CN112266825A - Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics - Google Patents
Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics Download PDFInfo
- Publication number
- CN112266825A CN112266825A CN202011182423.1A CN202011182423A CN112266825A CN 112266825 A CN112266825 A CN 112266825A CN 202011182423 A CN202011182423 A CN 202011182423A CN 112266825 A CN112266825 A CN 112266825A
- Authority
- CN
- China
- Prior art keywords
- oil
- essential oil
- seed oil
- compound essential
- nanocapsule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000341 volatile oil Substances 0.000 title claims abstract description 145
- 150000001875 compounds Chemical class 0.000 title claims abstract description 92
- 230000002087 whitening effect Effects 0.000 title claims abstract description 62
- 230000001153 anti-wrinkle effect Effects 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 239000002537 cosmetic Substances 0.000 title claims abstract description 34
- 235000015112 vegetable and seed oil Nutrition 0.000 claims abstract description 121
- 239000002088 nanocapsule Substances 0.000 claims abstract description 54
- 235000020971 citrus fruits Nutrition 0.000 claims abstract description 42
- 241000207199 Citrus Species 0.000 claims abstract description 41
- 241000586290 Suaeda salsa Species 0.000 claims abstract description 34
- 240000000233 Melia azedarach Species 0.000 claims abstract description 32
- 239000003921 oil Substances 0.000 claims abstract description 27
- 235000019198 oils Nutrition 0.000 claims abstract description 27
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 19
- 239000006002 Pepper Substances 0.000 claims abstract description 19
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 19
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 19
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 19
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000004945 emulsification Methods 0.000 claims abstract description 6
- 230000002441 reversible effect Effects 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims description 47
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 241000722363 Piper Species 0.000 claims description 18
- 239000006071 cream Substances 0.000 claims description 16
- 238000007873 sieving Methods 0.000 claims description 16
- 235000007650 Aralia spinosa Nutrition 0.000 claims description 14
- 241000949456 Zanthoxylum Species 0.000 claims description 14
- 238000010298 pulverizing process Methods 0.000 claims description 12
- 238000002791 soaking Methods 0.000 claims description 12
- 230000003020 moisturizing effect Effects 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 10
- 235000010413 sodium alginate Nutrition 0.000 claims description 10
- 239000000661 sodium alginate Substances 0.000 claims description 10
- 229940005550 sodium alginate Drugs 0.000 claims description 10
- 239000006210 lotion Substances 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 8
- 239000008267 milk Substances 0.000 claims description 8
- 210000004080 milk Anatomy 0.000 claims description 8
- 235000013336 milk Nutrition 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 7
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 4
- 230000005855 radiation Effects 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 238000001256 steam distillation Methods 0.000 claims description 4
- 244000089698 Zanthoxylum simulans Species 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 239000002552 dosage form Substances 0.000 claims description 2
- 238000003760 magnetic stirring Methods 0.000 claims description 2
- 238000005406 washing Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 27
- 102000003425 Tyrosinase Human genes 0.000 abstract description 17
- 108060008724 Tyrosinase Proteins 0.000 abstract description 17
- 239000004480 active ingredient Substances 0.000 abstract description 5
- 231100000331 toxic Toxicity 0.000 abstract description 4
- 230000002588 toxic effect Effects 0.000 abstract description 4
- 239000000419 plant extract Substances 0.000 abstract description 3
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 2
- 244000203593 Piper nigrum Species 0.000 abstract 1
- 230000009758 senescence Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 108
- 238000012360 testing method Methods 0.000 description 24
- 239000000243 solution Substances 0.000 description 20
- 241000196324 Embryophyta Species 0.000 description 16
- 238000002835 absorbance Methods 0.000 description 16
- 230000002292 Radical scavenging effect Effects 0.000 description 15
- 239000000463 material Substances 0.000 description 15
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 12
- 230000037303 wrinkles Effects 0.000 description 12
- 241000918237 Suaeda glauca Species 0.000 description 11
- 238000002156 mixing Methods 0.000 description 11
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 10
- TUJKJAMUKRIRHC-UHFFFAOYSA-N hydroxyl Chemical compound [OH] TUJKJAMUKRIRHC-UHFFFAOYSA-N 0.000 description 10
- 239000001301 oxygen Substances 0.000 description 10
- 229910052760 oxygen Inorganic materials 0.000 description 10
- 230000001965 increasing effect Effects 0.000 description 9
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 8
- 230000007774 longterm Effects 0.000 description 8
- 238000005259 measurement Methods 0.000 description 8
- 239000013641 positive control Substances 0.000 description 8
- 230000002829 reductive effect Effects 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 7
- 230000003647 oxidation Effects 0.000 description 7
- 238000007254 oxidation reaction Methods 0.000 description 7
- 230000001603 reducing effect Effects 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 230000036564 melanin content Effects 0.000 description 6
- 229940079877 pyrogallol Drugs 0.000 description 6
- 150000003254 radicals Chemical class 0.000 description 6
- 241000282414 Homo sapiens Species 0.000 description 5
- 229960000271 arbutin Drugs 0.000 description 5
- 230000007547 defect Effects 0.000 description 5
- -1 hydroxyl radicals Chemical class 0.000 description 5
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 5
- 230000037394 skin elasticity Effects 0.000 description 5
- 238000010998 test method Methods 0.000 description 5
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 4
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 4
- 230000032683 aging Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 230000002401 inhibitory effect Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000019483 Peanut oil Nutrition 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000007760 free radical scavenging Effects 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000000312 peanut oil Substances 0.000 description 3
- 239000010453 quartz Substances 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- 208000004998 Abdominal Pain Diseases 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 208000003351 Melanosis Diseases 0.000 description 2
- 230000010757 Reduction Activity Effects 0.000 description 2
- 241001093501 Rutaceae Species 0.000 description 2
- 229920002385 Sodium hyaluronate Polymers 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 229960005070 ascorbic acid Drugs 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 229940075507 glyceryl monostearate Drugs 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000008099 melanin synthesis Effects 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 239000001788 mono and diglycerides of fatty acids Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000008055 phosphate buffer solution Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 229940010747 sodium hyaluronate Drugs 0.000 description 2
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 2
- 239000012085 test solution Substances 0.000 description 2
