CN112322448A - Haematococcus pluvialis photobioreactor coupled with nutrient salt recovery module - Google Patents
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- CN112322448A CN112322448A CN202011164331.0A CN202011164331A CN112322448A CN 112322448 A CN112322448 A CN 112322448A CN 202011164331 A CN202011164331 A CN 202011164331A CN 112322448 A CN112322448 A CN 112322448A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The utility model provides a haematococcus pluvialis photobioreactor of module is retrieved to coupling nutritive salt, the reactor comprises three cuboid cavity (3,5,6) from the left hand right side and sets up in the outside nutritive salt recovery module of reactor, and middle cavity (5) are used for the culture of the green cell that moves about of haematococcus pluvialis, and cavity (3,6) of the left and right sides are used for the culture of the red pachysolen pluvialis red pachysospore. The invention provides a haematococcus pluvialis photobioreactor coupled with a nutrient salt recovery module by regulating and controlling the illumination intensity of haematococcus pluvialis in different growth stages and the concentration of inorganic nutrient salts such as nitrogen, phosphorus and the like according to the corresponding relation between the biochemical conversion process of the haematococcus pluvialis and the environment working conditions, and realizes synchronous and efficient implementation of biomass accumulation in a green stage of the haematococcus pluvialis and astaxanthin synthesis in cells in a red stage.
Description
Technical Field
The invention relates to a photobioreactor for efficiently culturing microalgae, in particular to a haematococcus pluvialis photobioreactor coupled with a nutrient salt recovery module.
Background
Astaxanthin is a high-value bioactive substance in the current international research and development field, has physiological functions of preventing cardiovascular and cerebrovascular diseases, inhibiting tumors, enhancing immunity and the like, is widely applied to industries of foods, health products, medicines and the like, and has wide application prospect and huge market potential. Haematococcus pluvialis is recognized as the currently most commercially valuable source of natural astaxanthin due to its ability to synthesize astaxanthin in large quantities under environmental stress conditions.
The accumulation of biomass of haematococcus pluvialis and the synthesis of astaxanthin are realized through photosynthesis, and the concentrations of inorganic nutrient salts such as light energy, nitrogen, phosphorus and the like are two main environmental factors influencing the photosynthetic biochemical transformation process of haematococcus pluvialis. It is worth noting that haematococcus pluvialis has growth characteristics remarkably different from most algae, specifically, when the illumination intensity is low and inorganic nutrient salts such as nitrogen and phosphorus are sufficient, haematococcus pluvialis rapidly divides and proliferates in a green swimming cell form, the cell number is increased, and the biomass concentration is increased. However, when the light intensity is high and the concentration of inorganic nutrient salts such as nitrogen and phosphorus is low or completely exhausted, haematococcus pluvialis cells stop dividing and proliferating, green motile cells are gradually changed into red chlamydospore cells, and the content of astaxanthin in the cells is increased. It is thus understood that in order to obtain a high astaxanthin production, it is necessary to ensure both that as high a biomass concentration as possible of Haematococcus pluvialis is obtained and that the astaxanthin content in the cells of Haematococcus pluvialis is as high as possible. In order to achieve the aim, the haematococcus pluvialis green swimming cells in the green stage are placed in an environment with low light intensity and sufficient inorganic nutrient salts such as nitrogen and phosphorus, and the haematococcus pluvialis red chlamydospores in the red stage are placed in an environment with high light intensity and insufficient inorganic nutrient salts such as nitrogen and phosphorus, so that the synthesis of astaxanthin in the haematococcus pluvialis is induced.
Disclosure of Invention
Based on the problems, in order to realize synchronous and efficient implementation of biomass accumulation in the green stage of haematococcus pluvialis and astaxanthin synthesis in cells in the red stage, the photobiological reactor for haematococcus pluvialis is provided with a coupling nutrient salt recovery module by regulating and controlling the illumination intensity of different growth stages of haematococcus pluvialis and the concentration of inorganic nutrient salts such as nitrogen, phosphorus and the like according to the corresponding relation between the biochemical conversion process of haematococcus pluvialis and the environmental working condition.
The technical scheme adopted by the invention is as follows: the utility model provides a haematococcus pluvialis photobioreactor of module is retrieved to coupling nutritive salt, the reactor comprises three cuboid cavity (3,5,6) from the left hand right side and sets up in the outside nutritive salt recovery module of reactor, and middle cavity (5) are used for the culture of the green cell that moves about of haematococcus pluvialis, and cavity (3,6) of the left and right sides are used for the culture of the red pachysolen pluvialis red pachysospore.
Furthermore, the chambers (3,5,6) are made of transparent materials, and the light sources are arranged on the left side of the chamber (3) and the right side of the chamber (6).
