CN112239724A

CN112239724A - Biological cell experimental sample culture apparatus convenient for experimenters to observe

Info

Publication number: CN112239724A
Application number: CN202011118604.8A
Authority: CN
Inventors: 齐建明
Original assignee: Guangzhou Shuotai Technology Co ltd
Current assignee: Guangzhou Shuotai Technology Co ltd
Priority date: 2020-10-19
Filing date: 2020-10-19
Publication date: 2021-01-19

Abstract

The invention relates to the technical field of biological cells and discloses a biological cell experimental sample culture device convenient for an experimenter to observe, which comprises a shell, wherein the inner side of the shell is movably connected with a first rotating shaft, the outer part of the first rotating shaft is movably connected with a movable block, the outer part of the movable block is movably connected with a connecting block, the outer part of the connecting block is movably connected with a first rotating disc, the outer part of the first rotating disc is movably connected with a first shell, and the outer part of the first shell is fixedly connected with a first connecting rod; through the interact of all structures, can be so that the inside thermally equivalent of device, avoid the inside uneven temperature that leads to each position of device of being heated of device different, and then make the growth situation of cell have the difference, the experimenter need not to take out the cell culture dish under the unnecessary condition, just can observe the growth situation of cell, avoid cell culture dish and outside air contact too much to lead to the cell culture dish to be contaminated, and then lead to the fact the death of cell, influence the process of experiment.

Description

Biological cell experimental sample culture apparatus convenient for experimenters to observe

Technical Field

The invention relates to the technical field of biological cells, in particular to a biological cell experimental sample culture device convenient for experimenters to observe.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to make cells survive, grow, reproduce and maintain main structure and function. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and cell signal transduction, cell anabolism, cell growth and proliferation and the like can be researched.

Regardless of the whole bioengineering technology or one of them biological cloning technology, cell culture is an indispensable process, but the inside of the equipment is heated unevenly when the heater in most cell culture equipment is heating at present, so that the temperature of different positions in the device is different, and then the cell growth condition is different, the culture dish needs to be taken out of the device for observation by an inspector during observation, and the culture dish needs to be put back after the observation is finished, so that bacteria in the outside air easily enter the culture device when the culture dish is taken out and put back, the culture dish is polluted, and the culture dish is easily infected by the outside bacteria when the culture dish is observed, and the cell culture fails.

Therefore, in order to solve these problems, a device for culturing a sample for biological cell experiment, which is convenient for an experimenter to observe, is needed

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a biological cell experimental sample culture device convenient for experimenters to observe, which has the advantages of uniform heating and convenient observation, and solves the problems of cell culture failure caused by nonuniform heating and easy bacterial infection during observation.

(II) technical scheme

In order to realize the purposes of uniform heating and convenient observation, the invention provides the following technical scheme: a biological cell experimental sample culture device convenient for an experimenter to observe comprises a shell, wherein the inner side of the shell is movably connected with a first rotating shaft, the outer part of the first rotating shaft is movably connected with a movable block, the outer part of the movable block is movably connected with a connecting block, the outer part of the connecting block is movably connected with a first rotating disc, the outer part of the first rotating disc is movably connected with a first connecting rod, the outer part of the first connecting rod is fixedly connected with a heater, the inner side of the shell is fixedly connected with a transfusion tube, the outer part of the transfusion tube is movably connected with a rotating sleeve, the outer part of the rotating sleeve is fixedly connected with a second rotating disc, the inner part of the second rotating disc is fixedly connected with a second shell, lubricating oil is arranged in the second shell, the inner part of the second shell is movably connected with a magnetic plate, the outer, the outer portion of the rotating shaft II is fixedly connected with a connecting rod II, the outer portion of the connecting rod II is movably connected with a sliding block I, the outer portion of the sliding block I is movably connected with a connecting rod III, the inner side of the connecting rod III is movably connected with a sliding block II, the outer portion of the sliding block II is movably connected with a movable rod, the inner portion of the movable rod is movably connected with a screw rod, and the outer portion of the screw rod is movably connected with a.

Preferably, the movable blocks are two, the two movable blocks are the same in specification and corresponding in position, a first spring is arranged between the two movable blocks, the first spring is two, the two springs are corresponding in position, the first connecting rod is two, the two connecting rods are the same in specification and corresponding in position, a delivery pump is fixedly connected to the outside of the infusion tube, and a spray head is fixedly connected to the outside of the infusion tube.

