CN112210496A - Embryonic stem cell induced differentiation instrument - Google Patents
Embryonic stem cell induced differentiation instrument Download PDFInfo
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- CN112210496A CN112210496A CN202011115221.5A CN202011115221A CN112210496A CN 112210496 A CN112210496 A CN 112210496A CN 202011115221 A CN202011115221 A CN 202011115221A CN 112210496 A CN112210496 A CN 112210496A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
Abstract
The invention discloses an embryonic stem cell induced differentiation instrument, which comprises a machine body, wherein a liquid cavity with an upward opening is arranged on the top wall of the machine body, a hose is communicated with the bottom wall of the liquid cavity, a push rod cavity is arranged in the bottom wall of the liquid cavity, and limiting cavities are symmetrically arranged in the left wall and the right wall of the push rod cavity.
Description
Technical Field
The invention relates to the technical field of biology, in particular to an embryonic stem cell induced differentiation instrument.
Background
Stem cell induced differentiation is induced stem cell directed differentiation, make it become ripe functional cell, be the key link of stem cell research at present, in induced differentiation process, need add corresponding induced liquid, however induced liquid is mostly artifical at present and adds, face in a large amount of contrast experiment processes, artifical the interpolation leads to certain time difference, but reduce the time difference to a certain extent through mechanical addition, reduce the influence of time to the contrast experiment, examine simultaneously in the embryonic stem cell induced differentiation process, need take out stem cell from the culture environment, there is the possibility of polluting the culture medium, therefore it is necessary to design an embryonic stem cell induced differentiation instrument.
Disclosure of Invention
The invention aims to provide an embryonic stem cell induced differentiation instrument, which solves the problems of automatic addition of an induction liquid, centrifugation and cell activity detection of embryonic stem cells.
The invention is realized by the following technical scheme.
The invention relates to an embryonic stem cell induced differentiation instrument, which comprises a machine body, wherein a liquid cavity with an upward opening is arranged on the top wall of the machine body, a hose is communicated with the bottom wall of the liquid cavity, a push rod cavity is arranged in the bottom wall of the liquid cavity, limiting cavities are symmetrically arranged in the left wall and the right wall of the push rod cavity, a door control device for opening and closing a door only on one side by using a push rod and an incomplete gear is arranged in the limiting cavities, an incomplete gear cavity is arranged in the rear wall of the machine body, a door motor with a front output shaft is fixedly arranged in the rear wall of the incomplete gear cavity, a door motor shaft extending forwards and backwards is fixedly arranged at the tail end of an output shaft of the door motor, one end of the door motor shaft, far away from the door motor, extends into the incomplete gear cavity, an incomplete gear is fixedly arranged at the tail end of the door, a main gear is fixedly arranged at one end of the auxiliary shaft extending into the incomplete gear cavity, one end of the auxiliary shaft far away from the main gear extends forwards into the belt wheel cavity, a main belt wheel is fixedly arranged at the tail end of the auxiliary shaft, door shafts extending forwards and backwards are symmetrically arranged on the front wall of the belt wheel cavity in a bilateral mode, one end of each door shaft extending into the belt wheel cavity is fixedly provided with an auxiliary belt wheel, the bottom wall of the limiting cavity is communicated with a door cavity penetrating through the channel, a door gear cavity is communicated and arranged on the wall of the door cavity close to the door shaft, one end of each door shaft far away from the auxiliary belt wheel extends into the door gear cavity, a door gear is fixedly arranged at the tail end of each door shaft, the auxiliary belt wheels are in transmission connection with the nearest main belt wheel through a transmission belt, an outer moving cavity is arranged in the bottom wall of the push rod cavity, a culture chamber is communicated and arranged on, the cultivation room is bilaterally symmetrical and is communicated with a channel communicated with the outside of the machine body, one end of the rotary plate shaft far away from the rotary plate motor upwards extends into the cultivation room, the tail end of the rotary plate shaft is fixedly provided with a rotary plate, the rotary plate top wall is provided with a carrier cavity with an upward opening around a circle center array, a carrier is placed in the carrier cavity, the bottom wall of the carrier cavity is communicated with a withdrawing cavity, a cultivation device for limiting and pushing out the carrier through a push rod and a gear rack is arranged in the withdrawing cavity, a spline spindle for realizing extension is arranged at the circle center of the rotary plate top wall, one end of the spline spindle far away from the rotary plate is fixedly provided with a disc, a movable rod extending up and down is rotationally arranged in the disc top wall, one end of the movable rod far away from the disc extends into the top, the top wall of the culture chamber is fixedly provided with a cover plate compression spring, one end of the cover plate compression spring, far away from the top wall of the culture chamber, is abutted against the disc, the right wall of the outer moving cavity is fixedly provided with a moving electromagnet, the right wall of the outer moving cavity is fixedly provided with a moving compression spring, one end of the moving compression spring, far away from the right wall of the outer moving cavity, is fixedly provided with an injection shell extending leftwards and rightwards, an inner moving cavity is arranged in the injection shell, an injection moving shell sliding forwards and backwards is arranged in the inner moving cavity, a cam cavity is arranged in the injection moving shell, the bottom wall of the cam cavity is fixedly provided with an injection compression spring, one end of the injection compression spring, far away from the bottom wall of the cam cavity is fixedly provided with an injection pressure plate, the bottom wall of the injection pressure plate is, the injection device is characterized in that a grooved wheel cavity is formed in the rear wall of the inner moving cavity, a dosing device which realizes that the injector is in an inverted 'mountain' type motion track through the cooperation of a cam and a lead screw nut with a grooved wheel is arranged in the grooved wheel cavity, a reversing cavity is formed in the rear wall of the outer moving cavity, and a power device which provides power for the dosing device is arranged in the reversing cavity.
Preferably, be provided with the door that realizes extending in the door chamber, the door is close to fixed being provided with on the wall of door gear with door gear mesh's door rack, the door is kept away from the fixed door spring piece that is provided with in top of the wall of door rack, the fixed door compression spring that is provided with of door spring piece diapire, door compression spring keeps away from the one end of door spring piece is fixed on the restriction chamber diapire, the push rod that extends about being provided with of push rod intracavity, stretch into both sides respectively about the push rod restriction chamber and butt the door roof, the fixed push rod plate that is provided with of push rod roof, the fixed push rod compression spring that is provided with of push rod plate left wall, push rod compression spring keeps away from the one end of push rod plate is fixed on the push rod chamber left wall.
Preferably, a stop block compression spring is fixedly arranged on the bottom wall of the withdrawing cavity far away from the center of the rotary table, a stop block is fixedly arranged at one end of the stop block compression spring far away from the bottom wall of the withdrawing cavity, a limit push rod which horizontally extends towards the center of the rotary table and is abutted against the stop block is arranged on the bottom wall of the withdrawing cavity, a lower rack is fixedly arranged on the top wall of the limit push rod, an L rod is fixedly arranged at one end of the limit push rod close to the center of the rotary table, a reset compression spring which horizontally extends is fixedly arranged at one end of the L rod close to the center of the rotary table, a gear shaft which vertically extends in the direction of the limit push rod is arranged above the lower rack, a gear which is meshed with the lower rack is fixedly arranged on the outer surface of the gear shaft, an exit rod extending perpendicular to the gear shaft direction is fixedly arranged at the upper end of the upper rack, a push block abutting against the carrier is fixedly arranged at one end of the exit rod away from the center of the rotary table, an exit compression spring is fixedly arranged on the wall of the push block close to the center of the rotary table, one end of the exit compression spring away from the push block is fixed on the wall of the exit cavity close to the center of the rotary table, the bottom wall of one end of the exit rod away from the push block is fixedly provided with an exit electromagnet abutting against the wall of the L rod away from the center of the rotary table, the wall of the exit cavity close to the center of the rotary table is fixedly provided with an exit electromagnet, a slow speed cavity is arranged in the wall of the exit cavity close to the center of the rotary table, a torsion spring shaft extending parallel to the exit rod direction is arranged in the slow speed cavity, the outer surface of the wire wheel is wound and fixedly provided with a pull wire, one end of the pull wire, which is far away from the wire wheel, is fixed on the push block, the retarding cavity is close to a spring cavity which is arranged in the wall of the circle center of the turntable, the spring cavity is far away from a compression spring which is fixedly arranged on the wall of the circle center of the turntable, one end of the compression spring, which is close to the circle center of the turntable, is fixedly provided with a spring plate, and the top wall of the spring plate is fixedly.
