acaccactgg aaccctagaa acttcactga 69360
ggtagatacc tgaatttttc ttggatttat ttccctccct ctgacactca aatgtcttac 69420
caagatatct agagttgtag ctacttcttg gtcgctagac cccattagca taaagtcatc 69480
aatgtgtttg gtcagtgaaa taaaagggaa gggaagagtg gaagagaaga gtgtgattaa 69540
gaatcttgca aacagctggg catggtcatt catgcctgta atcacagcat tttgggaggc 69600
caaggcgggc tgatcacttg aggccaggag ttcaagatca gcctggccaa tacagtgaaa 69660
acctgcctct actaaaaata caggaaatta gctgggtgtg gtggtgcaca tctgtaatcc 69720
cagctacttg ggaggctgag gcacaacaat tgcttgaacc tgggaagtgg aggttgcagt 69780
gagccaaggt tgcaccactg tactccagcc tgggtgacag ggtgagactc tggtctgaaa 69840
aggaaaaaaa aaaaaagatt cctgcaaatg aaattatgat atagggaaga agagttgata 69900
tatccgtgag gtgggacagt gaagatgtac tgcaggctct gccagctgaa agcatactgc 69960
ttctggtggt ctttactaat gggtatcaag aaataccagg aaaatacaag gtgtcagggg 70020
atgtgttggt gtgttcaagt tatgaaacca cacctggtat agcagctgta gttagagtca 70080
ctgtactaat ctcacaatga tctgtcattc atctgtcttt tacaaaggcc aaataggtga 70140
gttgaatgag gatatggtgg gaatcaccac ccctgaatct ttcaagtcct taatggtgga 70200
ctgatctctg caatccctcc aggaatgtgg tattgctttt ggttactatt ttcttagata 70260
gaggcagttt tagttgcttc catctgtccc tttctactgt agtagccctt acaccatagg 70320
tcaatgaacc aatgtgggga ggctgtcacc tgatgagtat atctactcca attatacatt 70380
ctggaactgg gaaaataact acaggatagg ttcaggggca taccaggcca ctgtgatata 70440
gacatgagtt aaaactccat tgattagcag accgccataa gccccttctc tgactgatga 70500
aacacagtga tgtttgaagt gtcctggaat tagtgtcagt tcagagccag tgtccagtag 70560
tccctgaaag gtctgatttc cccctcccct cagtacatac ttacctttgt aaaagactat 70620
agatctcttt gggagaaaga ttaacagtct aaatatttgg cagtgtgcta tgtccttcct 70680
caaaagggga accagcttcc cctttattca agggggggcc ttgatctgta aattggctca 70740
tcagaaggtt aattgagggc ctgtgcctcc tttgtttttt tgattcactt tagaattcgg 70800
ttcacttgac ctagatcttt tctgcttaca tagatgaagt aagaatttag taggcttctt 70860
atctctttag cttgtaggaa caccatgatc atttagtcaa aaacacagga tgattactgc 70920
tttgactctg ctcttcatca tggtgaccac actcaccttg cctttggtgg ttactgaggg 70980
ctgccgcttg gcttctgcca ccctgggatc caattactac tgttgtattt agatttccca 71040
attcaggggc tgcagttccc attgcaggat ctggtctaca gagaagagtg attgtacagc 71100
tcttcaaatg ttagagctcc tctcaaaaat gtatttctca cagttgtggt gaaagttatg 71160
tcttctggac cctgccaggg tgggtgagta gaccttaaat gacaaattta ctccaaaatt 71220
ccaatctttc tgggcctttg aatccctttc tctacattaa accaaggcaa gtccagcatt 71280
tctaacacac tgtgggccac cttttggtcc atgttttagc caaccacaca aactgttaga 71340
gccctttcta tttccctgag ctgcaacatt aagtgcagaa tctctgctta gtgggctcat 71400
atcaataaat ttagcctcat ccaactttat gttccttcca ccattatacc acacccttaa 71460
tatccattcc cacatatatt ccccagagtc tgtttgtaca aattagaaaa ctcaagtagt 71520
tattttggag tgtagcacac ctcaagggtc acactttgta cctcaccatt agggtcctgc 71580
tgagacttga gtctaggtgt aagtctaaaa ttcaaagggg ttttggggat aagtcctgag 71640
gagaatcggc attgccttgt taggcaatgg actcagagga ggccattacc ttttcctcag 71700
acaatgcagg gttaatccct atagatgagg gtggagagga tgcttctact ggcaaagaag 71760
actcatcaga atttggagga tttttgtccc tcgctccatt aggttcttcc cacatattcc 71820
cattgcaaat tcaggatccc gttctttcct aatcgatacc ttcattctat cagtggacag 71880
aataaccaaa taaacttttt aaaaaagaga tatataaatg gtgttgacaa atatggctta 71940
ccaggtatca aagcataaaa taatgttaca ggaattaaag aagtatgtct caggctgagt 72000
gcggtgattc atgtctgtaa tctcaacact ttaggaggcc aaagcaggag gatcacttga 72060
ggccagatgt ttgagtccag tctggttaac ataaaaagaa catgtctctt aaaaaacaaa 72120
acaaaaaaac caaaaaaaac aaaacaccca aaacaagtat gtctatggct tccagataag 72180
caaatagatg agtggaccaa aatagagatt ccacatcaga ctcatgcata tatgaatgag 72240
taatactttt aatgagtagc aaaatgaaga atcacaaata cttcatcttt acctagaatg 72300
gataacagtg gatattgatt atatatattt accttcatca cctggacatt cctcagaaca 72360
tcagagaata ggagacaaat cctacaaata ttcagcgtcc tatcacatca ataaaatgtt 72420
taggagttta gtggcctgga gcatgccagg acattacttc caaaataaag ggcagattat 72480
tgtactatgt acttcctatc aagaaggaaa acaatacatg ataggctggg aaagtaattt 72540
gcattgtaat tcagtcacag gatacagagc ttgggtatgg tttcagttac gtcagttctg 72600
cagctacagg agataaatgt cttagggcag atatagaagt tttcatgtca tttttatgca 72660
aaaaacttta atgcttgagc tcatcctttt ctttctccat tttgttcagt gacaaggaac 72720
aaccaggcaa tctcattata cttgttagtt tgacaacaat gctcaaaaat attatataca 72780
tggtcactag atgcttgctt cttataagtg tttggttggg agtattcagt ggtaatattt 72840
tgtgtacctc tattattcgt gatagtccag atcacgctag ggactaccaa tgctattttt 72900
actactggaa atagagtcac tcgtatcttt aaaactagcc agattagaga gccaatttca 72960
gaaactctag aaccaattca gaaaattcat cctgcagatt ctgttcctct ggaaccactt 73020
tcagaaccaa aatctgttac tagtcagcat tctgcagaga agctgaatta attatctcta 73080
tctatctatt atctattttc tatctatcta tctatctatc tatctatcta tctatctatc 73140
tatcatctat ctatctatct acctatctaa tctatttacc tatttcctaa aagatttata 73200
tttatcttaa gatattggtt ataaggaatc aactgacgat tttgaagact ggcaagtcca 73260
aaatctgcag agctcatgtc tcagttagaa ggctgtcagg caggagagaa ttctttcttg 73320
gaagaggtca gtctttttct actgaggctg tcagctgatt ggatgaggct cacttacatt 73380
gtggaggatc atctgcttta cttaatctat tgatttaaat atcaatctca tcccaaaaca 73440
tccttatgaa aagactcaga atgctctttg accaaatatc tgtgcatccc atggcccagt 73500
caagttgaca cataaaatga tcatagtgag ctagtcacat ggtaattgct atgtgacacc 73560
atatatgagt tggtgcagta gtggtggatg acaatgacat gcagatctag gctagctgct 73620
catgtgtcct ttttgaactc tctggctttg ttcatgctaa atcccagatg tggcacttgc 73680
acattttaat ggcctgcttc tggttccatc tgaccttatg attttgtgga tctgacagct 73740
catgatcagt agttctggcc acttgtccac ttaatgtccc acgaacaagc tattcatctc 73800
tactagagtt cagtggaaca gcagaatctg tcatttgtaa gctgagtaat tctccactgc 73860
agggagcata atcttgctct ctcatcatta gtgtttgtca taaactttgc acaacatctt 73920
tccccactaa tgatgtctct aatgcaataa tgactactgg ttatataatt taaaaagcag 73980
ggccacttgt accagaatct agtcctattt catagctttt ctctgctcga ggtcctactc 74040
aaagctggaa gcctttcatg tatcctagtg aatgggtaga accaatatct tagatatgca 74100
atacactatc tccagaactt gaacaagtct attaagggtt gtattccttc ttggtgggag 74160
gtgcaaagta caagaatctg tcctctactt cggaggtaac atcatggcat acttcaggcc 74220
actagccccc taaaaaattt attgatgtga caggcccctg aatatttgta ggatttatcc 74280
tctaccctct gttgctctga tggatg 74306

