CN112304929B - Method and device for detecting biogenic amine in food material - Google Patents
Method and device for detecting biogenic amine in food material Download PDFInfo
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- CN112304929B CN112304929B CN201910672704.6A CN201910672704A CN112304929B CN 112304929 B CN112304929 B CN 112304929B CN 201910672704 A CN201910672704 A CN 201910672704A CN 112304929 B CN112304929 B CN 112304929B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
A method for detecting biogenic amines in food materials comprising: providing a biogenic amine detecting element, wherein the biogenic amine detecting element comprises a biogenic amine gas adsorption substrate, and the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporization gas or biogenic amine vapor; disposing a biogenic amine sensitive material on the biogenic amine gas adsorption substrate; combining the biogenic amine detection element with a to-be-detected food material package or a to-be-detected food material; and generating a biogenic amine index by utilizing the biogenic amine sensitive material once the biogenic amine detection element contacts a food material biogenic amine vaporization gas or biogenic amine vapor.
Description
Technical Field
The invention relates to a method for detecting biogenic amine (biogenic amine) of food materials and a device thereof; in particular to a non-contact method and a device for detecting the vaporization gas (moisture or vapor) of the biogenic amine of the food material; more particularly, it relates to a vaporization method and device for detecting biogenic amine in food materials by using biogenic amine sensitive materials.
Background
For example, a technique for detecting biogenic amine is commonly used, such as the patent application of "a method for rapidly detecting biogenic amine" of Chinese patent publication No. CN-102650628, which discloses a method for rapidly detecting biogenic amine. The method for rapidly detecting biogenic amine comprises the following steps: after a biogenic amine standard substance and a sample to be detected are derivatized by a derivatization agent, a coloring group is carried on the sample, the sample is spotted on a chromatographic material polyamide film, then the sample is unfolded by a developing agent, when the front edge of the developing agent is 0.5 to 1cm away from the top end of the film, the chromatography is stopped, the color development is generated, and the color development result is observed.
In view of the above, the aforementioned CN-102650628 employs a simple and rapid analysis method, which uses Thin Layer Chromatography (TLC) for measurement, and has advantages of no need of using expensive analytical equipment, reduced detection cost and rapid detection when compared with other analysis methods, but has disadvantages that biogenic amine contained in food still needs to be extracted and derivatized for detection and determination.
Another common technique for detecting biogenic amines is the invention patent application of "a method for rapidly detecting biogenic amines in foods" in Chinese patent publication No. CN-103149317, which discloses a method for rapidly detecting biogenic amines in foods. The method for rapidly determining biogenic amine in food comprises the following steps: 1. extracting biogenic amine from a food sample; 2. purifying a biogenic amine extract; 3. derivatizing the biogenic amine extract; and 4, optimizing and performing thin layer chromatography analysis by using a developing agent.
In addition, the aforementioned CN-103149317 is also a simple and rapid analysis method, which is also determined by Thin Layer Chromatography (TLC), and has the advantages of no need of expensive analysis equipment, low detection cost and rapid detection when compared with other analysis methods, but has the disadvantage that biogenic amine contained in food still needs to be extracted and derivatized for detection and judgment.
Another commonly used technique for detecting biogenic amines, such as the "method for detecting histamine using an immunosensor" patent application of Chinese patent publication No. CN-104897897, discloses a method for rapidly detecting biogenic amines in food comprising the steps of: s1, preparing an immune biosensor, and detecting the working curve of the sensor for later use; s2, preparing a histamine monoclonal antibody; s3, preparing an enzyme-labeled antibody; s4, detecting histamine by using the sensor. After uniformly mixing an equal volume of a sample solution to be tested and the enzyme-labeled antibody prepared in the step S3, dripping the mixture into an electrode working area, immersing the electrode in a test solution, adding hydrogen peroxide, and quantitatively measuring the histamine concentration according to the change of the front peak current value and the back peak current value.
On the other hand, the method for detecting biogenic amine by adopting the biosensor in CN-104897897 has the advantages of low detection limit, high sensitivity, wide linear range, suitability for field detection and the like. However, the prior art of the method of detecting biogenic amines by this biosensor is still not very mature, the market is less reliable in kit and it is expensive.