Preferably, the sheave cavity is provided with axles extending forward and backward in an up-down symmetrical manner, the part of the outer surface of the axle close to the rear wall of the sheave cavity is fixedly provided with an injection gear, the part of the outer surface of the axle close to the front wall of the sheave cavity is fixedly provided with sheaves, four groups of sheave grooves are uniformly arranged on the circumferential wall of the sheave at ninety degrees, the rear wall of the sheave cavity is provided with an injection power shaft extending forward and backward, one end of the injection power shaft extending into the sheave cavity is fixedly provided with a main drive plate, the wall of the main drive plate close to the sheave is fixedly provided with two groups of cylindrical pins at ninety degrees, the cylindrical pins enter the sheave grooves along with the rotation of the main drive plate to drive the sheave to rotate, the lower side of the front wall of the sheave cavity is provided with an outer shaft extending forward and backward, the spline shaft is provided with a spline shaft extending forward and backward, one end of the spline shaft far away from the outer shaft, the montant is kept away from the fixed one end of integral key shaft be provided with the downside the injection pinion that pours into gear engagement, the antetheca upside of groove wheel chamber is provided with the fore-and-aft movement axle that extends from beginning to end, the fore-and-aft movement axle stretches into the one end in groove wheel chamber is fixed be provided with the upside the back-and-forth movement gear that pours into gear engagement, the fore-and-aft movement axle is kept away from the one end of back-and-forth movement gear is fixed to be provided with the fore-and-aft extension and runs through remove the chamber with the lead screw of injecting into the removal shell, the lead screw with it removes shell threaded connection to inject into, the power axle is kept away.
Preferably, an injection motor with a forward output shaft is fixedly arranged in the rear wall of the reversing cavity, an injection motor shaft extending forwards and backwards is fixedly arranged at the tail end of the output shaft of the injection motor, a reversing main gear is fixedly arranged on the outer surface of the portion, close to the rear wall of the reversing cavity, of the injection motor shaft, a backward gear meshed with the injection power gear is fixedly arranged on the outer surface of the portion, close to the front wall of the reversing cavity, of the injection motor shaft, a reversing shaft extending forwards and backwards is arranged on the right side of the reversing cavity, a reversing auxiliary gear meshed with the reversing main gear is fixedly arranged on the outer surface of the portion, close to the rear wall of the reversing cavity, of the reversing shaft, and a forward.
The invention has the beneficial effects that: the grooved pulley drives the cam to match with the spring to realize the up-and-down movement of the injector so as to control the injection of the inducing liquid, the grooved pulley drives the screw rod to realize the movement of the injector, simultaneously, the two groups of grooved pulleys alternately rotate to realize the inverted mountain-shaped movement of the injector, the injector has enough time to inject the inducing liquid after moving downwards by matching with the two cylindrical pins, simultaneously, the two side doors only allow one side to be opened by the push rod, the pollution of a culture medium is reduced, and simultaneously, the disc is utilized to push the sliding cover with the slope to cover the carrier, so that the inducing liquid is prevented from spilling out and the culture medium.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of the present invention;
FIG. 2 is a schematic structural diagram at A-A in FIG. 1 according to an embodiment of the present invention;
FIG. 3 is a schematic structural diagram at B-B in FIG. 1 according to an embodiment of the present invention;
FIG. 4 is a schematic structural diagram at C-C in FIG. 3 according to an embodiment of the present invention;
FIG. 5 is a schematic structural diagram at D-D in FIG. 4 according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of the structure at E-E in FIG. 3 according to an embodiment of the present invention;
FIG. 7 is a schematic diagram of the structure at F-F in FIG. 3 according to an embodiment of the present invention;
FIG. 8 is an enlarged schematic view of the embodiment of the present invention at G in FIG. 1;
FIG. 9 is an enlarged view of the embodiment of the present invention shown in FIG. 3 at H.
Detailed Description
The invention will now be described in detail with reference to fig. 1-9, wherein for ease of description the orientations described below are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
The embryonic stem cell induced differentiation instrument described in conjunction with the attached figures 1-9 comprises a machine body 1, wherein a liquid cavity 38 with an upward opening is arranged on the top wall of the machine body 1, a hose 39 is arranged on the bottom wall of the liquid cavity 38 in a communicating manner, a push rod cavity 35 is arranged in the bottom wall of the liquid cavity 38, a limiting cavity 2 is symmetrically arranged in the left wall and the right wall of the push rod cavity 35, a door control device 901 for realizing only one-side door opening and closing by using a push rod and an incomplete gear is arranged in the limiting cavity 2, an incomplete gear cavity 17 is arranged in the rear wall of the machine body 1, a door motor 57 with a forward output shaft is fixedly arranged in the rear wall of the incomplete gear cavity 17, a door motor shaft 58 extending forwards and backwards is fixedly arranged at the tail end of the output shaft of the door motor 57, one end of the door motor shaft 58 far away, the front wall of the incomplete gear cavity 17 is provided with a belt wheel cavity 50, the front wall of the incomplete gear cavity 17 is bilaterally symmetrically provided with a counter shaft 54 extending front and back, the counter shaft 54 extends into one end of the incomplete gear cavity 17 and is fixedly provided with a main gear 55, one end of the counter shaft 54 far away from the main gear 55 extends forward into the belt wheel cavity 50 and the tail end of the belt wheel 53, the front wall of the belt wheel cavity 50 is bilaterally symmetrically provided with a front door shaft 49 extending front and back, one end of the door shaft 49 extends into the belt wheel cavity 50 and is fixedly provided with a counter pulley 51, the bottom wall of the limiting cavity 2 is communicated with a door cavity 7 penetrating through a channel 9, the door cavity 7 is close to the wall of the door shaft 49 and is communicated with a door gear cavity 48, one end of the door shaft 49 far away from the counter pulley 51 extends into the door gear cavity 48 and the tail end of the door gear 47, and the counter pulley 51 is in transmission connection with the main, the utility model discloses a culture device, including push rod chamber 35 diapire, removal chamber 46, carousel shaft 24, carousel motor 23, culture chamber 11, carrier chamber 16, carrier chamber 28, carousel shaft 28, outer removal chamber 46 diapire intercommunication is provided with culture chamber 11, culture chamber 11 roof right side internal fixation is provided with activity detector 10, culture chamber 11 bilateral symmetry and intercommunication are provided with intercommunication the outer passageway 9 of fuselage 1, the one end that carousel shaft 24 kept away from carousel motor 23 upwards stretches into culture chamber 11 and the end is fixed and is provided with carousel 15, carousel 15 roof is provided with the ascending carrier chamber 16 of opening around the centre of a circle array, carrier 20 has been placed in the carrier chamber 16 diapire intercommunication, be provided with and withdraw from chamber 28, it realizes through push rod cooperation rack and pinion cultivation device 902 spacing and release to carrier 20 to withdraw from to be provided with in the chamber 28, carousel 15 roof centre of a circle department spline is provided, a moving rod 32 extending up and down is rotationally arranged in the top wall of the disc 82, one end of the moving rod 32 far away from the disc 82 extends into the top wall of the culture chamber 11, a cover plate electromagnet 33 is fixedly arranged above the moving rod 32, a cover plate compression spring 31 is fixedly arranged on the top wall of the culture chamber 11, one end of the cover plate compression spring 31 far away from the top wall of the culture chamber 11 is abutted against the disc 82, a moving electromagnet 67 is fixedly arranged on the right wall of the outer moving cavity 46, a moving compression spring 68 is fixedly arranged on the right wall of the outer moving cavity 46, an injection shell 44 extending left and right is fixedly arranged at one end of the moving compression spring 68 far away from the right wall of the outer moving cavity 46, an inner moving cavity 69 is arranged in the injection shell 44, an injection moving shell 45 sliding back and forth is arranged in the inner moving cavity 69, and a cam cavity 43, the fixed injection compression spring 41 that is provided with of cam chamber 43 diapire, injection compression spring 41 keeps away from the fixed injection clamp plate 42 that is provided with of one end of cam chamber 43 diapire, the fixed syringe 40 that is provided with of injection clamp plate 42 diapire, syringe 40 downwardly extending cultivates in the room 11, syringe 40 is connected hose 39, it is provided with grooved wheel chamber 73 to move the chamber 69 back wall in, it realizes through cam and screw nut cooperation sheave to be provided with in the grooved wheel chamber 73 the syringe 40 is the charge device 903 of "mountain" type motion orbit, be provided with switching-over chamber 66 in the outer removal chamber 46 back wall, be provided with in the switching-over chamber 66 into power device 904 that charge device 903 provides power.