- LQIAZOCLNBBZQK-UHFFFAOYSA-N 1-(1,2-Diphosphanylethyl)pyrrolidin-2-one Chemical compound PCC(P)N1CCCC1=O LQIAZOCLNBBZQK-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 102000030523 Catechol oxidase Human genes 0.000 description 1
- 108010031396 Catechol oxidase Proteins 0.000 description 1
- 241000871189 Chenopodiaceae Species 0.000 description 1
- 206010008570 Chloasma Diseases 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 1
- 201000004624 Dermatitis Diseases 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 206010014970 Ephelides Diseases 0.000 description 1
- 206010049077 Hernia pain Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 241000158728 Meliaceae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000242678 Schistosoma Species 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003266 anti-allergic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 201000009361 ascariasis Diseases 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229960001631 carbomer Drugs 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 231100000135 cytotoxicity Toxicity 0.000 description 1
- 230000003013 cytotoxicity Effects 0.000 description 1
- ZAKOWWREFLAJOT-UHFFFAOYSA-N d-alpha-Tocopheryl acetate Natural products CC(=O)OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-UHFFFAOYSA-N 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 1
- 229960004337 hydroquinone Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 230000005808 skin problem Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 229940084106 spermaceti Drugs 0.000 description 1
- 239000012177 spermaceti Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229940042585 tocopherol acetate Drugs 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- General Chemical & Material Sciences (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to compound essential oil, a preparation method thereof and application thereof in whitening and anti-wrinkle cosmetics. The compound essential oil is prepared from suaeda salsa seed oil, pepper seed oil, citrus seed oil and szechwan chinaberry fruit oil according to the weight ratio of 3-4: 1-2: 2-3: 5-6, preparing the nanocapsule containing 33% of compound essential oil according to a reverse emulsification method, and adding the nanocapsule into the whitening anti-wrinkle cosmetic according to the proportion of 10-15%. The compound essential oil is a pure natural plant extract, can inhibit tyrosinase activity, is efficient in antioxidation, can effectively reduce volatilization of the essential oil by preparing the compound essential oil into a nanocapsule, can protect active ingredients of the essential oil, is added into cosmetics, is mild and has no toxic or side effect, and can whiten skin and delay senescence after being used for a long time.
Description
Technical Field
The invention relates to compound essential oil, in particular to compound essential oil consisting of suaeda salsa seed oil, pepper seed oil, citrus seed oil and szechwan chinaberry fruit oil, a preparation method of the compound essential oil and application of the compound essential oil in daily cosmetics for whitening and resisting wrinkles.
Background
In the modern society, the living standard and aesthetic standard of people are continuously improved, and people have fair and smooth skin which is advocated by modern women. In recent years, the development of whitening anti-wrinkle cosmetics is becoming active, and in view of the current development situation of the global cosmetics industry, natural active ingredients are obtained from Chinese herbal medicines, so that the natural active ingredients are becoming a new popular trend for the development of the cosmetics industry.
Tyrosinase, also known as polyphenol oxidase, is a copper-containing oxidoreductase of about 75kb, widely present in animals, plants, microorganisms and human bodies, and is a rate-limiting enzyme for melanin synthesis, which directly affects the synthesis of melanin. Meanwhile, it is also related to the occurrence of diseases such as excessive melanin deposition such as freckles, chloasma and the like. Therefore, the real whitening of the skin can be realized by inhibiting the activity of tyrosinase. At present, skin whitening active substances on the market are mainly tyrosinase inhibitors, including arbutin, kojic acid, hydroquinone and the like, but the skin whitening active substances have some defects, such as unstable properties, easy oxidative discoloration, weak efficacy caused by poor percutaneous absorption, certain cytotoxicity, easy skin irritation and the like. Therefore, the development of safer and more effective whitening products is a hot point of research in the field of cosmetics.
Aging is an "oxidation process" of the human body. In the skin, oxidation is usually an influence of radicals. After the skin is oxidized, the human body can generate redundant free radicals. Our usual anti-aging care, namely "anti-oxidation", is to reduce free radicals in the skin mainly by some methods. Therefore, the antioxidant belongs to basic care of skin, is indispensable care, is equivalent to a skin's dart', provides certain protective effect to skin, prevents color cosmetics and ultraviolet rays from influencing the skin, and can delay aging, enable the skin to be in a relatively tight state and prevent wrinkles from generating.
The plant essential oil is a pure natural plant extract, can relieve various skin problems after long-term facial use, can reduce wrinkles, delay skin aging, soften skin, regulate oil secretion of skin, improve the water deficiency state of skin, enable the skin to have luster, sterilize, diminish inflammation, relieve skin and the like; the plant essential oil is a pure natural substance, does not contain other chemical additives such as preservatives and the like, is easy to be absorbed and transformed by the skin, and cannot be deposited on the surface of the skin.
Suaeda glauca is a herbaceous plant of Chenopodiaceae and is one of indication plants of saline-alkali soil. The suaeda salsa is an oil plant with potential application value and mainly grows in saline-alkali wastelands such as seashore, desert and the like and tidal flat lands. The suaeda glauca seeds contain a large amount of fat, the extracted essential oil has extremely high nutritional value, and can reduce blood fat, and the suaeda glauca seeds can also be used as natural edible pigment to be added into feed.
The pepper is a rutaceae plant and is widely distributed in China. The prickly ash seeds contain a large amount of aromatic components and essential oil, and are one of eight traditional seasonings in China. The Chinese prickly ash is used as a traditional Chinese medicine and has the effects of warming the middle-jiao and promoting the circulation of qi, expelling cold, relieving pain and killing insects; treating stomach and abdomen psychroalgia, emesis, diarrhea, schistosome, ascariasis, etc.; can also be used as epidermal anesthetic.
Citrus is a plant of the genus Citrus in the family Rutaceae, China is a world large producing country of citrus, and planting area and yield are the first place in the world. The citrus fruits contain more than 30 health-care substances, have high nutritional value, and can resist tumor, soothe the liver, strengthen the spleen, resolve phlegm, regulate qi and the like; the citrus seed extract has antibacterial effect.
Fructus Toosendan is a dried mature fruit of Meliaceae plant, and is a well-known Chinese medicinal material. The product contains a large amount of volatile oil substances, has high medicinal value, and can be used for treating stagnation of liver-qi, pathogenic fire, chest and hypochondrium, abdominal pain, hernia pain, and abdominal pain due to parasitic infestation.
