CN112300966A - Preparation method of high-activity lactic acid bacteria - Google Patents

Preparation method of high-activity lactic acid bacteria Download PDF

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CN112300966A
CN112300966A CN202011226633.6A CN202011226633A CN112300966A CN 112300966 A CN112300966 A CN 112300966A CN 202011226633 A CN202011226633 A CN 202011226633A CN 112300966 A CN112300966 A CN 112300966A
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孙震颖
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Abstract

The invention discloses a method for preparing high-activity lactobacillus, which comprises the steps of preparing a culture medium, pretreating the culture medium, preheating a culture tank, activating and adding strains, culturing the lactobacillus, carrying out enlarged culture of the lactobacillus and disinfecting and cleaning, wherein the top end of a substrate is fixedly provided with an incubator, the top end of the substrate is fixedly provided with a cleaning box corresponding to one side of the incubator, one end of the incubator is embedded with a control switch, and the top end of the incubator is provided with a deodorizing top cover. The total time consumption of culturing different kinds of lactobacillus is reduced, and the economy of culturing lactobacillus is improved.

Description

Preparation method of high-activity lactic acid bacteria
Technical Field
The invention relates to the technical field of lactobacillus preparation, in particular to a method for preparing high-activity lactobacillus.
Background
Lactic acid bacteria are a general name of bacteria which can utilize fermentable carbohydrates to generate a large amount of lactic acid, the bacteria are very widely distributed in the nature and have abundant species diversity, except for a very small number, most of the lactic acid bacteria are flora which are indispensable in the human body and have important physiological functions, and the lactic acid bacteria widely exist in the intestinal tract of the human body, not only are the ideal materials for research classification, biochemistry, heredity, molecular biology and genetic engineering, have important academic values in theory, but also have extremely high application values in important fields closely related to human life, such as industry, agriculture, animal husbandry, food, medicine and the like;
when the existing preparation method of the lactobacillus acidophilus is implemented, a solid culture medium adopted by the lactobacillus acidophilus is generally in a large block shape, and needs to be crushed by a worker in advance by using an apparatus, and the crushed solid culture medium still contains large particles, so that the cultivation efficiency and the cultivation effect of the lactobacillus acidophilus are easily influenced, and the quality of the prepared lactobacillus acidophilus cannot reach the standard.
Disclosure of Invention
The technical scheme provided by the invention can effectively solve the problem that the quality of prepared lactic acid bacteria cannot reach the standard due to the fact that the solid culture medium adopted by lactic acid bacteria in the background technology is generally in a large block shape, and a worker needs to use an instrument to crush the lactic acid bacteria in advance, and the crushed solid culture medium still contains large particles, so that the cultivation efficiency and the cultivation effect of the lactic acid bacteria are easily affected.
In order to achieve the purpose, the invention provides the following technical scheme: a method for preparing high-activity lactic acid bacteria comprises the following steps:
s1, preparation of a culture medium: selecting an MRS solid culture medium, grinding by using a grinding and feeding mechanism, and adding the ground MRS solid culture medium and additives into a culture bin to prepare a culture medium;
s2, pretreatment of a culture medium: homogenizing the prepared culture medium to uniformly mix the solid culture medium in the culture medium with the additive;
s3, preheating of culture tank: using a layered water bath heat preservation mechanism above the top end of the substrate to carry out layered preheating on the culture tank;
s4, strain activation and addition: selecting a lactic acid bacteria strain for activation, then adding the activated strain into a culture tank, and deodorizing and discharging gas in the culture tank by using a deodorizing and discharging mechanism;
s5, culturing lactic acid bacteria: culturing the strain in a culture tank at constant temperature, and performing shaking culture on the strain in the culture tank by using a stable shaking mechanism;
s6, lactic acid bacteria amplification culture: taking out half of the culture medium in the culture tank, and adding an equal amount of newly prepared culture medium again for amplification culture;
s7, disinfection and cleaning: the lactic acid bacteria cultured in the culture tank are taken out, and the culture tank is cleaned and sterilized by a cleaning and sterilizing mechanism.
And S3, performing constant-temperature culture on the strain in the culture tank, and performing shaking culture on the strain in the culture tank by using a stable shaking mechanism, wherein the constant-temperature culture temperature range of the strain in the culture tank is 35-42 ℃, and the constant-temperature culture time of the strain in the culture tank is 18-36 h.
Compared with the prior art, the invention has the beneficial effects that: the invention has scientific and reasonable structure and safe and convenient use:
1. through the layered water bath heat preservation mechanism, the heated water can be transmitted to the water inlet cavity and the water outlet cavity separated from the cavity to exchange heat with the culture tank, the culture tank can be preheated by regions, the water of the heat exchange can be recycled, the utilization rate of the water is improved, the partition plates and the plurality of placing ring plates are matched, different types of lactic acid bacteria can be cultured by regions at constant temperature, the flow rate during the water heat exchange transmission is reduced, the stability of the culture tank during the lactic acid bacteria culture is improved, the stability of a culture bin during the lactic acid bacteria culture can be improved by combining the clamping springs and the clamping arc plates, the condition that the culture amount of the lactic acid bacteria is reduced due to the overhigh flow rate of the water during the lactic acid bacteria culture is reduced, the applicability of the lactic acid bacteria culture is wider, the efficiency and the effect during the lactic acid bacteria culture are improved, and the total time consumption during the, thereby improving the economy of the lactobacillus culture.
2. Through the stable mechanism that rocks that sets up, can be when the lactic acid bacteria is cultivated, rock the culture tank, the even degree of bacterial in the culture medium when can improving the lactic acid bacteria and cultivate, thereby increase the quantity that the bacterial was cultivated in the culture medium, reduce because of the bacterial distributes inhomogeneously, lead to the condition that the bacterial cultivation efficiency reduces, the stable spring of cooperation installation with stabilize the limiting plate, combine the connection soft area of stabilizing limiting plate one end, can rock the culture tank when, stabilize the buffering to rocking of culture tank, can increase the culture tank and rock the length of time when changing the angle, reduce the impact force to the culture medium when the culture tank rocks, thereby improve the stability that the culture tank rocked, output when increasing the lactic acid bacteria and cultivating.
