CN112239500A - Antibody against novel coronavirus and kit for detecting novel coronavirus - Google Patents
Antibody against novel coronavirus and kit for detecting novel coronavirus Download PDFInfo
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- CN112239500A CN112239500A CN202011179251.2A CN202011179251A CN112239500A CN 112239500 A CN112239500 A CN 112239500A CN 202011179251 A CN202011179251 A CN 202011179251A CN 112239500 A CN112239500 A CN 112239500A
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Abstract
The invention discloses an antibody for resisting novel coronavirus and a kit for detecting the novel coronavirus, and relates to the technical field of antibodies. The antibodies against the novel coronavirus disclosed herein comprise a heavy chain complementarity determining region and a light chain complementarity determining region. The antibody has good affinity to the N protein of the novel coronavirus, and the detection of the novel coronavirus by using the antibody has good sensitivity and specificity. The invention provides more abundant antibody selection for the detection of the novel coronavirus.
Description
Technical Field
The invention relates to the technical field of antibodies, in particular to an antibody for resisting novel coronavirus and a kit for detecting the novel coronavirus.
Background
The structural proteins of the novel coronavirus 2019-nCoV are divided into: spike glycoprotein (S protein), envelope glycoprotein (E protein), membrane glycoprotein (M protein), and nucleocapsid protein (N protein), which include multiple epitopes. The N protein and the virus genome RNA are intertwined to form the virus nucleocapsid, which plays an important role in the synthesis process of the virus RNA. Meanwhile, the N protein is relatively conserved and accounts for the largest proportion in the structural proteins of the virus, and an organism can generate high-level antibodies for resisting the N protein in the early infection stage. Finally, the N protein is an important marker protein of the novel coronavirus, and the existence of the antigen can be detected through the N protein monoclonal antibody by utilizing the principle that the antigen is specifically combined with the antibody, so that the fact that the sample contains the novel coronavirus is directly proved, and the detection of the novel coronavirus is realized.
The detected antibodies are mainly divided into IgM and IgG. There is currently no systematic study of the production and duration of these two classes of antibodies to the novel coronaviruses. Usually, IgM antibody is produced early, is produced rapidly once infected, is maintained for a short time, disappears rapidly, and detection of positive in blood can reflect that the organism is in an acute infection state, and can be used as an indicator of early infection. Compared with the nucleic acid detection method, the antibody detection sample is serum or plasma, is less influenced by sample sampling, is beneficial to early diagnosis and removal of suspicious cases, and is quick and convenient to detect and suitable for large-scale screening.
The new coronavirus 2019-nCoV pneumonia epidemic situation spreads all over the world and seriously threatens the life safety and the body health of human beings. Respiratory droplets and close contact transmission are the main transmission routes of the novel coronavirus pneumonia, and the possibility of aerosol transmission exists in the case of long-term exposure to high-concentration aerosols in relatively closed environments. 2019-nCoV has strong infectivity, and clinical symptoms appear after infection of most patients, but some patients are asymptomatic infectors. After people are infected with coronavirus, the common signs of the person are respiratory symptoms, fever, cough, shortness of breath, dyspnea and the like. In more severe cases, the infection can lead to pneumonia, severe acute respiratory syndrome, renal failure, and even death. Although no specific treatment method exists for diseases caused by the novel coronavirus, the cure rate of patients with mild diseases or asymptomatic diseases can be greatly improved and the treatment time can be shortened by treating the patients with mild diseases or asymptomatic diseases. Therefore, the detection or identification of the patient becomes particularly important.
At present, nucleic acid detection and virus gene sequencing are mainly taken as etiological definite diagnosis evidence, and due to the influence of various factors such as sampling, operation, reagents and the like, false negative results can occur in nucleic acid detection. The positive detection rate of the virus nucleic acid of a suspected 2019 novel coronavirus (2019-nCoV) infected patient is only 30-50%. In addition, the nucleic acid detection has high requirements on instruments, equipment, detection sites and environmental conditions, has the defects of long detection time, low flux and the like, and is inconvenient for large-scale detection of people in the current epidemic situation. Therefore, there is an urgent need to develop a rapid and convenient detection kit for clinical detection, so as to isolate infected people as soon as possible to block virus transmission. Therefore, antibody detection kits are becoming more important.
At present, the 2019-nCoV N protein monoclonal antibody products are fewer, and the performances are different.
In view of this, the invention is particularly proposed.
Disclosure of Invention
The invention aims to provide an antibody for resisting novel coronavirus and a kit for detecting novel coronavirus, wherein the antibody can be specifically combined with N protein of the novel coronavirus, has higher affinity to the N protein, and has better sensitivity and specificity when used for detecting the novel coronavirus. The invention provides more abundant antibody selection for the detection of the novel coronavirus.