The plant essential oil has various extraction methods, and different extraction methods can cause the difference of the components, the content, the biological activity and the like of the plant essential oil. The invention also discovers that the compound essential oil has tyrosinase activity inhibiting and antioxidant activities and can be used in whitening cosmetics. However, the plant essential oil as a fat-soluble liquid has volatility, and the storage condition of the essential oil is very harsh, so that the storage condition of the essential oil is limited in practical application; can protect the active components of the essential oil; the stability of the essential oil can be improved; the storage period of the essential oil can be prolonged; can broaden the application range of the essential oil. The compound essential oil nanocapsule can be added into daily cosmetics such as water, milk, face cream, etc.
The inventor finally obtains the compound essential oil with the optimal proportion through a large number of research experiments, and proves the positive effects of the compound essential oil on whitening and wrinkle resistance through rigorous experiments. The invention provides compound essential oil and a nanocapsule prepared from the compound essential oil, which can play a role in whitening and resisting wrinkles when added into cosmetics.
Disclosure of Invention
The first purpose of the invention is to provide the compound essential oil which is simple in extraction method and has the effects of whitening and resisting wrinkles.
The second purpose of the invention is to provide a preparation method of the compound essential oil and the nanocapsules thereof.
The third purpose of the invention is to provide the application of the compound essential oil nanocapsule in the preparation of the whitening anti-wrinkle daily-use cosmetic.
The invention provides compound essential oil which is prepared from suaeda salsa seed oil, pepper seed oil, citrus seed oil and szechwan chinaberry fruit oil in a proportion of 3-4: 1-2: 2-3: 5-6.
The compound essential oil comprises the following components in a ratio of 3:1:2: 5.
Preferably, the preparation method of the suaeda salsa seed oil in the compound essential oil comprises the following steps:
taking suaeda salsa seeds, crushing, sieving, placing in a charging basket of microwave dynamic extraction equipment, adding 60 times of water, soaking for 45min at room temperature, setting the microwave power to 287W, performing microwave radiation for 30min, connecting a volatile oil separator on the microwave extraction equipment, and collecting distilled essential oil to obtain the suaeda salsa seed oil.
The preparation method of the pricklyash seed oil in the compound essential oil comprises the following steps:
pulverizing semen Zanthoxyli, sieving, and placing in supercritical CO2Adding 70% ethanol in an amount which is 2.5 times that of the extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 40MPa, the extraction temperature is 40 ℃, the extraction time is 3h, and CO is added2The flow rate was 20 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain oleum Zanthoxyli Bungeani.
The preparation method of the citrus seed oil in the compound essential oil comprises the following steps:
pulverizing citrus seeds, sieving, and placing in supercritical CO2Adding 3 times of 70% ethanol into an extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 35MPa, the extraction temperature is 45 ℃, the extraction time is 3h, and CO is2The flow rate was 25 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain citrus seed oil.
The preparation method of the chinaberry fruit oil in the compound essential oil comprises the following steps:
pulverizing fructus Toosendan, sieving, placing in round bottom flask, adding 10 times of distilled water, adjusting pH to 4.5 with hydrochloric acid, adding 1% cellulase into container, slightly stirring, placing in water bath, performing enzymolysis at 50 deg.C for 35min, performing steam distillation, installing volatile oil extractor, heating to boil, distilling for 2.5 hr, and collecting distilled essential oil to obtain fructus Toosendan oil.
The invention also provides a preferable preparation method of the compound essential oil, which comprises the following steps:
(1) preparation of suaeda salsa seed oil
Taking suaeda salsa seeds, crushing, sieving, placing in a charging basket of microwave dynamic extraction equipment, adding 60 times of water, soaking for 45min at room temperature, setting the microwave power to 287W, performing microwave radiation for 30min, connecting a volatile oil separator on the microwave extraction equipment, and collecting distilled essential oil to obtain the suaeda salsa seed oil.
(2) Preparation of pricklyash seed oil
Pulverizing semen Zanthoxyli, sieving, and placing in supercritical CO2Adding 70% ethanol 2.5 times the amount of the extract in an extraction kettle, and soaking at room temperature150min, and the extraction conditions are as follows: the extraction pressure is 40MPa, the extraction temperature is 40 ℃, the extraction time is 3h, and CO is added2The flow rate was 20 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain oleum Zanthoxyli Bungeani.
(3) Preparation of citrus seed oil
Pulverizing citrus seeds, sieving, and placing in supercritical CO2Adding 3 times of 70% ethanol into an extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 35MPa, the extraction temperature is 45 ℃, the extraction time is 3h, and CO is2The flow rate was 25 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain citrus seed oil.
(4) Preparation of Chuanlian chinaberry seed oil
Pulverizing fructus Toosendan, sieving, placing in round bottom flask, adding 10 times of distilled water, adjusting pH to 4.5 with hydrochloric acid, adding 1% cellulase into container, slightly stirring, placing in water bath, performing enzymolysis at 50 deg.C for 35min, performing steam distillation, installing volatile oil extractor, heating to boil, distilling for 2.5 hr, and collecting distilled essential oil to obtain fructus Toosendan oil.
(5) Selecting Suaeda salsa seed oil, pricklyash peel seed oil, citrus seed oil and fructus Toosendan oil according to a certain proportion, and mixing uniformly to obtain the final product.
The compound essential oil for the whitening anti-wrinkle cosmetic is prepared into nanocapsules containing 33% of compound essential oil according to a reverse emulsification method, and the nanocapsules are added into the whitening anti-wrinkle cosmetic according to the proportion of 10-15%.
The whitening anti-wrinkle cosmetic has no special limitation on the selection of the dosage form and the matrix, and can be prepared into whitening anti-wrinkle water, whitening anti-wrinkle milk, whitening anti-wrinkle cream and the like.
The invention also comprises the application of the compound essential oil in the field of whitening and anti-wrinkle cosmetics.
The compound essential oil has the following beneficial effects in the field of whitening and anti-wrinkle cosmetics:
1. the compound essential oil is a pure natural plant extract, has wide raw material sources, no toxic or side effect after long-term use, simple preparation process and enriched effective active ingredients, and is suitable for long-term whitening and wrinkle resistance.
2. The invention originally combines the suaeda salsa seed oil, the pepper seed oil, the citrus seed oil and the szechwan chinaberry fruit oil, and determines the dosage and proportion of the components through a large number of experiments, so that the compound essential oil has obvious synergistic effect. By optimizing the preparation scheme and adopting a reverse emulsification method, the compound essential oil nanocapsule is prepared, so that the volatilization loss of essential oil can be reduced, the active ingredients of the essential oil are protected, and the storage time of the essential oil is prolonged. The compound essential oil nanocapsule is added into cosmetics, is mild and has no toxic or side effect, and can remarkably inhibit tyrosinase activity, remove free radicals, resist oxidation, whiten skin and delay aging after long-term use.
