CN112226357A - Biological culture device and method - Google Patents
Biological culture device and method Download PDFInfo
- Publication number
- CN112226357A CN112226357A CN202011165232.4A CN202011165232A CN112226357A CN 112226357 A CN112226357 A CN 112226357A CN 202011165232 A CN202011165232 A CN 202011165232A CN 112226357 A CN112226357 A CN 112226357A
- Authority
- CN
- China
- Prior art keywords
- box body
- culture
- motor fan
- carbon dioxide
- fixedly connected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 12
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 70
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 35
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 35
- 244000005700 microbiome Species 0.000 claims abstract description 17
- 238000009423 ventilation Methods 0.000 claims description 18
- 238000007789 sealing Methods 0.000 claims description 13
- 238000005485 electric heating Methods 0.000 claims description 10
- 239000006199 nebulizer Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 230000006978 adaptation Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 13
- 239000001963 growth medium Substances 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229940099259 vaseline Drugs 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/42—Integrated assemblies, e.g. cassettes or cartridges
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a biological culture device and a method thereof, and relates to the technical field of biological culture. A biological culture device and a method thereof comprise a culture box body, wherein the top and the bottom of the culture box body are respectively provided with a first vent hole and a second vent hole, a first motor fan is fixedly installed inside the first vent hole, a second motor fan is fixedly installed inside the second vent hole, the top and the bottom of the culture box body are respectively and fixedly provided with a first electric valve and a second electric valve, the first electric valve is matched with the first vent hole, and the second electric valve is matched with the second vent hole. According to the invention, through the arrangement of the compressed gas tank, the ultrasonic atomizer, the first motor fan and the second motor fan, the carbon dioxide content and the humidity of the culture environment in the culture box body can be accurately controlled, so that a more suitable microorganism growth environment can be regulated, and the culture efficiency of microorganisms is improved.
Description
Technical Field
The invention relates to the technical field of biological culture, in particular to a biological culture device and a method thereof.
Background
Microbial culture, which refers to the rapid growth and propagation of certain microorganisms by means of artificially prepared culture media and artificially created culture conditions, is called microbial culture, and can be divided into pure culture and mixed culture, wherein the former refers to the culture and utilization of purified single strains; the latter means that the microorganisms in the mixed strains or natural samples are cultured, and then the diversity and the quantity of the microorganisms in the soil can be estimated to a certain extent according to the types and the quantity of the microorganisms growing on the culture medium.
The method for controlling the carbon dioxide content and the temperature and humidity in the growth environment of certain microorganisms during primary separation culture of the microorganisms is as follows: taking a covered ground specimen jar or a glass dryer, putting the inoculated culture medium into the jar, lighting a candle, then putting the jar at a position slightly higher than the culture, coating vaseline on the jar cover or the jar opening, covering and sealing the jar, gradually reducing the oxygen generated by candle combustion in the jar, automatically extinguishing the candle after several minutes, increasing the carbon dioxide content in the container, and putting the jar into a proper common incubator for incubation.
Disclosure of Invention
The present invention is directed to a biological culture apparatus and method thereof to solve the problems set forth in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: a biological culture device and a method thereof comprise a culture box body, wherein the top and the bottom of the culture box body are respectively provided with a first vent hole and a second vent hole, the inside of the first vent hole is fixedly provided with a first motor fan, the inside of the second vent hole is fixedly provided with a second motor fan, the top and the bottom of the culture box body are respectively fixedly provided with a first electric valve and a second electric valve, the first electric valve is matched with the first vent hole, the second electric valve is matched with the second vent hole, one side of the bottom of the culture box body is fixedly connected with a fixed hoop, the culture box body is fixedly connected with a compressed gas tank through the fixed hoop, one end of the compressed gas tank is fixedly connected with an exhaust pipe, the other end of the compressed gas tank is fixedly connected with an inflation pipe, the middle part of the exhaust pipe is fixedly provided with a first control valve, and the middle part of the inflation pipe is fixedly provided with a second, the one end of blast pipe runs through and cultivates the box and extends to its inside, the back fixed mounting who cultivates the box has ultrasonic nebulizer, and ultrasonic nebulizer's top fixedly connected with connecting pipe, the connecting pipe extends to the inside of cultivateing the box, cultivate the equal fixedly connected with fixed strip of inner wall of box both sides, and the top joint of fixed strip has the board of placing, the intermediate position difference fixed mounting who cultivates the box both sides has carbon dioxide sensor and humidity transducer, cultivate one side lateral wall fixed mounting at box top has temperature sensor, the roof fixed mounting who cultivates the box has the electric plate, and the electric plate is located first ventilation hole under.