3. The odor removal and emission mechanism can absorb harmful substances and water vapor in gas generated in the preparation of lactic acid bacteria and reduce the emission of the harmful gas generated in the preparation of lactic acid bacteria, so that the peculiar smell generated in the preparation of lactic acid bacteria is avoided, the influence of the harmful substances in the gas generated in the preparation of lactic acid bacteria on the health of workers is also avoided, the environmental protection and emission reduction of the preparation of lactic acid bacteria are ensured, and the absorption partition plate and the active carbon plate which are installed in a matched manner can reduce the absorption liquid overflowing when gas is introduced into the absorption bin, reduce the waste of the hand sanitizer when the harmful substances in the gas are absorbed, improve the contact area of the gas and the active carbon plate and improve the drying effect of the gas absorbed in the odor removal bin;
in conclusion, through the cooperation of layered water bath heat preservation, lactobacillus shaking cultivation and gas absorption drying, the uniformity degree of lactobacillus cultivation can be improved, the efficiency and effect of lactobacillus cultivation are increased, the yield of lactobacillus cultivation is ensured, the influence of harmful substances in gas generated in lactobacillus preparation on the health of workers is reduced, and the economic benefit of lactobacillus preparation is increased.
4. Through the cleaning and disinfecting mechanism who sets up, can be for the preparation of lactic acid bacteria makes disinfection preparation next time, aseptic nature when having guaranteed to cultivate the storehouse and using, it disinfects to cultivate the storehouse to need not the staff to use other disinfection instrument, the probability of adhering to the germ after also having reduced to cultivate the storehouse and taking out the use, reduce because of cultivating the influence that the storehouse surface contains the germ to the lactic acid bacteria cultivation production, the dismantlement filter screen and the circulating filtration storehouse of cooperation installation, can filter the back circulation use to abluent moisture, it is extravagant to reduce the moisture when cultivateing the storehouse, wash the required water resource cost of cultivation storehouse when reducing the lactic acid bacteria and cultivating, the economic nature when having improved the lactic acid bacteria and cultivating.
5. Through the arranged grinding and feeding mechanism, the solid culture medium can be ground, the caking condition of the solid culture medium is reduced, the particle size of the solid culture medium can be reduced, the influence on the culture effect of strains due to overlarge particles of the solid culture medium is reduced, the uniformity of the strains cultured in the solid culture medium is improved, the culture efficiency and the culture effect of lactic acid bacteria are improved, the auxiliary material adding bin and the auxiliary material adding pipe which are installed in a matched manner can add other additives into the culture tank together with the solid culture medium, and the splashing of the ground culture medium during discharge can be reduced by combining the installed feeding electric telescopic rod and the installed discharging hose, so that the raw material utilization rate of the culture medium during preparation is improved;
in conclusion, through the partitioned constant-temperature cultivation of the lactic acid bacteria, the cleaning and disinfection of the cultivation bin, the grinding and adding of the solid-state culture medium, the peculiar smell absorption of the prepared lactic acid bacteria and the shaking cultivation of the lactic acid bacteria, the cultivation efficiency and the cultivation effect of the lactic acid bacteria can be improved, the consumed time for preparing various types of lactic acid bacteria is reduced, the economic benefit during the cultivation of the lactic acid bacteria is improved, the overall activity of the prepared lactic acid bacteria is increased, the harmful gas emission during the cultivation of the lactic acid bacteria is reduced, and the environmental protection during the preparation.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic flow diagram of the preparation of lactic acid bacteria according to the invention;
FIG. 2 is a schematic view showing the structure of a lactic acid bacteria culturing tank of the present invention;
FIG. 3 is a schematic view showing the installation structure of the incubator and the washing tank according to the present invention;
FIG. 4 is a schematic structural diagram of the layered water bath heat preservation mechanism of the present invention;
FIG. 5 is a schematic view of the installation structure of the placing ring plate and the culture chamber of the present invention;
FIG. 6 is a schematic view of the installation structure of the water inlet pipe and the water outlet pipe of the present invention;
FIG. 7 is a schematic view of the mounting structure of the return hole and the heating pipe of the present invention;
FIG. 8 is a schematic view of the cleaning and disinfecting mechanism of the present invention;
FIG. 9 is a schematic view of the installation structure of the circulating filter cartridge and the detachable cover of the present invention;
FIG. 10 is a schematic view of the mounting structure of the mounting plate and attachment post of the present invention;
FIG. 11 is a schematic view of the mounting structure for the spring and arc plates of the present invention;
FIG. 12 is a schematic view of the abrasive feeding mechanism of the present invention;
FIG. 13 is a schematic view of the mounting structure of the charging electric telescopic rod and the charging connecting plate of the present invention;
FIG. 14 is a schematic view of the structure of region A of FIG. 13 according to the present invention;
FIG. 15 is a schematic view showing the installation structure of the auxiliary material addition tube and the grinding guide hole according to the present invention;
FIG. 16 is a schematic illustration of the odor elimination drain mechanism of the present invention;
FIG. 17 is a schematic view of the mounting structure of the absorption vent and odor elimination cartridge of the present invention;
FIG. 18 is a schematic view of the mounting structure of the exhaust pump and the exhaust pipe of the present invention;
FIG. 19 is a schematic structural view of the stabilizing and rocking mechanism of the present invention;
FIG. 20 is a schematic view of the installation structure of the shaking electric telescopic rod and the shaking top seat of the present invention;
reference numbers in the figures: 1. a substrate; 2. an incubator; 3. a cleaning tank; 4. a control switch; 5. a odor removal top cover; 6. sterilizing the top cover; 7. a feeding cover;
8. a layered water bath heat preservation mechanism; 801. a culture tank; 802. a cavity; 803. a partition plate; 804. a middle partition plate; 805. a water inlet cavity; 806. a water outlet cavity; 807. placing a ring plate; 808. a culture bin; 809. clamping the telescopic rod; 810. a clamping spring; 811. clamping the arc plate; 812. a fixing buckle; 813. a culture bin cover; 814. adding a cover; 815. a through hole; 816. a water inlet pipe; 817. a water outlet pipe; 818. a reflux bin; 819. a reflux pump; 820. a conductive separator plate; 821. a return orifice; 822. heating a tube;