Detailed Description
The technical solution of the present invention will be further described with reference to the following specific examples, but the embodiments of the present invention are not limited thereto.
Example 1 preparation of skin whitening anti-wrinkle series cosmetics
The first step is as follows: preparation of various essential oils
The preparation method of the suaeda salsa seed oil comprises the following steps:
taking suaeda salsa seeds, crushing, sieving, placing in a charging basket of microwave dynamic extraction equipment, adding 60 times of water, soaking for 45min at room temperature, adding 0.1% of cellulase, setting the microwave power to 287W, performing microwave radiation for 30min, connecting a volatile oil separator on the microwave extraction equipment, and collecting distilled essential oil to obtain the suaeda salsa seed oil.
The preparation method of the pricklyash seed oil comprises the following steps:
pulverizing semen Zanthoxyli, sieving, and placing in supercritical CO2Adding 70% ethanol in an amount which is 2.5 times that of the extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 40MPa, the extraction temperature is 40 ℃, the extraction time is 3h, and CO is added2The flow rate was 20 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain oleum Zanthoxyli Bungeani.
The preparation method of the citrus seed oil comprises the following steps:
pulverizing citrus seeds, sieving, and placing in supercritical CO2Adding 70% ethanol 3 times the amount of the extract in an extraction kettle, and soaking at room temperature150min, and the extraction conditions are as follows: the extraction pressure is 35MPa, the extraction temperature is 45 ℃, the extraction time is 3h, and CO is2The flow rate was 25 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain citrus seed oil.
The preparation method of the szechwan chinaberry fruit oil comprises the following steps:
pulverizing fructus Toosendan, sieving, placing in round bottom flask, adding 10 times of distilled water, adjusting pH to 4.5 with hydrochloric acid, adding 1% cellulase into container, slightly stirring, placing in water bath, performing enzymolysis at 50 deg.C for 35min, performing steam distillation, installing volatile oil extractor, heating to boil, distilling for 2.5 hr, and collecting distilled essential oil to obtain fructus Toosendan oil.
The second step is that: preparing a compound essential oil nanocapsule:
taking 0.1g of acetyl chitosan, placing the acetyl chitosan in a beaker, and adding deionized water to 10mL to obtain an acetyl chitosan solution for later use; taking 10g of sodium alginate, placing the sodium alginate in a beaker, adding 10mL of compound essential oil, carrying out ultrasonic emulsification completely, slowly dropwise adding 0.5mol/LNaOH to adjust the pH value to 7.5 to obtain sodium alginate nanocapsules, washing and centrifuging the sodium alginate nanocapsules, adding the sodium alginate nanocapsules into an acetyl chitosan solution, and carrying out magnetic stirring for 3 hours to obtain the compound essential oil nanocapsules.
The third step: the compound essential oil nanocapsule is added into daily cosmetics to prepare whitening anti-wrinkle moisturizing water, whitening anti-wrinkle milk and whitening anti-wrinkle cream.
The invention provides an application of the compound essential oil nanocapsule in a whitening and anti-wrinkle cosmetic, and preferably the compound essential oil nanocapsule is added in the whitening and anti-wrinkle cosmetic in an amount of 10-15% by mass.
The whitening anti-wrinkle cosmetic has no special limitation on the selection of the formulation and the matrix, and can be prepared into whitening anti-wrinkle moisturizing lotion, whitening anti-wrinkle milk, whitening anti-wrinkle cream and the like.
Preparing whitening anti-wrinkle moisturizing lotion:
the components and contents of the whitening anti-wrinkle moisturizing lotion are shown in table 1.
TABLE 1 whitening anti-wrinkle moisturizing lotion formula
The preparation process comprises the following steps:
adding the compound essential oil nanocapsule into sodium hyaluronate, and uniformly mixing to obtain a mixed material A; and adding the mixed material A and the other components into deionized water, and uniformly mixing to obtain the whitening anti-wrinkle moisturizing lotion.
Preparation of the whitening anti-wrinkle milk:
the components and contents of the whitening anti-wrinkle milk are shown in table 2.
TABLE 2 whitening anti-wrinkle emulsion formula
The preparation process comprises the following steps:
heating vitamin E acetate, methyl paraben, polyethylene glycol (25) spermaceti stearate, glyceryl monostearate, and dimethyl silicone oil as oil phase to 50 deg.C, stirring, and keeping the temperature for 25 min; heating glycerol, carbomer and deionized water as water phase to 50 deg.C, stirring, and keeping the temperature for 25 min; adding the oil phase into an emulsifying pot, maintaining the temperature at 50 deg.C, starting homogenizing and stirring at the stirring speed of 27r/min, pumping the water phase into the emulsifying pot, homogenizing for 5min, and stirring for 20 min; cooling to 35 ℃, adding the compound essential oil nanocapsules, stirring and mixing uniformly, and cooling to room temperature to obtain the whitening anti-wrinkle emulsion.
Preparation of whitening anti-wrinkle cream:
the components and contents of the whitening anti-wrinkle cream are shown in table 3.
TABLE 3 whitening anti-wrinkle cream formula
The preparation process comprises the following steps:
uniformly mixing sodium hyaluronate, glyceryl monostearate and compound essential oil nanocapsules to obtain a mixed material A; placing medical vaseline and glycerol on a water bath kettle, heating to 75 ℃ and dissolving to obtain a mixed material B; and when the temperature of the mixed material B is reduced to 25 ℃, adding the hexadecanol, the propyl p-hydroxybenzoate, the polyacrylamide and the mixed material A, uniformly stirring, adding deionized water, and adjusting the pH value to 5.5-6 to obtain the whitening anti-wrinkle cream.
The following test examples further illustrate that the compound essential oil has the effects of inhibiting tyrosinase activity, removing hydroxyl free radicals and oxygen free radicals and reducing force, can be used for preparing series of cosmetics for whitening and resisting wrinkles, and the trial effect of the series of cosmetics on human bodies.
