CN112205622A - 一种抗氧化抗衰老组合物、制备方法和应用 - Google Patents
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Abstract
本发明公开了一种抗氧化抗衰老组合物及其制备方法，抗氧化抗衰老组合物包括以下成份：玉米花粉、蜂王胎冻干粉、地龙活性蛋白、余甘果提取液、马蜂橙提取液、菌类提取液、纯水。上述抗氧化抗衰老组合物的制备方法：取余甘果提取液、马蜂橙提取液、菌类提取液混合，边搅拌边加热至90‑95℃，维持20min灭菌，然后冷却至40‑50℃，加入玉米花粉搅拌溶解，加入已灭菌的地龙蛋白提取物、蜂王胎冻干粉，补足纯水的量，搅拌混合均匀，得到抗氧化抗衰老组合物。本发明还提供了上述抗氧化抗衰老组合物在化妆品和食品中的应用。
Description
技术领域
本发明属于食品、保健食品、化妆品领域，具体涉及一种抗氧化抗衰老组合物、制备方法和应用。
背景技术
科学研究表明，癌症、衰老或其它疾病大都与过量自由基的产生有关联。衰老是一个持续的变化过程，国际主流认为自由基损伤人体细胞，从而引起众多疾病，加速人体衰老，想要延缓衰老一个重要手段就是抗氧化。抗氧化是指抗氧化自由基的简称。人体因为与外界的持续接触，包括呼吸(氧化反应)、外界污染、放射线照射等因素不断地在体内产生自由基。研究抗氧化可以有效克服其所带来的危害，所以抗氧化被食品、化妆品企业列为主要的研发方向之一，也是市场最重要的功能性诉求之一。为了满足市场需求，本方案深入挖掘、组合具有抗氧化功效的、可食用的天然原料，安全性高，可使用范围广，不仅能够应用于食品、保健食品，还能应用于化妆品等领域。本方案组合物含蜂王胎、玉米花粉、地龙蛋白等多种生物活性成份，内服可以补充或平衡人体营养，清除人体多余的自由基，修复氧化损伤，延缓人体衰老，由内而外改善皮肤状态。
发明内容
本发明的目的是提供一种抗氧化抗衰老组合物，适用于食品、保健食品、化妆品的添加使用，内外兼用，含多种活性蛋白，能够为人体补充营养，同时抗氧化、抗衰老效果显著。
进一步地，有必要提供上述抗氧化抗衰老组合物的制备方法。
一种抗氧化抗衰老组合物，包括以下成份：
玉米花粉、蜂王胎冻干粉、地龙活性蛋白、余甘果提取液、马蜂橙提取液、菌类提取液、纯水。
优选地，所述抗氧化抗衰老组合物按其重量百分比由以下成份组成：
优选地，所述抗氧化抗衰老组合物按其重量百分比由以下成份组成：
其中，所述地龙活性蛋白由以下方法制得：
取鲜地龙，洗净后，放入盛有淡盐水的玻璃盘中吐泥、排粪24小时，用剪刀剖开地龙的身体，洗去内脏与泥土，再用去离子水清洗干净，称重，按质量体积比1:3加入0.01mol/LpH7.2的Tris-Hcl缓冲溶液，4℃匀浆，4℃抽提24h，然后6000r/min离心20min，收集离心上清液进行反复冻融三次，取上清液依次用1.2μ、0.8μ、0.45μ、0.22μ微孔滤膜滤器过滤除菌，而后用中空纤维超滤柱，截留分子量为5000-10000的部分，保存于-20℃的冷柜中，使用前解冻、用0.22μ的无菌微孔滤膜器过滤除菌。
其中，所述菌类提取液由以下方法制得：
将1:1:1的香杏丽蘑、蜜蘑、大红菌粉碎混合均匀；加入30倍质量的蒸馏水，50℃、240W超声波浸提30min，然后减压过滤得到滤液，滤液加热蒸发浓缩，得到浓度100mg/ml，pH为6-7的菌类提取液，4-8℃冷藏保存。