Preferably, the number of the heaters is not less than four, the four heaters are the same in specification and correspond in position, a connecting rod four is fixedly connected to the outer portion of the first rotating shaft, the heater is also fixedly connected to the outer portion of the connecting rod four, a motor I is fixedly connected to the outer portion of the first rotating shaft, a motor II is fixedly connected to the outer portion of the second rotating shaft, the motor I and the motor II are fixedly connected to the outer portion of the shell, two-gear speed change can be achieved through the motor I, and the first gear is 75r/min and the second gear is 150 r.

Preferably, the bottom plate mainly comprises bottom plate one and bottom plate two, bottom plate one is no less than four and the outside at carousel two of angular distribution such as, bottom plate two is no less than ten and the outside at carousel two of angular distribution such as, the bottom plate is closer to the transfer line than bottom plate two, the inside also fixedly connected with magnetic sheet of bottom plate, the magnetic sheet of bottom plate inside and the magnetic sheet position of casing two inside are corresponding, the outside fixedly connected with spring two of bottom plate, the one end fixed connection that the bottom plate was kept away from to spring two is in the inside of carousel two, the outside swing joint of slider three has connecting rod five, the one end fixedly connected with electric chuck of slider three is kept away from to connecting rod five, the outside of slider three also is provided with.

Preferably, the inside fixedly connected with electro-magnet of carousel II, the outside fixedly connected with spring three of electro-magnet, the one end fixed connection of electro-magnet is kept away from to spring three in the inside of iron plate, and iron plate fixed connection is in the inside of fixture block, the specification of fixture block and the specification phase-match of culture dish.

Preferably, the outside of the magnetic plate is movably connected with lubricating oil, a spring IV is arranged inside the shell II, a contact I is arranged inside the shell II, the specifications of the contact I and the magnetic plate inside the shell II are matched, the specifications of the contact I and the movable rod are matched, a contact II is arranged outside the movable rod, the number of the contacts II is two, and the specifications of the two contacts II are the same and the positions of the two contacts II are corresponding.

Preferably, the screw and the rotating sleeve are electrically connected with a driving power supply, the electric sucker, the delivery pump, the rotating shaft II, the motor I and the electromagnet are electrically connected with an external power supply, and the contact I, the contact II, the external power supply and the driving power supply are electrically connected with the control center.

(III) advantageous effects

Compared with the prior art, the invention provides a biological cell experimental sample culture device convenient for experimenters to observe, which has the following beneficial effects:

1. this biological cell sample culture apparatus convenient to laboratory technician observes, through the interact of all structures, can be so that the inside thermally equivalent of device, avoid the inside uneven temperature that leads to each position of device of being heated of device different, and then make the growth situation of cell have the difference, laboratory technician need not to take out the cell culture dish under the unnecessary condition, just can observe the growth situation of cell, avoid cell culture dish and external air contact too much to lead to the cell culture dish to be polluted, and then cause the death of cell, influence the process of experiment.

2. This biological cell sample culture apparatus convenient to laboratory technician observes, through pivot two, connecting rod two, slider one, connecting rod three, slider two, movable rod, screw rod, slider three, connecting rod five and the electric chuck between the interact, can be when the cell growth condition is observed to needs, the cell culture dish of taking and remove to the position of being convenient for observe, reduce opening device's number of times, reduce the possibility that the cell culture dish is infected by external bacterium for cell culture can go on smoothly.

Drawings

FIG. 1 is a schematic partial cross-sectional view of the present invention;

FIG. 2 is an enlarged view taken at A in FIG. 1 according to the present invention;

FIG. 3 is an enlarged view of the invention at B of FIG. 2;

FIG. 4 is a schematic view of the connection relationship among a second rotating shaft, a second connecting rod, a first sliding block, a third connecting rod, a second sliding block, a movable rod, a screw and a third sliding block;

FIG. 5 is a schematic view of the connection relationship between the first rotating shaft, the first movable block, the first rotating disk and the first housing according to the present invention;

fig. 6 is a schematic view of the connection relationship between the movable block and the connection block according to the present invention.