Advantageously, a door 6 extending is arranged in the door cavity 7, a door rack 8 engaged with the door gear 47 is fixedly arranged on the wall of the door 6 close to the door gear 47, the top end of the wall of the door 6 far away from the door rack 8 is fixedly provided with a door spring block 4, the bottom wall of the door spring block 4 is fixedly provided with a door compression spring 5, one end of the door compression spring 5 far away from the door spring block 4 is fixed on the bottom wall of the limiting cavity 2, a push rod 3 extending left and right is arranged in the push rod cavity 35, the push rod 3 extends into the limiting cavities 2 at two sides left and right respectively and is abutted against the top wall of the door 6, a push rod plate 36 is fixedly arranged on the top wall of the push rod 3, a push rod compression spring 37 is fixedly arranged on the left wall of the push rod plate 36, one end of the push rod compression spring 37 far away from the push rod plate 36 is fixed on the left wall of the push rod cavity 35.
Beneficially, a stopper compression spring 22 is fixedly arranged on the bottom wall of the withdrawing cavity 28 away from the center of the circle of the rotating disc 15, a stopper 21 is fixedly arranged on one end of the stopper compression spring 22 away from the bottom wall of the withdrawing cavity 28, a limiting push rod 29 which extends horizontally towards the center of the circle of the rotating disc 15 and abuts against the stopper 21 is arranged on the bottom wall of the withdrawing cavity 28, a lower rack 25 is fixedly arranged on the top wall of the limiting push rod 29, an L-shaped rod 26 is fixedly arranged on one end of the limiting push rod 29 close to the center of the circle of the rotating disc 15, a return compression spring 98 which extends leftwards and rightwards is fixedly arranged on one end of the L-shaped rod 26 close to the center of the circle of the rotating disc 15, a gear shaft 96 which extends perpendicular to the direction of the limiting push rod 29 is arranged above the lower, an upper rack 95 extending perpendicular to the direction of the gear shaft 96 is arranged above the gear 18 in a meshed manner, an exit rod 97 extending perpendicular to the direction of the gear shaft 96 is fixedly arranged at the upper end of the upper rack 95, a push block 19 abutting against the carrier 20 is fixedly arranged at one end, far away from the center of the rotary table 15, of the exit rod 97, an exit compression spring 93 is fixedly arranged on the wall, close to the center of the rotary table 15, of the push block 19, one end, far away from the push block 19, of the exit compression spring 93 is fixed on the wall, close to the center of the rotary table 15, of the exit cavity 28, an exit electromagnet 91 is fixedly arranged in the wall, close to the center of the rotary table 15, of the exit cavity 28, a retarding cavity 88 is arranged in the wall, close to the center of the rotary table 15, of the exit cavity 28, be provided with the parallel in the slow chamber 88 the torsion spring axle 89 that the direction of withdrawing rod 97 extends, torsion spring axle 89 external surface fixation is provided with line wheel 94, the external surface of line wheel 94 coils and fixedly is provided with the 92 of acting as go-between, the 92 of acting as go-between is kept away from the one end of line wheel 94 is fixed on the ejector pad 19, slow chamber 88 is close to be provided with spring chamber 86 in the wall in the carousel 15 centre of a circle, spring chamber 86 is kept away from the fixed compression spring 87 that is provided with on the wall in the carousel 15 centre of a circle, compression spring 87 is close to the fixed spring plate 84 that is provided with in one end in the carousel 15 centre of a circle, the fixed apron 85 that is.
Beneficially, the sheave cavity 73 is provided with an axle 75 extending forward and backward symmetrically, a part of the outer surface of the axle 75 near the rear wall of the sheave cavity 73 is fixedly provided with an injection gear 72, a part of the outer surface of the axle 75 near the front wall of the sheave cavity 73 is fixedly provided with a sheave 74, four sets of sheave grooves 81 are uniformly arranged on the circumferential wall of the sheave 74 at ninety degrees, the rear wall of the sheave cavity 73 is provided with an injection power shaft 71 extending forward and backward, one end of the injection power shaft 71 extending into the sheave cavity 73 is fixedly provided with a main drive plate 76, two sets of cylindrical pins 80 are fixedly arranged on the wall of the main drive plate 76 near the sheave 74 at ninety degrees, the cylindrical pins 80 enter the sheave grooves 81 with the rotation of the main drive plate 76 to drive the sheave 74 to rotate, a front wall lower side of the sheave cavity 73 is provided with a front and back extending outer shaft 12, and a front and back extending spline shaft 13 is arranged inside, one end of the spline shaft 13 far away from the outer shaft 12 extends into the cam cavity 43 and the tail end is fixedly provided with a cam 14 abutting against the injection compression spring 41, one end of the vertical rod 27 far away from the spline shaft 13 is fixedly provided with an injection pinion 30 meshed with the injection gear 72 at the lower side, a front and rear moving shaft 78 extending forwards and backwards is arranged on the upper side of the front wall of the sheave cavity 73, a front and rear moving gear 77 meshed with the injection gear 72 on the upper side is fixedly arranged at one end of the front and rear moving shaft 78 extending into the sheave cavity 73, a screw 79 extending forward and backward and penetrating the inner moving chamber 69 and the injection moving housing 45 is fixedly arranged at one end of the forward and backward moving shaft 78 away from the forward and backward moving gear 77, the lead screw 79 is in threaded connection with the injection moving shell 45, one end of the injection power shaft 71, which is far away from the main drive plate 76, extends into the reversing cavity 66, and the tail end of the injection power shaft is fixedly provided with an injection power gear 70.
