CN112198323A - A detecting system for acute kidney injury - Google Patents
A detecting system for acute kidney injury Download PDFInfo
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- CN112198323A CN112198323A CN202011084544.2A CN202011084544A CN112198323A CN 112198323 A CN112198323 A CN 112198323A CN 202011084544 A CN202011084544 A CN 202011084544A CN 112198323 A CN112198323 A CN 112198323A
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- 208000009304 Acute Kidney Injury Diseases 0.000 title claims abstract description 26
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- 201000011040 acute kidney failure Diseases 0.000 title claims abstract description 26
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- 101710099550 Dickkopf-related protein 3 Proteins 0.000 claims abstract description 32
- 229940109239 creatinine Drugs 0.000 claims abstract description 17
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
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- G—PHYSICS
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
- G01N35/1083—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with one horizontal degree of freedom
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- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
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- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/34—Genitourinary disorders
- G01N2800/347—Renal failures; Glomerular diseases; Tubulointerstitial diseases, e.g. nephritic syndrome, glomerulonephritis; Renovascular diseases, e.g. renal artery occlusion, nephropathy
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Abstract
The invention discloses an inspection system for acute kidney injury, which comprises a full-automatic biochemical detector, a DKK-3 protein detector and a background system; the full-automatic biochemical detector sends detected creatinine content data to a background system, the DKK-3 protein detector provides the data to the background system, and the background system calculates and displays ratio data in a display screen.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to an inspection system for acute kidney injury.
Background
Cardiac surgery is associated with post-operative Acute Kidney Injury (AKI) and subsequent loss of renal function. Researchers examined urinary Dickkopf-3(DKK-3), a marker of tubular stress response, for the predictive role of preoperative AKI and risk of renal function loss. The RenalRIP study enrolled patients who underwent cardiac surgery and evaluated the relationship of pre-operative urinary DKK-3 concentration to creatinine ratio (DKK 3/creatinine) to post-operative AKI. In the development cohort containing 733 patients, DKK 3/creatinine concentrations in urine above 471 pg/mg were significantly correlated with increased risk of AKI (OR =1.65), a result independent of baseline renal function.
DKK-3 protein and creatinine are used as key factors for predicting acute kidney injury, in the prior art, creatinine is mainly detected by a full-automatic biochemical analyzer, and DKK-3 protein is mainly detected by a kit, so that DKK-3 protein in the prior art is relatively troublesome to detect.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides an examination system for acute kidney injury, which is mainly applied to predicting the possibility of postoperative acute kidney injury before cardiac surgery.
In order to achieve the above object, the technical solution adopted by the present invention is as follows:
an inspection system for acute kidney injury comprises a full-automatic biochemical detector, a DKK-3 protein detector and a background system; the full-automatic biochemical detector sends detected creatinine content data to a background system, the DKK-3 protein detector provides the data to the background system, and the background system calculates and displays ratio data in a display screen.
As a preferred mode, the DKK-3 protein detector is a DKK-3 protein automatic detector, which comprises a detection box body, and a refrigerating chamber, a urine processing mechanism, an operation table, a sample adding mechanism and an enzyme-labeling instrument which are arranged in the detection box body;
the detection box body is provided with a sampling port, the sampling port is connected with the urine processing mechanism through a sampling assembly, and the sampling assembly extends out of the sampling port to obtain a sterile tube filled with urine and then is sent to the urine processing mechanism;
the urine processing mechanism comprises a centrifugal mechanism and is used for carrying out centrifugal processing on the sterile tube so as to obtain supernatant in the sterile tube;
the cold storage chamber is used for storing the enzyme-labeled coated plate and is positioned on one side of the operating platform, a transmission belt is arranged in the cold storage chamber and extends to one side of the operating platform, a placing grid is arranged in the cold storage chamber and is used for placing the enzyme-labeled coated plate, and an electric push rod is arranged in the cold storage chamber and pushes the enzyme-labeled coated plate to the transmission belt; an electric push plate is arranged on one side of the operating platform, and an enzyme-labeled coating plate which moves to the operating platform along with the conveying belt is pushed to the working position of the operating platform; the refrigerating chamber is provided with a refrigerating device;