In the figure: 1. a housing; 2. a first rotating shaft; 3. a movable block; 4. connecting blocks; 5. a first rotating disc; 6. a first shell; 7. a first connecting rod; 8. a heater; 9. a transfusion tube; 10. rotating the sleeve; 11. a second rotating disc; 12. a second shell; 13. lubricating oil; 14. a magnetic plate; 15. a base plate; 16. a clamping block; 17. a second rotating shaft; 18. a second connecting rod; 19. a first sliding block; 20. a third connecting rod; 21. a second sliding block; 22. a movable rod; 23. a screw; 24. and a third sliding block.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-6, a biological cell experimental sample culture device convenient for an experimenter to observe comprises a shell 1, wherein the inner side of the shell 1 is movably connected with a first rotating shaft 2, the outer part of the first rotating shaft 2 is movably connected with a movable block 3, the number of the movable blocks 3 is two, the specifications of the two movable blocks 3 are the same and the positions of the two movable blocks are corresponding, a first spring is arranged between the two movable blocks 3, the number of the first spring is two, the positions of the two springs are corresponding, the number of a first connecting rod 7 is two, the specifications of the two first connecting rods 7 are the same and the positions of the two connecting rods are corresponding, a delivery pump is fixedly connected; the external swing joint of movable block 3 has connecting block 4, the external swing joint of connecting block 4 has carousel one 5, the external swing joint of carousel one 5 has casing one 6, the external fixedly connected with connecting rod one 7 of casing one 6, the external fixedly connected with heater 8 of connecting rod one 7, heater 8 is no less than four, four 8 specifications of heater are the same and the position is corresponding, the external fixedly connected with connecting rod four of pivot one 2, the outside also fixedly connected with heater 8 of connecting rod four, the external fixedly connected with motor one of pivot one 2, the external fixedly connected with motor two of pivot two 17, motor one and motor two are all fixed connection in the outside of shell 1, two grades of variable speed can be realized to motor one, one grade is 75r/min and two grades are 150 r/min.

The inner side of the shell 1 is fixedly connected with a transfusion tube 9, the outer part of the transfusion tube 9 is movably connected with a rotating sleeve 10, the outer part of the rotating sleeve 10 is fixedly connected with a second rotating disc 11, the inner part of the second rotating disc 11 is fixedly connected with an electromagnet, the outer part of the electromagnet is fixedly connected with a third spring, one end of the third spring, far away from the electromagnet, is fixedly connected to the inner part of an iron block, the iron block is fixedly connected to the inner part of a clamping block 16, and the specification of the clamping block 16 is; a second shell 12 is fixedly connected to the inside of the second rotary table 11, lubricating oil 13 is movably connected to the inside of the second shell 12, a magnetic plate 14 is movably connected to the inside of the second shell 12, the outside of the magnetic plate 14 is movably connected with the lubricating oil 13, a fourth spring is arranged inside the second shell 12, a first contact is arranged inside the second shell 12, the first contact is matched with the magnetic plate 14 inside the second shell 12 in specification, the first contact is matched with the movable rod 22 in specification, a second contact is arranged outside the movable rod 22, the number of the second contacts is two, and the two second contacts are the same in specification and correspond in position; the outside swing joint of carousel two 11 has bottom plate 15, bottom plate 15 mainly comprises bottom plate one and bottom plate two, bottom plate one is no less than four and the equal angular distribution in the outside of carousel two 11, bottom plate two is no less than ten and the equal angular distribution in the outside of carousel two 11, bottom plate one is closer to transfer line 9 than bottom plate two, also fixedly connected with magnetic sheet 14 in the inside of bottom plate 15, magnetic sheet 14 in the inside of bottom plate 15 and the magnetic sheet 14 position of casing two 12 inside are corresponding, bottom plate 15's outside fixedly connected with spring two, the one end fixed connection that bottom plate 15 was kept away from to spring two is in the inside of carousel two 11, slider three 24's outside swing joint has connecting rod five, slider three 24's one end fixedly connected with electric suction cup is kept away from to connecting rod five, slider three 24's.

The outer part of the second rotating disc 11 is movably connected with a clamping block 16, the inner side of the shell 1 is movably connected with a second rotating shaft 17, the outer part of the second rotating shaft 17 is fixedly connected with a second connecting rod 18, the outer part of the second connecting rod 18 is movably connected with a first sliding block 19, the outer part of the first sliding block 19 is movably connected with a third connecting rod 20, the inner side of the third connecting rod 20 is movably connected with a second sliding block 21, the outer part of the second sliding block 21 is movably connected with a movable rod 22, the inner part of the movable rod 22 is movably connected with a screw 23, the outer part of the screw 23 is movably connected with a third sliding block 24, the screw 23 and the rotating sleeve 10 are electrically connected with a driving power supply, an electric suction cup, a conveying pump, the second rotating shaft 17, a second motor; through the interaction of all the structures, the interior of the device can be uniformly heated, the temperature difference of each part in the device caused by nonuniform heating in the device is avoided, the growth condition of the cells is further caused to be different, and the experimenter can observe the growth condition of the cells without taking out the cell culture dish under the unnecessary condition, so that the cell culture dish is prevented from being polluted due to excessive contact between the cell culture dish and the outside air, the cells are further killed, and the experiment progress is influenced; through the interaction between the second rotating shaft 17, the second connecting rod 18, the first sliding block 19, the third connecting rod 20, the second sliding block 21, the movable rod 22, the screw 23, the third sliding block 24, the fifth connecting rod and the electric sucker, when the cell growth condition needs to be observed, the cell culture dish can be taken and moved to a position convenient for observation, the times of opening the device are reduced, the possibility that the cell culture dish is infected by external bacteria is reduced, and the cell culture can be smoothly carried out.