Another common technique for detecting biogenic amines is the "portable meat freshness detector" patent of the TW-I467171 patent bulletin of Taiwan area of China, which discloses a portable meat freshness detector. The portable meat freshness detector measures an impedance value and a capacitance value of meat by a sensing unit, and calculates the measured impedance value and capacitance value by an iterative algorithm program arranged in a processing unit to obtain index parameter values; and then, comparing the index parameter value with the standard parameter value recorded in the memory to obtain an operation result of the freshness of the meat, and displaying the operation result on a display unit.
In addition, the TW-I467171 technology for calculating the resistance value and the capacitance value of the surface of the meat by contact measurement and obtaining the freshness of the meat has the advantages of no damage to the meat, rapid detection, convenient operation, etc., but cannot perform contact measurement for the packaged food, so that the freshness of the packaged food or food cannot be confirmed.
Another common biogenic amine detection technology, such as the "Cycloarene functionalized gold nanoparticle and preparation method thereof" of Chinese patent publication No. CN-104148626, is disclosed in the present application, which discloses a Cycloarene functionalized gold nanoparticle and preparation method thereof. Modifying a cyclic aromatic hydrocarbon CEC4 to the surface of a gold nanoparticle by a gold-thiol bond of the gold nanoparticle, wherein the concentration ratio of the cyclic aromatic hydrocarbon CEC4 to the gold nanoparticle is 2000000:23, and the particle size of the gold nanoparticle is 13nm, and CEC4 functionalized gold nanoparticle has specificity in the detection of biogenic amine.
On the other hand, the aforementioned CN-104148626 uses the color change generated by the reaction of the compound with biogenic amine, and further detects the freshness of the food. Although this method has the advantage of simplicity and rapidness in discrimination, it still needs to be in contact with the extract to react, which is a contact or semi-contact measurement method. The contact measurement cannot be performed for the completed packaged food, and thus the freshness of the completed packaged food or food material cannot be confirmed.
Another common technique for detecting biogenic amines is disclosed in U.S. Pat. No. 3, polymeric food spoilage sensor, U.S. Pat. No. 3,379, which discloses a food spoilage (spoilage) detector. The food spoilage (freshness) detector comprises a polymer and the polymer comprises a composite material (polyaza macrocyclic transition metal complex) that releases biogenic amine upon contact with spoilage food microorganisms (microorganisms) and reacts with biogenic amine to produce a color change.
On the other hand, the aforementioned US-6593142 also uses the color change generated by the reaction of the compound with biogenic amine, and further detects the freshness of the food. Although this method has the advantage of simplicity and rapidness in discrimination, it still needs to be in contact with the extract to react, which is a contact or semi-contact measurement method. The contact measurement cannot be performed for the completed packaged food, and thus the freshness of the completed packaged food or food material cannot be confirmed.
Another common biogenic amine adsorption removal technique, such as the "Biogenic amine oxidizer or unreactive absorber" patent application of U.S. patent publication No. US-2014/0087034, discloses a biogenic amine adsorption oxidizer (oxyazer). The biogenic amine adsorption oxidizer is used for adsorbing and removing biogenic amine of food during storage (storage) or packaging (packing) of the food.
On the contrary, the biogenic amine adsorption oxidizer of the aforementioned US-2014/0087034 is only suitable for adsorbing biogenic amine from food or for reducing biogenic amine from food, but is not used for displaying biogenic amine or freshness of food or food materials.
Another common biogenic amine adsorption removal technique, such as the "Sencore cromoge nicos para aminas" patent application of Spanish patent publication ES-2557332, discloses a biogenic amine sensitive material. The membrane M1 and the membrane M1sen of the biogenic amine sensitive material adopt complex components and complex preparation operations.
As a result, the biogenic amine sensitive material of the ES-2557332 is completely free of biogenic amine gas adsorbing substrate and the predetermined adsorption zone thereof, so as to be bonded to biogenic amine sensitive material, and the biogenic amine sensitive material is not used for displaying biogenic amine or freshness of food or food materials.
Another common technique for detecting biogenic amines, such as the "chemical sensing hydrogel for biogenic amine detection" patent application of chinese patent publication No. CN-107037044, discloses a chemical sensing hydrogel for biogenic amine detection, i.e. it belongs to a wet hydrogel or a wet hydrogel mixture, which needs to be combined with a container for packaging food in a sealing manner.
On the other hand, the wet hydrogel of the chemical sensing hydrogel for biogenic amine detection of CN-107037044 has the disadvantage of complex processing process, the wet hydrogel does not have biogenic amine gas adsorption substrate and its predetermined adsorption area, and the biogenic amine sensitive material does not show biogenic amine or freshness of food or food materials with dry substrate.