Furthermore, the placing plate comprises a porous plate body, limiting strips are fixedly connected to two sides of the bottom of the porous plate body, ventilation holes are formed in the middle of the porous plate body and are evenly distributed, a limiting groove is formed in the top of the fixing strip, and the limiting groove is matched with the limiting strips.
Further, cultivate the equal fixedly connected with universal wheel in four corners of bottom half, the top of universal wheel is provided with the braking piece, cultivate the inside one side fixedly connected with second hinge of box, and cultivate the box and pass through second hinge fixedly connected with observation box, the middle part fixedly connected with transparent panel of observation box.
Furthermore, cultivate the positive one side fixed mounting of box and have first hinge, and cultivate that the box articulates through first hinge has sealing door, cultivate the positive opposite side fixed mounting of box and have quick buckle, cultivate the box and can pass through quick buckle and sealing door fixed connection.
Furthermore, the front surface of the sealing door is fixedly provided with a control panel respectively, the control panel comprises a display panel and a key panel, the display panel is electrically connected with the key panel, and the first motor fan, the second motor fan, the first control valve, the second control valve, the first electric valve, the second electric valve, the ultrasonic atomizer, the electric heating plate, the temperature sensor, the carbon dioxide sensor and the humidity sensor are electrically connected with the control panel respectively.
Furthermore, the first control valve and the second control valve are consistent in specification, the first electric valve and the second electric valve are consistent in specification, the first motor fan and the second motor fan are consistent in specification, and the first vent hole and the second vent hole are consistent in specification.
Furthermore, the working steps are as follows:
s1: placing the microorganism culture dish on the placing plate, further setting the carbon dioxide content range, the temperature range and the humidity range of the microorganism culture growth through the control panel, and detecting the temperature, the carbon dioxide content and the humidity inside the culture box body in real time by the temperature sensor, the carbon dioxide sensor and the humidity sensor;
s2: when the content of carbon dioxide in the culture box body is low, the first motor fan and the first control valve are automatically opened, so that carbon dioxide gas in the compressed gas tank enters the culture box body through the exhaust pipe, and meanwhile, the first motor fan can uniformly disperse the entering carbon dioxide;
s3: when the temperature in the culture box body is lower, the first motor fan and the electric heating plate are automatically started, and then the electric heating plate heats the air in the culture box body through the first motor fan to improve the internal temperature;
s4: when the humidity in the culture box body is low, the first motor fan and the ultrasonic atomizer are automatically started, so that atomized water vapor can be conveyed to the interior of the culture box body through the connecting pipe by the ultrasonic atomizer, and meanwhile, the first motor fan can uniformly disperse the entering water vapor to all parts of the culture box body, so that the humidity in the culture box body is improved;
s5: when cultivateing box carbon dioxide content, temperature or humidity when high on the left, first motorised valve, second motorised valve and second motor fan automatic start, and then the second motor fan can exchange the inside and outside air of incubator through first ventilation hole and second ventilation hole, and then can reduce the inside carbon dioxide content of incubator, temperature or humidity.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the biological culture device and the method thereof, through the arrangement of the compressed gas tank, the ultrasonic atomizer, the first motor fan and the second motor fan, accurate control over the carbon dioxide content and the humidity of the culture environment in the culture box body is achieved, so that a more appropriate microorganism growth environment can be adjusted, and the culture efficiency of microorganisms is improved.
(2) The biological culture device and the method thereof realize that the device is convenient to move through the arrangement of the universal wheels, realize that the temperature in the culture box body is convenient to control through the arrangement of the heating plate, ensure that the culture operation is simpler through the automatic control, and realize that the culture condition in the culture box body is convenient to observe through the arrangement of the observation frame.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a left side view of the present invention;
FIG. 3 is a front view of the present invention with the sealing door removed;
FIG. 4 is a front view of the present invention with the sealing door and viewing frame removed;
FIG. 5 is an enlarged view of portion A of FIG. 1 in accordance with the present invention;
fig. 6 is a bottom view of the placement board according to the present invention.
