CN112249488A

CN112249488A - Kit for diagnosing cerebrospinal fluid difficult pathogen and diagnostic device thereof

Info

Publication number: CN112249488A
Application number: CN202011073799.9A
Authority: CN
Inventors: 朱峻; 杰西卡·朱
Original assignee: Shenzhen Senying Bio Tech Co ltd
Current assignee: Shenzhen Senying Bio Tech Co ltd
Priority date: 2020-10-09
Filing date: 2020-10-09
Publication date: 2021-01-22

Abstract

The invention discloses a kit for diagnosing cerebrospinal fluid difficult pathogen and a diagnostic device thereof, and relates to the technical field of medical treatment. According to the invention, the adjusting wheel can be rotated to enable the two clamping blocks to clamp or loosen the test tube, so that the test tube can be prevented from shaking, the lifting hand wheel can also be rotated to adjust the height of the supporting block, the placing requirements of test tubes with different lengths can be met, during diagnosis, pathogens can be determined by combining various comparison information, the accuracy of diagnosis can be improved, and the misdiagnosis rate can be reduced.

Description

Kit for diagnosing cerebrospinal fluid difficult pathogen and diagnostic device thereof

Technical Field

The invention relates to the technical field of medical treatment, in particular to a kit for diagnosing cerebrospinal fluid difficult pathogen and a diagnostic device thereof.

Background

Cerebrospinal fluid: cerebrospinal fluid is a colorless, transparent fluid that exists in the ventricles of the brain and in the subarachnoid space. The specific gravity is 1.005, and the total amount is 130-150 ml. The average daily production was 524 ml. The cerebrospinal fluid surrounds and supports the entire brain and spinal cord, effectively reducing the weight of the brain to 1/6, and protecting the trauma to some extent. Plays a role in eliminating metabolites and inflammatory exudates as well as in lymph fluid at other parts of the body.

At present, the central nervous infection, encephalitis and meningitis are mostly caused by various pathogens, so that the rapid detection of various pathogens is particularly important. But traditional kit is when using, directly arranges the test tube in the reagent box, and the test tube very easily rocks in the reagent box, very easily takes place to destroy, to the test tube of different length, places the back in the reagent box, highly differs, and is inconvenient to place, and simultaneously, traditional diagnostic device only can adopt single detection mode, judges the pathogen, very easily the deviation appears, and diagnostic accuracy is not good, is unfavorable for patient's treatment.

Therefore, a kit for diagnosing cerebrospinal fluid difficult pathogen and a diagnostic device thereof are needed, wherein the kit can fix a test tube, is convenient to take and place, and can improve the diagnostic accuracy.

Disclosure of Invention

The invention aims to solve the problems that a test tube is easy to shake, inconvenient to take and place and poor in diagnosis accuracy in the prior art, and provides a kit for diagnosing cerebrospinal fluid difficult pathogens and a diagnosis device thereof.

In order to achieve the purpose, the invention adopts the following technical scheme:

a kit for diagnosing cerebrospinal fluid difficult pathogen comprises a box body, wherein a limiting plate and a fixing plate are fixedly mounted in the box body, the limiting plate is positioned above the fixing plate, a test tube groove and a movable groove are formed in the fixing plate, a partition plate extends in the movable groove, a first connecting rod and a second connecting rod are respectively arranged in the movable groove in a sliding mode, the first connecting rod and the second connecting rod are respectively positioned on two sides of the test tube groove, limiting rods are embedded in the first connecting rod and the second connecting rod, two ends of each limiting rod are respectively fixed with the inner walls of the partition plate and the movable groove, pull rods are respectively fixedly mounted on corresponding sides of the first connecting rod and the second connecting rod, clamping blocks are fixedly mounted at one ends of the pull rods and extend into the test tube groove, a transverse lead screw is rotatably arranged in the movable groove and is in threaded connection with the first connecting rod, the smooth end of the transverse screw rod extends to the outer side of the box body and is fixedly provided with an adjusting wheel, a shaft lever is fixedly arranged in the movable groove, a gear is arranged on the shaft lever in a rotating mode, the corresponding sides of the first connecting rod and the second connecting rod are respectively and fixedly provided with a driving toothed bar and a driven toothed bar, the driving toothed bar and the driven toothed bar are respectively positioned on two sides of the gear, and the driving toothed bar is meshed with the driven toothed bar;

the top of limiting plate is located the below in test-tube groove and has seted up the lift groove, and goes up and down the inslot and inlay and be equipped with the supporting shoe, the supporting shoe internal rotation is provided with rotatory piece, the bottom of box is seted up flutedly, the bottom threaded connection of box has vertical lead screw, the smooth end of vertical lead screw extends to in the supporting shoe with rotatory piece fixed connection, the one end fixed mounting that vertical lead screw is located the recess has lifting hand wheel.

