CN112129608A - Water filtering and elutriating method - Google Patents
Water filtering and elutriating method Download PDFInfo
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- CN112129608A CN112129608A CN202011078130.9A CN202011078130A CN112129608A CN 112129608 A CN112129608 A CN 112129608A CN 202011078130 A CN202011078130 A CN 202011078130A CN 112129608 A CN112129608 A CN 112129608A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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Abstract
The application discloses a water filtering and elutriating method, which comprises the following steps: step 1: adjusting the bag type filtering mechanism to a vertical station; step 2: pumping a water sample from the water sample cup into the bag type filtering mechanism by using a first peristaltic pump for enrichment, and delivering the enriched water sample in the bag type filtering mechanism to a wastewater pool; step 3, pumping the elutriation liquid into the bag type filtering mechanism from the elutriation liquid cup; and 4, step 4: performing high-frequency vibration on the bag type filtering mechanism; and 5: pumping the elutriation liquid in the bag type filtering mechanism into a sample bottle by using a second peristaltic pump; step 6: pumping the elutriation liquid from the elutriation liquid cup into the bag type filtering mechanism by using a first peristaltic pump; and 7: adjusting the bag type filtering mechanism to a horizontal station; and 8: performing high-frequency vibration on the bag type filtering mechanism; and step 9: the elutriation solution in the capsule filter mechanism is pumped into the sample vial. This application can realize the enrichment and elutriation to the water sample in step, promotes the rate of recovery of experiment to and the accuracy of experimental result.
Description
Technical Field
The application belongs to the technical field of hygiene detection, and particularly relates to a water quality filtering and elutriating method.
Background
Giardia and cryptosporidium (hereinafter collectively referred to as "two" worms) are two kinds of pathogenic protozoan parasites that seriously compromise water quality safety, and diseases caused by infection of humans and animals with the two worms, mainly transmitted through water and food, are called giardiasis and cryptosporidiosis, respectively. In the sanitary Standard for Drinking Water (GB5750-2006) of China from 7/1 of 2007, the detection limit value of two insects needs to reach less than 1/10L in the microorganism index part. In the prior art, the detection of water samples of two insects is generally completed by four steps of water sample filtration, concentration, elution, centrifugal concentration, immunomagnetic separation and dyeing microscopy. Wherein, the water sample filters concentrated elution is whole two worm detects the link at first, has the important influence to the experimental result rate of recovery, and in this step, its working process of traditional two worm experimental apparatus is as follows: during enrichment work, the bag type filtering mechanism is connected with a control valve with the pressure calibrated at 0.21MPa, 2L/min of peristalsis is additionally configured for water sample suction filtration, the bag type filtering mechanism is installed on an operation line, and an inlet and an outlet of the bag type filtering mechanism are firmly fixed by using proper pliers. The volume of the sampled water volume needs to be additionally calculated after the water sample is collected, and the water sample needs to be stored in a dark place with 4 ℃ under the storage condition, which is less than 72 hours. In the elutriation process, elutriation is carried out on the bag type filtering mechanism which is enriched and added with lauryl alcohol ether 12 elutriation liquid in the amplitude frequency of 800rpm in the 9 o 'clock direction and the 12 o' clock direction respectively in two times, the elutriation time is 10 minutes each time, the elutriation liquid in the bag type filtering mechanism is poured into a liquid receiving container each time of elutriation, the liquid adding amount of the elutriation liquid is 110ml each time, and the elutriation liquid is poured into the bag type filtering mechanism in two times in the front and back directions, and the total amount is 220 ml. Therefore, the existing equipment cannot synchronously complete two steps of sample enrichment and elutriation, so that the experimental recovery rate is greatly reduced. Therefore, how to develop a novel water quality filtering and elutriating water system can synchronously complete sample enrichment and elutriation in the process of filtering, concentrating and eluting a water sample, and improve the recovery rate and accuracy of an experiment is a direction which needs to be researched by a person skilled in the art.
Content of application
The utility model provides a water quality filtering elutriation system can realize the enrichment and elutriation to the water sample in step, promotes the rate of recovery of experiment to and the accuracy of experimental result.
