CN112322499A - Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage - Google Patents
Autotrophic heterotrophic alternative domestication cultured dunaliella salina and application thereof in treatment of marine ship domestic sewage Download PDFInfo
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- 239000010865 sewage Substances 0.000 title claims abstract description 54
- 241000195633 Dunaliella salina Species 0.000 title claims abstract description 50
- 230000001651 autotrophic effect Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 20
- 239000013535 sea water Substances 0.000 claims abstract description 10
- 241000195493 Cryptophyta Species 0.000 claims abstract description 9
- 238000005286 illumination Methods 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 230000001276 controlling effect Effects 0.000 claims description 3
- 238000005553 drilling Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims 1
- 239000013505 freshwater Substances 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 239000010802 sludge Substances 0.000 description 7
- 239000002351 wastewater Substances 0.000 description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- 229910052698 phosphorus Inorganic materials 0.000 description 5
- 239000011574 phosphorus Substances 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000003851 biochemical process Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000010842 industrial wastewater Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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Abstract
The invention discloses a Dunaliella salina cultured by autotrophic and heterotrophic alternative domestication and application thereof in treatment of marine ship domestic sewage, belonging to the technical field of biology of algae plants for treating pollutants in the environment. The invention relates to a Dunaliella salina which is purchased from an algae culture mechanism or is domesticated and cultured by a culture solution prepared by mixing domestic sewage of an ocean working platform and seawater, the illumination time is controlled in the culture process to realize the autotrophic and heterotrophic process of the Dunaliella salina, and after the domestication and culture are finished, the domesticated Dunaliella salina is expanded and cultured to be used for treating the domestic sewage of the ocean working platform. The Dunaliella salina cultured by autotrophic heterotrophic alternative domestication is from the sea, is beneficial to reducing the consumption of fresh water when culturing and treating sewage, can save the step of terminal disinfection treatment, and can not cause secondary pollution when being directly discharged into the sea.
Description
Technical Field
The invention relates to the field of biotechnology of treating pollutants in environment by using algae plants, in particular to a method for treating ship domestic sewage by using Dunaliella salina.
Background
In recent years, environmental protection has become a hot topic. Since the 20 th century, industrial wastewater and domestic sewage are produced in large quantities, fresh water resources on the earth are not abundant, so that the wastewater needs to be treated in a scientific, advanced, green and low-energy-consumption mode and can be discharged up to the standard or subjected to advanced treatment and recycling.
Offshore ships, drilling work platforms, large-scale mail ships and warships usually generate a large amount of domestic sewage, and the main treatment process still uses the traditional activated sludge process, so that the consumption of fresh water is greatly increased. Seawater can hardly be used for washing and diluting a biochemical pool, and the seawater salinity is high, so that the conductivity is often about 10000 mus/cm, and the growth of activated sludge strains is seriously inhibited. Thereby greatly reducing the processing efficiency. Meanwhile, the treated sludge is usually required to be disinfected and then can be discharged into the sea, so that the dosage of the medicament is greatly increased.
The traditional domestic sewage treatment system of ships has small space and large condition limitation, so that the original activity may be lost after the inoculation of the activated sludge, the sludge is dead or the activity is reduced, and the sludge in biochemical effluent is discharged into the sea. Since the ship floats on the sea all the year round, the microbial culture is troublesome, and fresh water resources are wasted, the research and development of a scientific, advanced, green and low-energy-consumption water treatment method is urgent.
Disclosure of Invention
The invention aims to provide a Dunaliella salina for treating ship domestic sewage, which treats biochemical end wastewater in ship factory wastewater by using marine green microalgae (Dunaliella salina). since the Dunaliella salina is from the sea, the Dunaliella salina is not only beneficial to reducing the consumption of fresh water, but also can save the end disinfection treatment step, and can not cause secondary pollution when being directly discharged into the sea.