However, there is a need for further improvement in the detection devices and methods of the aforementioned Chinese patent publication No. CN-102650628, chinese patent publication No. CN-103149317, chinese patent publication No. CN-104897897, taiwan patent publication No. TW-I467171, chinese patent publication No. CN-104148626, U.S. Pat. No. US-6593142, U.S. Pat. No. US-2014/0087034, spanish patent publication No. ES-2557332 and Chinese patent publication No. CN-107037044.
The foregoing chinese patent publication nos. CN-102650628, CN-103149317, CN-104897897, TW-I467171, CN-104148626, US-6593142, US-2014/0087034, ES-2557332 and CN-107037044 are merely references to the technical background of the present invention and are not intended to limit the scope of the present invention.
In view of the above, the present invention provides a method and apparatus for detecting biogenic amine in food materials, which comprises disposing a biogenic amine sensitive material on a biogenic amine gas adsorption substrate to form a biogenic amine detecting element, and combining the biogenic amine detecting element with a food material to be detected or a food material to be detected, wherein the biogenic amine sensitive material is utilized to generate and display a biogenic amine index once the biogenic amine detecting element contacts a food material biogenic amine vaporization gas or a biogenic amine vapor, so as to improve the technical shortcomings of the conventional method and apparatus for detecting biogenic amine in food materials.
Disclosure of Invention
The main objective of the present invention is to provide a method and a device for detecting biogenic amine in food materials, which is to dispose a biogenic amine sensitive material on a biogenic amine gas adsorption substrate to form a biogenic amine detection element, and combine the biogenic amine detection element with a food material package to be detected or a food material to be detected, and once the biogenic amine detection element contacts a food material biogenic amine vaporization gas or a biogenic amine vapor, the biogenic amine sensitive material is immediately utilized to generate and display a biogenic amine index, so as to achieve the purposes of simplifying the operation of detecting the biogenic amine in food materials, accelerating the detection efficiency and reducing the detection cost.
In order to achieve the above object, a device for detecting biogenic amine on food material according to a preferred embodiment of the present invention comprises:
a biogenic amine gas adsorption substrate having a predetermined adsorption zone, wherein the predetermined adsorption zone of the biogenic amine gas adsorption substrate is used for adsorbing a biogenic amine vaporization gas or a biogenic amine vapor;
a biogenic amine sensitive material disposed on the biogenic amine gas adsorption substrate to form a biogenic amine detection element; a kind of electronic device with high-pressure air-conditioning system
An element combining part which is arranged on the biogenic amine detection element and is combined on a food to be detected or a food to be detected package;
wherein the biogenic amine sensing material is utilized to generate a sensor that displays a biogenic amine index [ color change ] upon contact of the biogenic amine sensing element with a food material biogenic amine boil-off gas or biogenic amine vapor.
The biogenic amine gas adsorbing substrate according to the preferred embodiment of the present invention is selected from a water absorbing substrate or a bio-compatible substrate.
The biogenic amine gas adsorbing substrate of the preferred embodiment of the present invention is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof.
The biogenic amine sensitive material of the preferred embodiment of the present invention comprises a shaping layer and a color changing layer.
The biogenic amine sensitive material of the preferred embodiment of the present invention is selected from mononitrated benzene ring molecular materials.
The nitrated benzene ring molecular material of the preferred embodiment of the invention has one nitrated functional group, two nitrated functional groups or three nitrated functional groups.
In order to achieve the above object, another preferred embodiment of the method for detecting biogenic amine in food material of the present invention comprises:
providing a biogenic amine detecting element, wherein the biogenic amine detecting element comprises a biogenic amine gas adsorption substrate, and the biogenic amine gas adsorption substrate is used for adsorbing biogenic amine vaporization gas or biogenic amine vapor;
disposing a biogenic amine sensitive material on the biogenic amine gas adsorption substrate;
combining the biogenic amine detection element with a to-be-detected food material package or a to-be-detected food material; a kind of electronic device with high-pressure air-conditioning system
Once the biogenic amine detection element contacts a food biogenic amine boil-off gas or biogenic amine vapor, the biogenic amine sensitive material is immediately utilized to produce a sensor that displays a biogenic amine index [ color change ].
