CN112342134A - Raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate and using method thereof - Google Patents
Raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate and using method thereof Download PDFInfo
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 62
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 28
- 238000010521 absorption reaction Methods 0.000 title claims abstract description 15
- 229910002651 NO3 Inorganic materials 0.000 title claims abstract description 14
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 title claims abstract description 14
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 14
- 230000001737 promoting effect Effects 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims description 13
- 230000001502 supplementing effect Effects 0.000 claims abstract description 44
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims abstract description 40
- 235000011114 ammonium hydroxide Nutrition 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 241000195493 Cryptophyta Species 0.000 claims abstract description 23
- 238000003756 stirring Methods 0.000 claims abstract description 22
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 5
- 238000002955 isolation Methods 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 39
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 28
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 18
- 239000001569 carbon dioxide Substances 0.000 claims description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 10
- 238000007654 immersion Methods 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 239000002609 medium Substances 0.000 claims description 5
- 239000013589 supplement Substances 0.000 claims description 5
- 239000005708 Sodium hypochlorite Substances 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims description 2
- 238000005192 partition Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000004317 sodium nitrate Substances 0.000 description 3
- 235000010344 sodium nitrate Nutrition 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 150000002823 nitrates Chemical class 0.000 description 2
- 241001536324 Botryococcus Species 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 150000001338 aliphatic hydrocarbons Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 229910001415 sodium ion Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M23/18—Open ponds; Greenhouse type or underground installations
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- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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Abstract
The invention discloses a raceway pond photobioreactor for promoting carbon source absorption by replacing nitrate with ammonia, which comprises a raceway pond body, a separation wall arranged in the raceway pond body, a pH sensor, a stirring paddle for pushing algae liquid to circularly flow along the raceway pond body, a carbon supplementing device for adjusting the pH value of the algae liquid and an ammonia water supplementing device for supplementing ammonia water; the runway pool body is isolated into an annular channel by the isolation wall; the pH sensor is positioned between the carbon supplementing device and the ammonia water supplementing device; the ammonia water replenishing device is positioned at the upstream of the carbon replenishing device.
Description
Technical Field
The invention relates to the field of microalgae culture, in particular to a raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate and a using method thereof.
Background
Microalgae are a class of photoautotrophic microorganisms that can immobilize CO2Producing a variety of chemicals. A one is provided withThe oil-fat-reducing agent can produce aliphatic hydrocarbon, such as the hydrocarbon production amount of the botryococcus can reach 15-75% of the dry weight of cells, some can accumulate glycogen, some can accumulate glycerol, and the oil and fat of a plurality of microalgae can reach more than 60% of the dry weight. And due to the advantages of high growth rate, capability of utilizing waste water and waste gas for culture and the like, the growth promoter has attracted more and more attention worldwide.
At present, the culture method of microalgae is mainly divided into open raceway pond culture and closed photobioreactor culture. Both of them are usually carried out by bubbling a carbon dioxide-containing gas into the culture medium as a carbon source, but this method has a problem that the carbon dioxide absorption rate is low, and therefore the carbon dioxide utilization rate is low and the carbon source cost is high. In addition, a large amount of nitrogen sources are needed in the process of culturing the microalgae, the traditional nitrogen sources are nitrates such as sodium nitrate and the like, however, after the microalgae absorb and metabolize the nitrates, the salinity of the culture solution is increased by sodium ions, and the recycling of the culture medium is adversely affected. Therefore, in order to meet the requirement of rapid growth of microalgae and reduce the culture cost, on one hand, the utilization rate of carbon dioxide needs to be improved, and on the other hand, a nitrogen source needs to be replaced.