a moving mechanism is arranged between the operating platform and the urine processing mechanism, and a liquid transfer device of the moving mechanism absorbs supernatant liquid from the processed sterile tube and transports the supernatant liquid to the upper part of an enzyme-labeled coating plate of the operating platform; the operating platform is hollow, a working groove communicated with the inside of the operating platform is formed in a working position of the operating platform, a supporting block matched with the working groove in structure is arranged in the working groove, an electric rotating structure is arranged at the bottom of the supporting block, and a fifth electric telescopic column is arranged at the bottom of the electric rotating structure; the top of the supporting block is provided with an electromagnet; a support plate in sliding connection is arranged outside the enzyme-labeled coating plate, and the support plate is made of a metal material; the operating table is provided with a heating cover, a heating device is arranged between the heating cover and the working position, and the heating cover is an electric openable heating cover which can shield the working position and can not shield the working position;
the sample adding mechanism is arranged above the operating platform; the sample adding mechanism comprises a standard liquid storage box, a washing liquid storage box, an enzyme agent storage box, a color developing agent storage box and a terminator storage box; the standard liquid storage box, the washing liquid storage box, the enzyme agent storage box, the color developing agent storage box and the terminator storage box are connected to the lifting device and the sliding device; the arrangement space and the arrangement mode among the plurality of standard solution storage boxes correspond to the reaction holes on the enzyme-labeled coating plate; the washing liquid storage box has the same arrangement mode as that of a standard liquid storage box, an enzyme agent storage box, a color developing agent storage box and a terminator storage box, the standard liquid storage box is arranged in parallel with the washing liquid storage box, the enzyme agent storage box, the color developing agent storage box and the terminator storage box, namely the sliding position of the sliding device, the standard liquid storage box, the washing liquid storage box, the enzyme agent storage box, the terminator storage box or the color developing agent storage box is controlled to correspond to the position of the enzyme-labeled coating plate, and the lower part of the storage box is connected with a liquid outlet pipe through an electromagnetic valve;
a plate moving structure is arranged between the microplate reader and the operating platform, and the plate moving structure clamps and conveys the enzyme-labeled coating plate on the working position to a placing port of the microplate reader;
all the components work under the control of a controller; and the data of the microplate reader is transmitted to a background system in a wireless or wired mode.
As a preferable mode, one side of the heating cover facing to the working position is provided with a quick-drying and water-absorbing sponge.
As a preferred mode, the sampling assembly comprises a first electric guide rail and a placing block, the placing block is arranged on a sliding block of the first electric guide rail and is of an inverted L shape, a pipe groove is formed in a transverse straight part of the inverted L-shaped placing block, the transverse straight part of the inverted L-shaped placing block exceeds the sliding block of the first electric guide rail, when the sliding block slides to one end of the first electric guide rail, the end refers to one end, located at the sampling opening, of the first electric guide rail, the transverse straight part of the inverted L-shaped placing block extends out of the sampling opening, and the pipe groove which is matched with the size of the sterile pipe and has a certain depth is formed in the sliding block of the first electric guide rail and is used for placing the sterile pipe.
As a preferred mode, the urine processing mechanism comprises a grabbing hand and a centrifugal mechanism, the centrifugal mechanism comprises a centrifugal box, a centrifugal part is arranged in the centrifugal box, and an openable electric box cover is arranged at the top of the centrifugal box; the grabbing hand is located above the centrifuge box and comprises a third electric guide rail, a first electric telescopic column and a first electric clamp holder, the third electric guide rail is arranged in the detection box, the sliding block of the electric guide rail faces downwards, the first electric telescopic column is arranged on the sliding block, and the first electric clamp holder is arranged at the bottom end of the first electric telescopic column.
As a preferable mode, the moving mechanism comprises a fourth electric guide rail, a second electric telescopic column and an electric liquid transfer device. The fourth electric guide rail is arranged in the detection box body, a sliding block of the fourth electric guide rail faces downwards, the second electric telescopic column is arranged on the sliding block of the fourth electric guide rail, and an electric pipettor is arranged at the bottom end of the second electric telescopic column; the electric pipettor is used for electrically sucking liquid and releasing liquid.
Preferably, a cleaning container is arranged between the centrifugal mechanism and the operating platform, and cleaning liquid is filled in the cleaning container.