The invention provides an autotrophic and heterotrophic alternative domestication and culture dunaliella salina and application thereof in treatment of marine vessel domestic sewage, which is characterized by comprising the following steps:
(a1) algae screening: inoculating Dunaliella salina on BG11 solid culture medium, adding artificial seawater, setting culture temperature at 25 deg.C, and regulating initial pH to 6.8;
(a2) preparing a domestication culture solution: preparing a targeted domestication culture solution from marine ship domestic sewage and seawater according to a volume ratio of 1:1, regulating and controlling the pH of the culture solution to be 6.8, wherein the concentration of the domestic sewage is gradually increased and is respectively 25%, 50%, 75% and 100%;
(a3) domestication and culture: transplanting the Dunaliella salina screened in the step (a1) into a prepared domestication culture solution, gradually increasing the concentration of biological sewage in the domestication culture solution, setting the temperature of a constant temperature shaking table to be 25 ℃, the rotating speed to be 100 plus of 200rpm/min, keeping the illumination time for 12h in the domestication culture process, and simulating day and night alternation to realize the autotrophic and heterotrophic processes of the Dunaliella salina;
preferably, the Dunaliella salina is purchased from an algae cultivation institution.
Preferably, the domesticated Dunaliella salina is first subjected to expanded culture, the domesticated Dunaliella salina is transplanted into a 50-100LBG11 culture medium, marine domestic sewage with 100% concentration is added, the culture amount of the Dunaliella salina is increased, and the Dunaliella salina subjected to expanded culture is used for treating the marine biological sewage.
Preferably, the sewage is domestic sewage generated by large offshore working platforms such as offshore ships, drilling working platforms, large mail ships and warships.
Preferably, the temperature of the sewage is controlled to be 25 ℃ during the sewage treatment, and the pH of the sewage is controlled to be 6.0-7.0.
The invention provides a Dunaliella salina cultured by autotrophic and heterotrophic alternative domestication and application thereof in treatment of marine vessel domestic sewage, which has the beneficial effects that:
(1) the Dunaliella salina is used for treating ship domestic sewage, has an ideal effect of removing pollutants from the domestic sewage, and can be directly discharged into the sea without disinfection.
(2) The Dunaliella salina is used for treating ship domestic sewage, greatly improves the ship closed operation environment, does not generate odor and sludge of the traditional biochemical process, and is green and environment-friendly.
(3) The Dunaliella salina is used for treating ship domestic sewage, has low operation cost, and can reduce the treatment cost of power consumption, drug consumption and the like.
(4) The Dunaliella salina is used for treating ship domestic sewage, can be suitable for treating sewage with high salinity, can be continuously used for treating the domestic sewage as long as an original algae sample is stored on a ship when water enters a cabin or an accident happens, has strong recoverability and is suitable for a living environment similar to the ocean.
Drawings
FIG. 1 is a schematic diagram of a Dunaliella salina acclimatization and culture technique for autotrophic and heterotrophic alternative acclimatization and culture according to the present invention;
FIG. 2 is a schematic diagram of an experimental process for treating Dunaliella salina sewage by autotrophic and heterotrophic alternative domestication culture.
Detailed Description
The invention is further illustrated by the following examples.
Example 1
An autotrophic and heterotrophic alternative domestication cultured Dunaliella salina and application thereof in marine vessel biological wastewater treatment comprise three steps of algae screening, domestication culture solution preparation and autotrophic and heterotrophic alternative domestication culture, as shown in figure 1. Inoculating purchased Dunaliella salina into BG11 solid culture medium, adding artificial seawater, regulating and controlling the pH of the culture solution to 6.8, and setting the culture temperature to 25 ℃; transplanting the Dunaliella salina screened from the Dunaliella salina into a targeted domestication culture solution prepared by marine ship domestic sewage and seawater according to a volume ratio of 1:1, wherein the initial domestic sewage concentration is 25%, the pH of the culture solution is kept at 6.8 in the domestication culture process, the temperature of a constant temperature shaking table is 25 ℃, the rotating speed is set to be 100rpm/min, and the illumination time is 12 h; increasing the concentration of the domestic sewage to 50 percent, mixing the domestic sewage with seawater according to the volume ratio of 1:1, transplanting the Dunaliella salina domesticated by the domestic sewage with the concentration of 25 percent into a culture solution, further increasing the concentration of the domestic sewage to 75 percent after the culture is finished, and finishing the domestication culture by 100 percent.