In the figure: 1. a culture box body; 2. a universal wheel; 3. a first hinge; 4. quickly buckling; 5. a sealing door; 6. a second hinge; 7. an observation frame; 8. a transparent panel; 9. a first vent hole; 10. a second vent hole; 11. a first motor fan; 12. a second motor fan; 13. a first electrically operated valve; 14. a second electrically operated valve; 15. a compressed gas tank; 16. a fixing hoop; 17. an exhaust pipe; 18. a first control valve; 19. an inflation tube; 20. a second control valve; 21. an ultrasonic atomizer; 22. a connecting pipe; 23. an electric hot plate; 24. a temperature sensor; 25. a carbon dioxide sensor; 26. a humidity sensor; 27. a fixing strip; 28. placing the plate; 2801. a porous plate body; 2802. a limiting strip; 2803. and (4) ventilating holes.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that in the description of the present invention, the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, which are only for convenience of description and simplification of description, and do not indicate or imply that the referred device or element must have a specific orientation, be configured in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Further, it will be appreciated that the dimensions of the various elements shown in the figures are not drawn to scale, for ease of description, and that the thickness or width of some layers may be exaggerated relative to other layers, for example.
It should be noted that like reference numerals and letters refer to like items in the following figures, and thus, once an item is defined or illustrated in one figure, it will not need to be further discussed or illustrated in detail in the description of the following figure.
As shown in fig. 1 to 6, the present invention provides a technical solution: a biological culture device and a method thereof comprise a culture box body 1, wherein the top and the bottom of the culture box body 1 are respectively provided with a first vent hole 9 and a second vent hole 10, a first motor fan 11 is fixedly arranged inside the first vent hole 9, a second motor fan 12 is fixedly arranged inside the second vent hole 10, a first electric valve 13 and a second electric valve 14 are respectively and fixedly arranged at the top and the bottom of the culture box body 1, the first electric valve 13 is matched with the first vent hole 9, the second electric valve 14 is matched with the second vent hole 10, one side of the bottom of the culture box body 1 is fixedly connected with a fixed hoop 16, the culture box body 1 is fixedly connected with a compressed gas tank 15 through the fixed hoop 16, one end of the compressed gas tank 15 is fixedly connected with an exhaust pipe 17, the other end of the compressed gas tank 15 is fixedly connected with an inflation pipe 19, the middle part of the exhaust pipe 17 is fixedly provided with a first control valve, middle part fixed mounting of gas tube 19 has second control valve 20, the one end of blast pipe 17 runs through cultivates box 1 and extends to its inside, cultivate the back fixed mounting of box 1 and have ultrasonic nebulizer 21, and the top fixedly connected with connecting pipe 22 of ultrasonic nebulizer 21, connecting pipe 22 extends to the inside of cultivateing box 1, cultivate the equal fixedly connected with fixed strip 27 of inner wall of box 1 both sides, and the top joint of fixed strip 27 has and places board 28, the intermediate position of cultivateing box 1 both sides respectively fixed mounting have carbon dioxide sensor 25 and humidity transducer 26, one side lateral wall fixed mounting at cultivateing box 1 top has temperature sensor 24, the roof fixed mounting of cultivateing box 1 has electric plate 23, and electric plate 23 is located first ventilation hole 9 under.
As shown in fig. 4 and 6, the placing plate 28 includes a porous plate body 2801, and both sides of the bottom of the porous plate body 2801 are fixedly connected with limiting strips 2802, ventilation holes 2803 are formed in the middle of the porous plate body 2801, the ventilation holes 2803 are uniformly distributed, a limiting groove is formed in the top of the fixing strip 27, and the limiting groove is matched with the limiting strips 2802.
As shown in fig. 3, the universal wheels 2 are fixedly connected to four corners of the bottom of the cultivation box body 1, the brake stopping sheet is arranged at the top of each universal wheel 2, the second hinge 6 is fixedly connected to one side of the inside of the cultivation box body 1, the cultivation box body 1 is fixedly connected with the observation frame 7 through the second hinge 6, and the transparent panel 8 is fixedly connected to the middle of the observation frame 7.