9. cleaning and disinfecting mechanism; 901. a drive motor; 902. rotating the connecting shaft; 903. a connecting seat; 904. an electric telescopic rod; 905. a clamping plate; 906. inclining the filter screen; 907. a water chute; 908. a collection bin; 909. a circulating filtration bin; 910. disassembling the cover; 911. disassembling the filter screen; 912. a spray pump; 913. a conveying pipe; 914. a dispersion bin; 915. dispersing the spray heads; 916. placing a clamping plate; 917. placing the plate; 918. connecting columns; 919. a rotating plate; 920. rotating the connecting seat; 921. placing a telescopic rod; 922. placing a spring; 923. placing an arc plate; 924. sterilizing the lamp tube;
10. a grinding and feeding mechanism; 1001. grinding the motor; 1002. placing a rack; 1003. grinding the base; 1004. grinding the top seat; 1005. a drive gear; 1006. a driven gear; 1007. a transmission gear chain; 1008. grinding the feeding bin; 1009. grinding the discharge pipe; 1010. a discharge hose; 1011. a discharge joint; 1012. feeding an electric telescopic rod; 1013. feeding connecting plates; 1014. an auxiliary material adding bin; 1015. an auxiliary material adding pipe; 1016. grinding the guide hole;
11. a deodorizing and discharging mechanism; 1101. an air inlet bin; 1102. an air intake duct; 1103. an absorption bin; 1104. an absorbing barrier; 1105. an absorption through hole; 1106. an absorption vent hole; 1107. a smell removal bin; 1108. a deodorizing air inlet; 1109. an activated carbon plate; 1110. an exhaust pump; 1111. an exhaust pipe;
12. a stable shaking mechanism; 1201. a support base pad; 1202. supporting the soft rod; 1203. supporting the top pad; 1204. shaking the base; 1205. shaking the electric telescopic rod; 1206. shaking the top seat; 1207. a stabilizing spring; 1208. stabilizing the limiting plate; 1209. the soft belt is connected.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
Example (b): as shown in fig. 1, the present invention provides a technical solution, a method for preparing high activity lactic acid bacteria, comprising the steps of:
s1, preparation of a culture medium: selecting an MRS solid culture medium, grinding the MRS solid culture medium by using a grinding and feeding mechanism 10, and adding the ground MRS solid culture medium and additives into a culture bin to prepare a culture medium;
s2, pretreatment of a culture medium: homogenizing the prepared culture medium to uniformly mix the solid culture medium in the culture medium with the additive;
s3, preheating of culture tank: a layered water bath heat preservation mechanism 8 above the top end of the substrate 1 is used for carrying out layered preheating on the culture tank;
s4, strain activation and addition: selecting lactobacillus strains for activation, adding the activated strains into a culture tank, and deodorizing and discharging gas in the culture tank by using a deodorizing and discharging mechanism 11;
s5, culturing lactic acid bacteria: culturing the strain in the culture tank at constant temperature, and performing shaking culture on the strain in the culture tank by using a stable shaking mechanism 12;
s6, lactic acid bacteria amplification culture: taking out half of the culture medium in the culture tank, and adding an equal amount of newly prepared culture medium again for amplification culture;
s7, disinfection and cleaning: the lactic acid bacteria cultured in the culture tank are taken out, and the culture tank is cleaned and sterilized by the cleaning and sterilizing mechanism 9.
And S3, performing constant-temperature culture on the strain in the culture tank, and performing shaking culture on the strain in the culture tank by using the stable shaking mechanism 11, wherein the constant-temperature culture temperature range of the strain in the culture tank is 35-42 ℃, and the constant-temperature culture time of the strain in the culture tank is 18-36 h.
As shown in fig. 2-20, an incubator 2 is fixedly mounted on the top end of a substrate 1, a cleaning box 3 is fixedly mounted on the top end of the substrate 1 corresponding to one side of the incubator 2, a control switch 4 is embedded in one end of the incubator 2, an odor removal top cover 5 is mounted on the top end of the incubator 2, a disinfection top cover 6 is mounted on the top end of the cleaning box 3, and a feeding cover 7 is connected to the top end of the odor removal top cover 5;
a layered water bath heat preservation mechanism 8 is arranged in the incubator 2, and the layered water bath heat preservation mechanism 8 comprises an incubator 801, a cavity 802, a partition plate 803, a middle partition plate 804, a water inlet cavity 805, a water outlet cavity 806, a placing ring plate 807, an incubator 808, a clamping telescopic rod 809, a clamping spring 810, a clamping arc plate 811, a fixing buckle 812, an incubator cover 813, an adding cover 814, a through hole 815, a water inlet pipe 816, a water outlet pipe 817, a reflux bin 818, a reflux pump 819, a conduction partition plate 820, a reflux hole 821 and a heating pipe 822;
a culture tank 801 is installed inside the incubator 2, a cavity 802 is formed inside the culture tank 801, partition plates 803 are symmetrically connected to the inner wall of the cavity 802, a middle partition plate 804 is fixedly connected to the bottom end of the cavity 802, the inside of the cavity 802 is partitioned into a water inlet cavity 805 and a water outlet cavity 806 by the middle partition plate 804, a placing ring plate 807 is uniformly connected to the inner wall of the culture tank 801, a culture bin 808 is placed on the inner side of the placing ring plate 807, a clamping telescopic rod 809 is uniformly connected to the inner wall of the culture tank 801, a clamping spring 810 is sleeved on the outer side of the clamping telescopic rod 809, a clamping arc plate 811 is fixedly connected to one end of the clamping telescopic rod 809, fixing buckles 812 are uniformly and fixedly installed at the top end of the placing ring plate 807, a culture bin cover 813 is clamped at the top end of the culture bin cover 808, an adding cover 814 is embedded in the center of the top end of;
the bottom end and the outer side of the culture bin 808 are uniformly connected with water inlet pipes 816 at positions corresponding to the water inlet cavity 805, the bottom end and the outer side of the culture bin 808 are uniformly connected with water outlet pipes 817 at positions corresponding to the water outlet cavity 806, one end of the incubator 2 is connected with a backflow bin 818 at positions corresponding to the water inlet pipes 816 and the water outlet pipes 817, the inner wall of the bottom end of the backflow bin 818 is fixedly connected with a backflow pump 819, the inner wall of the backflow bin 818 is connected with a conduction partition 820 at a position corresponding to the upper part of the backflow pump 819, the top end of the backflow bin 818 is symmetrically provided with backflow holes 821, and the inner wall of the;
the input electric connection commercial power's of control switch 4 output, control switch 4's output electric connection backwash pump 819 and the input of heating pipe 822, be linked together between the intake antrum 805 of every intermediate bottom 804 one end and the play water cavity 806, the outside of placing crown plate 807 and cultivateing canopy 813 all has the thermal-insulated gasket in the cladding, the radian of centre gripping arc board 811 is 30-45, the other end of outlet pipe 817 is connected with the intake end of backwash pump 819, the heat-proof quality of placing crown plate 807 and cultivateing canopy 813 has been increased, it removes to drive centre gripping arc board 811 when being convenient for cultivate storehouse 808 and centre gripping arc board 811 contact.