香杏丽蘑：香杏丽蘑又称虎皮香杏、黄皮口蘑、香杏丽蘑。子实体一般中等大。菌盖半球形至平展，光滑，不粘，带白色或淡土黄色至淡土红色，边缘内卷。菌肉白色，肥厚。菌褶白色，或稍带黄色，稠密，窄，弯生，不等长。菌柄白色，或稍带黄色，具条纹，内实。夏秋季在草原上群生、丛生或形成蘑菇圈。
蜜蘑：是指榛蘑，是伞菌目白蘑科真菌，为伞菌目蜜环菌属蜜环菌的子实体，分布于中国东三省、内蒙、河北、山西、甘肃、青海、四川、浙江、云南、广西等地。蜜蘑呈伞形，淡土黄色，老后棕褐色。蜜蘑7-8月生长在针阔叶树的干基部、代根、倒木及埋在土中的枝条上。
大红菌指红菇，红菇是一类大型菌根真菌,属担子菌亚门、层菌纲、伞菌目、红菇科、红菇属。是一种名贵的野生食(药)用菌。在世界范围内分布广泛，我国主要分布于福建、云南、江西、辽宁、河南、四川、广西等省区，海拔为300——1000m山林地带，植被和土壤垂直分布明显，坡度10°-45°的缓坡地至斜坡地。
上述抗氧化抗衰老组合物的制备方法：
取余甘果提取液、马蜂橙提取液、菌类提取液混合，边搅拌边加热至90-95℃，维持20min灭菌，然后冷却至40-50℃，加入玉米花粉搅拌溶解，加入已灭菌的地龙蛋白提取物、蜂王胎冻干粉，补足纯水的量，搅拌混合均匀，得到抗氧化抗衰老组合物。
上述抗氧化抗衰老组合物在食品、保健食品和化妆品中的应用。
上述抗氧化抗衰老组合物的护肤应用方法：
1)备料：
上述抗氧化抗衰老组合物(实施例3)3-8％，马鲁拉油2-7％，玫瑰果油1-5％，橄榄油乳化蜡2-8％，超细珍珠粉2-5％，纳米二氧化钛1-3％，云母粉0.5-2％，透明质酸钠0.2-0.5％，防腐剂0.1-0.3％，纯净水61.2-88.2％；
2)制备方法
先取本方案组合物，加入透明质酸钠，混合溶解，加热至40℃得到组分A备用；称取超细珍珠粉、纳米二氧化钛、云母粉，加入马鲁拉油、玫瑰果油，浸泡研匀，加入橄榄油乳化蜡，加热混合灭菌至80℃，得到组分B；取80℃的纯净水，将组分B加入纯净水中，均质乳化3min，搅拌降温至45℃，加入组分A、防腐剂，继续搅拌混合降温至36℃即可。
上述抗氧化抗衰老组合物的食品应用方法：
1)备料：上述抗氧化抗衰老组合物3-8％，水解胶原蛋白粉5-10％，蓝莓果粉3-7％，水蜜桃果粉4-8％，赤藓糖醇1-3％，低聚果糖1-3％，魔芋胶0.1-0.3％，卡拉胶0.1-0.3％，氯化钾0.075-0.15％，乳酸钙0.05-0.10％，柠檬酸0.1-0.3％，乳酸链球菌素0.01-0.02％，食用香精0.1-0.5％，余量为纯净水。
2)制备方法：
取适量纯净水溶解水解胶原蛋白粉、蓝莓果粉、水蜜桃果粉，加入本方案组合物混合均匀得到组分A；取适量纯净水溶解柠檬酸；取适量90℃纯净水溶解乳酸钙。把赤藓糖醇、低聚果糖、魔芋胶、卡拉胶、乳酸链球菌素、氯化钾干粉混合得到糖粉，撒入搅拌的水中，搅拌溶解，加热煮沸，保温10分钟，100目筛网过滤，降温至60-70℃，加入乳酸钙溶液、柠檬酸溶液，搅拌均匀，加入组分A和食用香精，搅拌均匀，100目筛网过滤，趁热灌装封口，85℃保温15分钟进行巴氏杀菌，然后20℃水中冷却至40℃左右，用50℃热风烘干外包装，检验。
相对现有技术，本发明的制备抗氧化抗衰老组合物营养丰富、抗氧化效果显著，适用于食品、保健食品、化妆品的添加使用，内外兼用，能够为人体补充营养，抗氧化、抗衰老，保持健康美丽。
附图说明
图1为实施例、对比例ABTS+自由基清除率结果。
具体实施方式
为了使本技术领域的人员更好地理解本发明方案，下面结合具体实施方式对本发明作进一步的详细说明。