Furthermore, a slot is formed in the bottom of the inner wall of the box body, an inserting rod is embedded in the slot, and the inserting rod is fixedly connected with the supporting block.

Furthermore, an arc-shaped groove is formed in the top of the supporting block, and a protective pad is bonded on the inner wall of the arc-shaped groove.

Further, the top of box rotates through the hinge and is provided with the case lid, the same one side of case lid and box respectively fixed mounting have fixture block and buckle, and the fixture block is connected with the buckle block.

A cerebrospinal fluid suspected pathogen diagnostic device comprising:

and the information acquisition module is used for acquiring index information which is stored by using the test tubes in the kit and extracted from the cerebrospinal fluid according to the received instruction.

And the primer information storage module is used for storing information of various cerebrospinal fluid pathogen symptoms caused by each primer.

And the judgment module is used for comparing the acquired index information with the stored symptom information, and is electrically connected with the information acquisition module and the primer information storage module.

Further, the index information includes quantitative protein information, quantitative glucose information, quantitative chloride information, and enzymatic measurement information.

Further, the primer is various pathogenic bacteria causing abnormal symptoms of cerebrospinal fluid.

Further, the determination module includes:

and the first confirmation module is used for judging whether the acquired index information is positive or not.

And the second confirmation module is used for matching the acquired indication information with the primer information.

And the third confirming module is used for confirming that the obtained indication information does not have the primer.

Furthermore, the first confirmation module is electrically connected with the information acquisition module, and the first confirmation module is electrically connected with the second confirmation module and the third confirmation module.

Further, the second confirmation module is electrically connected with the primer information storage module.

Compared with the prior art, the invention provides a kit for diagnosing cerebrospinal fluid difficult pathogen and a diagnostic device thereof, which have the following beneficial effects:

1. when the test tube fixing device is used, a user can rotate the adjusting wheel by hand, the adjusting wheel drives the transverse screw rod to rotate, the first connecting rod on the transverse screw rod can move, the first connecting rod drives the right clamping block to move rightwards through the pull rod, meanwhile, the first connecting rod can drive the driving toothed bar to move rightwards, the gear is rotated, the driven toothed bar further moves leftwards, the second connecting rod is driven to move leftwards, the second connecting rod drives the left clamping block to move leftwards through the pull rod, the test tube can be taken out, the adjusting wheel can be rotated reversely when the test tube is fixed, the transverse screw rod can rotate reversely, the first connecting rod and the second connecting rod are close to each other, the two clamping blocks are driven to clamp the test tube, the test tube can be effectively prevented from shaking, and the test tube is protected.

2. When the test tube lifting device is used, a user can screw the lifting hand wheel according to requirements, the lifting hand wheel drives the vertical screw rod to rotate in the box body, so that the supporting block moves upwards, the inserting rod can be driven to slide in the slot when the supporting block moves, the supporting block can be prevented from rotating, the position of the bottom of the test tube can be adjusted along with the upward or downward movement of the supporting block, and the use requirements of test tubes with different lengths are met.

3. When the pathogen is diagnosed, index information extracted from cerebrospinal fluid and stored by using a test tube in a kit is acquired, the acquired information is transmitted to a judging module, the first determining module judges whether the index information is negative or positive, if the index information is negative, the judging information is transmitted to a third determining module, the acquired indicating information is indicated to be free of a primer, if the index information is positive, the judging information is transmitted to a second determining module, the second determining module acquires information from a primer information storage module, information matching of protein, glucose determination, chloride and various enzymes is carried out, and the pathogen is determined.

Drawings

FIG. 1 is a schematic diagram showing the construction of a kit for diagnosing cerebrospinal fluid stubborn pathogens according to the present invention;

FIG. 2 is a sectional view of a fixing plate of a kit for cerebrospinal fluid difficult pathogen diagnosis according to the present invention;

FIG. 3 is a top view of a fixing plate of a kit for cerebrospinal fluid difficult pathogen diagnosis according to the present invention;

FIG. 4 is an enlarged view of the kit for cerebrospinal fluid difficult pathogen diagnosis of the present invention at A in FIG. 2;

fig. 5 is a schematic diagram showing the components of a cerebrospinal fluid suspected pathogen diagnostic device according to the present invention.

