CN112294709A - Washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and preparation method thereof - Google Patents

Washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and preparation method thereof Download PDF

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Publication number
CN112294709A
CN112294709A CN202011338709.4A CN202011338709A CN112294709A CN 112294709 A CN112294709 A CN 112294709A CN 202011338709 A CN202011338709 A CN 202011338709A CN 112294709 A CN112294709 A CN 112294709A
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inflammatory
aqueous solution
solution
effects
stirring
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谢峰
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Shanghai Yinghan Import And Export Co ltd
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Shanghai Yinghan Import And Export Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/347—Phenols
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/59—Mixtures
    • A61K2800/592—Mixtures of compounds complementing their respective functions

Abstract

A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects comprises the following components in percentage by mass: 0.01-6% of cannabidiol; 0.1-6% of aqueous solution of ginkgo leaf extract; 0.1-6% of avocado ester aqueous solution; 50-80% of active ingredients; 0.1-2% of a thickening agent; 0.5-5% of a humectant; 0.5-3% of a neutralizer; 0.3-4% of a skin care agent; 0.2-2% of essence; the balance of deionized water. By adding the cannabidiol and the ginkgo leaf extract aqueous solution, the sterilization and bacteriostasis effects of the active ingredients can be more effectively improved, the anti-inflammatory and antioxidant effects are obvious, and the avocado fat can relieve the rough and dry skin caused by the active ingredients, so that the moisturizing and skin-care effects are better.