The cleaning and disinfecting mechanism 9 is arranged inside the cleaning box 3 and the disinfecting top cover 6, and the cleaning and disinfecting mechanism 9 comprises a driving motor 901, a rotating connecting shaft 902, a connecting seat 903, an electric telescopic rod 904, a clamping plate 905, an inclined filter screen 906, a water chute 907, a collecting bin 908, a circulating filter bin 909, a disassembling cover 910, a disassembling filter screen 911, a spraying pump 912, a transmission pipe 913, a dispersing bin 914, a dispersing spray head 915, a placing clamping plate 916, a placing plate 917, a connecting column 918, a rotating plate 919, a rotating connecting seat 920, a placing telescopic rod 921, a placing spring 922, a placing arc plate 923 and a disinfecting lamp tube 924;
a driving motor 901 is arranged at one end of a cleaning box 3, a rotary connecting shaft 902 is embedded and arranged at a position, corresponding to the upper part of the driving motor 901, at one end of the cleaning box 3, a connecting seat 903 is fixedly arranged at one end of the rotary connecting shaft 902, an electric telescopic rod 904 is uniformly connected to the outer side of the connecting seat 903, a clamping plate 905 is fixedly connected to one end of the electric telescopic rod 904, an inclined filter screen 906 is connected to the inner wall at one end of the cleaning box 3, a water chute 907 is arranged at the inner wall at the bottom end of the cleaning box 3, a collecting bin 908 is arranged at a position, corresponding to the inclined filter screen 906, at one end of the cleaning box 3, a circulating filter bin 909 is fixedly arranged at the other end of the cleaning box 3, a detachable cover 910 is embedded and arranged at the bottom end of the circulating filter bin 909, a detachable filter screen 911 is connected to the top, one end of the delivery pipe 913 is connected with a dispersion bin 914 through a circulating filter bin 909, and one end of the dispersion bin 914 is fixedly provided with a dispersion nozzle 915;
the inner wall of the disinfection top cover 6 is fixedly connected with a placing clamping plate 916, the bottom end of the placing clamping plate 916 is clamped with a placing plate 917, the top end of the placing plate 917 is symmetrically and fixedly provided with a connecting column 918, the top end of the connecting column 918 is rotatably provided with a rotating plate 919, the top end of the rotating plate 919 is fixedly connected with a rotating connecting seat 920, the outer side of the rotating connecting seat 920 is uniformly connected with a placing telescopic rod 921, the outer side of the placing telescopic rod 921 is sleeved with a placing spring 922, one end of the placing telescopic rod 921 is fixedly connected with a placing arc plate 923, and the inner wall of the disinfection top;
the output end of the control switch 4 is electrically connected with the input ends of the driving motor 901, the electric telescopic rod 904, the spray pump 912 and the disinfection lamp tube 924, the driving motor 901 is in transmission connection with the rotating connecting shaft 902 through a gear and a gear, one end of the clamping plate 905 is connected with a corrugated rubber pad, the installation angle of the inclined filter screen 906 is 5-15 degrees, a sealing ring is installed at the joint of the detachable cover 910 and the circulating filter bin 909, the sealing performance of the joint of the detachable cover 910 and the circulating filter bin 909 is improved, and impurities filtered out of the surface of the inclined filter screen 906 can flow into the collection bin 908 along the inclined filter screen 906 for collection and treatment.
The top end of the feeding cover 7 is provided with a grinding and feeding mechanism 10, and the grinding and feeding mechanism 10 comprises a grinding motor 1001, a placing rack 1002, a grinding base 1003, a grinding top seat 1004, a driving gear 1005, a driven gear 1006, a transmission toothed chain 1007, a grinding and feeding bin 1008, a grinding and discharging pipe 1009, a discharging hose 1010, a discharging joint 1011, a feeding electric telescopic rod 1012, a feeding connecting plate 1013, an auxiliary material adding bin 1014, an auxiliary material adding pipe 1015 and a grinding guide hole 1016;
a grinding motor 1001 is fixedly installed at the top end of the feeding cover 7, a placing rack 1002 is connected to the top end of the feeding cover 7 corresponding to one side of the grinding motor 1001, a grinding base 1003 is fixedly connected to the top end of the feeding cover 7 corresponding to the lower position of the placing rack 1002, a grinding top seat 1004 is rotatably installed at the top end of the grinding base 1003, a driving gear 1005 is fixedly connected to an output shaft of the grinding motor 1001, a driven gear 1006 is fixedly installed at the outer side of the top end of the grinding top seat 1004, a transmission toothed chain 1007 is installed at the outer sides of the driving gear 1005 and the driven gear 1006 in a meshed manner, a grinding feeding bin 1008 is fixedly installed at the top end of the placing rack 1002, a grinding discharging pipe 1009 is fixedly connected to the bottom end of the grinding base 1003, a discharging hose 1010 is fixedly connected to the bottom end of the grinding discharging pipe 1009, a discharging, the bottom end of the charging electric telescopic rod 1012 is connected with a charging connecting plate 1013, the positions of the top end of the placing frame 1002 corresponding to the two sides of the grinding base 1003 are connected with an auxiliary material adding bin 1014, the bottom end of the auxiliary material adding bin 1014 is fixedly provided with an auxiliary material adding pipe 1015, and the inner wall of the top end of the grinding top seat 1004 is uniformly provided with grinding guide holes 1016;
the output end of the control switch 4 is electrically connected with the input ends of the grinding motor 1001 and the charging electric telescopic rod 1012.