Patents CN200510126465.2, CN201210138598.1 and CN201210138845.8 invent the supplement of CO in open pond from the viewpoint of prolonging the gas-liquid contact time and increasing the gas-liquid contact area2The trap carbon supply technology and the horizontal immersion carbon supply technology strengthen the mass transfer process and greatly improve CO2The absorption rate of (c). However, the above patent changes the flow direction of the culture solution in the raceway pond by arranging the carbon supplementing trap in front of or behind the stirring paddle, and the energy consumption of driving is greatly increased in the process that the liquid flows into the trap and then flows out. In addition, the culture solution exchanged with the gas in the horizontal immersion hood carbon supply technology is limited, and carbon dioxide bubbles which are not completely absorbed can be accumulated on the inner wall of the hood and can escape into the air again when the flow rate of the liquid is increased. The inventor carefully researches the consumption rule of the carbon source and the nitrogen source in the culture solution, and finds that the ammonia water instead of the nitrate nitrogen source can fix the carbon dioxide and reduce the cost of water resource circulation treatment without generating toxicity on microalgae cells.
Disclosure of Invention
The invention aims to provide a raceway pond photobioreactor for promoting carbon source absorption by replacing nitrate with ammonia, and aims to solve the problems that in the prior art, the utilization rate of carbon dioxide is low and the water circulation treatment cost of the photobioreactor is high.
In order to achieve the purpose, the invention adopts the following technical scheme: a raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate comprises a raceway pond body, a partition wall arranged in the raceway pond body, a pH sensor, a stirring paddle, a carbon supplementing device for adjusting the pH value of algae liquid and an ammonia water supplementing device for supplementing ammonia water; the stirring paddle is arranged in the raceway pond body and used for pushing the algae liquid to circularly flow along the raceway pond body; the runway pool body is isolated into an annular channel by the isolation wall; the pH sensor is positioned between the carbon supplementing device and the ammonia water supplementing device; the ammonia water replenishing device is positioned at the upstream of the carbon replenishing device.
Further, the ammonia water replenishing device comprises an ammonia water storage tank, a delivery pump and a delivery main pipe arranged in the runway pool body; the ammonia water storage tank and the delivery pump are both arranged outside the raceway pond body; the inlet of the delivery pump is communicated with the ammonia water storage tank through a pipeline, and the outlet of the delivery pump is communicated with the delivery main pipe; the conveying main pipe is immersed in the algae liquid, and a plurality of spray heads are arranged on the conveying main pipe.
Further, the spray head is arranged towards the carbon supplementing device.
Further, the carbon supply device is a trap carbon supply device or a horizontal immersion carbon supply device.
On the other hand, the invention discloses a use method of the raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate,
a. adding a culture medium into the reactor and sterilizing, wherein the depth of the culture medium is 5cm to 30 cm; the culture medium is one of a Zarrouk culture medium, an F/2 culture medium, an SM culture medium, a D1 culture medium and a BG11 culture medium which are not added;
b. starting a stirring paddle to stir the culture medium uniformly, and inoculating microalgae seeds into the culture medium, wherein the inoculation density of the microalgae seeds is 0.01 g/L to 0.5g/L calculated according to a dry medium; adjusting the rotating speed of the stirring paddle to enable the flow speed of the culture medium to be between 10 cm/s and 100 cm/s;
c. starting a delivery pump in the ammonia water supplementing device, supplementing ammonia water into the algae liquid, and maintaining NH in the algae liquid4 +The concentration of (A) is 2mmol/L to 4 mmol/L;
d. according to the pH value of the culture medium detected by the pH sensor, when the pH value is higher than the pH value required by microalgae culture, the carbon supplementing device is started to supplement carbon dioxide into the algae liquid, and when the pH value is lower than the pH value required by microalgae culture, the carbon supplementing device is closed;
e. and detecting the cell concentration of the microalgae, and harvesting the microalgae when the cell concentration of the microalgae is not changed after two consecutive days.
In the above method, the medium used for sterilizing the culture medium is one of ultraviolet rays, sodium hypochlorite and ozone.