Description

Washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and preparation method thereof
Technical Field
The invention relates to a washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and a preparation method thereof.
Background
The hand-washing-free disinfectant has the functions of washing hands without water, sterilizing and disinfecting, lasting for a long time, and protecting skin gently, and is commonly used for washing-free hand-washing and disinfecting in the environments without water sources and soaps, such as hospitals, banks, supermarket entertainment venues, restaurants in schools, airports, docks, railway stations, and the like. Hand washing and disinfection is a very important habit in life. Disposable gel disinfection solutions have become one of the necessary disinfection products, both for medical personnel and for family members.
The existing disinfectant has good disinfection and sterilization functions, but as the main components in the disinfectant are chemical components, the disinfectant has certain irritation to the skin of the body surface while being capable of sterilizing, and how to not irritate the skin of the human body while disinfecting, and generates certain anti-inflammatory, moisturizing and anti-oxidation effects, so that the disinfectant is a key research and development direction in the future disinfectant market.
Disclosure of Invention
Aiming at the problems, the invention provides a washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and a preparation method thereof.
The specific technical scheme is as follows:
the washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized by comprising the following components in percentage by mass:
Figure BDA0002798009010000011
further, preferably, the components are composed of the following components in percentage by mass:
Figure BDA0002798009010000012
Figure BDA0002798009010000021
further, the active ingredient is one of ethanol or isopropanol.
Further, the thickening agent is one or more of carbomer, carboxymethyl cellulose, hydroxyethyl cellulose, starch and chitosan, and preferably, the thickening agent is carbomer.
Further, the humectant is one or more of propylene glycol, glycerol, polyethylene glycol and amino acid.
Further, the neutralizing agent is one or two of triethanolamine aqueous solution and sodium hydroxide, preferably, the neutralizing agent is triethanolamine aqueous solution.
Further, the skin care agent is one or more of aloe water solution, coconut oil and vitamin E.
Furthermore, the conductivity of the deionized water is less than 0.5 mu s/cm.
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
e. heating deionized water to 40-50 ℃, sprinkling a thickening agent on the surface, standing and wetting for 3-5 min, stirring until the thickening agent is completely dissolved, then naturally cooling, and cooling to 22-25 ℃ to obtain a solution A;
f. firstly, taking part of ethanol as an active ingredient, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the active ingredient, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
g. adding the residual active ingredients in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; adding humectant, and stirring for 5 min; then adding the solution B, and stirring for 5 min; adding skin care agent, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding a neutralizing agent into the mixture while stirring until a transparent gel is formed;
h. standing and filling.
Compared with the prior art, the invention has the beneficial effects that:
avocado is used as a natural herbal plant, the avocado fat which is an extract of the avocado is a natural oil raw material, the avocado fat takes monounsaturated triglyceride as a main component, and the avocado fat is applied to a wash-free gel disinfectant, can supplement oil which is lacked in epidermal cells of a human body, has excellent skin moistening performance and water locking capacity, can promote cell regeneration and pulse capillary circulation, and has the effects of preventing and delaying skin aging.
The invention adopts the aqueous solution of the ginkgo leaf extract, various trace elements such as flavone, Fe, Cu, Zn, Ma, Me and the like contained in the ginkgo leaf extract form a common body with organic components and the trace elements, and can generate the antibody effect of an activated gene, thereby achieving the effects of fatigue resistance, oxidation resistance and aging resistance.
Cannabidiol (CBD) is used as a non-psychoactive substance in cannabis, and has good anti-inflammatory and antioxidant effects when applied to a wash-free gel disinfectant. Anti-inflammatory effects are primarily associated with their ability to interact with receptors in immune cells. CBD interacts with CB2 receptors, usually located inside immune cells, and by activating these receptors, CBD is able to elicit a variety of immune responses, one of which is anti-inflammatory.
The disinfectant provided by the invention can effectively improve the sterilization and bacteriostasis effects of active ingredients by adding the cannabidiol and the ginkgo leaf extract aqueous solution, has obvious anti-inflammatory and antioxidant effects, and can relieve the rough and dry skin caused by the active ingredients of the skin, thereby having better moisturizing and skin-care effects.
Detailed Description
In order to make the technical solution of the present invention clearer and more clear, the present invention is further described below, and any solution obtained by substituting technical features of the technical solution of the present invention with equivalents and performing conventional reasoning falls within the scope of the present invention.
In the embodiment of the invention, the active ingredients are food-grade raw materials, and the grades of the other components are pharmacopoeia-grade components.
In the embodiment of the invention, the triethanolamine aqueous solution is prepared by mixing triethanolamine: the water-soluble nano-particles are prepared by diluting water in a ratio of 1:9 (volume ratio).
The aloe water solution of the embodiment of the invention is prepared by mixing aloe water solution powder: the water-soluble nano-particles are prepared by diluting with water in a ratio of 1:200 (volume ratio). The aloe aqueous powder was obtained from aloe aqueous powder of south-Henan aloe aqueous bioengineering (USA) Co., Ltd., product type: AA8010 XQ.
The avocado ester aqueous solution of the embodiment of the invention is prepared according to the following steps: the water-soluble nano-particles are prepared by diluting with water in a ratio of 1:200 (volume ratio).
In the embodiment of the invention, the adopted ginkgo biloba extract aqueous solution uses ginkgo biloba extract: water 1:200 (volume ratio), and the ginkgo leaf extract is purchased from Hebeikang pharmaceutical industry Co., Ltd, and has the product name: the ginkgo leaf extract comprises the following effective components (by mass ratio): 24 percent of ginkgetin, 6 percent of ginkgolide and less than 5ppm of ginkgolic acid.