The biogenic amine index of the preferred embodiment of the present invention is a biogenic amine concentration, for example: 50ppm, 100ppm, 500ppm, 1000ppm or other concentrations.
The biogenic amine concentration of the preferred embodiment of the present invention comprises a biogenic amine safe concentration, a biogenic amine alert concentration, or a biogenic amine hazard concentration.
The safety concentration of the biogenic amine in the preferred embodiment of the invention is lower than 20ppm, the warning concentration of biogenic amine is 20-50ppm, and the hazard concentration of biogenic amine is higher than 50ppm.
The biogenic amine index of the preferred embodiment of the present invention is a visible light color change indicator, such as: the primary color is green, and more than 50ppm is dark red or orange brown.
The biogenic amine detection element according to the preferred embodiment of the present invention generates a biogenic amine index at least within 1 hour, or preferably generates the biogenic amine index at least within 30 minutes, or more preferably generates the biogenic amine index at least within 10 minutes, or more preferably generates the biogenic amine index at least within 5 minutes, or more preferably generates the biogenic amine index at least within 1 minute when contacting food biogenic amine vaporization gas or biogenic amine vapor.
The biogenic amine detection element of the preferred embodiment of the invention comprises an element combination part, and the element combination part is combined on a food material package to be detected or a food material to be detected.
The element coupling portion of the preferred embodiment of the present invention is an adhesive portion, a label patch, a coupling clasp, a label clasp, or other structural coupling portion.
The invention has the beneficial effects that:
the invention provides a device and a method for detecting food material biogenic amine, which are characterized in that a biogenic amine sensitive material is arranged on a biogenic amine gas adsorption substrate to form a biogenic amine detection element, the biogenic amine detection element is combined on a food material package to be detected or a food material to be detected, and once the biogenic amine detection element contacts a food material biogenic amine vaporization gas or a biogenic amine vapor, the biogenic amine sensitive material is immediately utilized to generate and display a biogenic amine index, so that the purposes of simplifying the operation of detecting the food material biogenic amine, accelerating the detection efficiency and reducing the detection cost are achieved.
Drawings
Fig. 1 is a schematic side view of a device for detecting biogenic amine on food materials according to a first preferred embodiment of the present invention.
Fig. 2 is a flow chart of a method for detecting biogenic amine in food materials according to a preferred embodiment of the present invention.
Fig. 3 is a schematic side view of a device for detecting biogenic amine on food material according to a second preferred embodiment of the present invention.
Fig. 4 is a schematic side view of a device for detecting biogenic amine on food materials according to a third preferred embodiment of the present invention.
FIG. 5 is a schematic diagram of a device for detecting biogenic amine in food material according to the preferred embodiment of the present invention, which uses biogenic amine sensitive material to act on biogenic amine.
FIG. 6 is a schematic diagram of the chemical structure of the apparatus for detecting biogenic amine in food material according to the preferred embodiment of the present invention using biogenic amine sensitive material and biogenic amine mechanism.
Fig. 7A is a schematic chemical structure diagram of a conjugated molecule of a first sensitive material used in a device for detecting biogenic amine on food materials according to a preferred embodiment of the present invention.
FIG. 7B is a schematic chemical structure of a conjugated molecule of a second sensitive material used in the apparatus for detecting biogenic amine on food materials according to the preferred embodiment of the present invention.
FIG. 7C is a schematic chemical structure of a conjugated molecule of a third sensitive material used in the apparatus for detecting biogenic amine on food materials according to the preferred embodiment of the present invention.
Fig. 8A is a schematic chemical structure diagram of a device for detecting biogenic amine in food material using a first control molecular material according to a preferred embodiment of the present invention.
Fig. 8B is a schematic chemical structure diagram of a device for detecting biogenic amine in food material using a second control molecular material according to a preferred embodiment of the present invention.
Reference numerals
1: biogenic amine gas adsorption substrates; 10: a device for detecting biogenic amine gas of food materials; 11: a component joining portion; 11a: combining a retaining ring; 2: a biogenic amine sensitive material; 3: an adhesive layer; g1: a sensitive material; and G2: controlling the molecular material; s1: a step of; s2: a step of; s3: a step of; s4: and (3) step (c).
Detailed Description
For a thorough understanding of the present invention, reference will now be made in detail to the preferred embodiments, examples of which are illustrated in the accompanying drawings and are not intended to limit the invention.