A smell removal discharge mechanism 11 is arranged in the smell removal top cover 5, and the smell removal discharge mechanism 11 comprises an air inlet bin 1101, an air inlet conduit 1102, an absorption bin 1103, an absorption partition 1104, an absorption through hole 1105, an absorption air outlet 1106, a smell removal bin 1107, a smell removal air inlet 1108, an activated carbon plate 1109, an air exhaust pump 1110 and an air exhaust pipe 1111;
an air inlet bin 1101 is fixedly connected to the inner wall of the bottom end of the odor removing top cover 5, air inlet ducts 1102 are connected to two sides of the top end of the air inlet bin 1101, absorption bins 1103 are fixedly mounted on the inner walls of two ends of the odor removing top cover 5, an absorption partition 1104 is connected to the inner wall of one end of the absorption bin 1103, absorption through holes 1105 are uniformly formed in the top end of the absorption partition 1104, and absorption air outlets 1106 are uniformly formed in positions, corresponding to the positions above the absorption partition 1104, at one end of the absorption bin 1103;
the inner walls of two adjacent ends of the odor removal top cover 5 are both connected with an odor removal bin 1107, one end of the odor removal bin 1107 is uniformly and symmetrically provided with odor removal air inlets 1108, the position, corresponding to the odor removal air inlets 1108, of the inner wall of one end of the odor removal bin 1107 is uniformly connected with an activated carbon plate 1109, the position, corresponding to one side of the activated carbon plate 1109, of the inner wall of one end of the odor removal bin 1107 is fixedly provided with an exhaust pump 1110, and the exhaust end of the exhaust pump 1110 is;
the output terminal of the control switch 4 is electrically connected to the input terminal of the exhaust pump 1110.
The inner side of the incubator 2 is provided with a stable shaking mechanism 12 corresponding to the outer side of the culture tank 801, and the stable shaking mechanism 12 comprises a supporting bottom pad 1201, a supporting soft rod 1202, a supporting top pad 1203, a shaking base 1204, a shaking electric telescopic rod 1205, a shaking top base 1206, a stable spring 1207, a stable limiting plate 1208 and a connecting soft belt 1209;
a supporting bottom pad 1201 is uniformly arranged on the inner wall of the bottom end of the incubator 2 corresponding to the outer side of the culture tank 801, a supporting soft rod 1202 is fixedly arranged on the top end of the supporting bottom pad 1201, a supporting top pad 1203 is fixedly connected to the top end of the supporting soft rod 1202, a shaking base 1204 is uniformly connected to the inner wall of the bottom end of the incubator 2 corresponding to one side of the supporting bottom pad 1201, a shaking electric telescopic rod 1205 is rotatably arranged on the top end of the shaking base 1204, a shaking top base 1206 is rotatably arranged on the top end of the shaking electric telescopic rod 1205, stabilizing springs 1207 are uniformly connected to the outer side of the culture tank 801, a stabilizing limiting plate 1208 is connected to the inner wall of the incubator 2 corresponding to the position of the culture tank 801, and a connecting soft belt 120;
control switch 4's output electric connection rocks electric telescopic handle 1205's input, rocks the top of footstock 1206 and support top pad 1203 and all is connected with the bottom of blake jar 801, and the other end of stabilizer spring 1207 and incubator 2's inner wall fixed connection connect the one end of soft area 1209 and the outside fixed connection of blake jar 801, is convenient for rock the footstock 1206 and support top pad 1203 and be connected blake jar 801 and incubator 2.
The working principle and the using process of the invention are as follows: when the lactobacillus preparation method is carried out, firstly, solid culture medium required by lactobacillus preparation can be added into the grinding feeding bin 1008, meanwhile, the culture tank 801 is placed below the feeding cover 7, the added solid culture medium can fall between the grinding base 1003 and the grinding top seat 1004, meanwhile, the grinding motor 1001 is controlled to operate through the control switch 4, the grinding top seat 1004 can be driven to rotate through the transmission of the driving gear 1005, the driven gear 1006 and the transmission gear chain 1007 when the grinding motor 1001 operates, so that the solid culture medium falling between the grinding base 1003 and the grinding top seat 1004 can be ground, the caking condition of the solid culture medium is reduced, the uniform degree of the culture of strains in the solid culture medium is improved, then, the feeding electric telescopic rod 1012 is controlled to operate through the control switch 4, the feeding connecting plate 1013 can be driven to move downwards through the extension of the electric telescopic rod, the discharging connector 1011 can be driven to move downwards to the interior of the culture tank 801, the ground solid culture medium can be discharged along the grinding discharge pipe 1009 and the discharge hose 1010, other additives can be added into the auxiliary material adding bin 1014, and the additives and the solid culture medium fall into the culture tank 801 together through the conduction of the auxiliary material adding pipe 1015, so that splashing of the ground culture medium during discharge can be reduced, and the utilization rate of raw materials during preparation of the culture medium is improved;
then the control switch 4 controls the operation of the reflux pump 819 and the heating pipe 822, the operation of the heating pipe 822 can heat the water in the reflux bin 818, the reflux pump 819 operates to transmit the heated water to the water inlet cavity 805 and the water outlet cavity 806 which are separated from the cavity 802 through the water inlet pipe 816, the heat in the heated water exchanges heat with the culture tank 801, the culture tank 801 can be preheated in different regions by combining the partition plate 803 connected in the cavity 802, the flowing speed of the heat exchange water is reduced, the situations of insufficient heat exchange and shaking of the culture tank 801 caused by the overhigh water flow speed are reduced, the heat exchange water flows back to the reflux bin 818 through the water outlet pipe 817, the heat exchange water can be recycled, the utilization rate of the water can be improved when the water bath heat preservation is carried out on the culture tank 801, and the waste of water resources during the water bath heat preservation is reduced, when the culture bin 808 is placed on the placement ring plate 807 by using the fixing buckle 812, the culture bin 808 can be clamped by the clamping telescopic rod 809, the clamping spring 810 and the clamping arc plate 811, the mounting stability of the culture bin 808 is improved, and different culture bins 808 are subjected to regional constant-temperature culture, so that different lactic acid bacteria can be subjected to regional constant-temperature culture according to actual use requirements, various lactic acid bacteria can be cultured at one time, and the applicability of lactic acid bacteria culture is wider;