Advantageously, an injection motor 59 with a forward output shaft is fixedly arranged in the rear wall of the reversing cavity 66, a forward injection motor shaft 60 extending forwards and backwards is fixedly arranged at the tail end of the output shaft of the injection motor 59, a reversing main gear 61 is fixedly arranged on the outer surface of the injection motor shaft 60 close to the rear wall part of the reversing cavity 66, a backward moving gear 62 engaged with the injection power gear 70 is fixedly arranged on the outer surface of the injection motor shaft 60 close to the front wall part of the reversing cavity 66, a reversing shaft 64 extending forwards and backwards is arranged on the right side of the reversing cavity 66, a reversing auxiliary gear 63 engaged with the reversing main gear 61 is fixedly arranged on the outer surface of the reversing shaft 64 close to the rear wall part of the reversing cavity 66, and a forward moving gear 65 is fixedly arranged on the outer surface of the.
In the initial state, the injection moving housing 45 is located at the front wall of the inner moving chamber 69, the moving electromagnet 67 is not electromagnetic, the moving compression spring 68 is not loaded, so that the injection housing 44 is located at the left side of the outer moving chamber 46, the door 6 blocks the passage 9, the door compression spring 5 and the push rod compression spring 37 are not loaded, the push rod plate 36 and the push rod 3 are located at the middle of the push rod chamber 35, the cover electromagnet 33 electromagnetically adsorbs the moving rod 32, the disk 82 moves upwards, the cover compression spring 31 is compressed, the compression spring 87 is unloaded, so that the cover 85 is far away from the carrier chamber 16, the withdrawing electromagnet 91 is not electromagnetic, the carrier 20 is not placed in the carrier chamber 16, the withdrawing compression spring 93 is unloaded, the push block 19 extends into the carrier chamber 16, and the upper rack 95 is located at the side of the gear 18 far away from the center of the rotary table 15, the limit push rod 29 is located at one end of the withdrawing cavity 28 close to the circle center of the rotary table 15, the stop 21 is located at the bottom wall of the withdrawing cavity 28, the stop compression spring 22 has no load, the wire wheel 94 releases the pull wire 92, the torsion spring shaft 89 is twisted, the cylindrical pin 80 is located on the right side of the main drive plate 76, the limit push rod 29 has no electromagnetism, the stop 21 has no load, so that the withdrawing cavity 28 and the rotary table shaft 24 are connected through the L-shaped rod 26 in a splined mode, the rotary table 15 is separated from the incomplete gear cavity 17, and the liquid cavity 38 is filled with corresponding inducing liquid.
When the instrument starts to work, the door motor 57 rotates counterclockwise to drive the door motor shaft 58 to rotate counterclockwise, the door motor shaft 58 rotates counterclockwise to drive the incomplete gear 56 to rotate counterclockwise, the incomplete gear 56 rotates counterclockwise to drive the left main gear 55 to rotate counterclockwise, the left main gear 55 rotates counterclockwise to drive the left auxiliary shaft 54 to rotate counterclockwise, the auxiliary shaft 54 rotates counterclockwise to drive the left main pulley 53 to rotate counterclockwise, the left main pulley 53 rotates counterclockwise to drive the left auxiliary pulley 51 to rotate counterclockwise through the left transmission belt 52, the left auxiliary pulley 51 rotates counterclockwise to drive the left door shaft 49 to rotate counterclockwise, the left door shaft 49 rotates counterclockwise to drive the left door gear 47 to rotate counterclockwise, the left door gear 47 rotates counterclockwise to drive the left door rack 8 to move upward, the left side door rack 8 drives the left side door 6 moves upwards, the left side door 6 drives the left side door spring block 4 moves upwards, the left side door spring block 4 drives the left side door compression spring 5 is pulled, the left side door 6 pushes the push rod 3 to move to the right, the push rod 3 drives the push rod plate 36 to move to the right, the push rod compression spring 37 is pulled, and the left side door opening is completed.
Then, the carrier 20 is manually pushed into the carrier cavity 16 from the left side through the channel 9, the carrier 20 pushes the push block 19 to be close to the center of the rotary table 15, the push block 19 drives the withdrawing rod 97 to be close to the center of the rotary table 15, the withdrawing rod 97 drives the upper rack 95 and the vertical rod 27 to be close to the center of the rotary table 15, the withdrawing compression spring 93 is pressed, the torsion spring shaft 89 rotates to drive the wire wheel 94 to rotate and wind the pull wire 92, the upper rack 95 drives the gear 18 to rotate, the gear 18 drives the lower rack 25 to be away from the center of the rotary table 15, the lower rack 25 drives the limiting push rod 29 to be away from the center of the rotary table 15, the limiting push rod 29 drives the L rod 26 to move right to abut against the vertical rod 27, at this time, the upper rack 95 is separated from the gear 18, the limiting push rod 29 pushes the stopper 21 to move up to limit the carrier 20 to leave the carrier cavity 16, the stopper compression spring 22 is pulled, the turntable motor 23 rotates to drive the turntable shaft 24 to rotate, the turntable shaft 24 rotates to drive the turntable 15 to rotate, and the turntable 15 rotates to drive the carrier cavities 16 without the carriers 20 to face the channel 9 on the left side until the carriers 20 are placed in all the carrier cavities 16, so that the placement of the carriers 20 is completed.
The clockwise rotation of the door motor 57 drives the clockwise rotation of the door motor shaft 58, the clockwise rotation of the door motor shaft 58 drives the clockwise rotation of the incomplete gear 56, the clockwise rotation of the incomplete gear 56 drives the clockwise rotation of the left main gear 55, the clockwise rotation of the left main gear 55 drives the clockwise rotation of the left auxiliary shaft 54, the clockwise rotation of the auxiliary shaft 54 drives the clockwise rotation of the left main pulley 53, the clockwise rotation of the main pulley 53 drives the clockwise rotation of the left auxiliary pulley 51 through the left side transmission belt 52, the clockwise rotation of the left auxiliary pulley 51 drives the clockwise rotation of the left door shaft 49, the clockwise rotation of the left door shaft 49 drives the clockwise rotation of the left door gear 47, the clockwise rotation of the left door gear 47 drives the left door rack 8 to move downwards, the left door rack 8 drives the left door 6 to move downwards, the left side door 6 drives the left side door spring block 4 moves downwards, the left side door compression spring 5 recovers no load, the left side door 6 relieves the limit of the left movement of the push rod 3, the push rod compression spring 37 rebounds to drive the push rod plate 36 to move left, the push rod plate 36 drives the push rod 3 to move left, and the left side door closing is completed.