Claims (6)

1. An application of a preparation for detecting the expression quantity of a LINC02085 gene in preparation of a rheumatoid arthritis detection preparation is characterized in that the preparation for detecting the expression quantity of the LINC02085 gene comprises a primer pair with nucleotide sequences shown as SEQ ID No.1 and SEQ ID No. 2.
2. The use of claim 1, wherein said agent for detecting the expression level of LINC02085 gene further comprises: 5uL of 2 XSSYBR Green mix, 1uL of Forward primer (10uM), 1uL of Reverse primer (10uM), 1uL of cDNA at a concentration of 2.5 ng/. mu.l, 1. mu.l each of SEQ ID NO.1 and SEQ ID NO.2 at a concentration of 10. mu.l/L, and 2uL of RNase Free water.
3. A detection method of LINC02085 gene is characterized by comprising the steps of extracting PBMCs from RA peripheral blood mononuclear cells, extracting total RNA by a TRIzol method, measuring RNA concentration and purity, synthesizing cDNA and carrying out PCR amplification.
4. The application of the LINC02085 gene in preparing a rheumatoid arthritis targeted therapy preparation is characterized in that a specific overexpression sequence and a small interference RAN sequence are prepared according to the LINC02085 gene and used for overexpression of the LINC02085 gene in synovial fibroblasts.
5. The use of claim 4, wherein the formulation for targeted therapy of rheumatoid arthritis comprises the specific over-expression sequence pcDNA3.1-LINC02085 and the small interference sequence si-LINC02085, the nucleotide sequence of which is shown in SEQ ID No. 3.
6. The use of claim 5, wherein the specific over-expression sequence pcDNA3.1-LINC02085 and the small interference sequence si-LINC02085 are used to infect synovial fibroblasts, resulting in overexpression and small interference of the LINC02085 gene in the cell.
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CN113278691A (en) * 2021-05-08 2021-08-20 中山大学孙逸仙纪念医院 Long non-coding RNA and application thereof in diagnosis/treatment of rheumatoid arthritis
CN114350785A (en) * 2022-01-05 2022-04-15 安徽中医药大学第一附属医院(安徽省中医院) Application of circular RNA hsa _ circ _0000734 gene

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