As a preferable mode, the heating cover comprises an A vertical plate, a B vertical plate, a C vertical plate, a D vertical plate and an E transverse plate; the A vertical plate is vertical to the operating platform, the arrangement direction of the A vertical plate is the same as that of the conveying belt, the A vertical plate is positioned on one side of a working position on the operating platform and is separated from the B vertical plate, the C vertical plate, the D vertical plate and the E transverse plate; the C vertical plate and the D vertical plate are arranged oppositely, a B vertical plate is connected between the C vertical plate and the D vertical plate, namely the B vertical plate and the A vertical plate are arranged oppositely, and the E transverse plate is arranged at the tops of the B vertical plate, the C vertical plate and the D vertical plate; the bottom end of the vertical plate D is intersected with the vertical plate A, the vertical plate B and the vertical plate C to be lower; a sixth electric guide rail is embedded in the operating platform and is perpendicular to the conveying belt, and the bottom of the D vertical plate is arranged on a sliding block of the sixth electric guide rail; the arrangement directions of the vertical plate D and the vertical plate C are perpendicular to the conveying belt, and the vertical plate D is farther away from the conveying belt than the vertical plate C; the working positions of the operating platform are positioned among the vertical plate A, the vertical plate B, the vertical plate C and the vertical plate D; the bottom of the vertical plate C is provided with an intensive pulley, and a circle of sealing strip is arranged on the vertical plate A; an electric heating device groove for placing an electric heating device is arranged on the operating platform between the vertical plate A and the working position.
Drawings
FIG. 1 is a top layout view of the present invention;
FIG. 2 is a bottom view of a sample addition mechanism.
Wherein the reference numerals are as follows:
1-detection box body, 2-first electric guide rail, 3-pipe groove, 4-centrifugal box, 5-box cover, 6-second electric guide rail, 7-operation table, 8-refrigerating chamber, 9-transmission belt, 10-electric push rod, 11-enzyme-labeled coating plate, 12-sixth electric guide rail, 13-fixed cover, 14-sliding cover, 15-electromagnet, 16-electric heating device, 17-absorbent cotton, 18-enzyme-labeling instrument, 19-placing port, 20-fifth electric guide rail, 21-standard solution storage box, 22-washing solution storage box, 23-enzyme agent storage box, 24-color-developing agent storage box, 25-terminator storage box and 26-cleaning container.
Detailed Description
In order to make the technical solutions better understood by those skilled in the art, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only partial embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
It should be noted that the terms "first," "second," and the like in the description and claims of this application and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It should be understood that the data so used may be interchanged under appropriate circumstances such that embodiments of the application described herein may be used. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
In this application, the terms "upper", "lower", "left", "right", "front", "rear", "top", "bottom", "inner", "outer", "middle", "vertical", "horizontal", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings. These terms are used primarily to better describe the present application and its embodiments, and are not used to limit the indicated devices, elements or components to a particular orientation or to be constructed and operated in a particular orientation.
Moreover, some of the above terms may be used to indicate other meanings besides the orientation or positional relationship, for example, the term "on" may also be used to indicate some kind of attachment or connection relationship in some cases. The specific meaning of these terms in this application will be understood by those of ordinary skill in the art as appropriate.
Furthermore, the terms "mounted," "disposed," "provided," "connected," and "sleeved" are to be construed broadly. For example, it may be a fixed connection, a removable connection, or a unitary construction; can be a mechanical connection, or an electrical connection; may be directly connected, or indirectly connected through intervening media, or may be in internal communication between two devices, elements or components. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. The present application will be described in detail below with reference to the embodiments with reference to the attached drawings.
Example 1
As shown in FIGS. 1-2, an examination system for acute kidney injury comprises a full-automatic biochemical detector, a DKK-3 protein automatic detector and a background system.
The full-automatic biochemical detector sends detected creatinine content data to the background system, and the DKK-3 protein automatic detector sends detected DKK-3 protein content data to the background system; and the background system calculates and displays the ratio data in a display screen.
The full-automatic biochemical detector in the embodiment is the prior art, and the DKK-3 protein automatic detector is an autonomous design structure.
The DKK-3 protein automatic detector comprises a detection box body 1, a controller, a refrigerating chamber 8, a urine processing mechanism, an operation table 7 and an enzyme-labeling instrument 18.
Above-mentioned urine processing mechanism sets up in detecting box 1, and 1 one side of detection box has seted up the sample connection, is equipped with sample subassembly and urine processing mechanism in the detection box 1. Specific sampling assembly is located between thief hatch and the urine processing mechanism, and sampling assembly is used for obtaining patient's urine sample from the thief hatch, then transports the urine to urine processing mechanism.
Specifically, the sampling port is provided with an electric door, and the electric door is opened or closed under the action of the controller. It should be noted that urine is typically contained in a sterile tube.