The invention is realized by the following steps:
in one aspect, the present invention provides an antibody or functional fragment thereof against a novel coronavirus or N protein thereof, said antibody or functional fragment thereof having the following complementarity determining regions:
CDR-VH 1: G-X1-T-F-T-X2-Y-X3-M-N; wherein: x1 is Y or F; x2 is D or N; x3 is A or G;
CDR-VH 2: W-X1-N-T-Y-X2-G-E-P-T-Y-A-X3-X4-F; wherein: x1 is L or I; x2 is T or S; x3 is D or E; x4 is D or E;
CDR-VH 3: A-R-X1-A-X2-X3-R-S-Y; wherein: x1 is S or T; x2 is I or L; x3 is I or L;
CDR-VL 1: K-A-S-X1-D-X2-S-T-A-X3-A; wherein: x1 is Q or E; x2 is I, V or L; x3 is I, V or L;
CDR-VL 2: W-X1-S-T-R-H-X2; wherein: x1 is A or G; x2 is T or S;
CDR-VL 3: Q-Q-H-X1-S-T-P-X2; wherein: x1 is Y or W; x2 is L, V or I.
The antibody or the functional fragment thereof for resisting the novel coronavirus, provided by the invention, has the complementarity determining region structure, and the complementarity determining region structure can ensure that the antibody or the functional fragment thereof can be specifically combined with the N protein for resisting the novel coronavirus, has better affinity for the N protein, and has better specificity and sensitivity when the antibody or the functional fragment thereof is used for detecting the novel coronavirus. The invention provides more abundant antibody selection for the detection of the novel coronavirus.
In alternative embodiments, in CDR-VH1, X1 is Y; in CDR-VH2, X1 is I; in CDR-VH3, X1 is S; in CDR-VL1, X1 is Q; in CDR-VL2, X1 is A.
The experimental results of this example show that the antibody exhibits higher affinity against the N protein of the novel coronavirus when the above mutation site in each complementarity determining region is the above amino acid residue.
In an alternative embodiment, in CDR-VH1, X2 is D.
In an alternative embodiment, in CDR-VH1, X2 is N.
In an alternative embodiment, in CDR-VH1, X3 is a.
In an alternative embodiment, in CDR-VH1, X3 is G.
In an alternative embodiment, in CDR-VH2, X2 is T.
In an alternative embodiment, in CDR-VH2, X2 is S.
In an alternative embodiment, in CDR-VH2, X3 is D.
In an alternative embodiment, in CDR-VH2, X3 is E.
In an alternative embodiment, in CDR-VH2, X4 is D.
In an alternative embodiment, in CDR-VH2, X4 is E.
In an alternative embodiment, in CDR-VH3, X2 is I.
In an alternative embodiment, in CDR-VH3, X2 is L.
In an alternative embodiment, in CDR-VH3, X3 is I.
In an alternative embodiment, in CDR-VH3, X3 is L.
In an alternative embodiment, in CDR-VL1, X2 is I.
In an alternative embodiment, in CDR-VL1, X2 is V.
In an alternative embodiment, in CDR-VL1, X2 is L.
In an alternative embodiment, in CDR-VL1, X3 is I.
In an alternative embodiment, in CDR-VL1, X3 is V.
In an alternative embodiment, in CDR-VL1, X3 is L.
In an alternative embodiment, in CDR-VL2, X2 is T.
In an alternative embodiment, in CDR-VL2, X2 is S.
In an alternative embodiment, in CDR-VL3, X1 is Y.
In an alternative embodiment, in CDR-VL3, X1 is W.
In an alternative embodiment, in CDR-VL3, X2 is L.
In an alternative embodiment, in CDR-VL3, X2 is V.
In an alternative embodiment, in CDR-VL3, X2 is I.
In alternative embodiments, each complementarity determining region of the antibody, or functional fragment thereof, is selected from any one of the following combinations of mutations 1-42:
in an alternative embodiment, the antibody or functional fragment thereof binds to the N protein of the anti-novel coronavirus with KD≤8×10-9Affinity binding in mol/L.
In an alternative embodiment, KD≤4×10-10mol/L。
In an alternative embodiment, KD≤7×10-9mol/L、KD≤6×10-9mol/L、KD≤5×10-9mol/L、KD≤4×10-9mol/L、KD≤3×10-9mol/L、KD≤2×10-9mol/L、KD≤1×10-9mol/L、KD≤9×10-10mol/L、KD≤8×10-10mol/L、KD≤7×10-10mol/L、KD≤6×10-10mol/L、KD≤5×10-10mol/L、KD≤4×10-10mol/L、KD≤3×10-10mol/L、KD≤2×10-10mol/L、KD≤1×10-10mol/L、KD≤9×10-11mol/L、KD≤8×10-11mol/L or KD≤7×10-11mol/L。
In an alternative embodiment, 7.54 × 10-11mol/L≤KD≤3.28×10-10mol/L。
KDThe detection of (2) is carried out with reference to the method in the examples of the present invention.
In alternative embodiments, in CDR-VH1, X1 is F; in CDR-VH2, X1 is L; in CDR-VH3, X1 is T; in CDR-VL1, X1 is E; in CDR-VL2, X1 is G.