In the embodiment of the invention, the adopted essence is purchased from Robert essence and spice (Beijing) Co., Ltd, and the product types are as follows: 7673 lemon flavor.
In an embodiment of the present invention, cannabidiol in example 1 and example 2 is pure cannabidiol powder; cannabidiol in example 3, example 4 and example 6 cannabidiol oil (with a cannabidiol content of 40% by mass) was used as cannabidiol in example 3, example 4 and example 6, the amount of cannabidiol was based on the actual amount of cannabidiol in the cannabidiol oil; in example 5, cannabidiol was extracted with cannabinol in an amount based on the actual amount of cannabidiol in the cannabinol extract.
Example 1
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: 1.2 percent of cannabidiol; 4% of aqueous solution of ginkgo leaf extract; 4% of avocado fat; 75% of ethanol; 0.35% of carbomer; 1.5% of propylene glycol; 1.2% of glycerol; 1.25% of triethanolamine aqueous solution; aloe water solution 0.5%; 0.2 percent of essence; the balance of deionized water;
b. heating deionized water to 45 ℃, spreading carbomer on the surface, standing and wetting for 4min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 23 ℃ to obtain a solution A;
c. taking ethanol accounting for 40% of the total amount of the substances, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the ethanol, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
d. adding the ethanol which is 35 percent of the total amount of the total substances and remains in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding Aloe water solution, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 20g/s, and adding while stirring until a transparent gel is formed;
e. standing and filling.
Example 2
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: 1.5 percent of cannabidiol; 4% of aqueous solution of ginkgo leaf extract; 4% of avocado fat; 70% of ethanol; 0.3% of carbomer; 0.8 percent of propylene glycol; 0.1% of glycerol; 1% of triethanolamine aqueous solution; 0.5 percent of vitamin E; 0.2 percent of essence; the balance of deionized water;
b. heating deionized water to 48 ℃, spreading carbomer on the surface, standing and wetting for 3min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 24 ℃ to obtain a solution A;
c. firstly, taking ethanol accounting for 50 percent of the total amount of the substances, respectively adding cannabidiol, ginkgo leaf extract aqueous solution and avocado fat into the ethanol, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain solution B;
d. adding the ethanol which is 20 percent of the total amount of the total substances and remains in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding vitamin E, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 20g/s, and adding while stirring until a transparent gel is formed;
e. standing and filling.
Example 3
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: cannabidiol 0.8%; 4% of aqueous solution of ginkgo leaf extract; 4% of avocado fat; 58% of ethanol; 0.25% of carbomer; 0.5 percent of propylene glycol; 0.6 percent of glycerol; 0.9 percent of triethanolamine aqueous solution; aloe water solution 0.5%; 0.2 percent of essence; the balance of deionized water;
b. heating deionized water to 43 ℃, scattering carbomer to the surface, standing and wetting for 4min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 22 ℃ to obtain a solution A;
c. firstly, taking ethanol accounting for 45 percent of the total amount of the total substances, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the ethanol, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
d. adding the ethanol which is 13 percent of the total amount of the total substances and remains in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding Aloe water solution, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 20g/s, and adding while stirring until a transparent gel is formed;
e. standing and filling.
Example 4
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: 6 percent of cannabidiol; aqueous solution of folium Ginkgo extract 6%; 6% of avocado fat; 50% of ethanol; 0.1% of carbomer; 1.5% of propylene glycol; 1.2% of glycerol; 0.5 percent of triethanolamine aqueous solution; 0.3% of aloe aqueous solution; 0.2 percent of essence; the balance of deionized water;
b. heating deionized water to 40 ℃, spreading carbomer on the surface, standing and wetting for 3min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 25 ℃ to obtain a solution A;
c. adding cannabidiol, folium Ginkgo extract water solution, and avocado fat into ethanol respectively, and stirring to dissolve to obtain solution B;
d. adding the ethanol which is 10 percent of the total amount of the total substances and remains in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding Aloe water solution, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 1kg/min, and adding while stirring until a transparent gel is formed;
e. standing and filling.
Example 5
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: cannabidiol 0.1%; 0.1% of aqueous solution of folium Ginkgo extract; avocado butter 0.1%; 80% of ethanol; 2% of carbomer; 0.8 percent of propylene glycol; 0.1% of glycerol; 3% of triethanolamine aqueous solution; vitamin E4%; 2% of essence; the balance of deionized water;
b. heating deionized water to 50 ℃, spreading carbomer on the surface, standing and wetting for 5min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 23 ℃ to obtain a solution A;
c. firstly, taking ethanol accounting for 45 percent of the total amount of the total substances, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the ethanol, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
d. adding the ethanol which is remained in the step b and accounts for 35 percent of the total amount of the total substances into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding Aloe water solution, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 1kg/min, and adding while stirring until a transparent gel is formed;
e. standing and filling.
Example 6
A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. weighing the components in percentage by mass as follows: cannabidiol 0.01%; 80% of ethanol; 2% of carbomer; 2% of propylene glycol; 3% of glycerol; 3% of triethanolamine aqueous solution; vitamin E4%; 2% of essence; the balance of deionized water;
b. heating deionized water to 50 ℃, spreading carbomer on the surface, standing and wetting for 5min, stirring until the carbomer is completely dissolved, then naturally cooling, and cooling to 24 ℃ to obtain a solution A;
c. firstly, taking ethanol accounting for 50 percent of the total amount of the total substances, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the ethanol, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
d. adding the ethanol which is 30 percent of the total amount of the total substances and is remained in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; then adding propylene glycol and glycerol, and continuing stirring for 5 min; then adding the solution B, and stirring for 5 min; adding Aloe water solution, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding triethanolamine aqueous solution at the speed of 1kg/min, and adding while stirring until a transparent gel is formed;