The sampling assembly comprises a first electric guide rail 2, a placing block, wherein the placing block is arranged on a sliding block of the first electric guide rail 2, the placing block is of an inverted L shape, and a transverse straight part of the inverted L-shaped placing block is provided with a pipe groove 3. The horizontal straight part of piece is placed to the type of falling L surpasss the slider of first electronic guide rail 2 for when the slider slided the one end of first electronic guide rail 2 (this end indicates the one end that first electronic guide rail 2 is located sample connection department), the horizontal straight part of piece is placed to the type of falling L stretches out the sample connection, and the medical staff of being convenient for will be equipped with the aseptic tube of urine and place in chase 3. Be equipped with on the slider of first electronic guide rail 2 with aseptic tube size matching, have the tube seat 3 of certain degree of depth for place aseptic tube, and guarantee that aseptic tube keeps vertical basically can. The sterile tubing can be fed back out of the test chamber 1 in the same manner as it enters the test chamber 1.
The urine processing mechanism comprises a grabbing hand and a centrifugal mechanism. The centrifuge mechanism of this embodiment is substantially the same as the centrifuge of the prior art, and the only difference is the cover 5. That is, the centrifugal mechanism of the present embodiment includes the centrifugal box 4, and the centrifugal component is disposed in the centrifugal box 4, and the only difference is the box cover 5, as in the centrifugal machine of the prior art. In the prior art, the box cover 5 of the centrifuge is of a folding type, in this embodiment, the top of the centrifuge box 4 is provided with a second electric guide rail 6, the length of the second electric guide rail 6 extends out from one end of the top of the centrifuge, the box cover 5 is arranged on a sliding block of the second electric guide rail 6, and the position of the sliding block on the second electric guide rail 6 is controlled, so that whether the box cover 5 is located on the centrifuge box 4 is controlled.
The grabbing hand is positioned in the detection box body 1 and above the centrifugal box 4. The grabbing hand comprises a third electric guide rail, a first electric telescopic column and a first electric clamper. The detection box body 1 is internally provided with a third electric guide rail, a sliding block of the electric guide rail faces downwards, a first electric telescopic column is arranged on the sliding block, and a first electric clamp holder is arranged at the bottom end of the first electric telescopic column. In this embodiment, the electric guide rail, the first electric telescopic column and the first electric clamp are also conventional structures, and will not be described in detail.
When the sterile tube is mounted to follow the slide on the first motor-driven rail 2 to the other end of the first motor-driven rail 2 (this end is next to the centrifuge housing 4). Snatch the electronic guide rail top shoe motion of third of hand, drive first electronic holder and move to aseptic tube top, then first electronic flexible post starts, control first electronic holder decline and grasp aseptic tube after, first electronic flexible post rises, under the effect of the electronic guide rail of third, control first electronic holder and be located 4 tops of centrifuge box, first electronic flexible post descends once more, first electronic holder is with aseptic tube place in centrifuge box 4 test tube groove 3, place the electronic guide rail 6 control case lid 5 of back of finishing and cover. Then the controller controls the centrifuge to work for 20min, then the second electric guide rail 6 controls the box cover 5 to be opened again, the urine is processed, and the urine is layered through centrifugation.
Move and get the mechanism and be located between centrifugal mechanism and operation panel 7, move and get the mechanism and include fourth electric guide rail, the electronic flexible post of second, electronic pipettor. A fourth electric guide rail is arranged in the detection box body 1, a sliding block of the fourth electric guide rail faces downwards, a second electric telescopic column is arranged on the sliding block of the fourth electric guide rail, and an electric pipettor is arranged at the bottom end of the second electric telescopic column; the electric liquid transfer device is used for electrically sucking and releasing liquid, and the electric liquid transfer device is of an existing structure and is not described in detail. The electric pipettor works under the control of the controller.
The refrigerating compartment 8 is provided on one side of the console 7, the side of the refrigerating compartment 8 being perpendicular to the fourth motorized guide rail. And the refrigerating chamber 8 is used for storing a DKK-3 protein enzyme labeling coating plate 11. The refrigerating compartment 8 is provided at a middle portion thereof with a conveyor belt 9, and the conveyor belt 9 extends from an outlet at a lower portion of the refrigerating compartment 8 to one side of the operating table 7 through. Two rows of placing grids are arranged in the refrigerating chamber 8 and on two sides of the conveying belt 9, and an enzyme-labeled coating plate 11 is placed in each row of placing grids. The placing grids are internally provided with electric push rods 10 which are used for pushing the enzyme-labeled coating plate 11 onto the conveying belt 9 and then are conveyed to one side of the operating platform 7. The bottom of the bottom plate of the specific placing grid is provided with a heat exchange structure to ensure that the bottom plate is in a low-temperature state, so as to ensure that the enzyme-labeled coating plate 11 is stored in a low-temperature environment.