In alternative embodiments, each complementarity determining region of the antibody or functional fragment thereof is selected from any one of the following combinations of mutations 43-48:
in alternative embodiments, the antibody comprises the light chain framework regions FR1-L, FR2-L, FR3-L and FR4-L in sequence as set forth in SEQ ID NOS: 1-4, and/or the heavy chain framework regions FR1-H, FR2-H, FR3-H and FR4-H in sequence as set forth in SEQ ID NOS: 5-8.
In general, the variable regions of the heavy chain (VH) and light chain (VL) can be obtained by linking the CDRs and FRs numbered below in a combined arrangement as follows: FR1-CDR1-FR2-CDR2-FR3-CDR3-FR 4.
It is noted that in other embodiments, each framework region amino acid sequence of an antibody or functional fragment thereof provided herein can have at least 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% homology to the corresponding framework region described above (SEQ ID NO:1, 2, 3, 4, 5, 6, 7, or 8).
In alternative embodiments, the antibody further comprises a constant region.
In alternative embodiments, the constant region is selected from the constant regions of any one of IgG1, IgG2, IgG3, IgG4, IgA, IgM, IgE, and IgD.
In alternative embodiments, the species of the constant region is derived from a cow, horse, dairy cow, pig, sheep, goat, rat, mouse, dog, cat, rabbit, camel, donkey, deer, mink, chicken, duck, goose, turkey, chicken fountains, or human.
In alternative embodiments, the constant region is derived from a mouse.
In alternative embodiments, the light chain constant region sequence of the constant region is set forth in SEQ ID NO. 9 and the heavy chain constant region sequence of the constant region is set forth in SEQ ID NO. 10.
In alternative embodiments, the functional fragment is selected from any one of VHH, F (ab ') 2, Fab', Fab, Fv and scFv of the antibody.
Functional fragments of the above antibodies typically have the same binding specificity as the antibody from which they are derived. It will be readily understood by those skilled in the art from the disclosure of the present invention that functional fragments of the above antibodies can be obtained by methods such as enzymatic digestion (including pepsin or papain) and/or by chemical reduction cleavage of disulfide bonds. Based on the disclosure of the structure of the intact antibody, the above-described functional fragments are readily available to those skilled in the art.
Functional fragments of the above antibodies can also be obtained by recombinant genetic techniques also known to those skilled in the art or synthesized by, for example, automated peptide synthesizers, such as those sold by Applied BioSystems and the like.
In another aspect, the present invention provides a reagent or a kit for detecting a novel coronavirus or an N protein thereof, comprising the antibody or the functional fragment thereof as described in any one of the above.
In an alternative embodiment, the antibody or functional fragment thereof in the above-described reagent or kit is labeled with a detectable label.
Detectable labels are substances having properties, such as luminescence, color development, radioactivity, etc., which can be observed directly by the naked eye or detected by an instrument, by which qualitative or quantitative detection of the respective target substance can be achieved.
In alternative embodiments, the detectable labels include, but are not limited to, fluorescent dyes, enzymes that catalyze the development of a substrate, radioisotopes, chemiluminescent reagents, and nanoparticle-based labels.
In the actual use process, one skilled in the art can select a suitable marker according to the detection condition or actual requirement, and whatever marker is used belongs to the protection scope of the present invention.
In alternative embodiments, the fluorescent dyes include, but are not limited to, fluorescein-based dyes and derivatives thereof (e.g., including, but not limited to, Fluorescein Isothiocyanate (FITC) hydroxyphoton (FAM), tetrachlorofluorescein (TET), etc. or analogs thereof), rhodamine-based dyes and derivatives thereof (e.g., including, but not limited to, red Rhodamine (RBITC), Tetramethylrhodamine (TAMRA), rhodamine b (tritc), etc. or analogs thereof), Cy-series dyes and derivatives thereof (e.g., including, but not limited to, Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, Cy3, etc. or analogs thereof), Alexa-series dyes and derivatives thereof (e.g., including, but not limited to, Alexa fluor350, 405, 430, 488, 532, 546, 555, 568, 594, 610, 33, 647, 680, 700, 750, etc. or analogs thereof), and protein-based dyes and derivatives thereof (e.g., including, but not limited to, Phycoerythrin (PE), phycocyanin (PC, Allophycocyanin (APC), polymethacrylic flavin-chlorophyll protein (precP), etc.).
In alternative embodiments, the enzyme that catalyzes the color development of the substrate includes, but is not limited to, horseradish peroxidase, alkaline phosphatase, beta-galactosidase, glucose oxidase, carbonic anhydrase, acetylcholinesterase, and glucose-6-phosphate deoxyenzyme.
In alternative embodiments, the radioisotope includes, but is not limited to212Bi、131I、111In、90Y、186Re、211At、125I、188Re、153Sm、213Bi、32P、94mTc、99mTc、203Pb、67Ga、68Ga、43Sc、47Sc、110mIn、97Ru、62Cu、64Cu、67Cu、68Cu、86Y、88Y、121Sn、161Tb、166Ho、105Rh、177Lu、172Lu and18F。
in alternative embodiments, the chemiluminescent reagent includes, but is not limited to, luminol and its derivatives, lucigenin, crustacean fluorescein and its derivatives, bipyridyl ruthenium and its derivatives, acridinium ester and its derivatives, dioxane and its derivatives, lotrine and its derivatives, and peroxyoxalate and its derivatives.