The technical scheme is as follows:
a water filtration elutriation system comprising: the device comprises a bag type filtering mechanism, a station adjusting mechanism and a vibration module; the input end of the bag type filtering mechanism is connected with the first peristaltic pump, and the output end of the bag type filtering mechanism is connected with the second peristaltic pump; the first peristaltic pump is respectively communicated with the elutriation cup and the water sample cup through a three-way valve; the second peristaltic pump is respectively communicated with the sample bottle and the wastewater pool through a three-way valve; the station adjusting mechanism is connected with the bag type filtering mechanism and is used for movably switching the bag type filtering mechanism between a vertical station and a horizontal station; the vibration module is connected with the bag type filtering mechanism and used for vibrating the bag type filtering mechanism.
By adopting the technical scheme: the bag-type filtering mechanisms are respectively adjusted to a horizontal station and a vertical station by the station adjusting mechanism, and the bag-type filtering mechanisms positioned at the horizontal station and the vertical station are respectively subjected to twice oscillation elutriation by the vibration module. So that the eggs of the two insects attached to the filter membrane of 1um can be more fully elutriated out, thereby improving the recovery rate of the eggs of the two insects.
Preferably, in the water filtering and elutriating system: the bag type filtering mechanism is composed of two bag type filters connected in parallel.
By adopting the technical scheme: and by adopting a double-channel design, a parallel experiment can be realized for verifying the stability in detection. Specifically, the method comprises the following steps: the two parallel bag filters can work synchronously, one bag filter elutriates the water sample to be detected, and the other bag filter elutriates the quality control standard sample. And comparing the experimental results, and using the experimental result of the quality control standard sample to assist in verifying the stability of the result of the water sample to be tested.
Preferably, in the water filtering and elutriating system: the station adjusting mechanism comprises a motor and a fixed plate, and an output shaft of the motor is connected with one surface of the fixed plate; the bag type filtering mechanism is arranged on the other surface of the fixing plate.
By adopting the technical scheme: when the motor rotates forwards, the output shaft of the motor drives the fixing plate and the bag type filtering mechanism to rotate towards one side; when the motor rotates reversely, the output shaft of the motor drives the fixing plate and the bag type filtering mechanism to rotate towards the other side for resetting, so that the bag type filtering mechanism moves and is switched between two stations.
More preferably, in the water quality filtration and elutriation system: the station adjusting mechanism further comprises a PLC control module, and the PLC control module is electrically connected with the motor.
By adopting the technical scheme: the PLC control module is used for adjusting the positive rotation and the reverse rotation of the motor, so that the switching operation and the switching time of the bag type filtering mechanism between two stations are more accurate, and the error problem caused by manual operation is reduced.
More preferably, in the water quality filtration and elutriation system: the vibration module adopts a high-frequency vibration motor, and the vibration standard of the vibration module is preferably 3000 rpm.
More preferably, in the water quality filtration and elutriation system: the PLC control module adopts Siemens S7600.
Based on the water quality filtering and elutriating system, the invention also discloses a water quality filtering and elutriating method, which adopts the following technical scheme:
a water filtering and elutriating method comprises the following steps: step 1: adjusting the bag type filtering mechanism to a vertical station; step 2: pumping a water sample from the water sample cup into the bag type filtering mechanism for enrichment by using a first peristaltic pump, and delivering the enriched water sample in the bag type filtering mechanism to a wastewater pool by using a second peristaltic pump; and step 3: pumping the elutriation liquid from the elutriation liquid cup into the bag type filtering mechanism by using a first peristaltic pump; and 4, step 4: performing high-frequency vibration on the bag type filtering mechanism at the vertical station; and 5: pumping the elutriation liquid in the bag type filtering mechanism into a sample bottle by using a second peristaltic pump; step 6: pumping the elutriation liquid from the elutriation liquid cup into the bag type filtering mechanism by using a first peristaltic pump; and 7: adjusting the bag type filtering mechanism to a horizontal station; and 8: performing high-frequency vibration on the bag type filtering mechanism at the horizontal station; and step 9: the elutriation solution in the capsule filter mechanism is pumped into the sample bottle with a second peristaltic pump.