Test example 1
The tyrosinase inhibition test of the compound essential oil comprises the following steps:
1. materials and methods
1.1 test materials
Suaeda salsa seed oil, pepper seed oil, citrus seed oil and Chinaberry fruit oil are respectively prepared according to the embodiment. Preparing compound essential oil (composition 1, lacking Chinaberry fruit oil) of Suaeda glauca seed oil, pricklyash peel seed oil and citrus seed oil according to the formula proportion; compound essential oil of suaeda salsa seed oil, pepper seed oil and chinaberry seed oil (composition 2, lacking of citrus seed oil); compound essential oil of suaeda glauca seed oil, citrus seed oil and chinaberry seed oil (composition 3, lacking of pepper seed oil); compound essential oil of pricklyash peel seed oil, orange seed oil and chinaberry seed oil (composition 4, lacking suaeda salsa seed oil); the essential oil missing in the composition is replaced by peanut oil with equal proportion. The invention is compounded with essential oil (composition 5); the compound essential oil nanocapsule (composition 6, nanocapsule prepared by composition 5); positive control arbutin.
1.2 test methods
The inhibition effect of samples on tyrosinase activity is measured by a dopa rate oxidation method, samples are added according to table 4, phosphate buffer solution, the samples and tyrosinase are sequentially added into a test tube, water bath is carried out at 35 ℃ for 15min, then tyrosine is added, the mixture is uniformly mixed, reaction is carried out at 35 ℃ for 30min, the mixture is rapidly transferred into a cuvette, and the absorbance value is measured at 475 nm. Each group was assayed 3 times in parallel with arbutin as a positive control.
Calculating the formula: tyrosinase inhibition rate [ (a-B)/a ] x 100%;
wherein A is the absorbance value of the standard control, and B is the absorbance value of the test solution or the positive control.
TABLE 4 reaction solution composition
Note: when the absorbance is measured by a spectrophotometer, the test solution, the standard control and the positive control are respectively zeroed by the negative control 1, the negative control 2 and the negative control 3.
2. Results and analysis
The inhibition of tyrosinase by each composition is shown in table 5.
TABLE 5 tyrosinase inhibition test results for each composition
In comparison with the composition 1, the composition,△P<0.01; in comparison with the composition 2, the present inventors have shown that,▲P<0.01; in comparison with the composition 3, the composition,＊P<0.01; in comparison with the composition 4, the composition,θP<0.01; in comparison with the composition No. 5,×P<0.01; compared with the arbutin, the preparation method has the advantages that,#P<0.01。
as shown in Table 5, each composition can inhibit tyrosinase activity and has whitening effect, wherein the tyrosinase inhibition rate of the composition 6 is the highest, and the whitening activity of the composition is higher than that of arbutin. Compared with the compositions 1, 2, 3 and 4, the whitening effect of the composition 5 is obviously improved, which shows that the whitening effect is improved after the combination of the 4 natural plant essential oils, and the effect is reduced if any one of the natural plant essential oils is absent. The composition 5 can be used for preparing nanocapsules, which have tyrosinase inhibition rate significantly higher than that of composition 5, because essential oils in compositions 1-5 are volatile and have short action time, and the nanocapsules prepared by the method can overcome the defect and improve anti-allergy activity.
3. And (4) conclusion: the compound essential oil and the nanocapsule thereof can effectively inhibit the activity of tyrosinase, thereby achieving the effect of whitening.
Test example 2
The hydroxyl radical scavenging activity test of the compound essential oil of the invention comprises:
1. materials and methods
1.1 test materials
Suaeda salsa seed oil, pepper seed oil, citrus seed oil and Chinaberry fruit oil are respectively prepared according to the embodiment. Preparing compound essential oil (composition 1, lacking Chinaberry fruit oil) of Suaeda glauca seed oil, pricklyash peel seed oil and citrus seed oil according to the formula proportion; compound essential oil of suaeda salsa seed oil, pepper seed oil and chinaberry seed oil (composition 2, lacking of citrus seed oil); compound essential oil of suaeda glauca seed oil, citrus seed oil and chinaberry seed oil (composition 3, lacking of pepper seed oil); compound essential oil of pricklyash peel seed oil, orange seed oil and chinaberry seed oil (composition 4, lacking suaeda salsa seed oil); the essential oil missing in the composition is replaced by peanut oil with equal proportion. The invention is compounded with essential oil (composition 5); the compound essential oil nanocapsule (composition 6, nanocapsule prepared from composition 5); positive control vitamin c (vc) in water.
1.2 test methods
Measuring hydroxyl radical scavenging activity by Fenton method, adding sample and ferrous sulfate (FeSO) into test tube according to Table 64) Mixing with salicylic acid, standing at room temperature for 15min, adding hydrogen peroxide (H) into the test tube2O2) Standing at room temperature for 35min after mixing uniformly, and finally measuring the absorbance value at the wavelength of 510nm by using an enzyme-labeling instrument, wherein each group is subjected to parallel measurement for 3 times, and VC is used as a positive control.
Calculating the formula: hydroxyl radical scavenging rate Ao- (Ax-Axo)/Ao × 100%;
wherein Ao is the absorbance value of the blank control, Ax is the absorbance value of the added sample, and Axo is the absorbance value of the added sample without H2O2Aox is the absorbance value of the positive control.
TABLE 6 reaction solution composition
2. Results and analysis
The hydroxyl radical scavenging rate of each composition is shown in table 7.
TABLE 7 hydroxy radical scavenging test results for each composition
In comparison with the composition 1, the composition,△P<0.05，△△P<0.01; in comparison with the composition 2, the present inventors have shown that,▲P<0.01; in comparison with the composition 3, the composition,＊＊P<0.05，＊P<0.01; in comparison with the composition 4, the composition,θP<0.01; in comparison with the composition No. 5,×P<0.05; in comparison with VC, the comparison is made,#P<0.01。
as can be seen from table 7, each composition had hydroxyl radical scavenging activity, and composition 6 had the highest hydroxyl radical scavenging rate, which was higher than VC powder. Compared with the compositions 1, 2, 3 and 4 respectively, the hydroxyl radical scavenging rate of the composition 5 is obviously improved, which shows that the activity of scavenging hydroxyl radicals is improved after the combination of the 4 natural plant essential oils, and the activity is reduced when any one of the 4 natural plant essential oils is absent. The nanocapsule prepared from composition 5 has significantly higher hydroxyl radical scavenging rate than composition 5, because essential oil in compositions 1-5 is volatile and has short action time, and the prepared nanocapsule can overcome the defect and improve the hydroxyl radical scavenging activity.