when the lactic acid bacteria in the culture tank 801 are cultured, the operation of the exhaust pump 1110 can be realized, the gas in the odor removal top cover 5 is discharged, the gas in the culture tank 2 is pumped into the absorption bin 1103 through the gas inlet bin 1101 and the gas inlet conduit 1102, the harmful substances in the gas entering the absorption bin 1103 can be absorbed through the absorption liquid in the absorption bin 1103, the absorbed gas can be discharged through the absorption through holes 1105 and the absorption air outlet holes 1106 and enters the odor removal bin 1107 through the odor removal air inlet holes 1108, the water vapor in the gas entering the odor removal bin 1107 can be absorbed through the activated carbon plate 1109 arranged on the inner wall of the odor removal bin 1107, the harmful gas and the water vapor generated during the preparation of the lactic acid bacteria can be absorbed, and the emission of the harmful gas generated during the preparation of the lactic acid bacteria is reduced;
when lactic acid bacteria in the culture tank 801 are cultured, the operation of the shaking electric telescopic rod 1205 can be controlled through the control switch 4, the shaking base 1204 and the shaking top base 1206 are combined, the culture tank 801 can be driven to shake when the shaking electric telescopic rod 1205 extends and contracts, when the culture tank 801 shakes, the shaking of the culture tank 801 can be stably buffered through the supporting bottom pad 1201, the supporting soft rod 1202 and the supporting top pad 1203 at the bottom end of the culture tank 801, and the flexible buffer device can increase the time length when the culture tank 801 shakes to change angles and further improve the stability of the shaking of the culture tank 801 by combining the stabilizing spring 1207 and the connecting soft belt 1209 at one end of the stabilizing limiting plate 1208, which are connected to the outer side of the culture tank 801;
after the preparation of the lactobacillus is finished, the culture bin 808 in the culture tank 801 can be taken out, the lactobacillus in the culture bin 808 can be taken out, the culture bin 808 can be placed between clamping plates 905, the electric telescopic rod 904 is controlled to operate by a control switch 4, the electric telescopic rod 904 is contracted, the culture bin 808 can be clamped by the clamping plates 905, then a spray pump 912 is controlled to operate by the control switch 4, the water in the circulating filter bin 909 can be pressurized and then is transmitted to a dispersion bin 914 through a transmission pipe 913, the water is sprayed out through a dispersion nozzle 915, meanwhile, a driving motor 901 is controlled to operate by the control switch 4, a rotating connecting shaft 902 and a connecting seat 903 are driven to rotate through gear and toothed chain transmission, the clamped culture bin 808 can be rotationally sprayed and cleaned, the cleaning uniformity of the culture bin 808 is improved, the cleaned culture bin 808 is taken out, the placing plate 917 is taken down, the culture bin 808 is placed at the top end of a rotating connecting seat 920, can kick-back the power that produces through placing spring 922, use and place arc board 923 and support cultivation storehouse 808 and place, will place board 917 and install in placing cardboard 916 bottom together with the cultivation storehouse 808 that supports and place afterwards, through the operation of control switch 4 control disinfection fluorescent tube 924, can carry out ultraviolet ray disinfection to the cultivation storehouse 808 of placing, thereby can be for next time the lactic acid bacteria preparation ready of disinfecting, reduce because of cultivateing the influence that the 808 surface of storehouse contains the germ to the lactic acid bacteria cultivation production.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A method for preparing high-activity lactic acid bacteria is characterized by comprising the following steps:
s1, preparation of a culture medium: selecting an MRS solid culture medium, grinding by using a grinding and feeding mechanism (10), and adding the ground MRS solid culture medium and additives into a culture bin to prepare a culture medium;
s2, pretreatment of a culture medium: homogenizing the prepared culture medium to uniformly mix the solid culture medium in the culture medium with the additive;
s3, preheating of culture tank: a layered water bath heat preservation mechanism (8) above the top end of the substrate (1) is used for carrying out layered preheating on the culture tank;
s4, strain activation and addition: selecting lactobacillus strains for activation, then adding the activated strains into a culture tank, and deodorizing and discharging gas in the culture tank by using a deodorizing and discharging mechanism (11);
s5, culturing lactic acid bacteria: culturing the strain in the culture tank at constant temperature, and performing shaking culture on the strain in the culture tank by using a stable shaking mechanism (12);
s6, lactic acid bacteria amplification culture: taking out half of the culture medium in the culture tank, and adding an equal amount of newly prepared culture medium again for amplification culture;
s7, disinfection and cleaning: the lactic acid bacteria cultured in the culture tank are taken out, and the culture tank is cleaned and sterilized by a cleaning and sterilizing mechanism (9).
2. The method of claim 1, wherein the strain is cultured in the culture tank at a constant temperature, and the strain is cultured in the culture tank with shaking by the shaking mechanism (11), wherein the temperature range of the strain is 35-42 ℃ and the duration of the strain culture is 18-36 h.