原料说明
地龙：选用广地龙，产自广东，选用长度约15-20厘米，直径约1厘米的地龙。本方案提取的地龙蛋白分子量在5000-10000，属于短链小分子物质，能顺畅的进入血管，改善微循环，保持血液健康状态，同时促进其他营养物的吸收。
玉米花粉：西安全奥生物科技有限公司，由禾本科植物玉蜀黍的雄花穗提取而得，为棕黄色粉末，80-100目，浓度10:1。玉米花粉中含有大量抗氧化物质，如：维生素、胡萝卜素及微量元素硒，可以阻止体内脂质和蛋白质过氧化反应，具有延缓衰老的作用；能使SOD的活性增强。
蜂王胎冻干粉：陕西富尔邦生物科技有限公司，由蜂王胎(蜂王幼虫)提取而得，淡黄色粉末，80目，蛋白含量30％。蜂王胎是营养成分齐全的高级天然产品，含丰富的营养物质及活性酶，可全面调节人体营养平衡。其中含有多糖、维生素A、维生素C、维生素E、超氧化物歧化酶(SOD)，硒等抗氧化成分，可以清除人体自由基；含有极其丰富的蜕皮激素和生物活性因子，有助于消除黄褐斑、雀斑等皮肤斑，可使皮肤细腻，增强皮肤表面张力，减缓、消除表皮组织松弛而形成的皱纹，美容养颜、延缓衰老。
余甘果提取液：正禾(广州)生物科技有限公司，澄清透明棕色液体，有余甘果特征气味，pH4.0-7.0，溶于水，密度0.950-1.150。余甘果提取液富含维生素C、维生素B和维生素P,还含有蛋白质、脂肪、果酸、单宁、钙、磷、钾和17种氨基酸等，为组合物中天然抗氧化成分。
马蜂橙提取液：正禾(广州)生物科技有限公司，澄清至略微浑浊液体，棕色，有马蜂橙特征气味，pH4.0-7.0，溶于水，密度0.950-1.150。马蜂橙提取液富含抗氧化成分维生素C，为组合物中天然抗氧化成分。
香杏丽蘑：市售干货，富含微量元素硒，食用后可以增强血液中谷胱甘肽过氧化酶的活性，防止过氧化物损害人体。
蜜蘑：市售干货，是传统中医药材，具有驱风活络、强筋壮骨的功能。蜜蘑富含油脂，使其含有的脂溶性维生素更易被人体吸收，对体弱、病后虚羸、易饥饿的人都有很好的补养作用。蜜蘑的维生素E含量高达36％，能有效地延缓衰老，润泽肌肤，防治血管硬化等。
大红菌：市售干货，含有人体必需的多种氨基酸，含有多糖类抗癌物质，有滋阴、补肾、润肺、活血、健脑、养颜等功效。
纯水：蒸馏水。
实施例1-3和对比例1-6
按表1所示原料配比备料，然后按以下方法制备样品：
取余甘果提取液、马蜂橙提取液、菌类提取液混合，边搅拌边加热至90-95℃，维持20min灭菌，然后冷却至40-50℃，加入玉米花粉搅拌溶解，加入已灭菌的地龙蛋白提取物、蜂王胎冻干粉，补足纯水的量，搅拌混合均匀，得到抗氧化抗衰老组合物。
表1产品配方表
1、体外总抗氧化能力测试
测试材料：总抗氧化能力检测试剂盒(ABTS法)(包含ABTS溶液、氧化剂溶液、Trolox溶液)，80％乙醇溶液，不同浓度的样品溶液，不同浓度的抗坏血酸溶液。
配制工作液:根据待测样品数量(含标准曲线)配制适量的ABTS工作液(ABTS溶液和氧化剂溶液1:1混合得到ABTS工作母液，室温避光存放12-16小时后，使用前把母液用80％乙醇溶液稀释成ABTS工作液，要求ABTS工作液的吸光度减去相应80％乙醇空白对照后，A734为0.7±0.02)
测试方法：在试管中加入3.9mlABTS工作液，分别加入0.1ml各样品溶液充分混匀，于23℃避光反应6min，然后测定混合溶液在734nm处的吸光度。以样品溶剂无水乙醇作空白对照，然后按以下公式计算ABTS+自由基清除率，将结果进行统计如图1所示。