Preferably, in the water filtration and elutriation method, the water filtration and elutriation method further comprises: the duration of the high-frequency vibration in the step 4 and the step 8 is 3 minutes respectively.
More preferably, in the water filtration and elutriation method, the water filtration and elutriation method further comprises: the elutriation liquid adopts lauryl alcohol ether.
More preferably, in the water filtration and elutriation method, the water filtration and elutriation method further comprises: and 4, the vibration frequency of the high-frequency vibration in the steps 4 and 8 is 600 HZ.
Compared with the prior art, the application can synchronously realize the enrichment and elutriation of the water sample, and improves the recovery rate of the experiment and the accuracy of the experiment result.
Drawings
The present application will now be described in further detail with reference to the following detailed description and accompanying drawings:
fig. 1 is a schematic structural diagram of embodiment 1, in which a three-way valve and a connection line between a PLC control module and a motor are omitted;
FIG. 2 is a schematic view of the elutriation process for water samples using the present invention.
The correspondence between each reference numeral and the part name is as follows:
1. a bag filter mechanism; 2. a station adjusting mechanism; 3. a vibration module; 41. a first peristaltic pump; 42. a second peristaltic pump; 5. washing the liquid cup; 6. a sample bottle; 7. a wastewater tank; 8. a water sample cup; 9. a switch; 21. a motor; 22. a fixing plate; 23. and a PLC control module.
Detailed Description
In order to more clearly illustrate the technical solutions of the present application, the following will be further described with reference to various embodiments.
As shown in fig. 1, the embodiment 1 has the following technical scheme:
a water filtration elutriation system comprising: the device comprises a bag type filtering mechanism 1, a station adjusting mechanism 2 and a vibration module 3;
the bag type filtering mechanism 1 adopts a double-channel bag type filtering mechanism. The input end of the pump is connected with a first peristaltic pump 3, and the output end of the pump is connected with a second peristaltic pump 4;
the first peristaltic pump 3 is respectively communicated with the elutriation cup 5 and the water sample cup 8 through a three-way valve; the second peristaltic pump 4 is respectively communicated with the sample bottle 6 and the wastewater pool 7 through a three-way valve;
the station adjusting mechanism 2 is connected with the bag type filtering mechanism 1 and used for moving and switching the bag type filtering mechanism 1 between a vertical station and a horizontal station. Specifically, the method comprises the following steps: the station adjusting mechanism 2 comprises a motor 21, a fixing plate 22 and a PLC control module 23, and an output shaft of the motor 21 is connected with one surface of the fixing plate 22; the bag type filtering mechanism 1 is arranged on the other surface of the fixing plate 22; the PLC control module 23 is electrically connected to the motor 21 and configured to adjust the forward rotation and the reverse rotation of the motor 21.
The vibration module 3 is connected with the bag type filtering mechanism and used for vibrating the bag type filtering mechanism 1. Specifically, the method comprises the following steps: the vibration module adopts a high-frequency vibration motor with 3000 rpm. The PLC control module adopts Siemens S7600.
As shown in fig. 2, in practice, the working process is as follows:
a water filtering and elutriating method comprises the following steps:
step 1: putting 20L of water sample to be detected into the water sample cup 6;
step 2: the motor 21 rotates forward to drive the bag type filtering mechanism 1 on the fixing plate 22 to adjust to a vertical station, so that the liquid inlet of the bag type filtering mechanism is kept at an upward position, and the liquid outlet of the bag type filtering mechanism is kept at a downward position;
and step 3: pumping a water sample into a sac-type filtering mechanism 1 from a water sample cup 8 by using a first peristaltic pump 3 for enrichment, and intercepting giardia lamblia and cryptosporidium ova with the diameter of 5-8um contained in the water sample by using a sac-type filtering container with an internal filtering membrane with the aperture of 1 um;
and 4, step 4: discharging the water sample enriched in the bag type filtering mechanism 1 to a waste water tank 7 by using a second peristaltic pump 4;
and 5: 250ml of lauryl alcohol ether as an elutriation liquid is pumped into the bag-type filtering mechanism 1 from the elutriation liquid cup 5 by a first peristaltic pump 3 at the flow rate of 4L/min;
step 6: vibrating the vertical capsule type filtering mechanism 1 by using a high-frequency vibration motor at 3000rpm, and fully vibrating a 1um pore size filter membrane and lauryl alcohol ether elutriation liquid in the vertical capsule type filtering mechanism for 3 minutes by high-speed amplitude;
and 7: the second peristaltic pump 4 is started, and the elutriation liquid in the bag-type filtering mechanism 1 is pumped into the sample bottle 6 by the second peristaltic pump 4;
and 8: 250ml of lauryl alcohol ether as an elutriation liquid was pumped from the elutriation cup 5 into the capsule filter mechanism 1 at a flow rate of 4L/min by the first peristaltic pump 3.