The reference numbers in the figures illustrate: 1. a box body; 101. a groove; 2. a box cover; 3. a clamping block; 4. buckling; 5. a slot; 6. a limiting plate; 7. a fixing plate; 701. a test tube groove; 702. a movable groove; 8. a support block; 9. inserting a rod; 10. a vertical screw rod; 11. lifting a hand wheel; 12. a partition plate; 13. a clamping block; 14. a pull rod; 15. a second connecting rod; 16. a limiting rod; 17. a first connecting rod; 18. a driving rack bar; 19. a transverse screw rod; 20. an adjustment wheel; 21. a driven rack bar; 22. a gear; 23. an information acquisition module; 24. a primer information storage module; 25. and a judging module.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; the two components can be directly connected or indirectly connected through an intermediate medium, and the two components can be communicated with each other; the specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1-4, a kit for diagnosing cerebrospinal fluid difficult pathogen includes a case 1, a limiting plate 6 and a fixing plate 7 are fixedly installed in the case 1, the limiting plate 6 is located above the fixing plate 7, a test tube slot 701 and a movable slot 702 are formed in the fixing plate 7, a partition plate 12 extends in the movable slot 702, a first connecting rod 17 and a second connecting rod 15 are respectively slidably installed in the movable slot 702, the first connecting rod 17 and the second connecting rod 15 are respectively located at two sides of the test tube slot 701, a limiting rod 16 is embedded in the first connecting rod 17 and the second connecting rod 15, two ends of the limiting rod 16 are respectively fixed with inner walls of the partition plate 12 and the movable slot 702, pull rods 14 are respectively and fixedly installed at corresponding sides of the first connecting rod 17 and the second connecting rod 15, a clamping block 13 is fixedly installed in the test tube slot 701 as one end of the pull rod 14 extends, a transverse screw 19 is rotatably installed in the movable slot 702, the transverse screw rod 19 is in threaded connection with the first connecting rod 17, the smooth end of the transverse screw rod 19 extends to the outer side of the box body 1 and is fixedly provided with an adjusting wheel 20, a shaft rod is fixedly arranged in the movable groove 702 and is provided with a gear 22 in a rotating manner, the corresponding sides of the first connecting rod 17 and the second connecting rod 15 are respectively and fixedly provided with a driving toothed bar 18 and a driven toothed bar 21, the driving toothed bar 18 and the driven toothed bar 21 are respectively positioned at two sides of the gear 22, and the driving toothed bar 18 and the driven toothed bar 21 are meshed and connected, when in use, a test tube can be placed between the two clamping blocks 13, the adjusting wheel 20 is rotated by hand, the adjusting wheel 20 can drive the transverse screw rod 19 to rotate, the first connecting rod 17 on the transverse screw rod 19 can move, the first connecting rod 17 can drive the clamping block 13 at the right side to move leftwards through the pull rod 14, when the first connecting rod 17 moves, the driving, drive gear 22 and rotate, along with gear 22's rotation, can make driven rack bar 21 remove right, driven rack bar 21 drives second connecting rod 15 and removes right for left clamp splice 13 removes right, realizes the centre gripping to the test tube, can effectually avoid the test tube to rock in fixed plate 7, when the demand is relieved the fixed to the test tube, can the antiport regulating wheel 20, alright keep away from mutually in order to make two clamp splices 13, alright in order to relieve the fixed to the test tube.

Referring to fig. 1-2, a lifting groove is formed in the top of the limiting plate 6 below the test tube groove 701, a supporting block 8 is slidably embedded in the lifting groove, a rotating block is rotatably arranged in the supporting block 8, a groove 101 is formed in the bottom of the box body 1, a vertical lead screw 10 is connected to the bottom of the box body 1 through a thread, a smooth end of the vertical lead screw 10 extends into the supporting block 8 and is fixedly connected with the rotating block, a lifting hand wheel 11 is fixedly mounted at one end of the vertical lead screw 10 in the groove 101, a slot 5 is formed in the bottom of the inner wall of the box body 1, an inserting rod 9 is embedded in the slot 5 and is fixedly connected with the supporting block 8, an arc-shaped groove is formed in the top of the supporting block 8, a protective pad is bonded on the inner wall of the arc-shaped groove, a box cover 2 is rotatably arranged at the top of the box, the clamping block 3 is connected with the buckle 4 in a clamping manner, a user can screw the lifting hand wheel 11 according to requirements, the lifting hand wheel 11 drives the vertical screw rod 10 to rotate in the box body 1, the supporting block 8 is enabled to move upwards, when the supporting block 8 moves, the inserting rod 9 can be driven to slide in the slot 5, the supporting block 8 can be prevented from rotating, the position of the bottom of the test tube can be adjusted along with the upward or downward movement of the supporting block 8, the use requirements of test tubes with different lengths can be met, the box cover 2 can be conveniently fixed and opened through the arranged clamping block 3 and the buckle 4, when the kit is used, the storage condition of the kit is light-proof storage, and pollution is avoided during transportation at; long-term storage at-20 deg.C.