Further, in this embodiment, an electric push plate is further disposed on one side of the conveyor belt 9 at the operation table 7, and is used for pushing the conveyor belt 9 to the operation table 7 with the elisa coating plate 11 moved to the operation table 7. The electric push plate comprises a third telescopic column which is transversely arranged, and an electric push rod 10 is arranged at the tail end of the telescopic column. That is, in a normal case, the third telescopic column is contracted so that the electric push rod 10 is not located on the moving path of the conveyor belt 9. When the conveying belt 9 takes the enzyme-labeled coated plate 11 to move to the operating platform 7, the third telescopic column extends, so that the electric push rod 10 is positioned on the moving path of the conveying belt 9 and positioned behind the enzyme-labeled coated plate 11, the electric push rod 10 is started at the moment, the enzyme-labeled coated plate 11 is pushed to the operating platform 7, and then the electric push rod 10 and the third telescopic column shrink and return.
A sample adding mechanism is arranged on the side opposite to the refrigerating chamber 8 above the operation table 7. The sample adding mechanism comprises a fifth electric guide rail 20, a fourth telescopic column, a standard liquid storage box 21, a washing liquid storage box 22, an enzyme agent storage box 23, a color developing agent storage box 24, a terminator storage box 25 and a liquid outlet pipe. The fifth electric guide rail 20 is fixed in the detection box body 1, and the slide block is arranged downwards. A fourth telescopic column is installed on a sliding block of the fifth electric guide rail 20, and a plurality of standard liquid storage tanks 21, a washing liquid storage tank 22, an enzyme agent storage tank 23, a color developing agent storage tank 24 and a terminator storage tank 25 are installed at the bottom end of the fourth telescopic column through an installation plate; the standard solution storage tanks 21 store standard solutions with different concentrations or different components, and the arrangement distance and the arrangement mode among the plurality of standard solution storage tanks 21 correspond to the reaction holes on the enzyme-labeled coating plate 11. The arrangement mode of the washing liquid storage box 22 is completely the same as that of the standard liquid storage box 21, the enzyme agent storage box 23, the color developing agent storage box 24 and the terminator storage box 25, and the standard liquid storage box 21 is arranged in parallel with the arrangement mode of the washing liquid storage box 22, the enzyme agent storage box 23, the color developing agent storage box 24 and the terminator storage box 25, namely the standard liquid storage box 21, the washing liquid storage box 22, the enzyme agent storage box 23, the terminator storage box 25 or the color developing agent storage box 24 are controlled to correspond to the position of the enzyme-labeled coated plate 11 through the sliding position of the fifth electric guide rail 20. The lower part of each storage box is connected with a liquid outlet pipe through an electromagnetic valve to control the liquid outlet amount.
In this embodiment, the fourth electric guide rail is to dodge the fifth electric guide rail 20, but its pipettor must be located directly over the enzyme labeling envelope plate 11, and therefore, the pipettor should be installed in the flexible column bottom of second through the connecting plate of L type for when it moved to the end of fourth electric guide rail, the electric pipettor can exceed the position at the end of the fourth electric guide rail, and be located directly over the enzyme labeling envelope plate 11.
Further, a heating cover is arranged on the operating platform 7, and for the convenience of clear description, the structure of the heating cover is disassembled into an A vertical plate, a B vertical plate, a C vertical plate, a D vertical plate and an E transverse plate; the riser A forms a fixed cover 13 of the heating cover, and the riser B, the riser C, the riser D and the transverse plate E form a sliding cover 14 of the heating cover. Wherein, A riser perpendicular to operation panel 7, and the direction that sets up of A riser is the same with transmission band 9, and A riser lies in workstation one side on 7, its and B riser, C riser, D riser, E diaphragm separation. The C riser and the D riser set up relatively, are connected with the B riser between it, and the B riser sets up with the A riser relatively promptly, and the E diaphragm setting is at the top of B riser, C riser, D riser. Wherein the bottom end of the D riser intersects the A riser, the B riser and the C riser lower. On the operation panel 7, be perpendicular to the transmission band 9 embedded sixth electronic guide rail 12 that is equipped with, the bottom of D riser sets up on the slider of sixth electronic guide rail 12. Wherein, D riser and C riser set up the direction perpendicular to transmission band 9, and its D riser is more far away from transmission band 9 than C riser.