The method and the device for detecting the biogenic amine in the food material can be properly selected to automatically, semi-automatically or non-automatically process or be implemented on the surfaces of various agricultural, forestry, fishery, pasture products, processed products or byproducts thereof; furthermore, the apparatus for detecting biogenic amine in food material according to the preferred embodiment of the present invention can be suitably applied to various related agricultural, forestry, fishery, pasture products, processed products thereof, byproducts thereof or related industries, but is not intended to limit the application scope of the present invention.
For example, the method and apparatus for detecting biogenic amine in food materials according to the preferred embodiments of the present invention are suitable for various vegetable and fruit products (such as sweet potato leaf, water spinach, green pepper, watermelon, strawberry, mango, pineapple, grape, papaya, sweet potato, radish, carrot, eggplant, red pepper or other vegetable and fruit products), agricultural products (such as pericarp surface of agricultural products, outer skin of beans, nut shells, surface of starter block or other agricultural products), or a fresh product (such as meat, seafood or other fresh products), but are not limited to the application scope of the present invention.
In the preferred embodiment of the present invention, the definition of "biogenic amine" or "biogenic amine" includes amines emitted when food materials such as "histamine", "putrescine" or "cadaverine" are putrefied, and the definition of "biogenic amine gas" or "biogenic amine vaporization gas" is defined as "biogenic amine vapor", "biogenic amine vaporization gas" or "biogenic amine vaporization gas", but it is not intended to limit the scope of the present invention.
Fig. 1 is a schematic side view of a device for detecting biogenic amine on food materials according to a first preferred embodiment of the present invention. Referring to fig. 1, a device 10 for detecting biogenic amine gas in a food material according to a first preferred embodiment of the present invention comprises a biogenic amine gas adsorption substrate 1, a biogenic amine sensitive material 2 and a component combining portion 11, and the biogenic amine gas adsorption substrate 1, the biogenic amine sensitive material 2 and the component combining portion 11 are properly combined to form the device 10 for detecting biogenic amine gas in the food material.
Referring to fig. 1 again, for example, the biogenic amine gas adsorbing substrate 1 comprises a first surface (or upper surface, not shown) and a second surface (or lower surface, not shown). The biogenic amine gas adsorption substrate 1 has a predetermined adsorption zone (e.g. an open gas contact zone), and the predetermined adsorption zone of the biogenic amine gas adsorption substrate 1 is suitable for adsorbing a biogenic amine vaporization gas (i.e. biogenic amine vaporization gas) or a biogenic amine vapor (i.e. biogenic amine vapor).
Referring to fig. 1 again, for example, the biogenic amine gas adsorbing substrate 1 can be selected from a water absorbing substrate or a biocompatible substrate. Alternatively, the biogenic amine gas adsorbing substrate 1 may be made of a pulp material, a cotton material, a sponge material, a textile, or any combination thereof (or other suitable material having a water absorbing function or a function of adsorbing water vapor), or a dry substrate (e.g., filter paper, cotton sheet or other suitable dry substrate, i.e., non-wet hydrogel substrate) to assist in adsorbing the biogenic amine vaporized gas.
Referring to fig. 1 again, for example, the biogenic amine sensitive material 2 is disposed on any suitable location (e.g., disposed in a suitable manner) on the first surface of the biogenic amine gas adsorbing substrate 1 to form a biogenic amine detecting device or a device with biogenic amine detecting function. The biogenic amine sensitive material 2 may optionally form a biogenic amine sensitive layer, and the biogenic amine sensitive layer comprises a first surface (or upper surface, not labeled) and a second surface (or lower surface, not labeled).
Referring to fig. 1 again, for example, the biogenic amine sensitive material 2 is a sensitive material that can react with biogenic amine, and the biogenic amine sensitive material 2 comprises a shaping layer and a color-changing layer (such as color-changing region, color-changing pattern, color-changing text or other color-changing design), and the color-changing layer comprises a biogenic amine color-changing material. In addition, the biogenic amine sensitive material 2 can be selected from a nitrated benzene ring molecular material or other various chemical substances which can be combined and reacted with biogenic amine, and the nitrated benzene ring molecular material has one nitrated functional group, two nitrated functional groups, three nitrated functional groups or other materials with similar functional groups.
Referring to fig. 1 again, for example, the element connecting portion 11 is disposed on the biogenic amine detecting element, and the element connecting portion 11 is connected to a food to be detected or a packaging of the food to be detected (for example, a suitable sleeve or a suitable set of the food to be detected). In addition, the element bonding portion 11 may be optionally disposed at any suitable position on the first surface or the second surface of the biogenic amine gas adsorbing substrate 1.
