CN112176024B - Method for detecting bacterial inhibition of antibiotics by single cell counting - Google Patents
Method for detecting bacterial inhibition of antibiotics by single cell counting Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 64
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 58
- 239000003242 anti bacterial agent Substances 0.000 title claims abstract description 31
- 229940088710 antibiotic agent Drugs 0.000 title claims abstract description 30
- 230000005764 inhibitory process Effects 0.000 title claims abstract description 20
- 241000894006 Bacteria Species 0.000 claims abstract description 96
- 230000003115 biocidal effect Effects 0.000 claims abstract description 54
- 239000013641 positive control Substances 0.000 claims abstract description 33
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 24
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- 238000004364 calculation method Methods 0.000 claims description 5
- 238000003745 diagnosis Methods 0.000 claims 1
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- 239000003814 drug Substances 0.000 abstract description 93
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- 230000035945 sensitivity Effects 0.000 abstract description 68
- 230000008901 benefit Effects 0.000 abstract description 3
- 229960002518 gentamicin Drugs 0.000 description 23
- 238000012360 testing method Methods 0.000 description 23
- 229930182566 Gentamicin Natural products 0.000 description 22
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 22
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 22
- 239000004098 Tetracycline Substances 0.000 description 15
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- 230000012010 growth Effects 0.000 description 14
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- 241000588724 Escherichia coli Species 0.000 description 13
- 238000002474 experimental method Methods 0.000 description 13
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- 229960003376 levofloxacin Drugs 0.000 description 12
- GSDSWSVVBLHKDQ-JTQLQIEISA-N Levofloxacin Chemical compound C([C@@H](N1C2=C(C(C(C(O)=O)=C1)=O)C=C1F)C)OC2=C1N1CCN(C)CC1 GSDSWSVVBLHKDQ-JTQLQIEISA-N 0.000 description 11
- 238000001514 detection method Methods 0.000 description 11
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- 229940043263 traditional drug Drugs 0.000 description 5
- 241000194032 Enterococcus faecalis Species 0.000 description 4
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 4
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 4
- 238000009635 antibiotic susceptibility testing Methods 0.000 description 4
- 229940032049 enterococcus faecalis Drugs 0.000 description 4
- 241000588626 Acinetobacter baumannii Species 0.000 description 3
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- 241000589516 Pseudomonas Species 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229960000484 ceftazidime Drugs 0.000 description 2
- NMVPEQXCMGEDNH-TZVUEUGBSA-N ceftazidime pentahydrate Chemical compound O.O.O.O.O.S([C@@H]1[C@@H](C(N1C=1C([O-])=O)=O)NC(=O)\C(=N/OC(C)(C)C(O)=O)C=2N=C(N)SC=2)CC=1C[N+]1=CC=CC=C1 NMVPEQXCMGEDNH-TZVUEUGBSA-N 0.000 description 2
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/14—Streptococcus; Staphylococcus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
Description
Strain name | Antibiotics |
Pseudomonas aeruginosa (ATOC 27853) | Ceftazidime |
Staphylococcus aureus (ATOC 29213) | Erythromycin |
Enterococcus faecalis (ATOC 29212) | Penicillin |
Klebsiella pneumoniae | Gentamicin |
Acinetobacter baumannii | Meropenem |
Cultivation time (min) | Negative control cell count | Positive |
0 | 1636 | 1711 |
6 | 1733 | 1952 |
12 | 1814 | 2164 |
18 | 1756 | 2437 |
24 | 1737 | 2580 |
30 | 1825 | 2982 |
36 | 1970 | 3590 |
42 | 1868 | 4186 |
48 | 1733 | 4207 |
54 | 1665 | 4993 |
60 | 1871 | 5074 |
AVERAGE | 1782.545455 | |
SD | 97.99935065 | |
CV | 5.5 |
Drug name | Gradient of | Count value |
Levofloxacin | 0.008 | 69404 |
Levofloxacin | 0.015 | 69064 |
Levofloxacin | 0.03 | 59692 |
Levofloxacin | 0.06 | 9529 |
Levofloxacin | 0.12 | 6680 |
Levofloxacin | 0.25 | 8352 |
Levofloxacin | 0.5 | 8023 |
|
1 | 8443 |
|
2 | 6983 |
|
4 | 4796 |
|
8 | 3988 |
Positive control | 70413 |
Drug name | Gradient of | Count value |
Ciprofloxacin | 0.004 | 70499 |
Ciprofloxacin | 0.008 | 69458 |
Ciprofloxacin | 0.015 | 65047 |
Ciprofloxacin | 0.03 | 31476 |
Ciprofloxacin | 0.06 | 6528 |
Ciprofloxacin | 0.12 | 7461 |
Ciprofloxacin | 0.25 | 8997 |
Ciprofloxacin | 0.5 | 9502 |
|
1 | 8540 |
|
2 | 6398 |
|
4 | 6008 |
Positive control | -1 | 70413 |
Drug name | Gradient of | Count value |
Gentamicin | 0.03 | 67172 |
Gentamicin | 0.06 | 67875 |
Gentamicin | 0.12 | 60382 |
Gentamicin | 0.25 | 5901 |
Gentamicin | 0.5 | 2358 |
|
1 | 1201 |
|
2 | 1010 |
|
4 | 911 |
|
8 | 907 |
|
16 | 893 |
Positive control | 70413 |
Drug name | Gradient of | Count value |
Gentamicin | 0.03 | 67172 |
Gentamicin | 0.06 | 67875 |
Gentamicin | 0.12 | 60382 |
Gentamicin | 0.25 | 5901 |
Gentamicin | 0.5 | 2358 |
|
1 | 1201 |
|
2 | 1010 |
|
4 | 911 |
|
8 | 907 |
|
16 | 893 |
Positive control | 70413 |
Enterococcus faecalis | | 24hMIC |
Tetracycline | ||
2 | 8 |
Antibiotics | Gradient of | Count value |
Tetracycline | 0.06 | 13659 |
Tetracycline | 0.12 | 13802 |
Tetracycline | 0.25 | 11742 |
Tetracycline | 0.5 | 7912 |
|
1 | 3637 |
|
2 | 2402 |
|
4 | 1838 |
|
8 | 1768 |
|
16 | 1747 |
|
32 | 2196 |
Claims (3)
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Family Cites Families (2)
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FR2831187A1 (en) * | 2001-10-22 | 2003-04-25 | Univ Aix Marseille Ii | Determining bacteriostatic and bactericidal activity of antibiotics, by measuring amounts of bacterial DNA in test and control samples, useful for intracellular and axenic species |
CN110760559B (en) * | 2018-07-27 | 2023-11-17 | 张元珍 | Quick microorganism antibiotic sensitivity detection method |
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Title |
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流式细胞仪检测氧化亚铁硫杆菌的生长量及活性;谢丽等;《实验室科学》;20170430;第20卷(第2期);第12页第1段 * |
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