3. And (4) conclusion: the compound essential oil and the nanocapsules thereof have high hydroxyl radical scavenging activity, which shows that the compound essential oil has strong oxidation resistance, and can smooth skin and prevent wrinkles after long-term use.
Test example 3
The oxygen free radical scavenging activity test of the compound essential oil of the invention comprises:
1. materials and methods
1.1 test materials
Suaeda salsa seed oil, pepper seed oil, citrus seed oil and Chinaberry fruit oil are respectively prepared according to the embodiment. Preparing compound essential oil (composition 1, lacking Chinaberry fruit oil) of Suaeda glauca seed oil, pricklyash peel seed oil and citrus seed oil according to the formula proportion; compound essential oil of suaeda salsa seed oil, pepper seed oil and chinaberry seed oil (composition 2, lacking of citrus seed oil); compound essential oil of suaeda glauca seed oil, citrus seed oil and chinaberry seed oil (composition 3, lacking of pepper seed oil); compound essential oil of pricklyash peel seed oil, orange seed oil and chinaberry seed oil (composition 4, lacking suaeda salsa seed oil); the essential oil missing in the composition is replaced by peanut oil with equal proportion. The invention is compounded with essential oil (composition 5); the compound essential oil nanocapsule (composition 6, nanocapsule prepared from composition 5); positive control vitamin c (vc) in water.
1.2 test methods
Adopts pyrogallol autoxidation method.
(1) Preparing and measuring pyrogallol solution: adding 2.95mL of Tris-HCl buffer solution into a quartz cuvette, adding about 0.50mL of pyrogallol solution, quickly and uniformly mixing, starting timing, measuring the absorbance value at 325nm, reading the value A once every 30s, stopping reading when 300 seconds, and carrying out parallel measurement for 3 times in each group;
(2) preparation and determination of sample solution: adding 0.50mL of sample solution into a quartz cuvette, sequentially adding 2.90mL of Tris-HCl buffer solution and 0.50mL of pyrogallol solution, quickly and uniformly mixing, starting timing, measuring the absorbance value at 325nm, reading the value A once every 30s, stopping reading when 300 seconds, and carrying out parallel measurement for 3 times in each group;
(3) preparing and measuring a VC solution: adding 0.50mLVC solution into a quartz cuvette, sequentially adding 2.90mLTris-HCl buffer solution and 0.50mL pyrogallol solution, quickly and uniformly mixing, starting timing, measuring the absorbance value at 325nm, reading the A value once every 30s, stopping reading when 300 seconds, and carrying out parallel measurement on each group for 3 times;
calculating the formula: clearance rate ([ Delta ] A)0-△AS)/△A0 x 100％；
In the formula, Delta A0Is the difference in absorbance at 325nm between 300s and 30s for the pyrogallol solution; delta ASIs the difference in absorbance at 325nm between 300s and 30s for the sample solution or VC solution.
2. Results and analysis
The oxygen radical clearance of each composition is shown in table 8.
TABLE 8 oxygen radical scavenging test results for each composition
In comparison with the composition 1, the composition,△P<0.01; in comparison with the composition 2, the present inventors have shown that,▲P<0.01，▲▲P<0.01; in comparison with the composition 3, the composition,＊P<0.01; in comparison with the composition 4, the composition,θP<0.01; in comparison with the composition No. 5,×P<0.01; in comparison with VC, the comparison is made,#P<0.01。
from table 7, each composition had oxygen radical scavenging activity, and composition 6 had the highest oxygen radical scavenging rate, comparable to VC. Compared with the compositions 1, 2, 3 and 4 respectively, the oxygen radical clearance rate of the composition 5 is obviously increased, which shows that the activity of the combination of the 4 natural plant essential oils is increased, and the activity of the combination is reduced if any one of the compositions is absent. The composition 5 can be used to prepare nanocapsules, which have higher oxygen free radical scavenging rate than composition 5, because essential oils in compositions 1-5 are volatile and have short action time, and the prepared nanocapsules can overcome the defect and improve oxygen free radical scavenging activity.
3. And (4) conclusion: the compound essential oil and the nanocapsule thereof have high oxygen radical scavenging activity, which shows that the compound essential oil has strong oxidation resistance, and can smooth skin and prevent wrinkles after long-term use.
Test example 4
The reducing power of the compound essential oil is measured and tested as follows:
1. materials and methods
1.1 test materials
Suaeda salsa seed oil, pepper seed oil, citrus seed oil and Chinaberry fruit oil are respectively prepared according to the embodiment. Preparing compound essential oil (composition 1, lacking Chinaberry fruit oil) of Suaeda glauca seed oil, pricklyash peel seed oil and citrus seed oil according to the formula proportion; compound essential oil of suaeda salsa seed oil, pepper seed oil and chinaberry seed oil (composition 2, lacking of citrus seed oil); compound essential oil of suaeda glauca seed oil, citrus seed oil and chinaberry seed oil (composition 3, lacking of pepper seed oil); compound essential oil of pricklyash peel seed oil, orange seed oil and chinaberry seed oil (composition 4, lacking suaeda salsa seed oil); the invention is compounded with essential oil (composition 5); the compound essential oil nanocapsule of the invention (composition 6, nanocapsule prepared from composition 5).
1.2 test methods
The reducing power is measured by adopting a potassium ferricyanide reduction method, a sample, 1 percent of potassium ferricyanide and 1mL of 0.2mol/L phosphate buffer solution are sequentially added into a test tube, the mixture is bathed in water at 50 ℃ for 25min, then the mixture is rapidly cooled, the temperature is reduced to 15 ℃, 10 percent of trichloroacetic acid solution is added, the mixture is evenly mixed and centrifuged, 1mL of supernatant is taken into the test tube, and 1mL of water and 0.4mL of 1 percent of FeCl are added3Standing for 10min at room temperature after uniform mixing, and finally measuring the absorbance value at the wavelength of 700nm by using an enzyme-labeling instrument, wherein each group is measured in parallel for 3 times, and the higher the absorbance is, the stronger the reduction capability is.
2. Results and analysis
The results of the reduction power measurement of each composition are shown in Table 9.