Fig. 2 shows a flow chart of a method for detecting biogenic amine in food material according to a preferred embodiment of the present invention, which includes four main steps S1 to S4, but is not limited to the sequence of steps of the present invention, and the sequence of steps of the preferred embodiment of the present invention can be changed, divided, added, combined or reduced as appropriate without departing from the scope of the present invention.
Referring to fig. 1 and 2, a method for detecting biogenic amine in food material according to a preferred embodiment of the present invention includes the steps of S1: first, for example, one or several biogenic amine detecting elements are provided in a proper manner, and the biogenic amine detecting elements comprise the biogenic amine gas adsorbing base material 1, and the biogenic amine gas adsorbing base material 1 is used for adsorbing a biogenic amine vaporization gas.
Referring to fig. 1 and 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention comprises the following steps: next, the biogenic amine sensitive material 2 may be optionally pre-disposed (e.g., dip coated, spray coated, drop coated or otherwise) on the biogenic amine gas adsorbing substrate 1 in a suitable manner, for example, to detect freshness of the food material to be tested, its associated reference index or other detection reference index.
Referring to fig. 1 and 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention comprises the following steps: then, for example, the biogenic amine detection element can be selectively combined with any food package to be tested (for example, the inner surface of the package is not shown) or any food to be tested (for example, the biogenic amine detection element is attached to any part of the food) in the food processing operation or the food packaging operation.
Referring to fig. 1 and 2 again, the method for detecting biogenic amine in food material according to the preferred embodiment of the present invention comprises the following step S4: then, for example, upon contact of the biogenic amine detecting element with a food biogenic amine vaporizing gas or a biogenic amine vapor, immediately utilizing the biogenic amine sensitive material 2 produces a biogenic amine index (color change) which is visually recognizable change. The method for detecting biogenic amine in the preferred embodiment of the invention does not need to touch the biogenic amine or exuded liquid thereof, but only needs to absorb biogenic amine vaporization gas emitted by the food to be detected to detect the biogenic amine in the food.
Referring again to fig. 1 and 2, for example, the biogenic amine index may be selected to be visibly distinguishable from the change, such as: color change, transparency change, display of warning signs, display of warning text or other identifiable physical or chemical changes.
Referring again to fig. 1 and 2, for example, the biogenic amine index may be optionally set to a biogenic amine concentration, such as: 50ppm, 100ppm, 500ppm, 1000ppm or other concentrations. For example, the U.S. Food and Drug Administration (FDA) prescribes that the histamine content of aquatic products must not exceed 50ppm and that once the histamine content reaches 500ppm, it is terrible to the health of humans.
Referring to fig. 1 and 2, the biogenic amine concentration includes a biogenic amine safety concentration, a biogenic amine warning concentration, or a biogenic amine hazard concentration, for example. The biogenic amine safe concentration is lower than 20ppm, the biogenic amine warning concentration is 20-50ppm, and the biogenic amine hazard concentration is higher than 50ppm. The biogenic amine index is a visible light color change indication, such as: the primary color green, over 50ppm is dark red or orange brown, but this is not intended to limit the scope of the invention.
For example, if the biogenic amine concentration of the food to be measured is close to 50ppm (i.e. the biogenic amine warning concentration), the food to be measured can be selected as a lower frame food, and the lower frame food is further processed into another processed food or processed food, so as to reduce improper waste of food caused by exceeding the biogenic amine concentration of the food to be measured.
The biogenic amine detection element according to the preferred embodiment of the present invention generates a biogenic amine index at least within 1 hour, or preferably generates the biogenic amine index at least within 30 minutes, or more preferably generates the biogenic amine index at least within 10 minutes, or more preferably generates the biogenic amine index at least within 5 minutes, or more preferably generates the biogenic amine index at least within 1 minute when contacting food biogenic amine vaporization gas or biogenic amine vapor.
Fig. 3 is a schematic side view of a device for detecting biogenic amine on food materials according to a second preferred embodiment of the present invention. Referring to fig. 3, in comparison with the first embodiment, the apparatus for detecting biogenic amine in the second preferred embodiment of the present invention selectively provides at least one adhesive layer 3 on the second surface (or the lower surface) or other positions (the first surface or the upper surface) of the biogenic amine gas adsorbing substrate 1.