And step 9: the motor 21 rotates positively to drive the bag type filtering mechanism 1 on the fixing plate to adjust to a horizontal station, so that the liquid inlet is kept at a left position and the liquid outlet is kept at a right position;
step 10: using a high-frequency vibration motor to vibrate the bag type filtering mechanism 1 in a horizontal state at a frequency of 600HZ, and fully vibrating a 1um aperture filter membrane and lauryl alcohol ether elutriation liquid in the bag type filtering mechanism for 3 minutes through high-speed amplitude;
step 11: the second peristaltic pump 4 is activated and the lauryl alcohol ether elutriation solution in the capsule filter mechanism 1 that has been sufficiently bound to the eggs of the two worms is collected into the sample bottle 6.
And (4) collecting the elutriation liquid obtained in the steps 7 and 11 to finally obtain 500ml of the lauryl alcohol ether elutriation liquid containing the two worms, thereby completing the two-worm elutriation.
In the above process: the bag filter can be used for fully elutriating the eggs of the two insects attached to the filter membrane of 1um by elutriating twice in the vertical direction and the horizontal direction. Through a plurality of experiments, the recovery rate of the two worm eggs can be improved by more than 10-15% by adopting the steps compared with a single-direction elutriation method.
The above description is only for the specific embodiment of the present application, but the scope of the present application is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present application are intended to be covered by the scope of the present application. The protection scope of the present application shall be subject to the protection scope of the claims.
Claims (4)
1. A water filtering and elutriating method comprises the following steps:
step 1: adjusting the bag type filtering mechanism to a vertical station;
step 2: pumping a water sample from the water sample cup into the bag type filtering mechanism for enrichment by using a first peristaltic pump, and delivering the enriched water sample in the bag type filtering mechanism to a wastewater pool by using a second peristaltic pump;
and step 3: pumping the elutriation liquid from the elutriation liquid cup into the bag type filtering mechanism by using a first peristaltic pump;
and 4, step 4: performing high-frequency vibration on the bag type filtering mechanism at the vertical station;
and 5: pumping the elutriation liquid in the bag type filtering mechanism into a sample bottle by using a second peristaltic pump;
step 6: pumping the elutriation liquid from the elutriation liquid cup into the bag type filtering mechanism by using a first peristaltic pump;
and 7: adjusting the bag type filtering mechanism to a horizontal station;
and 8: performing high-frequency vibration on the bag type filtering mechanism at the horizontal station;
and step 9: the elutriation solution in the capsule filter mechanism is pumped into the sample bottle with a second peristaltic pump.
2. The water filtering and elutriating method as set forth in claim 1, comprising: the duration of the high-frequency vibration in the step 4 and the step 8 is 3 minutes respectively.
3. The water filtering and elutriating method as set forth in claim 1, comprising: the elutriation liquid adopts lauryl alcohol ether.
4. The water filtering and elutriating method as set forth in claim 1, comprising: and 4, the vibration frequency of the high-frequency vibration in the steps 4 and 8 is 600 HZ.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011078130.9A CN112129608A (en) | 2020-10-10 | 2020-10-10 | Water filtering and elutriating method |
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| CN202011078130.9A CN112129608A (en) | 2020-10-10 | 2020-10-10 | Water filtering and elutriating method |
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