As shown in FIG. 1, a first hinge 3 is fixedly installed on one side of the front surface of the cultivation box body 1, the cultivation box body 1 is hinged to a sealing door 5 through the first hinge 3, a quick buckle 4 is fixedly installed on the other side of the front surface of the cultivation box body 1, and the cultivation box body 1 can be fixedly connected with the sealing door 5 through the quick buckle 4.
As shown in fig. 1 and 4, a control panel is fixedly mounted on each of the front surfaces of the sealing door 5, the control panel includes a display panel and a key panel, the display panel is electrically connected to the key panel, and the first motor fan 11, the second motor fan 12, the first control valve 18, the second control valve 20, the first electric valve 13, the second electric valve 14, the ultrasonic atomizer 21, the electric heating plate 23, the temperature sensor 24, the carbon dioxide sensor 25 and the humidity sensor 26 are electrically connected to the control panel.
As shown in fig. 4, the first control valve 18 and the second control valve 20 have the same specification, the first motor-operated valve 13 and the second motor-operated valve 14 have the same specification, the first motor-operated fan 11 and the second motor-operated fan 12 have the same specification, and the first vent hole 9 and the second vent hole 10 have the same specification.
When the device of the invention is needed to be used for culturing microorganisms, firstly, a microorganism culture dish is placed on the placing plate 28, the carbon dioxide content range, the temperature range and the humidity range for the culture growth of the microorganisms are further set through the control panel, and the temperature sensor 24, the carbon dioxide sensor 25 and the humidity sensor 26 detect the temperature, the carbon dioxide content and the humidity in the culture box body 1 in real time; when the content of carbon dioxide in the culture box body 1 is low, the first motor fan 11 and the first control valve 18 are automatically opened, so that carbon dioxide gas in the compressed gas tank 15 enters the culture box body 1 through the exhaust pipe 17, and meanwhile, the first motor fan 11 can uniformly disperse the entering carbon dioxide; when the temperature in the culture box body 1 is lower, the first motor fan 11 and the electric heating plate 23 are automatically started, and then the electric heating plate 23 heats the air in the culture box body 1 through the first motor fan 11 to improve the internal temperature; when the humidity in the culture box body 1 is low, the first motor fan 11 and the ultrasonic atomizer 21 are automatically started, so that the ultrasonic atomizer 21 can convey atomized water vapor to the interior of the culture box body 1 through the connecting pipe 22, and meanwhile, the first motor fan 11 can uniformly disperse the entered water vapor to all parts of the culture box body 1, so that the humidity in the culture box body 1 is improved; when the carbon dioxide content, temperature or humidity of the culture box body 1 is higher, the first electric valve 13, the second electric valve 14 and the second motor fan 12 are automatically started, and then the second motor fan 12 can exchange the air inside and outside the culture box body 1 through the first ventilation hole 9 and the second ventilation hole 10, so that the carbon dioxide content, temperature or humidity inside the culture box body 1 can be reduced.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A biological culture apparatus, includes incubator body (1), its characterized in that: the cultivation box comprises a cultivation box body (1), wherein the top and the bottom of the cultivation box body (1) are respectively provided with a first ventilation hole (9) and a second ventilation hole (10), a first motor fan (11) is fixedly installed inside the first ventilation hole (9), a second motor fan (12) is fixedly installed inside the second ventilation hole (10), the top and the bottom of the cultivation box body (1) are respectively and fixedly provided with a first electric valve (13) and a second electric valve (14), the first electric valve (13) is matched with the first ventilation hole (9), the second electric valve (14) is matched with the second ventilation hole (10), one side of the bottom of the cultivation box body (1) is fixedly connected with a fixed hoop (16), the cultivation box body (1) is fixedly connected with a compressed gas tank (15) through the fixed hoop (16), one end of the compressed gas tank (15) is fixedly connected with an exhaust pipe (17), and the other end fixedly connected with gas tube (19) of compressed gas jar (15), the middle part fixed mounting of blast pipe (17) has first control valve (18), the middle part fixed mounting of gas tube (19) has second control valve (20), the one end of blast pipe (17) runs through cultivates box (1) and extends to its inside, the back fixed mounting of cultivateing box (1) has ultrasonic nebulizer (21), and the top fixedly connected with connecting pipe (22) of ultrasonic nebulizer (21), connecting pipe (22) extend to the inside of cultivateing box (1), the equal fixedly connected with fixed strip (27) of inner wall of cultivateing box (1) both sides, and the top joint of fixed strip (27) has places board (28), the intermediate position of cultivateing box (1) both sides respectively fixed mounting has carbon dioxide sensor (25) and humidity transducer (26), the temperature sensor (24) is fixedly mounted on the side wall of one side of the top of the culture box body (1), the electric heating plate (23) is fixedly mounted on the top wall of the culture box body (1), and the electric heating plate (23) is located under the first ventilation hole (9).