Referring to fig. 1-5, a cerebrospinal fluid suspected pathogen diagnostic device comprises:

the information acquisition module 23 is used for acquiring index information extracted from cerebrospinal fluid and stored by using a test tube in the kit according to the received instruction, wherein the index information comprises protein quantitative information, glucose determination information, chloride determination information and enzymology determination information, the adult protein waist pool is 400mg/L, the cerebellum medulla oblongata pool is 100 mg/L, 250mg/L and the ventricle is 50-150mg/L, the protein content of the cerebrospinal fluid is increased, the damage of the blood brain barrier is prompted, the diseases are commonly seen in brain, spinal cord and meningitis, tumors, hemorrhage and the like, and brain block, brain degeneration diseases, diseases causing cerebrospinal fluid circulation obstruction diseases and the like, when the protein in the cerebrospinal fluid is more than 10g/L, the cerebrospinal fluid is solidified in a yellow jelly shape after flowing out, and also has a protein-cell separation phenomenon which is clinically called as a Froin syndrome and is a characteristic of the subarachnoid cerebrospinal fluid, normal glucose assay values: 2.5-4.4mmol/L, blood sugar content, blood brain barrier integrity and glycolysis degree of sugar in cerebrospinal fluid are main factors influencing the sugar content in cerebrospinal fluid, the chloride measurement is normal 120 + 130mmol/L, the concentration of cerebrospinal fluid chloride ion is about 1.2-1.3 times of blood chloride, and bacterial or fungal meningitis is common in purulent meningitis, tuberculous meningitis and fungal meningitis. Especially in tubercular meningitis, the chloride reduction in CSF is particularly significant and occurs earlier than the glucose reduction, so chloride determination is an important diagnostic modality for cerebrospinal fluid difficult pathogen diagnosis, and enzymatic determination is also an important diagnostic modality for cerebrospinal fluid difficult pathogen diagnosis, such as lysozyme: in bacterial meningitis, the lysozyme content in CSF is increased most obviously, and the content of lysozyme in CSF is measured for brain identification and prognosis judgment, wherein adenosine dehydrogenase ADA: the ADA in the cerebrospinal fluid of the tuberculous meningitis patient is increased to the greatest extent, and the diagnostic kit can be used for diagnosis and differential diagnosis of tuberculous meningitis, is judged by combining various information, can improve the accuracy of diagnosis, and reduces the misdiagnosis rate.

The primer information storage module 24 is used for storing information of various cerebrospinal fluid pathogen symptoms caused by the primers, the primers are various pathogenic bacteria causing cerebrospinal fluid abnormal symptoms, and the pathogenic bacteria are escherichia coli, enterococcus faecalis, streptococcus pneumoniae, serratia marcescens, proteus mirabilis, stenotrophomonas maltophilia, acinetobacter baumannii, pseudomonas aeruginosa, enterococcus faecium, klebsiella pneumoniae, staphylococcus aureus, citrobacter buchneri, burkholderia cepacia, staphylococcus epidermidis and the like.

A determination module 25, configured to compare the acquired indicator information with the stored symptom information, where the determination module 25 is electrically connected to the information acquisition module 23 and the primer information storage module 24, and the determination module 25 includes: a first confirming module for judging whether the obtained index information is positive or not, a second confirming module for matching the obtained indication information with primer information, a third confirming module for confirming that no primer exists in the obtained indication information, the first confirming module is electrically connected with the information obtaining module 23, the first confirming module is electrically connected with the second confirming module and the third confirming module, the second confirming module is electrically connected with the primer information storage module 24, when the pathogen is diagnosed, the index information extracted from cerebrospinal fluid stored by using a test tube in the kit is obtained, the obtained information is transmitted to the judging module 24, the first confirming module judges whether the index information is positive or negative, when the index information is negative, the judging information is transmitted to the third confirming module, the obtained indication information is indicated that no primer exists, and when the index information is positive, the judging information is transmitted to the second confirming module, the second determination module obtains information from the primer information storage module 25, matches the information of protein, glucose, chloride and various enzymes, and determines pathogens, the control mode of the invention is controlled by manually starting and closing a switch, and the wiring diagram of the power element and the power supply belong to common knowledge in the field, so the control mode and the wiring arrangement are not explained in detail in the invention.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the equivalent replacement or change according to the technical solution and the modified concept of the present invention should be covered by the scope of the present invention.