The invention has the beneficial effects that: 1. by adopting the reactor provided by the invention and arranging the ammonia water replenishing device, the ammonia water is used for replacing nitrate to be used as a nitrogen source in the culture medium to culture microalgae, the depth of a carbon replenishing trap can be reduced, the utilization rate of a carbon source can be improved, and the energy consumption of the stirring paddle for driving the microalgae liquid to circularly flow can be effectively reduced.
2. The reactor of the invention is used for culturing microalgae, can reduce the salinity of the culture solution, increase the times of the cyclic use of the culture solution and save the cost of water treatment.
3. According to the invention, a direct ammonia water feeding mode is adopted as the nitrogen source for supplementing, the ammonia water supplementing device is arranged at the upstream of the carbon supplementing device, the ammonia water enters the algae liquid and directly reacts with carbon dioxide to be fixed, the operation is convenient, the steps are simple, the escape of ammonia is reduced, and the yield of the nitrogen source is improved.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the structure along the line A-A in example 1 of the present invention;
FIG. 3 is a schematic view of the structure along the line A-A in example 2 of the present invention;
reference numerals: 1-a raceway pond body; 2-a separation wall; 3-pulping; 4-a carbon supplementing device; 41-trap type carbon supply device; 42-horizontal immersion type carbon supplement device; 411-a carbon dioxide aeration device; 5-a pH sensor; 6-conveying a main pipe; 7-a delivery pump; 8-ammonia water storage tank; 9-spray head.
Detailed Description
The technical solution of the present invention will be further described with reference to the accompanying drawings and examples.
Example 1:
a raceway pond photobioreactor for promoting carbon source absorption by replacing nitrate with ammonia comprises a raceway pond body 1, a separation wall 2 arranged in the raceway pond body 1, a pH sensor 5, a stirring paddle 3, a carbon supplementing device 4 for adjusting the pH value of algae liquid and an ammonia water supplementing device for supplementing ammonia water; the circumference of the runway pool body 1 is 70 meters, and the width is 3 meters. The stirring paddle 3 is installed in the raceway pond body 1 and connected with the output end of a motor arranged outside the raceway pond, and is used for pushing algae liquid to flow annularly along the raceway pond body 1. The separation wall 2 is arranged in the center of the raceway pond body 1 and separates the raceway pond body 1 into an annular channel; the pH sensor 5 is positioned between the carbon replenishing device 4 and the ammonia water replenishing device; the ammonia water supplementing device is positioned at the upstream of the carbon supplementing device 4. The ammonia water replenishing device comprises an ammonia water storage tank 8, a delivery pump 7 and a delivery main pipe 6 arranged in the runway pool body 1; the ammonia water storage tank 8 and the delivery pump 7 are both arranged outside the runway pool body 1; an inlet of the delivery pump 7 is communicated with the ammonia water storage tank 8 through a pipeline, and an outlet of the delivery pump is communicated with the delivery main pipe 6; the conveying main pipe 6 is immersed in the algae liquid, and a plurality of spray heads 9 are arranged on the conveying main pipe 6. The spray head 9 is arranged towards the carbon supply device 4. The carbon supply device 4 is a trap carbon supply device 41. The trap carbon supply structure is the structure in embodiment 1 in CN201210138598.1 in the prior art, except that the depth of the carbon supply trap in this embodiment is 30 cm. The detailed structure is not described herein.