2. A biological growth device according to claim 1, characterised in that: place board (28) including porous plate body (2801), and the equal fixedly connected with of both sides of porous plate body (2801) bottom spacing strip (2802), ventilative hole (2803) have been seted up at the middle part of porous plate body (2801), and ventilative hole (2803) evenly distributed, the spacing groove has been seted up at the top of fixed strip (27), spacing groove and spacing strip (2802) looks adaptation.
3. A biological growth device according to claim 2, characterised in that: cultivate equal fixedly connected with universal wheel (2) in four corners of box (1) bottom, the top of universal wheel (2) is provided with the braking piece, cultivate one side fixedly connected with second hinge (6) of box (1) inside, and cultivate box (1) and pass through second hinge (6) fixedly connected with observation frame (7), the middle part fixedly connected with transparent panel (8) of observation frame (7).
4. A biological growth device according to claim 3, characterised in that: cultivate positive one side fixed mounting of box (1) and have first hinge (3), and cultivate that box (1) articulates through first hinge (3) has sealing door (5), cultivate positive opposite side fixed mounting of box (1) and have quick buckle (4), cultivate box (1) and can pass through quick buckle (4) and sealing door (5) fixed connection.
5. A biological growth device according to claim 4, characterised in that: the front of sealing door (5) is fixed mounting respectively has control panel, control panel includes display panel and key panel, display panel and key panel electric connection, first motor fan (11), second motor fan (12), first control valve (18), second control valve (20), first motorised valve (13), second motorised valve (14), ultrasonic nebulizer (21), electric plate (23), temperature sensor (24), carbon dioxide sensor (25) and humidity transducer (26) respectively with control panel electric connection.
6. A biological growth device according to claim 5, characterised in that: the first control valve (18) and the second control valve (20) are in accordance with each other in specification, the first electric valve (13) and the second electric valve (14) are in accordance with each other in specification, the first motor fan (11) and the second motor fan (12) are in accordance with each other in specification, and the first vent hole (9) and the second vent hole (10) are in accordance with each other in specification.
7. A method of operating a biological growth device according to claim 5, wherein: the working steps are as follows:
s1: the microorganism culture dish is placed on the placing plate (28), the carbon dioxide content range, the temperature range and the humidity range of the microorganism culture growth are further set through the control panel, and the temperature sensor (24), the carbon dioxide sensor (25) and the humidity sensor (26) detect the temperature, the carbon dioxide content and the humidity in the culture box body (1) in real time;
s2: when the content of carbon dioxide in the culture box body (1) is low, the first motor fan (11) and the first control valve (18) are automatically opened, so that carbon dioxide gas in the compressed gas tank (15) enters the culture box body (1) through the exhaust pipe (17), and meanwhile, the first motor fan (11) can uniformly disperse the entering carbon dioxide;
s3: when the temperature in the culture box body (1) is lower, the first motor fan (11) and the electric heating plate (23) are automatically started, and then the electric heating plate (23) heats the air in the culture box body (1) through the first motor fan (11) to improve the internal temperature;
s4: when the humidity in the culture box body (1) is low, the first motor fan (11) and the ultrasonic atomizer (21) are automatically started, the ultrasonic atomizer (21) can convey atomized water vapor to the interior of the culture box body (1) through the connecting pipe (22), and meanwhile, the first motor fan (11) can uniformly disperse the entered water vapor to all parts of the culture box body (1), so that the humidity in the culture box body (1) is improved;