In alternative embodiments, the nanoparticle-based labels include, but are not limited to, nanoparticles, colloids, organic nanoparticles, magnetic nanoparticles, quantum dot nanoparticles, and rare earth complex nanoparticles.
In alternative embodiments, the colloid includes, but is not limited to, colloidal metals, disperse dyes, dye-labeled microspheres, and latex.
In alternative embodiments, the colloidal metals include, but are not limited to, colloidal gold, colloidal silver, and colloidal selenium.
In another aspect, the present invention provides a nucleic acid molecule encoding the above antibody or functional fragment thereof.
In another aspect, the invention provides a vector comprising the nucleic acid molecule described above.
In another aspect, the present invention provides a recombinant cell comprising the vector described above.
In another aspect, the present invention provides a method of preparing an antibody or functional fragment thereof, comprising: culturing the recombinant cell as described above, and separating and purifying the antibody or functional fragment thereof from the culture product.
Based on the disclosure of the amino acid sequence of the antibody or its functional fragment, it is easy for those skilled in the art to think that the antibody or its functional fragment can be prepared by genetic engineering techniques or other techniques (chemical synthesis, hybridoma cells), for example, by separating and purifying the antibody or its functional fragment from the culture product of recombinant cells capable of recombinantly expressing the antibody or its functional fragment as described above, and this is within the scope of the present invention, regardless of the technique used to prepare the antibody or its functional fragment.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 shows the result of reducing SDS-PAGE of the anti-novel coronavirus antibody of example 1.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the formulations or unit dosages herein, some are now described. Unless otherwise indicated, the techniques employed or contemplated herein are standard methods. The materials, methods, and examples are illustrative only and not intended to be limiting.
The practice of the present invention will employ, unless otherwise indicated, conventional techniques of cell biology, molecular biology (including recombinant techniques), microbiology, biochemistry and immunology, which are within the skill of the art. Such techniques are well explained in the literature, e.g. "molecular cloning: a Laboratory Manual, second edition (Sambrook et al, 1989); oligonucleotide Synthesis (oligo Synthesis) (eds. m.j. goal, 1984); animal Cell Culture (Animal Cell Culture), ed.r.i. freshney, 1987; methods in Enzymology (Methods in Enzymology), Handbook of Experimental Immunology (Handbook of Experimental Immunology) (ed. D.M.Weir and C.C.Black well), Gene Transfer Vectors for Mammalian Cells (ed. J.M.Miller and M.P.Calos) (ed. J.M.and M.P.Calos) (ed. 1987), Methods in Current Generation (Current Protocols in Molecular Biology) (ed. F.M.Ausubel.et al, 1987), PCR, Polymerase Chain Reaction (ed. PCR: The Polymerase Chain Reaction) (ed. Mullis et al, 1994), and Methods in Current Immunology (ed. J.1991).
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
Restriction enzyme, Prime Star DNA polymerase, was purchased from Takara in this example. MagExtractor-RNA extraction kit was purchased from TOYOBO. BD SMARTTMRACE cDNA Amplification Kit was purchased from Takara. pMD-18T vector was purchased from Takara. Plasmid extraction kits were purchased from Tiangen corporation. Primer synthesis and gene sequencing were performed by Invitrogen corporation.
1 construction of recombinant plasmid
(1) Antibody Gene preparation
mRNA is extracted from a hybridoma cell strain secreting an antibody against a novel coronavirus N protein, a DNA product is obtained by an RT-PCR method, the product is added with A by rTaq DNA polymerase for reaction and then inserted into a pMD-18T vector, the product is transformed into DH5 alpha competent cells, the Heavy Chain and Light Chain genes are respectively taken for cloning after a colony is grown, and each 4 clones are sent to a gene sequencing company for sequencing.
(2) Sequence analysis of antibody variable region genes
Putting the gene sequence obtained by sequencing in an IMGT antibody database for analysis, and analyzing by using VNTI11.5 software to determine that the genes amplified by the heavy Chain primer pair and the Light Chain primer pair are correct, wherein in the gene fragment amplified by the Light Chain, the VL gene sequence is 324bp, belongs to VkII gene family, and a leader peptide sequence of 57bp is arranged in front of the VL gene sequence; in the gene fragment amplified by the Heavy Chain primer pair, the VH gene sequence is 357bp, belongs to a VH1 gene family, and has a leader peptide sequence of 57bp in front.
(3) Construction of recombinant antibody expression plasmid
pcDNATM3.4vector is a constructed recombinant antibody eukaryotic expression vector, and multiple cloning enzyme cutting sites such as HindIII, BamHI, EcoRI and the like are introduced into the expression vector and named as pcDNA3.4A expression vector, and the vector is called as 3.4A expression vector for short in the following; according to the sequencing result of the antibody variable region gene in the pMD-18T, VL and VH gene specific primers of the antibody are designed, two ends of the primers are respectively provided with HindIII and EcoRI restriction sites and protective bases, and a Light Chain gene fragment of 0.73kb and a Heavy Chain gene fragment of 1.42kb are amplified by a PCR amplification method.