Referring to fig. 3 again, for example, in comparison with the second embodiment, the adhesive layer 3 is optionally disposed on the first surface (or the upper surface) of the biogenic amine sensitive material 2, and the biogenic amine gas adsorbing substrate 1 is used for adsorbing biogenic amine gas, biogenic amine vaporization gas or biogenic amine vapor.
Fig. 4 is a schematic side view of a device for detecting biogenic amine on food materials according to a third preferred embodiment of the present invention. Referring to fig. 4, in comparison with the first embodiment, the apparatus for detecting biogenic amine as a food material according to the third preferred embodiment of the present invention is provided with a combining buckle 11a at one side of the biogenic amine gas absorbing substrate 1.
Referring to fig. 1 and 4, for example, the element combining portion 11 can be selected as an adhesive portion, a label patch (suitable for various non-aqueous food materials), a label buckle (suitable for various aquatic products or various meats) or other structural combining portions according to various different requirements.
FIG. 5 is a schematic diagram showing the operation of the apparatus for detecting biogenic amine on food material using biogenic amine sensitive material according to the preferred embodiment of the present invention. Referring to fig. 1 and 5, for example, the biogenic amine sensitive material 2 includes a sensitive material G1, a polymer carrier material and a control molecular material G2, and a predetermined blending ratio is provided between the sensitive material G1 and the control molecular material G2, so that the biogenic amine sensitive material 2 adjusts and controls a color change range according to the detected biogenic amine concentration.
Referring to fig. 1 and 5 again, for example, the sensitive material G1 has a reactive group capable of reacting with biogenic amine and an organic color-changing group (i.e. conjugated portion) containing conjugated double bonds. The control molecular material G2 is used for controlling environmental values, and the control molecular material G2 has a characteristic of being capable of combining with biogenic amine (competing reaction), so as to control the color-changing concentration, as shown in the upper half of fig. 5. In addition, the polymeric carrier material is suitable for dip coating or spray coating the biogenic amine sensitive material 2 on filter paper, cotton wool or other suitable dry substrate of the biogenic amine gas adsorbing substrate 1.
Referring to fig. 5 again, for example, after detecting biogenic amine on the food material, biogenic amine (steam or gas) is first combined with the reactive group of the sensitive material G1, as shown in the left side of fig. 5, electrons are transferred to the conjugated portion through the reactive group to generate a color change reaction, as shown in the middle portion of fig. 5, so that the conjugated double bond structure of the organic color change group is changed to finally cause a color change (for example, to change a predetermined color or change brightness), as shown in the right side of fig. 5.
Fig. 6 is a schematic chemical structure diagram of a device for detecting biogenic amine in food material according to a preferred embodiment of the present invention, which is corresponding to fig. 5, using biogenic amine sensitive material and biogenic amine action mechanism. Referring to fig. 5 and 6, for example, when a substitution or addition reaction (as shown in the left side of fig. 6) is performed between biogenic amine (steam or gas) and the conjugated portion, the conjugated double bond conduction of the conjugated portion of the sensitive material G1 promotes the conjugated portion to generate color change, the control molecule of the control molecule material G2 can control the color change range of the conjugated portion, and the polymer carrier material can adsorb volatile amine, and fix the color change molecule of the conjugated portion and fix the control molecule.
Fig. 7A is a schematic chemical structure diagram of a conjugated molecule of a first sensitive material G1, which corresponds to the conjugated portion of fig. 5, used in the apparatus for detecting biogenic amine in food according to the preferred embodiment of the present invention. Fig. 7B is a schematic chemical structure diagram of a conjugated molecule of a second sensitive material G1, which corresponds to the conjugated portion of fig. 5, used in the apparatus for detecting biogenic amine in food according to the preferred embodiment of the present invention. Fig. 7C is a schematic chemical structure diagram of a conjugated molecule of a third sensitive material G1, which corresponds to the conjugated portion of fig. 5, used in the apparatus for detecting biogenic amine in food according to the preferred embodiment of the present invention.
Fig. 8A is a schematic chemical structure diagram of a device for detecting biogenic amine in food material according to a preferred embodiment of the present invention, which is a control molecular material corresponding to the control molecular material of fig. 5, using a first control molecular material G2. Fig. 8B is a schematic chemical structure diagram of a device for detecting biogenic amine in food material according to a preferred embodiment of the present invention, which is a second control molecular material G2, and corresponds to the control molecular material of fig. 5.