s5: when the carbon dioxide content, the temperature or the humidity of the culture box body (1) are higher, the first electric valve (13), the second electric valve (14) and the second motor fan (12) are automatically started, and then the second motor fan (12) can exchange the air inside and outside the culture box body (1) through the first ventilation hole (9) and the second ventilation hole (10), so that the carbon dioxide content, the temperature or the humidity inside the culture box body (1) can be reduced.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011165232.4A CN112226357A (en) | 2020-10-27 | 2020-10-27 | Biological culture device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011165232.4A CN112226357A (en) | 2020-10-27 | 2020-10-27 | Biological culture device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112226357A true CN112226357A (en) | 2021-01-15 |
Family
ID=74110231
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011165232.4A Pending CN112226357A (en) | 2020-10-27 | 2020-10-27 | Biological culture device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112226357A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146575A (en) * | 2013-01-21 | 2013-06-12 | 重庆医科大学附属永川医院 | Small-scale carbon dioxide cell cultivating cabinet with fluid interface and electrical interface |
| CN208844101U (en) * | 2018-07-11 | 2019-05-10 | 吉林济惠生物科技有限公司 | A kind of carbon dioxide incubator |
| CN110468043A (en) * | 2019-08-15 | 2019-11-19 | 柳州市妇幼保健院 | A kind of Embryo Culture case |
| WO2020021874A1 (en) * | 2018-07-23 | 2020-01-30 | 株式会社エアレックス | Method for controlling positive pressure of incubator, and culturing system using same |
| CN110833006A (en) * | 2019-10-29 | 2020-02-25 | 闵天威 | Device for hemp growth micro intelligent environment and operation method |
-
2020
- 2020-10-27 CN CN202011165232.4A patent/CN112226357A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146575A (en) * | 2013-01-21 | 2013-06-12 | 重庆医科大学附属永川医院 | Small-scale carbon dioxide cell cultivating cabinet with fluid interface and electrical interface |
| CN208844101U (en) * | 2018-07-11 | 2019-05-10 | 吉林济惠生物科技有限公司 | A kind of carbon dioxide incubator |
| WO2020021874A1 (en) * | 2018-07-23 | 2020-01-30 | 株式会社エアレックス | Method for controlling positive pressure of incubator, and culturing system using same |
| CN110468043A (en) * | 2019-08-15 | 2019-11-19 | 柳州市妇幼保健院 | A kind of Embryo Culture case |
| CN110833006A (en) * | 2019-10-29 | 2020-02-25 | 闵天威 | Device for hemp growth micro intelligent environment and operation method |
Non-Patent Citations (2)
| Title |
|---|
| 杨军等: "一种便携式细胞培养箱的研制", 《传感器与微***》 * |
| 美国压缩气体协会, 上海科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113088451A (en) | Biological incubator for experiments | |
| CN112266869A (en) | Laboratory is with little biological culture device | |
| CN110951589A (en) | Microbial detection clean culture device and use method thereof | |
| CN214193279U (en) | High-efficient microbial strain cultivates equipment | |
| CN112226357A (en) | Biological culture device and method | |
| CN215162696U (en) | Medical science inspection microbiological culture apparatus | |
| CN210656946U (en) | Aseptic incubator is used in biological detection | |
| CN109022265A (en) | A kind of microbiological incubator | |
| CN205387577U (en) | Novel carbon dioxide cell incubator | |
| CN113797368A (en) | Two-in-one efficient sterilization incubator | |
| CN213662848U (en) | Edible fungus culture device | |
| CN208175599U (en) | A kind of flat mushroom strain fermenting tank | |
| CN213708382U (en) | A safe biological culture apparatus for microbial cultivation | |
| CN208104425U (en) | A kind of forced convertion incubator | |
| CN209052706U (en) | The cultivating equipment of microorganism detection | |
| CN221296914U (en) | Marine microorganism microbial inoculum incubator | |
| CN216712096U (en) | Can automated inspection and adjust bacterial culture case of humiture | |
| CN218596368U (en) | High-temperature sterilization three-gas incubator | |
| CN215209460U (en) | Culture box is used in processing of toad venom injection | |
| CN217838973U (en) | Culture apparatus is used in microbial inoculant production convenient to temperature regulation | |
| CN213113340U (en) | Cell culture device for biotechnology | |
| CN206570337U (en) | A kind of air microbe biochemical cultivation case | |
| CN221429741U (en) | Edible fungus strain cultivation bottle | |
| CN217064834U (en) | Artificial phellinus igniarius spore liquid submerged culture device based on high altitude area | |
| CN218478763U (en) | Microbial cultivation integration equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210115 |