Furthermore, the nutrient salt recovery module is connected with the reactor chamber through a hose, a microporous filter tube bundle is arranged in the nutrient salt recovery module, liquid flowing in the microporous filter tube is deionized water, and green swimming cell suspension of haematococcus pluvialis flows outside the tube.
Furthermore, deionized water containing inorganic nutrient salts and flowing out of the nutrient salt recovery module enters the middle cavity of the reactor, and the haematococcus pluvialis green swimming cell suspension in the middle cavity of the reactor is diluted, so that the penetration capacity of light is enhanced, and the utilization efficiency of the nutrient salts is improved.
Furthermore, the transparent material is glass or organic glass.
Furthermore, the nutrient salt recovery module is of a cylindrical structure.
Further, the average pore diameter of the wall surface of the microporous filter tube is 0.45, 0.8 or 1.2 μm.
The utility model provides a haematococcus pluvialis photobioreactor of coupling nutritive salt recovery module, this reactor comprises three cuboid cavitys from left to right, and wherein middle cavity is the culture cavity of the green swimming cell of haematococcus pluvialis, and two left and right cavities are the culture cavity of haematococcus pluvialis red pachydig spore. The reactor is made of transparent materials such as organic glass plates, quartz plates and the like. The light emitted by the fluorescent lamp tube, the LED lamp panel and the like is incident from the left side and the right side of the reactor, the high-intensity incident light entering the reactor firstly enters the red chlamydospore chambers of haematococcus pluvialis at the left side and the right side of the reactor, and the light intensity is reduced along the light transmission direction due to the absorption and scattering effect of the algae cells on the light energy. Then, low-intensity light energy enters a haematococcus pluvialis green swimming cell culture chamber in the middle of the reactor to provide light energy for division and proliferation of haematococcus pluvialis green swimming cells.
The nutrient salt recovery module is arranged outside the reactor, the module is of a cylindrical structure, the microporous filter tube bundle is arranged inside the reactor, the average pore diameter of the wall surface of the microporous filter tube is smaller than the size of haematococcus pluvialis cells, the average pore diameter of the wall surface of a common filter tube is 0.45, 0.8 and 1.2 mu m, liquid flowing in the tube is deionized water, and green haematococcus pluvialis swimming cell suspension flows outside the tube. The nutrient salt recovery module is connected with the reactor through hoses such as a silicone tube, a PU tube and the like.
In the actual working process, when the concentration of the haematococcus pluvialis green swimming cell suspension in the intermediate chamber of the reactor reaches a certain value, the haematococcus pluvialis cell suspension in the intermediate chamber enters the nutrient salt recovery module under the driving of a peristaltic pump and the like and flows outside the pipe of the microporous filter pipe. At the moment, deionized water without nutrient salt enters the nutrient salt recovery module and flows in the microporous filter tube, and because a large amount of inorganic nutrient salts such as nitrogen and phosphorus are contained in the haematococcus pluvialis green swimming cell suspension, the inorganic nutrient salts such as nitrogen and phosphorus in the haematococcus pluvialis suspension outside the tube are diffused into the deionized water in the microporous filter tube through micropores under the driving of the concentration gradient of the inorganic nutrient salts such as nitrogen and phosphorus inside and outside the microporous filter tube, the concentration of the inorganic nutrient salts such as nitrogen and phosphorus in the haematococcus pluvialis suspension is reduced, and meanwhile, algae cells in the haematococcus pluvialis suspension cannot enter the deionized water in the microporous filter tube because the diameter of the algae cells is larger than the wall aperture of the microporous filter tube.
And then, the concentration of inorganic nutrient salts such as nitrogen, phosphorus and the like in the deionized water in the microporous filter tube is increased, and finally the inorganic nutrient salts flow out of the nutrient salt recovery module to enter a middle cavity of the reactor and are assimilated and utilized by haematococcus pluvialis green swimming cells in the middle cavity. The concentration of inorganic nutrient salts such as nitrogen and phosphorus in the haematococcus pluvialis green swimming cell suspension in the nutrient salt recovery module is reduced, and the inorganic nutrient salts flow out of the nutrient salt recovery module and then respectively enter the left cavity and the right cavity of the reactor. In the left and right chambers, under the synergistic stress of inorganic nutritive salts such as high light intensity and low-concentration nitrogen and phosphorus, haematococcus pluvialis is induced to be converted into red chlamydospores from green zooblast, and a large amount of astaxanthin is synthesized in cells.
The invention has the beneficial effects that: through setting up nutritive salt recovery module and different reaction chambers, realize the regulation and control of the illumination intensity of different growth stages of haematococcus pluvialis and the concentration of inorganic nutritive salts such as nitrogen phosphorus to the synchronous high-efficient of haematococcus pluvialis green stage biomass accumulation and red stage intracellular astaxanthin synthesis has been realized.