公式中：Ac是3.9ml工作液加0.1ml样品溶液避光反应6min,734nm处的吸光度，M是3.9ml工作液加0.1ml无水乙醇避光反应6min，734nm处的吸光度。
由图1可以看出：
(1)在体外抗氧化试验中，ABTS+自由基清除率随样品浓度增大而增大，表明样品浓度越大，抗氧化能力越强；
(2)各样品中，抗坏血酸的ABTS+自由基清除效果最好，浓度0.25mg/ml可达到95％以上的清除率。
(3)本方案抗氧化抗衰老组合物实施例2(高浓度组)和实施例3(中浓度组)的ABTS+自由基清除效果较好，IC50约0.75mg/ml；高浓度组和中浓度组的效果差异不大，结合成本因素，采用中浓度较为合适。
(4)各对比例的自由基清除率有所下降，表明缺失某种成分，抗氧化效果会受影响，其中对比例6(无余甘果提取液)的清除率下降最明显，一方面是余甘果提取液的比例相对大一些，缺失该成分对整个组合的影响较大，另一方面是余甘果提取液的维生素C含量丰富，添加该提取物时抗氧化效果更强。
2、体内抗衰老能力测试
测试材料
18-25g的健康雄性小鼠55只，由江苏振林生物科技有限公司提供；D-半乳糖，常州市海拓实验室仪器有限公司提供；GSH-PX测试盒、SOD测试盒、MDA测试盒，南京全隆生物技术有限公司提供。
动物分组及给药
为了减少污染，把小鼠饲养在干净的笼子里，环境温度为20-23℃，相对湿度为45％～65％。适应环境3天后，小鼠随机分11组，每组5只，正常对照组小鼠每天在皮下注射0.9％生理盐水0.2ml，剩余各鼠每天在皮下注射0.2mlD-半乳糖[800mg/(kg·d)]，常规喂养20天，建立D-半乳糖衰老模型组。第21天开始，每日灌喂相应样品，饲养20天，样品喂养情况如表2所示。
表2
| 分组 | 给药样品 | 样品用量 |
| 正常对照组 | 0.9％生理盐水 | 0.2ml |
| 衰老模型组 | 0.9％生理盐水 | 0.2ml |
| 低浓度 | 实施例1 | 0.2ml |
| 高浓度 | 实施例2 | 0.2ml |
| 中浓度 | 实施例3 | 0.2ml |
| 无地龙蛋白组 | 对比例1 | 0.2ml |
| 无蜂王胎组 | 对比例2 | 0.2ml |
| 无玉米花粉组 | 对比例3 | 0.2ml |
| 无菌类提取液组 | 对比例4 | 0.2ml |
| 无马蜂橙组 | 对比例5 | 0.2ml |
| 无余甘果组 | 对比例6 | 0.2ml |
小鼠血清及组织样品制备与测试
完成样品喂养后，小鼠空腹处理12小时，摘除小鼠的眼球，并取得眼眶血，添加适量抗凝剂，置于离心管内3000r/min离心15min，取血清部分，4℃贮存备用。
处死小鼠，迅速取出肝脏，用冷的生理盐水洗去残留血迹，滤纸拭干水分，称重，按质量体积比(1g组织：9ml生理盐水)制成10％组织匀浆液，4℃贮存备用。
按照测试盒说明书所示测定血清SOD活性、肝脏GSH-PX活性及肝脏MDA含量。数据采用SPSS17.0软件完成统计分析，组间均值比较采用t检验，将结果列于表3中。
表3体内抗衰老测试结果
| 血清SOD活性U/ml | GSH-PX活性U/ml | MDA含量nmol/ml | |
| 正常对照组 | 118.18±0.64f | 384.38±2.64a | 2.50±0.26a |
| 衰老模型组 | 99.12±1.03h | 246.14±1.92i | 6.72±0.24g |