The gene fragments of the Heavy Chain and the Light Chain are subjected to double enzyme digestion by HindIII/EcoRI respectively, the 3.4A vector is subjected to double enzyme digestion by HindIII/EcoRI, the Heavy Chain gene and the Light Chain gene are respectively connected into the 3.4A expression vector after the fragments and the vector are purified and recovered, and recombinant expression plasmids of the Heavy Chain and the Light Chain are respectively obtained.
2 Stable cell line selection
(1) Transient transfection of recombinant antibody expression plasmid into CHO cells and determination of expression plasmid activity
Plasmid was diluted to 400ng/ml with ultrapure water and CHO cells were conditioned at 1.43X 107cells/ml are put into a centrifuge tube, 100 mu L of plasmid is mixed with 700 mu L of cells, the mixture is transferred into an electric rotating cup and is electrically rotated, the sampling counting is carried out on days 3, 5 and 7, and the sampling detection is carried out on day 7.
Diluting 2019-nCoV N protein antigen to 1 mu g/ml by using the coating solution, wherein each well is 100 mu L, and the temperature is kept overnight at 4 ℃; the next day, washing with the washing solution for 2 times, and patting dry; adding blocking solution (20% BSA + 80% PBS), and drying at 37 deg.C for 1 hr in each well; adding diluted cell supernatant at 100 μ L/well, 37 deg.C for 30min (partial supernatant for 1 h); washing with washing solution for 5 times, and drying; adding goat anti-mouse IgG-HRP (goat anti-mouse IgG-HRP) with the concentration of 100 mu L per well at 37 ℃ for 30 min; washing with washing solution for 5 times, and drying; adding a developing solution A (50 μ L/hole, containing citric acid + sodium acetate + acetanilide + carbamide peroxide), and adding a developing solution B (50 μ L/hole, containing citric acid + EDTA-2 Na + TM)B + concentrated HCl), 10 min; adding stop solution (50 μ L/well, EDTA-2 Na + concentrated H)2SO4) (ii) a OD readings were taken at 450nm (reference 630nm) on the microplate reader. The results show that the reaction OD after the cell supernatant is diluted 1000 times is still larger than 1.0, and the reaction OD of the wells without the cell supernatant is smaller than 0.1, which indicates that the antibody generated after the plasmid is transiently transformed has activity on the 2019-nCoV N protein antigen.
(2) Linearization of recombinant antibody expression plasmids
The following reagents were prepared: buffer 50 mu L, DNA 100 mu g/tube, Puv I enzyme 10 mu L, sterile water to 500 mu L, 37 ℃ water bath enzyme digestion overnight; sequentially extracting with equal volume of phenol/chloroform/isoamyl alcohol (lower layer) 25:24:1 and then chloroform (water phase); precipitating with 0.1 volume (water phase) of 3M sodium acetate and 2 volumes of ethanol on ice, rinsing with 70% ethanol, removing organic solvent, re-melting with appropriate amount of sterilized water after ethanol is completely volatilized, and finally measuring concentration.
(3) Stable transfection of recombinant antibody expression plasmid, pressurized screening of stable cell lines
Plasmid was diluted to 400ng/ml with ultrapure water and CHO cells were conditioned at 1.43X 107cells/ml are put into a centrifuge tube, 100 mu L of plasmid is mixed with 700 mu L of cells, and the mixture is transferred into an electric rotating cup and is electrically rotated, and the next day is counted; 25umol/L MSX 96-well pressure culture for about 25 days.
Observing the marked clone holes with cells under a microscope, and recording the confluence degree; taking culture supernatant, and sending the culture supernatant to a sample for detection; selecting cell strains with high antibody concentration and relative concentration, transferring the cell strains into 24 holes, and transferring the cell strains into 6 holes after 3 days; after 3 days, the seeds were kept and cultured in batches, and the cell density was adjusted to 0.5X 106cells/ml, 2.2ml, cell density 0.3X 106cell/ml, 2ml for seed preservation; and (4) 7 days, carrying out batch culture supernatant sample sending detection in 6 holes, and selecting cell strains with small antibody concentration and cell diameter to transfer TPP for seed preservation and passage.
3 recombinant antibody production
(1) Cell expanding culture
After the cells are recovered, the cells are cultured in a shaking flask with the specification of 125ml, the inoculation volume is 30ml, the culture medium is 100% Dynamis culture medium, and the cells are placed in a shaking table with the rotation speed of 120r/min, the temperature of 37 ℃ and the carbon dioxide of 8%. Culturing for 72h, inoculating and expanding culture at an inoculation density of 50 ten thousand cells/ml, wherein the expanding culture volume is calculated according to production requirements, and the culture medium is 100% Dynamis culture medium. Then the culture is expanded every 72 h. When the cell amount meets the production requirement, the production is carried out by strictly controlling the inoculation density to be about 50 ten thousand cells/ml.