Then the injection motor 59 rotates to drive the injection motor shaft 60 to rotate, the injection motor shaft 60 rotates to drive the backward gear 62 to rotate, the backward gear 62 rotates to drive the injection power gear 70 to rotate, the injection power gear 70 rotates to drive the injection power shaft 71 to rotate, the injection power shaft 71 rotates to drive the main dial 76 to rotate, the main dial 76 rotates to drive the first cylindrical pin 80 from the bottom to the top on the right side to enter the wheel groove 81 of the lower side of the sheave 74 to drive the lower side of the sheave 74 to rotate for a quarter of a circle, the lower side of the sheave 74 drives the lower side of the wheel shaft 75 to rotate, the lower side of the wheel shaft 75 drives the lower side of the injection gear 72 to rotate, the lower side of the injection gear 72 drives the injection pinion 30 to rotate for a half of a circle, and the injection pinion 30 drives the outer shaft 12 to rotate, the outer shaft 12 rotates to drive the spline shaft 13 to rotate, the spline shaft 13 rotates to drive the cam 14 to rotate, the cam 14 rotates to drive the injection pressing plate 42 to move downwards, the injection pressing plate 42 drives the injector 40 to move downwards, the injection compression spring 41 is pressed, the liquid cavity 38 injects an inducing liquid into one cell of the carrier 20 through the hose 39 and the injector 40, then the second cylindrical pin 80 enters the wheel groove 81 of the lower side sheave 74 to drive the lower side sheave 74 to rotate for a quarter of a circle, the lower side sheave 74 drives the lower side wheel shaft 75 to rotate, the lower side wheel shaft 75 drives the lower side injection gear 72 to rotate, the lower side injection gear 72 drives the injection auxiliary gear 30 to rotate for a half of a circle, the injection auxiliary gear 30 drives the outer shaft 12 to rotate, and the outer shaft 12 rotates to drive the spline shaft 13 to rotate, the spline shaft 13 rotates to drive the cam 14 to rotate, after the farthest off-axis point of the cam 14 leaves the injection pressure plate 42, the injection compression spring 41 rebounds to rotate to drive the injection pressure plate 42 to move upwards, the injection pressure plate 42 drives the injector 40 to move upwards, the injection of the inducing liquid into the carrier 20 is stopped, a time difference exists between the rotation of the lower-side sheave 74 driven by the first cylindrical pin 80 and the rotation of the second cylindrical pin 80, the time difference is enough to inject the inducing liquid into the carrier 20, then the first cylindrical pin 80 enters the sheave groove 81 in the upper-side sheave 74 to drive the upper-side sheave 74 to rotate by one fourth, the upper-side sheave 74 drives the upper-side wheel shaft 75 to rotate, the upper-side wheel shaft 75 rotates to drive the upper-side injection gear 72 to rotate, and the upper-side injection gear 72 rotates to drive the back-, the front and rear moving gear 77 rotates to drive the front and rear moving shaft 78 to rotate, the front and rear moving shaft 78 drives the lead screw 79 to rotate, the screw 79 rotates to drive the injection moving shell 45 to move backwards, the second cylindrical pin 80 enters the wheel groove 81 in the upper-side wheel groove 74 to drive the upper-side wheel groove 74 to rotate by a quarter, the upper-side wheel groove 74 drives the upper-side wheel shaft 75 to rotate, the upper-side wheel shaft 75 rotates to drive the upper-side injection gear 72 to rotate, and the upper-side injection gear 72 rotates to drive the front-back moving gear 77 to rotate, the front and rear moving gear 77 rotates to drive the front and rear moving shaft 78 to rotate, the front and rear moving shaft 78 drives the lead screw 79 to rotate, the screw 79 rotates to drive the injection moving shell 45 to move backwards, so that the injector 40 reaches the second grid of the carrier 20, and the injection of the inducing liquid into the first grid of the carrier 20 is completed after the operations are repeated three times.
Starting to inject inducing liquid into the second row of lattices of the carrier 20, the movable electromagnet 67 generates electromagnetic attraction to move the injection housing 44 to the right, the movable compression spring 68 is pressed, the injection housing 44 drives the injection power shaft 71 to move to the right, the injection power shaft 71 drives the injection power gear 70 to move to the right to be engaged with the advancing gear 65, the injection motor 59 rotates to drive the injection motor shaft 60 to rotate, the injection motor shaft 60 rotates to drive the reversing main gear 61 to rotate, the reversing main gear 61 rotates to drive the reversing auxiliary gear 63 to rotate, the reversing auxiliary gear 63 rotates to drive the reversing shaft 64 to rotate, the reversing shaft 64 rotates to drive the advancing gear 65 to rotate, the advancing gear 65 reversely rotates to drive the injection power gear 70 to reversely rotate, and the injection power gear 70 reversely rotates to drive the injection power shaft 71 to reversely rotate, the injection power shaft 71 rotates reversely to drive the main drive plate 76 to rotate reversely, the main drive plate 76 rotates reversely to drive the second cylindrical pin 80 to enter the wheel groove 81 in the upper grooved wheel 74 to drive the upper grooved wheel 74 to rotate one fourth reversely, the upper grooved wheel 74 drives the upper wheel shaft 75 to rotate reversely, the upper wheel shaft 75 rotates reversely to drive the upper injection gear 72 to rotate reversely, the upper injection gear 72 rotates reversely to drive the front-rear moving gear 77 to rotate reversely, the front-rear moving gear 77 rotates reversely to drive the front-rear moving shaft 78 to rotate reversely, the front-rear moving shaft 78 drives the lead screw 79 to rotate reversely, the lead screw 79 rotates reversely to drive the injection moving shell 45 to move forwards, the first cylindrical pin 80 enters the wheel groove 81 in the upper grooved wheel 74 to drive the upper grooved wheel 74 to rotate one fourth reversely, and the upper grooved wheel 74 drives the upper wheel shaft 75, the upper wheel axle 75 rotates reversely to drive the upper injection gear 72 to rotate reversely, the upper injection gear 72 rotates reversely to drive the back-and-forth moving gear 77 to rotate reversely, the back-and-forth moving gear 77 rotates reversely to drive the back-and-forth moving shaft 78 to rotate reversely, the back-and-forth moving shaft 78 drives the screw 79 to rotate reversely, the screw 79 rotates reversely to drive the injection moving shell 45 to move forward, so that the injector 40 reaches the first grid of the second row of the carrier 20, the main dial 76 rotates to drive the second cylindrical pin 80 to enter the wheel groove 81 of the lower grooved wheel 74 to drive the lower grooved wheel 74 to rotate for a quarter of a circle, the lower grooved wheel 74 drives the lower wheel axle 75 to rotate reversely, the lower wheel axle 75 drives the lower injection gear 72 to rotate for a half of a circle, the lower injection gear 72 drives the injection auxiliary gear 30 to rotate for a half of a circle, and, the outer shaft 12 rotates reversely to drive the spline shaft 13 to rotate reversely, the spline shaft 13 rotates reversely to drive the cam 14 to rotate reversely, the cam 14 rotates reversely to drive the injection pressing plate 42 to move downwards, the injection pressing plate 42 drives the injector 40 to move downwards, the injection compression spring 41 is pressed, the liquid chamber 38 injects an inducing liquid into one cell of the carrier 20 through the hose 39 and the injector 40, then the first cylindrical pin 80 enters the wheel groove 81 of the lower side sheave 74 to drive the lower side sheave 74 to rotate for a quarter of a circle, the lower side sheave 74 drives the lower side wheel shaft 75 to rotate reversely, the lower side wheel shaft 75 drives the lower side injection gear 72 to rotate reversely, the lower side injection gear 72 drives the injection auxiliary gear 30 to rotate for a half of a circle, the injection auxiliary gear 30 drives the outer shaft 12 to rotate reversely, and the outer shaft 12 rotates reversely to drive the spline shaft 13 to rotate, the spline shaft 13 rotates reversely to drive the cam 14 to rotate reversely, after the farthest off-axis point of the cam 14 leaves the injection pressure plate 42, the injection compression spring 41 rebounds and reversely rotates to drive the injection pressing plate 42 to move upwards, the injection pressing plate 42 drives the injector 40 to move upwards, the injection of the inducing liquid into the carrier 20 is stopped, the time difference is provided between the first cylindrical pin 80 and the second cylindrical pin 80 driving the lower grooved wheel 74 to rotate reversely, which is enough to inject the inducing liquid into the carrier 20, the above operations are repeated three times to complete the injection of the inducing liquid into the second row of lattices of the carrier 20, the injection moving case 45 is returned to the foremost side of the inner moving chamber 69, the moving electromagnet 67 is deenergized to release the injection housing 44, the traveling compression spring 68 rebounds to move the injection housing 44 to the left to the home position, and the injection power gear 70 engages the rearward gear 62.