| 低浓度 | 113.66±1.80g | 280.48±2.29h | 5.20±0.20f |
| 中浓度 | 137.82±1.76b | 326.34±2.69c | 3.24±0.15b |
| 高浓度 | 141.26±1.07a | 366.56±5.26b | 3.04±0.17b |
| 无地龙蛋白组 | 130.22±1.12c | 288.62±1.82g | 4.20±0.10d |
| 无蜂王胎组 | 111.56±0.95g | 297.74±2.72f | 4.66±0.21e |
| 无玉米花粉组 | 125.26±1.13d | 296.10±1.59f | 3.82±0.13c |
| 无菌类提取液组 | 122.72±1.33ef | 286.68±1.81g | 4.64±0.11e |
| 无马蜂橙组 | 123.1±0.84e | 307.72±2.66d | 3.70±0.16c |
| 无余甘果组 | 121.62±0.91f | 302.94±1.47e | 4.22±0.19d |
备注：表中的数值含义为“平均值±标准差”，各组的平均值用t检验作两两比较，字母相同表示无显著差异，字母不同表示有显著性差异。
体内抗衰老结果分析
衰老组的小鼠MDA含量比正常组小鼠高，血清SOD活性和肝脏GSH-PX活性比正常组低，表明衰老模型建模成功。
本方案组合物各实施例数据与正常组、衰老组相比，均有显著差异(p<0.01)，各实施例的小鼠SOD活性、GSH-PX活性对比衰老组显著提高，各实施例的小鼠MDA值对比衰老组显著降低，表明组合物提高了小鼠的抗氧化酶活力，同时一定程度上修复小鼠衰老组织的过氧化损伤。
无蜂王胎组的小鼠SOD活性对比中浓度组相差较大，甚至与低浓度组差异不显著，无蜂王胎组的MDA值对比中浓度组相差较大，说明缺少蜂王胎的组合物的抗氧化效果大打折扣。
无菌类提取液、无马蜂橙提取液、无余甘果提取液三个实验组的SOD活性差异不显著，可能是这三种成分的添加比例接近，同时对SOD活性的作用效果相似。
无菌类提取液实验组的小鼠GSH-PX活性相对低，可能是本方案的菌类提取液硒元素较丰富，而硒元素是GSH-PX的辅助因子，因此，菌类提取液的缺失在GSH-PX方面对组合物的影响较大。无玉米花粉组和无蜂王胎组的小鼠GSH-PX活性差异不显著，因为蜂王胎含SOD酶，会增强体内抗氧化能力，玉米花粉是富硒成分，会增强GSH-PX的活性，这两种成分的缺失均会降低组合物(对比中浓度组)的抗氧化效果。这两种成分的比例相近，所以对于组合物的影响相近。
中浓度和高浓度组的MDA值差异不显著，表明两组实施例的修复效果相当，在应用中采用中浓度组即可以达到较好的效果。
实施例4：组合物在护肤品中的应用
一种抗衰老护肤膏，配料如下：
本方案组合物(实施例3)8％，马鲁拉油4％，玫瑰果油3％，橄榄油乳化蜡5％，超细珍珠粉3％，纳米二氧化钛2.5％，云母粉0.5％，透明质酸钠0.3％，防腐剂0.2％，纯净水73.7％。
制备方法
先取本方案组合物，加入透明质酸钠，混合溶解，加热至40℃得到组分A备用；称取超细珍珠粉、纳米二氧化钛、云母粉，加入马鲁拉油、玫瑰果油，浸泡研匀，加入橄榄油乳化蜡，加热混合灭菌至80℃，得到组分B；取80℃的纯净水，将组分B加入纯净水中，均质乳化3min，搅拌降温至45℃，加入组分A、防腐剂，继续搅拌混合降温至36℃即可得到护肤膏。
护肤膏的安全性测试
采用开放型人体皮肤斑贴试验.