In this embodiment, the operating position of the operating platform 7 is located between the riser A, the riser B, the riser C and the riser D. The bottom of the C vertical plate is provided with an intensive pulley, and the A vertical plate is provided with a circle of sealing strip. The integral structure formed by the vertical plate B, the vertical plate C, the vertical plate D and the transverse plate E can move towards the vertical plate A and away from the vertical plate A under the drive of a sixth electric guide rail 12, and when the integral structure moves to the vertical plate A, the integral structure is attached to the vertical plate A to form a heating cover for covering the enzyme-labeled coated plate 11, wherein an electric heating device 16 groove for placing an electric heating device 16 is formed in an operation table 7 between the vertical plate A and a working position, the electric heating device 16 is heated when the heating cover covers the enzyme-labeled coated plate 11, the heating temperature is 37 degrees, and the time is 30 minutes; after heating, the controller controls the electric heating device 16 to stop working and controls the heating cover to open.
Further, for subsequent work, the design that this embodiment has still lies in that operation panel 7 is inside hollow, and the position department of working on operation panel 7 is equipped with the work groove, and the work groove communicates with operation panel 7 is inside, is equipped with the supporting shoe that matches with the work groove structure in the work groove, and the supporting shoe bottom is equipped with electronic revolution mechanic, and electronic revolution mechanic bottom is equipped with fifth electronic flexible post. The top of the supporting block is provided with an electromagnet 15. Under normal conditions, the fifth electric telescopic column ensures that the electromagnet 15 is flush with the top of the operation table 7. The enzyme-labeled wrapper plate 11 is externally provided with a support plate in sliding connection, the support plate is made of iron materials, and when the electromagnet 15 on the support block conducts electricity, the enzyme-labeled wrapper plate 11 can be attracted through the support plate.
Quick-dry absorbent cotton 17 with thicker thickness is arranged on one side of the A vertical plate, the B vertical plate, the C vertical plate, the D vertical plate and the E transverse plate facing to the working position. In this embodiment, the cleaning mode of enzyme-labeled coated plate 11 is that, after heating cover covered enzyme-labeled coated plate 11, the fifth electric telescopic column rises by one section, then the electric rotating structure starts, drives the supporting shoe to rotate, thereby enzyme-labeled coated plate 11 follows the fast rotation, throws out liquid therein, and is caught and absorbed by the rapid-curing cutback absorbent cotton 17. It is worth emphasizing that the absorbent cotton is used for avoiding the sealing strip.
In this example, the process of detecting DKK-3 protein is roughly described, and includes the following processes:
1. the sample loading of each well of the enzyme-labeled coating plate 11 was 50. mu.l, and the concentrations were 24. mu.g/L, 16. mu.g/L, 8. mu.g/L, 4. mu.g/L, and 2. mu.g/L, respectively.
2. Blank holes are respectively arranged (the blank reference holes are not added with samples and enzyme labeling reagents, the rest steps are operated in the same way, sample holes to be detected are arranged, 40 mul of sample diluent is added into the sample holes to be detected on the enzyme labeling coating plate 11, and then 10 mul of samples to be detected are added.
3. The enzyme-labeled plate 11 was incubated at 37 ℃ for 30 minutes.
4. Discarding liquid of the enzyme-labeled coating plate 11, spin-drying, filling washing liquid into each hole, standing for 30 seconds, discarding, repeating the steps for 5 times, and patting dry.
5. 50 μ l of enzyme-labeled reagent was added to each well, except for blank wells.
6. The operation is the same as 3.
7. The operation is the same as 5.
8. The color developing agent A50 μ l and the color developing agent B50 μ l were added to each well, and color development was carried out at 37 ℃ for 15 minutes in the dark.
9. The reaction was stopped by adding 50. mu.l of stop solution to each well (blue color immediately turned yellow).
10. Assay, absorbance of each well was measured sequentially with a microplate reader 18450nm wavelength.
The steps are realized by writing the corresponding process program into the controller.
As described above, in this embodiment, since the microplate reader 18 is also required to perform the final measurement, a plate transfer mechanism is further provided on the other side of the operation table 7 corresponding to the transfer mechanism, and the microplate coated plate 11 is transferred. The plate moving mechanism is positioned between the microplate reader 18 and the operation table 7. The structure of moving the plate includes seventh electronic guide rail, seventh electronic guide rail sets up in detecting box 1, and its slider sets up down, slider bottom at seventh electronic guide rail is equipped with the flexible post of sixth, the electronic holder of second is connected through the L type connecting plate that is used for dodging fifth electronic guide rail 20 to the flexible post bottom of sixth, the electronic holder of second can be located enzyme labeling envelope board 11 top under the effect of L type connecting plate, and through the lift of the flexible post of sixth, realize moving the top of enzyme labeling envelope board 11.
The seventh motorized track extends above the port 19 of the microplate reader 18 to effect placement of the microplate coated plates 11 within the port 19 of the microplate reader 18.