The cover plate electromagnet 33 releases the moving rod 32 after losing electromagnetism, the cover plate compression spring 31 rebounds to drive the disc 82 to move downwards, the disc 82 drives the spline spindle 83 of the moving rod 32 to move downwards, the disc 82 pushes the cover plate 85 to be far away from the center of the rotary table 15 to cover the carrier 20, the cover plate 85 drives the spring plate 84 to be far away from the center of the rotary table 15, the compression spring 87 is pressed, and the protection of embryonic stem cells in the carrier 20 is completed.
When centrifugation is needed, the turntable motor 23 rotates to drive the turntable shaft 24 to rotate, the turntable shaft 24 rotates to drive the turntable 15 to rotate, and the turntable 15 rotates to drive the carrier 20 to rotate, so that centrifugation is completed.
The turntable motor 23 rotates to drive the turntable shaft 24 to rotate, the turntable shaft 24 rotates to drive the turntable 15 to rotate, the turntable 15 rotates to drive the carrier 20 to rotate, the activity detector 10 performs activity detection on embryonic stem cells in the carrier 20, when death of embryonic stem cells in the carrier 20 is found, the door motor 57 rotates clockwise to drive the door motor shaft 58 to rotate clockwise, the door motor shaft 58 rotates clockwise to drive the incomplete gear 56 to rotate clockwise, the incomplete gear 56 rotates clockwise to drive the right main gear 55 to rotate clockwise, the right main gear 55 rotates clockwise to drive the right auxiliary shaft 54 to rotate clockwise, the auxiliary shaft 54 rotates clockwise to drive the right main belt wheel 53 to rotate clockwise, and the main belt wheel 53 rotates clockwise to drive the right auxiliary belt wheel 51 to rotate clockwise through the right driving belt 52, the right side the counter pulley 51 clockwise rotates and drives the right side the door shaft 49 clockwise rotates, the right side the door shaft 49 clockwise rotates and drives the right side the door gear 47 clockwise rotates, the right side the door gear 47 clockwise rotates and drives the right side the door rack 8 upwards moves, the right side the door rack 8 drives the right side the door 6 upwards moves, the right side the door 6 drives the right side the door spring block 4 upwards moves, the right side the door spring block 4 drives the right side the door compression spring 5 to be pulled, the right side the door 6 pushes the push rod 3 to be leftwards moved, the push rod 3 drives the push rod plate 36 to be leftwards moved, the push rod compression spring 37 is pulled to complete the right side door opening, the withdrawing electromagnet 91 generates electromagnetic absorption the withdrawing rod 97 to be close to the center of the turntable 15, the withdrawing rod 97 drives the vertical rod 27 and the push block 19 to be close to the center of the turntable 15, the withdrawing compression, the torsion spring shaft 89 rotates to drive the wire wheel 94 to wind the pull wire 92, the vertical rod 27 drives the L rod 26 to be close to the center of the rotary table 15, the L rod 26 drives the limit push rod 29 to be close to the center of the rotary table 15 to release the limit of downward movement of the stop block 21, the stop block compression spring 22 rebounds to drive the stop block 21 to be downward contacted with the carrier 20 to be downward limited, at this time, the reset compression spring 98 is abutted against the wall of the exit cavity 28 close to the center of the rotary table 15, then the exit electromagnet 91 does not have electromagnetic release to the exit rod 97, the exit compression spring 93 rebounds to drive the push block 19 to be away from the center of the rotary table 15, the push block 19 drives the exit rod 97 to be away from the center of the rotary table 15, the exit rod 97 drives the upper rack 95 to be away from the center of the rotary table 15, and the upper rack 95 drives the gear 18 to rotate in, the gear 18 rotates to drive the lower rack 25 to approach the center of the rotary table 15, the lower rack 25 drives the limiting push rod 29 to approach the center of the rotary table 15, the limiting push rod 29 drives the L-rod 26 to approach the center of the rotary table 15, the return compression spring 98 is compressed until the upper rack 95 leaves the gear 18, the carrier 20 is pushed out of the channel 9, the return compression spring 98 rebounds to drive the L-rod 26 to leave the center of the rotary table 15, the L-rod 26 drives the limiting push rod 29 to move away from the center of the rotary table 15 to the original position, the push block 19 pulls the disc 82 away from the center of the rotary table 15, the disc 82 drives the reel 94 to rotate, the torsion spring shaft 89 is twisted, and during the period, the elastic coefficient difference between the torsion spring shaft 89 and the withdrawal compression spring 93 achieves the purpose that the push block 19 slowly leaves the center of the rotary table 15, ensuring that the liquid in the carrier 20 is not spilled, the door motor 57 rotates counterclockwise to drive the door motor shaft 58 to rotate counterclockwise, the door motor shaft 58 rotates counterclockwise to drive the incomplete gear 56 to rotate counterclockwise, the incomplete gear 56 rotates counterclockwise to drive the right main gear 55 to rotate counterclockwise, the right main gear 55 rotates counterclockwise to drive the right auxiliary shaft 54 to rotate counterclockwise, the auxiliary shaft 54 rotates counterclockwise to drive the right main belt pulley 53 to rotate counterclockwise, the right main belt pulley 53 rotates counterclockwise to drive the right auxiliary belt pulley 51 to rotate counterclockwise through the right transmission belt 52, the right auxiliary belt pulley 51 rotates counterclockwise to drive the right door shaft 49 to rotate counterclockwise, the right door shaft 49 rotates counterclockwise to drive the right door gear 47 to rotate counterclockwise, the right door gear 47 rotates counterclockwise to drive the right door rack 8 to move downward, the right side door rack 8 drives the right side door 6 moves down, the right side door 6 drives the right side door spring block 4 moves down, the right side door compression spring 5 recovers no load, the right side door 6 relieves the restriction of the right movement of the push rod 3, the push rod compression spring 37 rebounds to drive the push rod plate 36 to move right, the push rod plate 36 drives the push rod 3 to move right, the right side door is closed, and the carrier 20 is withdrawn.