(1)按《化妆品卫生规范》的受试者入选标准选择志愿参加试验的人员，共50人，年龄35-50岁，皮肤健康无损，无皮肤病过敏史。
(2)方法：以前臂屈侧作为受试部位，面积3×3cm2，受试部位保持干燥，
避免接触其他外用制剂，取样品0.2g每天2次均匀涂抹于受试部位，连续使用7天，同时观察皮肤反应，此过程中，若出现皮肤反应，视情况而定是否继续试验。皮肤反应按以下开放型斑贴试验皮肤反应评判标准记录结果，记录在表4中。
表4
试验结果：受试者连续使用7天样品，0例出现皮肤不良反应，说明本方案护肤膏使用安全，不会造成皮肤刺激反应。
实施例5：组合物在食品中的应用
一种抗氧化蛋白饮，配料如下：
本方案组合物(实施例3)：5％，水解胶原蛋白粉8％，蓝莓果粉4％，水蜜桃果粉6％，赤藓糖醇2.5％，低聚果糖1.5％，魔芋胶0.2％，卡拉胶0.2％，氯化钾0.1％，乳酸钙0.1％，柠檬酸0.15％，乳酸链球菌素0.02％，纯净水72.03％，食用香精0.2％。
2.制备方法：
取适量纯净水溶解水解胶原蛋白粉、蓝莓果粉、水蜜桃果粉，加入本方案组合物混合均匀得到组分A；取适量纯净水溶解柠檬酸；取适量90℃纯净水溶解乳酸钙。把赤藓糖醇、低聚果糖、魔芋胶、卡拉胶、乳酸链球菌素、氯化钾干粉混合得到糖粉，撒入搅拌的水中，搅拌溶解，加热煮沸，保温10分钟，100目筛网过滤，降温至60-70℃，加入乳酸钙溶液、柠檬酸溶液，搅拌均匀，加入组分A和食用香精，搅拌均匀，100目筛网过滤，趁热灌装封口，85℃保温15分钟进行巴氏杀菌，然后20℃水中冷却至40℃左右，用50℃热风烘干外包装，检验，得到食品组合物。
食品组合物应用的效果测试
采取人体试用样品，采用皮肤测试仪测量相应参数，从而对组合物应用的效果作出评估。
(1)受试人群：按《化妆品卫生规范》的受试者入选标准选择志愿参加试验的人员，选取女性共60人，年龄35-50岁(平均年龄40岁)，皮肤健康无损，无皮肤病过敏史，有色斑、皱纹等皮肤问题。
(2)方法：把60名志愿者分为6组，每组10人，分别为空白对照组、护肤膏组、蛋白饮组、双应用组、竞品组1、竞品组2。护肤膏组每天洁面后使用实施例4护肤膏早晚各1次；蛋白饮组每天晚上睡前1小时食用50ml实施例5制得的食品组合物(蛋白饮)；双应用组是既涂抹实施例4护肤膏又食用实施例5蛋白饮；竞品组1是每天使用某品牌贵妇膏早晚各1次；竞品组2是每天食用50ml某品牌胶原蛋白饮，空白对照组用相同量的纯净水代替涂抹的样品、饮用相同量的纯净水代替食用的样品，各组试验期间不使用其他护肤产品。采用皮肤测试仪，测试各组志愿者在第30天、第60天的角质层含水量、皱纹量、色斑量，得到平均值并对比第0天的变化结果如表5、表6和表7中。
表5角质层含水量变化(％)
| 第0天 | 第30天 | 第60天 | |
| 护肤膏组 | 0 | +15.21 | +23.62 |
| 蛋白饮组 | 0 | +17.03 | +28.54 |
| 双应用组 | 0 | +18.65 | +34.09 |
| 空白对照组 | 0 | +0.03 | +0.05 |
| 竞品组1 | 0 | +7.55 | +18.41 |
| 竞品组2 | 0 | +9.38 | +20.56 |
表6皱纹量变化(％)
表7色斑量变化
| 第0天 | 第30天 | 第60天 | |
| 护肤膏组 | 0 | -9.05 | -17.93 |