Adding a culture medium into the raceway pond body, disinfecting by using sodium hypochlorite, wherein the added culture medium is BG11 culture medium without nitrogen source, and starting a stirring paddle 3Stirring the culture medium uniformly, inoculating microalgae seeds into the culture medium, starting a motor to drive a stirring paddle to stir the culture medium uniformly, and inoculating the microalgae seeds into the culture medium, wherein the microalgae seeds are chlorella and are from a freshwater algae seed bank of aquatic organisms of China academy of sciences, and the number of the microalgae seeds is 29. The inoculation density of the microalgae species is 0.3g/L calculated according to a dry basis; adjusting the rotating speed of a motor of the stirring paddle to enable the flow speed of the culture medium to be between 50cm/s and 60 cm/s; starting a delivery pump in the ammonia water supplementing device, supplementing ammonia water into the algae liquid, and maintaining NH in the algae liquid4 +The concentration of (A) is 4 mmol/L; the pH sensor in this example is a commercially available pH electrode, and the probe extends below the microalgae culture liquid level. Controlling the pH value to be 7.8 according to the pH value of the culture medium detected by the pH sensor, and opening a valve on a carbon dioxide aeration pipe on the carbon supplementing device to supplement carbon dioxide into the algae liquid when the pH value is higher than 7.8; and when the cell concentration of the microalgae is lower than 7.8, closing the carbon supplementing device to detect the cell concentration of the microalgae, and harvesting the microalgae when the cell concentration of the microalgae is not changed after two continuous days.
In this example, the yield of algal cells per unit area calculated after 6 days of culture was 11.5 g/(m)2) D, calculating CO by material balance2The utilization rate of the stirring paddle is 80 percent, and the total power consumption of the stirring paddle is 30 kilowatt-hours.
The comparative example of this example is different from example 1 in that a conventional sodium nitrate was used as a nitrogen source in a medium, the depth of a carbon supply well of a carbon supply device was 60cm, and the other conditions were the same. Comparative example the yield per unit area of algal cells after 6 days of culture was 9.3 g/(m)2) D, calculating to obtain CO2The utilization rate of (A) is 66%, and the total electricity consumption of the stirring paddle is 45 kilowatt-hours.
Example 2:
the difference between this example and the raceway pond photobioreactor in example 1 is that the adopted carbon supplementing structure is a horizontal immersion type carbon supplementing device, and the structure of the horizontal immersion type carbon supplementing device refers to example 1 in CN 201210138845.8. The method of use of this example is the same as example 1.
The productivity of algal cells per unit area calculated after 6 days of culture in this example was 11.8 g/(m)2) D, balancing CO by material2The utilization ratio of (A) was 87%.
The comparative example of example 2 used the same raceway pond photobioreactor as in example 2, and the same culture conditions were used, except that: the traditional sodium nitrate is adopted as a nitrogen source in the culture medium, and after 6 days of culture, the yield of the algae cells per unit area is 9.0 g/(m)2) D, calculating to obtain CO2The utilization ratio of (2) was 60%.
Obviously, the technical scheme of the invention has the advantages of simpler operation, obviously increased utilization rate of carbon dioxide and obviously reduced energy consumption.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (6)
1. A raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate is characterized in that: the device comprises a runway pool body (1), a partition wall (2) arranged in the runway pool body (1), a pH sensor (5), a stirring paddle (3), a carbon supplementing device (4) for adjusting the pH value of algae liquid and an ammonia water supplementing device for supplementing ammonia water; the stirring paddle (3) is arranged in the raceway pond body (1) and is used for pushing the algae liquid to circularly flow along the raceway pond body (1); the runway pool body (1) is isolated into an annular channel by the isolation wall (2); the pH sensor (5) is positioned between the carbon supplementing device (4) and the ammonia water supplementing device; the ammonia water supplementing device is positioned at the upstream of the carbon supplementing device (4).
2. The raceway pond photobioreactor as recited in claim 1, wherein ammonia is used to replace nitrate to promote carbon source absorption, and wherein: the ammonia water replenishing device comprises an ammonia water storage tank (8), a delivery pump (7) and a delivery main pipe (6) arranged in the runway pool body (1); the ammonia water storage tank (8) and the delivery pump (7) are both arranged outside the runway pool body (1); the inlet of the delivery pump (7) is communicated with the ammonia water storage tank (8) through a pipeline, and the outlet of the delivery pump is communicated with the delivery main pipe (6); the conveying main pipe (6) is immersed in the algae liquid, and a plurality of spray heads (9) are arranged on the conveying main pipe (6).