The microplate reader 18 of the present embodiment is basically the same as the prior art, except that the microplate reader 18 placing port 19 is an electric cover, not a manual cover, and many electric covers capable of realizing the structure are conventional mechanical structures, and the present embodiment will not be described in detail (the electric cover may be the same as the cover 5 of the centrifuge case 4 in the present embodiment). Meanwhile, in the present embodiment, the data of the microplate reader 18 is transmitted to the background system by wireless or wired transmission.
Example 2
In this embodiment, in order to clean the electronic pipettor in embodiment 1, still be equipped with cleaning container 26 between centrifugal mechanism and operation panel 7, cleaning solution is equipped with in the cleaning container 26, when needing to clean the pipettor, electronic pipettor moves to cleaning container 26 department under the effect of fourth electric guide rail, and the electronic flexible post of second starts, and in the pipettor mouth stretched into the container was clean, under the control of controller, imbibition and flowing back were carried out to the cleaning of electronic pipettor was realized.
The invention is well implemented in accordance with the above-described embodiments. It should be noted that, based on the above structural design, in order to solve the same technical problems, even if some insubstantial modifications or colorings are made on the present invention, the adopted technical solution is still the same as the present invention, and therefore, the technical solution should be within the protection scope of the present invention.
Claims (8)
1. A checking system for acute kidney injury is characterized by comprising a full-automatic biochemical detector, a DKK-3 protein detector and a background system; the full-automatic biochemical detector sends detected creatinine content data to a background system, the DKK-3 protein detector provides the data to the background system, and the background system calculates and displays ratio data in a display screen.
2. The system for detecting acute kidney injury according to claim 1, wherein the DKK-3 protein detector is an automatic DKK-3 protein detector comprising a detection box and a cold storage chamber, a urine processing mechanism, an operation table, a sample adding mechanism and an enzyme labeling instrument which are arranged in the detection box;
the detection box body is provided with a sampling port, the sampling port is connected with the urine processing mechanism through a sampling assembly, and the sampling assembly extends out of the sampling port to obtain a sterile tube filled with urine and then is sent to the urine processing mechanism;
the urine processing mechanism comprises a centrifugal mechanism and is used for carrying out centrifugal processing on the sterile tube so as to obtain supernatant in the sterile tube;
the cold storage chamber is used for storing the enzyme-labeled coated plate and is positioned on one side of the operating platform, a transmission belt is arranged in the cold storage chamber and extends to one side of the operating platform, a placing grid is arranged in the cold storage chamber and is used for placing the enzyme-labeled coated plate, and an electric push rod is arranged in the cold storage chamber and pushes the enzyme-labeled coated plate to the transmission belt; an electric push plate is arranged on one side of the operating platform, and an enzyme-labeled coating plate which moves to the operating platform along with the conveying belt is pushed to the working position of the operating platform; the refrigerating chamber is provided with a refrigerating device;
a moving mechanism is arranged between the operating platform and the urine processing mechanism, and a liquid transfer device of the moving mechanism absorbs supernatant liquid from the processed sterile tube and transports the supernatant liquid to the upper part of an enzyme-labeled coating plate of the operating platform; the operating platform is hollow, a working groove communicated with the inside of the operating platform is formed in a working position of the operating platform, a supporting block matched with the working groove in structure is arranged in the working groove, an electric rotating structure is arranged at the bottom of the supporting block, and a fifth electric telescopic column is arranged at the bottom of the electric rotating structure; the top of the supporting block is provided with an electromagnet; a support plate in sliding connection is arranged outside the enzyme-labeled coating plate, and the support plate is made of a metal material; the operating table is provided with a heating cover, a heating device is arranged between the heating cover and the working position, and the heating cover is an electric openable heating cover which can shield the working position and can not shield the working position;
the sample adding mechanism is arranged above the operating platform; the sample adding mechanism comprises a standard liquid storage box, a washing liquid storage box, an enzyme agent storage box, a color developing agent storage box and a terminator storage box; the standard liquid storage box, the washing liquid storage box, the enzyme agent storage box, the color developing agent storage box and the terminator storage box are connected to the lifting device and the sliding device; the arrangement space and the arrangement mode among the plurality of standard solution storage boxes correspond to the reaction holes on the enzyme-labeled coating plate; the washing liquid storage box has the same arrangement mode as that of a standard liquid storage box, an enzyme agent storage box, a color developing agent storage box and a terminator storage box, the standard liquid storage box is arranged in parallel with the washing liquid storage box, the enzyme agent storage box, the color developing agent storage box and the terminator storage box, namely the sliding position of the sliding device, the standard liquid storage box, the washing liquid storage box, the enzyme agent storage box, the terminator storage box or the color developing agent storage box is controlled to correspond to the position of the enzyme-labeled coating plate, and the lower part of the storage box is connected with a liquid outlet pipe through an electromagnetic valve;
a plate moving structure is arranged between the microplate reader and the operating platform, and the plate moving structure clamps and conveys the enzyme-labeled coating plate on the working position to a placing port of the microplate reader;
all the components work under the control of a controller; and the data of the microplate reader is transmitted to a background system in a wireless or wired mode.