The working principle is that in an initial state, the electromagnet is electrified and attracts an iron block in the fixture block 16, the spring III is compressed, an experimenter places a cell culture dish on the bottom plate 15, the bottom plate 15 moves downwards under the action of gravity of the cell culture dish, the spring II is compressed, the bottom plate 15 moves to drive the magnetic plate 14 in the bottom plate 15 to move, the magnetic plate 14 in the bottom plate 15 moves to enable the magnetic plate 14 in the shell II 12 to move under repulsive force, the spring IV is compressed and can trigger the contact I in the shell II 12 due to the movement of the magnetic plate 14 in the shell II 12, the control center can control an external power supply to stop supplying power to the electromagnet, the electromagnet is powered off, the fixture block 16 moves and fixes the cell culture dish under the action of the restoring force of the spring III, the experimenter controls the driving power supply to drive the rotation sleeve 10 to rotate through the control center.

When heating is needed, if only a cell culture dish is arranged outside the first bottom plate, an experimenter controls an external power supply to supply power to the first motor through a control center, so that the first motor runs at 75r/min and drives a first rotating shaft 2 to rotate, the first rotating shaft 2 rotates and drives a movable block 3 to rotate, the movable block 3 rotates and drives a connecting block 4 to move, the connecting block 4 moves and drives a first rotating disc 5 to rotate, the first rotating shaft 2 rotates and drives a fourth connecting rod to rotate, the fourth connecting rod rotates and drives a heater 8 to move, if cell culture dishes are arranged outside the first bottom plate and the second bottom plate, the experimenter controls the external power supply to supply power to the first motor through the control center, the first motor runs at 150r/min and drives the first rotating shaft 2 to rotate, the first rotating shaft 2 drives the movable block 3 to rotate while keeping away from the first rotating shaft 2 under the action of centrifugal force, and the movable block 3 rotates, connecting block 4 motion drives carousel 5 and rotates, and carousel 5 rotates and drives casing 6 and rotate, and casing 6 rotates and drives connecting rod 7 and rotate, and connecting rod 7 rotates and drives the motion of heater 8, and when needs add the culture solution, the experimenter passes through control maincenter control external power supply and gives the delivery pump power supply, and the delivery pump operation is carried the culture solution to the shower nozzle and is followed the shower nozzle blowout through transfer line 9.