TABLE 9 test results of reduction power measurement of each composition
In comparison with the composition 1, the composition,△P<0.01; in comparison with the composition 2, the present inventors have shown that,▲P<0.01; and combinations ofThe comparison of the substance 3 is carried out,＊P<0.01; in comparison with the composition 4, the composition,θP<0.01; in comparison with the composition No. 5,×P<0.01。
as can be seen from Table 8, each of the compositions had the highest reducing activity, and composition 6 had the highest reducing activity. The increased reducing activity of composition 5 compared to compositions 1, 2, 3, and 4, respectively, indicates that the activity of the combination of 4 natural plant essential oils is increased and decreased in the absence of any one. The nanocapsule prepared from composition 5 has significantly higher reduction activity than composition 5, because the essential oils in compositions 1-5 are volatile and have short action time, and the nanocapsule prepared from composition 5 can overcome the defect and improve the reduction activity.
3. And (4) conclusion: the compound essential oil and the nanocapsules thereof have high reducing power, which shows that the compound essential oil has strong oxidation resistance, and can smooth skin and prevent wrinkles after long-term use.
Test example 5
Human body efficacy evaluation test of whitening, anti-wrinkle and moisturizing water, milk and cream
1. Test subjects: 20 (5 men, 15 women) volunteers 30-45 years old who were healthy and had no history of cosmetic allergy.
2. The test method comprises the following steps: the skin elasticity, melanin content and moisture content were measured using an MPA580 instrument, the average of the skin elasticity and melanin content of 20 volunteers who did not use the cosmetic of the present invention was used as a blank control, the measurement was performed once a week for 3 weeks, and the results were finally analyzed. 3. Results and analysis
The skin elasticity results are shown in Table 10.
TABLE 10 measurement results of skin elasticity
The melanin content results are shown in table 11.
TABLE 11 melanin content
The moisture content results are shown in Table 12.
TABLE 12 moisture content
From tables 10-12, after the skin whitening, anti-wrinkle and moisturizing lotion, cream and cream are used for 1 week, the elasticity and moisture content of the skin are obviously increased, the melanin content of the skin is obviously reduced, the effect is gradually increased in the using process, after the skin whitening, anti-wrinkle and moisturizing lotion, cream and cream are used for 3 weeks, the elasticity of the skin is increased by 24.66%, the melanin content is reduced by 18.83%, the moisture content is increased by 32.85%, and no local skin allergy, inflammatory reaction and the like are seen, so that the series of cosmetics are safe and effective.
4. And (4) conclusion: the detection result shows that the whitening anti-wrinkle moisturizing lotion, cream and cream have the effects of enhancing skin elasticity, inhibiting melanin and increasing skin water content, can whiten skin, resist wrinkle and delay aging after long-term use, and has no toxic or side effect.
Claims (5)
1. The compound essential oil is characterized by being prepared by combining suaeda salsa seed oil, zanthoxylum bungeanum seed oil, citrus seed oil and szechwan chinaberry fruit oil, wherein the ratio of the suaeda salsa seed oil to the zanthoxylum bungeanum seed oil to the citrus seed oil to the szechwan chinaberry fruit oil is 3-4: 1-2: 2-3: 5-6;
the preparation method of the suaeda salsa seed oil comprises the following steps:
taking suaeda salsa seeds, crushing, sieving, placing in a charging basket of microwave dynamic extraction equipment, adding 60 times of water, soaking for 45min at room temperature, setting the microwave power to 287W, performing microwave radiation for 30min, connecting a volatile oil separator on the microwave extraction equipment, and collecting distilled essential oil to obtain the suaeda salsa seed oil.
The preparation method of the pricklyash seed oil comprises the following steps:
pulverizing semen Zanthoxyli, sieving, and placing in supercritical CO2Adding 70% ethanol in an amount which is 2.5 times that of the extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 40MPaThe extraction temperature is 40 ℃, the extraction time is 3h, CO2The flow rate was 20 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain oleum Zanthoxyli Bungeani.
The preparation method of the citrus seed oil comprises the following steps:
pulverizing citrus seeds, sieving, and placing in supercritical CO2Adding 3 times of 70% ethanol into an extraction kettle, soaking for 150min at room temperature, wherein the extraction conditions are as follows: the extraction pressure is 35MPa, the extraction temperature is 45 ℃, the extraction time is 3h, and CO is2The flow rate was 25 kg/h. Filtering the extractive solution, recovering 70% ethanol with rotary evaporator, and volatilizing to obtain citrus seed oil.
The preparation method of the szechwan chinaberry fruit oil comprises the following steps:
pulverizing fructus Toosendan, sieving, placing in round bottom flask, adding 10 times of distilled water, adjusting pH to 4.5 with hydrochloric acid, adding 1% cellulase into container, slightly stirring, placing in water bath, performing enzymolysis at 50 deg.C for 35min, performing steam distillation, installing volatile oil extractor, heating to boil, distilling for 2.5 hr, and collecting distilled essential oil to obtain fructus Toosendan oil.
The suaeda salsa seed oil, the pepper seed oil, the citrus seed oil and the szechwan chinaberry fruit oil are selected according to the proportion and are uniformly mixed to obtain the compound essential oil.
2. The compound essential oil of claim 1, wherein the ratio of the suaeda salsa seed oil, the pepper seed oil, the citrus seed oil and the chinaberry seed oil is 3:1:2: 5.
3. A nanocapsule containing compound essential oil, which is characterized in that the nanocapsule containing 33% of the compound essential oil is prepared from the compound essential oil according to claim 1 by a reverse emulsification method.
4. The compound essential oil nanocapsule of claim 3, wherein the preparation method of the compound essential oil nanocapsule comprises:
taking 0.1g of acetyl chitosan, placing the acetyl chitosan in a beaker, and adding deionized water to 10mL to obtain an acetyl chitosan solution for later use; taking 10g of sodium alginate, placing the sodium alginate in a beaker, adding 10mL of compound essential oil, carrying out ultrasonic emulsification completely, slowly dropwise adding 0.5mol/LNaOH to adjust the pH value to 7.5 to obtain sodium alginate nanocapsules, washing and centrifuging the sodium alginate nanocapsules, adding the sodium alginate nanocapsules into an acetyl chitosan solution, and carrying out magnetic stirring for 3 hours to obtain the compound essential oil nanocapsules.