| 蛋白饮组 | 0 | -10.80 | -21.44 |
| 双应用组 | 0 | -14.89 | -30.32 |
| 空白对照组 | 0 | +0.11 | +0.19 |
| 竞品组1 | 0 | -4.60 | -8.75 |
| 竞品组2 | 0 | -7.76 | -14.06 |
对比空白组，使用产品的志愿者皮肤角质层含水量、皱纹量、色斑量有显著性变化：护肤膏组、蛋白饮组、双应用组相对竞品组1、2的护肤效果更好，双应用组的效果最好，表明护肤膏和蛋白饮一起使用效果更好；双应用组的效果仅30天就达到到竞品组60天的水平，见效更快更好，表明本方案组合物内外兼用效果好。
同时使用实施例4护肤膏和实施例5蛋白饮减少色斑效果好，通过仪器测量的色斑量显著减少，整个肤色得到均匀和提亮，组合物的抗氧化成分能够对抗人体多余的自由基，减少皮肤暗沉、色斑等老化现象，同时护肤膏中添加了二氧化钛，有防晒、防蓝光效果，能减少皮肤光老化。
对所公开的实施例的上述说明，使本领域专业技术人员能够实现或使用本发明。对这些实施例的多种修改对本领域的专业技术人员来说将是显而易见的，本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此，本发明将不会被限制于本文所示的这些实施例，而是要符合与本文所公开的原理和新颖特点相一致的最宽的范围。
Claims (7)
1.一种抗氧化抗衰老组合物，其特征在于包括以下成份：
玉米花粉、蜂王胎冻干粉、地龙活性蛋白、余甘果提取液、马蜂橙提取液、菌类提取液、纯水。
2.如权利要求1所述的抗氧化抗衰老组合物，其特征在于按其重量百分比由以下成份组成：
3.如权利要求1所述的抗氧化抗衰老组合物，其特征在于按其重量百分比由以下成份组成：
4.如权利要求1所述的抗氧化抗衰老组合物，其特征在于所述地龙活性蛋白由以下方法制得：
取鲜地龙，洗净后，放入盛有淡盐水的玻璃盘中吐泥、排粪24小时，用剪刀剖开地龙身体，洗去内脏与泥土，再用去离子水清洗干净，称重，按质量体积比1:3加入0.01mol/LpH7.2的Tris-Hcl缓冲溶液，4℃匀浆，4℃抽提24h，然后6000r/min离心20min，收集离心上清液进行反复冻融三次，取上清液依次用1.2μ、0.8μ、0.45μ、0.22μ微孔滤膜滤器过滤除菌，而后用中空纤维超滤柱，截留分子量为5000-10000的部分，保存于冷柜中(-20℃条件下)，使用前解冻、用0.22μ的无菌过滤器过滤除菌。
5.如权利要求1所述的抗氧化抗衰老组合物，其特征在于所述菌类提取液由以下方法制得：
将1:1:1香杏丽蘑、蜜蘑、大红菌粉碎混合均匀；加入30倍质量的蒸馏水，50℃、240W超声波浸提30min，然后减压过滤得到滤液，滤液加热蒸发浓缩，得到浓度100mg/ml，pH为6-7的菌类提取液，4-8℃冷藏保存。
6.一种权利要求1-5任一项所述抗氧化抗衰老组合物的制备方法：
取余甘果提取液、马蜂橙提取液、菌类提取液混合，边搅拌边加热至90-95℃，维持20min灭菌，然后冷却至40-50℃，加入玉米花粉搅拌溶解，加入已灭菌的地龙蛋白提取物、蜂王胎冻干粉，补足纯水的量，搅拌混合均匀，得到抗氧化抗衰老组合物。
7.权利要求1-5任一项所述抗氧化抗衰老组合物在食品、保健食品和化妆品中的应用。
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