When an experimenter needs to observe, the experimenter controls an external power supply to supply power to a second motor through a control center, the second motor operates to drive a second rotating shaft 17 to rotate, the driving power supply is controlled to stop driving a rotating sleeve 10, the second rotating shaft 17 rotates to drive a second connecting rod 18 to move, the second connecting rod 18 moves to drive a first sliding block 19 to move, the first sliding block 19 moves to drive a third connecting rod 20 to move, the third connecting rod 20 moves to drive a second sliding block 21 to move, the second sliding block 21 moves to drive a movable rod 22 to move, when a second contact outside the movable rod 22 is contacted with a cell culture dish, the control center controls the external power supply not to supply power to the second motor, the external power supply is controlled to supply power to an electromagnet, the electromagnet is electrified to attract an iron block inside a clamping block 16 to move, the control center controls the external power supply to supply power to an electric sucker, the electric sucker is electrified, the screw 23 rotates to drive the slider three 24 to move, the slider three 24 moves to drive the connecting rod five to move, the connecting rod five moves to drive the cell culture dish to move, when the contact I outside the slider three 24 contacts with the movable rod 22, the control center can control the driving power supply to stop driving the screw 23, meanwhile, the control center can control the external power supply to supply power to the motor two, the motor two operates to drive the rotating shaft two 17 to rotate, similarly, when the contact II of the corresponding position outside the movable rod 22 contacts with the shell 1, the control center can control the external power supply to no longer supply power to the motor two, and thus, the cell culture dish is brought to the observed position.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a biological cell experiment sample culture apparatus convenient to laboratory technician observes, includes shell (1), its characterized in that: the inner side of the shell (1) is movably connected with a first rotating shaft (2), the outer part of the first rotating shaft (2) is movably connected with a movable block (3), the outer part of the movable block (3) is movably connected with a connecting block (4), the outer part of the connecting block (4) is movably connected with a first rotating disc (5), the outer part of the first rotating disc (5) is movably connected with a first shell (6), the outer part of the first shell (6) is fixedly connected with a first connecting rod (7), the outer part of the first connecting rod (7) is fixedly connected with a heater (8), the inner side of the shell (1) is fixedly connected with a liquid conveying pipe (9), the outer part of the liquid conveying pipe (9) is movably connected with a rotating sleeve (10), the outer part of the rotating sleeve (10) is fixedly connected with a second rotating disc (11), the inner part of the second rotating disc (11) is fixedly connected with a second shell (, the outer portion of the second rotating disc (11) is movably connected with a bottom plate (15), the outer portion of the second rotating disc (11) is movably connected with a clamping block (16), the inner side of the shell (1) is movably connected with a second rotating shaft (17), the outer portion of the second rotating shaft (17) is fixedly connected with a second connecting rod (18), the outer portion of the second connecting rod (18) is movably connected with a first sliding block (19), the outer portion of the first sliding block (19) is movably connected with a third connecting rod (20), the inner side of the third connecting rod (20) is movably connected with a second sliding block (21), the outer portion of the second sliding block (21) is movably connected with a movable rod (22), the inner portion of the movable rod (22) is movably connected with a.

2. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: the infusion device is characterized in that the number of the movable blocks (3) is two, the specifications of the two movable blocks (3) are the same, the positions of the two movable blocks (3) are corresponding, a first spring is arranged between the two movable blocks (3), the positions of the two springs are corresponding, a first connecting rod (7) is provided with two connecting rods, the specifications of the two connecting rods (7) are the same, the positions of the two connecting rods are corresponding, a delivery pump is fixedly connected to the outside of an infusion tube (9), and a spray head is fixedly connected to the outside of the infusion.

3. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: four are no less than in heater (8), and four heaters (8) specification are the same and the position is corresponding, and the outside fixedly connected with connecting rod four of pivot one (2), and also fixedly connected with heater (8) in the outside of connecting rod four, the outside fixedly connected with motor one of pivot one (2), and pivot two (17) outside fixedly connected with motor two, motor one and motor two are all fixed connection in the outside of shell (1).

4. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: bottom plate (15) mainly comprise bottom plate one and bottom plate two, bottom plate one is no less than four and the equal angular distribution in the outside of carousel two (11), bottom plate two is no less than ten and the equal angular distribution in the outside of carousel two (11), the inside also fixedly connected with magnetic sheet (14) of bottom plate (15), magnetic sheet (14) and the inside magnetic sheet (14) position of casing two (12) of bottom plate (15) inside are corresponding, the outside fixedly connected with spring two of bottom plate (15), the one end fixed connection in the inside of carousel two (11) that bottom plate (15) were kept away from to spring two, the outside swing joint of slider three (24) has connecting rod five, the one end fixedly connected with electric chuck that slider three (24) was kept away from to connecting rod five.

5. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: the inside fixedly connected with electro-magnet of carousel II (11), the outside fixedly connected with spring III of electro-magnet, the one end fixed connection of electro-magnet is kept away from to spring III in the inside of iron plate, and iron plate fixed connection is in the inside of fixture block (16), and the specification of fixture block (16) and the specification phase-match of culture dish.

6. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: the magnetic plate (14) is movably connected with the lubricating oil (13), a spring IV is arranged inside the second shell (12), a first contact is arranged inside the second shell (12), a second contact is arranged outside the movable rod (22), the two contacts are two, and the two contacts have the same specification and are corresponding in position.

7. The device for culturing an experimental sample of biological cells, which is convenient for an experimenter to observe, according to claim 1, is characterized in that: the screw rod (23) and the rotating sleeve (10) are electrically connected with a driving power supply, the electric sucker, the conveying pump, the motor II, the motor I and the electromagnet are electrically connected with an external power supply, and the contact I, the contact II, the external power supply and the driving power supply are electrically connected with the control center.