(2) Shake flask production and purification
Shake flask parameters: the rotating speed is 120r/min, the temperature is 37 ℃, and the carbon dioxide is 8 percent. Feeding in a flowing mode: daily feeding was started when the culture was carried out for 72h in a shake flask, 3% of the initial culture volume was fed daily to HyCloneTM Cell BoostTM Feed 7a, and one thousandth of the initial culture volume was fed daily to Feed 7b, up to day 12 (day 12 feeding). Glucose was supplemented with 3g/L on the sixth day. Samples were collected on day 13. Affinity purification was performed using a proteinA affinity column. Mu.g of the purified antibody was subjected to reducing SDS-PAGE, and 4. mu.g of an external control antibody was used as a control, and the electrophoretogram showed two bands, 1 of which Mr was 50KD (heavy chain, SEQ ID NO:14) and the other Mr was 28KD (light chain, SEQ ID NO:13), as shown in FIG. 1 below, after the reducing SDS-PAGE.
Example 2
Detection of antibody Performance
(1) Example 1 Activity assay of antibodies and mutants thereof
Analysis of the antibody (WT) sequence of example 1 showed that the heavy chain variable region is represented by SEQ ID NO:12, wherein the amino acid sequence of each complementarity determining region in the heavy chain variable region is as follows:
CDR-VH1:G-F(X1)-T-F-T-D(X2)-Y-A(X3)-M-N;
CDR-VH2:W-L(X1)-N-T-Y-S(X2)-G-E-P-T-Y-A-E(X3)-D(X4)-F;
CDR-VH3:A-R-T(X1)-A-I(X2)-L(X3)-R-S-Y;
the light chain variable region is shown as SEQ ID NO:11, wherein the amino acid sequences of the complementarity determining regions on the light chain variable region are as follows:
CDR1-VL:K-A-S-E(X1)-D-L(X2)-S-T-A-L(X3)-A;
CDR-VL2:W-G(X1)-S-T-R-H-S(X2);
CDR-VL3:Q-Q-H-W(X1)-S-T-P-L(X2)。
based on the anti-novel coronavirus antibody (WT) of example 1, mutations were made in the complementarity determining regions at sites involved in the activity of the antibody, wherein X1, X2, X3, and X4 were all mutated sites. See table 1 below.
TABLE 1 mutant sites associated with antibody Activity
Antibody binding activity assay in table 1:
coating liquid (main component NaHCO)3) Diluting 2019-nCoV N protein antigen to 1 mu g/ml for coating a microplate, wherein each well is 100 mu l, and the temperature is kept overnight at 4 ℃; the next day, washing with the washing solution for 2 times, and patting dry; adding blocking solution (20% BSA + 80% PBS), beating to dry at 37 deg.C for 1 hr, and adding blocking solution (120 μ l per well); adding the diluted monoclonal antibody in the table 1, 100 mul/hole, 37 ℃, 30min-60 min; washing with washing solution for 5 times, and drying; adding goat anti-mouse IgG-HRP (goat anti-mouse IgG-HRP) with the concentration of 100 mu l per well at 37 ℃ for 30 min; washing with washing solution (PBS) for 5 times, and drying; adding color development liquid A (50 μ L/well containing 2.1g/L citric acid, 12.25g/L citric acid, 0.07g/L acetanilide and 0.5g/L carbamide peroxide) and adding color development liquid B (50 μ L/well containing 1.05g/L citric acid, 0.186g/L LEDTA.2Na, 0.45g/L TMB and 0.2ml/L concentrated HCl) for 10 min; stop solution (50. mu.l/well, containing 0.75 g/EDTA-2 Na and 10.2ml/L concentrated H) was added2SO4) (ii) a OD readings were taken at 450nm (reference 630nm) on the microplate reader. The results are shown in Table 2 below.
TABLE 2 Activity data of WT antibodies and mutants thereof
Antibody concentration (ng/ml) | 5000 | 1000 | 500 | 250 | 125 | 0.00 |
WT | 0.712 | 0.731 | 0.351 | 0.152 | 0.52 | 0.090 |
Mutation 1 | 0.952 | 0.825 | 0.625 | 0.364 | 0.204 | 0.025 |
Mutation 2 | 0.912 | 0.831 | 0.631 | 0.402 | 0.214 | 0.028 |
Mutation 3 | 0.825 | 0.841 | 0.594 | 0.387 | 0.198 | 0.022 |
Mutation 4 | 0.847 | 0.918 | 0.542 | 0.362 | 0.148 | 0.023 |
Mutation 5 | 0.168 | 0.021 | - | - | - | - |
Mutation 6 | 0.146 | 0.02 | - | - | - | - |
The data in table 2 show that WT, mutant 1 to mutant 4 antibodies are more active against the N protein antigen and that mutant 5 and mutant 6 are less active; among them, mutation 1 had the highest activity.
(2) Affinity detection of antibodies and mutants thereof
(a) Based on mutation 1, other sites were mutated, and the sequence of each mutation is shown in table 3 below.