Drawings
FIG. 1 is a schematic diagram of a three-dimensional structure of a Haematococcus pluvialis photobioreactor coupled with a nutrient salt recovery module according to the present invention;
FIG. 2 is a schematic diagram of the operating principle of a Haematococcus pluvialis photobioreactor coupled with a nutrient salt recovery module according to the present invention; wherein, in the drawings: 1-incident light; 2-haematococcus pluvialis red chlamydospore suspension in a cavity at the left side of the reactor; 3-left chamber of reactor; 4-haematococcus pluvialis green swimming cell suspension in the middle chamber of the reactor; 5-reactor middle chamber; 6-right chamber of reactor; 7-haematococcus pluvialis red chlamydospore suspension in a cavity at the right side of the reactor; 8-reactor gas outlet; 9-nutrient salt recovery module; 10-direction of flow of haematococcus pluvialis cell suspension; 11-haematococcus pluvialis green swimming cell suspension containing low-concentration inorganic nutrient salts such as nitrogen and phosphorus; 12-a microporous filter tube; 13-deionized water containing inorganic nutrient salts such as nitrogen and phosphorus; 14-deionized water flow direction; 15-haematococcus pluvialis green swimming cell suspension containing inorganic nutritive salts such as sufficient nitrogen and phosphorus; 16-deionized water without inorganic nutrient salts such as nitrogen, phosphorus and the like.
Detailed Description
The invention is further illustrated by the following examples in conjunction with the accompanying drawings:
referring to fig. 1 and 2, the haematococcus pluvialis photobioreactor of a coupling nutrient salt recovery module is formed by processing transparent materials such as organic glass, glass and quartz, and the reactor main body comprises three cuboid rectangular chambers, namely a reactor left chamber 3, a reactor middle chamber 5 and a reactor right chamber 6. The middle chamber 5 of the reactor is filled with a haematococcus pluvialis green zooblast suspension 4 in a green growth stage, and the left chamber 3 and the right chamber 6 of the reactor are filled with haematococcus pluvialis red chlamydospore suspensions 2 and 7 in a red stage.
In this embodiment, the light emitted from the LED lamp panels on the left and right sides of the reactor provides incident light 1 for the reactor, after the incident light 1 enters the reactor, the incident light first enters the chambers on the left and right sides of the reactor, that is, the left chamber 3 and the right chamber 6 of the reactor, and then is absorbed or scattered by the haematococcus pluvialis red chlamydospore suspensions 2 and 7 in the chambers on the left and right sides of the reactor, and along the incident light direction, the light intensity is exponentially attenuated, and then, the light with lower intensity enters the middle chamber 5 of the reactor, so as to provide light energy for the haematococcus pluvialis cells in the haematococcus pluvialis green swimming cell suspension 4 in the middle chamber 5 of the reactor. Under the conditions of low illumination and sufficient content of inorganic nutrient salts such as nitrogen and phosphorus, the haematococcus pluvialis green swimming cells in the haematococcus pluvialis green swimming cell suspension 4 in the middle chamber 5 of the reactor are rapidly divided and proliferated, and the concentration of the haematococcus pluvialis green swimming cell suspension 4 is increased.
In this embodiment, the biomass concentration of the Haematococcus pluvialis green motile cell suspension 4 in the intermediate chamber 5 of the reactor reaches 1.2g L-1In the meantime, 50% by volume of haematococcus pluvialis green motile cell suspension 15 (containing sufficient inorganic nutrient salts such as nitrogen and phosphorus) in the intermediate chamber 5 of the reactor enters the nutrient salt recovery module 9 under the driving of the peristaltic pump. The nutritive salt recovery module 9 is cylindrical in shape, and is internally densely provided with a microporous filter pipe 12 pipe bundle, the inner diameter of the microporous filter pipe is 2mm, and the average pore diameter of the wall surface is 0.45 mu m. In the nutrient salt recovery module 9, haematococcus pluvialis green swimming cell suspension containing inorganic nutrient salts such as sufficient nitrogen and phosphorus flows outside the microporous filter pipe 12. Meanwhile, deionized water 16 without inorganic nutrient salts such as nitrogen and phosphorus enters the pipe of the microporous filter pipe 12 in the nutrient salt recovery module 9 under the drive of the peristaltic pump, and under the drive of concentration gradient, inorganic nutrient salts such as nitrogen and phosphorus in the haematococcus pluvialis green swimming cell suspensionNutrient salts enter deionized water in the microporous filter pipe through micropores in the pipe wall of the microporous filter pipe 12, and then deionized water 13 containing inorganic nutrient salts such as nitrogen and phosphorus finally enters a reactor middle chamber 5, on one hand, inorganic nutrient salts such as nitrogen and phosphorus are provided for haematococcus pluvialis green swimming cell suspension 4 in the reactor middle chamber 5, on the other hand, the haematococcus pluvialis green swimming cell suspension 4 in the reactor middle chamber 5 is diluted, and the penetration capacity of light on the haematococcus pluvialis green swimming cell suspension 4 in the reactor middle chamber 5 is enhanced.