3. An examination system for acute kidney injury according to claim 2, wherein a quick-drying absorbent sponge is provided on a side of the heating cover facing the working site.
4. The system of claim 2, wherein the sampling assembly comprises a first electric rail, a placement block, the placement block is disposed on a sliding block of the first electric rail, the placement block is of an inverted L shape, a tube slot is disposed on a transverse portion of the inverted L-shaped placement block, the transverse portion of the inverted L-shaped placement block exceeds a sliding block of the first electric rail, so that when the sliding block slides to one end of the first electric rail, the end refers to one end of the first electric rail, which is located at the sampling port, the transverse portion of the inverted L-shaped placement block extends out of the sampling port, and the sliding block of the first electric rail is provided with a tube slot, which is matched with the size of the sterile tube, having a certain depth and used for placing the sterile tube.
5. The system of claim 2, wherein the urine processing mechanism comprises a grasping hand and a centrifugal mechanism, the centrifugal mechanism comprises a centrifugal box, a centrifugal part is arranged in the centrifugal box, and an openable electric box cover is arranged at the top of the centrifugal box; the grabbing hand is located above the centrifuge box and comprises a third electric guide rail, a first electric telescopic column and a first electric clamp holder, the third electric guide rail is arranged in the detection box, the sliding block of the electric guide rail faces downwards, the first electric telescopic column is arranged on the sliding block, and the first electric clamp holder is arranged at the bottom end of the first electric telescopic column.
6. An examination system for acute kidney injury according to claim 2, wherein the removing mechanism comprises a fourth electric rail, a second electric telescopic column, an electric pipette; the fourth electric guide rail is arranged in the detection box body, a sliding block of the fourth electric guide rail faces downwards, the second electric telescopic column is arranged on the sliding block of the fourth electric guide rail, and an electric pipettor is arranged at the bottom end of the second electric telescopic column; the electric pipettor is used for electrically sucking liquid and releasing liquid.
7. An examination system for acute kidney injury as claimed in claim 6, wherein a cleaning container is further provided between the centrifugal mechanism and the operation table, the cleaning container containing cleaning liquid.
8. An examination system for acute kidney injury according to claim 2, wherein the heating cover includes a-riser, B-riser, C-riser, D-riser and E-cross plate; the A vertical plate is vertical to the operating platform, the arrangement direction of the A vertical plate is the same as that of the conveying belt, the A vertical plate is positioned on one side of a working position on the operating platform and is separated from the B vertical plate, the C vertical plate, the D vertical plate and the E transverse plate; the C vertical plate and the D vertical plate are arranged oppositely, a B vertical plate is connected between the C vertical plate and the D vertical plate, namely the B vertical plate and the A vertical plate are arranged oppositely, and the E transverse plate is arranged at the tops of the B vertical plate, the C vertical plate and the D vertical plate; the bottom end of the vertical plate D is intersected with the vertical plate A, the vertical plate B and the vertical plate C to be lower; a sixth electric guide rail is embedded in the operating platform and is perpendicular to the conveying belt, and the bottom of the D vertical plate is arranged on a sliding block of the sixth electric guide rail; the arrangement directions of the vertical plate D and the vertical plate C are perpendicular to the conveying belt, and the vertical plate D is farther away from the conveying belt than the vertical plate C; the working positions of the operating platform are positioned among the vertical plate A, the vertical plate B, the vertical plate C and the vertical plate D; the bottom of the vertical plate C is provided with an intensive pulley, and a circle of sealing strip is arranged on the vertical plate A; an electric heating device groove for placing an electric heating device is arranged on the operating platform between the vertical plate A and the working position.
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| CN202011084544.2A CN112198323A (en) | 2020-10-12 | 2020-10-12 | A detecting system for acute kidney injury |
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| CN202011084544.2A CN112198323A (en) | 2020-10-12 | 2020-10-12 | A detecting system for acute kidney injury |
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