Claims

1. The utility model provides a kit for diagnosis of cerebrospinal fluid difficult pathogen, includes box (1), its characterized in that, box (1) internal fixation installs limiting plate (6) and fixed plate (7), just limiting plate (6) are located the top of fixed plate (7), set up test tube groove (701) and activity groove (702) in fixed plate (7), it has baffle (12) to extend in activity groove (702), it is provided with head rod (17) and second connecting rod (15) to slide respectively in activity groove (702), and head rod (17) and second connecting rod (15) are located the both sides of test tube groove (701) respectively to all inlay in head rod (17) and second connecting rod (15) and be equipped with gag lever post (16), the both ends of gag lever post (16) are fixed with the inner wall of baffle (12) and activity groove (702) respectively, the equal fixed mounting in the corresponding side of head rod (17) and second connecting rod (15) has pull rod (14) ) One end of a pull rod (14) extends into a test tube groove (701) and is fixedly provided with a clamping block (13), a transverse screw rod (19) is arranged in the movable groove (702) in a rotating mode, the transverse screw rod (19) is in threaded connection with a first connecting rod (17), the smooth end of the transverse screw rod (19) extends to the outer side of the box body (1) and is fixedly provided with an adjusting wheel (20), a shaft rod is fixedly arranged in the movable groove (702), a gear (22) is arranged on the shaft rod in a rotating mode, the corresponding sides of the first connecting rod (17) and the second connecting rod (15) are respectively and fixedly provided with a driving toothed bar (18) and a driven toothed bar (21), the driving toothed bar (18) and the driven toothed bar (21) are respectively located on two sides of the gear (22), and the driving toothed bar (18) is in meshed connection with the driven toothed bar (21);

the utility model discloses a test tube box, including limiting plate (6), supporting shoe (8), the internal rotation of supporting shoe (8) is provided with rotatory piece, the bottom of box (1) is seted up flutedly (101), the bottom threaded connection of box (1) has vertical lead screw (10), the smooth end of vertical lead screw (10) extend to in supporting shoe (8) with rotatory piece fixed connection, the one end fixed mounting that vertical lead screw (10) are located recess (101) has lifting hand wheel (11).

2. The kit for diagnosing cerebrospinal fluid difficult pathogen according to claim 1, characterized in that the bottom of the inner wall of the box body (1) is provided with a slot (5), an inserted bar (9) is embedded in the slot (5), and the inserted bar (9) is fixedly connected with the supporting block (8).

3. The kit for diagnosing cerebrospinal fluid difficult pathogen according to claim 1, characterized in that the top of the supporting block (8) is provided with an arc-shaped groove, and the inner wall of the arc-shaped groove is adhered with a protective pad.

4. The kit for diagnosing the cerebrospinal fluid stubborn pathogen according to claim 1, wherein a case cover (2) is rotatably arranged at the top of the case body (1) through a hinge, a clamping block (3) and a clamping buckle (4) are respectively and fixedly arranged at the same side of the case cover (2) and the case body (1), and the clamping block (3) is connected with the clamping buckle (4) in a clamping manner.

5. The cerebrospinal fluid difficult pathogen diagnostic device according to claim 1, based on the kit for cerebrospinal fluid difficult pathogen diagnosis according to any one of claims 1 to 4, characterized by comprising:

the information acquisition module (23) is used for acquiring index information extracted from cerebrospinal fluid stored by using a test tube in the kit according to the received instruction;

a primer information storage module (24) for storing information that each primer causes various cerebrospinal fluid pathogen symptoms;

and the judging module (25) is used for comparing the acquired index information with the stored symptom information, and the judging module (25) is electrically connected with the information acquiring module (23) and the primer information storing module (24).

6. The apparatus of claim 5, wherein the index information comprises quantitative protein information, quantitative glucose information, quantitative chloride information, and enzymatic information.

7. The cerebrospinal fluid difficult pathogen diagnostic device according to claim 5, wherein the primers are various pathogenic bacteria causing abnormal symptoms of cerebrospinal fluid.

8. A cerebrospinal fluid suspected pathogen diagnostic device according to claim 5, characterized in that said decision module (25) comprises:

the first confirmation module is used for judging whether the acquired index information is positive or not;

the second confirmation module is used for matching the acquired indication information with the primer information;

9. The cerebrospinal fluid suspected pathogen diagnostic device according to claim 8, wherein the first confirmation module is electrically connected to the information acquisition module (23), and the first confirmation module is electrically connected to the second confirmation module and the third confirmation module.

10. The cerebrospinal fluid suspected pathogen diagnostic device according to claim 8, wherein the second confirmation module is electrically connected to the primer information storage module (24).