5. The application of the compound essential oil nanocapsule is characterized in that the compound essential oil nanocapsule of claim 3 is added into a whitening anti-wrinkle cosmetic according to the mass of the compound essential oil nanocapsule accounting for 10-15% of the mass of the whitening anti-wrinkle cosmetic;
the whitening anti-wrinkle cosmetic prepared from the compound essential oil nanocapsule has no special limitation on the selection of dosage form and matrix, and can be prepared into whitening anti-wrinkle moisturizing lotion, whitening anti-wrinkle milk, whitening anti-wrinkle cream and the like.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011182423.1A CN112266825A (en) | 2020-10-29 | 2020-10-29 | Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011182423.1A CN112266825A (en) | 2020-10-29 | 2020-10-29 | Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112266825A true CN112266825A (en) | 2021-01-26 |
Family
ID=74345360
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011182423.1A Pending CN112266825A (en) | 2020-10-29 | 2020-10-29 | Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112266825A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113576960A (en) * | 2021-06-17 | 2021-11-02 | 刘沙 | Preparation process of anti-wrinkle moisturizing emulsion for dry skin |
| CN114774195A (en) * | 2022-02-24 | 2022-07-22 | 玉溪中烟种子有限责任公司 | Preparation method of tobacco seed oil with whitening active ingredient, whitening activity evaluation method and application |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250678A (en) * | 2011-05-23 | 2011-11-23 | 井冈山茶油有限公司 | Method for extracting camellia oil by using microwave assisted aqueous extraction method |
| CN102666564A (en) * | 2009-11-19 | 2012-09-12 | 花王株式会社 | Dopa oxidase activity inhibitor and skin whitening agent |
| US20170157005A1 (en) * | 2016-10-26 | 2017-06-08 | IndagoMed. LLC | Novel Anti-Wrinkle and Anti-Aging Nano Formulations and Method of Preparation using Novel Nano Co-Delivery System |
| WO2018236007A1 (en) * | 2017-06-21 | 2018-12-27 | 주식회사 마린테크노 | Whitening cosmetic composition containing citrus junos seed extract as active ingredient |
| CN110384648A (en) * | 2019-07-17 | 2019-10-29 | 碱蓬高新科技(盘锦)有限公司 | A kind of facial mask and preparation method thereof |
| WO2020091291A1 (en) * | 2018-11-01 | 2020-05-07 | 대한민국(산림청 국립산림과학원장) | Composition for skin whitening comprising zanthoxylum coreanum nakai extract or fraction thereof as active ingredient |
-
2020
- 2020-10-29 CN CN202011182423.1A patent/CN112266825A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102666564A (en) * | 2009-11-19 | 2012-09-12 | 花王株式会社 | Dopa oxidase activity inhibitor and skin whitening agent |
| CN102250678A (en) * | 2011-05-23 | 2011-11-23 | 井冈山茶油有限公司 | Method for extracting camellia oil by using microwave assisted aqueous extraction method |
| US20170157005A1 (en) * | 2016-10-26 | 2017-06-08 | IndagoMed. LLC | Novel Anti-Wrinkle and Anti-Aging Nano Formulations and Method of Preparation using Novel Nano Co-Delivery System |
| WO2018236007A1 (en) * | 2017-06-21 | 2018-12-27 | 주식회사 마린테크노 | Whitening cosmetic composition containing citrus junos seed extract as active ingredient |
| WO2020091291A1 (en) * | 2018-11-01 | 2020-05-07 | 대한민국(산림청 국립산림과학원장) | Composition for skin whitening comprising zanthoxylum coreanum nakai extract or fraction thereof as active ingredient |
| CN110384648A (en) * | 2019-07-17 | 2019-10-29 | 碱蓬高新科技(盘锦)有限公司 | A kind of facial mask and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113576960A (en) * | 2021-06-17 | 2021-11-02 | 刘沙 | Preparation process of anti-wrinkle moisturizing emulsion for dry skin |
| CN114774195A (en) * | 2022-02-24 | 2022-07-22 | 玉溪中烟种子有限责任公司 | Preparation method of tobacco seed oil with whitening active ingredient, whitening activity evaluation method and application |
| CN114774195B (en) * | 2022-02-24 | 2023-09-26 | 玉溪中烟种子有限责任公司 | Preparation method and application of tobacco seed oil with whitening active ingredient |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103919711B (en) | A kind of anti-ageing whitening cream of oolong tea and manufacture method thereof | |
| JPH0717847A (en) | Skin external preparation | |
| KR101266180B1 (en) | Vegetable antibacterial composition and cosmetic composition for improving skin using the same | |
| JP5137457B2 (en) | Stem cell growth factor expression increase inhibitor | |
| KR101387308B1 (en) | Skin whitening composition by using of dendropanax morbifera ferment extract | |
| CN104814908A (en) | Mask with effects of resisting wrinkle and tendering skin | |
| KR20090002677A (en) | Cosmetic composition having antioxidation activity | |
| CN112006959B (en) | Antioxidant whitening cream containing dandelion and tea extracts and preparation method thereof | |
| CN105997827A (en) | Compound camellia oil moisturizer and preparation method thereof | |
| CN112266825A (en) | Compound essential oil, preparation method thereof and application of compound essential oil in whitening and anti-wrinkle cosmetics | |
| KR102049208B1 (en) | Cosmetic composition for reducing acne and inhibiting sebum secretion | |
| KR20080026800A (en) | Cosmetic composition | |
| KR101142541B1 (en) | Anti-wrinkle cosmetic composition containing oriental herb extract treated by enzyme and its extraction method | |
| KR100899278B1 (en) | Cosmetic composition | |
| KR101474340B1 (en) | Anti-aging cosmetic composition comprising herb ferment extract | |
| CN106137875B (en) | A kind of composition and facial mask | |
| KR20100121352A (en) | A composition comprising complex crude drug extract for anti-aging and improvement of skin | |
| CN110974775A (en) | Allergy-relieving composition containing nostoc sphaeroides and preparation method and application thereof | |
| KR20010077652A (en) | Compositions for whitening cosmetics | |
| KR20020019716A (en) | Cosmetic compositions containing garlic and Acanthopanax extracts | |
| KR101666783B1 (en) | Cosmetic composition comprising fermentates of herbal medicine fruits improving whitening and antioxidation | |
| KR100466379B1 (en) | Composition for skin whitening | |
| KR100534074B1 (en) | Cosmetic composition containing grain extract and fermented grain phellinus linteus extract, and cosmetics containing the same | |
| CN106420483A (en) | Skin-clearing blackhead dissolving solution | |
| JP2021095370A (en) | Panax ginseng seeds treated by method comprising steam heat treatment step and/or skin external or internal formulation containing extract thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210126 |
|
| RJ01 | Rejection of invention patent application after publication |