In this embodiment, through the exchange of inorganic nutrient salts such as nitrogen and phosphorus in the nutrient salt recovery module 9, the haematococcus pluvialis green swimming cell suspension 11 containing inorganic nutrient salts such as low-concentration nitrogen and phosphorus respectively enters the left chamber 3 and the right chamber 6 of the reactor, and in the chambers on the left and right sides of the reactor, under the synergistic stress of high illumination intensity and low inorganic salt concentration such as nitrogen and phosphorus, the haematococcus pluvialis green swimming cells are induced to be converted into haematococcus pluvialis red chlamydospores, and a large amount of astaxanthin is synthesized in the haematococcus pluvialis red chlamydospores. Therefore, the haematococcus pluvialis photobioreactor coupled with the nutrient salt recovery module can realize the synchronous implementation of biomass accumulation in the green stage of the haematococcus pluvialis and the induced synthesis of astaxanthin in the red stage of the haematococcus pluvialis, on one hand, the efficient utilization of light energy is realized, and on the other hand, the regulation and control of inorganic nutrient salts such as nitrogen and phosphorus in the haematococcus pluvialis cell suspension are realized through the introduction of the nutrient salt recovery module 9.
Claims (7)
1. The utility model provides a haematococcus pluvialis photobioreactor that module was retrieved to coupling nutritive salt, its characterized in that, the reactor comprises three cuboid cavity (3,5,6) from left right side and the nutritive salt who sets up in the reactor outside retrieves the module, and middle cavity (5) are used for the culture of the green cell that moves about of haematococcus pluvialis, and cavity (3,6) of the left and right sides are used for the culture of the red pachyron spore of haematococcus pluvialis.
2. The photobioreactor as claimed in claim 1, wherein the chamber (3,5,6) is made of transparent material, and the light source is disposed on the left side of the chamber (3) and the right side of the chamber (6).
3. The photobioreactor as claimed in claim 1, wherein the nutrient salt recovery module is connected to the reactor chamber through a hose, the nutrient salt recovery module is internally provided with a microporous filter tube bundle, the liquid flowing in the microporous filter tube is deionized water, and the liquid flowing outside the microporous filter tube is a haematococcus pluvialis green swimming cell suspension.
4. The Haematococcus pluvialis photobioreactor coupled with the nutrient salt recovery module as claimed in any one of claims 1 to 3, wherein deionized water containing inorganic nutrient salts flowing out of the nutrient salt recovery module enters the middle chamber of the reactor, and the suspension of green swimming cells of Haematococcus pluvialis in the middle chamber of the reactor is diluted, so that the light penetration capacity is enhanced, and the utilization efficiency of the nutrient salts is improved.
5. The photobioreactor as recited in claim 2, wherein the transparent material is glass or plexiglass.
6. The Haematococcus pluvialis photobioreactor coupled to the nutrient salt recovery module of claim 3, wherein the nutrient salt recovery module is of a cylindrical structure.
7. The photobioreactor as recited in claim 3, wherein the mean pore diameter of the wall surface of the microporous filter tube is 0.45, 0.8 or 1.2 μm.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10222214A1 (en) * | 2002-05-16 | 2003-12-18 | Forschungszentrum Juelich Gmbh | Laboratory culture reactor for phototrophic organisms such as algae is fabricated from translucent material and sub-divided into two or more compartments |
| CN202519240U (en) * | 2012-03-01 | 2012-11-07 | 武汉凯迪工程技术研究总院有限公司 | Production device for controlling chytrid fungus pollution in haematococcus pluvialis |
| CN102827766A (en) * | 2012-08-17 | 2012-12-19 | 宁波红龙生物科技有限公司 | Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis |
| CN208440632U (en) * | 2018-06-05 | 2019-01-29 | 河南师范大学 | A kind of simple controllable closed haematococcus pluvialis bioreactor |
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- 2020-10-27 CN CN202011164331.0A patent/CN112322448A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10222214A1 (en) * | 2002-05-16 | 2003-12-18 | Forschungszentrum Juelich Gmbh | Laboratory culture reactor for phototrophic organisms such as algae is fabricated from translucent material and sub-divided into two or more compartments |
| CN202519240U (en) * | 2012-03-01 | 2012-11-07 | 武汉凯迪工程技术研究总院有限公司 | Production device for controlling chytrid fungus pollution in haematococcus pluvialis |
| CN102827766A (en) * | 2012-08-17 | 2012-12-19 | 宁波红龙生物科技有限公司 | Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis |
| CN208440632U (en) * | 2018-06-05 | 2019-01-29 | 河南师范大学 | A kind of simple controllable closed haematococcus pluvialis bioreactor |
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