When all the carriers 20 need to be withdrawn, the door motor 57 rotates clockwise to drive the door motor shaft 58 to rotate clockwise, the door motor shaft 58 rotates clockwise to drive the incomplete gear 56 to rotate clockwise, the incomplete gear 56 rotates clockwise to drive the right main gear 55 to rotate clockwise, the right main gear 55 rotates clockwise to drive the right auxiliary shaft 54 to rotate clockwise, the auxiliary shaft 54 rotates clockwise to drive the right main belt pulley 53 to rotate clockwise, the right main belt pulley 53 rotates clockwise to drive the right auxiliary belt pulley 51 to rotate clockwise through the right transmission belt 52, the right auxiliary belt pulley 51 rotates clockwise to drive the right door shaft 49 to rotate clockwise, the right door shaft 49 rotates clockwise to drive the right door gear 47 to rotate clockwise, the right door gear 47 rotates clockwise to drive the right door rack 8 to move upwards, the right side door rack 8 drives the right side door 6 to move upwards, the right side door 6 drives the right side door spring block 4 to move upwards, the right side door spring block 4 drives the right side door compression spring 5 to be pulled, the right side door 6 pushes the push rod 3 to move leftwards, the push rod 3 drives the push rod plate 36 to move leftwards, the push rod compression spring 37 is pulled to open the door, the withdrawing electromagnet 91 generates electromagnetic adsorption, the withdrawing rod 97 is close to the center of the circle of the turntable 15, the withdrawing rod 97 drives the vertical rod 27 and the push block 19 to be close to the center of the circle of the turntable 15, the withdrawing compression spring 93 is pressed, the torsion spring shaft 89 rotates to drive the wire wheel 94 to wind the pull wire 92, the vertical rod 27 drives the L rod 26 to be close to the center of the circle of the turntable 15, the L rod 26 drives the limit push rod 29 to be close to the center of the circle of the turntable 15, the stopper compression spring 22 rebounds to drive the stopper 21 to move downwards to contact with the carrier 20 for limiting the downward movement, at this time, the reset compression spring 98 abuts against the wall of the exit cavity 28 close to the center of the rotary table 15, then the exit electromagnet 91 releases the exit rod 97 without electromagnetism, the exit compression spring 93 rebounds to drive the push block 19 to move away from the center of the rotary table 15, the push block 19 drives the exit rod 97 to move away from the center of the rotary table 15, the exit rod 97 drives the upper rack 95 to move away from the center of the rotary table 15, the upper rack 95 drives the gear 18 to rotate during the movement in the direction away from the center of the rotary table 15, the gear 18 rotates to drive the lower rack 25 to approach the center of the rotary table 15, the lower rack 25 drives the limit push rod 29 to approach the center of the rotary table 15, and the limit push rod 29 drives the L rod 26 to approach the center of the rotary, the return compression spring 98 is compressed until the upper rack 95 leaves the gear 18, the carrier 20 is pushed out of the channel 9, the return compression spring 98 rebounds to drive the L rod 26 to be away from the center of the rotary table 15, the L rod 26 drives the limiting push rod 29 to be away from the center of the rotary table 15 to the original position, the push block 19 is away from the center of the rotary table 15 to pull the disc 82, the disc 82 drives the reel 94 to rotate, the torsion spring shaft 89 is twisted, during the period, the elastic coefficient difference between the torsion spring shaft 89 and the withdrawing compression spring 93 achieves the purpose that the push block 19 is slowly away from the center of the rotary table 15, so that the liquid in the carrier 20 is not spilled out, the rotary table motor 23 rotates to drive the rotary table shaft 24 to rotate, the rotary table shaft 24 rotates to drive the rotary table 15 to rotate, and the rotary table 15 rotates to drive the carrier 20 to rotate, repeating the above operations until all the carriers 20 exit the machine body 1, the door motor 57 rotates counterclockwise to drive the door motor shaft 58 to rotate counterclockwise, the door motor shaft 58 rotates counterclockwise to drive the incomplete gear 56 to rotate counterclockwise, the incomplete gear 56 rotates counterclockwise to drive the right main gear 55 to rotate counterclockwise, the right main gear 55 rotates counterclockwise to drive the right auxiliary shaft 54 to rotate counterclockwise, the auxiliary shaft 54 rotates counterclockwise to drive the right main belt pulley 53 to rotate counterclockwise, the main belt pulley 53 rotates counterclockwise to drive the right auxiliary belt pulley 51 to rotate counterclockwise through the right belt 52, the right auxiliary belt pulley 51 rotates counterclockwise to drive the right door shaft 49 to rotate counterclockwise, the right door shaft 49 rotates counterclockwise to drive the right door gear 47 to rotate counterclockwise, the right door gear 47 rotates counterclockwise to drive the right door rack 8 to move downward, the right side door rack 8 drives the right side door 6 moves down, the right side door 6 drives the right side door spring block 4 moves down, the right side door compression spring 5 resumes no load, the right side door 6 relieves the restriction that push rod 3 moves to the right, push rod compression spring 37 rebounds and drives push rod plate 36 moves to the right, push rod plate 36 drives push rod 3 moves to the right, the completion right side is closed the door, apron electro-magnet 33 produces the electromagnetic absorption move the carriage release lever 32 moves up, carriage release lever 32 drives disc 82 moves up, disc 82 drives spline main shaft 83 moves up, apron compression spring 31 is pressed, compression spring 87 rebounds and drives spring plate 84 is close to the 15 centre of a circle of carousel, spring plate 84 drives apron 85 is close to the 15 centre of a circle of carousel, opens carrier 20.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (5)
1. An embryonic stem cell induced differentiation instrument, which comprises a machine body, and is characterized in that: the top wall of the machine body is provided with a liquid cavity with an upward opening, the bottom wall of the liquid cavity is communicated with a hose, a push rod cavity is arranged in the bottom wall of the liquid cavity, limiting cavities are symmetrically arranged in the left wall and the right wall of the push rod cavity, a door control device for opening and closing a door only on one side by using a push rod and an incomplete gear is arranged in the limiting cavities, an incomplete gear cavity is arranged in the rear wall of the machine body, a door motor with a front output shaft is fixedly arranged in the rear wall of the incomplete gear cavity, the tail end of the output shaft of the door motor is fixedly provided with a door motor shaft extending forwards and backwards, one end of the door motor shaft far away from the door motor extends into the incomplete gear cavity, the tail end of the door motor shaft is fixedly provided with the incomplete gear, a belt wheel cavity is arranged on the front wall of the incomplete gear cavity, auxiliary shafts, one end of the auxiliary shaft far away from the main gear extends forwards into the belt wheel cavity, the tail end of the auxiliary shaft is fixedly provided with a main belt wheel, the front wall of the belt wheel cavity is bilaterally symmetrically provided with door shafts extending forwards and backwards, one end of each door shaft extending into the belt wheel cavity is fixedly provided with an auxiliary belt wheel, the bottom wall of the limiting cavity is communicated with a door cavity penetrating through the channel, the wall of the door cavity close to the door shafts is communicated with a door gear cavity, one end of each door shaft far away from the auxiliary belt wheel extends into the door gear cavity, the tail end of each door shaft is fixedly provided with a door gear, the auxiliary belt wheel is connected with the nearest main belt wheel through a transmission belt, an outer moving cavity is arranged in the bottom wall of the push rod cavity, the bottom wall of the outer moving cavity is communicated with a culture chamber, an activity detector is fixedly arranged in the right side of the top wall of the culture chamber, the end of the turntable shaft, which is far away from the turntable motor, extends upwards into the culture chamber, the tail end of the turntable shaft is fixedly provided with a turntable, the turntable top wall is provided with a carrier cavity with an upward opening around a circle center array, a carrier is placed in the carrier cavity, the bottom wall of the carrier cavity is communicated with and provided with an exit cavity, the exit cavity is internally provided with a culture device which can limit and push out the carrier through a push rod matched with a gear rack, a spline spindle for realizing extension is arranged at the center of the turntable top wall, a disc is fixedly arranged at one end of the spline spindle, which is far away from the turntable, a moving rod which extends up and down is rotationally arranged in the disc top wall, one end of the moving rod, which is far away from the disc, extends into the culture chamber top wall, an apron electromagnet is fixedly arranged above the moving rod, an apron compression spring is fixedly arranged on, the device is characterized in that a movable electromagnet is fixedly arranged on the right wall of the outer moving cavity, a movable compression spring is fixedly arranged on the right wall of the outer moving cavity, one end, far away from the right wall of the outer moving cavity, of the movable compression spring is fixedly provided with an injection shell extending leftwards and rightwards, an inner moving cavity is arranged in the injection shell, a front-back sliding injection moving shell is arranged in the inner moving cavity, a cam cavity is arranged in the injection moving shell, an injection compression spring is fixedly arranged on the bottom wall of the cam cavity, one end, far away from the bottom wall of the cam cavity, of the injection compression spring is fixedly provided with an injection pressing plate, the bottom wall of the injection pressing plate is fixedly provided with an injector, the injector extends downwards into the culture chamber, the injector is connected with the hose, a grooved wheel cavity is arranged on the rear wall of the inner moving cavity, and a dosing device which realizes that the, a reversing cavity is arranged in the rear wall of the outer moving cavity, and a power device for providing power for the dosing device is arranged in the reversing cavity.