TABLE 3 mutation sites related to antibody affinity
Affinity assay
Using AMC sensors, the purified antibody was diluted to 10. mu.g/mL with PBST, and the 2019-nCoV N protein antigen was diluted with PBST in a gradient: 1.41. mu.g/mL, 0.70. mu.g/mL, 0.35. mu.g/mL, 0.18. mu.g/mL, 0.09. mu.g/mL, 0.04. mu.g/mL.
The operation flow is as follows: equilibrating in buffer 1(PBST) for 60s, immobilizing antibody in antibody solution for 300s, incubating in buffer 2(PBST) for 180s, binding in antigen solution for 420s, dissociating in buffer 2 for 1200s, regenerating the sensor with 10mM GLY solution pH 1.69 and buffer 3, and outputting the data. KDRepresents the equilibrium dissociation constant, i.e., affinity; kon denotes the binding rate; kdis denotes the off-rate. The results are shown in Table 4 below.
Table 4 affinity assay data
The data in table 4 show that both mutation 1 and its mutant have higher affinity for the N protein antigen, indicating that all antibodies obtained by mutation in the manner of table 3 based on mutation 1 have higher affinity.
(b) Based on WT, mutation is carried out on other sites, and the affinity of each mutant is detected, the sequence of each mutation is shown in Table 5, and the corresponding affinity data is shown in Table 6.
TABLE 5 mutations with WT as backbone
TABLE 6 affinity assay results for WT antibodies and their mutants
The data in Table 6 show that WT and its mutants also have better affinity for the N protein antigen, indicating that all antibodies mutated in the manner shown in Table 5 have better affinity based on WT.
(3) Evaluation of stability against naked antibody
Placing the antibody in a temperature range of 4 ℃ (refrigerator), -80 ℃ (refrigerator) and 37 ℃ (thermostat) for 21 days, taking samples in 7 days, 14 days and 21 days for state observation, and performing activity detection on the samples in 21 days, wherein the result shows that under three examination conditions, no obvious protein state change is seen in 21 days of placing the antibody, and the activity does not show a descending trend along with the rise of the examination temperature, which indicates that the antibody is stable. The following table 7 shows the results of the detection of OD by the antibody of mutant 1 in the enzyme immunity activity test for 21 days.
TABLE 7
Sample concentration (ng/ml) | 500 | 250 | 0 |
Samples at 4 ℃ for 21 days | 0.708 | 0.414 | 0.034 |
21 days samples at-80 deg.C | 0.752 | 0.521 | 0.032 |
21 day samples at 37 deg.C | 0.731 | 0.551 | 0.037 |
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Sequence listing
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Claims (10)
1. An antibody or functional fragment thereof against a novel coronavirus or its N protein, wherein said antibody or functional fragment thereof comprises the following complementarity determining regions:
CDR-VH 1: G-X1-T-F-T-X2-Y-X3-M-N; wherein: x1 is Y or F; x2 is D or N; x3 is A or G;
CDR-VH 2: W-X1-N-T-Y-X2-G-E-P-T-Y-A-X3-X4-F; wherein: x1 is L or I; x2 is T or S; x3 is D or E; x4 is D or E;
CDR-VH 3: A-R-X1-A-X2-X3-R-S-Y; wherein: x1 is S or T; x2 is I or L; x3 is I or L;
CDR-VL 1: K-A-S-X1-D-X2-S-T-A-X3-A; wherein: x1 is Q or E; x2 is I, V or L; x3 is I, V or L;
CDR-VL 2: W-X1-S-T-R-H-X2; wherein: x1 is A or G; x2 is T or S;
CDR-VL 3: Q-Q-H-X1-S-T-P-X2; wherein: x1 is Y or W; x2 is L, V or I.
2. The antibody or functional fragment thereof according to claim 1,
in CDR-VH1, X1 is Y;
in CDR-VH2, X1 is I;
in CDR-VH3, X1 is S;
in CDR-VL1, X1 is Q;
in CDR-VL2, X1 is A;
preferably, in CDR-VH1, X2 is D;
preferably, in CDR-VH1, X2 is N;
preferably, in CDR-VH1, X3 is A;
preferably, in CDR-VH1, X3 is G;
preferably, in CDR-VH2, X2 is T;
preferably, in CDR-VH2, X2 is S;
preferably, in CDR-VH2, X3 is D;
preferably, in CDR-VH2, X3 is E;
preferably, in CDR-VH2, X4 is D;
preferably, in CDR-VH2, X4 is E;
preferably, in CDR-VH3, X2 is I;
preferably, in CDR-VH3, X2 is L;
preferably, in CDR-VH3, X3 is I;
preferably, in CDR-VH3, X3 is L;
preferably, in CDR-VL1, X2 is I;
preferably, in CDR-VL1, X2 is V;
preferably, in CDR-VL1, X2 is L;
preferably, in CDR-VL1, X3 is I;
preferably, in CDR-VL1, X3 is V;
preferably, in CDR-VL1, X3 is L;
preferably, in CDR-VL2, X2 is T;
preferably, in CDR-VL2, X2 is S;
preferably, in CDR-VL3, X1 is Y;
preferably, in CDR-VL3, X1 is W;
preferably, in CDR-VL3, X2 is L;
preferably, in CDR-VL3, X2 is V;
preferably, in CDR-VL3, X2 is I;
preferably, each complementarity determining region of the antibody or functional fragment thereof is selected from any one of the following combinations of mutations 1-42:
3. the antibody or functional fragment thereof according to claim 2, wherein the antibody or functional fragment thereof binds to the N protein of a novel coronavirus with KD≤8×10-9Affinity binding of mol/L; preferably, KD≤4×10-10mol/L。
4. The antibody or functional fragment thereof according to claim 1,
in CDR-VH1, X1 is F;
in CDR-VH2, X1 is L;
in CDR-VH3, X1 is T;
in CDR-VL1, X1 is E;
in CDR-VL2, X1 is G;
preferably, each complementarity determining region of the antibody or functional fragment thereof is selected from any one of the following combinations of mutations 43-48:
5. the antibody or functional fragment thereof according to any one of claims 1 to 4, wherein said antibody comprises the light chain framework regions FR1-L, FR2-L, FR3-L and FR4-L in sequence as set forth in SEQ ID Nos. 1-4, and/or the heavy chain framework regions FR1-H, FR2-H, FR3-H and FR4-H in sequence as set forth in SEQ ID Nos. 5-8;
preferably, the antibody further comprises a constant region;
preferably, the constant region is selected from the constant regions of any one of IgG1, IgG2, IgG3, IgG4, IgA, IgM, IgE and IgD;
preferably, the species of the constant region is from a cow, horse, cow, pig, sheep, goat, rat, mouse, dog, cat, rabbit, camel, donkey, deer, mink, chicken, duck, goose, turkey, chicken fight, or human;
preferably, the constant region is derived from a mouse;
preferably, the light chain constant region sequence of the constant region is shown as SEQ ID NO. 9, and the heavy chain constant region sequence of the constant region is shown as SEQ ID NO. 10;
preferably, the functional fragment is selected from any one of VHH, F (ab ') 2, Fab', Fab, Fv and scFv of the antibody.
6. A reagent or kit for detecting a novel coronavirus or N protein thereof, comprising the antibody or functional fragment thereof according to any one of claims 1 to 5.
7. The reagent or kit according to claim 6, wherein the antibody or functional fragment thereof is labeled with a detectable label;
preferably, the detectable label is selected from the group consisting of a fluorescent dye, an enzyme that catalyzes the color development of a substrate, a radioisotope, a chemiluminescent reagent, and a nanoparticle-based label;
preferably, the fluorescent dye is selected from fluorescein dyes and derivatives thereof, rhodamine dyes and derivatives thereof, Cy dyes and derivatives thereof, Alexa dyes and derivatives thereof, and protein dyes and derivatives thereof;
preferably, the enzyme catalyzing the color development of the substrate is selected from the group consisting of horseradish peroxidase, alkaline phosphatase, beta-galactosidase, glucose oxidase, carbonic anhydrase, acetylcholinesterase, and glucose-6-phosphate deoxyenzyme;
preferably, the radioisotope is selected from the group consisting of212Bi、131I、111In、90Y、186Re、211At、125I、188Re、153Sm、213Bi、32P、94mTc、99mTc、203Pb、67Ga、68Ga、43Sc、47Sc、110mIn、97Ru、62Cu、64Cu、67Cu、68Cu、86Y、88Y、121Sn、161Tb、166Ho、105Rh、177Lu、172Lu and18F;
preferably, the chemiluminescent reagent is selected from luminol and its derivatives, lucigenin, crustacean fluorescein and its derivatives, bipyridine ruthenium and its derivatives, acridinium ester and its derivatives, dioxetane and its derivatives, lowhine and its derivatives, and peroxyoxalate and its derivatives;
preferably, the nanoparticle-based label is selected from the group consisting of nanoparticles, colloids, organic nanoparticles, magnetic nanoparticles, quantum dot nanoparticles, and rare earth complex nanoparticles;
preferably, the colloid is selected from the group consisting of colloidal metals, disperse dyes, dye-labeled microspheres, and latexes;
preferably, the colloidal metal is selected from the group consisting of colloidal gold, colloidal silver and colloidal selenium.
8. A vector comprising a nucleic acid fragment encoding the antibody or functional fragment thereof according to any one of claims 1 to 5.
9. A recombinant cell comprising the vector of claim 8.
10. A method for producing an antibody or functional fragment thereof according to any one of claims 1 to 5, comprising: culturing the recombinant cell of claim 9, and isolating and purifying the antibody or functional fragment thereof from the culture product.
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CN117659180A (en) * | 2022-09-06 | 2024-03-08 | 东莞市朋志生物科技有限公司 | Anti-novel coronavirus antibody or functional fragment thereof, reagent for detecting novel coronavirus and kit |
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CN112239500B (en) | 2022-01-07 |
KR20230093028A (en) | 2023-06-26 |
JP2023551102A (en) | 2023-12-07 |
WO2022089045A1 (en) | 2022-05-05 |
CA3200150A1 (en) | 2022-05-05 |
CN114426575A (en) | 2022-05-03 |
CN114426575B (en) | 2023-10-03 |
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