3. A raceway pond photobioreactor according to claim 2, wherein ammonia is used to replace nitrate to promote carbon source absorption: the spray head (9) is arranged towards the carbon supplementing device (4).
4. The raceway pond photobioreactor as recited in claim 1, wherein ammonia is used to replace nitrate to promote carbon source absorption, and wherein: the carbon supply device (4) is a trap type carbon supply device (41) or a horizontal immersion type carbon supply device (42).
5. A method for using a raceway pond photobioreactor according to claim 2, wherein the carbon source absorption is promoted by ammonia instead of nitrate, the method comprising:
a. adding a culture medium into the reactor and sterilizing, wherein the depth of the culture medium is 5cm to 30 cm; the culture medium is one of a Zarrouk culture medium, an F/2 culture medium, an SM culture medium, a D1 culture medium and a BG11 culture medium which are not added with a nitrogen source;
b. starting a stirring paddle (3) to stir the culture medium uniformly, and inoculating microalgae seeds into the culture medium, wherein the inoculation density of the microalgae seeds is 0.01 g/L to 0.5g/L calculated according to a dry medium; adjusting the rotating speed of the stirring paddle (3) to ensure that the flow speed of the culture medium is between 10 cm/s and 100 cm/s;
c. starting a delivery pump (7) in the ammonia water supplementing device, supplementing ammonia water into the algae liquid, and maintaining NH in the algae liquid4 +The concentration of (A) is 2mmol/L to 4 mmol/L;
d. according to the pH value of the culture medium detected by the pH sensor (5), when the pH value is higher than the pH value required by microalgae culture, the carbon supplementing device (4) is started to supplement carbon dioxide into the algae liquid, and when the pH value is lower than the pH value required by microalgae culture, the carbon supplementing device (4) is closed;
e. and detecting the cell concentration of the microalgae, and harvesting the microalgae when the cell concentration of the microalgae is not changed after two consecutive days.
6. The method for using a raceway pond photobioreactor as recited in claim 5, wherein the raceway pond photobioreactor comprises: the disinfection medium used when the culture medium is disinfected is one of ultraviolet rays, sodium hypochlorite and ozone.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114032176A (en) * | 2021-11-26 | 2022-02-11 | 海南绿藻世界生物科技有限公司 | Culture system and culture method for promoting growth of microalgae |
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| CN101659922A (en) * | 2008-08-28 | 2010-03-03 | 青岛生物能源与过程研究所 | Closed raceway pond microalgae culture system |
| WO2015110031A1 (en) * | 2014-01-26 | 2015-07-30 | 武汉凯迪工程技术研究总院有限公司 | Method and device for high-density continuous culture of microalgae |
| CN105483013A (en) * | 2015-12-25 | 2016-04-13 | 中国科学院武汉植物园 | Method and device for synchronously producing oil, sequestrating carbon, desulfurizing and denitrifying by utilizing microalgaes |
| CN214193258U (en) * | 2020-12-15 | 2021-09-14 | 信阳碧园生物科技有限公司 | Raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate |
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| CN101659922A (en) * | 2008-08-28 | 2010-03-03 | 青岛生物能源与过程研究所 | Closed raceway pond microalgae culture system |
| WO2015110031A1 (en) * | 2014-01-26 | 2015-07-30 | 武汉凯迪工程技术研究总院有限公司 | Method and device for high-density continuous culture of microalgae |
| CN105483013A (en) * | 2015-12-25 | 2016-04-13 | 中国科学院武汉植物园 | Method and device for synchronously producing oil, sequestrating carbon, desulfurizing and denitrifying by utilizing microalgaes |
| CN214193258U (en) * | 2020-12-15 | 2021-09-14 | 信阳碧园生物科技有限公司 | Raceway pond photobioreactor for promoting carbon source absorption by ammonia instead of nitrate |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114032176A (en) * | 2021-11-26 | 2022-02-11 | 海南绿藻世界生物科技有限公司 | Culture system and culture method for promoting growth of microalgae |
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