e. standing and filling.
The washing-free gel disinfectant prepared by the invention has excellent sterilization, sensitization prevention, anti-inflammation and oxidation resistance effects.
First, sterilization experiment
The no-clean gel disinfectant prepared by the invention is inspected according to the following criteria: disinfection Specification (2002) microbiological Sterilization test (Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, Escherichia coli)
The test conclusion is as follows:
1. through inspection, the stock solution of the no-clean disinfectant and the D/E neutralizing broth are used as neutralizers, the action of sterilization components in the disinfectant on escherichia coli can be effectively neutralized, the neutralizers and neutralization products do not have adverse effects on tested bacteria and a culture medium, the average sterilization log value of staphylococcus aureus on escherichia coli and staphylococcus aureus is more than 5.00 after 0.5min, 1min and 1.5min, the result meets the standard requirements of Q/321191ACQ 010-2020A and the average sterilization log value of pseudomonas aeruginosa is more than 5.00, and the result meets the standard requirements of the technical disinfection specification (2002).
2. Through inspection, the stock solution of the no-clean disinfectant and the D/E neutralizing broth are used as neutralizers, the action of sterilization components in the disinfectant on Candida albicans can be effectively neutralized, the neutralizers and neutralization products have no adverse effect on tested bacteria and a culture medium, the average sterilization logarithm value of Escherichia coli and Staphylococcus aureus is more than 4.00 after the action of the neutralizers and neutralization products for 0.5min, 1min and 1.5min, and the result meets the requirement of Q/321191ACQ 010-2020 standard.
Second, sensitization test
The no-clean gel disinfectant prepared by the invention is tested by the institute of health and environmental technology of Suzhou university, and is prepared by carrying out sensitization research on white guinea pigs according to the following reference standards: ISO 10093-10; 2010 medical device biological evaluation part 10: irritation and skin sensitization tests; the experimental results show that the skin reaction results after excitation are not obviously changed, the skin sensitization reaction of the experimental samples is not found in the skin of the guinea pig, and the sensitization positive rate is 0%. In conclusion, the invention is mild to skin application and has no sensitization.
Third, anti-oxidation experiment
(1) Superoxide anion scavenging Activity assay
Adding 4mL of TrisHCL (PH8.2) buffer solution machine into the solution with the total volume of 5mL, fixing the volume to 5mL, and placing the solution in a constant-temperature water bath at 25 ℃ for 20 min. Adding 0.2mL of preheated pyrogallol (10mmol/L dissolved in 10mmol/L hydrochloric acid) to initiate reaction, accurately reacting for 5min, adding 60 mu L of 0.1mol/L ascorbic acid aqueous solution to terminate the reaction, and measuring the absorbance A at the wavelength of 420nm within 1h by taking the corresponding ascorbic acid aqueous solution as a blank. The inhibition rate calculation formula is as follows:
Figure BDA0002798009010000071
in the formula: a. the1-absorbance values without sample;
A2-absorbance values free of sample and pyrogallol;
A3-an absorbance value comprising the sample;
A4-absorbance values with sample, but without pyrogallol.
(2) Determination of hydroxyl radical scavenging Activity
Respectively adding 1mL8.8mmol/L FeSO into 5 test tubes41mL of salicylic acid-ethanol solution of 9.1mmol/L, 1mL of the no-clean gel hand sanitizer sample prepared by the invention with different concentrations and 5mL of distilled water, and then 1mL of 0.06% H is added2O2Starting reaction, immediately placing in a 37 deg.C water bath kettle, keeping the temperature for 30min, and measuring absorbance value at 510nm wavelength with distilled water as reference to obtain A1Replace 1mL of H with 1mL of distilled water2O2The absorbance value was measured in the same manner as above and recorded as A2. The absorbance value was measured in the same manner as above using 1mL of distilled water instead of 1mL of the sample and recorded as A3。
Figure BDA0002798009010000081
(3) DPPH free radical scavenging Activity assay
The preparation concentration is 2.0 multiplied by 10-4A solution of DPPH in absolute ethanol in mol/L. Taking 2.0mL of the LDPPH solution, adding samples of the wash-free gel hand sanitizer prepared by the invention with different volumes, adding water to a constant volume of 5mL, standing for 30min, and measuring the absorbance.
Figure BDA0002798009010000082
In the formula: a. the1The absorbance when the volume of the 2.0mL LDPPH solution is 5 mL;
Ai-after 2.0ml of PPH solution is added to the sample, the sample is fixedAbsorbance at 5 mL.
The inhibition rates of the three are shown in the following table:
superoxide anion Hydroxyl radical DPPH free radical
Inhibition rate IC50 7.34ug/ml 6.89ug/ml 0.356ug/ml
Experiments show that the disinfection gel containing CBD has a certain antioxidant effect.
Fourth, anti-inflammatory Performance test
(1) The samples of the no-clean gel hand sanitizer prepared by the invention are adopted in small dose (smearing once, 3ml each time), medium dose (smearing twice, 3ml each time) and large dose (smearing three times, 3ml each time).
(2) Flufenamic acid butyl ester ointment (applied once, 3ml each time) (trade name of Bunte, manufactured by Hippon antibiotic factory, Cogeneration group, national drug standard number: H20030188) as a control group;
(3) 50 male mice of 25-30g are randomly divided into a blank group, a large dose group, a medium dose group, a small dose group and a control group according to the body weight, and 10 mice are taken in each group. On the same day of experiment, the two sides of ears of each group of mice are coated with corresponding medicines, the area is about 3cm multiplied by 1cm, after 45min, each group is scrubbed by a distilled water cotton ball to remove the liquid medicine, the liquid medicine is scrubbed by a dry cotton ball, 0.05 mL/mouse of dimethylbenzene is coated on the two sides of the right ear of the mouse to cause swelling, and the left ear is used as a control. After 30min, the neck of the mouse was sacrificed, round ear pieces were punched out of the same part of both ears of each animal using a 8mm diameter punch, the round ear pieces were placed on an electronic analytical balance and weighed, and the difference between the weights of the left and right ear pieces was used as the swelling degree to determine the swelling inhibition rate.
Figure 1
Specific inhibition data are shown in the following table:
group of Swelling degree (m/mg) Swelling inhibition ratio (%)
Blank space 3.68±0.98 /
Small dose 3.37±0.96 8.16
Middle dose 3.18±0.93 13.59
High dose 2.96±0.91 19.57
Positive forControl 2.35±0.66 36.14
Experiments show that the disinfection gel containing CBD has certain anti-inflammatory effect.