3. The method for preparing highly active lactic acid bacteria according to claim 1, wherein an incubator (2) is fixedly installed on the top of the base plate (1);
a layered water bath heat preservation mechanism (8) is installed inside the incubator (2), and the layered water bath heat preservation mechanism (8) comprises an incubator (801), a cavity (802), a partition plate (803), a middle partition plate (804), a water inlet cavity (805), a water outlet cavity (806), a placement ring plate (807), a culture bin (808), a clamping telescopic rod (809), a clamping spring (810), a clamping arc plate (811), a fixing buckle (812), a culture bin cover (813), an adding cover (814), a through hole (815), a water inlet pipe (816), a water outlet pipe (817), a backflow bin (818), a backflow pump (819), a conduction partition plate (820), a backflow hole (821) and a heating pipe (822);
the utility model discloses a cultivation box, including incubator (2), incubator (801), cavity (802) have been seted up to the inside of incubator (801), the inner wall symmetric connection of cavity (802) has division board (803), the bottom fixedly connected with intermediate bottom (804) of cavity (802), the inside of cavity (802) is separated by intermediate bottom (804) and is intake antrum (805) and play water cavity (806), the inner wall of incubator (801) evenly is connected with places crown plate (807), place the inboard of crown plate (807) and place and cultivate storehouse (808), the inner wall of incubator (801) evenly is connected with centre gripping telescopic link (809), centre gripping spring (810) has been cup jointed in the outside of centre gripping telescopic link (809), the one end fixedly connected with centre gripping arc board (811) of centre gripping telescopic link (809), the even fixed mounting in top of placing crown plate (807) has fixed knot (812), the top end of the culture bin (808) is clamped with a culture bin cover (813), the top end center of the culture bin cover (813) is embedded with an adding cover (814), and through holes (815) are uniformly formed in the position, corresponding to the outer side of the adding cover (814), of the top end of the culture bin cover (813);
the cultivation device is characterized in that the positions, corresponding to the water inlet cavity (805), of the bottom end and the outer side of the cultivation bin (808) are evenly connected with water inlet pipes (816), the positions, corresponding to the water outlet cavity (806), of the bottom end and the outer side of the cultivation bin (808) are evenly connected with water outlet pipes (817), the positions, corresponding to the water inlet pipes (816) and the water outlet pipes (817), of one end of the cultivation box (2) are connected with a backflow bin (818), the inner wall of the bottom end of the backflow bin (818) is fixedly connected with a backflow pump (819), the positions, corresponding to the upper portion of the backflow pump (819), of the inner wall of the backflow bin (818) are connected with conduction partition plates (820), backflow holes (821) are symmetrically formed in the top end of the backflow bin (818), and the positions, corresponding.
4. The method for preparing high-activity lactic acid bacteria according to claim 3, wherein a cleaning tank (3) is fixedly installed at a position corresponding to one side of the incubator (2) at the top end of the substrate (1), a control switch (4) is embedded into one end of the incubator (2), an odor removal top cover (5) is installed at the top end of the incubator (2), a disinfection top cover (6) is installed at the top end of the cleaning tank (3), and a feeding cover (7) is connected to the top end of the odor removal top cover (5);
a water inlet cavity (805) at one end of each middle partition plate (804) is communicated with a water outlet cavity (806), heat insulation gaskets are respectively coated on the outer sides of the placing ring plate (807) and the culture bin cover (813), the radian of the clamping arc plate (811) is 30-45 degrees, and the other end of the water outlet pipe (817) is connected with the water inlet end of the reflux pump (819);
the input end of the control switch (4) is electrically connected with the output end of a mains supply, and the output end of the control switch (4) is electrically connected with the input ends of the reflux pump (819) and the heating pipe (822).
5. A method for preparing highly active lactic acid bacteria according to claim 3, wherein a cleaning and sterilizing means (9) is installed inside the cleaning tank (3) and the sterilizing top cover (6);
the cleaning and disinfecting mechanism (9) comprises a driving motor (901), a rotating connecting shaft (902), a connecting seat (903), an electric telescopic rod (904), a clamping plate (905), an inclined filter screen (906), a water chute (907), a collecting bin (908), a circulating filtering bin (909), a disassembling cover (910), a disassembling filter screen (911), a spraying pump (912), a transmission pipe (913), a dispersing bin (914), a dispersing sprayer (915), a placing clamping plate (916), a placing plate (917), a connecting column (918), a rotating plate (919), a rotating connecting seat (920), a placing telescopic rod (921), a placing spring (922), a placing arc plate (923) and a disinfecting lamp tube (924);
the cleaning device is characterized in that a driving motor (901) is installed at one end of the cleaning box (3), a rotary connecting shaft (902) is installed at a position, corresponding to the upper portion of the driving motor (901), of one end of the cleaning box (3) in an embedded mode, a connecting seat (903) is fixedly installed at one end of the rotary connecting shaft (902), an electric telescopic rod (904) is evenly connected to the outer side of the connecting seat (903), a clamping plate (905) is fixedly connected to one end of the electric telescopic rod (904), an inclined filter screen (906) is connected to the inner wall of one end of the cleaning box (3), a water guide groove (907) is formed in the inner wall of the bottom end of the cleaning box (3), a collecting bin (908) is installed at a position, corresponding to the inclined filter screen (906), a circulating filter bin (909) is fixedly installed at the other end of the cleaning box (3), and a disassembling, the top end of the detachable cover (910) is connected with a detachable filter screen (911), the inner wall of one end of the circulating filter bin (909) is connected with a spraying pump (912) at a position corresponding to one side of the detachable cover (910), the water outlet end of the spraying pump (912) is connected with a delivery pipe (913), one end of the delivery pipe (913) penetrates through the circulating filter bin (909) to be connected with a dispersion bin (914), and one end of the dispersion bin (914) is fixedly provided with a dispersion spray head (915);
cardboard (916) are placed to the inner wall fixedly connected with of disinfection top cap (6), the bottom joint of placing cardboard (916) has places board (917), the top symmetry fixed mounting who places board (917) has spliced pole (918), the top of spliced pole (918) is rotated and is installed rotor plate (919), the top fixedly connected with of rotor plate (919) rotates even seat (920), the outside of rotating even seat (920) is evenly connected with and places telescopic link (921), the outside of placing telescopic link (921) has been cup jointed and has been placed spring (922), the one end fixedly connected with of placing telescopic link (921) places arc board (923), disinfection fluorescent tube (924) are evenly installed to the inner wall of disinfection top cap (6).
6. The method for preparing high-activity lactic acid bacteria according to claim 5, wherein the driving motor (901) is in transmission connection with the rotating connecting shaft (902) through a gear and a gear, one end of the clamping plate (905) is connected with a corrugated rubber pad, the installation angle of the inclined filter screen (906) is 5-15 degrees, and a sealing ring is installed at the connection position of the detachable cover (910) and the circulating filter bin (909);
the output end of the control switch (4) is electrically connected with the input ends of the driving motor (901), the electric telescopic rod (904), the spray pump (912) and the disinfection lamp tube (924).