Inoculating the successfully domesticated Dunaliella salina into a 50LBG11 culture medium, adding 100% concentration marine domestic sewage, increasing the culture amount of fresh water Dunaliella salina, and applying the Dunaliella salina obtained by enlarged culture into the marine domestic sewage with the temperature of 25 ℃ and the pH value of 6.0-7.0. The principle of removing organic carbon, nitrogen and phosphorus in marine ship domestic sewage by Dunaliella salina is shown in figure 2, the Dunaliella salina performs photosynthesis to realize autotrophy when being illuminated, absorbs nitrogen and phosphorus in the domestic sewage and absorbs the total nitrogen and phosphorus in the sewage; oxygen generated in the autotrophic process also enters the heterotrophic process, so that the addition of the oxygen in the heterotrophic process is reduced, and the running cost is reduced; in the absence of illumination, the heterotrophic process of Dunaliella salina further consumes total nitrogen and total phosphorus in the wastewater and absorbs organic Carbon (COD) in the wastewater, thereby achieving the effect of removing the organic carbon, nitrogen and phosphorus in the wastewater simultaneously.
Example 2
This example is essentially identical to the embodiment of example 1, with the only difference that the rotational speed of the shaker during acclimatization culture is set at 150rpm/min, and that the medium content during further scale-up culture is 75L.
Example 3
This example is substantially identical to the embodiment of example 1, except that the rotational speed of the shaker during acclimatization culture is set to 200rpm/min, and the content of the medium during further scale-up culture is 100L.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (5)
1. An autotrophic heterotrophic alternative domestication cultured dunaliella salina and an application thereof in treatment of marine vessel domestic sewage are characterized by comprising the following steps:
(a1) algae screening: inoculating Dunaliella salina on BG11 solid culture medium, adding artificial seawater, setting culture temperature at 25 deg.C, and regulating initial pH to 6.8;
(a2) preparing a domestication culture solution: preparing a targeted domestication culture solution from marine ship domestic sewage and seawater according to a volume ratio of 1:1, regulating and controlling the pH of the culture solution to be 6.8, wherein the concentration of the domestic sewage is gradually increased and is respectively 25%, 50%, 75% and 100%;
(a3) domestication and culture: transplanting the Dunaliella salina screened in the step (a1) into a prepared domestication culture solution, gradually increasing the concentration of biological sewage in the domestication culture solution, setting the temperature of a constant temperature shaking table to be 25 ℃, the rotating speed to be 100 plus materials and 200rpm/min, keeping the illumination time for 12h, simulating day and night alternation, and realizing the autotrophic and heterotrophic processes of the Dunaliella salina.
2. The method as claimed in claim 1, wherein the Dunaliella salina is obtained from algae cultivation mechanism.
3. The method as claimed in claim 1-2, wherein the domesticated dunaliella salina is first expanded and cultured, the domesticated dunaliella salina is transplanted into 50-100L BG11 culture medium, 100% concentration marine sewage is added to increase the culture amount of the dunaliella salina, and the expanded and cultured dunaliella salina is used for treating marine biological sewage.
4. The method for treating domestic sewage of marine vessels by using the dunaliella salina cultured by autotrophic and heterotrophic alternative domestication according to the claims 1-3, wherein the sewage is the domestic sewage produced by marine vessels, drilling work platforms, large mail ships, warships and other marine large work platforms.
5. The method for treating domestic sewage of marine vessels by using the dunaliella salina cultured by autotrophic and heterotrophic alternative domestication according to the claims 1-4, wherein the temperature of the sewage is controlled at 25 ℃, and the pH of the sewage is controlled at 6.0-7.0.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114394677A (en) * | 2022-01-18 | 2022-04-26 | 山东海之宝海洋科技有限公司 | Method for treating salt-containing wastewater |
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2020
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Application publication date: 20210205 |