Claims (10)

1. The washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized by comprising the following components in percentage by mass:
Figure FDA0002798008000000011
2. the leave-in gel disinfectant with anti-inflammatory and anti-oxidant effects as claimed in claim 1, further comprising an aqueous solution of ginkgo biloba extract.
3. The leave-on gel disinfectant with anti-inflammatory and antioxidant effects as claimed in claim 1, further comprising an aqueous solution of shea butter.
4. The leave-on gel disinfectant with anti-inflammatory and anti-oxidant effects as claimed in claim 1, wherein said active ingredient is one of ethanol or isopropanol.
5. The leave-on gel disinfectant with anti-inflammatory and antioxidant effects as claimed in claim 1, wherein said thickener is one or more of carbomer, carboxymethyl cellulose, hydroxyethyl cellulose, starch, and chitosan.
6. The leave-on gel disinfectant with anti-inflammatory and anti-oxidant effects as claimed in claim 1, wherein said humectant is one or more of propylene glycol, glycerol, polyethylene glycol, and amino acids.
7. The no-clean gel disinfectant with anti-inflammatory and anti-oxidant effects as claimed in claim 1, wherein said neutralizer is one or two of triethanolamine and sodium hydroxide, preferably said neutralizer is triethanolamine.
8. The leave-on gel disinfectant with anti-inflammatory and antioxidant effects as claimed in claim 1, wherein the skin care agent is one or more of aloe vera aqueous solution, coconut oil, and vitamin E.
9. The disposable gel disinfectant of claim 1 wherein the deionized water has a conductivity of less than 0.5 μ s/cm.
10. A washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects is characterized in that the preparation method comprises the following steps:
a. heating deionized water to 40-50 ℃, sprinkling a thickening agent on the surface, standing and wetting for 3-5 min, stirring until the thickening agent is completely dissolved, then naturally cooling, and cooling to 22-25 ℃ to obtain a solution A;
b. firstly, taking part of active ingredients, respectively adding cannabidiol, a ginkgo leaf extract aqueous solution and avocado fat into the active ingredients, and fully stirring until the cannabidiol, the ginkgo leaf extract aqueous solution and the avocado fat are dissolved to obtain a solution B;
c. adding the residual active ingredients in the step b into the solution A, and keeping the low temperature and fully stirring for 10 min; adding humectant, and stirring for 5 min; then adding the solution B, and stirring for 5 min; adding skin care agent, and stirring for 5 min; adding essence again, and stirring for 5 min; finally, slowly adding a neutralizing agent into the mixture while stirring until a transparent gel is formed;
d. standing and filling.
CN202011338709.4A 2020-11-25 2020-11-25 Washing-free gel disinfectant with anti-inflammatory and anti-oxidation effects and preparation method thereof Pending CN112294709A (en)

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Application publication date: 20210202