7. The preparation method of high activity lactobacillus according to claim 3, wherein the grinding and feeding mechanism (10) is installed on the top end of the feeding cover (7), and the grinding and feeding mechanism (10) comprises a grinding motor (1001), a placing rack (1002), a grinding base (1003), a grinding top seat (1004), a driving gear (1005), a driven gear (1006), a transmission toothed chain (1007), a grinding and feeding bin (1008), a grinding and discharging pipe (1009), a discharging hose (1010), a discharging joint (1011), a feeding electric telescopic rod (1012), a feeding connecting plate (1013), an auxiliary material feeding bin (1014), an auxiliary material feeding pipe (1015) and a grinding guide hole (1016);
the grinding device is characterized in that a grinding motor (1001) is fixedly mounted at the top end of the feeding cover (7), a placing frame (1002) is connected to the position of one side of the grinding motor (1001) corresponding to the top end of the feeding cover (7), a grinding base (1003) is fixedly connected to the position of the lower portion of the placing frame (1002) corresponding to the top end of the feeding cover (7), a grinding top seat (1004) is rotatably mounted at the top end of the grinding base (1003), a driving gear (1005) is fixedly connected to an output shaft of the grinding motor (1001), a driven gear (1006) is fixedly mounted on the outer side of the top end of the grinding top seat (1004), a transmission toothed chain (1007) is mounted on the outer sides of the driving gear (1005) and the driven gear (1006) in a meshed mode, a grinding feeding bin (1008) is fixedly mounted at the top end of the placing frame, the grinding device is characterized in that a discharging hose (1010) is fixedly connected to the bottom end of the grinding discharging pipe (1009), a discharging joint (1011) is fixedly mounted at the bottom end of the discharging hose (1010), feeding electric telescopic rods (1012) are connected to positions, corresponding to the two sides of the grinding discharging pipe (1009), of the bottom end of the feeding cover (7), feeding connecting plates (1013) are connected to the bottom end of each feeding electric telescopic rod (1012), auxiliary material adding bins (1014) are connected to positions, corresponding to the two sides of the grinding base (1003), of the top end of the placing frame (1002), auxiliary material adding pipes (1015) are fixedly mounted at the bottom end of each auxiliary material adding bin (1014), and grinding guide holes (1016) are uniformly formed in the inner wall of the top;
the output end of the control switch (4) is electrically connected with the grinding motor (1001) and the input end of the charging electric telescopic rod (1012).
8. The preparation method of high-activity lactic acid bacteria according to claim 3, wherein a smell removal discharge mechanism (11) is installed inside the smell removal top cover (5), and the smell removal discharge mechanism (11) comprises an air inlet bin (1101), an air inlet duct (1102), an absorption bin (1103), an absorption partition plate (1104), an absorption through hole (1105), an absorption air outlet hole (1106), a smell removal bin (1107), a smell removal air inlet hole (1108), an activated carbon plate (1109), an air exhaust pump (1110) and an air exhaust pipe (1111);
an air inlet bin (1101) is fixedly connected to the inner wall of the bottom end of the odor removal top cover (5), air inlet guide pipes (1102) are connected to two sides of the top end of the air inlet bin (1101), absorbing bins (1103) are fixedly mounted on the inner walls of two ends of the odor removal top cover (5), an absorbing partition plate (1104) is connected to the inner wall of one end of each absorbing bin (1103), absorbing through holes (1105) are uniformly formed in the top end of each absorbing partition plate (1104), and absorbing air outlet holes (1106) are uniformly formed in the position, corresponding to the position above the absorbing partition plate (1104), of one end of each absorbing bin (1103);
the inner walls of two adjacent ends of the odor removal top cover (5) are connected with odor removal bins (1107), odor removal air inlets (1108) are uniformly and symmetrically formed in one ends of the odor removal bins (1107), activated carbon plates (1109) are uniformly connected to positions, corresponding to the odor removal air inlets (1108), of the inner walls of one ends of the odor removal bins (1107), exhaust pumps (1110) are fixedly installed at positions, corresponding to one sides of the activated carbon plates (1109), of the inner walls of one ends of the odor removal bins (1107), and exhaust ends of the exhaust pumps (1110) are connected with exhaust pipes (1111);
the output end of the control switch (4) is electrically connected with the input end of the exhaust pump (1110).
9. The method for preparing high-activity lactobacillus according to claim 3, wherein the inner side of the incubator (2) is provided with a stable shaking mechanism (12) corresponding to the outer side of the culture tank (801), and the stable shaking mechanism (12) comprises a supporting bottom pad (1201), a supporting soft rod (1202), a supporting top pad (1203), a shaking base (1204), a shaking electric telescopic rod (1205), a shaking top base (1206), a stable spring (1207), a stable limiting plate (1208) and a connecting soft belt (1209);
the inner wall of the bottom end of the culture box (2) is uniformly provided with a supporting bottom pad (1201) corresponding to the outer side position of the culture tank (801), the top end of the supporting bottom pad (1201) is fixedly provided with a supporting soft rod (1202), the top end of the supporting soft rod (1202) is fixedly connected with a supporting top pad (1203), the inner wall of the bottom end of the incubator (2) is uniformly connected with a shaking base (1204) at a position corresponding to one side of the supporting bottom pad (1201), the top end of the shaking base (1204) is rotatably provided with a shaking electric telescopic rod (1205), the top end of the shaking electric telescopic rod (1205) is rotatably provided with a shaking top seat (1206), stabilizing springs (1207) are uniformly connected with the outer side of the culture tank (801), a stabilizing limit plate (1208) is connected with the position of the inner wall of the incubator (2) corresponding to the culture tank (801), the inner wall of the stable limit plate (1208) is provided with a connecting soft belt (1209);
the output end of the control switch (4) is electrically connected with the input end of the shaking electric telescopic rod (1205).
10. The method for preparing highly active lactobacillus according to claim 9, wherein the top ends of the rocking top base (1206) and the supporting top pad (1203) are connected to the bottom end of the cultivation tank (801), the other end of the stabilizing spring (1207) is fixedly connected to the inner wall of the cultivation tank (2), and one end of the connecting soft belt (1209) is fixedly connected to the outer side of the cultivation tank (801).
CN202011226633.6A 2020-11-05 2020-11-05 Preparation method of high-activity lactic acid bacteria Withdrawn CN112300966A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606114A (en) * 2022-01-21 2022-06-10 安徽善和生物科技有限公司 Lactic acid bacteria fermentation culture equipment

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114606114A (en) * 2022-01-21 2022-06-10 安徽善和生物科技有限公司 Lactic acid bacteria fermentation culture equipment

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Application publication date: 20210202