The foregoing description of the preferred embodiments merely illustrates the invention and its technical features, and the technology of this embodiment can be implemented in various substantially equivalent modifications and/or alternative ways as appropriate; the scope of the invention should, therefore, be determined with reference to the appended claims.
Claims (10)
1. A device for detecting biogenic amines in food material, comprising:
a biogenic amine gas adsorption substrate having a predetermined adsorption zone, wherein the predetermined adsorption zone of the biogenic amine gas adsorption substrate is used for open adsorption of a biogenic amine vaporization gas or a biogenic amine vapor;
the biogenic amine sensitive material is arranged on the biogenic amine gas adsorption substrate to form a biogenic amine detection element, the biogenic amine sensitive material comprises a sensitive material, a high polymer carrier material and a control molecular material, the sensitive material is provided with a reactive group capable of reacting with biogenic amine and an organic color-changing group containing conjugated double bonds, after the biogenic amine vaporization gas or biogenic amine vapor is combined with the reactive group capable of reacting with biogenic amine, electrons of the biogenic amine vapor are transferred to the organic color-changing group containing conjugated double bonds through the reactive group capable of reacting with biogenic amine to generate color-changing reaction, and the control molecular material is combined with biogenic amine to further control color-changing concentration; a kind of electronic device with high-pressure air-conditioning system
An element combining part which is arranged on the biogenic amine detection element and is combined on a food to be detected or a food to be detected package;
wherein the biogenic amine sensitive material is utilized to generate a color change as soon as a predetermined adsorption area of the biogenic amine gas adsorption substrate of the biogenic amine detection element is in open contact with a food biogenic amine vaporization gas or biogenic amine vapor.
2. The apparatus for detecting food biogenic amine according to claim 1, wherein said biogenic amine gas absorbing substrate is selected from a water absorbing substrate and a bio-compatible substrate.
3. The device for detecting biogenic amines as claimed in claim 1, wherein said biogenic amine gas absorbing substrate is made of a pulp material, a cotton material, a sponge material, a textile or any combination thereof.
4. The apparatus for detecting food biogenic amine as claimed in claim 1, wherein the biogenic amine sensitive material comprises a shaping layer and a color changing layer.
5. The apparatus for detecting food material biogenic amine according to claim 1, wherein the biogenic amine sensitive material is selected from mononitrated benzene ring molecular materials.
6. A method for detecting biogenic amines in food materials, comprising:
providing a biogenic amine detecting element, wherein the biogenic amine detecting element comprises a biogenic amine gas absorbing substrate, and a preset absorbing area of the biogenic amine gas absorbing substrate is used for absorbing a biogenic amine vaporization gas or biogenic amine vapor in an open mode;
a biogenic amine sensitive material is arranged on the biogenic amine gas adsorption substrate, the biogenic amine sensitive material comprises a sensitive material, a high polymer carrier material and a control molecular material, the sensitive material is provided with a reactive group capable of reacting with biogenic amine and an organic color-changing group containing conjugated double bonds, after the biogenic amine vaporization gas or biogenic amine vapor is combined with the reactive group capable of reacting with biogenic amine, electrons of the biogenic amine vapor are transferred to the organic color-changing group containing conjugated double bonds through the reactive group capable of reacting with biogenic amine to generate color-changing reaction, and the control molecular material is combined with biogenic amine to further control color-changing concentration;
combining the biogenic amine detection element with an element combining part on a food material package to be detected or a food material to be detected; a kind of electronic device with high-pressure air-conditioning system
Once the prearranged adsorption area of the biogenic amine gas adsorption substrate of the biogenic amine detection element is in open contact with a food biogenic amine vaporization gas or biogenic amine vapor, the biogenic amine sensitive material is immediately utilized to generate a color change.
7. The method of claim 6, wherein the biogenic amine index is a biogenic amine concentration.
8. The method of claim 7, wherein the biogenic amine concentration comprises a biogenic amine safety concentration, a biogenic amine warning concentration, or a biogenic amine hazard concentration.
9. The method of claim 6, wherein the biogenic amine detection element comprises an element bonding portion, and the element bonding portion is bonded to a food packaging or a food to be tested.
10. The method of claim 9, wherein the component attachment portion is an adhesive portion, a label patch, an attachment clasp, or a label clasp.
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