2. An apparatus for inducing differentiation of embryonic stem cells according to claim 1, wherein: the door intracavity is provided with the door that realizes extending, the door be close to fixedly on the wall of door gear be provided with door gear engagement's door rack, the door is kept away from the fixed door spring piece that is provided with in top of the wall of door rack, the fixed door compression spring that is provided with of door spring piece diapire, door compression spring keeps away from the one end of door spring piece is fixed on the restriction chamber diapire, the push rod that extends about being provided with of push rod intracavity, stretch into both sides respectively about the push rod limit chamber and butt the door roof, the fixed push rod board that is provided with of push rod roof, the fixed push rod compression spring that is provided with of push rod board left side wall, push rod compression spring keeps away from the one end of push rod board is fixed on the push rod chamber left side wall.
3. An apparatus for inducing differentiation of embryonic stem cells according to claim 1, wherein: a stop block compression spring is fixedly arranged on the bottom wall of the withdrawing cavity far away from the center of the rotary table, a stop block is fixedly arranged at one end of the stop block compression spring far away from the bottom wall of the withdrawing cavity, a limit push rod which horizontally extends towards the center of the rotary table and is abutted against the stop block is arranged on the bottom wall of the withdrawing cavity, a lower rack is fixedly arranged on the top wall of the limit push rod, an L rod is fixedly arranged at one end of the limit push rod close to the center of the rotary table, a reset compression spring which horizontally extends is fixedly arranged at one end of the L rod close to the center of the rotary table, a gear shaft which vertically extends in the direction of the limit push rod is arranged above the lower rack, a gear which is meshed with the lower rack is fixedly arranged on the outer surface of the gear shaft, an upper rack which vertically extends in the direction of the gear shaft is meshed, the end of the withdrawing rod far away from the center of the rotary table is fixedly provided with a push block which is abutted against the carrier, the wall of the push block close to the center of the rotary table is fixedly provided with a withdrawing compression spring, the end of the withdrawing compression spring far away from the push block is fixed on the wall of the withdrawing cavity close to the center of the rotary table, the bottom wall of the end of the withdrawing rod far away from the push block is fixedly provided with an abutting joint on the wall of the L rod far away from the center of the rotary table, the wall of the withdrawing cavity close to the center of the rotary table is fixedly provided with a withdrawing electromagnet, the wall of the withdrawing cavity close to the center of the rotary table is internally provided with a speed reducing cavity, a torsion spring shaft which extends in parallel to the direction of the withdrawing rod is arranged in the speed reducing cavity, a wire wheel is fixedly arranged on the outer surface of the torsion spring shaft, the outer, the wall that the slow speed chamber is close to the carousel centre of a circle is provided with the spring chamber in, the spring chamber is kept away from fixedly on the wall in carousel centre of a circle is provided with compression spring, compression spring is close to the fixed spring plate that is provided with of one end in carousel centre of a circle, the fixed apron that is provided with of spring plate roof.
4. An apparatus for inducing differentiation of embryonic stem cells according to claim 1, wherein: the injection compression spring is characterized in that wheel shafts extending forwards and backwards are symmetrically arranged on the upper portion and the lower portion of the groove wheel cavity in an up-down mode, injection gears are fixedly arranged on the outer surface of the portion, close to the rear wall of the groove wheel cavity, of the wheel shaft, groove wheels are fixedly arranged on the outer surface of the portion, close to the front wall of the groove wheel cavity, of the wheel shaft, four groups of groove wheels are uniformly arranged on the circumferential wall of each groove wheel in a ninety degree mode, an injection power shaft extending forwards and backwards is arranged on the rear wall of the groove wheel cavity, a main driving plate is fixedly arranged at one end, extending into the groove wheel cavity, of the main driving plate, two groups of cylindrical pins are fixedly arranged on the wall of each groove wheel in a ninety degree mode, the cylindrical pins enter the groove wheels along with the rotation of the main driving plate to drive the groove wheels to rotate, a front-and-back extending outer shaft is arranged on the lower side of, the montant is kept away from the fixed one end of integral key shaft be provided with the downside the injection pinion that pours into gear engagement, the antetheca upside of groove wheel chamber is provided with the fore-and-aft movement axle that extends from beginning to end, the fore-and-aft movement axle stretches into the one end in groove wheel chamber is fixed be provided with the upside the back-and-forth movement gear that pours into gear engagement, the fore-and-aft movement axle is kept away from the one end of back-and-forth movement gear is fixed to be provided with the fore-and-aft extension and runs through remove the chamber with the lead screw of injecting into the removal shell, the lead screw with it removes shell threaded connection to inject into, the power axle is kept away.
5. An apparatus for inducing differentiation of embryonic stem cells according to claim 1, wherein: the reversing mechanism is characterized in that an injection motor with an output shaft arranged forwards is fixedly arranged in the reversing cavity rear wall, an injection motor shaft extending forwards and backwards is fixedly arranged at the tail end of an output shaft of the injection motor, the injection motor shaft is close to a reversing main gear fixedly arranged on the outer surface of the reversing cavity rear wall, a backward gear meshed with the injection power gear is fixedly arranged on the outer surface of the reversing cavity front wall, a reversing shaft extending forwards and backwards is arranged on the right side of the reversing cavity, a reversing pinion meshed with the reversing main gear is fixedly arranged on the outer surface of the reversing cavity rear wall, and a forward gear is fixedly arranged on the outer surface of the reversing cavity front wall.
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| CN202011115221.5A CN112210496A (en) | 2020-10-19 | 2020-10-19 | Embryonic stem cell induced differentiation instrument |
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| CN202011115221.5A CN112210496A (en) | 2020-10-19 | 2020-10-19 | Embryonic stem cell induced differentiation instrument |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260745A1 (en) * | 2004-05-19 | 2005-11-24 | Massachusetts Institute Of Technology | Perfused three-dimensional cell/tissue disease models |
| CN101029303A (en) * | 2006-02-28 | 2007-09-05 | 上海国睿生命科技有限公司 | Substance and method for splitting induced dry-cell to cartilage |
| CN107937340A (en) * | 2017-11-21 | 2018-04-20 | 大连医科大学附属第医院 | Catalpol is promoting application of the neural stem cell into oligodendrocyte differentiation |
| US20180282682A1 (en) * | 2017-04-04 | 2018-10-04 | Centre For Eye Research Australia | Automated system for maintenance and differentiation of pluripotent stem cells |
| CN109089424A (en) * | 2016-03-11 | 2018-12-25 | 斯特昂株式会社 | Cell rearranges range device |
| CN110684661A (en) * | 2019-11-28 | 2020-01-14 | 宁波锐风医疗器械有限公司 | But constant temperature automatic material conveying's embryo culture device |
-
2020
- 2020-10-19 CN CN202011115221.5A patent/CN112210496A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260745A1 (en) * | 2004-05-19 | 2005-11-24 | Massachusetts Institute Of Technology | Perfused three-dimensional cell/tissue disease models |
| CN101029303A (en) * | 2006-02-28 | 2007-09-05 | 上海国睿生命科技有限公司 | Substance and method for splitting induced dry-cell to cartilage |
| CN109089424A (en) * | 2016-03-11 | 2018-12-25 | 斯特昂株式会社 | Cell rearranges range device |
| US20180282682A1 (en) * | 2017-04-04 | 2018-10-04 | Centre For Eye Research Australia | Automated system for maintenance and differentiation of pluripotent stem cells |
| CN107937340A (en) * | 2017-11-21 | 2018-04-20 | 大连医科大学附属第医院 | Catalpol is promoting application of the neural stem cell into oligodendrocyte differentiation |
| CN110684661A (en) * | 2019-11-28 | 2020-01-14 | 宁波锐风医疗器械有限公司 | But constant temperature automatic material conveying's embryo culture device |
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